FvH4_6g06520

rapid alkalinization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004612
Physical Location & Seq
Forward (+)
3818620 .. 3820001
1382 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06520.t1

Sequence Viewer

Length: 327 bp
ATGGCCAAGTTACTATACTCTGTAGCAATCCTCCTGATCTGGGCCACACTCTTTCTCATGGCTTCATCATCCACCATTGAAGCTGGTGGAGACCTCTCATGGATACCCACCACCGTCTCCGGCTTAGTCTCCGACGATGAGTTCGAGCTTGACTCAGAGATCAACCGGCGCATCTTAGCCACCAGCAATTACATCAGCTACGGTGCGCTGCAGAGGAACACAGTCCCCTGCTCCAGGCGCGGCGCTTCCTACTACAATTGCAAGCCTGGGGCTCAGGCCAACCCGTATAACCGCGGCTGCAGCGCCATTACAAGGTGCAGGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

109

Amino Acids

11.81

Weight (kDa)

8.42

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 47 - 107 1.5e-29 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 240, 294
AciI CCGC 3 cut(s) 240, 292, 294
AcoI YGGCCR 1 cut(s) 3
AfiI CCNNNNNNNGG 3 cut(s) 40, 234, 312
AgsI TTSAA 1 cut(s) 80
AjnI CCWGG 2 cut(s) 233, 265
AluBI AGCT 3 cut(s) 83, 148, 198
AluI AGCT 3 cut(s) 83, 148, 198
Alw26I GTCTC 3 cut(s) 84, 121, 133
AoxI GGCC 3 cut(s) 3, 42, 276
ApeKI GCWGC 3 cut(s) 208, 297, 300
ArsI GACNNNNNNTTYG 2 cut(s) 125, 157
AspLEI GCGC 5 cut(s) 171, 208, 240, 245, 305
AspS9I GGNCC 1 cut(s) 42
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 274
BbvI GCAGC 3 cut(s) 195, 284, 312
BciT130I CCWGG 2 cut(s) 235, 267
BciVI GTATCC 1 cut(s) 96
BcoDI GTCTC 3 cut(s) 84, 121, 133
BfmI CTRYAG 3 cut(s) 21, 209, 298
BfoI RGCGCY 2 cut(s) 246, 306
BfuI GTATCC 1 cut(s) 96
BisI GCNGC 5 cut(s) 209, 241, 295, 298, 301
BlsI GCNGC 5 cut(s) 210, 242, 296, 299, 302
Bme1390I CCNGG 2 cut(s) 235, 267
BmgT120I GGNCC 1 cut(s) 42
BmrFI CCNGG 2 cut(s) 235, 267
BmsI GCATC 1 cut(s) 180
BplI GAGNNNNNCTC 2 cut(s) 137, 169
BpmI CTGGAG 1 cut(s) 217
Bpu10I CCTNAGC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 266, 292
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 234, 312
Bse118I RCCGGY 1 cut(s) 165
BseBI CCWGG 2 cut(s) 235, 267
BseDI CCNNGG 2 cut(s) 266, 292
BseGI GGATG 1 cut(s) 68
BseLI CCNNNNNNNGG 3 cut(s) 40, 234, 312
BseMII CTCAG 2 cut(s) 168, 287
BseXI GCAGC 3 cut(s) 195, 284, 312
Bsh1236I CGCG 2 cut(s) 240, 294
BshFI GGCC 3 cut(s) 5, 44, 278
BsiSI CCGG 2 cut(s) 120, 166
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 3 cut(s) 40, 234, 312
BsmAI GTCTC 3 cut(s) 84, 121, 133
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 3 cut(s) 5, 44, 278
Bso31I GGTCTC 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 274
Bsp143I GATC 2 cut(s) 36, 159
BspACI CCGC 3 cut(s) 240, 292, 294
BspANI GGCC 3 cut(s) 5, 44, 278
BspCNI CTCAG 2 cut(s) 167, 286
BspFNI CGCG 2 cut(s) 240, 294
BspMAI CTGCAG 2 cut(s) 213, 302
BspTNI GGTCTC 1 cut(s) 84
BsrFI RCCGGY 1 cut(s) 165
BssAI RCCGGY 1 cut(s) 165
BssECI CCNNGG 2 cut(s) 266, 292
BssMI GATC 2 cut(s) 36, 159
Bst2UI CCWGG 2 cut(s) 235, 267
Bst4CI ACNGT 3 cut(s) 115, 203, 223
BstC8I GCNNGC 1 cut(s) 263
BstDEI CTNAG 4 cut(s) 124, 154, 175, 273
BstDSI CCRYGG 1 cut(s) 292
BstENI CCTNNNNNAGG 1 cut(s) 232
BstF5I GGATG 1 cut(s) 68
BstFNI CGCG 2 cut(s) 240, 294
BstH2I RGCGCY 2 cut(s) 246, 306
BstHHI GCGC 5 cut(s) 171, 208, 240, 245, 305
BstKTI GATC 2 cut(s) 39, 162
BstMAI GTCTC 3 cut(s) 84, 121, 133
BstMBI GATC 2 cut(s) 36, 159
BstMWI GCNNNNNNNGC 2 cut(s) 237, 300
BstNI CCWGG 2 cut(s) 235, 267
BstSCI CCNGG 2 cut(s) 233, 265
BstSFI CTRYAG 3 cut(s) 21, 209, 298
BstUI CGCG 2 cut(s) 240, 294
BstV1I GCAGC 3 cut(s) 195, 284, 312
BsuI GTATCC 1 cut(s) 96
BsuRI GGCC 3 cut(s) 5, 44, 278
BtgI CCRYGG 1 cut(s) 292
BtsCI GGATG 1 cut(s) 68
Cac8I GCNNGC 1 cut(s) 263
CfoI GCGC 5 cut(s) 171, 208, 240, 245, 305
Cfr10I RCCGGY 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 42
Cfr42I CCGCGG 1 cut(s) 295
CviAII CATG 2 cut(s) 58, 99
DdeI CTNAG 4 cut(s) 124, 154, 175, 273
DpnI GATC 2 cut(s) 38, 161
DpnII GATC 2 cut(s) 36, 159
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 1 cut(s) 274
Eco31I GGTCTC 1 cut(s) 84
EcoNI CCTNNNNNAGG 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 233, 265
EcoT38I GRGCYC 1 cut(s) 274
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 2 cut(s) 61, 102
FaiI YATR 4 cut(s) 16, 59, 100, 288
FaqI GGGAC 1 cut(s) 209
FatI CATG 2 cut(s) 57, 98
Fnu4HI GCNGC 5 cut(s) 209, 241, 295, 298, 301
FokI GGATG 1 cut(s) 55
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 5 cut(s) 209, 241, 295, 298, 301
GlaI GCGC 5 cut(s) 170, 207, 239, 244, 304
GluI GCNGC 5 cut(s) 209, 241, 295, 298, 301
GsuI CTGGAG 1 cut(s) 217
HaeII RGCGCY 2 cut(s) 246, 306
HaeIII GGCC 3 cut(s) 5, 44, 278
HapII CCGG 2 cut(s) 120, 166
HhaI GCGC 5 cut(s) 171, 208, 240, 245, 305
Hin1II CATG 2 cut(s) 61, 102
Hin6I GCGC 5 cut(s) 169, 206, 238, 243, 303
HinP1I GCGC 5 cut(s) 169, 206, 238, 243, 303
HinfI GANTC 1 cut(s) 152
HpaII CCGG 2 cut(s) 120, 166
Hpy188I TCNGA 2 cut(s) 133, 157
Hpy188III TCNNGA 1 cut(s) 34
Hpy99I CGWCG 1 cut(s) 137
HpyCH4III ACNGT 3 cut(s) 115, 203, 223
HpyCH4V TGCA 4 cut(s) 211, 261, 300, 318
HpyF10VI GCNNNNNNNGC 2 cut(s) 237, 300
HpyF3I CTNAG 4 cut(s) 124, 154, 175, 273
Hsp92II CATG 2 cut(s) 61, 102
HspAI GCGC 5 cut(s) 169, 206, 238, 243, 303
KspI CCGCGG 1 cut(s) 295
Kzo9I GATC 2 cut(s) 36, 159
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 3 cut(s) 195, 284, 312
LweI GCATC 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 9
MalI GATC 2 cut(s) 38, 161
MboI GATC 2 cut(s) 36, 159
MfeI CAATTG 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 274
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 187, 256
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 3 cut(s) 41, 104, 207
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 325
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 2 cut(s) 120, 166
MspR9I CCNGG 2 cut(s) 235, 267
MteI GCGCNGCGC 1 cut(s) 241
MunI CAATTG 1 cut(s) 256
MvaI CCWGG 2 cut(s) 235, 267
MvnI CGCG 2 cut(s) 240, 294
MwoI GCNNNNNNNGC 2 cut(s) 237, 300
NdeII GATC 2 cut(s) 36, 159
NlaIII CATG 2 cut(s) 61, 102
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PkrI GCNGC 5 cut(s) 210, 242, 296, 299, 302
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
Psp6I CCWGG 2 cut(s) 233, 265
PspGI CCWGG 2 cut(s) 233, 265
PspPI GGNCC 1 cut(s) 42
PstI CTGCAG 2 cut(s) 213, 302
SacII CCGCGG 1 cut(s) 295
SaqAI TTAA 1 cut(s) 325
SatI GCNGC 5 cut(s) 209, 241, 295, 298, 301
Sau3AI GATC 2 cut(s) 36, 159
Sau96I GGNCC 1 cut(s) 42
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 235, 267
SduI GDGCHC 1 cut(s) 274
SetI ASST 5 cut(s) 85, 96, 150, 200, 317
SfaNI GCATC 1 cut(s) 180
SfcI CTRYAG 3 cut(s) 21, 209, 298
Sfr303I CCGCGG 1 cut(s) 295
SgrBI CCGCGG 1 cut(s) 295
Sse9I AATT 2 cut(s) 187, 256
SsiI CCGC 3 cut(s) 240, 292, 294
StyD4I CCNGG 2 cut(s) 233, 265
TaaI ACNGT 3 cut(s) 115, 203, 223
TaqI TCGA 1 cut(s) 144
TasI AATT 2 cut(s) 187, 256
TauI GCSGC 2 cut(s) 243, 297
Tru1I TTAA 1 cut(s) 325
Tru9I TTAA 1 cut(s) 325
TseI GCWGC 3 cut(s) 208, 297, 300
TspDTI ATGAA 1 cut(s) 54
XagI CCTNNNNNAGG 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.