MD03G1171400.v1.1

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004612
Physical Location & Seq
Reverse (-)
23288852 .. 23289022
171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1171400.v1.1.491

Sequence Viewer

Length: 171 bp
ATGGACTCGGAGATCAGCCGTCGCCTTTTAGCCACCACCAAGTACATCAACTACGGTGCACTGCAGAGGAACACCATGCCTTGCTCCAGGTGCGGCGCCTCCTACTACAATTGCAAGCCCGGTGCTCAGGCCAACCCTTCCGTTTTTCCAAGAAAATGCTGGAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

57

Amino Acids

6.39

Weight (kDa)

9.69

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 1 - 46 4.2e-18 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 95
AciI CCGC 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 1 cut(s) 44
AjnI CCWGG 1 cut(s) 86
Alw21I GWGCWC 2 cut(s) 61, 127
Alw44I GTGCAC 1 cut(s) 57
AoxI GGCC 1 cut(s) 129
ApaLI GTGCAC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 98
AsuC2I CCSGG 1 cut(s) 120
BaeGI GKGCMC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 95
Bbv12I GWGCWC 2 cut(s) 61, 127
BceAI ACGGC 1 cut(s) 3
BciT130I CCWGG 1 cut(s) 88
BcnI CCSGG 1 cut(s) 120
BfmI CTRYAG 1 cut(s) 62
BfoI RGCGCY 1 cut(s) 99
BisI GCNGC 1 cut(s) 94
BlsI GCNGC 1 cut(s) 95
Bme1390I CCNGG 2 cut(s) 88, 120
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 2 cut(s) 88, 120
BpmI CTGGAG 1 cut(s) 70
Bpu10I CCTNAGC 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 120
BsaHI GRCGYC 1 cut(s) 96
BseBI CCWGG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 140
BseSI GKGCMC 1 cut(s) 61
BshFI GGCC 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 2 cut(s) 61, 127
BsiSI CCGG 1 cut(s) 120
BsnI GGCC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 61, 127
Bsp143I GATC 1 cut(s) 12
BspACI CCGC 1 cut(s) 93
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 97
BspMAI CTGCAG 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 95
BssMI GATC 1 cut(s) 12
BssNI GRCGYC 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 88
Bst4CI ACNGT 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 96
BstC8I GCNNGC 1 cut(s) 116
BstDEI CTNAG 1 cut(s) 126
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 1 cut(s) 98
BstKTI GATC 1 cut(s) 15
BstMBI GATC 1 cut(s) 12
BstMWI GCNNNNNNNGC 1 cut(s) 90
BstNI CCWGG 1 cut(s) 88
BstSCI CCNGG 2 cut(s) 86, 118
BstSFI CTRYAG 1 cut(s) 62
BstSLI GKGCMC 1 cut(s) 61
BsuRI GGCC 1 cut(s) 131
BtsI GCAGTG 1 cut(s) 59
BtsIMutI CAGTG 1 cut(s) 59
Cac8I GCNNGC 1 cut(s) 116
CfoI GCGC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 43
CviAII CATG 1 cut(s) 76
CviJI RGCY 4 cut(s) 18, 32, 118, 131
CviKI_1 RGCY 4 cut(s) 18, 32, 118, 131
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 1 cut(s) 126
DinI GGCGCC 1 cut(s) 97
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 86
EgeI GGCGCC 1 cut(s) 97
EheI GGCGCC 1 cut(s) 97
FaeI CATG 1 cut(s) 79
FaiI YATR 1 cut(s) 77
FatI CATG 1 cut(s) 75
Fnu4HI GCNGC 1 cut(s) 94
Fsp4HI GCNGC 1 cut(s) 94
GlaI GCGC 1 cut(s) 97
GluI GCNGC 1 cut(s) 94
GsuI CTGGAG 1 cut(s) 70
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 1 cut(s) 131
HapII CCGG 1 cut(s) 120
HhaI GCGC 1 cut(s) 98
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 1 cut(s) 79
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 120
Hpy166II GTNNAC 1 cut(s) 59
Hpy188I TCNGA 1 cut(s) 10
Hpy8I GTNNAC 1 cut(s) 59
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 1 cut(s) 147
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4V TGCA 3 cut(s) 59, 64, 114
HpyF10VI GCNNNNNNNGC 1 cut(s) 90
HpyF3I CTNAG 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 1 cut(s) 79
HspAI GCGC 1 cut(s) 96
KasI GGCGCC 1 cut(s) 95
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 89
LpnPI CCDG 5 cut(s) 73, 100, 113, 133, 145
MalI GATC 1 cut(s) 14
MboI GATC 1 cut(s) 12
MfeI CAATTG 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 61, 127
MluCI AATT 1 cut(s) 109
Mly113I GGCGCC 1 cut(s) 96
MnlI CCTC 2 cut(s) 60, 109
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 2 cut(s) 88, 120
MunI CAATTG 1 cut(s) 109
MvaI CCWGG 1 cut(s) 88
MwoI GCNNNNNNNGC 1 cut(s) 90
NarI GGCGCC 1 cut(s) 96
NciI CCSGG 1 cut(s) 120
NdeII GATC 1 cut(s) 12
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 1 cut(s) 97
PkrI GCNGC 1 cut(s) 95
PluTI GGCGCC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 86
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 97
PstI CTGCAG 1 cut(s) 66
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SatI GCNGC 1 cut(s) 94
Sau3AI GATC 1 cut(s) 12
ScrFI CCNGG 2 cut(s) 88, 120
SduI GDGCHC 2 cut(s) 61, 127
SetI ASST 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 62
SfoI GGCGCC 1 cut(s) 97
Sse9I AATT 1 cut(s) 109
SsiI CCGC 1 cut(s) 93
SspDI GGCGCC 1 cut(s) 95
StyD4I CCNGG 2 cut(s) 86, 118
TaaI ACNGT 1 cut(s) 56
TasI AATT 1 cut(s) 109
TatI WGTACW 1 cut(s) 42
TauI GCSGC 1 cut(s) 96
TscAI CASTG 1 cut(s) 66
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 1 cut(s) 66
VneI GTGCAC 1 cut(s) 57
XcmI CCANNNNNNNNNTGG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.