Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004612

Physical Location & Seq

Genomic Coordinates Reverse (-)

14066505 .. 14066888

Gene Structure 384 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1169200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 384 bp

ATGGGAAGCTCAAGCTCAAGAACTCCATCACTCTTTGGCTGCATCTTCATCTGCGCAGTCCTGGCCGCCCATTTGGTAGTGATTGCTTCAGCTTCGGCCGTTGATTTCAGCGGGGGCCACCAGCTGGGCGACCTGAGCTGGGCTTCGACGAGATCGGCGGCGGGGGGACTAATAACTGACGAGGACGAGGATGCGGAGATGCAGATGGACTCGGAGATCAACCGGCGCATCTTAGCCACGAGCAGGTACATTAGCTACGGTGCGTTGAGGAGGAACACTATCCCCTGCTCGCGACGTGGCGCGTCCTACTACAACTGCCGACCTGGGGCTCCGGCAAATCCCTACCGTCGCGGCTGTAGCTCCATTACTCGCTGCAGGCGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005911 GO:0007154 GO:0007165 GO:0007267 GO:0008150 GO:0009506 GO:0009966 GO:0009987 GO:0010469 GO:0010646 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030054 GO:0030545 GO:0035556 GO:0048018 GO:0048046 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0065009 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RALF

Protein Analysis

128

Amino Acids

13.68

Weight (kDa)

9.12

Isoelectric Point (pI)

55.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RALF	PF05498	66 - 127	1.8e-29	Rapid ALkalinization Factor (RALF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004612)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15800
fragaria_vesca	FvH4_4g18001 FvH4_6g06520
malus_domestica	MD03G1171400.v1.1 MD04G1201100.v1.1 MD12G1213600.v1.1 MD13G1168800.v1.1 MD16G1169200.v1.1
prunus_persica	Prupe.1G137000_v2.0.a1 Prupe.6G321200_v2.0.a1
pyrus_communis	pycom12g19950 pycom13g14440 pycom16g14230 pycom16g14250
rosa_chinensis	RchiOBHm_Chr3g0455671 RchiOBHm_Chr4g0422211
rosa_multiflora	Rmu_sc0002883.1_g000009 Rmu_sc0014362.1_g000002 Rmu_sc0026117.1_g000002
rosa_roxburghii	Rroxscaffold_5G00364330 Rroxscaffold_6G00423610 Rroxscaffold_6G00424110
rosa_rugosa	Rorug03G0009000 Rorug04G0178800
rosa_samantha	Rh3AG068700 Rh3BG071200 Rh3CG069500 Rh3DG071100 Rh4CG252800
rosa_wichuraiana	Rw3G005510 Rw4G020570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	55
Acc36I	ACCTGC	1 cut(s)	234
AccB7I	CCANNNNNTGG	1 cut(s)	124
AccII	CGCG	3 cut(s)	292, 302, 351
AciI	CCGC	6 cut(s)	66, 111, 158, 161, 194, 351
AcoI	YGGCCR	2 cut(s)	63, 96
AcuI	CTGAAG	1 cut(s)	72
AfaI	GTAC	1 cut(s)	248
AfiI	CCNNNNNNNGG	4 cut(s)	124, 139, 243, 325
AjiI	CACGTC	1 cut(s)	296
AjnI	CCWGG	2 cut(s)	60, 322
AluBI	AGCT	7 cut(s)	9, 15, 92, 124, 138, 255, 360
AluI	AGCT	7 cut(s)	9, 15, 92, 124, 138, 255, 360
AoxI	GGCC	3 cut(s)	63, 96, 115
ApeKI	GCWGC	2 cut(s)	39, 372
AspLEI	GCGC	4 cut(s)	56, 228, 302, 381
AspS9I	GGNCC	1 cut(s)	115
BanII	GRGCYC	1 cut(s)	331
BauI	CACGAG	1 cut(s)	238
BbvI	GCAGC	2 cut(s)	26, 359
BccI	CCATC	2 cut(s)	34, 199
BceAI	ACGGC	1 cut(s)	83
BciT130I	CCWGG	2 cut(s)	62, 324
BfmI	CTRYAG	2 cut(s)	355, 373
BfoI	RGCGCY	1 cut(s)	382
BfuAI	ACCTGC	1 cut(s)	234
BisI	GCNGC	5 cut(s)	40, 66, 159, 352, 373
BlsI	GCNGC	5 cut(s)	41, 67, 160, 353, 374
Bme1390I	CCNGG	2 cut(s)	62, 324
BmgBI	CACGTC	1 cut(s)	296
BmgT120I	GGNCC	1 cut(s)	115
BmiI	GGNNCC	2 cut(s)	116, 330
BmrFI	CCNGG	2 cut(s)	62, 324
BmsI	GCATC	4 cut(s)	51, 181, 189, 237
Bpu10I	CCTNAGC	1 cut(s)	134
BsaJI	CCNNGG	1 cut(s)	323
Bsc4I	CCNNNNNNNGG	4 cut(s)	124, 139, 243, 325
Bse118I	RCCGGY	1 cut(s)	222
BseBI	CCWGG	2 cut(s)	62, 324
BseDI	CCNNGG	1 cut(s)	323
BseGI	GGATG	1 cut(s)	196
BseLI	CCNNNNNNNGG	4 cut(s)	124, 139, 243, 325
BseMII	CTCAG	1 cut(s)	125
BseRI	GAGGAG	1 cut(s)	283
BseX3I	CGGCCG	1 cut(s)	96
BseXI	GCAGC	2 cut(s)	26, 359
BseYI	CCCAGC	2 cut(s)	124, 138
Bsh1236I	CGCG	3 cut(s)	292, 302, 351
Bsh1285I	CGRYCG	1 cut(s)	99
BshFI	GGCC	3 cut(s)	65, 98, 117
BsiEI	CGRYCG	1 cut(s)	99
BsiSI	CCGG	2 cut(s)	223, 332
BslFI	GGGAC	1 cut(s)	180
BslI	CCNNNNNNNGG	4 cut(s)	124, 139, 243, 325
BsmFI	GGGAC	1 cut(s)	180
BsnI	GGCC	3 cut(s)	65, 98, 117
Bsp1286I	GDGCHC	1 cut(s)	331
Bsp143I	GATC	2 cut(s)	152, 216
Bsp68I	TCGCGA	1 cut(s)	292
BspACI	CCGC	6 cut(s)	66, 111, 158, 161, 194, 351
BspANI	GGCC	3 cut(s)	65, 98, 117
BspCNI	CTCAG	1 cut(s)	126
BspFNI	CGCG	3 cut(s)	292, 302, 351
BspLI	GGNNCC	2 cut(s)	116, 330
BspMAI	CTGCAG	1 cut(s)	377
BspMI	ACCTGC	1 cut(s)	234
BsrFI	RCCGGY	1 cut(s)	222
BssAI	RCCGGY	1 cut(s)	222
BssECI	CCNNGG	1 cut(s)	323
BssMI	GATC	2 cut(s)	152, 216
BssSI	CACGAG	1 cut(s)	238
Bst2BI	CACGAG	1 cut(s)	238
Bst2UI	CCWGG	2 cut(s)	62, 324
Bst4CI	ACNGT	2 cut(s)	260, 347
BstC8I	GCNNGC	2 cut(s)	290, 377
BstDEI	CTNAG	2 cut(s)	134, 232
BstF5I	GGATG	1 cut(s)	196
BstFNI	CGCG	3 cut(s)	292, 302, 351
BstH2I	RGCGCY	1 cut(s)	382
BstHHI	GCGC	4 cut(s)	56, 228, 302, 381
BstKTI	GATC	2 cut(s)	155, 219
BstMBI	GATC	2 cut(s)	152, 216
BstMCI	CGRYCG	1 cut(s)	99
BstMWI	GCNNNNNNNGC	4 cut(s)	62, 135, 357, 378
BstNI	CCWGG	2 cut(s)	62, 324
BstSCI	CCNGG	2 cut(s)	60, 322
BstSFI	CTRYAG	2 cut(s)	355, 373
BstUI	CGCG	3 cut(s)	292, 302, 351
BstV1I	GCAGC	2 cut(s)	26, 359
BstZI	CGGCCG	1 cut(s)	96
BsuRI	GGCC	3 cut(s)	65, 98, 117
BtrI	CACGTC	1 cut(s)	296
BtsCI	GGATG	1 cut(s)	196
BtuMI	TCGCGA	1 cut(s)	292
BveI	ACCTGC	1 cut(s)	234
Cac8I	GCNNGC	2 cut(s)	290, 377
CfoI	GCGC	4 cut(s)	56, 228, 302, 381
Cfr10I	RCCGGY	1 cut(s)	222
Cfr13I	GGNCC	1 cut(s)	115
CseI	GACGC	1 cut(s)	291
Csp6I	GTAC	1 cut(s)	247
CviQI	GTAC	1 cut(s)	247
DdeI	CTNAG	2 cut(s)	134, 232
DpnI	GATC	2 cut(s)	154, 218
DpnII	GATC	2 cut(s)	152, 216
EaeI	YGGCCR	2 cut(s)	63, 96
EagI	CGGCCG	1 cut(s)	96
EclXI	CGGCCG	1 cut(s)	96
Eco24I	GRGCYC	1 cut(s)	331
Eco52I	CGGCCG	1 cut(s)	96
Eco57I	CTGAAG	1 cut(s)	72
EcoRII	CCWGG	2 cut(s)	60, 322
EcoT38I	GRGCYC	1 cut(s)	331
FaqI	GGGAC	1 cut(s)	180
FauI	CCCGC	2 cut(s)	104, 154
Fnu4HI	GCNGC	5 cut(s)	40, 66, 159, 352, 373
FokI	GGATG	1 cut(s)	203
FriOI	GRGCYC	1 cut(s)	331
Fsp4HI	GCNGC	5 cut(s)	40, 66, 159, 352, 373
FspI	TGCGCA	1 cut(s)	55
GlaI	GCGC	4 cut(s)	55, 227, 301, 380
GluI	GCNGC	5 cut(s)	40, 66, 159, 352, 373
GsaI	CCCAGC	2 cut(s)	128, 142
HaeII	RGCGCY	1 cut(s)	382
HaeIII	GGCC	3 cut(s)	65, 98, 117
HapII	CCGG	2 cut(s)	223, 332
HgaI	GACGC	1 cut(s)	291
HhaI	GCGC	4 cut(s)	56, 228, 302, 381
Hin6I	GCGC	4 cut(s)	54, 226, 300, 379
HinP1I	GCGC	4 cut(s)	54, 226, 300, 379
HinfI	GANTC	1 cut(s)	209
HpaII	CCGG	2 cut(s)	223, 332
Hpy188I	TCNGA	1 cut(s)	214
Hpy188III	TCNNGA	2 cut(s)	18, 291
Hpy99I	CGWCG	3 cut(s)	151, 297, 351
HpyCH4III	ACNGT	2 cut(s)	260, 347
HpyCH4IV	ACGT	1 cut(s)	295
HpyCH4V	TGCA	3 cut(s)	42, 202, 375
HpyF10VI	GCNNNNNNNGC	4 cut(s)	62, 135, 357, 378
HpyF3I	CTNAG	2 cut(s)	134, 232
HpySE526I	ACGT	1 cut(s)	295
HspAI	GCGC	4 cut(s)	54, 226, 300, 379
Kzo9I	GATC	2 cut(s)	152, 216
LmnI	GCTCC	2 cut(s)	334, 365
Lsp1109I	GCAGC	2 cut(s)	26, 359
LweI	GCATC	4 cut(s)	51, 181, 189, 237
MaeII	ACGT	1 cut(s)	295
MalI	GATC	2 cut(s)	154, 218
MboI	GATC	2 cut(s)	152, 216
MboII	GAAGA	1 cut(s)	37
MhlI	GDGCHC	1 cut(s)	331
MlyI	GAGTC	1 cut(s)	203
MnlI	CCTC	4 cut(s)	175, 181, 261, 264
MspA1I	CMGCKG	2 cut(s)	111, 124
MspI	CCGG	2 cut(s)	223, 332
MspR9I	CCNGG	2 cut(s)	62, 324
MvaI	CCWGG	2 cut(s)	62, 324
MvnI	CGCG	3 cut(s)	292, 302, 351
MwoI	GCNNNNNNNGC	4 cut(s)	62, 135, 357, 378
NdeII	GATC	2 cut(s)	152, 216
NlaIV	GGNNCC	2 cut(s)	116, 330
NruI	TCGCGA	1 cut(s)	292
NsbI	TGCGCA	1 cut(s)	55
PflMI	CCANNNNNTGG	1 cut(s)	124
PkrI	GCNGC	5 cut(s)	41, 67, 160, 353, 374
PleI	GAGTC	1 cut(s)	203
PpsI	GAGTC	1 cut(s)	203
Psp6I	CCWGG	2 cut(s)	60, 322
PspFI	CCCAGC	2 cut(s)	124, 138
PspGI	CCWGG	2 cut(s)	60, 322
PspN4I	GGNNCC	2 cut(s)	116, 330
PspPI	GGNCC	1 cut(s)	115
PstI	CTGCAG	1 cut(s)	377
PvuII	CAGCTG	1 cut(s)	124
RruI	TCGCGA	1 cut(s)	292
RsaI	GTAC	1 cut(s)	248
RsaNI	GTAC	1 cut(s)	247
SatI	GCNGC	5 cut(s)	40, 66, 159, 352, 373
Sau3AI	GATC	2 cut(s)	152, 216
Sau96I	GGNCC	1 cut(s)	115
SchI	GAGTC	1 cut(s)	203
ScrFI	CCNGG	2 cut(s)	62, 324
SduI	GDGCHC	1 cut(s)	331
SfaNI	GCATC	4 cut(s)	51, 181, 189, 237
SfcI	CTRYAG	2 cut(s)	355, 373
SmlI	CTYRAG	2 cut(s)	10, 16
SmoI	CTYRAG	2 cut(s)	10, 16
SsiI	CCGC	6 cut(s)	66, 111, 158, 161, 194, 351
StyD4I	CCNGG	2 cut(s)	60, 322
TaaI	ACNGT	2 cut(s)	260, 347
TaiI	ACGT	1 cut(s)	298
TaqI	TCGA	1 cut(s)	146
TauI	GCSGC	3 cut(s)	68, 161, 354
TseI	GCWGC	2 cut(s)	39, 372
TspDTI	ATGAA	1 cut(s)	37
Van91I	CCANNNNNTGG	1 cut(s)	124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.