Rmu_sc0026117.1_g000002

rapid alkalinization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0026117.1
OG0004612
Physical Location & Seq
Reverse (-)
7702 .. 7974
273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0026117.1_g000002.1.cds

Sequence Viewer

Length: 273 bp
atggcttcatcttccactgttgaagcaagtggagaccttccgtggatacccaccaccgccaccggcttagtctccgggaacgatgagttcgaccttgactcggagatcaaccggcgcatcttagccaccagcggctacatcagctacggtgcactgcagaggaacactgtcccttgctccaggcgcggcgcctcctactacaattgccagcctggggcccaggccaacccctatagccgcggctgcagcgccatcacaaggtgcaggagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

90

Amino Acids

9.61

Weight (kDa)

7.64

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AccII CGCG 2 cut(s) 186, 240
AciI CCGC 5 cut(s) 57, 132, 186, 238, 240
AcyI GRCGYC 1 cut(s) 189
AdeI CACNNNGTG 1 cut(s) 261
AfiI CCNNNNNNNGG 3 cut(s) 100, 214, 258
AgsI TTSAA 1 cut(s) 23
AjnI CCWGG 3 cut(s) 179, 211, 219
AluBI AGCT 1 cut(s) 144
AluI AGCT 1 cut(s) 144
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 2 cut(s) 27, 76
Alw44I GTGCAC 1 cut(s) 150
AoxI GGCC 2 cut(s) 216, 222
ApaI GGGCCC 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 243, 246
AspLEI GCGC 4 cut(s) 117, 186, 191, 251
AspS9I GGNCC 2 cut(s) 216, 217
AsuC2I CCSGG 1 cut(s) 76
BaeGI GKGCMC 2 cut(s) 154, 220
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 2 cut(s) 230, 258
BccI CCATC 1 cut(s) 260
BciT130I CCWGG 3 cut(s) 181, 213, 221
BciVI GTATCC 1 cut(s) 39
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 27, 76
BfmI CTRYAG 3 cut(s) 155, 232, 244
BfoI RGCGCY 2 cut(s) 192, 252
BfuI GTATCC 1 cut(s) 39
BisI GCNGC 6 cut(s) 133, 187, 238, 241, 244, 247
BlsI GCNGC 6 cut(s) 134, 188, 239, 242, 245, 248
Bme1390I CCNGG 4 cut(s) 76, 181, 213, 221
BmgT120I GGNCC 2 cut(s) 216, 217
BmiI GGNNCC 3 cut(s) 190, 217, 218
BmrFI CCNGG 4 cut(s) 76, 181, 213, 221
BmsI GCATC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 163
BpuMI CCSGG 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 27
BsaJI CCNNGG 4 cut(s) 41, 212, 219, 238
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 214, 258
Bse118I RCCGGY 2 cut(s) 62, 111
BseBI CCWGG 3 cut(s) 181, 213, 221
BseDI CCNNGG 4 cut(s) 41, 212, 219, 238
BseLI CCNNNNNNNGG 3 cut(s) 100, 214, 258
BseSI GKGCMC 2 cut(s) 154, 220
BseXI GCAGC 2 cut(s) 230, 258
Bsh1236I CGCG 2 cut(s) 186, 240
BshFI GGCC 2 cut(s) 218, 224
BshNI GGYRCC 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 154
BsiSI CCGG 3 cut(s) 63, 75, 112
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 3 cut(s) 100, 214, 258
BsmAI GTCTC 2 cut(s) 27, 76
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 2 cut(s) 218, 224
Bso31I GGTCTC 1 cut(s) 27
Bsp120I GGGCCC 1 cut(s) 216
Bsp1286I GDGCHC 2 cut(s) 154, 220
Bsp143I GATC 1 cut(s) 105
BspACI CCGC 5 cut(s) 57, 132, 186, 238, 240
BspANI GGCC 2 cut(s) 218, 224
BspFNI CGCG 2 cut(s) 186, 240
BspLI GGNNCC 3 cut(s) 190, 217, 218
BspMAI CTGCAG 2 cut(s) 159, 248
BspT107I GGYRCC 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 27
BsrFI RCCGGY 2 cut(s) 62, 111
BssAI RCCGGY 2 cut(s) 62, 111
BssECI CCNNGG 4 cut(s) 41, 212, 219, 238
BssMI GATC 1 cut(s) 105
BssNI GRCGYC 1 cut(s) 189
Bst2UI CCWGG 3 cut(s) 181, 213, 221
Bst4CI ACNGT 3 cut(s) 19, 149, 169
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 209
BstDEI CTNAG 2 cut(s) 67, 121
BstDSI CCRYGG 2 cut(s) 41, 238
BstFNI CGCG 2 cut(s) 186, 240
BstH2I RGCGCY 2 cut(s) 192, 252
BstHHI GCGC 4 cut(s) 117, 186, 191, 251
BstKTI GATC 1 cut(s) 108
BstMAI GTCTC 2 cut(s) 27, 76
BstMBI GATC 1 cut(s) 105
BstMWI GCNNNNNNNGC 4 cut(s) 141, 183, 243, 246
BstNI CCWGG 3 cut(s) 181, 213, 221
BstSCI CCNGG 4 cut(s) 74, 179, 211, 219
BstSFI CTRYAG 3 cut(s) 155, 232, 244
BstSLI GKGCMC 2 cut(s) 154, 220
BstUI CGCG 2 cut(s) 186, 240
BstV1I GCAGC 2 cut(s) 230, 258
BsuI GTATCC 1 cut(s) 39
BsuRI GGCC 2 cut(s) 218, 224
BtgI CCRYGG 2 cut(s) 41, 238
BtsI GCAGTG 1 cut(s) 152
BtsIMutI CAGTG 3 cut(s) 15, 152, 165
Cac8I GCNNGC 1 cut(s) 209
CfoI GCGC 4 cut(s) 117, 186, 191, 251
Cfr10I RCCGGY 2 cut(s) 62, 111
Cfr13I GGNCC 2 cut(s) 216, 217
Cfr42I CCGCGG 1 cut(s) 241
DdeI CTNAG 2 cut(s) 67, 121
DinI GGCGCC 1 cut(s) 190
DpnI GATC 1 cut(s) 107
DpnII GATC 1 cut(s) 105
DraIII CACNNNGTG 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 220
Eco31I GGTCTC 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 216
EcoRII CCWGG 3 cut(s) 179, 211, 219
EcoT38I GRGCYC 1 cut(s) 220
EgeI GGCGCC 1 cut(s) 190
EheI GGCGCC 1 cut(s) 190
FaiI YATR 1 cut(s) 234
FaqI GGGAC 1 cut(s) 155
Fnu4HI GCNGC 6 cut(s) 133, 187, 238, 241, 244, 247
FriOI GRGCYC 1 cut(s) 220
Fsp4HI GCNGC 6 cut(s) 133, 187, 238, 241, 244, 247
GlaI GCGC 4 cut(s) 116, 185, 190, 250
GluI GCNGC 6 cut(s) 133, 187, 238, 241, 244, 247
GsuI CTGGAG 1 cut(s) 163
HaeII RGCGCY 2 cut(s) 192, 252
HaeIII GGCC 2 cut(s) 218, 224
HapII CCGG 3 cut(s) 63, 75, 112
HhaI GCGC 4 cut(s) 117, 186, 191, 251
Hin1I GRCGYC 1 cut(s) 189
Hin6I GCGC 4 cut(s) 115, 184, 189, 249
HinP1I GCGC 4 cut(s) 115, 184, 189, 249
HinfI GANTC 1 cut(s) 98
HpaII CCGG 3 cut(s) 63, 75, 112
Hpy166II GTNNAC 1 cut(s) 152
Hpy188I TCNGA 1 cut(s) 103
Hpy8I GTNNAC 1 cut(s) 152
HpyAV CCTTC 1 cut(s) 47
HpyCH4III ACNGT 3 cut(s) 19, 149, 169
HpyCH4V TGCA 4 cut(s) 152, 157, 246, 264
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 183, 243, 246
HpyF3I CTNAG 2 cut(s) 67, 121
Hsp92I GRCGYC 1 cut(s) 189
HspAI GCGC 4 cut(s) 115, 184, 189, 249
KasI GGCGCC 1 cut(s) 188
KspI CCGCGG 1 cut(s) 241
Kzo9I GATC 1 cut(s) 105
LmnI GCTCC 1 cut(s) 182
Lsp1109I GCAGC 2 cut(s) 230, 258
LweI GCATC 1 cut(s) 126
MalI GATC 1 cut(s) 107
MboI GATC 1 cut(s) 105
MboII GAAGA 1 cut(s) 3
MfeI CAATTG 1 cut(s) 202
MhlI GDGCHC 2 cut(s) 154, 220
MluCI AATT 1 cut(s) 202
Mly113I GGCGCC 1 cut(s) 189
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 2 cut(s) 153, 202
MseI TTAA 1 cut(s) 271
MspA1I CMGCKG 2 cut(s) 132, 240
MspI CCGG 3 cut(s) 63, 75, 112
MspR9I CCNGG 4 cut(s) 76, 181, 213, 221
MteI GCGCNGCGC 1 cut(s) 187
MunI CAATTG 1 cut(s) 202
MvaI CCWGG 3 cut(s) 181, 213, 221
MvnI CGCG 2 cut(s) 186, 240
MwoI GCNNNNNNNGC 4 cut(s) 141, 183, 243, 246
NarI GGCGCC 1 cut(s) 189
NciI CCSGG 1 cut(s) 76
NdeII GATC 1 cut(s) 105
NlaIV GGNNCC 3 cut(s) 190, 217, 218
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfoI TCCNGGA 1 cut(s) 74
PkrI GCNGC 6 cut(s) 134, 188, 239, 242, 245, 248
PleI GAGTC 1 cut(s) 92
PluTI GGCGCC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 92
Psp6I CCWGG 3 cut(s) 179, 211, 219
PspGI CCWGG 3 cut(s) 179, 211, 219
PspN4I GGNNCC 3 cut(s) 190, 217, 218
PspOMI GGGCCC 1 cut(s) 216
PspPI GGNCC 2 cut(s) 216, 217
PstI CTGCAG 2 cut(s) 159, 248
SacII CCGCGG 1 cut(s) 241
SaqAI TTAA 1 cut(s) 271
SatI GCNGC 6 cut(s) 133, 187, 238, 241, 244, 247
Sau3AI GATC 1 cut(s) 105
Sau96I GGNCC 2 cut(s) 216, 217
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 4 cut(s) 76, 181, 213, 221
SduI GDGCHC 2 cut(s) 154, 220
SetI ASST 4 cut(s) 39, 96, 146, 263
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 3 cut(s) 155, 232, 244
SfoI GGCGCC 1 cut(s) 190
Sfr303I CCGCGG 1 cut(s) 241
SgrBI CCGCGG 1 cut(s) 241
Sse9I AATT 1 cut(s) 202
SsiI CCGC 5 cut(s) 57, 132, 186, 238, 240
SspDI GGCGCC 1 cut(s) 188
StyD4I CCNGG 4 cut(s) 74, 179, 211, 219
TaaI ACNGT 3 cut(s) 19, 149, 169
TaqI TCGA 1 cut(s) 90
TasI AATT 1 cut(s) 202
TauI GCSGC 4 cut(s) 135, 189, 240, 243
Tru1I TTAA 1 cut(s) 271
Tru9I TTAA 1 cut(s) 271
TscAI CASTG 3 cut(s) 22, 159, 172
TseI GCWGC 2 cut(s) 243, 246
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 3 cut(s) 22, 159, 172
VneI GTGCAC 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.