Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0004612

Physical Location & Seq

Genomic Coordinates Forward (+)

44600504 .. 44601466

Gene Structure 963 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G020570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 354 bp

ATGGCAAGCTCCTCCTCCTCCTCCTCACTGCTACTCTTCGGCTTCATCTTCCTCTGTTCAATCCTGGCCGCCCATTCCACTCTCGCCGCCGCTTCCACTTCCGCCGTCGATTTCAGCTGGGGCTCCGCCGGCGGAGGGCTGATAGGTGATTTGGATGAGGAGCTGGAGCTGCAGATGGACTCGGAGATCAACCGGCGCATCTTAGCCACCACCAGGTACATTAGCTACGGTGCGCTGAGGAGGAACACTGTCCCCTGCTCGCGACGCGGCGCCTCGTACTACAATTGCCGTCCCGGAGCTCAGGCCAACCCCTACTCGCGCGGCTGTAGCCGCATCACTCGCTGCAGGCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005911 GO:0007154 GO:0007165 GO:0007267 GO:0008150 GO:0009506 GO:0009966 GO:0009987 GO:0010469 GO:0010646 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030054 GO:0030545 GO:0035556 GO:0048018 GO:0048046 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0065009 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RALF

Protein Analysis

117

Amino Acids

12.62

Weight (kDa)

9.01

Isoelectric Point (pI)

54.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RALF	PF05498	56 - 116	1.7e-29	Rapid ALkalinization Factor (RALF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004612)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15800
fragaria_vesca	FvH4_4g18001 FvH4_6g06520
malus_domestica	MD03G1171400.v1.1 MD04G1201100.v1.1 MD12G1213600.v1.1 MD13G1168800.v1.1 MD16G1169200.v1.1
prunus_persica	Prupe.1G137000_v2.0.a1 Prupe.6G321200_v2.0.a1
pyrus_communis	pycom12g19950 pycom13g14440 pycom16g14230 pycom16g14250
rosa_chinensis	RchiOBHm_Chr3g0455671 RchiOBHm_Chr4g0422211
rosa_multiflora	Rmu_sc0002883.1_g000009 Rmu_sc0014362.1_g000002 Rmu_sc0026117.1_g000002
rosa_roxburghii	Rroxscaffold_5G00364330 Rroxscaffold_6G00423610 Rroxscaffold_6G00424110
rosa_rugosa	Rorug03G0009000 Rorug04G0178800
rosa_samantha	Rh3AG068700 Rh3BG071200 Rh3CG069500 Rh3DG071100 Rh4CG252800
rosa_wichuraiana	Rw3G005510 Rw4G020570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	269
AccII	CGCG	4 cut(s)	262, 267, 319, 321
AciI	CCGC	9 cut(s)	69, 87, 90, 102, 126, 132, 267, 321, 331
AcoI	YGGCCR	1 cut(s)	66
AcyI	GRCGYC	1 cut(s)	270
AfaI	GTAC	2 cut(s)	218, 278
AfiI	CCNNNNNNNGG	2 cut(s)	135, 213
AgsI	TTSAA	1 cut(s)	60
AjnI	CCWGG	2 cut(s)	63, 212
AluBI	AGCT	6 cut(s)	9, 117, 163, 169, 225, 299
AluI	AGCT	6 cut(s)	9, 117, 163, 169, 225, 299
Alw21I	GWGCWC	1 cut(s)	301
AoxI	GGCC	2 cut(s)	66, 303
ApeKI	GCWGC	2 cut(s)	169, 342
AspLEI	GCGC	4 cut(s)	198, 235, 272, 321
AsuC2I	CCSGG	1 cut(s)	294
AsuHPI	GGTGA	1 cut(s)	158
BanI	GGYRCC	1 cut(s)	269
BanII	GRGCYC	2 cut(s)	125, 301
Bbv12I	GWGCWC	1 cut(s)	301
BbvCI	CCTCAGC	1 cut(s)	236
BbvI	GCAGC	2 cut(s)	156, 329
BccI	CCATC	1 cut(s)	169
BceAI	ACGGC	2 cut(s)	89, 273
BciT130I	CCWGG	2 cut(s)	65, 214
BcnI	CCSGG	1 cut(s)	294
BfmI	CTRYAG	3 cut(s)	170, 325, 343
BfoI	RGCGCY	1 cut(s)	273
BisI	GCNGC	8 cut(s)	69, 87, 90, 170, 268, 322, 331, 343
BlsI	GCNGC	8 cut(s)	70, 88, 91, 171, 269, 323, 332, 344
Bme1390I	CCNGG	3 cut(s)	65, 214, 294
BmiI	GGNNCC	2 cut(s)	124, 271
BmrFI	CCNGG	3 cut(s)	65, 214, 294
BmsI	GCATC	2 cut(s)	207, 342
BpmI	CTGGAG	1 cut(s)	185
Bpu10I	CCTNAGC	2 cut(s)	236, 300
BpuMI	CCSGG	1 cut(s)	294
BsaHI	GRCGYC	1 cut(s)	270
Bsc4I	CCNNNNNNNGG	2 cut(s)	135, 213
Bse118I	RCCGGY	2 cut(s)	128, 192
BseBI	CCWGG	2 cut(s)	65, 214
BseGI	GGATG	1 cut(s)	160
BseLI	CCNNNNNNNGG	2 cut(s)	135, 213
BseMII	CTCAG	2 cut(s)	227, 314
BseRI	GAGGAG	6 cut(s)	4, 7, 10, 13, 173, 253
BseXI	GCAGC	2 cut(s)	156, 329
BseYI	CCCAGC	1 cut(s)	117
Bsh1236I	CGCG	4 cut(s)	262, 267, 319, 321
BshFI	GGCC	2 cut(s)	68, 305
BshNI	GGYRCC	1 cut(s)	269
BsiHKAI	GWGCWC	1 cut(s)	301
BsiSI	CCGG	3 cut(s)	129, 193, 294
BslFI	GGGAC	2 cut(s)	236, 276
BslI	CCNNNNNNNGG	2 cut(s)	135, 213
BsmFI	GGGAC	2 cut(s)	236, 276
BsnI	GGCC	2 cut(s)	68, 305
Bsp1286I	GDGCHC	2 cut(s)	125, 301
Bsp143I	GATC	1 cut(s)	186
Bsp68I	TCGCGA	1 cut(s)	262
BspACI	CCGC	9 cut(s)	69, 87, 90, 102, 126, 132, 267, 321, 331
BspANI	GGCC	2 cut(s)	68, 305
BspCNI	CTCAG	2 cut(s)	228, 313
BspFNI	CGCG	4 cut(s)	262, 267, 319, 321
BspLI	GGNNCC	2 cut(s)	124, 271
BspMAI	CTGCAG	2 cut(s)	174, 347
BspT107I	GGYRCC	1 cut(s)	269
BsrFI	RCCGGY	2 cut(s)	128, 192
BssAI	RCCGGY	2 cut(s)	128, 192
BssMI	GATC	1 cut(s)	186
BssNI	GRCGYC	1 cut(s)	270
Bst2UI	CCWGG	2 cut(s)	65, 214
Bst4CI	ACNGT	2 cut(s)	230, 250
Bst6I	CTCTTC	1 cut(s)	41
BstACI	GRCGYC	1 cut(s)	270
BstC8I	GCNNGC	4 cut(s)	7, 130, 260, 347
BstDEI	CTNAG	3 cut(s)	202, 236, 300
BstF5I	GGATG	1 cut(s)	160
BstFNI	CGCG	4 cut(s)	262, 267, 319, 321
BstH2I	RGCGCY	1 cut(s)	273
BstHHI	GCGC	4 cut(s)	198, 235, 272, 321
BstKTI	GATC	1 cut(s)	189
BstMBI	GATC	1 cut(s)	186
BstMWI	GCNNNNNNNGC	6 cut(s)	129, 169, 264, 327, 330, 339
BstNI	CCWGG	2 cut(s)	65, 214
BstSCI	CCNGG	3 cut(s)	63, 212, 292
BstSFI	CTRYAG	3 cut(s)	170, 325, 343
BstUI	CGCG	4 cut(s)	262, 267, 319, 321
BstV1I	GCAGC	2 cut(s)	156, 329
BsuRI	GGCC	2 cut(s)	68, 305
BtsCI	GGATG	1 cut(s)	160
BtsI	GCAGTG	1 cut(s)	26
BtsIMutI	CAGTG	2 cut(s)	26, 246
BtuMI	TCGCGA	1 cut(s)	262
Cac8I	GCNNGC	4 cut(s)	7, 130, 260, 347
CfoI	GCGC	4 cut(s)	198, 235, 272, 321
Cfr10I	RCCGGY	2 cut(s)	128, 192
CseI	GACGC	1 cut(s)	273
CsiI	ACCWGGT	1 cut(s)	212
Csp6I	GTAC	2 cut(s)	217, 277
CviQI	GTAC	2 cut(s)	217, 277
DdeI	CTNAG	3 cut(s)	202, 236, 300
DinI	GGCGCC	1 cut(s)	271
DpnI	GATC	1 cut(s)	188
DpnII	GATC	1 cut(s)	186
EaeI	YGGCCR	1 cut(s)	66
Eam1104I	CTCTTC	1 cut(s)	41
EarI	CTCTTC	1 cut(s)	41
EciI	GGCGGA	3 cut(s)	91, 115, 147
Ecl136II	GAGCTC	1 cut(s)	299
Eco24I	GRGCYC	2 cut(s)	125, 301
Eco53kI	GAGCTC	1 cut(s)	299
EcoICRI	GAGCTC	1 cut(s)	299
EcoRII	CCWGG	2 cut(s)	63, 212
EcoT38I	GRGCYC	2 cut(s)	125, 301
EgeI	GGCGCC	1 cut(s)	271
EheI	GGCGCC	1 cut(s)	271
FaqI	GGGAC	2 cut(s)	236, 276
Fnu4HI	GCNGC	8 cut(s)	69, 87, 90, 170, 268, 322, 331, 343
FokI	GGATG	1 cut(s)	167
FriOI	GRGCYC	2 cut(s)	125, 301
Fsp4HI	GCNGC	8 cut(s)	69, 87, 90, 170, 268, 322, 331, 343
GlaI	GCGC	4 cut(s)	197, 234, 271, 320
GluI	GCNGC	8 cut(s)	69, 87, 90, 170, 268, 322, 331, 343
GsaI	CCCAGC	1 cut(s)	121
GsuI	CTGGAG	1 cut(s)	185
HaeII	RGCGCY	1 cut(s)	273
HaeIII	GGCC	2 cut(s)	68, 305
HapII	CCGG	3 cut(s)	129, 193, 294
HgaI	GACGC	1 cut(s)	273
HhaI	GCGC	4 cut(s)	198, 235, 272, 321
Hin1I	GRCGYC	1 cut(s)	270
Hin6I	GCGC	4 cut(s)	196, 233, 270, 319
HinP1I	GCGC	4 cut(s)	196, 233, 270, 319
HinfI	GANTC	1 cut(s)	179
HpaII	CCGG	3 cut(s)	129, 193, 294
HphI	GGTGA	1 cut(s)	158
Hpy188I	TCNGA	1 cut(s)	184
Hpy188III	TCNNGA	1 cut(s)	261
Hpy99I	CGWCG	2 cut(s)	110, 267
HpyCH4III	ACNGT	2 cut(s)	230, 250
HpyCH4V	TGCA	2 cut(s)	172, 345
HpyF10VI	GCNNNNNNNGC	6 cut(s)	129, 169, 264, 327, 330, 339
HpyF3I	CTNAG	3 cut(s)	202, 236, 300
Hsp92I	GRCGYC	1 cut(s)	270
HspAI	GCGC	4 cut(s)	196, 233, 270, 319
KasI	GGCGCC	1 cut(s)	269
KroI	GCCGGC	1 cut(s)	128
KroNI	GCCGGC	1 cut(s)	130
Kzo9I	GATC	1 cut(s)	186
LmnI	GCTCC	5 cut(s)	14, 128, 160, 166, 296
Lsp1109I	GCAGC	2 cut(s)	156, 329
LweI	GCATC	2 cut(s)	207, 342
MabI	ACCWGGT	1 cut(s)	212
MalI	GATC	1 cut(s)	188
MboI	GATC	1 cut(s)	186
MboII	GAAGA	2 cut(s)	28, 40
MfeI	CAATTG	1 cut(s)	283
MhlI	GDGCHC	2 cut(s)	125, 301
MluCI	AATT	1 cut(s)	283
Mly113I	GGCGCC	1 cut(s)	270
MlyI	GAGTC	1 cut(s)	173
MreI	CGCCGGCG	1 cut(s)	128
MroNI	GCCGGC	1 cut(s)	128
MspA1I	CMGCKG	1 cut(s)	117
MspI	CCGG	3 cut(s)	129, 193, 294
MspR9I	CCNGG	3 cut(s)	65, 214, 294
MunI	CAATTG	1 cut(s)	283
MvaI	CCWGG	2 cut(s)	65, 214
MvnI	CGCG	4 cut(s)	262, 267, 319, 321
MwoI	GCNNNNNNNGC	6 cut(s)	129, 169, 264, 327, 330, 339
NaeI	GCCGGC	1 cut(s)	130
NarI	GGCGCC	1 cut(s)	270
NciI	CCSGG	1 cut(s)	294
NdeII	GATC	1 cut(s)	186
NgoMIV	GCCGGC	1 cut(s)	128
NlaIV	GGNNCC	2 cut(s)	124, 271
NruI	TCGCGA	1 cut(s)	262
PdiI	GCCGGC	1 cut(s)	130
PfoI	TCCNGGA	1 cut(s)	292
PkrI	GCNGC	8 cut(s)	70, 88, 91, 171, 269, 323, 332, 344
PleI	GAGTC	1 cut(s)	173
PluTI	GGCGCC	1 cut(s)	273
PpsI	GAGTC	1 cut(s)	173
Psp124BI	GAGCTC	1 cut(s)	301
Psp6I	CCWGG	2 cut(s)	63, 212
PspFI	CCCAGC	1 cut(s)	117
PspGI	CCWGG	2 cut(s)	63, 212
PspN4I	GGNNCC	2 cut(s)	124, 271
PstI	CTGCAG	2 cut(s)	174, 347
PvuII	CAGCTG	1 cut(s)	117
RruI	TCGCGA	1 cut(s)	262
RsaI	GTAC	2 cut(s)	218, 278
RsaNI	GTAC	2 cut(s)	217, 277
SacI	GAGCTC	1 cut(s)	301
SatI	GCNGC	8 cut(s)	69, 87, 90, 170, 268, 322, 331, 343
Sau3AI	GATC	1 cut(s)	186
SchI	GAGTC	1 cut(s)	173
ScrFI	CCNGG	3 cut(s)	65, 214, 294
SduI	GDGCHC	2 cut(s)	125, 301
SetI	ASST	8 cut(s)	11, 119, 148, 165, 171, 218, 227, 301
SexAI	ACCWGGT	1 cut(s)	212
SfaNI	GCATC	2 cut(s)	207, 342
SfcI	CTRYAG	3 cut(s)	170, 325, 343
SfoI	GGCGCC	1 cut(s)	271
SgrAI	CRCCGGYG	1 cut(s)	128
Sse9I	AATT	1 cut(s)	283
SsiI	CCGC	9 cut(s)	69, 87, 90, 102, 126, 132, 267, 321, 331
SspDI	GGCGCC	1 cut(s)	269
SstI	GAGCTC	1 cut(s)	301
StyD4I	CCNGG	3 cut(s)	63, 212, 292
TaaI	ACNGT	2 cut(s)	230, 250
TaqI	TCGA	1 cut(s)	108
TasI	AATT	1 cut(s)	283
TauI	GCSGC	6 cut(s)	71, 89, 92, 270, 324, 333
TscAI	CASTG	2 cut(s)	33, 253
TseI	GCWGC	2 cut(s)	169, 342
TspDTI	ATGAA	1 cut(s)	34
TspRI	CASTG	2 cut(s)	33, 253

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.