Rroxscaffold_6G00424110

rapid alkalinization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004612
Physical Location & Seq
Reverse (-)
45327651 .. 45328866
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424110.1

Sequence Viewer

Length: 330 bp
ATGGCCAAGCTACTATACTCTTTAGCCATCCTCCTGATCTGGGCCACAATCTTTCTCATGGCTTCATCTTCCACCGTTGAAGCAAGTGGAGACCTTCCATGGATACCCACAACCGCCACCGGCTTAGTCTCCGGGGACGATGAGTTCGACCTTGACTCGGAGATCAACCGGCGCATCTTAGCCACCAGCGGCTACATCAGCTACGGTGCACTGCAGAGGAACACTGTCCCCTGCTCCAGGCGCGGCGCCTCCTACTACAATTGCCAGCCTGGGGCTCAGGCCAACCCTTATAGCCGCGGCTGCAGCGCCATCACAAGGTGCAGGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

109

Amino Acids

11.79

Weight (kDa)

7.63

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 48 - 108 4.2e-29 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 245
AccII CGCG 2 cut(s) 243, 297
AciI CCGC 5 cut(s) 114, 189, 243, 295, 297
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 246
AdeI CACNNNGTG 1 cut(s) 318
AfiI CCNNNNNNNGG 5 cut(s) 40, 157, 237, 271, 315
AgsI TTSAA 1 cut(s) 80
AjnI CCWGG 2 cut(s) 236, 268
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
AluBI AGCT 2 cut(s) 10, 201
AluI AGCT 2 cut(s) 10, 201
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 2 cut(s) 84, 133
Alw44I GTGCAC 1 cut(s) 207
AoxI GGCC 3 cut(s) 3, 42, 279
ApaLI GTGCAC 1 cut(s) 207
ApeKI GCWGC 2 cut(s) 300, 303
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 4 cut(s) 174, 243, 248, 308
AspS9I GGNCC 1 cut(s) 42
AsuC2I CCSGG 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 211
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 2 cut(s) 287, 315
BccI CCATC 2 cut(s) 35, 317
BciT130I CCWGG 2 cut(s) 238, 270
BciVI GTATCC 1 cut(s) 96
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 84, 133
BfmI CTRYAG 2 cut(s) 212, 301
BfoI RGCGCY 2 cut(s) 249, 309
BfuI GTATCC 1 cut(s) 96
BisI GCNGC 6 cut(s) 190, 244, 295, 298, 301, 304
BlsI GCNGC 6 cut(s) 191, 245, 296, 299, 302, 305
Bme1390I CCNGG 3 cut(s) 133, 238, 270
BmgT120I GGNCC 1 cut(s) 42
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 3 cut(s) 133, 238, 270
BmsI GCATC 1 cut(s) 183
BpmI CTGGAG 1 cut(s) 220
Bpu10I CCTNAGC 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 133
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 4 cut(s) 98, 132, 269, 295
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 157, 237, 271, 315
Bse118I RCCGGY 2 cut(s) 119, 168
BseBI CCWGG 2 cut(s) 238, 270
BseDI CCNNGG 4 cut(s) 98, 132, 269, 295
BseGI GGATG 1 cut(s) 27
BseLI CCNNNNNNNGG 5 cut(s) 40, 157, 237, 271, 315
BseMII CTCAG 1 cut(s) 290
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 2 cut(s) 287, 315
Bsh1236I CGCG 2 cut(s) 243, 297
BshFI GGCC 3 cut(s) 5, 44, 281
BshNI GGYRCC 1 cut(s) 245
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 3 cut(s) 120, 132, 169
BslFI GGGAC 2 cut(s) 149, 212
BslI CCNNNNNNNGG 5 cut(s) 40, 157, 237, 271, 315
BsmAI GTCTC 2 cut(s) 84, 133
BsmFI GGGAC 2 cut(s) 149, 212
BsnI GGCC 3 cut(s) 5, 44, 281
Bso31I GGTCTC 1 cut(s) 84
Bsp1286I GDGCHC 2 cut(s) 211, 277
Bsp143I GATC 2 cut(s) 36, 162
Bsp19I CCATGG 1 cut(s) 98
BspACI CCGC 5 cut(s) 114, 189, 243, 295, 297
BspANI GGCC 3 cut(s) 5, 44, 281
BspCNI CTCAG 1 cut(s) 289
BspFNI CGCG 2 cut(s) 243, 297
BspLI GGNNCC 1 cut(s) 247
BspMAI CTGCAG 2 cut(s) 216, 305
BspT107I GGYRCC 1 cut(s) 245
BspTNI GGTCTC 1 cut(s) 84
BsrFI RCCGGY 2 cut(s) 119, 168
BssAI RCCGGY 2 cut(s) 119, 168
BssECI CCNNGG 4 cut(s) 98, 132, 269, 295
BssMI GATC 2 cut(s) 36, 162
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 98
Bst2UI CCWGG 2 cut(s) 238, 270
Bst4CI ACNGT 3 cut(s) 76, 206, 226
BstACI GRCGYC 1 cut(s) 246
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 3 cut(s) 124, 178, 276
BstDSI CCRYGG 2 cut(s) 98, 295
BstENI CCTNNNNNAGG 1 cut(s) 235
BstF5I GGATG 1 cut(s) 27
BstFNI CGCG 2 cut(s) 243, 297
BstH2I RGCGCY 2 cut(s) 249, 309
BstHHI GCGC 4 cut(s) 174, 243, 248, 308
BstKTI GATC 2 cut(s) 39, 165
BstMAI GTCTC 2 cut(s) 84, 133
BstMBI GATC 2 cut(s) 36, 162
BstMWI GCNNNNNNNGC 4 cut(s) 198, 240, 300, 303
BstNI CCWGG 2 cut(s) 238, 270
BstSCI CCNGG 3 cut(s) 131, 236, 268
BstSFI CTRYAG 2 cut(s) 212, 301
BstSLI GKGCMC 1 cut(s) 211
BstUI CGCG 2 cut(s) 243, 297
BstV1I GCAGC 2 cut(s) 287, 315
BsuI GTATCC 1 cut(s) 96
BsuRI GGCC 3 cut(s) 5, 44, 281
BtgI CCRYGG 2 cut(s) 98, 295
BtsCI GGATG 1 cut(s) 27
BtsI GCAGTG 1 cut(s) 209
BtsIMutI CAGTG 2 cut(s) 209, 222
Cac8I GCNNGC 1 cut(s) 266
CfoI GCGC 4 cut(s) 174, 243, 248, 308
Cfr10I RCCGGY 2 cut(s) 119, 168
Cfr13I GGNCC 1 cut(s) 42
Cfr42I CCGCGG 1 cut(s) 298
CviAII CATG 2 cut(s) 58, 99
DdeI CTNAG 3 cut(s) 124, 178, 276
DinI GGCGCC 1 cut(s) 247
DpnI GATC 2 cut(s) 38, 164
DpnII GATC 2 cut(s) 36, 162
DraIII CACNNNGTG 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 277
Eco31I GGTCTC 1 cut(s) 84
EcoNI CCTNNNNNAGG 1 cut(s) 235
EcoRII CCWGG 2 cut(s) 236, 268
EcoT14I CCWWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 277
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 2 cut(s) 61, 102
FaiI YATR 4 cut(s) 16, 59, 100, 291
FaqI GGGAC 2 cut(s) 149, 212
FatI CATG 2 cut(s) 57, 98
Fnu4HI GCNGC 6 cut(s) 190, 244, 295, 298, 301, 304
FokI GGATG 1 cut(s) 14
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 6 cut(s) 190, 244, 295, 298, 301, 304
GlaI GCGC 4 cut(s) 173, 242, 247, 307
GluI GCNGC 6 cut(s) 190, 244, 295, 298, 301, 304
GsuI CTGGAG 1 cut(s) 220
HaeII RGCGCY 2 cut(s) 249, 309
HaeIII GGCC 3 cut(s) 5, 44, 281
HapII CCGG 3 cut(s) 120, 132, 169
HhaI GCGC 4 cut(s) 174, 243, 248, 308
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 2 cut(s) 61, 102
Hin6I GCGC 4 cut(s) 172, 241, 246, 306
HinP1I GCGC 4 cut(s) 172, 241, 246, 306
HinfI GANTC 1 cut(s) 155
HpaII CCGG 3 cut(s) 120, 132, 169
Hpy166II GTNNAC 1 cut(s) 209
Hpy188I TCNGA 1 cut(s) 160
Hpy188III TCNNGA 1 cut(s) 34
Hpy8I GTNNAC 1 cut(s) 209
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 3 cut(s) 76, 206, 226
HpyCH4V TGCA 4 cut(s) 209, 214, 303, 321
HpyF10VI GCNNNNNNNGC 4 cut(s) 198, 240, 300, 303
HpyF3I CTNAG 3 cut(s) 124, 178, 276
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 2 cut(s) 61, 102
HspAI GCGC 4 cut(s) 172, 241, 246, 306
KasI GGCGCC 1 cut(s) 245
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 2 cut(s) 36, 162
LmnI GCTCC 1 cut(s) 239
Lsp1109I GCAGC 2 cut(s) 287, 315
LweI GCATC 1 cut(s) 183
MalI GATC 2 cut(s) 38, 164
MboI GATC 2 cut(s) 36, 162
MboII GAAGA 1 cut(s) 60
MfeI CAATTG 1 cut(s) 259
MhlI GDGCHC 2 cut(s) 211, 277
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 259
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 246
MlyI GAGTC 1 cut(s) 149
MnlI CCTC 3 cut(s) 41, 210, 259
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 328
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 189, 297
MspI CCGG 3 cut(s) 120, 132, 169
MspR9I CCNGG 3 cut(s) 133, 238, 270
MteI GCGCNGCGC 1 cut(s) 244
MunI CAATTG 1 cut(s) 259
MvaI CCWGG 2 cut(s) 238, 270
MvnI CGCG 2 cut(s) 243, 297
MwoI GCNNNNNNNGC 4 cut(s) 198, 240, 300, 303
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 1 cut(s) 133
NcoI CCATGG 1 cut(s) 98
NdeII GATC 2 cut(s) 36, 162
NlaIII CATG 2 cut(s) 61, 102
NlaIV GGNNCC 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PkrI GCNGC 6 cut(s) 191, 245, 296, 299, 302, 305
PleI GAGTC 1 cut(s) 149
PluTI GGCGCC 1 cut(s) 249
PpsI GAGTC 1 cut(s) 149
Psp6I CCWGG 2 cut(s) 236, 268
PspGI CCWGG 2 cut(s) 236, 268
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 1 cut(s) 42
PstI CTGCAG 2 cut(s) 216, 305
SacII CCGCGG 1 cut(s) 298
SaqAI TTAA 1 cut(s) 328
SatI GCNGC 6 cut(s) 190, 244, 295, 298, 301, 304
Sau3AI GATC 2 cut(s) 36, 162
Sau96I GGNCC 1 cut(s) 42
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 3 cut(s) 133, 238, 270
SduI GDGCHC 2 cut(s) 211, 277
SetI ASST 5 cut(s) 12, 96, 153, 203, 320
SfaNI GCATC 1 cut(s) 183
SfcI CTRYAG 2 cut(s) 212, 301
SfoI GGCGCC 1 cut(s) 247
Sfr303I CCGCGG 1 cut(s) 298
SgrBI CCGCGG 1 cut(s) 298
Sse9I AATT 1 cut(s) 259
SsiI CCGC 5 cut(s) 114, 189, 243, 295, 297
SspDI GGCGCC 1 cut(s) 245
StyD4I CCNGG 3 cut(s) 131, 236, 268
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 3 cut(s) 76, 206, 226
TaqI TCGA 1 cut(s) 147
TasI AATT 1 cut(s) 259
TauI GCSGC 4 cut(s) 192, 246, 297, 300
Tru1I TTAA 1 cut(s) 328
Tru9I TTAA 1 cut(s) 328
TscAI CASTG 2 cut(s) 216, 229
TseI GCWGC 2 cut(s) 300, 303
TspDTI ATGAA 1 cut(s) 54
TspRI CASTG 2 cut(s) 216, 229
VneI GTGCAC 1 cut(s) 207
XagI CCTNNNNNAGG 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.