FvH4_4g27320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003758
Physical Location & Seq
Forward (+)
28365537 .. 28366554
1018 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27320.t1

Sequence Viewer

Length: 516 bp
ATGAATCACTGTGCGATTCAGCAGAACAACGCGTGCGAAGAGATGAGGAATTTGAGAGACGCCGTCGTTTGTCCCAAGCCTCGCCGTTTGAGGAACGCTGTCCTTCACGAGCCCGTCAGATCTTGTCGATGGCTTCTAGGCCACCAAGCTGAGCTGAGTGATGGGAGAGCAGGGTCAGATCTTTTGGAAAATCTCCTTACCAAGGGTTCTTATGGTGTTGAACAATTTTACTGTACAGAAATAGCGTCGGCGCACCCATTTTATAGCGGGTCACCACCGAGCAGAGTAGCTAACCCACTAATTCAGGATGCTAGATTTGGGGATGAGAAGGTCATCTCATCATCACCACTGTCACCTACCCCTCCCCCTTCAGGTCTGTCATTGTCTCCATCATCAACTGCAAGGAAAGTAGGTTGTGTTCGGGCCAATTTTGGAAGCAAGCCAGCAGTGAGGGTTGAGGGGTTTGATTGCCTTGATAGGGATCGGAGAAACTGTAGAATCCCTACCCTGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

18.73

Weight (kDa)

8.88

Isoelectric Point (pI)

66.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 32
AciI CCGC 1 cut(s) 267
AclWI GGATC 1 cut(s) 489
AcsI RAATTY 1 cut(s) 49
AcuI CTGAAG 1 cut(s) 354
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 4 cut(s) 202, 371, 478, 508
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 1 cut(s) 221
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 3 cut(s) 149, 154, 290
AluI AGCT 3 cut(s) 149, 154, 290
Alw26I GTCTC 2 cut(s) 51, 390
AlwI GGATC 1 cut(s) 489
AoxI GGCC 2 cut(s) 139, 423
ApoI RAATTY 1 cut(s) 49
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 1 cut(s) 423
AsuHPI GGTGA 3 cut(s) 264, 336, 345
BanII GRGCYC 1 cut(s) 114
BauI CACGAG 1 cut(s) 107
BccI CCATC 3 cut(s) 123, 155, 397
BceAI ACGGC 2 cut(s) 47, 69
BciT130I CCWGG 1 cut(s) 509
BcoDI GTCTC 2 cut(s) 51, 390
BfaI CTAG 2 cut(s) 137, 312
BfmI CTRYAG 1 cut(s) 493
BglII AGATCT 2 cut(s) 119, 178
BlpI GCTNAGC 1 cut(s) 150
Bme1390I CCNGG 1 cut(s) 509
BmgT120I GGNCC 1 cut(s) 423
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 1 cut(s) 298
Bpu1102I GCTNAGC 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 480
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 201, 507
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 371, 478, 508
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 2 cut(s) 201, 507
BseGI GGATG 2 cut(s) 313, 328
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 4 cut(s) 202, 371, 478, 508
BseMII CTCAG 2 cut(s) 141, 146
Bsh1236I CGCG 1 cut(s) 32
BshFI GGCC 2 cut(s) 141, 425
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 4 cut(s) 202, 371, 478, 508
BsmAI GTCTC 2 cut(s) 51, 390
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 57
BsnI GGCC 2 cut(s) 141, 425
Bsp1286I GDGCHC 1 cut(s) 114
Bsp1407I TGTACA 1 cut(s) 233
Bsp143I GATC 3 cut(s) 119, 178, 481
Bsp1720I GCTNAGC 1 cut(s) 150
BspACI CCGC 1 cut(s) 267
BspANI GGCC 2 cut(s) 141, 425
BspCNI CTCAG 2 cut(s) 142, 147
BspFNI CGCG 1 cut(s) 32
BspPI GGATC 1 cut(s) 489
BsrGI TGTACA 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 201, 507
BssMI GATC 3 cut(s) 119, 178, 481
BssNI GRCGYC 1 cut(s) 60
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 201
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 4 cut(s) 11, 233, 351, 494
Bst6I CTCTTC 1 cut(s) 33
BstACI GRCGYC 1 cut(s) 60
BstAUI TGTACA 1 cut(s) 233
BstC8I GCNNGC 3 cut(s) 34, 440, 444
BstDEI CTNAG 2 cut(s) 150, 155
BstEII GGTNACC 1 cut(s) 270
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 2 cut(s) 313, 328
BstFNI CGCG 1 cut(s) 32
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 3 cut(s) 122, 181, 484
BstMAI GTCTC 2 cut(s) 51, 390
BstMBI GATC 3 cut(s) 119, 178, 481
BstNI CCWGG 1 cut(s) 509
BstPI GGTNACC 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 507
BstSFI CTRYAG 1 cut(s) 493
BstUI CGCG 1 cut(s) 32
BstX2I RGATCY 2 cut(s) 119, 178
BstYI RGATCY 2 cut(s) 119, 178
BsuRI GGCC 2 cut(s) 141, 425
BtsCI GGATG 2 cut(s) 313, 328
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 3 cut(s) 7, 347, 453
Cac8I GCNNGC 3 cut(s) 34, 440, 444
CfoI GCGC 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 423
CseI GACGC 2 cut(s) 68, 234
Csp6I GTAC 1 cut(s) 234
CviJI RGCY 9 cut(s) 79, 112, 133, 141, 149, 154, 290, 425, 442
CviKI_1 RGCY 9 cut(s) 79, 112, 133, 141, 149, 154, 290, 425, 442
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 2 cut(s) 150, 155
DpnI GATC 3 cut(s) 121, 180, 483
DpnII GATC 3 cut(s) 119, 178, 481
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 354
Eco91I GGTNACC 1 cut(s) 270
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO65I GGTNACC 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 507
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 1 cut(s) 51
FaiI YATR 3 cut(s) 213, 264, 514
FaqI GGGAC 1 cut(s) 57
FauI CCCGC 1 cut(s) 260
FokI GGATG 2 cut(s) 320, 335
FriOI GRGCYC 1 cut(s) 114
FspBI CTAG 2 cut(s) 137, 312
GlaI GCGC 1 cut(s) 252
HaeIII GGCC 2 cut(s) 141, 425
HgaI GACGC 2 cut(s) 68, 234
HhaI GCGC 1 cut(s) 253
Hin1I GRCGYC 1 cut(s) 60
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HinfI GANTC 3 cut(s) 4, 16, 498
HphI GGTGA 3 cut(s) 264, 336, 345
Hpy188I TCNGA 3 cut(s) 119, 178, 486
Hpy188III TCNNGA 2 cut(s) 107, 305
Hpy99I CGWCG 2 cut(s) 68, 250
HpyAV CCTTC 3 cut(s) 113, 322, 378
HpyCH4III ACNGT 4 cut(s) 11, 233, 351, 494
HpyCH4V TGCA 1 cut(s) 401
HpyF3I CTNAG 2 cut(s) 150, 155
Hsp92I GRCGYC 1 cut(s) 60
HspAI GCGC 1 cut(s) 251
Kzo9I GATC 3 cut(s) 119, 178, 481
LpnPI CCDG 5 cut(s) 156, 290, 357, 456, 494
LweI GCATC 1 cut(s) 298
MaeI CTAG 2 cut(s) 137, 312
MaeIII GTNAC 2 cut(s) 270, 351
MalI GATC 3 cut(s) 121, 180, 483
MboI GATC 3 cut(s) 119, 178, 481
MboII GAAGA 1 cut(s) 50
MflI RGATCY 2 cut(s) 119, 178
MhlI GDGCHC 1 cut(s) 114
MluCI AATT 4 cut(s) 49, 224, 300, 427
MluI ACGCGT 1 cut(s) 30
MnlI CCTC 6 cut(s) 39, 84, 90, 372, 444, 451
MspR9I CCNGG 1 cut(s) 509
MvaI CCWGG 1 cut(s) 509
MvnI CGCG 1 cut(s) 32
NdeII GATC 3 cut(s) 119, 178, 481
NmuCI GTSAC 2 cut(s) 270, 351
PfeI GAWTC 3 cut(s) 4, 16, 498
PflFI GACNNNGTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 507
PspEI GGTNACC 1 cut(s) 270
PspGI CCWGG 1 cut(s) 507
PspPI GGNCC 1 cut(s) 423
PsrI GAACNNNNNNTAC 2 cut(s) 402, 434
PsuI RGATCY 2 cut(s) 119, 178
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
Sau3AI GATC 3 cut(s) 119, 178, 481
Sau96I GGNCC 1 cut(s) 423
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 1 cut(s) 114
SetI ASST 7 cut(s) 151, 156, 292, 333, 358, 376, 415
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 1 cut(s) 493
Sse9I AATT 4 cut(s) 49, 224, 300, 427
SsiI CCGC 1 cut(s) 267
SspMI CTAG 2 cut(s) 137, 312
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 4 cut(s) 11, 233, 351, 494
TaqI TCGA 1 cut(s) 127
TasI AATT 4 cut(s) 49, 224, 300, 427
TatI WGTACW 1 cut(s) 233
TfiI GAWTC 3 cut(s) 4, 16, 498
TscAI CASTG 3 cut(s) 14, 354, 453
TseFI GTSAC 2 cut(s) 270, 351
Tsp45I GTSAC 2 cut(s) 270, 351
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 14, 354, 453
Tth111I GACNNNGTC 1 cut(s) 62
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 1 cut(s) 49
XspI CTAG 2 cut(s) 137, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.