Rroxscaffold_5G00376340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003758
Physical Location & Seq
Forward (+)
57041384 .. 57042878
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00376340.1

Sequence Viewer

Length: 510 bp
ATGAACCACTGCGTGATACAGCAGAGCAGCGCCCTTTCGGTTTGTGAAGAGATGAGGACGGCGAGAGACTCCGTCGTTTGCCCCAAACCGCGCCGTTTCGGACTCCGAAACGCCACCTTTCACGATCCCGGCAGATCTCTCAGATGGTATCTCGGCCATCAAGGGGAGCTCTGTGATTCAAAGGCAGGCTCTGATGCTTTGGACTGTATCTTAACAAAGGGGGTTGAACAGTTTTCTTGTACAGAGATAGGCTCGCCGCACCCATTTTTCAGCGGATCGCCGCCGAGCAGAGTAGCTAATCCATTGATTCGGGATGCTAGATTTGGCAGTGAGAAGCTCACTCCAGTGTCACCGCTTTCACCGGTGTCATCATCGTCTCCATCATCTTCCACAAGGAATGGAGGAGGCTGTGTTCGTGCTATTTTCGGGAGTAAACCAACAGTGAGGGTCGAGGGGTTTGATTGCCTGGACAGGGATAGCCGAAATTGCAGCATCCCTTCCCTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.19

Weight (kDa)

8.65

Isoelectric Point (pI)

72.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 91
AciI CCGC 5 cut(s) 89, 257, 273, 281, 353
AclWI GGATC 2 cut(s) 119, 283
AcoI YGGCCR 1 cut(s) 154
AdeI CACNNNGTG 1 cut(s) 13
AfaI GTAC 1 cut(s) 241
AfiI CCNNNNNNNGG 3 cut(s) 163, 472, 502
AgeI ACCGGT 1 cut(s) 361
AgsI TTSAA 2 cut(s) 180, 227
AjnI CCWGG 2 cut(s) 465, 501
AleI CACNNNNGTG 1 cut(s) 344
AloI GAACNNNNNNTCC 2 cut(s) 396, 428
AluBI AGCT 3 cut(s) 169, 296, 337
AluI AGCT 3 cut(s) 169, 296, 337
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 2 cut(s) 60, 381
AlwI GGATC 2 cut(s) 119, 283
AlwNI CAGNNNCTG 1 cut(s) 191
AoxI GGCC 1 cut(s) 154
ApeKI GCWGC 2 cut(s) 27, 489
AsiGI ACCGGT 1 cut(s) 361
AspLEI GCGC 2 cut(s) 32, 93
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 2 cut(s) 342, 351
BanII GRGCYC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 39, 501
BccI CCATC 3 cut(s) 138, 165, 388
BceAI ACGGC 2 cut(s) 75, 78
BciT130I CCWGG 2 cut(s) 467, 503
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 2 cut(s) 60, 381
BfaI CTAG 1 cut(s) 318
BfoI RGCGCY 1 cut(s) 33
BglII AGATCT 1 cut(s) 134
BisI GCNGC 4 cut(s) 28, 257, 281, 490
BlsI GCNGC 4 cut(s) 29, 258, 282, 491
Bme1390I CCNGG 3 cut(s) 129, 467, 503
BmrFI CCNGG 3 cut(s) 129, 467, 503
BmsI GCATC 3 cut(s) 184, 304, 501
BplI GAGNNNNNCTC 2 cut(s) 236, 268
BpmI CTGGAG 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 501
BsaWI WCCGGW 1 cut(s) 361
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 472, 502
Bse118I RCCGGY 1 cut(s) 361
Bse1I ACTGG 1 cut(s) 344
BseBI CCWGG 2 cut(s) 467, 503
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 2 cut(s) 319, 492
BseLI CCNNNNNNNGG 3 cut(s) 163, 472, 502
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 1 cut(s) 344
BseRI GAGGAG 1 cut(s) 417
BseXI GCAGC 2 cut(s) 39, 501
Bsh1236I CGCG 1 cut(s) 91
BshFI GGCC 1 cut(s) 156
BshTI ACCGGT 1 cut(s) 361
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 2 cut(s) 129, 362
BslI CCNNNNNNNGG 3 cut(s) 163, 472, 502
BsmAI GTCTC 2 cut(s) 60, 381
BsmBI CGTCTC 1 cut(s) 381
BsnI GGCC 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 171
Bsp1407I TGTACA 1 cut(s) 239
Bsp143I GATC 3 cut(s) 124, 134, 275
BspACI CCGC 5 cut(s) 89, 257, 273, 281, 353
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 1 cut(s) 153
BspFNI CGCG 1 cut(s) 91
BspPI GGATC 2 cut(s) 119, 283
BsrFI RCCGGY 1 cut(s) 361
BsrGI TGTACA 1 cut(s) 239
BsrI ACTGG 1 cut(s) 344
BssAI RCCGGY 1 cut(s) 361
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 3 cut(s) 124, 134, 275
Bst2UI CCWGG 2 cut(s) 467, 503
Bst4CI ACNGT 3 cut(s) 206, 231, 442
Bst6I CTCTTC 1 cut(s) 42
BstAUI TGTACA 1 cut(s) 239
BstC8I GCNNGC 2 cut(s) 187, 254
BstDEI CTNAG 1 cut(s) 140
BstF5I GGATG 2 cut(s) 319, 492
BstFNI CGCG 1 cut(s) 91
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 2 cut(s) 32, 93
BstKTI GATC 3 cut(s) 127, 137, 278
BstMAI GTCTC 2 cut(s) 60, 381
BstMBI GATC 3 cut(s) 124, 134, 275
BstMWI GCNNNNNNNGC 1 cut(s) 486
BstNI CCWGG 2 cut(s) 467, 503
BstSCI CCNGG 3 cut(s) 127, 465, 501
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 2 cut(s) 39, 501
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
BsuRI GGCC 1 cut(s) 156
BtsCI GGATG 2 cut(s) 319, 492
BtsI GCAGTG 2 cut(s) 7, 334
BtsIMutI CAGTG 4 cut(s) 7, 334, 351, 447
Cac8I GCNNGC 2 cut(s) 187, 254
CaiI CAGNNNCTG 1 cut(s) 191
CfoI GCGC 2 cut(s) 32, 93
Cfr10I RCCGGY 1 cut(s) 361
Csp6I GTAC 1 cut(s) 240
CspAI ACCGGT 1 cut(s) 361
CviJI RGCY 9 cut(s) 156, 169, 189, 252, 296, 337, 408, 480, 506
CviKI_1 RGCY 9 cut(s) 156, 169, 189, 252, 296, 337, 408, 480, 506
CviQI GTAC 1 cut(s) 240
DdeI CTNAG 1 cut(s) 140
DpnI GATC 3 cut(s) 126, 136, 277
DpnII GATC 3 cut(s) 124, 134, 275
DraIII CACNNNGTG 1 cut(s) 13
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 171
Eco53kI GAGCTC 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 465, 501
EcoT38I GRGCYC 1 cut(s) 171
Esp3I CGTCTC 1 cut(s) 381
Fnu4HI GCNGC 4 cut(s) 28, 257, 281, 490
FokI GGATG 2 cut(s) 326, 479
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 4 cut(s) 28, 257, 281, 490
FspBI CTAG 1 cut(s) 318
GlaI GCGC 2 cut(s) 31, 92
GluI GCNGC 4 cut(s) 28, 257, 281, 490
GsuI CTGGAG 1 cut(s) 327
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 1 cut(s) 156
HapII CCGG 2 cut(s) 129, 362
HhaI GCGC 2 cut(s) 32, 93
Hin6I GCGC 2 cut(s) 30, 91
HinP1I GCGC 2 cut(s) 30, 91
HinfI GANTC 4 cut(s) 68, 102, 176, 307
HpaII CCGG 2 cut(s) 129, 362
HphI GGTGA 2 cut(s) 342, 351
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 4 cut(s) 101, 107, 143, 193
Hpy188III TCNNGA 3 cut(s) 122, 311, 427
Hpy8I GTNNAC 1 cut(s) 434
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 507
HpyCH4III ACNGT 3 cut(s) 206, 231, 442
HpyCH4V TGCA 1 cut(s) 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 486
HpyF3I CTNAG 1 cut(s) 140
HspAI GCGC 2 cut(s) 30, 91
Kzo9I GATC 3 cut(s) 124, 134, 275
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 8 cut(s) 142, 171, 357, 375, 452, 457, 479, 488
Lsp1109I GCAGC 2 cut(s) 39, 501
LweI GCATC 3 cut(s) 184, 304, 501
MaeI CTAG 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 348
MalI GATC 3 cut(s) 126, 136, 277
MboI GATC 3 cut(s) 124, 134, 275
MboII GAAGA 2 cut(s) 59, 378
MflI RGATCY 1 cut(s) 134
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 1 cut(s) 484
MlyI GAGTC 2 cut(s) 62, 96
MnlI CCTC 5 cut(s) 48, 395, 398, 438, 445
MseI TTAA 1 cut(s) 212
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 273
MspI CCGG 2 cut(s) 129, 362
MspR9I CCNGG 3 cut(s) 129, 467, 503
MvaI CCWGG 2 cut(s) 467, 503
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 1 cut(s) 486
NciI CCSGG 1 cut(s) 129
NdeII GATC 3 cut(s) 124, 134, 275
NmeAIII GCCGAG 2 cut(s) 132, 309
NmuCI GTSAC 1 cut(s) 348
OliI CACNNNNGTG 1 cut(s) 344
PfeI GAWTC 2 cut(s) 176, 307
PflFI GACNNNGTC 1 cut(s) 71
PinAI ACCGGT 1 cut(s) 361
PkrI GCNGC 4 cut(s) 29, 258, 282, 491
PleI GAGTC 2 cut(s) 62, 96
PpsI GAGTC 2 cut(s) 62, 96
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 465, 501
PspGI CCWGG 2 cut(s) 465, 501
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 1 cut(s) 134
PsyI GACNNNGTC 1 cut(s) 71
RsaI GTAC 1 cut(s) 241
RsaNI GTAC 1 cut(s) 240
RseI CAYNNNNRTG 1 cut(s) 344
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 4 cut(s) 28, 257, 281, 490
Sau3AI GATC 3 cut(s) 124, 134, 275
SchI GAGTC 2 cut(s) 62, 96
ScrFI CCNGG 3 cut(s) 129, 467, 503
SduI GDGCHC 1 cut(s) 171
SetI ASST 4 cut(s) 119, 171, 298, 339
SfaNI GCATC 3 cut(s) 184, 304, 501
SgrAI CRCCGGYG 1 cut(s) 361
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse9I AATT 1 cut(s) 484
SsiI CCGC 5 cut(s) 89, 257, 273, 281, 353
SspMI CTAG 1 cut(s) 318
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 3 cut(s) 127, 465, 501
TaaI ACNGT 3 cut(s) 206, 231, 442
TaqI TCGA 1 cut(s) 450
TasI AATT 1 cut(s) 484
TatI WGTACW 1 cut(s) 239
TauI GCSGC 2 cut(s) 259, 283
TfiI GAWTC 2 cut(s) 176, 307
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 4 cut(s) 14, 334, 351, 447
TseFI GTSAC 1 cut(s) 348
TseI GCWGC 2 cut(s) 27, 489
Tsp45I GTSAC 1 cut(s) 348
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 4 cut(s) 14, 334, 351, 447
Tth111I GACNNNGTC 1 cut(s) 71
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.