MD16G1042400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003758
Physical Location & Seq
Forward (+)
3002965 .. 3004578
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1042400.v1.1.491

Sequence Viewer

Length: 534 bp
ATGAATCACTGTGCGATTCAGCAAGGAAACGCCCTCTCAGCTTGCGACGACATGAGGACTTGTTCAAGAGACTCGGTCGTTTGTCCCAAACCTCGCCGTTTTGGTCTCCTTAGCGTCACTCACAACGATCCCGTCAGATCTCTCCGATGGCATCTCGGCCACCAAGCGGAGCTCTATGATTCGAAAGCAGGGTCTGATATTTTGGACATCCTCCTCCCAAAGGGATCTTTTGGTGTTGAGCCATTTTCATGTACAGAAATAGCCTCGCCGCACCCATTTTTCTGCGGGTCACCGCCGAGCAGAGTAGCTAACCCATTAATTCATGATGCTAGATTTGGGGATGGGAAACCCACCCCATTGTCGCCACTGTCGCCAACCGCTCCCCCATTGGACCTTTCGTCGTCTCCTTCATCATCAGCAAGGAAAGGAGGCTTCGTTCGGGCCAGTTTCGGGAACAAGCCAGCAGTGAGGGTCGAGGGGTTTGATTGCCTGGACAGGGATCGACGTAATTGCAGCATCCCGACCCTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

19.05

Weight (kDa)

8.48

Isoelectric Point (pI)

72.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 380
AciI CCGC 5 cut(s) 167, 269, 285, 293, 378
AclWI GGATC 3 cut(s) 122, 232, 507
AcoI YGGCCR 1 cut(s) 157
AfaI GTAC 1 cut(s) 253
AfiI CCNNNNNNNGG 5 cut(s) 166, 220, 450, 496, 526
AgsI TTSAA 1 cut(s) 66
AhdI GACNNNNNGTC 1 cut(s) 397
AjnI CCWGG 2 cut(s) 489, 525
AloI GAACNNNNNNTCC 2 cut(s) 420, 452
AluBI AGCT 3 cut(s) 41, 172, 308
AluI AGCT 3 cut(s) 41, 172, 308
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 3 cut(s) 63, 110, 408
AlwI GGATC 3 cut(s) 122, 232, 507
AlwNI CAGNNNCTG 1 cut(s) 194
AoxI GGCC 2 cut(s) 157, 441
ApeKI GCWGC 1 cut(s) 513
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
AseI ATTAAT 1 cut(s) 317
AspS9I GGNCC 2 cut(s) 391, 441
AsuHPI GGTGA 1 cut(s) 282
AsuII TTCGAA 1 cut(s) 182
AvaII GGWCC 1 cut(s) 391
BanII GRGCYC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 525
BccI CCATC 2 cut(s) 141, 335
BceAI ACGGC 1 cut(s) 81
BcgI CGANNNNNNTGC 2 cut(s) 492, 526
BciT130I CCWGG 2 cut(s) 491, 527
BcoDI GTCTC 3 cut(s) 63, 110, 408
BfaI CTAG 1 cut(s) 330
BglII AGATCT 1 cut(s) 137
BisI GCNGC 2 cut(s) 269, 514
BlsI GCNGC 2 cut(s) 270, 515
Bme1390I CCNGG 2 cut(s) 491, 527
Bme18I GGWCC 1 cut(s) 391
BmeRI GACNNNNNGTC 1 cut(s) 397
BmgT120I GGNCC 2 cut(s) 391, 441
BmrFI CCNGG 2 cut(s) 491, 527
BmsI GCATC 3 cut(s) 160, 316, 525
Bpu10I CCTNAGC 1 cut(s) 110
Bpu14I TTCGAA 1 cut(s) 182
BsaI GGTCTC 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 525
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 5 cut(s) 166, 220, 450, 496, 526
Bse1I ACTGG 1 cut(s) 444
BseBI CCWGG 2 cut(s) 491, 527
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 3 cut(s) 207, 346, 516
BseLI CCNNNNNNNGG 5 cut(s) 166, 220, 450, 496, 526
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 444
BseRI GAGGAG 1 cut(s) 203
BseXI GCAGC 1 cut(s) 525
Bsh1285I CGRYCG 1 cut(s) 78
BshFI GGCC 2 cut(s) 159, 443
BsiEI CGRYCG 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 174
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 5 cut(s) 166, 220, 450, 496, 526
BsmAI GTCTC 3 cut(s) 63, 110, 408
BsmBI CGTCTC 1 cut(s) 408
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 2 cut(s) 159, 443
Bso31I GGTCTC 1 cut(s) 110
Bsp119I TTCGAA 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 174
Bsp1407I TGTACA 1 cut(s) 251
Bsp143I GATC 4 cut(s) 127, 137, 224, 499
BspACI CCGC 5 cut(s) 167, 269, 285, 293, 378
BspANI GGCC 2 cut(s) 159, 443
BspCNI CTCAG 1 cut(s) 50
BspHI TCATGA 1 cut(s) 322
BspPI GGATC 3 cut(s) 122, 232, 507
BspT104I TTCGAA 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 380
BsrGI TGTACA 1 cut(s) 251
BsrI ACTGG 1 cut(s) 444
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 4 cut(s) 127, 137, 224, 499
Bst2UI CCWGG 2 cut(s) 491, 527
Bst4CI ACNGT 2 cut(s) 11, 369
BstAUI TGTACA 1 cut(s) 251
BstBI TTCGAA 1 cut(s) 182
BstC8I GCNNGC 2 cut(s) 43, 462
BstDEI CTNAG 2 cut(s) 37, 110
BstEII GGTNACC 1 cut(s) 288
BstENI CCTNNNNNAGG 1 cut(s) 218
BstF5I GGATG 3 cut(s) 207, 346, 516
BstKTI GATC 4 cut(s) 130, 140, 227, 502
BstMAI GTCTC 3 cut(s) 63, 110, 408
BstMBI GATC 4 cut(s) 127, 137, 224, 499
BstMCI CGRYCG 1 cut(s) 78
BstMWI GCNNNNNNNGC 2 cut(s) 38, 370
BstNI CCWGG 2 cut(s) 491, 527
BstPI GGTNACC 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 489, 525
BstV1I GCAGC 1 cut(s) 525
BstX2I RGATCY 2 cut(s) 137, 224
BstYI RGATCY 2 cut(s) 137, 224
BsuRI GGCC 2 cut(s) 159, 443
BtsCI GGATG 3 cut(s) 207, 346, 516
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 3 cut(s) 7, 365, 471
Cac8I GCNNGC 2 cut(s) 43, 462
CaiI CAGNNNCTG 1 cut(s) 194
CciI TCATGA 1 cut(s) 322
Cfr13I GGNCC 2 cut(s) 391, 441
CseI GACGC 1 cut(s) 103
Csp6I GTAC 1 cut(s) 252
CviAII CATG 4 cut(s) 52, 249, 323, 531
CviJI RGCY 9 cut(s) 41, 159, 172, 241, 263, 308, 432, 443, 460
CviKI_1 RGCY 9 cut(s) 41, 159, 172, 241, 263, 308, 432, 443, 460
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 2 cut(s) 37, 110
DpnI GATC 4 cut(s) 129, 139, 226, 501
DpnII GATC 4 cut(s) 127, 137, 224, 499
DriI GACNNNNNGTC 1 cut(s) 397
EaeI YGGCCR 1 cut(s) 157
Eam1105I GACNNNNNGTC 1 cut(s) 397
Ecl136II GAGCTC 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 391
Eco53kI GAGCTC 1 cut(s) 172
Eco91I GGTNACC 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 172
EcoNI CCTNNNNNAGG 1 cut(s) 218
EcoO65I GGTNACC 1 cut(s) 288
EcoRII CCWGG 2 cut(s) 489, 525
EcoT38I GRGCYC 1 cut(s) 174
Esp3I CGTCTC 1 cut(s) 408
FaeI CATG 4 cut(s) 55, 252, 326, 534
FaiI YATR 5 cut(s) 53, 177, 250, 324, 532
FaqI GGGAC 1 cut(s) 69
FatI CATG 4 cut(s) 51, 248, 322, 530
FauI CCCGC 1 cut(s) 278
Fnu4HI GCNGC 2 cut(s) 269, 514
FokI GGATG 3 cut(s) 194, 353, 503
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 2 cut(s) 269, 514
FspBI CTAG 1 cut(s) 330
GluI GCNGC 2 cut(s) 269, 514
HaeIII GGCC 2 cut(s) 159, 443
HgaI GACGC 1 cut(s) 103
Hin1II CATG 4 cut(s) 55, 252, 326, 534
HinfI GANTC 4 cut(s) 4, 16, 71, 179
HphI GGTGA 1 cut(s) 282
Hpy188I TCNGA 3 cut(s) 137, 146, 196
Hpy188III TCNNGA 4 cut(s) 66, 323, 451, 520
Hpy99I CGWCG 3 cut(s) 50, 403, 507
HpyAV CCTTC 1 cut(s) 417
HpyCH4III ACNGT 2 cut(s) 11, 369
HpyCH4IV ACGT 1 cut(s) 505
HpyCH4V TGCA 1 cut(s) 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 370
HpyF3I CTNAG 2 cut(s) 37, 110
HpySE526I ACGT 1 cut(s) 505
Hsp92II CATG 4 cut(s) 55, 252, 326, 534
Kzo9I GATC 4 cut(s) 127, 137, 224, 499
LmnI GCTCC 2 cut(s) 169, 385
LpnPI CCDG 7 cut(s) 174, 457, 474, 476, 481, 503, 512
Lsp1109I GCAGC 1 cut(s) 525
LweI GCATC 3 cut(s) 160, 316, 525
MaeI CTAG 1 cut(s) 330
MaeII ACGT 1 cut(s) 505
MaeIII GTNAC 2 cut(s) 115, 288
MalI GATC 4 cut(s) 129, 139, 226, 501
MbiI CCGCTC 1 cut(s) 380
MboI GATC 4 cut(s) 127, 137, 224, 499
MflI RGATCY 2 cut(s) 137, 224
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 2 cut(s) 318, 508
MlyI GAGTC 1 cut(s) 65
MnlI CCTC 9 cut(s) 44, 48, 102, 221, 224, 274, 422, 462, 469
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 1 cut(s) 247
MspR9I CCNGG 2 cut(s) 491, 527
MvaI CCWGG 2 cut(s) 491, 527
MwoI GCNNNNNNNGC 2 cut(s) 38, 370
NdeII GATC 4 cut(s) 127, 137, 224, 499
NlaIII CATG 4 cut(s) 55, 252, 326, 534
NmeAIII GCCGAG 2 cut(s) 135, 321
NmuCI GTSAC 2 cut(s) 115, 288
NspV TTCGAA 1 cut(s) 182
PagI TCATGA 1 cut(s) 322
PfeI GAWTC 3 cut(s) 4, 16, 179
PflFI GACNNNGTC 1 cut(s) 74
PkrI GCNGC 2 cut(s) 270, 515
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PshBI ATTAAT 1 cut(s) 317
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 489, 525
PspEI GGTNACC 1 cut(s) 288
PspGI CCWGG 2 cut(s) 489, 525
PspPI GGNCC 2 cut(s) 391, 441
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 2 cut(s) 137, 224
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
RseI CAYNNNNRTG 1 cut(s) 247
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 2 cut(s) 269, 514
Sau3AI GATC 4 cut(s) 127, 137, 224, 499
Sau96I GGNCC 2 cut(s) 391, 441
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 2 cut(s) 491, 527
SduI GDGCHC 1 cut(s) 174
SetI ASST 6 cut(s) 43, 94, 174, 310, 396, 508
SfaNI GCATC 3 cut(s) 160, 316, 525
SfuI TTCGAA 1 cut(s) 182
SinI GGWCC 1 cut(s) 391
SmiMI CAYNNNNRTG 1 cut(s) 247
Sse9I AATT 2 cut(s) 318, 508
SsiI CCGC 5 cut(s) 167, 269, 285, 293, 378
SspMI CTAG 1 cut(s) 330
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 2 cut(s) 489, 525
TaaI ACNGT 2 cut(s) 11, 369
TaiI ACGT 1 cut(s) 508
TaqI TCGA 3 cut(s) 182, 474, 502
TaqII GACCGA 1 cut(s) 64
TasI AATT 2 cut(s) 318, 508
TatI WGTACW 1 cut(s) 251
TauI GCSGC 1 cut(s) 271
TfiI GAWTC 3 cut(s) 4, 16, 179
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 3 cut(s) 14, 372, 471
TseFI GTSAC 2 cut(s) 115, 288
TseI GCWGC 1 cut(s) 513
Tsp45I GTSAC 2 cut(s) 115, 288
TspDTI ATGAA 4 cut(s) 17, 237, 311, 399
TspRI CASTG 3 cut(s) 14, 372, 471
Tth111I GACNNNGTC 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 391
VspI ATTAAT 1 cut(s) 317
XagI CCTNNNNNAGG 1 cut(s) 218
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.