Rw4G029470

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003758
Physical Location & Seq
Forward (+)
56151839 .. 56154037
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029470.1

Sequence Viewer

Length: 516 bp
ATGAATCACTGTGCGATTCAGCAGAACAACGCGTGCGAAGAGATGAGGAATTTCAGAGACGGCGTCGTTTGCCCCAAGCCTCGACGTTTGAGGAACGCAACTCTTCACGAACCCGTCAGATCTTATCGATGGCATCTAGGCCACCAAACTGAGCTCTGTGATGGGAGAGCAGGGTCAGATCTTTTGGAAAACCTCCTTACAAAGGGTTCTTACGGTGTTGAACAATTTTACTGTACAGAAATAGCCTCGTCGCACCCATTTTATAGCGGGTCGCCACCGAGCAGAGTAGCTAACCCACTAATTCAGGATGCTAGATTTGGGGACGAGAAGCTCACCCTATCTTCACCACTGTCACCTATACCTCCCCCTTCAGGTCTGTCATCGTCTCCATCATCCTCTGCAAGGAAAGGAGGCTGTGTTCGGGCCAGTTTTGGGAGCAAGCCAGCAGTGAGGGTTGAGGGGTTTGATTGCCTGGATAGGGATAGGAGAAACTGCAGAATCCCTACCCTGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.83

Weight (kDa)

8.87

Isoelectric Point (pI)

70.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 32
AciI CCGC 1 cut(s) 267
AcsI RAATTY 1 cut(s) 49
AcuI CTGAAG 1 cut(s) 354
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 6 cut(s) 202, 371, 402, 432, 478, 508
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 1 cut(s) 221
AjnI CCWGG 2 cut(s) 471, 507
AloI GAACNNNNNNTCC 2 cut(s) 402, 434
AluBI AGCT 3 cut(s) 154, 290, 331
AluI AGCT 3 cut(s) 154, 290, 331
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 2 cut(s) 51, 390
AoxI GGCC 2 cut(s) 139, 423
ApoI RAATTY 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 423
AsuHPI GGTGA 3 cut(s) 325, 336, 345
BanII GRGCYC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 156
BccI CCATC 3 cut(s) 123, 155, 397
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 2 cut(s) 473, 509
BcoDI GTCTC 2 cut(s) 51, 390
BfaI CTAG 2 cut(s) 137, 312
BfmI CTRYAG 1 cut(s) 493
BglII AGATCT 2 cut(s) 119, 178
Bme1390I CCNGG 2 cut(s) 473, 509
BmgT120I GGNCC 1 cut(s) 423
BmrFI CCNGG 2 cut(s) 473, 509
BmsI GCATC 2 cut(s) 142, 298
Bsa29I ATCGAT 1 cut(s) 127
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 1 cut(s) 507
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 6 cut(s) 202, 371, 402, 432, 478, 508
Bse1I ACTGG 1 cut(s) 426
BseBI CCWGG 2 cut(s) 473, 509
BseCI ATCGAT 1 cut(s) 127
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 2 cut(s) 313, 392
BseLI CCNNNNNNNGG 6 cut(s) 202, 371, 402, 432, 478, 508
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 426
Bsh1236I CGCG 1 cut(s) 32
BshFI GGCC 2 cut(s) 141, 425
BshVI ATCGAT 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 156
BslFI GGGAC 1 cut(s) 335
BslI CCNNNNNNNGG 6 cut(s) 202, 371, 402, 432, 478, 508
BsmAI GTCTC 2 cut(s) 51, 390
BsmBI CGTCTC 2 cut(s) 51, 390
BsmFI GGGAC 1 cut(s) 335
BsnI GGCC 2 cut(s) 141, 425
Bsp1286I GDGCHC 1 cut(s) 156
Bsp1407I TGTACA 1 cut(s) 233
Bsp143I GATC 2 cut(s) 119, 178
BspACI CCGC 1 cut(s) 267
BspANI GGCC 2 cut(s) 141, 425
BspCNI CTCAG 1 cut(s) 142
BspDI ATCGAT 1 cut(s) 127
BspFNI CGCG 1 cut(s) 32
BspMAI CTGCAG 1 cut(s) 497
BsrGI TGTACA 1 cut(s) 233
BsrI ACTGG 1 cut(s) 426
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 2 cut(s) 119, 178
BssNI GRCGYC 1 cut(s) 63
Bst2UI CCWGG 2 cut(s) 473, 509
Bst4CI ACNGT 4 cut(s) 11, 215, 233, 351
Bst6I CTCTTC 2 cut(s) 33, 108
BstACI GRCGYC 1 cut(s) 63
BstAUI TGTACA 1 cut(s) 233
BstC8I GCNNGC 3 cut(s) 34, 440, 444
BstDEI CTNAG 1 cut(s) 150
BstENI CCTNNNNNAGG 2 cut(s) 200, 400
BstF5I GGATG 2 cut(s) 313, 392
BstFNI CGCG 1 cut(s) 32
BstKTI GATC 2 cut(s) 122, 181
BstMAI GTCTC 2 cut(s) 51, 390
BstMBI GATC 2 cut(s) 119, 178
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 2 cut(s) 473, 509
BstSCI CCNGG 2 cut(s) 471, 507
BstSFI CTRYAG 1 cut(s) 493
BstUI CGCG 1 cut(s) 32
BstX2I RGATCY 2 cut(s) 119, 178
BstYI RGATCY 2 cut(s) 119, 178
Bsu15I ATCGAT 1 cut(s) 127
BsuRI GGCC 2 cut(s) 141, 425
BsuTUI ATCGAT 1 cut(s) 127
BtsCI GGATG 2 cut(s) 313, 392
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 3 cut(s) 7, 347, 453
Cac8I GCNNGC 3 cut(s) 34, 440, 444
Cfr13I GGNCC 1 cut(s) 423
ClaI ATCGAT 1 cut(s) 127
CseI GACGC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 234
CviJI RGCY 9 cut(s) 79, 141, 154, 245, 290, 331, 414, 425, 442
CviKI_1 RGCY 9 cut(s) 79, 141, 154, 245, 290, 331, 414, 425, 442
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 1 cut(s) 150
DpnI GATC 2 cut(s) 121, 180
DpnII GATC 2 cut(s) 119, 178
Eam1104I CTCTTC 2 cut(s) 33, 108
EarI CTCTTC 2 cut(s) 33, 108
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 354
EcoICRI GAGCTC 1 cut(s) 154
EcoNI CCTNNNNNAGG 2 cut(s) 200, 400
EcoRII CCWGG 2 cut(s) 471, 507
EcoT38I GRGCYC 1 cut(s) 156
Esp3I CGTCTC 2 cut(s) 51, 390
FaiI YATR 3 cut(s) 264, 359, 514
FaqI GGGAC 1 cut(s) 335
FauI CCCGC 1 cut(s) 260
FokI GGATG 2 cut(s) 320, 379
FriOI GRGCYC 1 cut(s) 156
FspBI CTAG 2 cut(s) 137, 312
HaeIII GGCC 2 cut(s) 141, 425
HgaI GACGC 1 cut(s) 52
Hin1I GRCGYC 1 cut(s) 63
HinfI GANTC 3 cut(s) 4, 16, 498
HphI GGTGA 3 cut(s) 325, 336, 345
Hpy188I TCNGA 3 cut(s) 56, 119, 178
Hpy188III TCNNGA 2 cut(s) 107, 305
Hpy99I CGWCG 3 cut(s) 68, 87, 253
HpyAV CCTTC 1 cut(s) 378
HpyCH4III ACNGT 4 cut(s) 11, 215, 233, 351
HpyCH4IV ACGT 1 cut(s) 85
HpyCH4V TGCA 2 cut(s) 401, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 1 cut(s) 150
HpySE526I ACGT 1 cut(s) 85
Hsp92I GRCGYC 1 cut(s) 63
Kzo9I GATC 2 cut(s) 119, 178
LmnI GCTCC 1 cut(s) 435
LpnPI CCDG 8 cut(s) 156, 290, 357, 439, 456, 458, 485, 494
LweI GCATC 2 cut(s) 142, 298
MaeI CTAG 2 cut(s) 137, 312
MaeII ACGT 1 cut(s) 85
MaeIII GTNAC 1 cut(s) 351
MalI GATC 2 cut(s) 121, 180
MboI GATC 2 cut(s) 119, 178
MboII GAAGA 3 cut(s) 50, 95, 333
MflI RGATCY 2 cut(s) 119, 178
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 3 cut(s) 49, 224, 300
MluI ACGCGT 1 cut(s) 30
MspR9I CCNGG 2 cut(s) 473, 509
MvaI CCWGG 2 cut(s) 473, 509
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 1 cut(s) 69
NdeII GATC 2 cut(s) 119, 178
NmuCI GTSAC 1 cut(s) 351
PfeI GAWTC 3 cut(s) 4, 16, 498
PflFI GACNNNGTC 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 471, 507
PspGI CCWGG 2 cut(s) 471, 507
PspPI GGNCC 1 cut(s) 423
PstI CTGCAG 1 cut(s) 497
PsuI RGATCY 2 cut(s) 119, 178
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SacI GAGCTC 1 cut(s) 156
Sau3AI GATC 2 cut(s) 119, 178
Sau96I GGNCC 1 cut(s) 423
ScrFI CCNGG 2 cut(s) 473, 509
SduI GDGCHC 1 cut(s) 156
SetI ASST 8 cut(s) 88, 156, 195, 292, 333, 358, 364, 376
SfaNI GCATC 2 cut(s) 142, 298
SfcI CTRYAG 1 cut(s) 493
Sse9I AATT 3 cut(s) 49, 224, 300
SsiI CCGC 1 cut(s) 267
SspMI CTAG 2 cut(s) 137, 312
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 471, 507
TaaI ACNGT 4 cut(s) 11, 215, 233, 351
TaiI ACGT 1 cut(s) 88
TaqI TCGA 2 cut(s) 82, 127
TasI AATT 3 cut(s) 49, 224, 300
TatI WGTACW 1 cut(s) 233
TfiI GAWTC 3 cut(s) 4, 16, 498
TscAI CASTG 3 cut(s) 14, 354, 453
TseFI GTSAC 1 cut(s) 351
Tsp45I GTSAC 1 cut(s) 351
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 14, 354, 453
Tth111I GACNNNGTC 1 cut(s) 62
XagI CCTNNNNNAGG 2 cut(s) 200, 400
XapI RAATTY 1 cut(s) 49
XspI CTAG 2 cut(s) 137, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.