MD16G1042500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003758
Physical Location & Seq
Forward (+)
3006224 .. 3008236
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1042500.v1.1.491

Sequence Viewer

Length: 522 bp
ATGAATCACTGTGCGATGCAGCAGGGCAGTGCTCTCTCAGCTTGCGAAGACATGAGGTCTTGCTCCAGAGACTCCGTCGTCTGCCCCAAGCCCCGCCGCCTCGGCCTCCTCAACGATCCCGTCAGATCTTTCCGGTGGCATTTCGGCCACCAGGCGGAGTTCTGTGATTCAAATGCTGGGTCTGACATTTTAGACAATATTCTCACTAAGGGATCTTTTGGTGCTGAACAATTTTCGTGTACAGAAATGGCCTCGCAGCACCCATTTTATAGCGGGTCGCCGCCTAGCAGAGTAGCTAACCCATTAATTCAGGACGCTCGATTTGGGGATGAGCAGCTCATCTCGTCCTCAGCACTGTCGCCAATACCTGCCCTGTCAGGTCAGTCATCGTCTCCTTCCTCGTCCGCGAGGAAAGGAGGCTGTGTTCGGACCAATTTTGGGAGCAAACCGGCAGTGAGGGTTGAGGGGTTTGATTGCCGGGACAGGGATCAGAGGAACCGCAGCATCCCTACCCTGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

18.71

Weight (kDa)

8.16

Isoelectric Point (pI)

74.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 376
AccII CGCG 1 cut(s) 407
AciI CCGC 7 cut(s) 94, 97, 155, 273, 281, 405, 499
AclWI GGATC 3 cut(s) 110, 220, 495
AcoI YGGCCR 1 cut(s) 145
AfaI GTAC 1 cut(s) 241
AfiI CCNNNNNNNGG 4 cut(s) 154, 438, 484, 514
AgsI TTSAA 1 cut(s) 171
AhdI GACNNNNNGTC 1 cut(s) 55
AjnI CCWGG 2 cut(s) 150, 513
AloI GAACNNNNNNTCC 2 cut(s) 408, 440
AluBI AGCT 3 cut(s) 41, 296, 337
AluI AGCT 3 cut(s) 41, 296, 337
Alw21I GWGCWC 1 cut(s) 34
Alw26I GTCTC 2 cut(s) 63, 396
AlwI GGATC 3 cut(s) 110, 220, 495
AoxI GGCC 3 cut(s) 103, 145, 249
ApeKI GCWGC 4 cut(s) 19, 256, 334, 501
ArsI GACNNNNNNTTYG 4 cut(s) 164, 196, 305, 337
AseI ATTAAT 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 429
AsuC2I CCSGG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 34
BbvCI CCTCAGC 1 cut(s) 349
BbvI GCAGC 4 cut(s) 31, 268, 346, 513
BciT130I CCWGG 2 cut(s) 152, 515
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 2 cut(s) 63, 396
BfaI CTAG 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 376
BglI GCCNNNNNGGC 1 cut(s) 102
BglII AGATCT 1 cut(s) 125
BisI GCNGC 6 cut(s) 20, 97, 257, 281, 335, 502
BlsI GCNGC 6 cut(s) 21, 98, 258, 282, 336, 503
Bme1390I CCNGG 3 cut(s) 152, 479, 515
Bme18I GGWCC 1 cut(s) 429
BmeRI GACNNNNNGTC 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 429
BmiI GGNNCC 1 cut(s) 497
BmrFI CCNGG 3 cut(s) 152, 479, 515
BmsI GCATC 2 cut(s) 6, 513
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 49
Bpu10I CCTNAGC 1 cut(s) 349
BpuMI CCSGG 1 cut(s) 479
BsaJI CCNNGG 2 cut(s) 100, 513
BsaWI WCCGGW 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 438, 484, 514
Bse118I RCCGGY 1 cut(s) 448
BseBI CCWGG 2 cut(s) 152, 515
BseDI CCNNGG 2 cut(s) 100, 513
BseGI GGATG 2 cut(s) 334, 504
BseLI CCNNNNNNNGG 4 cut(s) 154, 438, 484, 514
BseMII CTCAG 2 cut(s) 51, 363
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 4 cut(s) 31, 268, 346, 513
BseYI CCCAGC 1 cut(s) 176
Bsh1236I CGCG 1 cut(s) 407
BshFI GGCC 3 cut(s) 105, 147, 251
BsiHKAI GWGCWC 1 cut(s) 34
BsiSI CCGG 3 cut(s) 133, 449, 478
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 4 cut(s) 154, 438, 484, 514
BsmAI GTCTC 2 cut(s) 63, 396
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 1 cut(s) 494
BsnI GGCC 3 cut(s) 105, 147, 251
Bsp1286I GDGCHC 1 cut(s) 34
Bsp1407I TGTACA 1 cut(s) 239
Bsp143I GATC 4 cut(s) 115, 125, 212, 487
BspACI CCGC 7 cut(s) 94, 97, 155, 273, 281, 405, 499
BspANI GGCC 3 cut(s) 105, 147, 251
BspCNI CTCAG 2 cut(s) 50, 362
BspFNI CGCG 1 cut(s) 407
BspLI GGNNCC 1 cut(s) 497
BspMI ACCTGC 1 cut(s) 376
BspPI GGATC 3 cut(s) 110, 220, 495
BsrFI RCCGGY 1 cut(s) 448
BsrGI TGTACA 1 cut(s) 239
BssAI RCCGGY 1 cut(s) 448
BssECI CCNNGG 2 cut(s) 100, 513
BssMI GATC 4 cut(s) 115, 125, 212, 487
Bst2UI CCWGG 2 cut(s) 152, 515
Bst4CI ACNGT 2 cut(s) 11, 357
BstAUI TGTACA 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 3 cut(s) 37, 207, 349
BstF5I GGATG 2 cut(s) 334, 504
BstFNI CGCG 1 cut(s) 407
BstKTI GATC 4 cut(s) 118, 128, 215, 490
BstMAI GTCTC 2 cut(s) 63, 396
BstMBI GATC 4 cut(s) 115, 125, 212, 487
BstMWI GCNNNNNNNGC 2 cut(s) 38, 102
BstNI CCWGG 2 cut(s) 152, 515
BstSCI CCNGG 3 cut(s) 150, 477, 513
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 4 cut(s) 31, 268, 346, 513
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 2 cut(s) 125, 212
BstYI RGATCY 2 cut(s) 125, 212
BsuRI GGCC 3 cut(s) 105, 147, 251
BtgZI GCGATG 1 cut(s) 29
BtsCI GGATG 2 cut(s) 334, 504
BtsI GCAGTG 2 cut(s) 34, 459
BtsIMutI CAGTG 4 cut(s) 7, 34, 353, 459
BveI ACCTGC 1 cut(s) 376
Cac8I GCNNGC 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 448
Cfr13I GGNCC 1 cut(s) 429
CseI GACGC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 240
CviAII CATG 1 cut(s) 52
CviJI RGCY 8 cut(s) 41, 91, 105, 147, 251, 296, 337, 420
CviKI_1 RGCY 8 cut(s) 41, 91, 105, 147, 251, 296, 337, 420
CviQI GTAC 1 cut(s) 240
DdeI CTNAG 3 cut(s) 37, 207, 349
DpnI GATC 4 cut(s) 117, 127, 214, 489
DpnII GATC 4 cut(s) 115, 125, 212, 487
DrdI GACNNNNNNGTC 1 cut(s) 77
DriI GACNNNNNGTC 1 cut(s) 55
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 145
Eam1105I GACNNNNNGTC 1 cut(s) 55
EciI GGCGGA 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 429
EcoRII CCWGG 2 cut(s) 150, 513
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 1 cut(s) 55
FaiI YATR 3 cut(s) 53, 270, 520
FaqI GGGAC 1 cut(s) 494
FatI CATG 1 cut(s) 51
FauI CCCGC 2 cut(s) 101, 266
Fnu4HI GCNGC 6 cut(s) 20, 97, 257, 281, 335, 502
FokI GGATG 2 cut(s) 341, 491
Fsp4HI GCNGC 6 cut(s) 20, 97, 257, 281, 335, 502
FspBI CTAG 1 cut(s) 285
GluI GCNGC 6 cut(s) 20, 97, 257, 281, 335, 502
GsaI CCCAGC 1 cut(s) 180
GsuI CTGGAG 1 cut(s) 49
HaeIII GGCC 3 cut(s) 105, 147, 251
HapII CCGG 3 cut(s) 133, 449, 478
HgaI GACGC 1 cut(s) 323
Hin1II CATG 1 cut(s) 55
HinfI GANTC 3 cut(s) 4, 71, 167
HpaII CCGG 3 cut(s) 133, 449, 478
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 4 cut(s) 125, 184, 429, 492
Hpy188III TCNNGA 2 cut(s) 66, 311
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 405
HpyCH4III ACNGT 2 cut(s) 11, 357
HpyCH4V TGCA 1 cut(s) 19
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 102
HpyF3I CTNAG 3 cut(s) 37, 207, 349
Hsp92II CATG 1 cut(s) 55
Kzo9I GATC 4 cut(s) 115, 125, 212, 487
LmnI GCTCC 2 cut(s) 68, 441
Lsp1109I GCAGC 4 cut(s) 31, 268, 346, 513
LweI GCATC 2 cut(s) 6, 513
MaeI CTAG 1 cut(s) 285
MalI GATC 4 cut(s) 117, 127, 214, 489
MboI GATC 4 cut(s) 115, 125, 212, 487
MboII GAAGA 1 cut(s) 59
MflI RGATCY 2 cut(s) 125, 212
MhlI GDGCHC 1 cut(s) 34
MluCI AATT 3 cut(s) 230, 306, 433
MlyI GAGTC 1 cut(s) 65
MseI TTAA 1 cut(s) 305
MspI CCGG 3 cut(s) 133, 449, 478
MspR9I CCNGG 3 cut(s) 152, 479, 515
MvaI CCWGG 2 cut(s) 152, 515
MvnI CGCG 1 cut(s) 407
MwoI GCNNNNNNNGC 2 cut(s) 38, 102
NciI CCSGG 1 cut(s) 479
NdeII GATC 4 cut(s) 115, 125, 212, 487
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 1 cut(s) 497
NmeAIII GCCGAG 1 cut(s) 81
PfeI GAWTC 2 cut(s) 4, 167
PflFI GACNNNGTC 1 cut(s) 74
PkrI GCNGC 6 cut(s) 21, 98, 258, 282, 336, 503
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PshBI ATTAAT 1 cut(s) 305
Psp6I CCWGG 2 cut(s) 150, 513
PspFI CCCAGC 1 cut(s) 176
PspGI CCWGG 2 cut(s) 150, 513
PspN4I GGNNCC 1 cut(s) 497
PspPI GGNCC 1 cut(s) 429
PsuI RGATCY 2 cut(s) 125, 212
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 1 cut(s) 241
RsaNI GTAC 1 cut(s) 240
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 6 cut(s) 20, 97, 257, 281, 335, 502
Sau3AI GATC 4 cut(s) 115, 125, 212, 487
Sau96I GGNCC 1 cut(s) 429
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 3 cut(s) 152, 479, 515
SduI GDGCHC 1 cut(s) 34
SetI ASST 6 cut(s) 43, 59, 298, 339, 370, 382
SfaNI GCATC 2 cut(s) 6, 513
SinI GGWCC 1 cut(s) 429
Sse9I AATT 3 cut(s) 230, 306, 433
SsiI CCGC 7 cut(s) 94, 97, 155, 273, 281, 405, 499
SspI AATATT 1 cut(s) 199
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 3 cut(s) 150, 477, 513
TaaI ACNGT 2 cut(s) 11, 357
TaqI TCGA 1 cut(s) 319
TasI AATT 3 cut(s) 230, 306, 433
TatI WGTACW 1 cut(s) 239
TauI GCSGC 2 cut(s) 99, 283
TfiI GAWTC 2 cut(s) 4, 167
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 4 cut(s) 14, 34, 360, 459
TseI GCWGC 4 cut(s) 19, 256, 334, 501
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 4 cut(s) 14, 34, 360, 459
Tth111I GACNNNGTC 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 429
VspI ATTAAT 1 cut(s) 305
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.