Rw4G029480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003758
Physical Location & Seq
Forward (+)
56155628 .. 56156397
770 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029480.1

Sequence Viewer

Length: 465 bp
ATGAACCACTGCGTGATACAGCAGAGCAGCGCCCTTTCGGTTTGCGAAGAGATGAGGACGGCGAGAGACTCCGTCGTTTGCCCCAAACCGCGCCGTTTCGGACTCCGCAACCCATCAGGGGAGCTCTGTGATTCAAAGGCAGGCTCTGATGCTTTGGACTGTATCTTAACAAAGGGGGTTGAACAGTTTTCTTGTACAGAAATAGGCTCGCCGCACCCATTTTTCAGCGGATCGCCGCCGAGCAGAGTAGCTAATCCATTGATTCGGGATGCTAGATTTGGCAGTGAGAAGCTCACTCCAGTGTCGCCGCTTTCACCGGTGTCGTCATCGTCTCCATCATCTTCCACAAGGAATGGAGGAGGCTGTGTTCGTGCTATTTTCGGGAGTAAACCAACAGTGAGGGTCGAGGGGTTTGATTGCCTGGACAGGGATAGCCGAAATTGCGGCATCCCTTCCCTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

16.32

Weight (kDa)

8.43

Isoelectric Point (pI)

70.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 91
AciI CCGC 7 cut(s) 89, 106, 212, 228, 236, 308, 444
AclWI GGATC 1 cut(s) 238
AdeI CACNNNGTG 1 cut(s) 13
AfaI GTAC 1 cut(s) 196
AfiI CCNNNNNNNGG 3 cut(s) 118, 427, 457
AgeI ACCGGT 1 cut(s) 316
AgsI TTSAA 2 cut(s) 135, 182
AjnI CCWGG 2 cut(s) 420, 456
AleI CACNNNNGTG 1 cut(s) 299
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
AluBI AGCT 3 cut(s) 124, 251, 292
AluI AGCT 3 cut(s) 124, 251, 292
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 60, 336
AlwI GGATC 1 cut(s) 238
AlwNI CAGNNNCTG 1 cut(s) 146
ApeKI GCWGC 1 cut(s) 27
AsiGI ACCGGT 1 cut(s) 316
AspLEI GCGC 2 cut(s) 32, 93
AsuHPI GGTGA 1 cut(s) 306
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 39
BccI CCATC 2 cut(s) 121, 343
BceAI ACGGC 2 cut(s) 75, 78
BciT130I CCWGG 2 cut(s) 422, 458
BcoDI GTCTC 2 cut(s) 60, 336
BfaI CTAG 1 cut(s) 273
BfoI RGCGCY 1 cut(s) 33
BisI GCNGC 5 cut(s) 28, 212, 236, 308, 445
BlsI GCNGC 5 cut(s) 29, 213, 237, 309, 446
Bme1390I CCNGG 2 cut(s) 422, 458
BmrFI CCNGG 2 cut(s) 422, 458
BmsI GCATC 3 cut(s) 139, 259, 456
BpmI CTGGAG 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 456
BsaWI WCCGGW 1 cut(s) 316
BsaXI ACNNNNNCTCC 2 cut(s) 351, 381
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 427, 457
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 299
BseBI CCWGG 2 cut(s) 422, 458
BseDI CCNNGG 1 cut(s) 456
BseGI GGATG 2 cut(s) 274, 447
BseLI CCNNNNNNNGG 3 cut(s) 118, 427, 457
BseNI ACTGG 1 cut(s) 299
BseRI GAGGAG 1 cut(s) 372
BseXI GCAGC 1 cut(s) 39
Bsh1236I CGCG 1 cut(s) 91
BshTI ACCGGT 1 cut(s) 316
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 1 cut(s) 317
BslI CCNNNNNNNGG 3 cut(s) 118, 427, 457
BsmAI GTCTC 2 cut(s) 60, 336
BsmBI CGTCTC 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 126
Bsp1407I TGTACA 1 cut(s) 194
Bsp143I GATC 1 cut(s) 230
BspACI CCGC 7 cut(s) 89, 106, 212, 228, 236, 308, 444
BspFNI CGCG 1 cut(s) 91
BspPI GGATC 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 316
BsrGI TGTACA 1 cut(s) 194
BsrI ACTGG 1 cut(s) 299
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 1 cut(s) 456
BssMI GATC 1 cut(s) 230
Bst2UI CCWGG 2 cut(s) 422, 458
Bst4CI ACNGT 3 cut(s) 161, 186, 397
Bst6I CTCTTC 1 cut(s) 42
BstAUI TGTACA 1 cut(s) 194
BstC8I GCNNGC 2 cut(s) 142, 209
BstF5I GGATG 2 cut(s) 274, 447
BstFNI CGCG 1 cut(s) 91
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 2 cut(s) 32, 93
BstKTI GATC 1 cut(s) 233
BstMAI GTCTC 2 cut(s) 60, 336
BstMBI GATC 1 cut(s) 230
BstMWI GCNNNNNNNGC 1 cut(s) 441
BstNI CCWGG 2 cut(s) 422, 458
BstSCI CCNGG 2 cut(s) 420, 456
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 39
BtsCI GGATG 2 cut(s) 274, 447
BtsI GCAGTG 2 cut(s) 7, 289
BtsIMutI CAGTG 4 cut(s) 7, 289, 306, 402
Cac8I GCNNGC 2 cut(s) 142, 209
CaiI CAGNNNCTG 1 cut(s) 146
CfoI GCGC 2 cut(s) 32, 93
Cfr10I RCCGGY 1 cut(s) 316
Csp6I GTAC 1 cut(s) 195
CspAI ACCGGT 1 cut(s) 316
CviJI RGCY 8 cut(s) 124, 144, 207, 251, 292, 363, 435, 461
CviKI_1 RGCY 8 cut(s) 124, 144, 207, 251, 292, 363, 435, 461
CviQI GTAC 1 cut(s) 195
DpnI GATC 1 cut(s) 232
DpnII GATC 1 cut(s) 230
DraIII CACNNNGTG 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 124
EcoRII CCWGG 2 cut(s) 420, 456
EcoT38I GRGCYC 1 cut(s) 126
Esp3I CGTCTC 1 cut(s) 336
Fnu4HI GCNGC 5 cut(s) 28, 212, 236, 308, 445
FokI GGATG 2 cut(s) 281, 434
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 5 cut(s) 28, 212, 236, 308, 445
FspBI CTAG 1 cut(s) 273
GlaI GCGC 2 cut(s) 31, 92
GluI GCNGC 5 cut(s) 28, 212, 236, 308, 445
GsuI CTGGAG 1 cut(s) 282
HaeII RGCGCY 1 cut(s) 33
HapII CCGG 1 cut(s) 317
HhaI GCGC 2 cut(s) 32, 93
Hin6I GCGC 2 cut(s) 30, 91
HinP1I GCGC 2 cut(s) 30, 91
HinfI GANTC 4 cut(s) 68, 102, 131, 262
HpaII CCGG 1 cut(s) 317
HphI GGTGA 1 cut(s) 306
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 2 cut(s) 101, 148
Hpy188III TCNNGA 2 cut(s) 266, 382
Hpy8I GTNNAC 1 cut(s) 389
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 462
HpyCH4III ACNGT 3 cut(s) 161, 186, 397
HpyF10VI GCNNNNNNNGC 1 cut(s) 441
HspAI GCGC 2 cut(s) 30, 91
Kzo9I GATC 1 cut(s) 230
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 8 cut(s) 102, 126, 312, 330, 407, 412, 434, 443
Lsp1109I GCAGC 1 cut(s) 39
LweI GCATC 3 cut(s) 139, 259, 456
MaeI CTAG 1 cut(s) 273
MalI GATC 1 cut(s) 232
MboI GATC 1 cut(s) 230
MboII GAAGA 2 cut(s) 59, 333
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 1 cut(s) 439
MlyI GAGTC 2 cut(s) 62, 96
MnlI CCTC 5 cut(s) 48, 350, 353, 393, 400
MseI TTAA 1 cut(s) 167
MslI CAYNNNNRTG 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 1 cut(s) 317
MspR9I CCNGG 2 cut(s) 422, 458
MvaI CCWGG 2 cut(s) 422, 458
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 1 cut(s) 441
NdeII GATC 1 cut(s) 230
NmeAIII GCCGAG 1 cut(s) 264
OliI CACNNNNGTG 1 cut(s) 299
PfeI GAWTC 2 cut(s) 131, 262
PflFI GACNNNGTC 1 cut(s) 71
PinAI ACCGGT 1 cut(s) 316
PkrI GCNGC 5 cut(s) 29, 213, 237, 309, 446
PleI GAGTC 2 cut(s) 62, 96
PpsI GAGTC 2 cut(s) 62, 96
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 420, 456
PspGI CCWGG 2 cut(s) 420, 456
PstNI CAGNNNCTG 1 cut(s) 146
PsyI GACNNNGTC 1 cut(s) 71
RsaI GTAC 1 cut(s) 196
RsaNI GTAC 1 cut(s) 195
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 5 cut(s) 28, 212, 236, 308, 445
Sau3AI GATC 1 cut(s) 230
SchI GAGTC 2 cut(s) 62, 96
ScrFI CCNGG 2 cut(s) 422, 458
SduI GDGCHC 1 cut(s) 126
SetI ASST 3 cut(s) 126, 253, 294
SfaNI GCATC 3 cut(s) 139, 259, 456
SgrAI CRCCGGYG 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 299
Sse9I AATT 1 cut(s) 439
SsiI CCGC 7 cut(s) 89, 106, 212, 228, 236, 308, 444
SspMI CTAG 1 cut(s) 273
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 420, 456
TaaI ACNGT 3 cut(s) 161, 186, 397
TaqI TCGA 1 cut(s) 405
TasI AATT 1 cut(s) 439
TatI WGTACW 1 cut(s) 194
TauI GCSGC 4 cut(s) 214, 238, 310, 447
TfiI GAWTC 2 cut(s) 131, 262
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TscAI CASTG 4 cut(s) 14, 289, 306, 402
TseI GCWGC 1 cut(s) 27
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 4 cut(s) 14, 289, 306, 402
Tth111I GACNNNGTC 1 cut(s) 71
XspI CTAG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.