MD13G1041600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003758
Physical Location & Seq
Forward (+)
2852646 .. 2854481
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1041600.v1.1.491

Sequence Viewer

Length: 522 bp
ATGAATCACTGTGGGATACAGCAAGGCAATGCGCTCTCAGCTTGCGAAGACATGAGGTCTTGCTCCAGGGACTCCGTCGTCTGCCCCAAACCTCGCCGCCTCGGCCTCCTCAACGATCCCGTCAGATCTTTCCGATGGCATTTCGGCCACCAGGCGGAGTTCTGTGATTCAAAAGCCGGGTCTGATATTTTAGACAATATTCTCACTAAGGGATCCTTTGGTGTTGAACAATTTTCGTGTACAGAAATGGCCTCGCAGCACCCATTTTATTGCGGATCGCCACCGAGCAGAGTAGCTAACCCATTAATTCAGGACGCTCGATTTGGGGATGAGGAGCTCCTCTCGTCCCCATCACTGTCGCCAATACCTGCCCCGTCAGGTCAATCATCGTCTCCTTCCTCGTCCACGAGGAAAGGAGGCTGTGCTAGGACCAATTTTGGGAGCAAACCGGCAGTGAGGGTCGAGGGGTTTGATTGCCTGGACAGGGATCGAAGGAACTGCAGCATCCCTACCCTGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

18.71

Weight (kDa)

7.53

Isoelectric Point (pI)

81.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 376
AciI CCGC 3 cut(s) 97, 155, 273
AclWI GGATC 5 cut(s) 110, 207, 220, 283, 495
AcoI YGGCCR 1 cut(s) 145
AfaI GTAC 1 cut(s) 241
AfiI CCNNNNNNNGG 4 cut(s) 154, 438, 484, 514
AgsI TTSAA 2 cut(s) 171, 227
AhdI GACNNNNNGTC 1 cut(s) 55
AjnI CCWGG 4 cut(s) 65, 150, 477, 513
AluBI AGCT 3 cut(s) 41, 296, 337
AluI AGCT 3 cut(s) 41, 296, 337
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 396
AlwI GGATC 5 cut(s) 110, 207, 220, 283, 495
AoxI GGCC 3 cut(s) 103, 145, 249
ApeKI GCWGC 2 cut(s) 256, 501
ArsI GACNNNNNNTTYG 4 cut(s) 164, 196, 305, 337
AseI ATTAAT 1 cut(s) 305
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 429
AsuC2I CCSGG 1 cut(s) 178
AvaII GGWCC 1 cut(s) 429
BamHI GGATCC 1 cut(s) 212
BanII GRGCYC 1 cut(s) 339
BauI CACGAG 1 cut(s) 406
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 2 cut(s) 268, 513
BccI CCATC 2 cut(s) 129, 358
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BciT130I CCWGG 4 cut(s) 67, 152, 479, 515
BciVI GTATCC 1 cut(s) 9
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 396
BfaI CTAG 1 cut(s) 426
BfmI CTRYAG 1 cut(s) 499
BfuAI ACCTGC 1 cut(s) 376
BfuI GTATCC 1 cut(s) 9
BglI GCCNNNNNGGC 1 cut(s) 102
BglII AGATCT 1 cut(s) 125
BisI GCNGC 3 cut(s) 97, 257, 502
BlsI GCNGC 3 cut(s) 98, 258, 503
Bme1390I CCNGG 5 cut(s) 67, 152, 178, 479, 515
Bme18I GGWCC 1 cut(s) 429
BmeRI GACNNNNNGTC 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 429
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 5 cut(s) 67, 152, 178, 479, 515
BmsI GCATC 1 cut(s) 513
BpiI GAAGAC 1 cut(s) 54
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 1 cut(s) 49
BpuMI CCSGG 1 cut(s) 178
BsaJI CCNNGG 3 cut(s) 66, 100, 513
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 438, 484, 514
Bse118I RCCGGY 1 cut(s) 448
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 4 cut(s) 67, 152, 479, 515
BseDI CCNNGG 3 cut(s) 66, 100, 513
BseGI GGATG 2 cut(s) 334, 504
BseLI CCNNNNNNNGG 4 cut(s) 154, 438, 484, 514
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 51
BseRI GAGGAG 3 cut(s) 98, 329, 347
BseXI GCAGC 2 cut(s) 268, 513
BshFI GGCC 3 cut(s) 105, 147, 251
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 2 cut(s) 177, 449
BslFI GGGAC 2 cut(s) 83, 331
BslI CCNNNNNNNGG 4 cut(s) 154, 438, 484, 514
BsmAI GTCTC 1 cut(s) 396
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 2 cut(s) 83, 331
BsnI GGCC 3 cut(s) 105, 147, 251
Bsp1286I GDGCHC 1 cut(s) 339
Bsp1407I TGTACA 1 cut(s) 239
Bsp143I GATC 5 cut(s) 115, 125, 212, 275, 487
BspACI CCGC 3 cut(s) 97, 155, 273
BspANI GGCC 3 cut(s) 105, 147, 251
BspCNI CTCAG 1 cut(s) 50
BspLI GGNNCC 1 cut(s) 214
BspMAI CTGCAG 1 cut(s) 503
BspMI ACCTGC 1 cut(s) 376
BspPI GGATC 5 cut(s) 110, 207, 220, 283, 495
BsrDI GCAATG 1 cut(s) 34
BsrFI RCCGGY 1 cut(s) 448
BsrGI TGTACA 1 cut(s) 239
BssAI RCCGGY 1 cut(s) 448
BssECI CCNNGG 3 cut(s) 66, 100, 513
BssMI GATC 5 cut(s) 115, 125, 212, 275, 487
BssSI CACGAG 1 cut(s) 406
Bst2BI CACGAG 1 cut(s) 406
Bst2UI CCWGG 4 cut(s) 67, 152, 479, 515
Bst4CI ACNGT 2 cut(s) 11, 357
BstAUI TGTACA 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 2 cut(s) 37, 207
BstF5I GGATG 2 cut(s) 334, 504
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 5 cut(s) 118, 128, 215, 278, 490
BstMAI GTCTC 1 cut(s) 396
BstMBI GATC 5 cut(s) 115, 125, 212, 275, 487
BstMWI GCNNNNNNNGC 2 cut(s) 38, 102
BstNI CCWGG 4 cut(s) 67, 152, 479, 515
BstSCI CCNGG 5 cut(s) 65, 150, 176, 477, 513
BstSFI CTRYAG 1 cut(s) 499
BstV1I GCAGC 2 cut(s) 268, 513
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 2 cut(s) 125, 212
BstYI RGATCY 2 cut(s) 125, 212
BsuI GTATCC 1 cut(s) 9
BsuRI GGCC 3 cut(s) 105, 147, 251
BtsCI GGATG 2 cut(s) 334, 504
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 3 cut(s) 7, 353, 459
BveI ACCTGC 1 cut(s) 376
Cac8I GCNNGC 1 cut(s) 43
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 448
Cfr13I GGNCC 1 cut(s) 429
CseI GACGC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 240
CviAII CATG 1 cut(s) 52
CviJI RGCY 8 cut(s) 41, 105, 147, 176, 251, 296, 337, 420
CviKI_1 RGCY 8 cut(s) 41, 105, 147, 176, 251, 296, 337, 420
CviQI GTAC 1 cut(s) 240
DdeI CTNAG 2 cut(s) 37, 207
DpnI GATC 5 cut(s) 117, 127, 214, 277, 489
DpnII GATC 5 cut(s) 115, 125, 212, 275, 487
DrdI GACNNNNNNGTC 1 cut(s) 77
DriI GACNNNNNGTC 1 cut(s) 55
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 145
Eam1105I GACNNNNNGTC 1 cut(s) 55
EciI GGCGGA 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 429
Eco53kI GAGCTC 1 cut(s) 337
EcoICRI GAGCTC 1 cut(s) 337
EcoRII CCWGG 4 cut(s) 65, 150, 477, 513
EcoT38I GRGCYC 1 cut(s) 339
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 1 cut(s) 55
FaiI YATR 2 cut(s) 53, 520
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 2 cut(s) 83, 331
FatI CATG 1 cut(s) 51
Fnu4HI GCNGC 3 cut(s) 97, 257, 502
FokI GGATG 2 cut(s) 341, 491
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 3 cut(s) 97, 257, 502
FspBI CTAG 1 cut(s) 426
GlaI GCGC 1 cut(s) 33
GluI GCNGC 3 cut(s) 97, 257, 502
GsuI CTGGAG 1 cut(s) 49
HaeIII GGCC 3 cut(s) 105, 147, 251
HapII CCGG 2 cut(s) 177, 449
HgaI GACGC 1 cut(s) 323
HhaI GCGC 1 cut(s) 34
Hin1II CATG 1 cut(s) 55
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HinfI GANTC 3 cut(s) 4, 71, 167
HpaII CCGG 2 cut(s) 177, 449
Hpy166II GTNNAC 2 cut(s) 240, 405
Hpy188I TCNGA 3 cut(s) 125, 134, 184
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 2 cut(s) 240, 405
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 405, 486
HpyCH4III ACNGT 2 cut(s) 11, 357
HpyCH4V TGCA 1 cut(s) 501
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 102
HpyF3I CTNAG 2 cut(s) 37, 207
Hsp92II CATG 1 cut(s) 55
HspAI GCGC 1 cut(s) 32
Kzo9I GATC 5 cut(s) 115, 125, 212, 275, 487
LmnI GCTCC 4 cut(s) 68, 334, 342, 441
Lsp1109I GCAGC 2 cut(s) 268, 513
LweI GCATC 1 cut(s) 513
MaeI CTAG 1 cut(s) 426
MalI GATC 5 cut(s) 117, 127, 214, 277, 489
MboI GATC 5 cut(s) 115, 125, 212, 275, 487
MboII GAAGA 1 cut(s) 59
MflI RGATCY 2 cut(s) 125, 212
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 3 cut(s) 230, 306, 433
MlyI GAGTC 1 cut(s) 65
MseI TTAA 1 cut(s) 305
MspI CCGG 2 cut(s) 177, 449
MspR9I CCNGG 5 cut(s) 67, 152, 178, 479, 515
MvaI CCWGG 4 cut(s) 67, 152, 479, 515
MwoI GCNNNNNNNGC 2 cut(s) 38, 102
NciI CCSGG 1 cut(s) 178
NdeII GATC 5 cut(s) 115, 125, 212, 275, 487
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 1 cut(s) 214
NmeAIII GCCGAG 1 cut(s) 81
PfeI GAWTC 2 cut(s) 4, 167
PflFI GACNNNGTC 1 cut(s) 74
PkrI GCNGC 3 cut(s) 98, 258, 503
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PshBI ATTAAT 1 cut(s) 305
Psp124BI GAGCTC 1 cut(s) 339
Psp6I CCWGG 4 cut(s) 65, 150, 477, 513
PspGI CCWGG 4 cut(s) 65, 150, 477, 513
PspN4I GGNNCC 1 cut(s) 214
PspPI GGNCC 1 cut(s) 429
PstI CTGCAG 1 cut(s) 503
PsuI RGATCY 2 cut(s) 125, 212
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 1 cut(s) 241
RsaNI GTAC 1 cut(s) 240
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 3 cut(s) 97, 257, 502
Sau3AI GATC 5 cut(s) 115, 125, 212, 275, 487
Sau96I GGNCC 1 cut(s) 429
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 5 cut(s) 67, 152, 178, 479, 515
SduI GDGCHC 1 cut(s) 339
SetI ASST 7 cut(s) 43, 59, 94, 298, 339, 370, 382
SfaNI GCATC 1 cut(s) 513
SfcI CTRYAG 1 cut(s) 499
SinI GGWCC 1 cut(s) 429
Sse9I AATT 3 cut(s) 230, 306, 433
SsiI CCGC 3 cut(s) 97, 155, 273
SspI AATATT 1 cut(s) 199
SspMI CTAG 1 cut(s) 426
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 5 cut(s) 65, 150, 176, 477, 513
TaaI ACNGT 2 cut(s) 11, 357
TaqI TCGA 3 cut(s) 319, 462, 490
TasI AATT 3 cut(s) 230, 306, 433
TatI WGTACW 1 cut(s) 239
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 2 cut(s) 4, 167
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 3 cut(s) 14, 360, 459
TseI GCWGC 2 cut(s) 256, 501
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 3 cut(s) 14, 360, 459
Tth111I GACNNNGTC 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 429
VspI ATTAAT 1 cut(s) 305
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.