Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Reverse (-)

31632048 .. 31645191

Gene Structure 13144 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g33391.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3270 bp

ATGGGGAAGACAATACAGTTACATGGATTCCCTTCAGGTCAAACTGCCGAAAATATTAAGGAATTTTTGGAGAAACATACAGGAGAAGGAAGTGTTTTTGCTCTTAAATTACGGCCGGCCAAAAGTGGGGGAAGATTCTATGCTATTATACAGTTTCAGTCCATTAGTCAAGCTGAGAAAATCATCTCTTTGGCTAACGCTAGGCTGTGGTATGGGAGATCCTATCTGAATGCTCGTCCAGCCCATGATATTGTACCAAAGCCAAGAACTTTTTTGCATAGCTTGCGAGATTTAGTATTGCATGTTGGCTGCCAGGTTGCAAAAGATAAGTTTTCTGTTTTGTGGGGAACGGCGGAAGTTTCAGTGGACTTTGGGTTTGGAATGCGGAACTTGCAATTCCGGCTGTTATATCGTCAGAAGTTATATGGAAATCCTCGCATCTACCAACAAGAAGTATGTTCTTCTGGAGGAAATGGATACGAAGATATGAACTATTTTAGGGATCCTGATGACTGGGTTCGGACTACTGATTTCACTCCAGGAAGCTGTATCGGGCAGTCATCTTCATTATGTTTGGTGCTCACTTCTGATGGAGAACTTCCTGATTTTGAAGAAAATTTTGCTTATTATAAGAAGACTGATGGGCAGTTTATCTTGGAGAATGGCTCAACTTTTTCTCGCAACCGAGATTTGGTCCCTATTGTTGGTTCTCCTGCAGGGATTGATTTGCCATATGATATCTTATTCAAAATAAATTTGCTGGTTCAACAGGGTTGTCTATCAGGGCCAACGCTTGATAATAATTTTTATAGGCTGGTGGATCCACGAAGAATAAGCCGTCCCTGTATAGAGTTTGCCCTAGATAAATTATCTAACTTGAAGGAGAGCTGTTATGAACCTGCAGTGTGGCTTAATGAGGAGTACCGGAAGTACATGATGTCTAAGAAACCTCCGAAGCCAGCACTTATTTCCTTAGATTCTGGATTGGTATATGTACGCAGGGTCCAAATAACACCATCTAAAGTGTATTTTTCTGGTCCTGAGGTTAATGTCTCAAATCGCATACTACGCCAGTATTCCAGTGATATCGATAACTTTCTTCGTGTTTCATTTGTTGATGAGGAGATGGAAAAACTTTATTCAACAGATTTATCCCCCCGTACATCTACTAGGAAAACTCGCATCTACAACAGAGTTCTTTCTGTTCTCAGAGACGGCATAGTAGTTGGGAAAAAGAAATTTGAGTTTCTTGCATTCTCATCAAGTCAATTGCGAGAGAATTCACTGTGGATGTTTGCTCCAACTGATGAAGACAGTGCTGATACAATAAGAGCAGGTATGGGGGATTTTCGTCAAATAAGAAATGTGGCAAAGTATGCTGCCAGATTGGGTCAATCTTTTGGCTCTTCTACTGAAACTTTAAGTGTTGGCAGGAATGAAATGGAAGTTATTCCTGATGTAACCGTTAATAGTTGTGGAATCAAATATGTTTTCTCAGATGGGATTGGGAAAATATCAGCTGATTTTGCAGAGAGAGTAGCCAAAAAATGTGGCTATAAAGCTTTCACACCATCTGCATTTCAAATTCGATATGGGGGTTACAAAGGTGTTGTAGCTGTTGATCCAACCTCGTCAATGAAGTTATCACTGAGAAAGAGCATGTCCAAGTATGAATCTGAAAACACAAAGCTAGATGTTCTAGCATGTAGCAAGTATCAGCCTTGTTTTTTGAATCGTCAGTTGATAACTCTTCTGTCTACACTTGAAGTGCAAGATCATGTTTTCTTGAAAAAGCAAAGAGCAGCTGTACAACAACTTAATGCCATATTAACTGACCCATTAAAAGCAGAGGAGGCTCTTGATTTGATGTCTGCGGGTGAGAGTACAAATGTCCTCAAGGAACTTCTCATGTGTGGTTATAAACCTAATGTTGAACCATTCCTTTCAATGATGCTCCAAACATTTCGAGCATCAAAGTTGTTGGAATTGCGGACCAAGACAAGAATCTTTATCCCAGATGGAAGAGCAATGATGGGATGTCTAGACGAAACAAGAACCTTGGAATATGGGCAGGTGTTTGTGCAATTCTCTGGCAAAAGACGAAGGCAGTTATTTGATGATATATGCATACATAGTTCCTTAGACAGCTGTTTCATTATTGAGGGAAAGGTGGTTGTTGCAAAGAATCCATGTTTACACCCTGGTGATGTGCGTGTTTTAAGGGCTGTGAATGTGCCGGACTTACACCATATGGTGGACTGTGTGGTATTTCCACAAAAAGGACACAGGCCTCACCCAAATGAATGTTCTGGAAGTGATCTTGATGGAGATATTTACTTTGTTTGCTGGGACCCTGAGTTGATTCCTCCCAAGCAAATCCAACCTATGGATTATACCCCAGCACCACCTATAGAGATGGATCAGGATGTCACAATTGAGGAAGTAGAAGAGTATTTTGTGAACTACATAGTCAATGATAGTTTAGGGATCATCGCCAATGCCCACACTGTTTTCGCTGACAGTAAGCTTGATAAAGCAATGAGCCATCCGTGTTTACAGCTTGCAAAGCTATTTTCAATTGCTGTAGACTTTCCAAAAACTGGTGTACCAGCTGAGATACCACCAGAACTGTATGCCAAGGAATATCCAGATTTCATGGAGAAGCCCGACAAACGCACCTATATTTCACACAATGTGATTGGACAGCTTTTTCGTGAGGTGAAGGACATTGCACCTCATGACAGCTCTATTGCATCCTTCACTCGTCAAGTGGCGACAAAATGCTATGACCCTGACATGGAAGTTGATGGCTTTGAGGATTACCTTGAAGATGCTTTCTATCACAAAGGCAATTATGATTACAAATTGGGAAACATGTTGGATTACTATGGGATCAAGACAGAGGCTGAAATCCTGAGTGGGAGTATTATGACATTGTCGAAATCTTTCACCAAGAGGCGGGATGCAGATGCCATAAACGTGGCTGTGAAGTCATTGAGGAAGGAAGCTAGGGCCTGGTTCAATGAGAAAGACACTGGAATAGATTCTGGAGATGATGATGTCTATGCAAAAGCTTCAGCTTGGTACCATGTCACGTATCATCCCAATTATTTTGGTTTGTACAATGAGGGAATGAATCGTGCCCATTTTATCAGCTTTCCGTGGTGTGTTTTTGACAAGCTGGGTCACATAAAAAAGGAAAAAGTAAACCAGGCTCGACTAATTTCATCTCTGGAAAATCGGTTGAGGTATGGCTTGCGTTTTTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

1090

Amino Acids

123.54

Weight (kDa)

7.04

Isoelectric Point (pI)

36.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_RdRP1_2	PF26250	4 - 79	1.9e-16	RdRP1/2, RRM domain
RDR6_2nd	PF24577	95 - 240	3.1e-06	RNA-dependent RNA polymerase 6 second domain
PH_RDR2	PF24823	100 - 247	5.4e-39	RDR2 PH-like domain
RdRP_helical	PF26252	269 - 345	1.7e-10	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	367 - 937	8e-197	RNA dependent RNA polymerase
RdRP_head	PF26253	959 - 1105	1.1e-46	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	630, 1920
AarI	CACCTGC	1 cut(s)	2062
Acc36I	ACCTGC	3 cut(s)	907, 1325, 2062
Acc65I	GGTACC	1 cut(s)	3084
AccB1I	GGYRCC	1 cut(s)	3084
AccB7I	CCANNNNNTGG	3 cut(s)	2254, 2386, 2600
AccI	GTMKAC	2 cut(s)	1757, 2586
AciI	CCGC	5 cut(s)	353, 385, 1874, 1990, 2959
AclWI	GGATC	9 cut(s)	213, 497, 510, 815, 828, 1616, 2427, 2495, 2900
AcoI	YGGCCR	2 cut(s)	113, 117
AcsI	RAATTY	6 cut(s)	62, 616, 754, 1238, 1279, 1584
AcuI	CTGAAG	2 cut(s)	18, 3060
AdeI	CACNNNGTG	1 cut(s)	2695
AfiI	CCNNNNNNNGG	9 cut(s)	126, 691, 704, 2254, 2279, 2386, 2600, 2797, 2958
AflIII	ACRYGT	1 cut(s)	2874
AjnI	CCWGG	5 cut(s)	312, 538, 2200, 3014, 3210
AjuI	GAANNNNNNNTTGG	2 cut(s)	2290, 2322
AleI	CACNNNNGTG	1 cut(s)	2202
Alw21I	GWGCWC	1 cut(s)	582
Alw26I	GTCTC	2 cut(s)	1057, 1206
AlwI	GGATC	9 cut(s)	213, 497, 510, 815, 828, 1616, 2427, 2495, 2900
AlwNI	CAGNNNCTG	1 cut(s)	2906
AoxI	GGCC	5 cut(s)	113, 117, 785, 2288, 3012
ApeKI	GCWGC	3 cut(s)	309, 1379, 1802
ApoI	RAATTY	6 cut(s)	62, 616, 754, 1238, 1279, 1584
ArsI	GACNNNNNNTTYG	4 cut(s)	359, 391, 2694, 2726
Asp700I	GAANNNNTTC	3 cut(s)	1449, 2945, 3043
Asp718I	GGTACC	1 cut(s)	3084
AspS9I	GGNCC	7 cut(s)	694, 785, 1003, 1037, 1992, 2350, 3012
AsuHPI	GGTGA	5 cut(s)	1889, 2216, 2285, 2731, 2941
AvaII	GGWCC	5 cut(s)	694, 1003, 1037, 1992, 2350
AxyI	CCTNAGG	1 cut(s)	1041
BaeGI	GKGCMC	1 cut(s)	3145
BaeI	ACNNNNGTAYC	2 cut(s)	2589, 2622
BamHI	GGATCC	2 cut(s)	502, 820
BanI	GGYRCC	1 cut(s)	3084
BbsI	GAAGAC	3 cut(s)	14, 641, 1317
Bbv12I	GWGCWC	1 cut(s)	582
BbvI	GCAGC	3 cut(s)	296, 1366, 1814
BceAI	ACGGC	4 cut(s)	128, 366, 822, 1231
BciT130I	CCWGG	5 cut(s)	314, 540, 2202, 3016, 3212
BciVI	GTATCC	1 cut(s)	470
BcoDI	GTCTC	2 cut(s)	1057, 1206
BfaI	CTAG	7 cut(s)	201, 860, 1170, 1691, 1700, 2042, 3009
BfmI	CTRYAG	4 cut(s)	714, 900, 2409, 2583
BfuAI	ACCTGC	3 cut(s)	907, 1325, 2062
BfuI	GTATCC	1 cut(s)	470
BisI	GCNGC	3 cut(s)	310, 1380, 1803
BlsI	GCNGC	3 cut(s)	311, 1381, 1804
Bme1390I	CCNGG	5 cut(s)	314, 540, 2202, 3016, 3212
Bme18I	GGWCC	5 cut(s)	694, 1003, 1037, 1992, 2350
BmgT120I	GGNCC	7 cut(s)	694, 785, 1003, 1037, 1992, 2350, 3012
BmiI	GGNNCC	7 cut(s)	504, 696, 822, 1004, 2351, 2352, 3086
BmrFI	CCNGG	5 cut(s)	314, 540, 2202, 3016, 3212
BmrI	ACTGGG	1 cut(s)	523
BmsI	GCATC	8 cut(s)	447, 1191, 1941, 1979, 2762, 2821, 2953, 2959
BmuI	ACTGGG	1 cut(s)	523
BpiI	GAAGAC	3 cut(s)	14, 641, 1317
BplI	GAGNNNNNCTC	2 cut(s)	650, 682
BpmI	CTGGAG	3 cut(s)	486, 522, 3069
BpuEI	CTTGAG	1 cut(s)	1880
Bsa29I	ATCGAT	1 cut(s)	1089
BsaAI	YACGTR	1 cut(s)	3096
BsaJI	CCNNGG	4 cut(s)	2058, 2200, 2637, 3161
BsaWI	WCCGGW	1 cut(s)	924
Bsc4I	CCNNNNNNNGG	9 cut(s)	126, 691, 704, 2254, 2279, 2386, 2600, 2797, 2958
Bse118I	RCCGGY	1 cut(s)	115
Bse1I	ACTGG	5 cut(s)	518, 1072, 1080, 2605, 3040
Bse21I	CCTNAGG	1 cut(s)	1041
Bse3DI	GCAATG	3 cut(s)	2034, 2544, 2727
BseBI	CCWGG	5 cut(s)	314, 540, 2202, 3016, 3212
BseCI	ATCGAT	1 cut(s)	1089
BseDI	CCNNGG	4 cut(s)	2058, 2200, 2637, 3161
BseGI	GGATG	7 cut(s)	1296, 2042, 2431, 2545, 2753, 2968, 3100
BseLI	CCNNNNNNNGG	9 cut(s)	126, 691, 704, 2254, 2279, 2386, 2600, 2797, 2958
BseMI	GCAATG	3 cut(s)	2034, 2544, 2727
BseMII	CTCAG	8 cut(s)	165, 1032, 1222, 1509, 1640, 2346, 2604, 2906
BseNI	ACTGG	5 cut(s)	518, 1072, 1080, 2605, 3040
BseRI	GAGGAG	3 cut(s)	932, 1136, 1865
BseSI	GKGCMC	1 cut(s)	3145
BseX3I	CGGCCG	1 cut(s)	113
BseXI	GCAGC	3 cut(s)	296, 1366, 1814
BseYI	CCCAGC	3 cut(s)	2346, 2398, 3181
Bsh1285I	CGRYCG	1 cut(s)	116
BshFI	GGCC	5 cut(s)	115, 119, 787, 2290, 3014
BshNI	GGYRCC	1 cut(s)	3084
BshVI	ATCGAT	1 cut(s)	1089
BsiEI	CGRYCG	1 cut(s)	116
BsiHKAI	GWGCWC	1 cut(s)	582
BsiSI	CCGG	4 cut(s)	116, 400, 925, 2237
BslFI	GGGAC	3 cut(s)	680, 825, 2363
BslI	CCNNNNNNNGG	9 cut(s)	126, 691, 704, 2254, 2279, 2386, 2600, 2797, 2958
BsmAI	GTCTC	2 cut(s)	1057, 1206
BsmBI	CGTCTC	1 cut(s)	1206
BsmFI	GGGAC	3 cut(s)	680, 825, 2363
BsmI	GAATGC	3 cut(s)	235, 387, 1253
BsnI	GGCC	5 cut(s)	115, 119, 787, 2290, 3014
Bsp1286I	GDGCHC	2 cut(s)	582, 3145
Bsp1407I	TGTACA	2 cut(s)	1807, 3120
Bsp143I	GATC	9 cut(s)	218, 502, 820, 1621, 1774, 2317, 2419, 2487, 2892
BspACI	CCGC	5 cut(s)	353, 385, 1874, 1990, 2959
BspANI	GGCC	5 cut(s)	115, 119, 787, 2290, 3014
BspCNI	CTCAG	8 cut(s)	166, 1033, 1221, 1508, 1641, 2347, 2605, 2907
BspDI	ATCGAT	1 cut(s)	1089
BspHI	TCATGA	1 cut(s)	2737
BspLI	GGNNCC	7 cut(s)	504, 696, 822, 1004, 2351, 2352, 3086
BspMAI	CTGCAG	2 cut(s)	718, 904
BspMI	ACCTGC	3 cut(s)	907, 1325, 2062
BspPI	GGATC	9 cut(s)	213, 497, 510, 815, 828, 1616, 2427, 2495, 2900
BspQI	GCTCTTC	2 cut(s)	1411, 2017
BspT107I	GGYRCC	1 cut(s)	3084
BsrDI	GCAATG	3 cut(s)	2034, 2544, 2727
BsrFI	RCCGGY	1 cut(s)	115
BsrGI	TGTACA	2 cut(s)	1807, 3120
BsrI	ACTGG	5 cut(s)	518, 1072, 1080, 2605, 3040
BssAI	RCCGGY	1 cut(s)	115
BssECI	CCNNGG	4 cut(s)	2058, 2200, 2637, 3161
BssMI	GATC	9 cut(s)	218, 502, 820, 1621, 1774, 2317, 2419, 2487, 2892
BssT1I	CCWWGG	2 cut(s)	2058, 2637
Bst2UI	CCWGG	5 cut(s)	314, 540, 2202, 3016, 3212
Bst4CI	ACNGT	9 cut(s)	18, 153, 1287, 1316, 1465, 2261, 2509, 2522, 2631
Bst6I	CTCTTC	4 cut(s)	1411, 1755, 2017, 2442
BstAPI	GCANNNNNTGC	2 cut(s)	283, 1376
BstAUI	TGTACA	2 cut(s)	1807, 3120
BstBAI	YACGTR	1 cut(s)	3096
BstC8I	GCNNGC	5 cut(s)	117, 284, 960, 2562, 3257
BstDSI	CCRYGG	1 cut(s)	3161
BstF5I	GGATG	7 cut(s)	1296, 2042, 2431, 2545, 2753, 2968, 3100
BstKTI	GATC	9 cut(s)	221, 505, 823, 1624, 1777, 2320, 2422, 2490, 2895
BstMAI	GTCTC	2 cut(s)	1057, 1206
BstMBI	GATC	9 cut(s)	218, 502, 820, 1621, 1774, 2317, 2419, 2487, 2892
BstMCI	CGRYCG	1 cut(s)	116
BstMWI	GCNNNNNNNGC	9 cut(s)	239, 283, 391, 400, 1068, 1376, 1526, 1853, 2566
BstNI	CCWGG	5 cut(s)	314, 540, 2202, 3016, 3212
BstNSI	RCATGY	4 cut(s)	305, 1663, 1707, 2878
BstSCI	CCNGG	5 cut(s)	312, 538, 2200, 3014, 3210
BstSFI	CTRYAG	4 cut(s)	714, 900, 2409, 2583
BstSLI	GKGCMC	1 cut(s)	3145
BstV1I	GCAGC	3 cut(s)	296, 1366, 1814
BstV2I	GAAGAC	3 cut(s)	14, 641, 1317
BstX2I	RGATCY	3 cut(s)	218, 502, 820
BstXI	CCANNNNNNTGG	1 cut(s)	2980
BstYI	RGATCY	3 cut(s)	218, 502, 820
BstZI	CGGCCG	1 cut(s)	113
Bsu15I	ATCGAT	1 cut(s)	1089
Bsu36I	CCTNAGG	1 cut(s)	1041
BsuI	GTATCC	1 cut(s)	470
BsuRI	GGCC	5 cut(s)	115, 119, 787, 2290, 3014
BsuTUI	ATCGAT	1 cut(s)	1089
BtgI	CCRYGG	1 cut(s)	3161
BtgZI	GCGATG	1 cut(s)	2476
BtsCI	GGATG	7 cut(s)	1296, 2042, 2431, 2545, 2753, 2968, 3100
BtsI	GCAGTG	1 cut(s)	909
BtsIMutI	CAGTG	8 cut(s)	369, 909, 1087, 1283, 1321, 1646, 2505, 3033
BveI	ACCTGC	3 cut(s)	907, 1325, 2062
Cac8I	GCNNGC	5 cut(s)	117, 284, 960, 2562, 3257
CaiI	CAGNNNCTG	1 cut(s)	2906
CciI	TCATGA	1 cut(s)	2737
Cfr10I	RCCGGY	1 cut(s)	115
Cfr13I	GGNCC	7 cut(s)	694, 785, 1003, 1037, 1992, 2350, 3012
ClaI	ATCGAT	1 cut(s)	1089
CspCI	CAANNNNNGTGG	2 cut(s)	1531, 1566
DpnI	GATC	9 cut(s)	220, 504, 822, 1623, 1776, 2319, 2421, 2489, 2894
DpnII	GATC	9 cut(s)	218, 502, 820, 1621, 1774, 2317, 2419, 2487, 2892
DraIII	CACNNNGTG	1 cut(s)	2695
EaeI	YGGCCR	2 cut(s)	113, 117
EagI	CGGCCG	1 cut(s)	113
Eam1104I	CTCTTC	4 cut(s)	1411, 1755, 2017, 2442
EarI	CTCTTC	4 cut(s)	1411, 1755, 2017, 2442
EciI	GGCGGA	1 cut(s)	368
EclXI	CGGCCG	1 cut(s)	113
Eco130I	CCWWGG	2 cut(s)	2058, 2637
Eco147I	AGGCCT	1 cut(s)	2290
Eco32I	GATATC	2 cut(s)	739, 1087
Eco47I	GGWCC	5 cut(s)	694, 1003, 1037, 1992, 2350
Eco52I	CGGCCG	1 cut(s)	113
Eco57I	CTGAAG	2 cut(s)	18, 3060
Eco81I	CCTNAGG	1 cut(s)	1041
EcoO109I	RGGNCCY	2 cut(s)	2350, 3012
EcoRI	GAATTC	1 cut(s)	1279
EcoRII	CCWGG	5 cut(s)	312, 538, 2200, 3014, 3210
EcoRV	GATATC	2 cut(s)	739, 1087
EcoT14I	CCWWGG	2 cut(s)	2058, 2637
EcoT22I	ATGCAT	1 cut(s)	2129
ErhI	CCWWGG	2 cut(s)	2058, 2637
Esp3I	CGTCTC	1 cut(s)	1206
FaqI	GGGAC	3 cut(s)	680, 825, 2363
FauI	CCCGC	2 cut(s)	1867, 2952
FauNDI	CATATG	2 cut(s)	733, 2250
FblI	GTMKAC	2 cut(s)	1757, 2586
Fnu4HI	GCNGC	3 cut(s)	310, 1380, 1803
FokI	GGATG	7 cut(s)	1303, 2049, 2438, 2532, 2740, 2975, 3087
FseI	GGCCGGCC	1 cut(s)	119
Fsp4HI	GCNGC	3 cut(s)	310, 1380, 1803
FspBI	CTAG	7 cut(s)	201, 860, 1170, 1691, 1700, 2042, 3009
GluI	GCNGC	3 cut(s)	310, 1380, 1803
GsaI	CCCAGC	3 cut(s)	2350, 2402, 3185
GsuI	CTGGAG	3 cut(s)	486, 522, 3069
HaeIII	GGCC	5 cut(s)	115, 119, 787, 2290, 3014
HapII	CCGG	4 cut(s)	116, 400, 925, 2237
HindIII	AAGCTT	3 cut(s)	1560, 2525, 3072
HpaII	CCGG	4 cut(s)	116, 400, 925, 2237
HphI	GGTGA	5 cut(s)	1889, 2216, 2285, 2731, 2941
Hpy166II	GTNNAC	9 cut(s)	367, 1758, 2195, 2257, 2461, 2555, 2587, 2606, 3208
Hpy188I	TCNGA	8 cut(s)	228, 417, 522, 589, 954, 1211, 1498, 1678
Hpy8I	GTNNAC	9 cut(s)	367, 1758, 2195, 2257, 2461, 2555, 2587, 2606, 3208
HpyAV	CCTTC	7 cut(s)	42, 80, 874, 2097, 2716, 2767, 2995
HpyCH4III	ACNGT	9 cut(s)	18, 153, 1287, 1316, 1465, 2261, 2509, 2522, 2631
HpyCH4IV	ACGT	2 cut(s)	2979, 3095
HpyF10VI	GCNNNNNNNGC	9 cut(s)	239, 283, 391, 400, 1068, 1376, 1526, 1853, 2566
HpySE526I	ACGT	2 cut(s)	2979, 3095
KflI	GGGWCCC	1 cut(s)	2350
KpnI	GGTACC	1 cut(s)	3088
KroI	GCCGGC	1 cut(s)	115
KroNI	GCCGGC	1 cut(s)	117
Kzo9I	GATC	9 cut(s)	218, 502, 820, 1621, 1774, 2317, 2419, 2487, 2892
LguI	GCTCTTC	2 cut(s)	1411, 2017
LmnI	GCTCC	2 cut(s)	1303, 1959
Lsp1109I	GCAGC	3 cut(s)	296, 1366, 1814
LweI	GCATC	8 cut(s)	447, 1191, 1941, 1979, 2762, 2821, 2953, 2959
MaeI	CTAG	7 cut(s)	201, 860, 1170, 1691, 1700, 2042, 3009
MaeII	ACGT	2 cut(s)	2979, 3095
MaeIII	GTNAC	6 cut(s)	18, 1459, 1598, 2428, 3091, 3185
MalI	GATC	9 cut(s)	220, 504, 822, 1623, 1776, 2319, 2421, 2489, 2894
MboI	GATC	9 cut(s)	218, 502, 820, 1621, 1774, 2317, 2419, 2487, 2892
MfeI	CAATTG	3 cut(s)	1268, 2433, 2577
MflI	RGATCY	3 cut(s)	218, 502, 820
MhlI	GDGCHC	2 cut(s)	582, 3145
MmeI	TCCRAC	5 cut(s)	1325, 1649, 1962, 2404, 2859
Mph1103I	ATGCAT	1 cut(s)	2129
MroNI	GCCGGC	1 cut(s)	115
MroXI	GAANNNNTTC	3 cut(s)	1449, 2945, 3043
MslI	CAYNNNNRTG	3 cut(s)	2202, 2298, 2978
MspA1I	CMGCKG	4 cut(s)	1520, 1805, 2148, 2612
MspI	CCGG	4 cut(s)	116, 400, 925, 2237
MspR9I	CCNGG	5 cut(s)	314, 540, 2202, 3016, 3212
MunI	CAATTG	3 cut(s)	1268, 2433, 2577
Mva1269I	GAATGC	3 cut(s)	235, 387, 1253
MvaI	CCWGG	5 cut(s)	314, 540, 2202, 3016, 3212
MwoI	GCNNNNNNNGC	9 cut(s)	239, 283, 391, 400, 1068, 1376, 1526, 1853, 2566
NaeI	GCCGGC	1 cut(s)	117
NdeI	CATATG	2 cut(s)	733, 2250
NdeII	GATC	9 cut(s)	218, 502, 820, 1621, 1774, 2317, 2419, 2487, 2892
NgoMIV	GCCGGC	1 cut(s)	115
NlaIV	GGNNCC	7 cut(s)	504, 696, 822, 1004, 2351, 2352, 3086
NmuCI	GTSAC	3 cut(s)	2428, 3091, 3185
NsiI	ATGCAT	1 cut(s)	2129
NspI	RCATGY	4 cut(s)	305, 1663, 1707, 2878
OliI	CACNNNNGTG	1 cut(s)	2202
PagI	TCATGA	1 cut(s)	2737
PaqCI	CACCTGC	1 cut(s)	2062
PceI	AGGCCT	1 cut(s)	2290
PciI	ACATGT	1 cut(s)	2874
PciSI	GCTCTTC	2 cut(s)	1411, 2017
PctI	GAATGC	3 cut(s)	235, 387, 1253
PdiI	GCCGGC	1 cut(s)	117
PdmI	GAANNNNTTC	3 cut(s)	1449, 2945, 3043
PflFI	GACNNNGTC	1 cut(s)	2935
PflMI	CCANNNNNTGG	3 cut(s)	2254, 2386, 2600
PfoI	TCCNGGA	1 cut(s)	538
PkrI	GCNGC	3 cut(s)	311, 1381, 1804
Ppu21I	YACGTR	1 cut(s)	3096
PpuMI	RGGWCCY	1 cut(s)	2350
PscI	ACATGT	1 cut(s)	2874
PsiI	TTATAA	2 cut(s)	630, 1920
Psp5II	RGGWCCY	1 cut(s)	2350
Psp6I	CCWGG	5 cut(s)	312, 538, 2200, 3014, 3210
PspFI	CCCAGC	3 cut(s)	2346, 2398, 3181
PspGI	CCWGG	5 cut(s)	312, 538, 2200, 3014, 3210
PspN4I	GGNNCC	7 cut(s)	504, 696, 822, 1004, 2351, 2352, 3086
PspPI	GGNCC	7 cut(s)	694, 785, 1003, 1037, 1992, 2350, 3012
PspPPI	RGGWCCY	1 cut(s)	2350
PstI	CTGCAG	2 cut(s)	718, 904
PstNI	CAGNNNCTG	1 cut(s)	2906
PsuI	RGATCY	3 cut(s)	218, 502, 820
PsyI	GACNNNGTC	1 cut(s)	2935
PvuII	CAGCTG	4 cut(s)	1520, 1805, 2148, 2612
RigI	GGCCGGCC	1 cut(s)	119
RseI	CAYNNNNRTG	3 cut(s)	2202, 2298, 2978
SapI	GCTCTTC	2 cut(s)	1411, 2017
SatI	GCNGC	3 cut(s)	310, 1380, 1803
Sau3AI	GATC	9 cut(s)	218, 502, 820, 1621, 1774, 2317, 2419, 2487, 2892
Sau96I	GGNCC	7 cut(s)	694, 785, 1003, 1037, 1992, 2350, 3012
SbfI	CCTGCAGG	1 cut(s)	718
ScrFI	CCNGG	5 cut(s)	314, 540, 2202, 3016, 3212
SdaI	CCTGCAGG	1 cut(s)	718
SduI	GDGCHC	2 cut(s)	582, 3145
SfaNI	GCATC	8 cut(s)	447, 1191, 1941, 1979, 2762, 2821, 2953, 2959
SfcI	CTRYAG	4 cut(s)	714, 900, 2409, 2583
SinI	GGWCC	5 cut(s)	694, 1003, 1037, 1992, 2350
SmiMI	CAYNNNNRTG	3 cut(s)	2202, 2298, 2978
SmlI	CTYRAG	1 cut(s)	1895
SmoI	CTYRAG	1 cut(s)	1895
Sse8387I	CCTGCAGG	1 cut(s)	718
SseBI	AGGCCT	1 cut(s)	2290
SsiI	CCGC	5 cut(s)	353, 385, 1874, 1990, 2959
SspI	AATATT	1 cut(s)	55
SspMI	CTAG	7 cut(s)	201, 860, 1170, 1691, 1700, 2042, 3009
StuI	AGGCCT	1 cut(s)	2290
StyD4I	CCNGG	5 cut(s)	312, 538, 2200, 3014, 3210
StyI	CCWWGG	2 cut(s)	2058, 2637
TaaI	ACNGT	9 cut(s)	18, 153, 1287, 1316, 1465, 2261, 2509, 2522, 2631
TaiI	ACGT	2 cut(s)	2982, 3098
TaqI	TCGA	5 cut(s)	1089, 1588, 1966, 2939, 3217
TatI	WGTACW	4 cut(s)	930, 1807, 1883, 3120
TscAI	CASTG	8 cut(s)	369, 909, 1087, 1290, 1321, 1653, 2512, 3040
TseFI	GTSAC	3 cut(s)	2428, 3091, 3185
TseI	GCWGC	3 cut(s)	309, 1379, 1802
Tsp45I	GTSAC	3 cut(s)	2428, 3091, 3185
TspGWI	ACGGA	2 cut(s)	2538, 3150
TspRI	CASTG	8 cut(s)	369, 909, 1087, 1290, 1321, 1653, 2512, 3040
Tth111I	GACNNNGTC	1 cut(s)	2935
Van91I	CCANNNNNTGG	3 cut(s)	2254, 2386, 2600
VpaK11BI	GGWCC	5 cut(s)	694, 1003, 1037, 1992, 2350
XapI	RAATTY	6 cut(s)	62, 616, 754, 1238, 1279, 1584
XbaI	TCTAGA	1 cut(s)	2041
XceI	RCATGY	4 cut(s)	305, 1663, 1707, 2878
XmiI	GTMKAC	2 cut(s)	1757, 2586
XmnI	GAANNNNTTC	3 cut(s)	1449, 2945, 3043
XspI	CTAG	7 cut(s)	201, 860, 1170, 1691, 1700, 2042, 3009
Zsp2I	ATGCAT	1 cut(s)	2129

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.