Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Reverse (-)

1270274 .. 1275763

Gene Structure 5490 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1017200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3345 bp

ATGGGTAAGACGATACAGTTGTTTGGATTCCCTTCTGGTGCATCTGCAGAAGCTGTTTTTGAATGGCTGGAGAGAAAAACTGGAAAAGGATCTGTTTATGCTATGAGATACAGACCAGCCAACAATTCAAGGAGATATTATGCTATTATTCAGTTTGAAACCGCCAATGATGCTGAGTTTATTAATTCCTTGGCCGACACTGGAAACCTGTGGTATGAGAGAAGCTATCTGAAGTCCCGGAAGATGGATCATGATATTGTACCAAAGCCAAGAACCGCTTTGCACAGTCTGCAAAATCTAGAATTGTATTTTGGTTGCAAGACCGGAAAAGAAGAGCTATCTTATTTTTGGTGTTCGGACAATGTTTCAGTGGACTTTGGGATTAGAATACAGAAATTAATTTGTCTTCTGAAATATGATGAGGTGGAATATAAGCTTGAACTTTCCTATGAGAACATTTGGCAGACTGAGTTGTATAGTCCACGTGGTCACACTGCTAAGAAACTTCTGTTTCAGTTATTGGGAGCGCCTCGGATTTTCGAGAAAGAAGCAGACTCTTCAGGACAAGCATATGAAGACCCTTTTCAGAACTATTGCAGGGAAACCCCTGATGACCAATGGATCCGAACAACTGATTTCACTCCATCGTGCGCTATTGGGCAGTCTTCTGTATTATGCTTGGAGCTTCCTTATGGTCTCCAACTTCCAGATTTTGGAGAAAACTTTCCCGGGTACAAAGGGATTGTAAGGGACCTTGTCACGGAGTTATGTCCCCCTTTTTCTTGCTCGGATCTGGGAGCCATGGTTTGCCCTCCTGAATGTATTGGTGTTCCATATGATATCTTGTTTAAGGTAAACTCCCTGGTTCAGCAAGGGTGGCTTCCTGGGCCAAAACTTGGTGATAATTTTTTTGAGTTGGTTGATGCACGCAAAAGAGACCTTGCATGCATAGAGTATGCTCTAGATAAATTATACAATTTAAGAGATAGCTGTTATGAACCTTCAATGTGGCTTGAGGAGCAGTATATGAAGTACCGCAGATTGAAGAAAACTCCAAAATCACCAGTAATATCCTTAGATTCTGGGTTGGTGTATGTTCGTAGAGTCCAGATCACATCATGTCAAATACATTATTGTGGTCCTGAAGTCAATATCTCAAATCGTGTACTACGCAATTACCCAATGGATACTGATAACTTCATTCGTGTTTCATTTGTTGGAGAGGAGCTTGCAAAAATTTATTCGGCAGATTTGTCTCCTCGTAAGTCCACTACAAATCAGGATAGGAAAACCAAATTCTACATGAGGATTCTTTCTATTCTCAGAAATGGCATAGTAATTGGTGAAAAGAAATATGAATTTCTTGCATTCTCGTCGAGCCAATTGCGGGAGAATTCTATGTGGATGTTTGCTTCAAGGTGGGGACTTACAGCTGATGATATAAGAGAGTGGATGGGAGATTTTTGTCATATAAAAAATGTGGCAAAGTACGCTGCTAGATTGGGTCAATCTTTTGGTTCGTCCACTGAAACTTTGACTGTGGCCAAACATGAAATTGAAGTTATTCCCGACATTGTCCATGATAAATATGTCTTCTCTGATGGGATTGGGAAAATATCTGCTGATTTTGCGAAGAAAGTGGCCTTAAAATGTGGCTATAAGGGGTTTACTCCATCTGCCTTTCAGATTCGATATGGTGGTTACAAAGGTGTGGTGGCCATTGATCCAACTTCATCAATGAAATTATCATTAAGGAAGAGTATGTCCAAGTATGAATCAGAAAACACAAAGTTGGATGTATTAGCATGTAGCAAGTATCAGCCTTGTTTTCTGAACCGCCAGTTGATAACTCTTTTATCTACCCTTGAGGTTCCAGATCTTGTTTTCATGAAAAAACAAAGGGCAGCTGTACAACAACTGAATGCTATATTGACTGACCCACTAAAAGCACAGGACGCTTTGGATTTGATGTCTCCAGGAGAGACTACTAACATCCTGAAGGAATTGCTCACATGTGGTTATAAACCTGATGTTGAACCATTCCTTTCAATGATGCTGCAAACATTTCGGGCATCAAAGTTGCTAGATTTGCGACTGAAAACAAGAATCTTTATTCCAGATGGACGAGCAATGATGGGATGTCTAGATGAAACCGGAACATTGGAATATGGAGAGGTGTTTGTGCAATTATCTGGTAACAGACATCCTGGAAGTGCCTCAAACCATATTGAAGGAAAGGTAGTTGTTGCGAAGAACCCTTGTTTACACCCTGGTGATGTACGTGTTTTAAAGGCTGTGAATGTGCCGGAGTTGCACCATATGGTGGATTGTGTAGTTTTTCCACAAAAAGGATCAAGGCCTCATCCAAATGAATGTTCAGGAAGTGATCTCGATGGAGATATCTACTTTGTCTGTTGGGACCAAGAATTGATTCCTCGGAGACAAATCCAACCTATGGATTATACCCCAGCACCAAGTATGGAATTGGATCATGACGTCACAATGGAGGAAGTTGAGGAATATTTTGTGAACTACATGATCAATGATAGCTTGGGCATAATTGCCAATGCCCACACTGTTTTTGCAGACAAAGATCCTTTAAAAGCAATGAGCGAACCATGTAAAGAGCTTGCAAAGCTGTTTTCAATTGCCGTCGACTTTCCAAAAACTGGTGTACCAGCTGTAATACCTGCACATCTATATGTCAAGGAATTTCCAGATTTCATGGAAAAACATGACAAGCCCACCTATCAATCATGCAATGTGATCGGAGCGCTATTTCAAGAGGTGAAAGACATTGCGCCTCATGACAGCTCTATCAGATCCTTCACTCGTCAAGTGGCAAAGCAGTCATACGACCCTGACATGGAAGTTGATGGCTTTGAGGATTACATTGAAGATGCTATCTATTACAAAGGCAATTATGATTACAAGTTGGGAAATCTGATGGACTATTATGGGATCAAGACAGAGGCTGAAATACTTAGTGGGAGTGTTATGAGAATGTCAAAATCTTTCACTAAGAGGCGGGATGCAGATTCTATAAACATGGCTGTGAGATCGTTGAGGAAGGAAGCTCGGGCATGGTTCAATGAGAAGGGAACAGGTCTAGATTCCGGAGCTGATGATGTATACGCAAAAGCTTCAGCTTGGTACCATGTCACATATCATCCTGATTATTTTGGCACATACAATGAGGGGCTGAATCGTGACCATTTCATCAGCTTTCCGTGGTGCGTTTATGACAAGCTCGTCGTCATCAAAAAAGATAAGGCAAGTATGAGAAGGTCTCTACACATGTCATCTCTAGAACGCCAATTCCGGAGTGGGTTGCATTTGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

1115

Amino Acids

127.0

Weight (kDa)

6.21

Isoelectric Point (pI)

41.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_RdRP1_2	PF26250	4 - 80	2.8e-13	RdRP1/2, RRM domain
PH_RDR2	PF24823	102 - 252	2.6e-43	RDR2 PH-like domain
RDR6_2nd	PF24577	142 - 226	4e-07	RNA-dependent RNA polymerase 6 second domain
RdRP_helical	PF26252	268 - 345	2.1e-09	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	371 - 930	1.5e-194	RNA dependent RNA polymerase
RdRP_head	PF26253	951 - 1094	1.5e-48	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2022
AasI	GACNNNNNNGTC	1 cut(s)	3251
AatII	GACGTC	1 cut(s)	2499
Acc36I	ACCTGC	1 cut(s)	2698
Acc65I	GGTACC	1 cut(s)	3153
AccB1I	GGYRCC	1 cut(s)	3153
AccB7I	CCANNNNNTGG	5 cut(s)	713, 896, 2323, 2455, 2669
AccI	GTMKAC	2 cut(s)	2655, 3132
AccIII	TCCGGA	2 cut(s)	3116, 3321
AciI	CCGC	6 cut(s)	162, 276, 1036, 1387, 1837, 3028
AcoI	YGGCCR	3 cut(s)	192, 1542, 1716
AcsI	RAATTY	5 cut(s)	1236, 1295, 1358, 1393, 2711
AcuI	CTGAAG	5 cut(s)	251, 543, 1164, 2018, 3129
AcvI	CACGTG	1 cut(s)	485
AcyI	GRCGYC	1 cut(s)	2496
AfaI	GTAC	9 cut(s)	261, 734, 1034, 1167, 1490, 1911, 2280, 2676, 3155
AfeI	AGCGCT	1 cut(s)	2775
AflIII	ACRYGT	3 cut(s)	2012, 2281, 3297
AjnI	CCWGG	5 cut(s)	861, 883, 1975, 2206, 2269
AjuI	GAANNNNNNNTTGG	6 cut(s)	294, 326, 2359, 2391, 2415, 2447
AleI	CACNNNNGTG	1 cut(s)	2271
AloI	GAACNNNNNNTCC	2 cut(s)	3303, 3335
Alw26I	GTCTC	7 cut(s)	701, 930, 1260, 1976, 1977, 2434, 3294
AlwNI	CAGNNNCTG	2 cut(s)	53, 2975
Ama87I	CYCGRG	2 cut(s)	728, 3078
Aor13HI	TCCGGA	2 cut(s)	3116, 3321
Aor51HI	AGCGCT	1 cut(s)	2775
AoxI	GGCC	6 cut(s)	192, 887, 1542, 1641, 1716, 2357
ApeKI	GCWGC	3 cut(s)	1493, 1904, 2056
ApoI	RAATTY	5 cut(s)	1236, 1295, 1358, 1393, 2711
AseI	ATTAAT	2 cut(s)	183, 398
Asp700I	GAANNNNTTC	3 cut(s)	723, 1563, 2430
Asp718I	GGTACC	1 cut(s)	3153
AspLEI	GCGC	4 cut(s)	529, 653, 2776, 2803
AspS9I	GGNCC	4 cut(s)	751, 887, 1139, 2419
AsuC2I	CCSGG	3 cut(s)	238, 729, 730
AsuHPI	GGTGA	5 cut(s)	911, 1053, 1355, 2285, 2800
AvaI	CYCGRG	2 cut(s)	728, 3078
AvaII	GGWCC	3 cut(s)	751, 1139, 2419
BaeI	ACNNNNGTAYC	3 cut(s)	38, 2658, 2691
BalI	TGGCCA	2 cut(s)	1544, 1718
BamHI	GGATCC	1 cut(s)	621
BanI	GGYRCC	1 cut(s)	3153
BbrPI	CACGTG	1 cut(s)	485
BbsI	GAAGAC	4 cut(s)	398, 582, 657, 1585
BbvI	GCAGC	3 cut(s)	1480, 1916, 2043
BceAI	ACGGC	1 cut(s)	2636
BcgI	CGANNNNNNTGC	8 cut(s)	1356, 1366, 1390, 1400, 2048, 2082, 2749, 2783
BciT130I	CCWGG	5 cut(s)	863, 885, 1977, 2208, 2271
BciVI	GTATCC	1 cut(s)	1180
BclI	TGATCA	1 cut(s)	2538
BcnI	CCSGG	3 cut(s)	238, 729, 730
BcoDI	GTCTC	7 cut(s)	701, 930, 1260, 1976, 1977, 2434, 3294
BfaI	CTAG	7 cut(s)	299, 962, 1497, 2084, 2144, 3110, 3308
BfmI	CTRYAG	1 cut(s)	45
BfoI	RGCGCY	2 cut(s)	530, 2777
BfuAI	ACCTGC	1 cut(s)	2698
BfuI	GTATCC	1 cut(s)	1180
BglII	AGATCT	1 cut(s)	1876
BisI	GCNGC	3 cut(s)	1494, 1905, 2057
BlsI	GCNGC	3 cut(s)	1495, 1906, 2058
Bme1390I	CCNGG	8 cut(s)	238, 729, 730, 863, 885, 1977, 2208, 2271
Bme18I	GGWCC	3 cut(s)	751, 1139, 2419
BmeT110I	CYCGRG	2 cut(s)	728, 3078
BmgT120I	GGNCC	4 cut(s)	751, 887, 1139, 2419
BmiI	GGNNCC	6 cut(s)	623, 752, 799, 1872, 2420, 3155
BmrFI	CCNGG	8 cut(s)	238, 729, 730, 863, 885, 1977, 2208, 2271
BmsI	GCATC	7 cut(s)	50, 160, 913, 2043, 2081, 2890, 3022
BpiI	GAAGAC	4 cut(s)	398, 582, 657, 1585
BplI	GAGNNNNNCTC	2 cut(s)	3274, 3306
BpmI	CTGGAG	2 cut(s)	89, 1959
BpuEI	CTTGAG	2 cut(s)	1034, 1886
BpuMI	CCSGG	3 cut(s)	238, 729, 730
BsaAI	YACGTR	2 cut(s)	485, 2282
BsaHI	GRCGYC	1 cut(s)	2496
BsaI	GGTCTC	3 cut(s)	701, 930, 3294
BsaJI	CCNNGG	9 cut(s)	189, 530, 728, 801, 861, 884, 2269, 2435, 3230
BsaWI	WCCGGW	4 cut(s)	323, 2153, 3116, 3321
BsaXI	ACNNNNNCTCC	2 cut(s)	789, 819
Bse1I	ACTGG	5 cut(s)	85, 205, 1064, 1840, 2674
Bse3DI	GCAATG	4 cut(s)	2136, 2613, 2767, 2796
BseAI	TCCGGA	2 cut(s)	3116, 3321
BseBI	CCWGG	5 cut(s)	863, 885, 1977, 2208, 2271
BseDI	CCNNGG	9 cut(s)	189, 530, 728, 801, 861, 884, 2269, 2435, 3230
BseGI	GGATG	9 cut(s)	1410, 1458, 1801, 1992, 2144, 2203, 2362, 3037, 3169
BseMI	GCAATG	4 cut(s)	2136, 2613, 2767, 2796
BseMII	CTCAG	3 cut(s)	165, 459, 1336
BseNI	ACTGG	5 cut(s)	85, 205, 1064, 1840, 2674
BseRI	GAGGAG	3 cut(s)	1031, 1238, 1248
BseXI	GCAGC	3 cut(s)	1480, 1916, 2043
BseYI	CCCAGC	1 cut(s)	2467
BsgI	GTGCAG	1 cut(s)	2676
BshFI	GGCC	6 cut(s)	194, 889, 1544, 1643, 1718, 2359
BshNI	GGYRCC	1 cut(s)	3153
BsiHKCI	CYCGRG	2 cut(s)	728, 3078
BsiSI	CCGG	7 cut(s)	238, 324, 729, 2154, 2306, 3117, 3322
BslFI	GGGAC	5 cut(s)	220, 756, 764, 1437, 2432
BsmAI	GTCTC	7 cut(s)	701, 930, 1260, 1976, 1977, 2434, 3294
BsmFI	GGGAC	5 cut(s)	220, 756, 764, 1437, 2432
BsmI	GAATGC	2 cut(s)	1367, 1927
BsnI	GGCC	6 cut(s)	194, 889, 1544, 1643, 1718, 2359
Bso31I	GGTCTC	3 cut(s)	701, 930, 3294
BsoBI	CYCGRG	2 cut(s)	728, 3078
Bsp13I	TCCGGA	2 cut(s)	3116, 3321
Bsp1407I	TGTACA	1 cut(s)	1909
Bsp19I	CCATGG	1 cut(s)	801
BspACI	CCGC	6 cut(s)	162, 276, 1036, 1387, 1837, 3028
BspANI	GGCC	6 cut(s)	194, 889, 1544, 1643, 1718, 2359
BspCNI	CTCAG	3 cut(s)	166, 460, 1335
BspEI	TCCGGA	2 cut(s)	3116, 3321
BspHI	TCATGA	4 cut(s)	250, 1887, 2491, 2806
BspLI	GGNNCC	6 cut(s)	623, 752, 799, 1872, 2420, 3155
BspMAI	CTGCAG	1 cut(s)	49
BspMI	ACCTGC	1 cut(s)	2698
BspQI	GCTCTTC	1 cut(s)	327
BspT107I	GGYRCC	1 cut(s)	3153
BspTNI	GGTCTC	3 cut(s)	701, 930, 3294
BsrDI	GCAATG	4 cut(s)	2136, 2613, 2767, 2796
BsrGI	TGTACA	1 cut(s)	1909
BsrI	ACTGG	5 cut(s)	85, 205, 1064, 1840, 2674
BssECI	CCNNGG	9 cut(s)	189, 530, 728, 801, 861, 884, 2269, 2435, 3230
BssNAI	GTATAC	1 cut(s)	3133
BssNI	GRCGYC	1 cut(s)	2496
BssT1I	CCWWGG	2 cut(s)	189, 801
Bst1107I	GTATAC	1 cut(s)	3133
Bst2UI	CCWGG	5 cut(s)	863, 885, 1977, 2208, 2271
Bst4CI	ACNGT	4 cut(s)	18, 287, 1540, 2578
Bst6I	CTCTTC	3 cut(s)	327, 562, 1751
BstACI	GRCGYC	1 cut(s)	2496
BstAPI	GCANNNNNTGC	1 cut(s)	289
BstAUI	TGTACA	1 cut(s)	1909
BstBAI	YACGTR	2 cut(s)	485, 2282
BstC8I	GCNNGC	4 cut(s)	928, 946, 1230, 2631
BstDEI	CTNAG	7 cut(s)	174, 468, 498, 1075, 1322, 2984, 3021
BstDSI	CCRYGG	2 cut(s)	801, 3230
BstF5I	GGATG	9 cut(s)	1410, 1458, 1801, 1992, 2144, 2203, 2362, 3037, 3169
BstH2I	RGCGCY	2 cut(s)	530, 2777
BstHHI	GCGC	4 cut(s)	529, 653, 2776, 2803
BstMAI	GTCTC	7 cut(s)	701, 930, 1260, 1976, 1977, 2434, 3294
BstNI	CCWGG	5 cut(s)	863, 885, 1977, 2208, 2271
BstNSI	RCATGY	4 cut(s)	948, 1809, 2016, 3301
BstSCI	CCNGG	8 cut(s)	236, 727, 728, 861, 883, 1975, 2206, 2269
BstSFI	CTRYAG	1 cut(s)	45
BstV1I	GCAGC	3 cut(s)	1480, 1916, 2043
BstV2I	GAAGAC	4 cut(s)	398, 582, 657, 1585
BstX2I	RGATCY	6 cut(s)	89, 621, 790, 1876, 2593, 2822
BstYI	RGATCY	6 cut(s)	89, 621, 790, 1876, 2593, 2822
BstZ17I	GTATAC	1 cut(s)	3133
BsuI	GTATCC	1 cut(s)	1180
BsuRI	GGCC	6 cut(s)	194, 889, 1544, 1643, 1718, 2359
BtgI	CCRYGG	2 cut(s)	801, 3230
BtsCI	GGATG	9 cut(s)	1410, 1458, 1801, 1992, 2144, 2203, 2362, 3037, 3169
BtsI	GCAGTG	1 cut(s)	492
BtsIMutI	CAGTG	5 cut(s)	198, 375, 492, 1524, 2574
BveI	ACCTGC	1 cut(s)	2698
Cac8I	GCNNGC	4 cut(s)	928, 946, 1230, 2631
CaiI	CAGNNNCTG	2 cut(s)	53, 2975
CciI	TCATGA	4 cut(s)	250, 1887, 2491, 2806
CfoI	GCGC	4 cut(s)	529, 653, 2776, 2803
Cfr13I	GGNCC	4 cut(s)	751, 887, 1139, 2419
Cfr9I	CCCGGG	1 cut(s)	728
CseI	GACGC	1 cut(s)	1964
Csp6I	GTAC	9 cut(s)	260, 733, 1033, 1166, 1489, 1910, 2279, 2675, 3154
CviQI	GTAC	9 cut(s)	260, 733, 1033, 1166, 1489, 1910, 2279, 2675, 3154
DdeI	CTNAG	7 cut(s)	174, 468, 498, 1075, 1322, 2984, 3021
DraI	TTTAAA	2 cut(s)	2289, 2601
DrdI	GACNNNNNNGTC	1 cut(s)	3251
DseDI	GACNNNNNNGTC	1 cut(s)	3251
EaeI	YGGCCR	3 cut(s)	192, 1542, 1716
Eam1104I	CTCTTC	3 cut(s)	327, 562, 1751
EarI	CTCTTC	3 cut(s)	327, 562, 1751
Eco130I	CCWWGG	2 cut(s)	189, 801
Eco147I	AGGCCT	1 cut(s)	2359
Eco31I	GGTCTC	3 cut(s)	701, 930, 3294
Eco32I	GATATC	2 cut(s)	841, 2401
Eco47I	GGWCC	3 cut(s)	751, 1139, 2419
Eco47III	AGCGCT	1 cut(s)	2775
Eco57I	CTGAAG	5 cut(s)	251, 543, 1164, 2018, 3129
Eco72I	CACGTG	1 cut(s)	485
Eco88I	CYCGRG	2 cut(s)	728, 3078
EcoO109I	RGGNCCY	1 cut(s)	751
EcoRI	GAATTC	1 cut(s)	1393
EcoRII	CCWGG	5 cut(s)	861, 883, 1975, 2206, 2269
EcoRV	GATATC	2 cut(s)	841, 2401
EcoT14I	CCWWGG	2 cut(s)	189, 801
EcoT22I	ATGCAT	1 cut(s)	950
ErhI	CCWWGG	2 cut(s)	189, 801
FalI	AAGNNNNNCTT	4 cut(s)	262, 294, 864, 896
FaqI	GGGAC	5 cut(s)	220, 756, 764, 1437, 2432
FauI	CCCGC	2 cut(s)	1380, 3021
FauNDI	CATATG	3 cut(s)	571, 835, 2319
FbaI	TGATCA	1 cut(s)	2538
FblI	GTMKAC	2 cut(s)	2655, 3132
Fnu4HI	GCNGC	3 cut(s)	1494, 1905, 2057
FokI	GGATG	9 cut(s)	1417, 1465, 1808, 1979, 2151, 2190, 2349, 3044, 3156
Fsp4HI	GCNGC	3 cut(s)	1494, 1905, 2057
FspBI	CTAG	7 cut(s)	299, 962, 1497, 2084, 2144, 3110, 3308
GlaI	GCGC	4 cut(s)	528, 652, 2775, 2802
GluI	GCNGC	3 cut(s)	1494, 1905, 2057
GsaI	CCCAGC	1 cut(s)	2471
GsuI	CTGGAG	2 cut(s)	89, 1959
HaeII	RGCGCY	2 cut(s)	530, 2777
HaeIII	GGCC	6 cut(s)	194, 889, 1544, 1643, 1718, 2359
HapII	CCGG	7 cut(s)	238, 324, 729, 2154, 2306, 3117, 3322
HgaI	GACGC	1 cut(s)	1964
HhaI	GCGC	4 cut(s)	529, 653, 2776, 2803
Hin1I	GRCGYC	1 cut(s)	2496
Hin6I	GCGC	4 cut(s)	527, 651, 2774, 2801
HinP1I	GCGC	4 cut(s)	527, 651, 2774, 2801
HincII	GTYRAC	1 cut(s)	2656
HindII	GTYRAC	1 cut(s)	2656
HindIII	AAGCTT	2 cut(s)	434, 3141
HpaII	CCGG	7 cut(s)	238, 324, 729, 2154, 2306, 3117, 3322
HphI	GGTGA	5 cut(s)	911, 1053, 1355, 2285, 2800
Hpy99I	CGWCG	3 cut(s)	1378, 2657, 3257
HpyAV	CCTTC	8 cut(s)	42, 1011, 1993, 2225, 2836, 3064, 3091, 3279
HpyCH4III	ACNGT	4 cut(s)	18, 287, 1540, 2578
HpyCH4IV	ACGT	3 cut(s)	484, 2281, 2496
HpyF3I	CTNAG	7 cut(s)	174, 468, 498, 1075, 1322, 2984, 3021
HpySE526I	ACGT	3 cut(s)	484, 2281, 2496
Hsp92I	GRCGYC	1 cut(s)	2496
HspAI	GCGC	4 cut(s)	527, 651, 2774, 2801
Kpn2I	TCCGGA	2 cut(s)	3116, 3321
KpnI	GGTACC	1 cut(s)	3157
Ksp22I	TGATCA	1 cut(s)	2538
LguI	GCTCTTC	1 cut(s)	327
LmnI	GCTCC	7 cut(s)	524, 682, 797, 1018, 1225, 2771, 3119
Lsp1109I	GCAGC	3 cut(s)	1480, 1916, 2043
LweI	GCATC	7 cut(s)	50, 160, 913, 2043, 2081, 2890, 3022
MaeI	CTAG	7 cut(s)	299, 962, 1497, 2084, 2144, 3110, 3308
MaeII	ACGT	3 cut(s)	484, 2281, 2496
MaeIII	GTNAC	7 cut(s)	488, 757, 1700, 2195, 2497, 3160, 3209
MfeI	CAATTG	2 cut(s)	1382, 2646
MflI	RGATCY	6 cut(s)	89, 621, 790, 1876, 2593, 2822
MlsI	TGGCCA	2 cut(s)	1544, 1718
MluNI	TGGCCA	2 cut(s)	1544, 1718
MlyI	GAGTC	2 cut(s)	548, 1113
MmeI	TCCRAC	5 cut(s)	724, 1198, 1751, 1773, 2473
Mox20I	TGGCCA	2 cut(s)	1544, 1718
Mph1103I	ATGCAT	1 cut(s)	950
MroI	TCCGGA	2 cut(s)	3116, 3321
MroXI	GAANNNNTTC	3 cut(s)	723, 1563, 2430
MscI	TGGCCA	2 cut(s)	1544, 1718
MseI	TTAA	8 cut(s)	183, 398, 849, 980, 1646, 1751, 2288, 2600
MslI	CAYNNNNRTG	5 cut(s)	1134, 2271, 2367, 2700, 3053
Msp20I	TGGCCA	2 cut(s)	1544, 1718
MspA1I	CMGCKG	3 cut(s)	1433, 1907, 2681
MspI	CCGG	7 cut(s)	238, 324, 729, 2154, 2306, 3117, 3322
MspR9I	CCNGG	8 cut(s)	238, 729, 730, 863, 885, 1977, 2208, 2271
MunI	CAATTG	2 cut(s)	1382, 2646
Mva1269I	GAATGC	2 cut(s)	1367, 1927
MvaI	CCWGG	5 cut(s)	863, 885, 1977, 2208, 2271
NciI	CCSGG	3 cut(s)	238, 729, 730
NcoI	CCATGG	1 cut(s)	801
NdeI	CATATG	3 cut(s)	571, 835, 2319
NlaIV	GGNNCC	6 cut(s)	623, 752, 799, 1872, 2420, 3155
NmuCI	GTSAC	5 cut(s)	488, 757, 2497, 3160, 3209
NsiI	ATGCAT	1 cut(s)	950
NspI	RCATGY	4 cut(s)	948, 1809, 2016, 3301
OliI	CACNNNNGTG	1 cut(s)	2271
PaeI	GCATGC	1 cut(s)	948
PagI	TCATGA	4 cut(s)	250, 1887, 2491, 2806
PceI	AGGCCT	1 cut(s)	2359
PciI	ACATGT	2 cut(s)	2012, 3297
PciSI	GCTCTTC	1 cut(s)	327
PctI	GAATGC	2 cut(s)	1367, 1927
PdmI	GAANNNNTTC	3 cut(s)	723, 1563, 2430
PflFI	GACNNNGTC	2 cut(s)	755, 1574
PflMI	CCANNNNNTGG	5 cut(s)	713, 896, 2323, 2455, 2669
PfoI	TCCNGGA	3 cut(s)	236, 1975, 2206
PkrI	GCNGC	3 cut(s)	1495, 1906, 2058
PleI	GAGTC	2 cut(s)	548, 1112
PmaCI	CACGTG	1 cut(s)	485
PmlI	CACGTG	1 cut(s)	485
PpsI	GAGTC	2 cut(s)	548, 1112
Ppu21I	YACGTR	2 cut(s)	485, 2282
PpuMI	RGGWCCY	1 cut(s)	751
PscI	ACATGT	2 cut(s)	2012, 3297
PshBI	ATTAAT	2 cut(s)	183, 398
PsiI	TTATAA	1 cut(s)	2022
Psp5II	RGGWCCY	1 cut(s)	751
Psp6I	CCWGG	5 cut(s)	861, 883, 1975, 2206, 2269
PspCI	CACGTG	1 cut(s)	485
PspFI	CCCAGC	1 cut(s)	2467
PspGI	CCWGG	5 cut(s)	861, 883, 1975, 2206, 2269
PspN4I	GGNNCC	6 cut(s)	623, 752, 799, 1872, 2420, 3155
PspPI	GGNCC	4 cut(s)	751, 887, 1139, 2419
PspPPI	RGGWCCY	1 cut(s)	751
PstI	CTGCAG	1 cut(s)	49
PstNI	CAGNNNCTG	2 cut(s)	53, 2975
PsuI	RGATCY	6 cut(s)	89, 621, 790, 1876, 2593, 2822
PsyI	GACNNNGTC	2 cut(s)	755, 1574
PvuII	CAGCTG	3 cut(s)	1433, 1907, 2681
RsaI	GTAC	9 cut(s)	261, 734, 1034, 1167, 1490, 1911, 2280, 2676, 3155
RsaNI	GTAC	9 cut(s)	260, 733, 1033, 1166, 1489, 1910, 2279, 2675, 3154
RseI	CAYNNNNRTG	5 cut(s)	1134, 2271, 2367, 2700, 3053
SalI	GTCGAC	1 cut(s)	2654
SapI	GCTCTTC	1 cut(s)	327
SaqAI	TTAA	8 cut(s)	183, 398, 849, 980, 1646, 1751, 2288, 2600
SatI	GCNGC	3 cut(s)	1494, 1905, 2057
Sau96I	GGNCC	4 cut(s)	751, 887, 1139, 2419
SchI	GAGTC	2 cut(s)	548, 1113
ScrFI	CCNGG	8 cut(s)	238, 729, 730, 863, 885, 1977, 2208, 2271
SfaNI	GCATC	7 cut(s)	50, 160, 913, 2043, 2081, 2890, 3022
SfcI	CTRYAG	1 cut(s)	45
SinI	GGWCC	3 cut(s)	751, 1139, 2419
SmaI	CCCGGG	1 cut(s)	730
SmiMI	CAYNNNNRTG	5 cut(s)	1134, 2271, 2367, 2700, 3053
SmlI	CTYRAG	2 cut(s)	1013, 1865
SmoI	CTYRAG	2 cut(s)	1013, 1865
SphI	GCATGC	1 cut(s)	948
SseBI	AGGCCT	1 cut(s)	2359
SsiI	CCGC	6 cut(s)	162, 276, 1036, 1387, 1837, 3028
SspI	AATATT	1 cut(s)	2522
SspMI	CTAG	7 cut(s)	299, 962, 1497, 2084, 2144, 3110, 3308
StuI	AGGCCT	1 cut(s)	2359
StyD4I	CCNGG	8 cut(s)	236, 727, 728, 861, 883, 1975, 2206, 2269
StyI	CCWWGG	2 cut(s)	189, 801
TaaI	ACNGT	4 cut(s)	18, 287, 1540, 2578
TaiI	ACGT	3 cut(s)	487, 2284, 2499
TaqI	TCGA	5 cut(s)	540, 1376, 1690, 2391, 2655
TatI	WGTACW	2 cut(s)	1165, 1909
Tru1I	TTAA	8 cut(s)	183, 398, 849, 980, 1646, 1751, 2288, 2600
Tru9I	TTAA	8 cut(s)	183, 398, 849, 980, 1646, 1751, 2288, 2600
TscAI	CASTG	5 cut(s)	205, 375, 499, 1531, 2581
TseFI	GTSAC	5 cut(s)	488, 757, 2497, 3160, 3209
TseI	GCWGC	3 cut(s)	1493, 1904, 2056
Tsp45I	GTSAC	5 cut(s)	488, 757, 2497, 3160, 3209
TspGWI	ACGGA	2 cut(s)	776, 3219
TspMI	CCCGGG	1 cut(s)	728
TspRI	CASTG	5 cut(s)	205, 375, 499, 1531, 2581
Tth111I	GACNNNGTC	2 cut(s)	755, 1574
Van91I	CCANNNNNTGG	5 cut(s)	713, 896, 2323, 2455, 2669
VpaK11BI	GGWCC	3 cut(s)	751, 1139, 2419
VspI	ATTAAT	2 cut(s)	183, 398
XapI	RAATTY	5 cut(s)	1236, 1295, 1358, 1393, 2711
XbaI	TCTAGA	5 cut(s)	298, 961, 2143, 3109, 3307
XceI	RCATGY	4 cut(s)	948, 1809, 2016, 3301
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3323
XmaI	CCCGGG	1 cut(s)	728
XmiI	GTMKAC	2 cut(s)	2655, 3132
XmnI	GAANNNNTTC	3 cut(s)	723, 1563, 2430
XspI	CTAG	7 cut(s)	299, 962, 1497, 2084, 2144, 3110, 3308
ZraI	GACGTC	1 cut(s)	2497
Zsp2I	ATGCAT	1 cut(s)	950

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.