Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Forward (+)

2829280 .. 2839868

Gene Structure 10589 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1041300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3339 bp

ATGAGTAGGACGATAAGGTTGTATGGATTCCCTTCGGGTGTGACTGCAGAAGCAGTTGTTCGATTGCTGGAGAACGAAACAGGAAGCGGATCTGTCTATGCTGTGAGGTTAAGAAAAGCCAAAGATGGGAGAAGATATTATGCTATTGTTCAGTTTGACACAACCAGAAATGCTGAATTTATTCACGACATGGCCAATGCTAAAAACTTGTACTATGGCGCTTCCTATCTGCAATCTTGGGAGGTGGCTCATGATATTGTACCAAATCCAAGAGTCTCTTTGCATACCTTTGAAAACATAAAGCTGGATTTTGGCTGCAAGATCGCAACAAATAAGCTATTTAGTTTCTGGAATATGAACAATGTTTCAGTGGATTTTGGGATTGGATTGCGGAAACTCGTTTGTCGTCTGAAATATGACGCTCTGGAGTATATGCTCGAACTTTCCTATGAAAACATCTGGCAGATTGAGTTGCATCGTACACGCGGTCGTGCTGCTAAGCATCTTCTGATTCAGATGCACGGAGCACCTCGGATTTCTAAGAAAGTCATACGCTCTTCAGGACAAGCATATGAAGATCGTATTCTGAACTATTTCATGGACTGTGATGACCGGTGGGTCCGGACAACTGATTTCACTCCATCTCGTGCTATTGGGCAGTCTTTTGCATTAAGCTTGGAGCTTCCTCGTAGTCTCAAACTTCCAGATTTTCGAGAAAACTTTCCTGGGTATGAAGAGATTGAAGGTAACCTTGTCTTGGAGATTGGTCCCCCTTATTCTTGCAACCCGAGTCTGGGTCCTATGGTTTTTCCTCCTCGAGGGGTTAATGTGCCATATGATATCTTGTTTAAAGTACATTCTCTGCTTCAGCTTGGGTGTCTTTCAGGGCCAACACTCGATGTTAAATTTTATAAGATGGTTGATCCCCTCAGAATTGATCGTGCATGTATAGAGTATGCCCTAGATAAATTATATAATTTGAAAGAGACCTGCTATAAACCTTCAGAGTGGCTTTATGAGCAGTACATGAAGTACCTCACGTCAAAGAGACGTCCACAGTCATCTGTCATATCCTTAGATTCTGGGTTGGTATATGTACGCAGGGTCCAAATTACACCATGTCGTGTATTTTCTTCTGGTCCTGAGGTCAATGTCTCAAATCGTGTACTACGCAATTATCCAGATGATATTGATAACTTCATTCGTGTTTCCTTTGTTGACGAGGAGATGGATCGAGTTTATTCTGCGGATTTGCTTCCACGTACTTCTGCTGCCTATGAGGATGGGAAAACTGACATCTACAAGCGGATTCTTTTTATTCTCAGAAACGGCATAGTAATTGGTGCAAAGAAATTCGAGTTTCTCGCCTTCTCATCTAGTCAATTGCGAGAGAATTCTATGTGGATGTTTGCTTCAAGACAGGGACTTACTGCAGCTGATATAAGAGAGTGGATGGGGGATTTTCGTCGCATAAAAAATGTGGCAAAATATGCTGCCAGATTGGGTCAATCCTTTGGTTCGTCCACTGAAACTTTAAATGTTAGAAAACATGAAATGGAAGTTATTCCCGATGTAAAAAACAATAAATACGTCTTCTCCGATGGGATTGGGAAAATATCTGCCAAGTTTGCTAAGAAGGTGGCCTCAAAATGTGGCTTTAATGGGACTCCATCTGCCTTTCAGATTCGATATGGTGGGTACAAAGGTGTCGTGGCCGTTGATCCAACTTCATCAATGAAATTATCATTGAGGAGGAGCATGTCCAAGTATGAATCAGAAAACACAAAGTTAGATGTTTTAGCATGTAGCAAGATTCAACCTTGTTTTCTGAACCGCCAGTTGATAACTCTTTTATCGACCCTTGGGGTTCCAGATCATGTTTTCAAGAAAAAACAAAGGGCAGCTGTACAACAATTGAATGCTATATTGACTGACCCACTAAAAGCACGAGAGGCTCTGGATTTGATGTCTCCAGGGGAGACTACCAACTGCTTGAAGGAATTGCTTATATGTGGTTATAAACCTGATGTTGAACCATTCATTTCAATGATGCTGCAAACATTTCGGGCATCAAAGTTGCTAGAGTTGCGGACGAAAACAAGGATCTTTATTCCAGATGGACGAGCAATGATGGGATGTCTAGATGAAACCAGAACATTGGAACATGGTGAGGTGTTTGTGCAGTTCTCTGGTAACAGGCATACTGTAAGTGCATCAAACCAGCAATTCATTATTGTGGGGAAGGTAGTTGTTGCGAAGAACCCTTGTTTGCATCCTGGCGATGTACGTGTTTTAAAGGCTGTGGATGTGCCAGGGTTGTACCATATGGTTGATTGTGTCGTTTTTCCACAAAAAGGACCTAGGCCTCATCCAAATGAATGTTCAGGAAGTGATCTTGATGGAGATATCTACTATGTTTGTTGGGACAAAGAACTAATTCCTCGGAAGCAAATCAAACCCATGGATTATACCCCAGCACCAACTATAGAATTGGATCATGATGTCACAATTGAGGAAGTTGAAGAATATTTTGTGAACTACATGGTCAATGATAGCTTGGGCATAATTGCCAATGCCCACACTGTGTTTGCAGACAAAGATCCCTTAAAAGCAATGAGCAAAGAATGTATAGAGCTTGCAAAGCTATTTTCAATTGCCGTTGACTTTCCAAAAACTGGTGTGCCGGCTGTAATACCTCCACAGTTATACGCCAAGGAATATCCAGATTTCATGGAAAAACTTGACAAACCCACCTATCAGTCATCCAATGTAATTGGAGCTCTTTTTCGTGAGGTGAAGGACATTGCGCCTCATGAAGGCTCTATCACATCCTTCACTCGTCGAGTGGCGAAGCAGTCATACGACCCTGACATGGAAGTTGATGGCTTCGAGGATTACATTGAAGATGCTATCTATTACAAGGGCAATTATGATTACAAGTTGGGAAATCTGATGGAACATTATGGGATCAAGACAGAGGCTGAAATACTTAGTGGAAGTGTTATGAGAATGTCAAAATCTTTCACTAAGAGGCGGGATGCAGATTCTATAAGGGTGGCTGTGAGATCGTTGAGGAAGGAAGCTAGGGCGTGGTTCAATGAGAAGGGAACTGGTTTAGATTCCGGAGCTGATGATGTTTATGCCAAAGCTTCAGCTTGGTACCATGTCACATATCATCCTGATTATTTTGGTACATACAACGAGGGGATGAATCGTGACCATTTCATCAGCTTTCCATGGTGCGTTTATGACAAGCTCGTCCTCATCAAAAAGGATAAAGCAAGTATACACATGTCATCTCTAGGACGCCAATTCTGGGATGGGTTGCATTTGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

1113

Amino Acids

126.71

Weight (kDa)

8.62

Isoelectric Point (pI)

39.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_RdRP1_2	PF26250	4 - 85	4.8e-16	RdRP1/2, RRM domain
PH_RDR2	PF24823	102 - 251	7.1e-40	RDR2 PH-like domain
RDR6_2nd	PF24577	143 - 227	4.3e-07	RNA-dependent RNA polymerase 6 second domain
RdRP_helical	PF26252	267 - 351	1.3e-10	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	371 - 932	6e-197	RNA dependent RNA polymerase
RdRP_head	PF26253	953 - 1092	6.2e-47	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	912, 2019
AasI	GACNNNNNNGTC	2 cut(s)	617, 3257
AatII	GACGTC	1 cut(s)	1054
AbsI	CCTCGAGG	1 cut(s)	816
Acc36I	ACCTGC	1 cut(s)	998
Acc65I	GGTACC	1 cut(s)	3159
AccB1I	GGYRCC	1 cut(s)	3159
AccB7I	CCANNNNNTGG	1 cut(s)	2675
AccI	GTMKAC	1 cut(s)	3286
AccII	CGCG	1 cut(s)	486
AccIII	TCCGGA	2 cut(s)	621, 3122
AciI	CCGC	8 cut(s)	87, 391, 486, 1247, 1306, 1834, 2089, 3034
AclWI	GGATC	8 cut(s)	97, 917, 1239, 1715, 2111, 2502, 2594, 2975
AcoI	YGGCCR	2 cut(s)	192, 1713
AcsI	RAATTY	4 cut(s)	176, 905, 1352, 1393
AcuI	CTGAAG	4 cut(s)	543, 851, 987, 3135
AcyI	GRCGYC	2 cut(s)	1051, 3307
AdeI	CACNNNGTG	1 cut(s)	2584
AflIII	ACRYGT	2 cut(s)	2287, 3291
AgeI	ACCGGT	1 cut(s)	612
AjiI	CACGTC	1 cut(s)	1041
AjnI	CCWGG	4 cut(s)	724, 1972, 2275, 2311
AjuI	GAANNNNNNNTTGG	2 cut(s)	2365, 2397
Alw21I	GWGCWC	2 cut(s)	529, 2782
Alw26I	GTCTC	7 cut(s)	280, 698, 980, 1042, 1159, 1973, 1974
AlwI	GGATC	8 cut(s)	97, 917, 1239, 1715, 2111, 2502, 2594, 2975
AlwNI	CAGNNNCTG	1 cut(s)	2981
Ama87I	CYCGRG	2 cut(s)	787, 816
Aor13HI	TCCGGA	2 cut(s)	621, 3122
AoxI	GGCC	5 cut(s)	192, 887, 1641, 1713, 2363
ApeKI	GCWGC	7 cut(s)	315, 494, 1271, 1433, 1493, 1901, 2053
ApoI	RAATTY	4 cut(s)	176, 905, 1352, 1393
AsiGI	ACCGGT	1 cut(s)	612
Asp700I	GAANNNNTTC	5 cut(s)	180, 593, 720, 1563, 2436
Asp718I	GGTACC	1 cut(s)	3159
AspA2I	CCTAGG	1 cut(s)	2360
AspLEI	GCGC	2 cut(s)	221, 2809
AspS9I	GGNCC	7 cut(s)	619, 767, 797, 887, 1105, 1139, 2357
AsuHPI	GGTGA	2 cut(s)	2180, 2806
AvaI	CYCGRG	2 cut(s)	787, 816
AvaII	GGWCC	6 cut(s)	619, 767, 797, 1105, 1139, 2357
AvrII	CCTAGG	1 cut(s)	2360
AxyI	CCTNAGG	1 cut(s)	1143
BaeI	ACNNNNGTAYC	2 cut(s)	1690, 1723
BalI	TGGCCA	1 cut(s)	194
BanI	GGYRCC	1 cut(s)	3159
BanII	GRGCYC	1 cut(s)	2782
BarI	GAAGNNNNNNTAC	2 cut(s)	2843, 2875
BauI	CACGAG	2 cut(s)	645, 1947
BbsI	GAAGAC	1 cut(s)	1585
Bbv12I	GWGCWC	2 cut(s)	529, 2782
BbvI	GCAGC	7 cut(s)	302, 481, 1258, 1445, 1480, 1913, 2040
BceAI	ACGGC	3 cut(s)	1345, 1700, 2642
BcgI	CGANNNNNNTGC	2 cut(s)	2045, 2079
BciT130I	CCWGG	4 cut(s)	726, 1974, 2277, 2313
BcoDI	GTCTC	7 cut(s)	280, 698, 980, 1042, 1159, 1973, 1974
BfaI	CTAG	7 cut(s)	962, 1377, 2081, 2141, 2361, 3084, 3302
BfmI	CTRYAG	3 cut(s)	45, 1431, 2484
BfoI	RGCGCY	1 cut(s)	222
BfuAI	ACCTGC	1 cut(s)	998
BisI	GCNGC	7 cut(s)	316, 495, 1272, 1434, 1494, 1902, 2054
BlnI	CCTAGG	1 cut(s)	2360
BlpI	GCTNAGC	1 cut(s)	498
BlsI	GCNGC	7 cut(s)	317, 496, 1273, 1435, 1495, 1903, 2055
Bme1390I	CCNGG	4 cut(s)	726, 1974, 2277, 2313
Bme18I	GGWCC	6 cut(s)	619, 767, 797, 1105, 1139, 2357
BmeT110I	CYCGRG	2 cut(s)	787, 816
BmgBI	CACGTC	1 cut(s)	1041
BmgT120I	GGNCC	7 cut(s)	619, 767, 797, 887, 1105, 1139, 2357
BmiI	GGNNCC	6 cut(s)	620, 769, 798, 1106, 1869, 3161
BmrFI	CCNGG	4 cut(s)	726, 1974, 2277, 2313
BmsI	GCATC	9 cut(s)	484, 507, 511, 2040, 2078, 2222, 2281, 2896, 3028
BpiI	GAAGAC	1 cut(s)	1585
BpmI	CTGGAG	3 cut(s)	89, 446, 1956
Bpu1102I	GCTNAGC	1 cut(s)	498
BsaAI	YACGTR	2 cut(s)	1262, 2288
BsaHI	GRCGYC	2 cut(s)	1051, 3307
BsaI	GGTCTC	1 cut(s)	980
BsaWI	WCCGGW	3 cut(s)	612, 621, 3122
BsaXI	ACNNNNNCTCC	2 cut(s)	1580, 1610
Bse118I	RCCGGY	2 cut(s)	612, 2683
Bse1I	ACTGG	3 cut(s)	1837, 2680, 3115
Bse21I	CCTNAGG	1 cut(s)	1143
Bse3DI	GCAATG	3 cut(s)	2133, 2619, 2802
BseAI	TCCGGA	2 cut(s)	621, 3122
BseBI	CCWGG	4 cut(s)	726, 1974, 2277, 2313
BseMI	GCAATG	3 cut(s)	2133, 2619, 2802
BseMII	CTCAG	3 cut(s)	943, 1134, 1336
BseNI	ACTGG	3 cut(s)	1837, 2680, 3115
BseRI	GAGGAG	4 cut(s)	804, 1238, 1765, 1768
BseXI	GCAGC	7 cut(s)	302, 481, 1258, 1445, 1480, 1913, 2040
BseYI	CCCAGC	1 cut(s)	2473
BsgI	GTGCAG	1 cut(s)	2201
Bsh1236I	CGCG	1 cut(s)	486
Bsh1285I	CGRYCG	1 cut(s)	490
BshFI	GGCC	5 cut(s)	194, 889, 1643, 1715, 2365
BshNI	GGYRCC	1 cut(s)	3159
BshTI	ACCGGT	1 cut(s)	612
BsiEI	CGRYCG	1 cut(s)	490
BsiHKAI	GWGCWC	2 cut(s)	529, 2782
BsiHKCI	CYCGRG	2 cut(s)	787, 816
BsiSI	CCGG	4 cut(s)	613, 622, 2684, 3123
BslFI	GGGAC	4 cut(s)	753, 1437, 1678, 2438
BsmAI	GTCTC	7 cut(s)	280, 698, 980, 1042, 1159, 1973, 1974
BsmBI	CGTCTC	1 cut(s)	1042
BsmFI	GGGAC	4 cut(s)	753, 1437, 1678, 2438
BsmI	GAATGC	1 cut(s)	1924
BsnI	GGCC	5 cut(s)	194, 889, 1643, 1715, 2365
Bso31I	GGTCTC	1 cut(s)	980
BsoBI	CYCGRG	2 cut(s)	787, 816
Bsp1286I	GDGCHC	2 cut(s)	529, 2782
Bsp13I	TCCGGA	2 cut(s)	621, 3122
Bsp1407I	TGTACA	1 cut(s)	1906
Bsp1720I	GCTNAGC	1 cut(s)	498
Bsp19I	CCATGG	2 cut(s)	2460, 3236
BspACI	CCGC	8 cut(s)	87, 391, 486, 1247, 1306, 1834, 2089, 3034
BspANI	GGCC	5 cut(s)	194, 889, 1643, 1715, 2365
BspCNI	CTCAG	3 cut(s)	942, 1135, 1335
BspEI	TCCGGA	2 cut(s)	621, 3122
BspFNI	CGCG	1 cut(s)	486
BspHI	TCATGA	3 cut(s)	250, 2497, 2812
BspLI	GGNNCC	6 cut(s)	620, 769, 798, 1106, 1869, 3161
BspMAI	CTGCAG	2 cut(s)	49, 1435
BspMI	ACCTGC	1 cut(s)	998
BspPI	GGATC	8 cut(s)	97, 917, 1239, 1715, 2111, 2502, 2594, 2975
BspQI	GCTCTTC	1 cut(s)	562
BspT107I	GGYRCC	1 cut(s)	3159
BspTNI	GGTCTC	1 cut(s)	980
BsrDI	GCAATG	3 cut(s)	2133, 2619, 2802
BsrFI	RCCGGY	2 cut(s)	612, 2683
BsrGI	TGTACA	1 cut(s)	1906
BsrI	ACTGG	3 cut(s)	1837, 2680, 3115
BssAI	RCCGGY	2 cut(s)	612, 2683
BssNAI	GTATAC	1 cut(s)	3287
BssNI	GRCGYC	2 cut(s)	1051, 3307
BssSI	CACGAG	2 cut(s)	645, 1947
BssT1I	CCWWGG	5 cut(s)	1861, 2360, 2460, 2712, 3236
Bst1107I	GTATAC	1 cut(s)	3287
Bst2BI	CACGAG	2 cut(s)	645, 1947
Bst2UI	CCWGG	4 cut(s)	726, 1974, 2277, 2313
Bst4CI	ACNGT	5 cut(s)	605, 1059, 2206, 2584, 2703
Bst6I	CTCTTC	2 cut(s)	562, 729
BstACI	GRCGYC	2 cut(s)	1051, 3307
BstAPI	GCANNNNNTGC	1 cut(s)	1490
BstAUI	TGTACA	1 cut(s)	1906
BstBAI	YACGTR	2 cut(s)	1262, 2288
BstC8I	GCNNGC	2 cut(s)	2637, 2685
BstDEI	CTNAG	9 cut(s)	498, 540, 929, 1075, 1143, 1322, 1632, 2990, 3027
BstDSI	CCRYGG	2 cut(s)	2460, 3236
BstEII	GGTNACC	1 cut(s)	746
BstENI	CCTNNNNNAGG	2 cut(s)	816, 2814
BstFNI	CGCG	1 cut(s)	486
BstH2I	RGCGCY	1 cut(s)	222
BstHHI	GCGC	2 cut(s)	221, 2809
BstMAI	GTCTC	7 cut(s)	280, 698, 980, 1042, 1159, 1973, 1974
BstMCI	CGRYCG	1 cut(s)	490
BstMWI	GCNNNNNNNGC	6 cut(s)	1018, 1490, 1628, 1952, 2086, 2641
BstNI	CCWGG	4 cut(s)	726, 1974, 2277, 2313
BstNSI	RCATGY	4 cut(s)	948, 1762, 1806, 3295
BstPI	GGTNACC	1 cut(s)	746
BstSCI	CCNGG	4 cut(s)	724, 1972, 2275, 2311
BstSFI	CTRYAG	3 cut(s)	45, 1431, 2484
BstUI	CGCG	1 cut(s)	486
BstV1I	GCAGC	7 cut(s)	302, 481, 1258, 1445, 1480, 1913, 2040
BstV2I	GAAGAC	1 cut(s)	1585
BstX2I	RGATCY	3 cut(s)	89, 2103, 2599
BstXI	CCANNNNNNTGG	1 cut(s)	2158
BstYI	RGATCY	3 cut(s)	89, 2103, 2599
BstZ17I	GTATAC	1 cut(s)	3287
Bsu36I	CCTNAGG	1 cut(s)	1143
BsuRI	GGCC	5 cut(s)	194, 889, 1643, 1715, 2365
BtgI	CCRYGG	2 cut(s)	2460, 3236
BtgZI	GCGATG	1 cut(s)	2295
BtrI	CACGTC	1 cut(s)	1041
BtsIMutI	CAGTG	3 cut(s)	375, 1524, 2580
BveI	ACCTGC	1 cut(s)	998
Cac8I	GCNNGC	2 cut(s)	2637, 2685
CaiI	CAGNNNCTG	1 cut(s)	2981
CciI	TCATGA	3 cut(s)	250, 2497, 2812
CfoI	GCGC	2 cut(s)	221, 2809
Cfr10I	RCCGGY	2 cut(s)	612, 2683
Cfr13I	GGNCC	7 cut(s)	619, 767, 797, 887, 1105, 1139, 2357
CseI	GACGC	2 cut(s)	428, 3315
CspAI	ACCGGT	1 cut(s)	612
DdeI	CTNAG	9 cut(s)	498, 540, 929, 1075, 1143, 1322, 1632, 2990, 3027
DraI	TTTAAA	3 cut(s)	850, 1536, 2295
DraIII	CACNNNGTG	1 cut(s)	2584
DrdI	GACNNNNNNGTC	2 cut(s)	617, 3257
DseDI	GACNNNNNNGTC	2 cut(s)	617, 3257
EaeI	YGGCCR	2 cut(s)	192, 1713
Eam1104I	CTCTTC	2 cut(s)	562, 729
EarI	CTCTTC	2 cut(s)	562, 729
Ecl136II	GAGCTC	1 cut(s)	2780
Eco130I	CCWWGG	5 cut(s)	1861, 2360, 2460, 2712, 3236
Eco147I	AGGCCT	1 cut(s)	2365
Eco24I	GRGCYC	1 cut(s)	2782
Eco31I	GGTCTC	1 cut(s)	980
Eco32I	GATATC	2 cut(s)	841, 2407
Eco47I	GGWCC	6 cut(s)	619, 767, 797, 1105, 1139, 2357
Eco53kI	GAGCTC	1 cut(s)	2780
Eco57I	CTGAAG	4 cut(s)	543, 851, 987, 3135
Eco81I	CCTNAGG	1 cut(s)	1143
Eco88I	CYCGRG	2 cut(s)	787, 816
Eco91I	GGTNACC	1 cut(s)	746
EcoICRI	GAGCTC	1 cut(s)	2780
EcoNI	CCTNNNNNAGG	2 cut(s)	816, 2814
EcoO109I	RGGNCCY	2 cut(s)	797, 2357
EcoO65I	GGTNACC	1 cut(s)	746
EcoRI	GAATTC	1 cut(s)	1393
EcoRII	CCWGG	4 cut(s)	724, 1972, 2275, 2311
EcoRV	GATATC	2 cut(s)	841, 2407
EcoT14I	CCWWGG	5 cut(s)	1861, 2360, 2460, 2712, 3236
EcoT38I	GRGCYC	1 cut(s)	2782
ErhI	CCWWGG	5 cut(s)	1861, 2360, 2460, 2712, 3236
Esp3I	CGTCTC	1 cut(s)	1042
FalI	AAGNNNNNCTT	4 cut(s)	262, 294, 735, 767
FaqI	GGGAC	4 cut(s)	753, 1437, 1678, 2438
FauI	CCCGC	1 cut(s)	3027
FauNDI	CATATG	3 cut(s)	571, 835, 2325
FblI	GTMKAC	1 cut(s)	3286
Fnu4HI	GCNGC	7 cut(s)	316, 495, 1272, 1434, 1494, 1902, 2054
FriOI	GRGCYC	1 cut(s)	2782
Fsp4HI	GCNGC	7 cut(s)	316, 495, 1272, 1434, 1494, 1902, 2054
FspBI	CTAG	7 cut(s)	962, 1377, 2081, 2141, 2361, 3084, 3302
GlaI	GCGC	2 cut(s)	220, 2808
GluI	GCNGC	7 cut(s)	316, 495, 1272, 1434, 1494, 1902, 2054
GsaI	CCCAGC	1 cut(s)	2477
GsuI	CTGGAG	3 cut(s)	89, 446, 1956
HaeII	RGCGCY	1 cut(s)	222
HaeIII	GGCC	5 cut(s)	194, 889, 1643, 1715, 2365
HapII	CCGG	4 cut(s)	613, 622, 2684, 3123
HgaI	GACGC	2 cut(s)	428, 3315
HhaI	GCGC	2 cut(s)	221, 2809
Hin1I	GRCGYC	2 cut(s)	1051, 3307
Hin6I	GCGC	2 cut(s)	219, 2807
HinP1I	GCGC	2 cut(s)	219, 2807
HincII	GTYRAC	2 cut(s)	1219, 2662
HindII	GTYRAC	2 cut(s)	1219, 2662
HindIII	AAGCTT	2 cut(s)	673, 3147
HpaII	CCGG	4 cut(s)	613, 622, 2684, 3123
HphI	GGTGA	2 cut(s)	2180, 2806
Hpy166II	GTNNAC	8 cut(s)	482, 1055, 1166, 1219, 1524, 2536, 2662, 3287
Hpy8I	GTNNAC	8 cut(s)	482, 1055, 1166, 1219, 1524, 2536, 2662, 3287
Hpy99I	CGWCG	2 cut(s)	1470, 2844
HpyCH4III	ACNGT	5 cut(s)	605, 1059, 2206, 2584, 2703
HpyCH4IV	ACGT	5 cut(s)	1040, 1051, 1261, 1590, 2287
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1018, 1490, 1628, 1952, 2086, 2641
HpyF3I	CTNAG	9 cut(s)	498, 540, 929, 1075, 1143, 1322, 1632, 2990, 3027
HpySE526I	ACGT	5 cut(s)	1040, 1051, 1261, 1590, 2287
Hsp92I	GRCGYC	2 cut(s)	1051, 3307
HspAI	GCGC	2 cut(s)	219, 2807
Kpn2I	TCCGGA	2 cut(s)	621, 3122
KpnI	GGTACC	1 cut(s)	3163
KroI	GCCGGC	1 cut(s)	2683
KroNI	GCCGGC	1 cut(s)	2685
LguI	GCTCTTC	1 cut(s)	562
LmnI	GCTCC	5 cut(s)	524, 679, 1755, 2777, 3125
Lsp1109I	GCAGC	7 cut(s)	302, 481, 1258, 1445, 1480, 1913, 2040
LweI	GCATC	9 cut(s)	484, 507, 511, 2040, 2078, 2222, 2281, 2896, 3028
MaeI	CTAG	7 cut(s)	962, 1377, 2081, 2141, 2361, 3084, 3302
MaeII	ACGT	5 cut(s)	1040, 1051, 1261, 1590, 2287
MaeIII	GTNAC	6 cut(s)	40, 746, 2192, 2503, 3166, 3215
MfeI	CAATTG	4 cut(s)	1382, 1913, 2508, 2652
MflI	RGATCY	3 cut(s)	89, 2103, 2599
MhlI	GDGCHC	2 cut(s)	529, 2782
MlsI	TGGCCA	1 cut(s)	194
MluNI	TGGCCA	1 cut(s)	194
MlyI	GAGTC	3 cut(s)	282, 799, 1660
MmeI	TCCRAC	1 cut(s)	1748
Mox20I	TGGCCA	1 cut(s)	194
MroI	TCCGGA	2 cut(s)	621, 3122
MroNI	GCCGGC	1 cut(s)	2683
MroXI	GAANNNNTTC	5 cut(s)	180, 593, 720, 1563, 2436
MscI	TGGCCA	1 cut(s)	194
MseI	TTAA	9 cut(s)	110, 671, 825, 849, 903, 1535, 1659, 2294, 2606
MslI	CAYNNNNRTG	3 cut(s)	2045, 2234, 2373
Msp20I	TGGCCA	1 cut(s)	194
MspA1I	CMGCKG	2 cut(s)	1436, 1904
MspI	CCGG	4 cut(s)	613, 622, 2684, 3123
MspR9I	CCNGG	4 cut(s)	726, 1974, 2277, 2313
MunI	CAATTG	4 cut(s)	1382, 1913, 2508, 2652
Mva1269I	GAATGC	1 cut(s)	1924
MvaI	CCWGG	4 cut(s)	726, 1974, 2277, 2313
MvnI	CGCG	1 cut(s)	486
MwoI	GCNNNNNNNGC	6 cut(s)	1018, 1490, 1628, 1952, 2086, 2641
NaeI	GCCGGC	1 cut(s)	2685
NcoI	CCATGG	2 cut(s)	2460, 3236
NdeI	CATATG	3 cut(s)	571, 835, 2325
NgoMIV	GCCGGC	1 cut(s)	2683
NlaIV	GGNNCC	6 cut(s)	620, 769, 798, 1106, 1869, 3161
NmuCI	GTSAC	4 cut(s)	40, 2503, 3166, 3215
NspI	RCATGY	4 cut(s)	948, 1762, 1806, 3295
PaeR7I	CTCGAG	1 cut(s)	816
PagI	TCATGA	3 cut(s)	250, 2497, 2812
PceI	AGGCCT	1 cut(s)	2365
PciI	ACATGT	1 cut(s)	3291
PciSI	GCTCTTC	1 cut(s)	562
PcsI	WCGNNNNNNNCGW	1 cut(s)	1596
PctI	GAATGC	1 cut(s)	1924
PdiI	GCCGGC	1 cut(s)	2685
PdmI	GAANNNNTTC	5 cut(s)	180, 593, 720, 1563, 2436
PflMI	CCANNNNNTGG	1 cut(s)	2675
PinAI	ACCGGT	1 cut(s)	612
PkrI	GCNGC	7 cut(s)	317, 496, 1273, 1435, 1495, 1903, 2055
PleI	GAGTC	3 cut(s)	281, 798, 1660
PpsI	GAGTC	3 cut(s)	281, 798, 1660
Ppu21I	YACGTR	2 cut(s)	1262, 2288
PpuMI	RGGWCCY	2 cut(s)	797, 2357
PscI	ACATGT	1 cut(s)	3291
PsiI	TTATAA	2 cut(s)	912, 2019
Psp124BI	GAGCTC	1 cut(s)	2782
Psp5II	RGGWCCY	2 cut(s)	797, 2357
Psp6I	CCWGG	4 cut(s)	724, 1972, 2275, 2311
PspEI	GGTNACC	1 cut(s)	746
PspFI	CCCAGC	1 cut(s)	2473
PspGI	CCWGG	4 cut(s)	724, 1972, 2275, 2311
PspN4I	GGNNCC	6 cut(s)	620, 769, 798, 1106, 1869, 3161
PspPI	GGNCC	7 cut(s)	619, 767, 797, 887, 1105, 1139, 2357
PspPPI	RGGWCCY	2 cut(s)	797, 2357
PspXI	VCTCGAGB	1 cut(s)	816
PstI	CTGCAG	2 cut(s)	49, 1435
PstNI	CAGNNNCTG	1 cut(s)	2981
PsuI	RGATCY	3 cut(s)	89, 2103, 2599
PvuII	CAGCTG	2 cut(s)	1436, 1904
RseI	CAYNNNNRTG	3 cut(s)	2045, 2234, 2373
SacI	GAGCTC	1 cut(s)	2782
SapI	GCTCTTC	1 cut(s)	562
SaqAI	TTAA	9 cut(s)	110, 671, 825, 849, 903, 1535, 1659, 2294, 2606
SatI	GCNGC	7 cut(s)	316, 495, 1272, 1434, 1494, 1902, 2054
Sau96I	GGNCC	7 cut(s)	619, 767, 797, 887, 1105, 1139, 2357
SchI	GAGTC	3 cut(s)	282, 799, 1660
ScrFI	CCNGG	4 cut(s)	726, 1974, 2277, 2313
SduI	GDGCHC	2 cut(s)	529, 2782
SfaNI	GCATC	9 cut(s)	484, 507, 511, 2040, 2078, 2222, 2281, 2896, 3028
SfcI	CTRYAG	3 cut(s)	45, 1431, 2484
Sfr274I	CTCGAG	1 cut(s)	816
SinI	GGWCC	6 cut(s)	619, 767, 797, 1105, 1139, 2357
SlaI	CTCGAG	1 cut(s)	816
SmiMI	CAYNNNNRTG	3 cut(s)	2045, 2234, 2373
SmlI	CTYRAG	1 cut(s)	816
SmoI	CTYRAG	1 cut(s)	816
SseBI	AGGCCT	1 cut(s)	2365
SsiI	CCGC	8 cut(s)	87, 391, 486, 1247, 1306, 1834, 2089, 3034
SspI	AATATT	1 cut(s)	2528
SspMI	CTAG	7 cut(s)	962, 1377, 2081, 2141, 2361, 3084, 3302
SstI	GAGCTC	1 cut(s)	2782
StuI	AGGCCT	1 cut(s)	2365
StyD4I	CCNGG	4 cut(s)	724, 1972, 2275, 2311
StyI	CCWWGG	5 cut(s)	1861, 2360, 2460, 2712, 3236
TaaI	ACNGT	5 cut(s)	605, 1059, 2206, 2584, 2703
TaiI	ACGT	5 cut(s)	1043, 1054, 1264, 1593, 2290
TatI	WGTACW	5 cut(s)	210, 853, 1023, 1165, 1906
Tru1I	TTAA	9 cut(s)	110, 671, 825, 849, 903, 1535, 1659, 2294, 2606
Tru9I	TTAA	9 cut(s)	110, 671, 825, 849, 903, 1535, 1659, 2294, 2606
TscAI	CASTG	3 cut(s)	375, 1531, 2587
TseFI	GTSAC	4 cut(s)	40, 2503, 3166, 3215
TseI	GCWGC	7 cut(s)	315, 494, 1271, 1433, 1493, 1901, 2053
Tsp45I	GTSAC	4 cut(s)	40, 2503, 3166, 3215
TspGWI	ACGGA	1 cut(s)	537
TspRI	CASTG	3 cut(s)	375, 1531, 2587
Van91I	CCANNNNNTGG	1 cut(s)	2675
VpaK11BI	GGWCC	6 cut(s)	619, 767, 797, 1105, 1139, 2357
XagI	CCTNNNNNAGG	2 cut(s)	816, 2814
XapI	RAATTY	4 cut(s)	176, 905, 1352, 1393
XbaI	TCTAGA	1 cut(s)	2140
XceI	RCATGY	4 cut(s)	948, 1762, 1806, 3295
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3317
XhoI	CTCGAG	1 cut(s)	816
XmaJI	CCTAGG	1 cut(s)	2360
XmiI	GTMKAC	1 cut(s)	3286
XmnI	GAANNNNTTC	5 cut(s)	180, 593, 720, 1563, 2436
XspI	CTAG	7 cut(s)	962, 1377, 2081, 2141, 2361, 3084, 3302
ZraI	GACGTC	1 cut(s)	1052

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.