Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Reverse (-)

61209681 .. 61215006

Gene Structure 5326 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G034110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2994 bp

ATGCGGAAATTGCAATTTCGGCTGTTATATCGTCAGAGGTTATATAGACTTCAGCTCTCTTATGAGAACATCTGGCAAATTGAGTTGCATCGTCCACGTGGTCACACTGCAAAATATCTTGTCATTCAGTTATATGGAGCTCCTCGCATCTACCAACAAGAGGAAGCATGTTCTTCTGGAAATGTATATGATGATCCTCTTATGAACTACTTTAGGGATGTCCCTGATGACCAATGGGTTCGGACCACTGATTTCACTCCACGAAGCTGTATTGGGGAGTCATCTTCATTATGTTTAGAGCTTCCTTCTGGTCGCCAACTTCCTGATTTTGAAGAAAATTTTGCTTATTATAAGGAGAGTGATGGGCAATTTATCTTGGAGAATGGCTCAACTTTCTCTTGCAACCGAGATCTGGTCCCTATTGTTGGTTCTCCTGCAGGGGTTGATTTGCCATATGATATCTTGTTTAAAATAAACTTGCTGGTTCAAATGGGTTGTCTATCAGGGCCGACATTGGATAATAGTTTTTATAGGCTGGTGGATCCACGAAGAATAACTCGTTCCTGTATAGAGTATGCCCTAGATAAATTATCTAATTTGAAAGAGAGCTGTTATGAACCTGCAGTGTGGCTTCATGAGGAATATCGGAAGTACCTGACATCAAAGAAACCTCCAAAGCCGGCACTTATCTCCTTAGATTCTGGTTTGGTATATGTACGCAGGGTTCAAATAACACCATCTAAAGTGTATTTCTCTGGTCCTGAGGTTAATGTCTCAAATCGCATACTACGCCAGTATTCCAGTGATATCGATGACTTTCTTCGTGTTTCATTTGTTGATGAGGAGATGGAAAAACTCTATTCAACAGATTTATCCCCCCGTACATCTACTAGGAAAACTCACATCTACAACAGAATTCTTTCTATTCTCAGAAACGGCATAGTAATTGGAGAAAAAAAATTTGAGTTTCTTGCATTCTCATCAAGTCAATTGCGAGAGAATTCGTTGTGGATGTTTGCTTCAACCTATAAAGTTTGTGCTGATAAGATAAGAGCAGGGATGGGGGATTTTCGCAAGATAAGAAACGTGGCAAAGTATGCTGCCAGATTGGGTCAATCTTTTGGCTCTTCTACTGAAACTCTAAGTGTTGCCAGGAATGAAATTGAAGTTATTCCTGATGTAACCGTTAACTGGGGTGGAGTCAAATATGTGTTCTCCGATGGGATTGGGAAAATATCAGCTGATTTTGCAAAGAGAGTAGCCTCCAAATGTGGCTATAAAGCGTTCACACCATCTGCATTTCAAATTCGATATGGTGGTTACAAAGGTGTTGTAGCTGTTGATCCAACCTCGTCAATGAAATTATCACTGAGAAAGAGCATGTCTAAGTATGATTCTGAAAACACAAAGCTAGATGTTCTAGCATGTAGCAAGTTTCAGCCTTGTTTTTTGAATCGTCAGTTGATAACTCTTCTGTCTACACTTGGAGTTCAAGATCATGTTTTCTTGAAAAAGCAAAGAGCAGCTGTACAACAACTTAATGCCATATTGACTGACCCATTAAAAGCAGAGGAGGCTCTTGATTTGATGTCTGCGGGTGAGAGTACAAACATCCTCAAGGAACTGCTGATGTGTGGTTATAAACCTGATGTTGAACCATTCCTTTCAATGATGCTCCAAACATTTCGGGCATCAAAGTTGTTGGAATTGAGGACCAAAACAAGAATCTTTATCCCAGACGGAAGAGCAATGATGGGATGTCTAGACGAAACAAAAACCTTGGAATATGGGCAGGTGTTTGTGCAAATCTCTGGAAACAGACAAAGGCAGTTATTTGATGATTCATTCATGCATAGTGGAAGCTCATCAGGCAACTGTTTTGTTATTGAGGGAAAGGTGGTTGTTGCAAAGAACCCATGTTTACACCCTGGTGATGTGCGTGTTTTAAGGGCTGTGAATGTGCCGGAGTTACACCATATGGTGGACTGTGTGGTATTTCCACAAAAAGGACCAAGGCCTCATCCAAATGAATGTTCTGGAAGTGATCTTGATGGAGATATTTACTTTGTTTGCTGGGACCCTGAGTTGATTCCTCGCAGTCAAATCCCACCTATGGATTATACCCCAGCACCAACTGTAGAGATGGATCATGATGTCACAATTGAGGAAGTAGAAGAGTACTTTGTGAATTACATACTCAATGATAGTTTGGGGATAATTGCCAATGCCCACACTGTTTTTGCTGACAGAGAGTATGGTAAAGCAACGAGCACTCCATGCATAAGGCTCGCGAAGCTATTTTCAATTGCTGTAGACTTTCCAAAAACTGGCGTGCCAGCTGTGATACCACCAGAACTGTATGTCAAGGAATATCCAGATTTCATGGAGAAGCCCGACAAAAGCACCTATCTTTCAAGCAATGTGATCGGACAGCTATTTCGTGAGGTGAAGGACATTGCACCCCATGACAGCTGTATTACATCCTTCACTCTTCAAGTGGCAACAAAATCCTATGACCCTGACATGGAGGTTGATGACTTTGAGGATTACCTTGAAGATGCTTTCTATTACAAAGGCAATTATGATTACAAATTGGGAAACCTGTTGGATTACTATGGGATCAAGACAGAGGCTGAAATATTGAGTGGGAGTATTATGAGCATGTCAAAATCTTTCACCAAGAGGCGGGATGCAGAGGCTATAAACATGGCTGTGAAGTCATTGAGGAAGGAAGCTAGGGCCTGGTTCAATGAGAAAGGAACTGGAATAGATTCTGGCGATGATGTCTATGCAAAAGCCTCAGCTTGGTATTATGTCACATATCATCCCAAATATTTTGGTTTGTACAATGAGGGAATGAAACGTGACCATTTTATCAGCTTTCCATGGTGTGTTTATGACAAGCTGGTTCACATAAAAAAGGACAAAGTAAGGCAAGCTCGGCGTATGTCATCTCTAGAAAGTCAATTGAGGTATGGCTTGCATTTGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

997

Amino Acids

113.74

Weight (kDa)

6.98

Isoelectric Point (pI)

40.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_RDR2	PF24823	2 - 124	1.2e-35	RDR2 PH-like domain
RdRP_helical	PF26252	145 - 223	8.9e-11	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	244 - 816	1e-195	RNA dependent RNA polymerase
RdRP_head	PF26253	837 - 982	8.6e-48	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	351, 1641
AarI	CACCTGC	1 cut(s)	1783
Acc36I	ACCTGC	2 cut(s)	628, 1783
AccB7I	CCANNNNNTGG	2 cut(s)	1981, 2327
AccI	GTMKAC	2 cut(s)	1478, 2313
AccII	CGCG	1 cut(s)	2291
AciI	CCGC	3 cut(s)	4, 1595, 2686
AclWI	GGATC	6 cut(s)	188, 536, 549, 1337, 2154, 2627
AcsI	RAATTY	5 cut(s)	337, 915, 959, 1000, 1305
AcuI	CTGAAG	1 cut(s)	35
AcvI	CACGTG	1 cut(s)	98
AfaI	GTAC	7 cut(s)	653, 717, 883, 1530, 1606, 2180, 2846
AjnI	CCWGG	3 cut(s)	1151, 1927, 2741
AjuI	GAANNNNNNNTTGG	2 cut(s)	2017, 2049
AleI	CACNNNNGTG	1 cut(s)	1929
Alw21I	GWGCWC	2 cut(s)	142, 2273
Alw26I	GTCTC	1 cut(s)	778
AlwI	GGATC	6 cut(s)	188, 536, 549, 1337, 2154, 2627
AlwNI	CAGNNNCTG	1 cut(s)	2633
AoxI	GGCC	3 cut(s)	506, 2015, 2739
ApeKI	GCWGC	2 cut(s)	1100, 1523
ApoI	RAATTY	5 cut(s)	337, 915, 959, 1000, 1305
ArsI	GACNNNNNNTTYG	4 cut(s)	481, 513, 2421, 2453
Asp700I	GAANNNNTTC	3 cut(s)	919, 1170, 2770
AspS9I	GGNCC	8 cut(s)	243, 415, 506, 758, 1713, 2009, 2077, 2739
AsuHPI	GGTGA	4 cut(s)	1610, 1943, 2458, 2668
AvaII	GGWCC	6 cut(s)	243, 415, 758, 1713, 2009, 2077
AxyI	CCTNAGG	1 cut(s)	762
BamHI	GGATCC	1 cut(s)	541
BanII	GRGCYC	1 cut(s)	142
BbrPI	CACGTG	1 cut(s)	98
Bbv12I	GWGCWC	2 cut(s)	142, 2273
BbvCI	CCTCAGC	1 cut(s)	2800
BbvI	GCAGC	2 cut(s)	1087, 1535
BccI	CCATC	9 cut(s)	356, 745, 841, 1054, 1214, 1300, 1747, 2045, 2137
BceAI	ACGGC	1 cut(s)	952
BcgI	CGANNNNNNTGC	4 cut(s)	2269, 2303, 2407, 2441
BciT130I	CCWGG	3 cut(s)	1153, 1929, 2743
BcoDI	GTCTC	1 cut(s)	778
BfaI	CTAG	7 cut(s)	581, 891, 1412, 1421, 1763, 2736, 2957
BfmI	CTRYAG	4 cut(s)	435, 621, 2136, 2310
BfuAI	ACCTGC	2 cut(s)	628, 1783
BglII	AGATCT	1 cut(s)	409
BisI	GCNGC	2 cut(s)	1101, 1524
BlsI	GCNGC	2 cut(s)	1102, 1525
BmcAI	AGTACT	1 cut(s)	2180
Bme1390I	CCNGG	3 cut(s)	1153, 1929, 2743
Bme18I	GGWCC	6 cut(s)	243, 415, 758, 1713, 2009, 2077
BmgT120I	GGNCC	8 cut(s)	243, 415, 506, 758, 1713, 2009, 2077, 2739
BmiI	GGNNCC	4 cut(s)	417, 543, 2078, 2079
BmrFI	CCNGG	3 cut(s)	1153, 1929, 2743
BmrI	ACTGGG	1 cut(s)	1201
BmsI	GCATC	6 cut(s)	97, 156, 1662, 1700, 2548, 2680
BmuI	ACTGGG	1 cut(s)	1201
BplI	GAGNNNNNCTC	2 cut(s)	371, 403
Bpu10I	CCTNAGC	1 cut(s)	2800
BpuEI	CTTGAG	1 cut(s)	1601
Bsa29I	ATCGAT	1 cut(s)	810
BsaAI	YACGTR	1 cut(s)	98
BsaJI	CCNNGG	4 cut(s)	1779, 1927, 2012, 2885
BsaXI	ACNNNNNCTCC	2 cut(s)	2257, 2287
Bse118I	RCCGGY	1 cut(s)	679
Bse1I	ACTGG	5 cut(s)	793, 801, 1196, 2332, 2767
Bse21I	CCTNAGG	1 cut(s)	762
Bse3DI	GCAATG	3 cut(s)	1755, 2425, 2454
BseBI	CCWGG	3 cut(s)	1153, 1929, 2743
BseCI	ATCGAT	1 cut(s)	810
BseDI	CCNNGG	4 cut(s)	1779, 1927, 2012, 2885
BseGI	GGATG	9 cut(s)	223, 1017, 1065, 1611, 1763, 2020, 2480, 2695, 2824
BseMI	GCAATG	3 cut(s)	1755, 2425, 2454
BseMII	CTCAG	5 cut(s)	753, 943, 1361, 2073, 2814
BseNI	ACTGG	5 cut(s)	793, 801, 1196, 2332, 2767
BseRI	GAGGAG	3 cut(s)	132, 857, 1586
BseXI	GCAGC	2 cut(s)	1087, 1535
BseYI	CCCAGC	2 cut(s)	2073, 2125
Bsh1236I	CGCG	1 cut(s)	2291
BshFI	GGCC	3 cut(s)	508, 2017, 2741
BshVI	ATCGAT	1 cut(s)	810
BsiHKAI	GWGCWC	2 cut(s)	142, 2273
BsiSI	CCGG	2 cut(s)	680, 1964
BslFI	GGGAC	3 cut(s)	206, 401, 2090
BsmAI	GTCTC	1 cut(s)	778
BsmFI	GGGAC	3 cut(s)	206, 401, 2090
BsmI	GAATGC	1 cut(s)	974
BsnI	GGCC	3 cut(s)	508, 2017, 2741
Bsp1286I	GDGCHC	2 cut(s)	142, 2273
Bsp1407I	TGTACA	2 cut(s)	1528, 2844
Bsp143I	GATC	9 cut(s)	193, 409, 541, 1342, 1495, 2044, 2146, 2424, 2619
Bsp19I	CCATGG	1 cut(s)	2885
Bsp68I	TCGCGA	1 cut(s)	2291
BspACI	CCGC	3 cut(s)	4, 1595, 2686
BspANI	GGCC	3 cut(s)	508, 2017, 2741
BspCNI	CTCAG	5 cut(s)	754, 942, 1362, 2074, 2813
BspDI	ATCGAT	1 cut(s)	810
BspFNI	CGCG	1 cut(s)	2291
BspHI	TCATGA	2 cut(s)	634, 2149
BspLI	GGNNCC	4 cut(s)	417, 543, 2078, 2079
BspMAI	CTGCAG	2 cut(s)	439, 625
BspMI	ACCTGC	2 cut(s)	628, 1783
BspPI	GGATC	6 cut(s)	188, 536, 549, 1337, 2154, 2627
BspQI	GCTCTTC	2 cut(s)	1132, 1738
BsrDI	GCAATG	3 cut(s)	1755, 2425, 2454
BsrFI	RCCGGY	1 cut(s)	679
BsrGI	TGTACA	2 cut(s)	1528, 2844
BsrI	ACTGG	5 cut(s)	793, 801, 1196, 2332, 2767
BssAI	RCCGGY	1 cut(s)	679
BssECI	CCNNGG	4 cut(s)	1779, 1927, 2012, 2885
BssMI	GATC	9 cut(s)	193, 409, 541, 1342, 1495, 2044, 2146, 2424, 2619
BssT1I	CCWWGG	3 cut(s)	1779, 2012, 2885
Bst2UI	CCWGG	3 cut(s)	1153, 1929, 2743
Bst4CI	ACNGT	6 cut(s)	1186, 1877, 1988, 2137, 2236, 2358
Bst6I	CTCTTC	5 cut(s)	1132, 1476, 1738, 2169, 2496
BstAPI	GCANNNNNTGC	2 cut(s)	1097, 2277
BstAUI	TGTACA	2 cut(s)	1528, 2844
BstBAI	YACGTR	1 cut(s)	98
BstC8I	GCNNGC	6 cut(s)	681, 2289, 2333, 2337, 2937, 2981
BstDEI	CTNAG	8 cut(s)	694, 762, 929, 1142, 1370, 1386, 2082, 2800
BstDSI	CCRYGG	1 cut(s)	2885
BstF5I	GGATG	9 cut(s)	223, 1017, 1065, 1611, 1763, 2020, 2480, 2695, 2824
BstFNI	CGCG	1 cut(s)	2291
BstKTI	GATC	9 cut(s)	196, 412, 544, 1345, 1498, 2047, 2149, 2427, 2622
BstMAI	GTCTC	1 cut(s)	778
BstMBI	GATC	9 cut(s)	193, 409, 541, 1342, 1495, 2044, 2146, 2424, 2619
BstNI	CCWGG	3 cut(s)	1153, 1929, 2743
BstNSI	RCATGY	4 cut(s)	171, 1384, 1428, 2665
BstSCI	CCNGG	3 cut(s)	1151, 1927, 2741
BstSFI	CTRYAG	4 cut(s)	435, 621, 2136, 2310
BstUI	CGCG	1 cut(s)	2291
BstV1I	GCAGC	2 cut(s)	1087, 1535
BstX2I	RGATCY	2 cut(s)	409, 541
BstYI	RGATCY	2 cut(s)	409, 541
Bsu15I	ATCGAT	1 cut(s)	810
Bsu36I	CCTNAGG	1 cut(s)	762
BsuRI	GGCC	3 cut(s)	508, 2017, 2741
BsuTUI	ATCGAT	1 cut(s)	810
BtgI	CCRYGG	1 cut(s)	2885
BtgZI	GCGATG	1 cut(s)	2793
BtsCI	GGATG	9 cut(s)	223, 1017, 1065, 1611, 1763, 2020, 2480, 2695, 2824
BtsI	GCAGTG	2 cut(s)	105, 630
BtsIMutI	CAGTG	6 cut(s)	105, 246, 630, 808, 1367, 2232
BtuMI	TCGCGA	1 cut(s)	2291
BveI	ACCTGC	2 cut(s)	628, 1783
Cac8I	GCNNGC	6 cut(s)	681, 2289, 2333, 2337, 2937, 2981
CaiI	CAGNNNCTG	1 cut(s)	2633
CciI	TCATGA	2 cut(s)	634, 2149
Cfr10I	RCCGGY	1 cut(s)	679
Cfr13I	GGNCC	8 cut(s)	243, 415, 506, 758, 1713, 2009, 2077, 2739
ClaI	ATCGAT	1 cut(s)	810
Csp6I	GTAC	7 cut(s)	652, 716, 882, 1529, 1605, 2179, 2845
CviQI	GTAC	7 cut(s)	652, 716, 882, 1529, 1605, 2179, 2845
DdeI	CTNAG	8 cut(s)	694, 762, 929, 1142, 1370, 1386, 2082, 2800
DpnI	GATC	9 cut(s)	195, 411, 543, 1344, 1497, 2046, 2148, 2426, 2621
DpnII	GATC	9 cut(s)	193, 409, 541, 1342, 1495, 2044, 2146, 2424, 2619
DraI	TTTAAA	1 cut(s)	469
Eam1104I	CTCTTC	5 cut(s)	1132, 1476, 1738, 2169, 2496
EarI	CTCTTC	5 cut(s)	1132, 1476, 1738, 2169, 2496
Ecl136II	GAGCTC	1 cut(s)	140
Eco130I	CCWWGG	3 cut(s)	1779, 2012, 2885
Eco147I	AGGCCT	1 cut(s)	2017
Eco24I	GRGCYC	1 cut(s)	142
Eco32I	GATATC	2 cut(s)	460, 808
Eco47I	GGWCC	6 cut(s)	243, 415, 758, 1713, 2009, 2077
Eco53kI	GAGCTC	1 cut(s)	140
Eco57I	CTGAAG	1 cut(s)	35
Eco72I	CACGTG	1 cut(s)	98
Eco81I	CCTNAGG	1 cut(s)	762
EcoICRI	GAGCTC	1 cut(s)	140
EcoO109I	RGGNCCY	2 cut(s)	2077, 2739
EcoRI	GAATTC	2 cut(s)	915, 1000
EcoRII	CCWGG	3 cut(s)	1151, 1927, 2741
EcoRV	GATATC	2 cut(s)	460, 808
EcoT14I	CCWWGG	3 cut(s)	1779, 2012, 2885
EcoT22I	ATGCAT	2 cut(s)	1854, 2282
EcoT38I	GRGCYC	1 cut(s)	142
ErhI	CCWWGG	3 cut(s)	1779, 2012, 2885
FaqI	GGGAC	3 cut(s)	206, 401, 2090
FauI	CCCGC	2 cut(s)	1588, 2679
FauNDI	CATATG	2 cut(s)	454, 1977
FblI	GTMKAC	2 cut(s)	1478, 2313
Fnu4HI	GCNGC	2 cut(s)	1101, 1524
FokI	GGATG	9 cut(s)	230, 1024, 1072, 1598, 1770, 2007, 2467, 2702, 2811
FriOI	GRGCYC	1 cut(s)	142
Fsp4HI	GCNGC	2 cut(s)	1101, 1524
FspBI	CTAG	7 cut(s)	581, 891, 1412, 1421, 1763, 2736, 2957
GluI	GCNGC	2 cut(s)	1101, 1524
GsaI	CCCAGC	2 cut(s)	2077, 2129
HaeIII	GGCC	3 cut(s)	508, 2017, 2741
HapII	CCGG	2 cut(s)	680, 1964
HincII	GTYRAC	1 cut(s)	1189
HindII	GTYRAC	1 cut(s)	1189
HinfI	GANTC	9 cut(s)	278, 698, 1200, 1394, 1453, 1725, 1841, 2089, 2771
HpaI	GTTAAC	1 cut(s)	1189
HpaII	CCGG	2 cut(s)	680, 1964
HphI	GGTGA	4 cut(s)	1610, 1943, 2458, 2668
Hpy166II	GTNNAC	8 cut(s)	95, 1189, 1287, 1479, 1922, 1984, 2314, 2911
Hpy188I	TCNGA	8 cut(s)	36, 243, 648, 932, 1219, 1399, 2429, 2993
Hpy8I	GTNNAC	8 cut(s)	95, 1189, 1287, 1479, 1922, 1984, 2314, 2911
HpyAV	CCTTC	4 cut(s)	315, 2443, 2494, 2722
HpyCH4III	ACNGT	6 cut(s)	1186, 1877, 1988, 2137, 2236, 2358
HpyCH4IV	ACGT	3 cut(s)	97, 1086, 2863
HpyF3I	CTNAG	8 cut(s)	694, 762, 929, 1142, 1370, 1386, 2082, 2800
HpySE526I	ACGT	3 cut(s)	97, 1086, 2863
KflI	GGGWCCC	1 cut(s)	2077
KroI	GCCGGC	1 cut(s)	679
KroNI	GCCGGC	1 cut(s)	681
KspAI	GTTAAC	1 cut(s)	1189
Kzo9I	GATC	9 cut(s)	193, 409, 541, 1342, 1495, 2044, 2146, 2424, 2619
LguI	GCTCTTC	2 cut(s)	1132, 1738
LmnI	GCTCC	3 cut(s)	137, 145, 1680
Lsp1109I	GCAGC	2 cut(s)	1087, 1535
LweI	GCATC	6 cut(s)	97, 156, 1662, 1700, 2548, 2680
MaeI	CTAG	7 cut(s)	581, 891, 1412, 1421, 1763, 2736, 2957
MaeII	ACGT	3 cut(s)	97, 1086, 2863
MaeIII	GTNAC	7 cut(s)	101, 1180, 1319, 1968, 2155, 2815, 2864
MalI	GATC	9 cut(s)	195, 411, 543, 1344, 1497, 2046, 2148, 2426, 2621
MboI	GATC	9 cut(s)	193, 409, 541, 1342, 1495, 2044, 2146, 2424, 2619
MfeI	CAATTG	4 cut(s)	989, 2160, 2304, 2966
MflI	RGATCY	2 cut(s)	409, 541
MhlI	GDGCHC	2 cut(s)	142, 2273
MlyI	GAGTC	2 cut(s)	287, 1209
MmeI	TCCRAC	3 cut(s)	1370, 1683, 2586
Mph1103I	ATGCAT	2 cut(s)	1854, 2282
MroNI	GCCGGC	1 cut(s)	679
MroXI	GAANNNNTTC	3 cut(s)	919, 1170, 2770
MseI	TTAA	6 cut(s)	468, 768, 1188, 1539, 1562, 1946
MslI	CAYNNNNRTG	3 cut(s)	1929, 2025, 2711
MspA1I	CMGCKG	4 cut(s)	1241, 1526, 2339, 2472
MspI	CCGG	2 cut(s)	680, 1964
MspR9I	CCNGG	3 cut(s)	1153, 1929, 2743
MunI	CAATTG	4 cut(s)	989, 2160, 2304, 2966
Mva1269I	GAATGC	1 cut(s)	974
MvaI	CCWGG	3 cut(s)	1153, 1929, 2743
MvnI	CGCG	1 cut(s)	2291
NaeI	GCCGGC	1 cut(s)	681
NcoI	CCATGG	1 cut(s)	2885
NdeI	CATATG	2 cut(s)	454, 1977
NdeII	GATC	9 cut(s)	193, 409, 541, 1342, 1495, 2044, 2146, 2424, 2619
NgoMIV	GCCGGC	1 cut(s)	679
NlaIV	GGNNCC	4 cut(s)	417, 543, 2078, 2079
NmeAIII	GCCGAG	1 cut(s)	2920
NmuCI	GTSAC	4 cut(s)	101, 2155, 2815, 2864
NruI	TCGCGA	1 cut(s)	2291
NsiI	ATGCAT	2 cut(s)	1854, 2282
NspI	RCATGY	4 cut(s)	171, 1384, 1428, 2665
OliI	CACNNNNGTG	1 cut(s)	1929
PagI	TCATGA	2 cut(s)	634, 2149
PaqCI	CACCTGC	1 cut(s)	1783
PceI	AGGCCT	1 cut(s)	2017
PciSI	GCTCTTC	2 cut(s)	1132, 1738
PctI	GAATGC	1 cut(s)	974
PdiI	GCCGGC	1 cut(s)	681
PdmI	GAANNNNTTC	3 cut(s)	919, 1170, 2770
PfeI	GAWTC	7 cut(s)	698, 1394, 1453, 1725, 1841, 2089, 2771
PflMI	CCANNNNNTGG	2 cut(s)	1981, 2327
PkrI	GCNGC	2 cut(s)	1102, 1525
PleI	GAGTC	2 cut(s)	286, 1208
PmaCI	CACGTG	1 cut(s)	98
PmlI	CACGTG	1 cut(s)	98
PpsI	GAGTC	2 cut(s)	286, 1208
Ppu21I	YACGTR	1 cut(s)	98
PpuMI	RGGWCCY	1 cut(s)	2077
PsiI	TTATAA	2 cut(s)	351, 1641
Psp124BI	GAGCTC	1 cut(s)	142
Psp5II	RGGWCCY	1 cut(s)	2077
Psp6I	CCWGG	3 cut(s)	1151, 1927, 2741
PspCI	CACGTG	1 cut(s)	98
PspFI	CCCAGC	2 cut(s)	2073, 2125
PspGI	CCWGG	3 cut(s)	1151, 1927, 2741
PspN4I	GGNNCC	4 cut(s)	417, 543, 2078, 2079
PspPI	GGNCC	8 cut(s)	243, 415, 506, 758, 1713, 2009, 2077, 2739
PspPPI	RGGWCCY	1 cut(s)	2077
PsrI	GAACNNNNNNTAC	2 cut(s)	708, 740
PstI	CTGCAG	2 cut(s)	439, 625
PstNI	CAGNNNCTG	1 cut(s)	2633
PsuI	RGATCY	2 cut(s)	409, 541
PvuII	CAGCTG	4 cut(s)	1241, 1526, 2339, 2472
RruI	TCGCGA	1 cut(s)	2291
RsaI	GTAC	7 cut(s)	653, 717, 883, 1530, 1606, 2180, 2846
RsaNI	GTAC	7 cut(s)	652, 716, 882, 1529, 1605, 2179, 2845
RseI	CAYNNNNRTG	3 cut(s)	1929, 2025, 2711
SacI	GAGCTC	1 cut(s)	142
SapI	GCTCTTC	2 cut(s)	1132, 1738
SaqAI	TTAA	6 cut(s)	468, 768, 1188, 1539, 1562, 1946
SatI	GCNGC	2 cut(s)	1101, 1524
Sau3AI	GATC	9 cut(s)	193, 409, 541, 1342, 1495, 2044, 2146, 2424, 2619
Sau96I	GGNCC	8 cut(s)	243, 415, 506, 758, 1713, 2009, 2077, 2739
SbfI	CCTGCAGG	1 cut(s)	439
ScaI	AGTACT	1 cut(s)	2180
SchI	GAGTC	2 cut(s)	287, 1209
ScrFI	CCNGG	3 cut(s)	1153, 1929, 2743
SdaI	CCTGCAGG	1 cut(s)	439
SduI	GDGCHC	2 cut(s)	142, 2273
SfaNI	GCATC	6 cut(s)	97, 156, 1662, 1700, 2548, 2680
SfcI	CTRYAG	4 cut(s)	435, 621, 2136, 2310
SinI	GGWCC	6 cut(s)	243, 415, 758, 1713, 2009, 2077
SmiMI	CAYNNNNRTG	3 cut(s)	1929, 2025, 2711
SmlI	CTYRAG	1 cut(s)	1616
SmoI	CTYRAG	1 cut(s)	1616
Sse8387I	CCTGCAGG	1 cut(s)	439
SseBI	AGGCCT	1 cut(s)	2017
SsiI	CCGC	3 cut(s)	4, 1595, 2686
SspI	AATATT	2 cut(s)	2640, 2834
SspMI	CTAG	7 cut(s)	581, 891, 1412, 1421, 1763, 2736, 2957
SstI	GAGCTC	1 cut(s)	142
StuI	AGGCCT	1 cut(s)	2017
StyD4I	CCNGG	3 cut(s)	1151, 1927, 2741
StyI	CCWWGG	3 cut(s)	1779, 2012, 2885
TaaI	ACNGT	6 cut(s)	1186, 1877, 1988, 2137, 2236, 2358
TaiI	ACGT	3 cut(s)	100, 1089, 2866
TaqI	TCGA	2 cut(s)	810, 1309
TatI	WGTACW	4 cut(s)	1528, 1604, 2178, 2844
TfiI	GAWTC	7 cut(s)	698, 1394, 1453, 1725, 1841, 2089, 2771
Tru1I	TTAA	6 cut(s)	468, 768, 1188, 1539, 1562, 1946
Tru9I	TTAA	6 cut(s)	468, 768, 1188, 1539, 1562, 1946
TscAI	CASTG	6 cut(s)	112, 253, 630, 808, 1374, 2239
TseFI	GTSAC	4 cut(s)	101, 2155, 2815, 2864
TseI	GCWGC	2 cut(s)	1100, 1523
Tsp45I	GTSAC	4 cut(s)	101, 2155, 2815, 2864
TspGWI	ACGGA	1 cut(s)	1755
TspRI	CASTG	6 cut(s)	112, 253, 630, 808, 1374, 2239
Van91I	CCANNNNNTGG	2 cut(s)	1981, 2327
VpaK11BI	GGWCC	6 cut(s)	243, 415, 758, 1713, 2009, 2077
XapI	RAATTY	5 cut(s)	337, 915, 959, 1000, 1305
XbaI	TCTAGA	2 cut(s)	1762, 2956
XceI	RCATGY	4 cut(s)	171, 1384, 1428, 2665
XmiI	GTMKAC	2 cut(s)	1478, 2313
XmnI	GAANNNNTTC	3 cut(s)	919, 1170, 2770
XspI	CTAG	7 cut(s)	581, 891, 1412, 1421, 1763, 2736, 2957
ZrmI	AGTACT	1 cut(s)	2180
Zsp2I	ATGCAT	2 cut(s)	1854, 2282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.