Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Reverse (-)

61222099 .. 61226786

Gene Structure 4688 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G034130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3375 bp

ATGGAGAAGACAATAAAATTACATGGATTCCCTTCAGGTGAATCTGCTGAAAAAATTAAGGTATTTTTGGAGAAACATACAGGAGAAGGAAGTGTTTATGCTCTTAAATTACGGCCGGCCAAAGGGGGAAGATTCTATGCTTTTATACAGTTTGAGTCCACCAGTCAGGCTGAGCTTATCACCTCTTTGGCTAAAGTTGGGCTGTGGTATGGGAGATCATATTTGAAAGCTTATCCAGCGGACCATGATATTGTACCAAAGCCAAGAACTGTAATGCATAGCTTGCAAAATTTAGTATTGCATGTTGGCTGCCAGGTTGCAAATGATAAGTTGTCTGTTTTTTGGAGAACGGCTGAAGTTTCAGTGGACTTTGGGACTGGAACGCGGAAATTCCAATTTCGGCTGTTATATCATCAGAAGTTATATAGACTTCAGCTTTCGTATGAGAACATCTGGCAAATTGAGTTGCATCGTCCACGTGGTCGCACTGCAAAATATCTTGTCATTCAGTTATATGGAGCTCCTCGCATCTACCAACAAGAAGAAGCATGTTCTTCTGGAAATGTATATGATGACCCTCTTATGAACTACTTTAGGGATGTCCCTGATGACCAATGGGTTCGGACCACTGATTTCACTCCACGAAGCTGTATTGGGGAGTCATCTTCATTATGTTTAGAGCTTCCTTCTGGTCGCCAACTTCCTGATTTTGAAGAAAATTTTGCTTATTATAAGGAGAGTGATGGGCAATTTATCTTGGAGAATGGCTCAACTTTCTCTTGCAACCGAGATCTGGTCCCTATTGTTGGTTCTCCTGCAGGGGTTGATTTGCCATATGATATCTTGTTTAAAATAAACTCGCTGGTTCAAATGGGTTGTCTGTCAGGGCCGACACTTGATAATAGGTTTTATAGACTGGTGGATCCACGAAGAATAACTCGTTCCTGTATAGAGTATGCCCTAGATAAATTATCTAATTTGAAAAAGAGCTGTTATGAACCTGCGGTGTGGCTTAATGAGGAGTACCGGAAGTACCTGACTACAAAGAAACCTCCAAAGCCGGCACTTGTCTCCTTAGATTCTGGTTTGGTATATGTACGGAGGGTTCAAATAACACCATCTAAAGTGTATTTTTCTGGTCCTGAGGTTAATGTCTCAAATCGCATACTACGTCAGTATGCCAGTGATATCGATAACTTTCTTCGTGTTTCATTTGTTGATGAGGAGATGGAAAATCTCTATTCAACAGATTTGTCCCCCCGTACATCTACTAGAAAAACTCGCATCTACAACAGAGTTCTTTCTATTCTCAGAAACGGCATAGTAATTGGAGAAAAGAAATTTGAGTTTCTTGCATTCTCATCAAGTCAATTGCAAGAGAATTCATTGTGGATGTTTTCTTCAACCTCTAGTGTTAGTGCTGATAAGATAAGAGCAGGGATGGGGGATTTTCGCAAGATAAGAAATGTGGCAAAGTATGCTGCCAGATTGGGTCAATCTTTTGGCTCTTCTACTGAAACTCTAAGTGTTGGCAGGAATGAGATTGAAGTTATTCCTGATGTAACAGTTAATTGGGGTGGAGTCAAATATGTGTTCTCCGATGGGATTGGGAAAATATCAGCTGATTTTGCAAAGAGAGTAGCCTCCAAATGTGGCTATAAAGCGTTCACACCATCTGCATTTCAAATTCGATATGGTGGTTACAAAGGTGTTGTAGCTGTTGATCCAACCTCGTCAATGAAATTATCACTGAGAAAGAGCATGTCTAAGTATGATTCTGAAAACACAAAGCTAGATGTTCTAGCATGTAGCAAGTTTCAGCCTTGTTTTTTGAATCGTCAGGTGATAACTCTTCTGTCTACACTTGGAGTTCAAGATCATGTTTTCTTGAAAAAGCAAAGAGCAGCTGTACAACAACTTAATGCCATATTGACTGACCCATTAAAAGCAGGGGAGGCTCTTGATTTGATGTCTGCGAGTGAGTGTACAAACATCCTCAAGGAACTGCTGATGTGTGGTTATAAACCTGATGTTGAACCATTCCTTTCAATGATGCTCCAAACATTTCGGGCATCAAAGTTGTTGGAATTGAGGACCAAAACAAGAATCTTTATCCCAGATGGAAGAGCAATGATGGGATGTCTGGATGAAACAAGAACCTTGGAATATGGGCAGGTGTTTGTGCAAATCTGTGGAAACAGACGAAGGCAGTTATTTGATGATTCATTCATGCATAGTGGAAGCTCATCAGGCAAAACAGTTATTGAAGGAAAGGTGGTTGTTGCAAAGAACCCATGTTTACACCCTGGTGATGTGCGTGTCTTAAGGGCTGTGAATGTGCCGGAGTTACGACATATGGTGGACTGTGTGGTATTTCCACAAAAAGGACCAAGGCCTCATCCAAATGAATGTTCTGGAAGTGATCTTGATGGAGATATTTACTTTGTTTGCTGGGACCCCGAGTTGATTCCTCGCAAGCAAATCCAACCTATGGATTATACCCCAGCACCAACTATAGAGATGGATCATGATGTCACAATTGAGGAAGTAGAAGAGCACTTTGTGAATTACATACTCAATGATAGTTTGGGGAGAATTGCCAATGCCCACACTGTTTTTGCTGACAGAGAGCATGATAAAGCAATGAGCCTTCCGTGCATAGAGCTCGCGAAGCTACATTCAATTGCTGTAGACTTCCCAAAAACTGGCGTGCCAGCTGTGAGACCAAAGGAACTGTATGTCAAGGAATATCCAGATTTCATGGAGAAGACCGACAAAAGCACCTATCTCTCAAGCAATGTGATCGGACAGCTTTTTCGTGAGGTGAAGAACATTGCACCCCATGACAGCTGTATTACATCCTTCACTCATCAAGTGGCGACAAAATCCTATGACCCTGACATGGAGGTTGATGGCTTTGAGGATTACCTTGAAGATGCTTTCTATTACAAAGGCAATTATGATTACAAATTGGGAAACCTGTTGGATTACTATGGGATCAAGACAGAGGCCGAAATATTGAGTGGGGGTATTATGAGAATGTCAAAATCTTTCACCAAAGAGCGGGATGCAGACGCTATAAACAGGGCTGTGAGGTCATTGAGGAAGGAAGCTAGGACCTGGTTCGATGAGAAAGGAACTGGAATAGATTCTGGAGTTGATGGTGTCTACGCAAAAGCTTCAGCTTGGTACCATGTCACATATCATCCCAATTATTTTGGTTTGTACAATGAGGGAATGAAACGTGACCATTTTATCAGCTTCCCGTGGTGTGTGTATGACAAGCTAGTTCACATAAAAAAGGACAAAGTAAAGCAAGCTCGGCGCAGATCATCTCTAGAAAGTCAGTTCTGGTACGGCTTGCATTTGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

1124

Amino Acids

127.91

Weight (kDa)

8.54

Isoelectric Point (pI)

40.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_RdRP1_2	PF26250	4 - 82	4.7e-14	RdRP1/2, RRM domain
RDR6_2nd	PF24577	96 - 246	8.6e-06	RNA-dependent RNA polymerase 6 second domain
PH_RDR2	PF24823	100 - 251	6.6e-45	RDR2 PH-like domain
RdRP_helical	PF26252	272 - 346	2.5e-12	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	371 - 942	1.6e-195	RNA dependent RNA polymerase
RdRP_head	PF26253	963 - 1110	4.4e-47	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	732, 2022
AarI	CACCTGC	1 cut(s)	2164
Acc36I	ACCTGC	2 cut(s)	1009, 2164
Acc65I	GGTACC	1 cut(s)	3189
AccB1I	GGYRCC	1 cut(s)	3189
AccB7I	CCANNNNNTGG	2 cut(s)	2491, 2705
AccBSI	CCGCTC	1 cut(s)	3064
AccI	GTMKAC	3 cut(s)	1859, 2691, 3168
AccII	CGCG	2 cut(s)	385, 2669
AciI	CCGC	4 cut(s)	239, 385, 1004, 3064
AclWI	GGATC	5 cut(s)	917, 930, 1718, 2532, 3005
AcoI	YGGCCR	2 cut(s)	113, 117
AcsI	RAATTY	6 cut(s)	289, 389, 718, 1340, 1381, 1686
AcuI	CTGAAG	4 cut(s)	18, 375, 416, 3165
AcvI	CACGTG	1 cut(s)	479
AdeI	CACNNNGTG	1 cut(s)	2563
AfiI	CCNNNNNNNGG	8 cut(s)	122, 793, 806, 2384, 2491, 2705, 2902, 3063
AflII	CTTAAG	1 cut(s)	2323
AjnI	CCWGG	3 cut(s)	312, 2305, 3119
AjuI	GAANNNNNNNTTGG	2 cut(s)	2395, 2427
AleI	CACNNNNGTG	1 cut(s)	2307
Alw21I	GWGCWC	3 cut(s)	523, 2559, 2667
Alw26I	GTCTC	3 cut(s)	1075, 1159, 2716
AlwI	GGATC	5 cut(s)	917, 930, 1718, 2532, 3005
Ama87I	CYCGRG	1 cut(s)	2459
AoxI	GGCC	5 cut(s)	113, 117, 887, 2393, 3009
ApeKI	GCWGC	3 cut(s)	309, 1481, 1904
ApoI	RAATTY	6 cut(s)	289, 389, 718, 1340, 1381, 1686
ArsI	GACNNNNNNTTYG	4 cut(s)	862, 894, 2799, 2831
Asp700I	GAANNNNTTC	2 cut(s)	1551, 3148
Asp718I	GGTACC	1 cut(s)	3189
AspLEI	GCGC	1 cut(s)	3327
AspS9I	GGNCC	9 cut(s)	241, 624, 796, 887, 1139, 2094, 2387, 2455, 3117
AsuHPI	GGTGA	6 cut(s)	50, 172, 1855, 2321, 2836, 3046
AvaI	CYCGRG	1 cut(s)	2459
AvaII	GGWCC	8 cut(s)	241, 624, 796, 1139, 2094, 2387, 2455, 3117
AxyI	CCTNAGG	1 cut(s)	1143
BamHI	GGATCC	1 cut(s)	922
BanI	GGYRCC	1 cut(s)	3189
BanII	GRGCYC	2 cut(s)	523, 2667
BbrPI	CACGTG	1 cut(s)	479
BbsI	GAAGAC	2 cut(s)	14, 2774
Bbv12I	GWGCWC	3 cut(s)	523, 2559, 2667
BbvI	GCAGC	3 cut(s)	296, 1468, 1916
BceAI	ACGGC	4 cut(s)	128, 366, 1333, 3373
BcgI	CGANNNNNNTGC	4 cut(s)	2647, 2681, 2785, 2819
BciT130I	CCWGG	3 cut(s)	314, 2307, 3121
BcoDI	GTCTC	3 cut(s)	1075, 1159, 2716
BfaI	CTAG	8 cut(s)	962, 1272, 1410, 1793, 1802, 3114, 3287, 3338
BfmI	CTRYAG	3 cut(s)	816, 2514, 2688
BfrI	CTTAAG	1 cut(s)	2323
BfuAI	ACCTGC	2 cut(s)	1009, 2164
BglII	AGATCT	1 cut(s)	790
BisI	GCNGC	3 cut(s)	310, 1482, 1905
BlpI	GCTNAGC	1 cut(s)	171
BlsI	GCNGC	3 cut(s)	311, 1483, 1906
Bme1390I	CCNGG	3 cut(s)	314, 2307, 3121
Bme18I	GGWCC	8 cut(s)	241, 624, 796, 1139, 2094, 2387, 2455, 3117
BmeT110I	CYCGRG	1 cut(s)	2459
BmgT120I	GGNCC	9 cut(s)	241, 624, 796, 887, 1139, 2094, 2387, 2455, 3117
BmiI	GGNNCC	5 cut(s)	798, 924, 2456, 2457, 3191
BmrFI	CCNGG	3 cut(s)	314, 2307, 3121
BmsI	GCATC	7 cut(s)	478, 537, 1293, 2043, 2081, 2926, 3058
BpiI	GAAGAC	2 cut(s)	14, 2774
BplI	GAGNNNNNCTC	2 cut(s)	752, 784
BpmI	CTGGAG	1 cut(s)	3174
Bpu1102I	GCTNAGC	1 cut(s)	171
BpuEI	CTTGAG	2 cut(s)	1982, 2776
Bsa29I	ATCGAT	1 cut(s)	1191
BsaAI	YACGTR	1 cut(s)	479
BsaI	GGTCTC	1 cut(s)	2716
BsaJI	CCNNGG	4 cut(s)	2160, 2305, 2390, 3266
BsaWI	WCCGGW	1 cut(s)	1026
Bsc4I	CCNNNNNNNGG	8 cut(s)	122, 793, 806, 2384, 2491, 2705, 2902, 3063
Bse118I	RCCGGY	2 cut(s)	115, 1060
Bse1I	ACTGG	6 cut(s)	162, 382, 921, 1182, 2710, 3145
Bse21I	CCTNAGG	1 cut(s)	1143
Bse3DI	GCAATG	4 cut(s)	2136, 2649, 2803, 2832
BseBI	CCWGG	3 cut(s)	314, 2307, 3121
BseCI	ATCGAT	1 cut(s)	1191
BseDI	CCNNGG	4 cut(s)	2160, 2305, 2390, 3266
BseLI	CCNNNNNNNGG	8 cut(s)	122, 793, 806, 2384, 2491, 2705, 2902, 3063
BseMI	GCAATG	4 cut(s)	2136, 2649, 2803, 2832
BseMII	CTCAG	4 cut(s)	162, 1134, 1324, 1742
BseNI	ACTGG	6 cut(s)	162, 382, 921, 1182, 2710, 3145
BseRI	GAGGAG	3 cut(s)	513, 1034, 1238
BseX3I	CGGCCG	1 cut(s)	113
BseXI	GCAGC	3 cut(s)	296, 1468, 1916
BseYI	CCCAGC	2 cut(s)	2451, 2503
Bsh1236I	CGCG	2 cut(s)	385, 2669
Bsh1285I	CGRYCG	1 cut(s)	116
BshFI	GGCC	5 cut(s)	115, 119, 889, 2395, 3011
BshNI	GGYRCC	1 cut(s)	3189
BshVI	ATCGAT	1 cut(s)	1191
BsiEI	CGRYCG	1 cut(s)	116
BsiHKAI	GWGCWC	3 cut(s)	523, 2559, 2667
BsiHKCI	CYCGRG	1 cut(s)	2459
BsiSI	CCGG	4 cut(s)	116, 1027, 1061, 2342
BslFI	GGGAC	5 cut(s)	388, 587, 782, 1240, 2468
BslI	CCNNNNNNNGG	8 cut(s)	122, 793, 806, 2384, 2491, 2705, 2902, 3063
BsmAI	GTCTC	3 cut(s)	1075, 1159, 2716
BsmFI	GGGAC	5 cut(s)	388, 587, 782, 1240, 2468
BsmI	GAATGC	1 cut(s)	1355
BsnI	GGCC	5 cut(s)	115, 119, 889, 2395, 3011
Bso31I	GGTCTC	1 cut(s)	2716
BsoBI	CYCGRG	1 cut(s)	2459
Bsp1286I	GDGCHC	3 cut(s)	523, 2559, 2667
Bsp1407I	TGTACA	3 cut(s)	1909, 1985, 3225
Bsp1720I	GCTNAGC	1 cut(s)	171
Bsp68I	TCGCGA	1 cut(s)	2669
BspACI	CCGC	4 cut(s)	239, 385, 1004, 3064
BspANI	GGCC	5 cut(s)	115, 119, 889, 2395, 3011
BspCNI	CTCAG	4 cut(s)	163, 1135, 1323, 1743
BspDI	ATCGAT	1 cut(s)	1191
BspFNI	CGCG	2 cut(s)	385, 2669
BspHI	TCATGA	1 cut(s)	2527
BspLI	GGNNCC	5 cut(s)	798, 924, 2456, 2457, 3191
BspMAI	CTGCAG	1 cut(s)	820
BspMI	ACCTGC	2 cut(s)	1009, 2164
BspPI	GGATC	5 cut(s)	917, 930, 1718, 2532, 3005
BspQI	GCTCTTC	3 cut(s)	1513, 2119, 2547
BspT107I	GGYRCC	1 cut(s)	3189
BspTI	CTTAAG	1 cut(s)	2323
BspTNI	GGTCTC	1 cut(s)	2716
BsrBI	CCGCTC	1 cut(s)	3064
BsrDI	GCAATG	4 cut(s)	2136, 2649, 2803, 2832
BsrFI	RCCGGY	2 cut(s)	115, 1060
BsrGI	TGTACA	3 cut(s)	1909, 1985, 3225
BsrI	ACTGG	6 cut(s)	162, 382, 921, 1182, 2710, 3145
BssAI	RCCGGY	2 cut(s)	115, 1060
BssECI	CCNNGG	4 cut(s)	2160, 2305, 2390, 3266
BssT1I	CCWWGG	2 cut(s)	2160, 2390
Bst2UI	CCWGG	3 cut(s)	314, 2307, 3121
Bst4CI	ACNGT	7 cut(s)	150, 271, 1567, 2260, 2366, 2614, 2736
Bst6I	CTCTTC	4 cut(s)	1513, 1857, 2119, 2547
BstAFI	CTTAAG	1 cut(s)	2323
BstAPI	GCANNNNNTGC	2 cut(s)	283, 1478
BstAUI	TGTACA	3 cut(s)	1909, 1985, 3225
BstBAI	YACGTR	1 cut(s)	479
BstC8I	GCNNGC	9 cut(s)	117, 284, 1062, 2477, 2667, 2711, 2715, 3318, 3362
BstDEI	CTNAG	7 cut(s)	171, 1075, 1143, 1310, 1523, 1751, 1767
BstDSI	CCRYGG	1 cut(s)	3266
BstFNI	CGCG	2 cut(s)	385, 2669
BstHHI	GCGC	1 cut(s)	3327
BstMAI	GTCTC	3 cut(s)	1075, 1159, 2716
BstMCI	CGRYCG	1 cut(s)	116
BstMWI	GCNNNNNNNGC	9 cut(s)	236, 283, 1478, 1628, 1955, 2250, 2655, 2671, 3322
BstNI	CCWGG	3 cut(s)	314, 2307, 3121
BstNSI	RCATGY	4 cut(s)	305, 552, 1765, 1809
BstSCI	CCNGG	3 cut(s)	312, 2305, 3119
BstSFI	CTRYAG	3 cut(s)	816, 2514, 2688
BstUI	CGCG	2 cut(s)	385, 2669
BstV1I	GCAGC	3 cut(s)	296, 1468, 1916
BstV2I	GAAGAC	2 cut(s)	14, 2774
BstX2I	RGATCY	2 cut(s)	790, 922
BstYI	RGATCY	2 cut(s)	790, 922
BstZI	CGGCCG	1 cut(s)	113
Bsu15I	ATCGAT	1 cut(s)	1191
Bsu36I	CCTNAGG	1 cut(s)	1143
BsuRI	GGCC	5 cut(s)	115, 119, 889, 2395, 3011
BsuTUI	ATCGAT	1 cut(s)	1191
BtgI	CCRYGG	1 cut(s)	3266
BtsI	GCAGTG	1 cut(s)	486
BtsIMutI	CAGTG	6 cut(s)	369, 486, 627, 1189, 1748, 2610
BtuMI	TCGCGA	1 cut(s)	2669
BveI	ACCTGC	2 cut(s)	1009, 2164
Cac8I	GCNNGC	9 cut(s)	117, 284, 1062, 2477, 2667, 2711, 2715, 3318, 3362
CciI	TCATGA	1 cut(s)	2527
CfoI	GCGC	1 cut(s)	3327
Cfr10I	RCCGGY	2 cut(s)	115, 1060
Cfr13I	GGNCC	9 cut(s)	241, 624, 796, 887, 1139, 2094, 2387, 2455, 3117
ClaI	ATCGAT	1 cut(s)	1191
CseI	GACGC	1 cut(s)	3083
CsiI	ACCWGGT	1 cut(s)	3119
DdeI	CTNAG	7 cut(s)	171, 1075, 1143, 1310, 1523, 1751, 1767
DraI	TTTAAA	1 cut(s)	850
DraIII	CACNNNGTG	1 cut(s)	2563
EaeI	YGGCCR	2 cut(s)	113, 117
EagI	CGGCCG	1 cut(s)	113
Eam1104I	CTCTTC	4 cut(s)	1513, 1857, 2119, 2547
EarI	CTCTTC	4 cut(s)	1513, 1857, 2119, 2547
Ecl136II	GAGCTC	2 cut(s)	521, 2665
EclXI	CGGCCG	1 cut(s)	113
Eco130I	CCWWGG	2 cut(s)	2160, 2390
Eco147I	AGGCCT	1 cut(s)	2395
Eco24I	GRGCYC	2 cut(s)	523, 2667
Eco31I	GGTCTC	1 cut(s)	2716
Eco32I	GATATC	2 cut(s)	841, 1189
Eco47I	GGWCC	8 cut(s)	241, 624, 796, 1139, 2094, 2387, 2455, 3117
Eco52I	CGGCCG	1 cut(s)	113
Eco53kI	GAGCTC	2 cut(s)	521, 2665
Eco57I	CTGAAG	4 cut(s)	18, 375, 416, 3165
Eco72I	CACGTG	1 cut(s)	479
Eco81I	CCTNAGG	1 cut(s)	1143
Eco88I	CYCGRG	1 cut(s)	2459
EcoICRI	GAGCTC	2 cut(s)	521, 2665
EcoO109I	RGGNCCY	2 cut(s)	2455, 3117
EcoRI	GAATTC	1 cut(s)	1381
EcoRII	CCWGG	3 cut(s)	312, 2305, 3119
EcoRV	GATATC	2 cut(s)	841, 1189
EcoT14I	CCWWGG	2 cut(s)	2160, 2390
EcoT22I	ATGCAT	2 cut(s)	279, 2235
EcoT38I	GRGCYC	2 cut(s)	523, 2667
ErhI	CCWWGG	2 cut(s)	2160, 2390
FaqI	GGGAC	5 cut(s)	388, 587, 782, 1240, 2468
FauI	CCCGC	1 cut(s)	3057
FauNDI	CATATG	2 cut(s)	835, 2355
FblI	GTMKAC	3 cut(s)	1859, 2691, 3168
Fnu4HI	GCNGC	3 cut(s)	310, 1482, 1905
FriOI	GRGCYC	2 cut(s)	523, 2667
FseI	GGCCGGCC	1 cut(s)	119
Fsp4HI	GCNGC	3 cut(s)	310, 1482, 1905
FspBI	CTAG	8 cut(s)	962, 1272, 1410, 1793, 1802, 3114, 3287, 3338
GlaI	GCGC	1 cut(s)	3326
GluI	GCNGC	3 cut(s)	310, 1482, 1905
GsaI	CCCAGC	2 cut(s)	2455, 2507
GsuI	CTGGAG	1 cut(s)	3174
HaeIII	GGCC	5 cut(s)	115, 119, 889, 2395, 3011
HapII	CCGG	4 cut(s)	116, 1027, 1061, 2342
HgaI	GACGC	1 cut(s)	3083
HhaI	GCGC	1 cut(s)	3327
Hin6I	GCGC	1 cut(s)	3325
HinP1I	GCGC	1 cut(s)	3325
HindIII	AAGCTT	2 cut(s)	228, 3177
HpaII	CCGG	4 cut(s)	116, 1027, 1061, 2342
HphI	GGTGA	6 cut(s)	50, 172, 1855, 2321, 2836, 3046
Hpy188I	TCNGA	7 cut(s)	417, 624, 1313, 1600, 1780, 2807, 3374
HpyAV	CCTTC	8 cut(s)	42, 80, 696, 2199, 2261, 2660, 2872, 3100
HpyCH4III	ACNGT	7 cut(s)	150, 271, 1567, 2260, 2366, 2614, 2736
HpyCH4IV	ACGT	3 cut(s)	478, 1171, 3244
HpyF10VI	GCNNNNNNNGC	9 cut(s)	236, 283, 1478, 1628, 1955, 2250, 2655, 2671, 3322
HpyF3I	CTNAG	7 cut(s)	171, 1075, 1143, 1310, 1523, 1751, 1767
HpySE526I	ACGT	3 cut(s)	478, 1171, 3244
HspAI	GCGC	1 cut(s)	3325
KflI	GGGWCCC	1 cut(s)	2455
KpnI	GGTACC	1 cut(s)	3193
KroI	GCCGGC	2 cut(s)	115, 1060
KroNI	GCCGGC	2 cut(s)	117, 1062
LguI	GCTCTTC	3 cut(s)	1513, 2119, 2547
LmnI	GCTCC	3 cut(s)	518, 526, 2061
Lsp1109I	GCAGC	3 cut(s)	296, 1468, 1916
LweI	GCATC	7 cut(s)	478, 537, 1293, 2043, 2081, 2926, 3058
MabI	ACCWGGT	1 cut(s)	3119
MaeI	CTAG	8 cut(s)	962, 1272, 1410, 1793, 1802, 3114, 3287, 3338
MaeII	ACGT	3 cut(s)	478, 1171, 3244
MaeIII	GTNAC	6 cut(s)	1561, 1700, 2346, 2533, 3196, 3245
MbiI	CCGCTC	1 cut(s)	3064
MfeI	CAATTG	3 cut(s)	1370, 2538, 2682
MflI	RGATCY	2 cut(s)	790, 922
MhlI	GDGCHC	3 cut(s)	523, 2559, 2667
MlyI	GAGTC	3 cut(s)	164, 668, 1590
MmeI	TCCRAC	4 cut(s)	1751, 2064, 2509, 2964
Mph1103I	ATGCAT	2 cut(s)	279, 2235
MroNI	GCCGGC	2 cut(s)	115, 1060
MroXI	GAANNNNTTC	2 cut(s)	1551, 3148
MseI	TTAA	9 cut(s)	57, 105, 849, 1014, 1149, 1569, 1920, 1943, 2324
MslI	CAYNNNNRTG	2 cut(s)	2307, 2403
MspA1I	CMGCKG	5 cut(s)	239, 1622, 1907, 2717, 2850
MspCI	CTTAAG	1 cut(s)	2323
MspI	CCGG	4 cut(s)	116, 1027, 1061, 2342
MspR9I	CCNGG	3 cut(s)	314, 2307, 3121
MunI	CAATTG	3 cut(s)	1370, 2538, 2682
Mva1269I	GAATGC	1 cut(s)	1355
MvaI	CCWGG	3 cut(s)	314, 2307, 3121
MvnI	CGCG	2 cut(s)	385, 2669
MwoI	GCNNNNNNNGC	9 cut(s)	236, 283, 1478, 1628, 1955, 2250, 2655, 2671, 3322
NaeI	GCCGGC	2 cut(s)	117, 1062
NdeI	CATATG	2 cut(s)	835, 2355
NgoMIV	GCCGGC	2 cut(s)	115, 1060
NlaIV	GGNNCC	5 cut(s)	798, 924, 2456, 2457, 3191
NmeAIII	GCCGAG	1 cut(s)	3301
NmuCI	GTSAC	3 cut(s)	2533, 3196, 3245
NruI	TCGCGA	1 cut(s)	2669
NsiI	ATGCAT	2 cut(s)	279, 2235
NspI	RCATGY	4 cut(s)	305, 552, 1765, 1809
OliI	CACNNNNGTG	1 cut(s)	2307
PagI	TCATGA	1 cut(s)	2527
PaqCI	CACCTGC	1 cut(s)	2164
PceI	AGGCCT	1 cut(s)	2395
PciSI	GCTCTTC	3 cut(s)	1513, 2119, 2547
PcsI	WCGNNNNNNNCGW	1 cut(s)	1168
PctI	GAATGC	1 cut(s)	1355
PdiI	GCCGGC	2 cut(s)	117, 1062
PdmI	GAANNNNTTC	2 cut(s)	1551, 3148
PflMI	CCANNNNNTGG	2 cut(s)	2491, 2705
PkrI	GCNGC	3 cut(s)	311, 1483, 1906
PleI	GAGTC	3 cut(s)	163, 667, 1589
PmaCI	CACGTG	1 cut(s)	479
PmlI	CACGTG	1 cut(s)	479
PpsI	GAGTC	3 cut(s)	163, 667, 1589
Ppu21I	YACGTR	1 cut(s)	479
PpuMI	RGGWCCY	2 cut(s)	2455, 3117
PsiI	TTATAA	2 cut(s)	732, 2022
Psp124BI	GAGCTC	2 cut(s)	523, 2667
Psp5II	RGGWCCY	2 cut(s)	2455, 3117
Psp6I	CCWGG	3 cut(s)	312, 2305, 3119
PspCI	CACGTG	1 cut(s)	479
PspFI	CCCAGC	2 cut(s)	2451, 2503
PspGI	CCWGG	3 cut(s)	312, 2305, 3119
PspN4I	GGNNCC	5 cut(s)	798, 924, 2456, 2457, 3191
PspPI	GGNCC	9 cut(s)	241, 624, 796, 887, 1139, 2094, 2387, 2455, 3117
PspPPI	RGGWCCY	2 cut(s)	2455, 3117
PsrI	GAACNNNNNNTAC	2 cut(s)	1089, 1121
PstI	CTGCAG	1 cut(s)	820
PsuI	RGATCY	2 cut(s)	790, 922
PvuII	CAGCTG	4 cut(s)	1622, 1907, 2717, 2850
RigI	GGCCGGCC	1 cut(s)	119
RruI	TCGCGA	1 cut(s)	2669
RseI	CAYNNNNRTG	2 cut(s)	2307, 2403
SacI	GAGCTC	2 cut(s)	523, 2667
SapI	GCTCTTC	3 cut(s)	1513, 2119, 2547
SaqAI	TTAA	9 cut(s)	57, 105, 849, 1014, 1149, 1569, 1920, 1943, 2324
SatI	GCNGC	3 cut(s)	310, 1482, 1905
Sau96I	GGNCC	9 cut(s)	241, 624, 796, 887, 1139, 2094, 2387, 2455, 3117
SbfI	CCTGCAGG	1 cut(s)	820
SchI	GAGTC	3 cut(s)	164, 668, 1590
ScrFI	CCNGG	3 cut(s)	314, 2307, 3121
SdaI	CCTGCAGG	1 cut(s)	820
SduI	GDGCHC	3 cut(s)	523, 2559, 2667
SexAI	ACCWGGT	1 cut(s)	3119
SfaNI	GCATC	7 cut(s)	478, 537, 1293, 2043, 2081, 2926, 3058
SfcI	CTRYAG	3 cut(s)	816, 2514, 2688
SinI	GGWCC	8 cut(s)	241, 624, 796, 1139, 2094, 2387, 2455, 3117
SmiMI	CAYNNNNRTG	2 cut(s)	2307, 2403
SmlI	CTYRAG	3 cut(s)	1997, 2323, 2791
SmoI	CTYRAG	3 cut(s)	1997, 2323, 2791
Sse8387I	CCTGCAGG	1 cut(s)	820
SseBI	AGGCCT	1 cut(s)	2395
SsiI	CCGC	4 cut(s)	239, 385, 1004, 3064
SspI	AATATT	1 cut(s)	3018
SspMI	CTAG	8 cut(s)	962, 1272, 1410, 1793, 1802, 3114, 3287, 3338
SstI	GAGCTC	2 cut(s)	523, 2667
StuI	AGGCCT	1 cut(s)	2395
StyD4I	CCNGG	3 cut(s)	312, 2305, 3119
StyI	CCWWGG	2 cut(s)	2160, 2390
TaaI	ACNGT	7 cut(s)	150, 271, 1567, 2260, 2366, 2614, 2736
TaiI	ACGT	3 cut(s)	481, 1174, 3247
TaqI	TCGA	3 cut(s)	1191, 1690, 3126
TaqII	GACCGA	1 cut(s)	2786
TatI	WGTACW	3 cut(s)	1909, 1985, 3225
Tru1I	TTAA	9 cut(s)	57, 105, 849, 1014, 1149, 1569, 1920, 1943, 2324
Tru9I	TTAA	9 cut(s)	57, 105, 849, 1014, 1149, 1569, 1920, 1943, 2324
TscAI	CASTG	6 cut(s)	369, 493, 634, 1189, 1755, 2617
TseFI	GTSAC	3 cut(s)	2533, 3196, 3245
TseI	GCWGC	3 cut(s)	309, 1481, 1904
Tsp45I	GTSAC	3 cut(s)	2533, 3196, 3245
TspGWI	ACGGA	2 cut(s)	1114, 2643
TspRI	CASTG	6 cut(s)	369, 493, 634, 1189, 1755, 2617
Van91I	CCANNNNNTGG	2 cut(s)	2491, 2705
Vha464I	CTTAAG	1 cut(s)	2323
VpaK11BI	GGWCC	8 cut(s)	241, 624, 796, 1139, 2094, 2387, 2455, 3117
XapI	RAATTY	6 cut(s)	289, 389, 718, 1340, 1381, 1686
XbaI	TCTAGA	1 cut(s)	3337
XceI	RCATGY	4 cut(s)	305, 552, 1765, 1809
XmiI	GTMKAC	3 cut(s)	1859, 2691, 3168
XmnI	GAANNNNTTC	2 cut(s)	1551, 3148
XspI	CTAG	8 cut(s)	962, 1272, 1410, 1793, 1802, 3114, 3287, 3338
Zsp2I	ATGCAT	2 cut(s)	279, 2235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.