Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Reverse (-)

64152025 .. 64156280

Gene Structure 4256 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ41044

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2862 bp

ATGGAGAAGACAATAAAATTACATGGATTCCCTTCAGGTGAATCTGCTGAAAAAATTAAGGTATTTTTGGAGAAACATACAGGAGAAGGAAGTGTTTTTGCTCTTAAATTACGGCCGGCCAAAGGGGGGAGATTCTATGCTTATATACAGTTTGAGTCCACCAGTCAGGCTGAGTTTATCACCTCTTTGGCTAACGCTAGGGCTAGGCTGTGGTATGGGAGATCCTATTTGAACTTTTATGCAGCGGACCATGATATTGTACCAAAGCCAAGAACTGTAATGCATAGCTTGCAAAATTTAGAATTGCATGTTGGCTGCCAGGTCGCAAATGATAAGTTGTCTGTTTTTTGGAGAACGGCTGAAGTTTCAGTGGACTTTGGGACTGGAACGCGGAAATTCCAATTTCGGCTGTTATATCATCAGAAGTTATATAGACTTCAGCTTTCGTATGAGAACATCTGGCAAATTGAGTTGCATCGTCCACGTGGTCGCACTGCAAAATATCTTGTCATTCAGTTATATGGAGCTCCTCGCATCTACCAACAAGAAGAAGCATGTTCTTCTGGAAATGTATATGATGACCCTCTTATGAACTACTTTAGGGATGTCCCTGATGACCAATGGGTTCGGACCACTGATTTCACTCCACGAAGCTGTATTGGGGAGTCATCTTCATTATGTTTAGAGCTTCCTTCTGGTCGCCAACTTCCTGATTTTGAAGAAAATTTTGCTCATTATAAGGAGAGTGATGGGCAATTTATCTTGGAGAATGGCTCAACTTTCTCTTGCAACCGAGATCTGGTCCCTATTGTTGGTTCTCCTGCAGGGGTTGATTTGCCATATGATATCTTGTTTAAAATAAACTCGCTGGTTCAAATGGGTTGTCTGTCAGGGCCGACACTTGATAATAGGTTTTATAGGCTGGTGGATCCACGAAGAATAACTCGTTCCTGTATAGAGTATGCCCTAGATAAATTATCTAATTTGAAAAAGAGCTGTTATGAACCTGCGGTGTGGCTTAATGAGGAGTACCGGAAGTACCTGACAACAAAGAAACCTCCAAAGCCGGCACTTGTCTCCTTAGATTCTGGTTTGGTATATGTACGGAGGGTTCAAATAACACCATCTAAAGTGTATTTTTCTGGTCCTGAGGTTAATGTCTCAAATCGCATACTACGCCAGTATTCCAGTGATATCGATAACTTTCTTCGTGTTTCATTTGTTGATGAGGAGATGGAAAATCTCTATTCAACAGATTTGTCCCCCCGTACATCTACTAGAAAAACTCGCATCTACAACAGAGTTCTTTCTATTCTCAGAAACGGCATAGTAATTGGAGAAAAGAAATTTGAGTTTCTTGCATTCTCATCAAGTCAATTGCAAGAGAATTCATTGTGGATGTTTTCTTCAACCTCTAATGTTAGTGCTGATAAGATAAGAGCAGGGATGGGGGATTTTCGCAAGATAAGAAATGTGGCAAAGTATGCTGCCAGATTGGGTCAATCTTTTGGCTCTTCTACTGAAACTCTAAGTGTTGGCAGGAATGAGATTGAAGTTATTCCTGATGTAACAGTTAATTGGGGTGGAGACAGATATATGTTCTCTGATGGGATTGGGAAAATATCAGCTGATTTTGCAAGGAGAGTAGCCTTCAAATATGGCTATAAAGCGTTCACACCATCTGCATTTCAAATTCGATATGGTGGTTACAAAGGTGTTGTCGCTGTTGATCCAACCTCGTCAATGAAATTATCACTGAGAAAGAGCATGTCTAAGTATGATTCTGAAAACACAAAGCTAGATGTTCTAGCATGTAGCAAGTTTCAGCCTTGTTTTTTGAATCGTCAGGTGATAACTCTTCTGTCTACACTTGGAGTTCAAGATCATGTTTTCTTGAAAAAGCAAAGAGCAGCTGTACAACAACTTAATGCCATATTGACTGACCCATTAAAAGCAGGGGAGGCTCTTGATTTGATGTCTGCGGGTGAGAGTACAAACATCCTCAAGGAACTGCTGATGTGTGGTTATAAACCTGATGTTGAACCATTCCTTTCAATGATGCTCCAAACATTTCGGGCATCAAAGTTGTTGGAATTGAGGACCAAAACAAGAATCTTTATCCCAGATGGAAGAGCAATGATGGGATGTCTAGACGAAACAAGAACCTTGGAATATGGGCAGGTGTTTGTGCAAATCTGTGGAAACAGACGAAGGCAGTTATTTGATGATTCATTCATGCATAGTGGAAGCTCATCAGGCAAAACAGTTATTGAACGAAAGGTGGTTGTTGCAAAGAACCCATGTTTACACCCCGGTGATGTGCGTGTCTTAAGGGCTGTGAATGTGCCGGAGTTGCAACATATGGTGGACTGTGTGGTATTTCCACAAAAAGGACCAAGGCCTCATCCAAATGAATGTTCTGGAAGTGATCTTGATGGAGATATTTACTTTGTTTGCTGGGACCCTGAGTTGATTCCTCGCAAGCAAATCCAACCTATGGATTATACCCCAGCACCAACTATAGAGATGGATCATGATGTCACAATTGAGGAAGTAGAAGAGCACTTTGTGAATTACATACTCAATGATAGTTTGGGGAGAATTGCCAATGCCCACACTGTTTTTGCTGACAGAGAGCATGATAAAGCAATGAGCCGTCCGTGCATAGAGCTCGCGAAGCTACATTCAATTGCTGTAGACTTTCCAAAAACTGGCGTGCCAGCTGTGAGACCAGAGGAACTGTATGTCAAGGAATATCCAGATTTCATGGAGAAGACCGACAAAAGCACCTATCTCTCAAGCAATGTGATCGGACAGCTTTTTCGTGAGGTGAAGAACATTGCACCCCATGACAGCTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

953

Amino Acids

108.32

Weight (kDa)

8.47

Isoelectric Point (pI)

41.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_RdRP1_2	PF26250	4 - 84	8.2e-15	RdRP1/2, RRM domain
PH_RDR2	PF24823	102 - 253	2e-44	RDR2 PH-like domain
RdRP_helical	PF26252	274 - 348	2e-12	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	373 - 944	1.6e-194	RNA dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	738, 2028
AarI	CACCTGC	1 cut(s)	2170
Acc36I	ACCTGC	2 cut(s)	1015, 2170
AccB7I	CCANNNNNTGG	2 cut(s)	2497, 2711
AccI	GTMKAC	2 cut(s)	1865, 2697
AccII	CGCG	2 cut(s)	391, 2675
AciI	CCGC	4 cut(s)	245, 391, 1010, 1982
AclWI	GGATC	5 cut(s)	216, 923, 936, 1724, 2538
AcoI	YGGCCR	2 cut(s)	113, 117
AcsI	RAATTY	6 cut(s)	295, 395, 724, 1346, 1387, 1692
AcuI	CTGAAG	3 cut(s)	18, 381, 422
AcvI	CACGTG	1 cut(s)	485
AdeI	CACNNNGTG	1 cut(s)	2569
AfaI	GTAC	7 cut(s)	261, 1031, 1040, 1104, 1270, 1917, 1993
AfiI	CCNNNNNNNGG	7 cut(s)	122, 126, 799, 812, 2390, 2497, 2711
AflII	CTTAAG	1 cut(s)	2329
AjnI	CCWGG	1 cut(s)	318
AjuI	GAANNNNNNNTTGG	4 cut(s)	294, 326, 2401, 2433
AleI	CACNNNNGTG	1 cut(s)	2313
Alw21I	GWGCWC	3 cut(s)	529, 2565, 2673
Alw26I	GTCTC	4 cut(s)	1081, 1165, 1581, 2722
AlwI	GGATC	5 cut(s)	216, 923, 936, 1724, 2538
AoxI	GGCC	4 cut(s)	113, 117, 893, 2399
ApeKI	GCWGC	4 cut(s)	242, 315, 1487, 1910
ApoI	RAATTY	6 cut(s)	295, 395, 724, 1346, 1387, 1692
ArsI	GACNNNNNNTTYG	6 cut(s)	868, 900, 1704, 1736, 2805, 2837
Asp700I	GAANNNNTTC	1 cut(s)	1557
AspS9I	GGNCC	8 cut(s)	247, 630, 802, 893, 1145, 2100, 2393, 2461
AsuC2I	CCSGG	1 cut(s)	2313
AsuHPI	GGTGA	6 cut(s)	50, 172, 1861, 1997, 2327, 2842
AvaII	GGWCC	7 cut(s)	247, 630, 802, 1145, 2100, 2393, 2461
AxyI	CCTNAGG	1 cut(s)	1149
BamHI	GGATCC	1 cut(s)	928
BanII	GRGCYC	2 cut(s)	529, 2673
BbrPI	CACGTG	1 cut(s)	485
BbsI	GAAGAC	2 cut(s)	14, 2780
Bbv12I	GWGCWC	3 cut(s)	529, 2565, 2673
BbvI	GCAGC	4 cut(s)	254, 302, 1474, 1922
BceAI	ACGGC	4 cut(s)	128, 372, 1339, 2640
BcgI	CGANNNNNNTGC	4 cut(s)	2653, 2687, 2791, 2825
BciT130I	CCWGG	1 cut(s)	320
BcnI	CCSGG	1 cut(s)	2313
BcoDI	GTCTC	4 cut(s)	1081, 1165, 1581, 2722
BfaI	CTAG	7 cut(s)	198, 204, 968, 1278, 1799, 1808, 2150
BfmI	CTRYAG	3 cut(s)	822, 2520, 2694
BfrI	CTTAAG	1 cut(s)	2329
BfuAI	ACCTGC	2 cut(s)	1015, 2170
BglII	AGATCT	1 cut(s)	796
BisI	GCNGC	4 cut(s)	243, 316, 1488, 1911
BlsI	GCNGC	4 cut(s)	244, 317, 1489, 1912
Bme1390I	CCNGG	2 cut(s)	320, 2313
Bme18I	GGWCC	7 cut(s)	247, 630, 802, 1145, 2100, 2393, 2461
BmgT120I	GGNCC	8 cut(s)	247, 630, 802, 893, 1145, 2100, 2393, 2461
BmiI	GGNNCC	4 cut(s)	804, 930, 2462, 2463
BmrFI	CCNGG	2 cut(s)	320, 2313
BmsI	GCATC	5 cut(s)	484, 543, 1299, 2049, 2087
BpiI	GAAGAC	2 cut(s)	14, 2780
BplI	GAGNNNNNCTC	2 cut(s)	758, 790
BpuEI	CTTGAG	2 cut(s)	1988, 2782
BpuMI	CCSGG	1 cut(s)	2313
Bsa29I	ATCGAT	1 cut(s)	1197
BsaAI	YACGTR	1 cut(s)	485
BsaI	GGTCTC	1 cut(s)	2722
BsaJI	CCNNGG	3 cut(s)	2166, 2311, 2396
BsaWI	WCCGGW	1 cut(s)	1032
Bsc4I	CCNNNNNNNGG	7 cut(s)	122, 126, 799, 812, 2390, 2497, 2711
Bse118I	RCCGGY	2 cut(s)	115, 1066
Bse1I	ACTGG	5 cut(s)	162, 388, 1180, 1188, 2716
Bse21I	CCTNAGG	1 cut(s)	1149
Bse3DI	GCAATG	4 cut(s)	2142, 2655, 2809, 2838
BseBI	CCWGG	1 cut(s)	320
BseCI	ATCGAT	1 cut(s)	1197
BseDI	CCNNGG	3 cut(s)	2166, 2311, 2396
BseGI	GGATG	6 cut(s)	610, 1404, 1452, 1998, 2150, 2404
BseLI	CCNNNNNNNGG	7 cut(s)	122, 126, 799, 812, 2390, 2497, 2711
BseMI	GCAATG	4 cut(s)	2142, 2655, 2809, 2838
BseMII	CTCAG	5 cut(s)	162, 1140, 1330, 1748, 2457
BseNI	ACTGG	5 cut(s)	162, 388, 1180, 1188, 2716
BseRI	GAGGAG	3 cut(s)	519, 1040, 1244
BseX3I	CGGCCG	1 cut(s)	113
BseXI	GCAGC	4 cut(s)	254, 302, 1474, 1922
BseYI	CCCAGC	2 cut(s)	2457, 2509
Bsh1236I	CGCG	2 cut(s)	391, 2675
Bsh1285I	CGRYCG	1 cut(s)	116
BshFI	GGCC	4 cut(s)	115, 119, 895, 2401
BshVI	ATCGAT	1 cut(s)	1197
BsiEI	CGRYCG	1 cut(s)	116
BsiHKAI	GWGCWC	3 cut(s)	529, 2565, 2673
BsiSI	CCGG	5 cut(s)	116, 1033, 1067, 2313, 2348
BslFI	GGGAC	5 cut(s)	394, 593, 788, 1246, 2474
BslI	CCNNNNNNNGG	7 cut(s)	122, 126, 799, 812, 2390, 2497, 2711
BsmAI	GTCTC	4 cut(s)	1081, 1165, 1581, 2722
BsmFI	GGGAC	5 cut(s)	394, 593, 788, 1246, 2474
BsmI	GAATGC	1 cut(s)	1361
BsnI	GGCC	4 cut(s)	115, 119, 895, 2401
Bso31I	GGTCTC	1 cut(s)	2722
Bsp1286I	GDGCHC	3 cut(s)	529, 2565, 2673
Bsp1407I	TGTACA	1 cut(s)	1915
Bsp143I	GATC	8 cut(s)	221, 796, 928, 1729, 1882, 2428, 2530, 2808
Bsp68I	TCGCGA	1 cut(s)	2675
BspACI	CCGC	4 cut(s)	245, 391, 1010, 1982
BspANI	GGCC	4 cut(s)	115, 119, 895, 2401
BspCNI	CTCAG	5 cut(s)	163, 1141, 1329, 1749, 2458
BspDI	ATCGAT	1 cut(s)	1197
BspFNI	CGCG	2 cut(s)	391, 2675
BspHI	TCATGA	1 cut(s)	2533
BspLI	GGNNCC	4 cut(s)	804, 930, 2462, 2463
BspMAI	CTGCAG	1 cut(s)	826
BspMI	ACCTGC	2 cut(s)	1015, 2170
BspPI	GGATC	5 cut(s)	216, 923, 936, 1724, 2538
BspQI	GCTCTTC	3 cut(s)	1519, 2125, 2553
BspTI	CTTAAG	1 cut(s)	2329
BspTNI	GGTCTC	1 cut(s)	2722
BsrDI	GCAATG	4 cut(s)	2142, 2655, 2809, 2838
BsrFI	RCCGGY	2 cut(s)	115, 1066
BsrGI	TGTACA	1 cut(s)	1915
BsrI	ACTGG	5 cut(s)	162, 388, 1180, 1188, 2716
BssAI	RCCGGY	2 cut(s)	115, 1066
BssECI	CCNNGG	3 cut(s)	2166, 2311, 2396
BssMI	GATC	8 cut(s)	221, 796, 928, 1729, 1882, 2428, 2530, 2808
BssT1I	CCWWGG	2 cut(s)	2166, 2396
Bst2UI	CCWGG	1 cut(s)	320
Bst4CI	ACNGT	7 cut(s)	150, 277, 1573, 2266, 2372, 2620, 2742
Bst6I	CTCTTC	4 cut(s)	1519, 1863, 2125, 2553
BstAFI	CTTAAG	1 cut(s)	2329
BstAPI	GCANNNNNTGC	2 cut(s)	289, 1484
BstAUI	TGTACA	1 cut(s)	1915
BstBAI	YACGTR	1 cut(s)	485
BstC8I	GCNNGC	7 cut(s)	117, 290, 1068, 2483, 2673, 2717, 2721
BstDEI	CTNAG	8 cut(s)	171, 1081, 1149, 1316, 1529, 1757, 1773, 2466
BstF5I	GGATG	6 cut(s)	610, 1404, 1452, 1998, 2150, 2404
BstFNI	CGCG	2 cut(s)	391, 2675
BstKTI	GATC	8 cut(s)	224, 799, 931, 1732, 1885, 2431, 2533, 2811
BstMAI	GTCTC	4 cut(s)	1081, 1165, 1581, 2722
BstMBI	GATC	8 cut(s)	221, 796, 928, 1729, 1882, 2428, 2530, 2808
BstMCI	CGRYCG	1 cut(s)	116
BstMWI	GCNNNNNNNGC	9 cut(s)	289, 1176, 1484, 1634, 1961, 2256, 2353, 2661, 2677
BstNI	CCWGG	1 cut(s)	320
BstNSI	RCATGY	4 cut(s)	311, 558, 1771, 1815
BstSCI	CCNGG	2 cut(s)	318, 2311
BstSFI	CTRYAG	3 cut(s)	822, 2520, 2694
BstUI	CGCG	2 cut(s)	391, 2675
BstV1I	GCAGC	4 cut(s)	254, 302, 1474, 1922
BstV2I	GAAGAC	2 cut(s)	14, 2780
BstX2I	RGATCY	3 cut(s)	221, 796, 928
BstYI	RGATCY	3 cut(s)	221, 796, 928
BstZI	CGGCCG	1 cut(s)	113
Bsu15I	ATCGAT	1 cut(s)	1197
Bsu36I	CCTNAGG	1 cut(s)	1149
BsuRI	GGCC	4 cut(s)	115, 119, 895, 2401
BsuTUI	ATCGAT	1 cut(s)	1197
BtsCI	GGATG	6 cut(s)	610, 1404, 1452, 1998, 2150, 2404
BtsI	GCAGTG	1 cut(s)	492
BtsIMutI	CAGTG	6 cut(s)	375, 492, 633, 1195, 1754, 2616
BtuMI	TCGCGA	1 cut(s)	2675
BveI	ACCTGC	2 cut(s)	1015, 2170
Cac8I	GCNNGC	7 cut(s)	117, 290, 1068, 2483, 2673, 2717, 2721
CciI	TCATGA	1 cut(s)	2533
Cfr10I	RCCGGY	2 cut(s)	115, 1066
Cfr13I	GGNCC	8 cut(s)	247, 630, 802, 893, 1145, 2100, 2393, 2461
ClaI	ATCGAT	1 cut(s)	1197
Csp6I	GTAC	7 cut(s)	260, 1030, 1039, 1103, 1269, 1916, 1992
CviQI	GTAC	7 cut(s)	260, 1030, 1039, 1103, 1269, 1916, 1992
DdeI	CTNAG	8 cut(s)	171, 1081, 1149, 1316, 1529, 1757, 1773, 2466
DpnI	GATC	8 cut(s)	223, 798, 930, 1731, 1884, 2430, 2532, 2810
DpnII	GATC	8 cut(s)	221, 796, 928, 1729, 1882, 2428, 2530, 2808
DraI	TTTAAA	1 cut(s)	856
DraIII	CACNNNGTG	1 cut(s)	2569
EaeI	YGGCCR	2 cut(s)	113, 117
EagI	CGGCCG	1 cut(s)	113
Eam1104I	CTCTTC	4 cut(s)	1519, 1863, 2125, 2553
EarI	CTCTTC	4 cut(s)	1519, 1863, 2125, 2553
Ecl136II	GAGCTC	2 cut(s)	527, 2671
EclXI	CGGCCG	1 cut(s)	113
Eco130I	CCWWGG	2 cut(s)	2166, 2396
Eco147I	AGGCCT	1 cut(s)	2401
Eco24I	GRGCYC	2 cut(s)	529, 2673
Eco31I	GGTCTC	1 cut(s)	2722
Eco32I	GATATC	2 cut(s)	847, 1195
Eco47I	GGWCC	7 cut(s)	247, 630, 802, 1145, 2100, 2393, 2461
Eco52I	CGGCCG	1 cut(s)	113
Eco53kI	GAGCTC	2 cut(s)	527, 2671
Eco57I	CTGAAG	3 cut(s)	18, 381, 422
Eco72I	CACGTG	1 cut(s)	485
Eco81I	CCTNAGG	1 cut(s)	1149
EcoICRI	GAGCTC	2 cut(s)	527, 2671
EcoO109I	RGGNCCY	1 cut(s)	2461
EcoRI	GAATTC	1 cut(s)	1387
EcoRII	CCWGG	1 cut(s)	318
EcoRV	GATATC	2 cut(s)	847, 1195
EcoT14I	CCWWGG	2 cut(s)	2166, 2396
EcoT22I	ATGCAT	2 cut(s)	285, 2241
EcoT38I	GRGCYC	2 cut(s)	529, 2673
ErhI	CCWWGG	2 cut(s)	2166, 2396
FaqI	GGGAC	5 cut(s)	394, 593, 788, 1246, 2474
FauI	CCCGC	1 cut(s)	1975
FauNDI	CATATG	2 cut(s)	841, 2361
FblI	GTMKAC	2 cut(s)	1865, 2697
Fnu4HI	GCNGC	4 cut(s)	243, 316, 1488, 1911
FokI	GGATG	6 cut(s)	617, 1411, 1459, 1985, 2157, 2391
FriOI	GRGCYC	2 cut(s)	529, 2673
FseI	GGCCGGCC	1 cut(s)	119
Fsp4HI	GCNGC	4 cut(s)	243, 316, 1488, 1911
FspBI	CTAG	7 cut(s)	198, 204, 968, 1278, 1799, 1808, 2150
GluI	GCNGC	4 cut(s)	243, 316, 1488, 1911
GsaI	CCCAGC	2 cut(s)	2461, 2513
HaeIII	GGCC	4 cut(s)	115, 119, 895, 2401
HapII	CCGG	5 cut(s)	116, 1033, 1067, 2313, 2348
HpaII	CCGG	5 cut(s)	116, 1033, 1067, 2313, 2348
HphI	GGTGA	6 cut(s)	50, 172, 1861, 1997, 2327, 2842
Hpy166II	GTNNAC	8 cut(s)	159, 373, 482, 1674, 1866, 2306, 2368, 2698
Hpy188I	TCNGA	6 cut(s)	423, 630, 1319, 1606, 1786, 2813
Hpy8I	GTNNAC	8 cut(s)	159, 373, 482, 1674, 1866, 2306, 2368, 2698
HpyAV	CCTTC	5 cut(s)	42, 80, 702, 1660, 2205
HpyCH4III	ACNGT	7 cut(s)	150, 277, 1573, 2266, 2372, 2620, 2742
HpyCH4IV	ACGT	1 cut(s)	484
HpyF10VI	GCNNNNNNNGC	9 cut(s)	289, 1176, 1484, 1634, 1961, 2256, 2353, 2661, 2677
HpyF3I	CTNAG	8 cut(s)	171, 1081, 1149, 1316, 1529, 1757, 1773, 2466
HpySE526I	ACGT	1 cut(s)	484
KflI	GGGWCCC	1 cut(s)	2461
KroI	GCCGGC	2 cut(s)	115, 1066
KroNI	GCCGGC	2 cut(s)	117, 1068
Kzo9I	GATC	8 cut(s)	221, 796, 928, 1729, 1882, 2428, 2530, 2808
LguI	GCTCTTC	3 cut(s)	1519, 2125, 2553
LmnI	GCTCC	3 cut(s)	524, 532, 2067
Lsp1109I	GCAGC	4 cut(s)	254, 302, 1474, 1922
LweI	GCATC	5 cut(s)	484, 543, 1299, 2049, 2087
MaeI	CTAG	7 cut(s)	198, 204, 968, 1278, 1799, 1808, 2150
MaeII	ACGT	1 cut(s)	484
MaeIII	GTNAC	3 cut(s)	1567, 1706, 2539
MalI	GATC	8 cut(s)	223, 798, 930, 1731, 1884, 2430, 2532, 2810
MboI	GATC	8 cut(s)	221, 796, 928, 1729, 1882, 2428, 2530, 2808
MfeI	CAATTG	3 cut(s)	1376, 2544, 2688
MflI	RGATCY	3 cut(s)	221, 796, 928
MhlI	GDGCHC	3 cut(s)	529, 2565, 2673
MlyI	GAGTC	2 cut(s)	164, 674
MmeI	TCCRAC	3 cut(s)	1757, 2070, 2515
Mph1103I	ATGCAT	2 cut(s)	285, 2241
MroNI	GCCGGC	2 cut(s)	115, 1066
MroXI	GAANNNNTTC	1 cut(s)	1557
MseI	TTAA	9 cut(s)	57, 105, 855, 1020, 1155, 1575, 1926, 1949, 2330
MslI	CAYNNNNRTG	2 cut(s)	2313, 2409
MspA1I	CMGCKG	5 cut(s)	245, 1628, 1913, 2723, 2856
MspCI	CTTAAG	1 cut(s)	2329
MspI	CCGG	5 cut(s)	116, 1033, 1067, 2313, 2348
MspR9I	CCNGG	2 cut(s)	320, 2313
MunI	CAATTG	3 cut(s)	1376, 2544, 2688
Mva1269I	GAATGC	1 cut(s)	1361
MvaI	CCWGG	1 cut(s)	320
MvnI	CGCG	2 cut(s)	391, 2675
MwoI	GCNNNNNNNGC	9 cut(s)	289, 1176, 1484, 1634, 1961, 2256, 2353, 2661, 2677
NaeI	GCCGGC	2 cut(s)	117, 1068
NciI	CCSGG	1 cut(s)	2313
NdeI	CATATG	2 cut(s)	841, 2361
NdeII	GATC	8 cut(s)	221, 796, 928, 1729, 1882, 2428, 2530, 2808
NgoMIV	GCCGGC	2 cut(s)	115, 1066
NlaIV	GGNNCC	4 cut(s)	804, 930, 2462, 2463
NmuCI	GTSAC	1 cut(s)	2539
NruI	TCGCGA	1 cut(s)	2675
NsiI	ATGCAT	2 cut(s)	285, 2241
NspI	RCATGY	4 cut(s)	311, 558, 1771, 1815
OliI	CACNNNNGTG	1 cut(s)	2313
PagI	TCATGA	1 cut(s)	2533
PaqCI	CACCTGC	1 cut(s)	2170
PceI	AGGCCT	1 cut(s)	2401
PciSI	GCTCTTC	3 cut(s)	1519, 2125, 2553
PctI	GAATGC	1 cut(s)	1361
PdiI	GCCGGC	2 cut(s)	117, 1068
PdmI	GAANNNNTTC	1 cut(s)	1557
PfeI	GAWTC	9 cut(s)	27, 41, 132, 1085, 1781, 1840, 2112, 2228, 2473
PflMI	CCANNNNNTGG	2 cut(s)	2497, 2711
PkrI	GCNGC	4 cut(s)	244, 317, 1489, 1912
PleI	GAGTC	2 cut(s)	163, 673
PmaCI	CACGTG	1 cut(s)	485
PmlI	CACGTG	1 cut(s)	485
PpsI	GAGTC	2 cut(s)	163, 673
Ppu21I	YACGTR	1 cut(s)	485
PpuMI	RGGWCCY	1 cut(s)	2461
PsiI	TTATAA	2 cut(s)	738, 2028
Psp124BI	GAGCTC	2 cut(s)	529, 2673
Psp5II	RGGWCCY	1 cut(s)	2461
Psp6I	CCWGG	1 cut(s)	318
PspCI	CACGTG	1 cut(s)	485
PspFI	CCCAGC	2 cut(s)	2457, 2509
PspGI	CCWGG	1 cut(s)	318
PspN4I	GGNNCC	4 cut(s)	804, 930, 2462, 2463
PspPI	GGNCC	8 cut(s)	247, 630, 802, 893, 1145, 2100, 2393, 2461
PspPPI	RGGWCCY	1 cut(s)	2461
PsrI	GAACNNNNNNTAC	2 cut(s)	1095, 1127
PstI	CTGCAG	1 cut(s)	826
PsuI	RGATCY	3 cut(s)	221, 796, 928
PvuII	CAGCTG	4 cut(s)	1628, 1913, 2723, 2856
RigI	GGCCGGCC	1 cut(s)	119
RruI	TCGCGA	1 cut(s)	2675
RsaI	GTAC	7 cut(s)	261, 1031, 1040, 1104, 1270, 1917, 1993
RsaNI	GTAC	7 cut(s)	260, 1030, 1039, 1103, 1269, 1916, 1992
RseI	CAYNNNNRTG	2 cut(s)	2313, 2409
SacI	GAGCTC	2 cut(s)	529, 2673
SapI	GCTCTTC	3 cut(s)	1519, 2125, 2553
SaqAI	TTAA	9 cut(s)	57, 105, 855, 1020, 1155, 1575, 1926, 1949, 2330
SatI	GCNGC	4 cut(s)	243, 316, 1488, 1911
Sau3AI	GATC	8 cut(s)	221, 796, 928, 1729, 1882, 2428, 2530, 2808
Sau96I	GGNCC	8 cut(s)	247, 630, 802, 893, 1145, 2100, 2393, 2461
SbfI	CCTGCAGG	1 cut(s)	826
SchI	GAGTC	2 cut(s)	164, 674
ScrFI	CCNGG	2 cut(s)	320, 2313
SdaI	CCTGCAGG	1 cut(s)	826
SduI	GDGCHC	3 cut(s)	529, 2565, 2673
SfaNI	GCATC	5 cut(s)	484, 543, 1299, 2049, 2087
SfcI	CTRYAG	3 cut(s)	822, 2520, 2694
SinI	GGWCC	7 cut(s)	247, 630, 802, 1145, 2100, 2393, 2461
SmiMI	CAYNNNNRTG	2 cut(s)	2313, 2409
SmlI	CTYRAG	3 cut(s)	2003, 2329, 2797
SmoI	CTYRAG	3 cut(s)	2003, 2329, 2797
Sse8387I	CCTGCAGG	1 cut(s)	826
SseBI	AGGCCT	1 cut(s)	2401
SsiI	CCGC	4 cut(s)	245, 391, 1010, 1982
SspMI	CTAG	7 cut(s)	198, 204, 968, 1278, 1799, 1808, 2150
SstI	GAGCTC	2 cut(s)	529, 2673
StuI	AGGCCT	1 cut(s)	2401
StyD4I	CCNGG	2 cut(s)	318, 2311
StyI	CCWWGG	2 cut(s)	2166, 2396
TaaI	ACNGT	7 cut(s)	150, 277, 1573, 2266, 2372, 2620, 2742
TaiI	ACGT	1 cut(s)	487
TaqI	TCGA	2 cut(s)	1197, 1696
TaqII	GACCGA	1 cut(s)	2792
TatI	WGTACW	2 cut(s)	1915, 1991
TfiI	GAWTC	9 cut(s)	27, 41, 132, 1085, 1781, 1840, 2112, 2228, 2473
Tru1I	TTAA	9 cut(s)	57, 105, 855, 1020, 1155, 1575, 1926, 1949, 2330
Tru9I	TTAA	9 cut(s)	57, 105, 855, 1020, 1155, 1575, 1926, 1949, 2330
TscAI	CASTG	6 cut(s)	375, 499, 640, 1195, 1761, 2623
TseFI	GTSAC	1 cut(s)	2539
TseI	GCWGC	4 cut(s)	242, 315, 1487, 1910
Tsp45I	GTSAC	1 cut(s)	2539
TspGWI	ACGGA	2 cut(s)	1120, 2649
TspRI	CASTG	6 cut(s)	375, 499, 640, 1195, 1761, 2623
Van91I	CCANNNNNTGG	2 cut(s)	2497, 2711
Vha464I	CTTAAG	1 cut(s)	2329
VpaK11BI	GGWCC	7 cut(s)	247, 630, 802, 1145, 2100, 2393, 2461
XapI	RAATTY	6 cut(s)	295, 395, 724, 1346, 1387, 1692
XbaI	TCTAGA	1 cut(s)	2149
XceI	RCATGY	4 cut(s)	311, 558, 1771, 1815
XmiI	GTMKAC	2 cut(s)	1865, 2697
XmnI	GAANNNNTTC	1 cut(s)	1557
XspI	CTAG	7 cut(s)	198, 204, 968, 1278, 1799, 1808, 2150
Zsp2I	ATGCAT	2 cut(s)	285, 2241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.