Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Forward (+)

51478436 .. 51483210

Gene Structure 4775 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0342200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3792 bp

ATGGGGAAGAAAGGTGGGTTGTTCAGGTATGCAGATGGTGTTGACAAGTTACTGATGGTGTTTGGAACTTTGGGTAGCATTGGAGATGGGTTGATGACACCTCTTACAATGTTTGTGCTCAGCCGCGTCATCAATGAGTATGGAGCTGCAACTCTTACTTTCTCTAACGACATTGTCGACGAGTACTCACTCAAGCTGCTCAGTGTAGCAATTGGAGTTGGAATATCTGCTTTCATTGAAGGGTTTTGTTGGACCAGAACTGCTGAGAGACAGACATCCAGAATGAGAATGGAATACTTAAAATCAGTCCTTAGGCAAGACGTTGCGTTCTTTGACAGTCAAGCAAATTCTTCCATGACCTTCCAAGTCATTTCCACAATCTCTTCTGATGCTCATTTTATCCAAGACACAATAGCTGAGAAGCTACCAAATTGCCTGGCTCACCTCTCATCATTTCTGTTCTGCTTTCCTGTTGCCTTTGTGCTTTCTTGGAGATTGGCACTGGCTGCTCTTCCATTCTCGGTCATGTTTATCATCCCCGGATTGGGATTTGGGAAGGTAATGCAAGACTTAGGAGTTAAGATAAATGGTGCATATGGAGTTGCTGGAGGGATTGCAGAACAAGCAATCTCTTCAATCCGCACAGTCTATTCTTATGTTGGGGAGCGTCAAACACTGAAGAAGTTCAGCATTGCTCTTGAGAAAGTTACACAGCTTGGCATAAAGCAAGGATTCACAAAGGGATTACTAATAGGGAGCATGGGAATGATCTATGCTGCTTGGGCTTTTCAGGCTTGGGTTGGAAGCAAACTTGTTATTGAGAGAGGTGAAAAGGGTGGCCTCGTTTTTGTTTCTGGAATCTGTGTAATTCTGGGAGGATTGGCCATTATGAATGCACTTCCAAATCTCTCCTTCATCACTCAGGCAAGTACTGCTGCTACCCGAATATCTGAGATGATTGATCGCGTTCCGGTTATTGACTCTGAAGATGAAAGGGGAAAAGTTTTACCATATGTGAAAGGAGATATTGAGTTCAGAAAGGTTGACTTCTTCTACCCGTCAAGACCTGATACCCCAATTCTTCAAGGACTCAGCCTTAAGGTTCAAGCTGGTAAGATGCTGGGTCTTGTTGGAAGCAGTGGCTCTGGAAAGTCAACAATCATTTCTTTGCTTGAAAGATTTTATGATCCAGTAAAAGGGGAAATTCTCCTTGATGGACACAAATTAAAGAAGTTTCAGCTGCACTGGTTGAGGTCCCAGATGGGGCTGGTTAATCAAGAACCGATTCTCTTTGCAACATCCATAATAGAGAATATTTTATTTGGGAAGGAAGGAGCTTCTATGGAAGATGTGACAAGTGCAGCTAAGGCTGCAAATGCACATGACTTCATTGTTAAACTACCTGCAGGATATCACACTCAAGTTGGGCAATTTGGAGTTCAATTGTCTGGAGGGCAAAAGCAAAGGATTTCCATAGCAAGAGCAATAATCAGAGACCCGAAAATTCTTTTGCTTGATGAAGCAACAAGTGCTTTGGATGCTCAGTCTGAAACTATTGTACAAGAAGCCTTAGACCAAGCATCGCAAGGAAGAACAACCATTGTCATAGCTCACCGCCTCAGCACAATACGTAAGGCTGATACAATAGTGGTTCTTCAATCAGGGAGAGTGGTTGAGAAGGGATCCCATGACGAGTTGGTCAACATGAGAAATGGAGAAGCTGGAGCTTACAAAAAAATGCTGCAATTACATAAACAAGCCATGCAAAATAGTAACCCCAACAGAATGGAGCAACCTCAAACTCCACAGACAGCAAGGCGTTTGGGGTCAAGCTGGCAAAATAGCCCAGGACCACTAAGTGGGAGGTCAAGTTGGCACACAACAAAGAGTGTTCACTCAAGCTGGCAAAACAGCCCACTGACCCCAGCATATGCAGTCACCCCAATATTTTCCATTAGTATAGCGAACTCCATCCAAACTCCCCAGTATGATGACTTTGACAATGAATGGTCAAAAGAAATCTCTAAAACTTCCTCATCTCAGTGGCGTTTGTTTCAAATGAATGCACCAGAGTGGAAGCAAGCAACACTGGGGTGTCTAGGTGCTGCTGGCTTTGGAACTCTTCAGCCAATTCATGCCTACTGCTTAGGAACAGTAGTGTCAATTTACTATGGATCAGACGATTCCACCATCAGATCACAGACCAGATTCTACTGCTACATCTTCTTAGGCTTAGCAGTTTTCAGCTTCTTTGCTAATCTCCTCCAGCATTACAACTTTGCAGTCATGGGAGAGAGATTATCTGAAAGAGTTCGAACAAAATTGCTAGAAAAACTTCTTACCTTTGAGATAGGGTGGTTTGATCAAGATGAGAACACAAGTGCTGCCATCTGTGCCCGGCTAACCACTGAAGCCAACATTGTTAGATCCCTTACTGCAGACCGTATCTCATTGTTGGTTCAGGTTTTCTTCAGTGCCACCATAGCTTATATACTCGGATTAATGGTCACATGGAGGATAGCCATTGTCATGATTGCAATACAGCCTTTGCTCATTGGAAGCTTCTATTATAGGAGTGTTCTAATGAAGAGTATGTCGAGAAAAGCACAGAAATCACAGGCTGTAGGTAGTCAACTTGCAAGTGAAGCTACTTTCAACCACAGGACAATCACTGCATTTTCCTCACAGACAAGGATATTAAAACTCTTTCGAGATGCTATGCAAGGCCCGAGGAACGAGAACATCAAACAGTCATGGGTTGCGGGTTTTGGTTTGTTCAGCTCTCAATTTCTGACCACAGCTGCTATAGCTTTGACATATTGGTATGGAGGGAGGCTTATGAATCAGAACTTAATATCATCAACGCACCTATTTCAAGTTTTCTTCATCCTAATGAGCACTGGTAAGAACATTGCAGATGCAGGAAGCATGACTTCTGATCTTGCAAGAAGTGGGAAAGCCATCACCTCAATATTTGCTATTCTAGACAGAGAAAGTGAGATTTCTTCTGAGGAGCTTAAAGGGGCTAAAAAGACTTTCAAGGGTTACATAGAGCTGAAAAATGTGGTATTCTCATACCCAGTCAGGCCTGACCAGATGATATTTAAAGGCTTGAATCTCAAGATTAAAGCTGGAAACACAATGGCACTAGTGGGACAGAGTGGTTCAGGCAAATCCACTGTCATTGGCTTGATTGAAAGATTTTACGATCCACTAAATGGATCCGTATTCATAGATGGATGTGACATCAAGCAGTATAACTTGAAGAACTTGAGGTCACAGATTGCTTTGGTAAGCCAAGAGCCTACTCTATTTGGTGGCACAATCCAAGAAAACATTGTATATGGTAAAGAAAATGCAACAGTGGCTGAAGTAAGGAAGGCTGCAAAACAAGCCAATGCTCATGAGTTTATAAGCTCCATGGAAAATGGATATGACACTTATTGTGGAGAAAGAGGAGTTCAGCTATCAGGTGGCCAGAAGCAGAGAATAGCACTTGCCCGAGCCATACTAAAGAACCCAAGTATCCTTCTCTTGGATGAAGCAACCAGTGCCCTTGATAGCGTGTCAGAGAATCTAGTCCAAGAAGCACTGGAGAAGATGATGGTTGGCAGAACATGTGTAGTGGTAGCTCACAGGTTGTCCACAATACAAAAATCAGACTCCATAGCGGTGATAATGAATGGGAAAGTAGCAGAACAAGGATCACACTCTGAACTGGTTTCAATTGGAAGTGGTGGTGCCTACTACTCTTTGATAAAACTACAAAGCAATCAAGCAATCTCAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

1263

Amino Acids

138.48

Weight (kDa)

9.2

Isoelectric Point (pI)

34.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	20 - 278	1.7e-42	ABC transporter transmembrane region
ABC_tran	PF00005	361 - 510	2.4e-32	ABC transporter
SMC_N	PF02463	401 - 552	2.1e-06	RecF/RecN/SMC N terminal domain
ABC_membrane	PF00664	702 - 966	5.7e-45	ABC transporter transmembrane region
ABC_tran	PF00005	1036 - 1184	3.8e-33	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3413
AasI	GACNNNNNNGTC	1 cut(s)	1697
Acc36I	ACCTGC	1 cut(s)	1411
AccB1I	GGYRCC	1 cut(s)	3740
AccB7I	CCANNNNNTGG	2 cut(s)	1859, 3218
AccI	GTMKAC	1 cut(s)	177
AccII	CGCG	2 cut(s)	126, 966
AciI	CCGC	5 cut(s)	124, 640, 1615, 2762, 3671
AclWI	GGATC	9 cut(s)	1181, 1677, 1690, 2182, 2421, 3203, 3216, 3229, 3712
AcoI	YGGCCR	2 cut(s)	882, 3475
AcsI	RAATTY	3 cut(s)	346, 1203, 1503
AcuI	CTGAAG	6 cut(s)	698, 1005, 2108, 2430, 2455, 3390
AdeI	CACNNNGTG	1 cut(s)	1859
AfaI	GTAC	3 cut(s)	185, 931, 1560
AfiI	CCNNNNNNNGG	7 cut(s)	544, 545, 1196, 1263, 1859, 3218, 3535
AflII	CTTAAG	1 cut(s)	1097
AflIII	ACRYGT	1 cut(s)	3617
AhlI	ACTAGT	1 cut(s)	3148
AjnI	CCWGG	2 cut(s)	435, 1846
AleI	CACNNNNGTG	2 cut(s)	2071, 2092
Alw21I	GWGCWC	2 cut(s)	120, 2900
Alw26I	GTCTC	2 cut(s)	262, 1488
AlwI	GGATC	9 cut(s)	1181, 1677, 1690, 2182, 2421, 3203, 3216, 3229, 3712
AlwNI	CAGNNNCTG	1 cut(s)	3368
Ama87I	CYCGRG	2 cut(s)	2728, 3501
AoxI	GGCC	5 cut(s)	838, 882, 2725, 3086, 3475
ApoI	RAATTY	3 cut(s)	346, 1203, 1503
ArsI	GACNNNNNNTTYG	2 cut(s)	3165, 3197
AseI	ATTAAT	1 cut(s)	2501
Asp700I	GAANNNNTTC	4 cut(s)	683, 1284, 2310, 2334
AspS9I	GGNCC	4 cut(s)	252, 1254, 1850, 2726
AsuC2I	CCSGG	2 cut(s)	540, 2398
AsuHPI	GGTGA	6 cut(s)	434, 839, 1604, 1930, 2956, 3685
AsuII	TTCGAA	1 cut(s)	2314
AvaI	CYCGRG	2 cut(s)	2728, 3501
AvaII	GGWCC	3 cut(s)	252, 1254, 1850
AxyI	CCTNAGG	1 cut(s)	311
BaeGI	GKGCMC	2 cut(s)	2398, 3556
BaeI	ACNNNNGTAYC	4 cut(s)	1632, 1665, 3508, 3541
BalI	TGGCCA	2 cut(s)	884, 3477
BamHI	GGATCC	2 cut(s)	1682, 3221
BanI	GGYRCC	1 cut(s)	3740
Bbv12I	GWGCWC	2 cut(s)	120, 2900
BbvCI	CCTCAGC	1 cut(s)	1619
BciT130I	CCWGG	2 cut(s)	437, 1848
BciVI	GTATCC	1 cut(s)	3536
BclI	TGATCA	1 cut(s)	2362
BcnI	CCSGG	2 cut(s)	540, 2398
BcoDI	GTCTC	2 cut(s)	262, 1488
BcuI	ACTAGT	1 cut(s)	3148
BfaI	CTAG	5 cut(s)	2099, 2327, 2984, 3149, 3578
BfmI	CTRYAG	4 cut(s)	1404, 2436, 2622, 2805
BfrI	CTTAAG	1 cut(s)	1097
BfuAI	ACCTGC	1 cut(s)	1411
BfuI	GTATCC	1 cut(s)	3536
BlpI	GCTNAGC	2 cut(s)	119, 2233
BmcAI	AGTACT	2 cut(s)	185, 931
Bme1390I	CCNGG	4 cut(s)	437, 540, 1848, 2398
Bme18I	GGWCC	3 cut(s)	252, 1254, 1850
BmeT110I	CYCGRG	2 cut(s)	2728, 3501
BmgT120I	GGNCC	4 cut(s)	252, 1254, 1850, 2726
BmiI	GGNNCC	4 cut(s)	1256, 1684, 3223, 3742
BmrFI	CCNGG	4 cut(s)	437, 540, 1848, 2398
BmrI	ACTGGG	3 cut(s)	1978, 2099, 3074
BmsI	GCATC	6 cut(s)	379, 1107, 1528, 1589, 2704, 2908
BmuI	ACTGGG	3 cut(s)	1978, 2099, 3074
BpmI	CTGGAG	5 cut(s)	627, 1470, 1743, 2249, 3614
Bpu10I	CCTNAGC	3 cut(s)	1365, 1619, 2146
Bpu1102I	GCTNAGC	2 cut(s)	119, 2233
Bpu14I	TTCGAA	1 cut(s)	2314
BpuEI	CTTGAG	6 cut(s)	176, 719, 1404, 1882, 3104, 3292
BpuMI	CCSGG	2 cut(s)	540, 2398
BsaAI	YACGTR	1 cut(s)	1631
BsaI	GGTCTC	1 cut(s)	1488
BsaJI	CCNNGG	4 cut(s)	538, 1846, 2729, 3420
BsaWI	WCCGGW	1 cut(s)	970
BsaXI	ACNNNNNCTCC	2 cut(s)	1855, 1885
Bsc4I	CCNNNNNNNGG	7 cut(s)	544, 545, 1196, 1263, 1859, 3218, 3535
Bse21I	CCTNAGG	1 cut(s)	311
Bse3DI	GCAATG	2 cut(s)	690, 2910
BseBI	CCWGG	2 cut(s)	437, 1848
BseDI	CCNNGG	4 cut(s)	538, 1846, 2729, 3420
BseGI	GGATG	8 cut(s)	275, 534, 1298, 1543, 1971, 2886, 3245, 3544
BseLI	CCNNNNNNNGG	7 cut(s)	544, 545, 1196, 1263, 1859, 3218, 3535
BseMI	GCAATG	2 cut(s)	690, 2910
BseRI	GAGGAG	3 cut(s)	2252, 3026, 3471
BseSI	GKGCMC	2 cut(s)	2398, 3556
BseYI	CCCAGC	2 cut(s)	1120, 1924
BsgI	GTGCAG	2 cut(s)	1226, 1380
Bsh1236I	CGCG	2 cut(s)	126, 966
BshFI	GGCC	5 cut(s)	840, 884, 2727, 3088, 3477
BshNI	GGYRCC	1 cut(s)	3740
BsiHKAI	GWGCWC	2 cut(s)	120, 2900
BsiHKCI	CYCGRG	2 cut(s)	2728, 3501
BsiSI	CCGG	3 cut(s)	540, 971, 2398
BslFI	GGGAC	2 cut(s)	1240, 3168
BslI	CCNNNNNNNGG	7 cut(s)	544, 545, 1196, 1263, 1859, 3218, 3535
BsmAI	GTCTC	2 cut(s)	262, 1488
BsmFI	GGGAC	2 cut(s)	1240, 3168
BsmI	GAATGC	2 cut(s)	898, 2068
BsnI	GGCC	5 cut(s)	840, 884, 2727, 3088, 3477
Bso31I	GGTCTC	1 cut(s)	1488
BsoBI	CYCGRG	2 cut(s)	2728, 3501
Bsp119I	TTCGAA	1 cut(s)	2314
Bsp1286I	GDGCHC	4 cut(s)	120, 2398, 2900, 3556
Bsp1407I	TGTACA	1 cut(s)	1558
Bsp1720I	GCTNAGC	2 cut(s)	119, 2233
Bsp19I	CCATGG	1 cut(s)	3420
BspACI	CCGC	5 cut(s)	124, 640, 1615, 2762, 3671
BspANI	GGCC	5 cut(s)	840, 884, 2727, 3088, 3477
BspFNI	CGCG	2 cut(s)	126, 966
BspHI	TCATGA	2 cut(s)	2529, 3403
BspLI	GGNNCC	4 cut(s)	1256, 1684, 3223, 3742
BspMAI	CTGCAG	2 cut(s)	1408, 2440
BspMI	ACCTGC	1 cut(s)	1411
BspPI	GGATC	9 cut(s)	1181, 1677, 1690, 2182, 2421, 3203, 3216, 3229, 3712
BspQI	GCTCTTC	1 cut(s)	516
BspT104I	TTCGAA	1 cut(s)	2314
BspT107I	GGYRCC	1 cut(s)	3740
BspTI	CTTAAG	1 cut(s)	1097
BspTNI	GGTCTC	1 cut(s)	1488
BsrDI	GCAATG	2 cut(s)	690, 2910
BsrGI	TGTACA	1 cut(s)	1558
BssECI	CCNNGG	4 cut(s)	538, 1846, 2729, 3420
BssT1I	CCWWGG	1 cut(s)	3420
Bst2UI	CCWGG	2 cut(s)	437, 1848
Bst4CI	ACNGT	7 cut(s)	338, 646, 2155, 2444, 2751, 3181, 3364
Bst6I	CTCTTC	5 cut(s)	388, 516, 637, 2127, 2582
BstAFI	CTTAAG	1 cut(s)	1097
BstAPI	GCANNNNNTGC	4 cut(s)	506, 932, 1529, 3551
BstAUI	TGTACA	1 cut(s)	1558
BstBAI	YACGTR	1 cut(s)	1631
BstBI	TTCGAA	1 cut(s)	2314
BstC8I	GCNNGC	4 cut(s)	1835, 1904, 2082, 2110
BstDSI	CCRYGG	1 cut(s)	3420
BstF5I	GGATG	8 cut(s)	275, 534, 1298, 1543, 1971, 2886, 3245, 3544
BstFNI	CGCG	2 cut(s)	126, 966
BstMAI	GTCTC	2 cut(s)	262, 1488
BstNI	CCWGG	2 cut(s)	437, 1848
BstNSI	RCATGY	1 cut(s)	3621
BstSCI	CCNGG	4 cut(s)	435, 538, 1846, 2396
BstSFI	CTRYAG	4 cut(s)	1404, 2436, 2622, 2805
BstSLI	GKGCMC	2 cut(s)	2398, 3556
BstSNI	TACGTA	1 cut(s)	1631
BstUI	CGCG	2 cut(s)	126, 966
BstX2I	RGATCY	3 cut(s)	1682, 2426, 3221
BstXI	CCANNNNNNTGG	1 cut(s)	1786
BstYI	RGATCY	3 cut(s)	1682, 2426, 3221
Bsu36I	CCTNAGG	1 cut(s)	311
BsuI	GTATCC	1 cut(s)	3536
BsuRI	GGCC	5 cut(s)	840, 884, 2727, 3088, 3477
BtgI	CCRYGG	1 cut(s)	3420
BtgZI	GCGATG	1 cut(s)	1566
BtsCI	GGATG	8 cut(s)	275, 534, 1298, 1543, 1971, 2886, 3245, 3544
BtsI	GCAGTG	2 cut(s)	1144, 2670
BveI	ACCTGC	1 cut(s)	1411
Cac8I	GCNNGC	4 cut(s)	1835, 1904, 2082, 2110
CaiI	CAGNNNCTG	1 cut(s)	3368
CciI	TCATGA	2 cut(s)	2529, 3403
Cfr13I	GGNCC	4 cut(s)	252, 1254, 1850, 2726
CseI	GACGC	2 cut(s)	115, 656
Csp6I	GTAC	3 cut(s)	184, 930, 1559
CspCI	CAANNNNNGTGG	2 cut(s)	3166, 3201
CviQI	GTAC	3 cut(s)	184, 930, 1559
DraI	TTTAAA	1 cut(s)	3106
DraIII	CACNNNGTG	1 cut(s)	1859
DrdI	GACNNNNNNGTC	1 cut(s)	1697
DseDI	GACNNNNNNGTC	1 cut(s)	1697
EaeI	YGGCCR	2 cut(s)	882, 3475
Eam1104I	CTCTTC	5 cut(s)	388, 516, 637, 2127, 2582
EarI	CTCTTC	5 cut(s)	388, 516, 637, 2127, 2582
Eco105I	TACGTA	1 cut(s)	1631
Eco130I	CCWWGG	1 cut(s)	3420
Eco147I	AGGCCT	1 cut(s)	3088
Eco31I	GGTCTC	1 cut(s)	1488
Eco32I	GATATC	1 cut(s)	1412
Eco47I	GGWCC	3 cut(s)	252, 1254, 1850
Eco57I	CTGAAG	6 cut(s)	698, 1005, 2108, 2430, 2455, 3390
Eco81I	CCTNAGG	1 cut(s)	311
Eco88I	CYCGRG	2 cut(s)	2728, 3501
EcoO109I	RGGNCCY	1 cut(s)	1254
EcoRII	CCWGG	2 cut(s)	435, 1846
EcoRV	GATATC	1 cut(s)	1412
EcoT14I	CCWWGG	1 cut(s)	3420
ErhI	CCWWGG	1 cut(s)	3420
FalI	AAGNNNNNCTT	4 cut(s)	1031, 1063, 2917, 2949
FaqI	GGGAC	2 cut(s)	1240, 3168
FauI	CCCGC	1 cut(s)	2755
FauNDI	CATATG	3 cut(s)	595, 1012, 1930
FbaI	TGATCA	1 cut(s)	2362
FblI	GTMKAC	1 cut(s)	177
FokI	GGATG	8 cut(s)	262, 521, 1285, 1550, 1958, 2873, 3252, 3551
FspBI	CTAG	5 cut(s)	2099, 2327, 2984, 3149, 3578
GsaI	CCCAGC	2 cut(s)	1124, 1928
GsuI	CTGGAG	5 cut(s)	627, 1470, 1743, 2249, 3614
HaeIII	GGCC	5 cut(s)	840, 884, 2727, 3088, 3477
HapII	CCGG	3 cut(s)	540, 971, 2398
HgaI	GACGC	2 cut(s)	115, 656
HincII	GTYRAC	6 cut(s)	43, 178, 1045, 1155, 1702, 2633
HindII	GTYRAC	6 cut(s)	43, 178, 1045, 1155, 1702, 2633
HindIII	AAGCTT	1 cut(s)	2560
HpaII	CCGG	3 cut(s)	540, 971, 2398
HphI	GGTGA	6 cut(s)	434, 839, 1604, 1930, 2956, 3685
Hpy166II	GTNNAC	8 cut(s)	43, 178, 1045, 1155, 1702, 1894, 2633, 3645
Hpy8I	GTNNAC	8 cut(s)	43, 178, 1045, 1155, 1702, 1894, 2633, 3645
Hpy99I	CGWCG	1 cut(s)	182
HpyAV	CCTTC	9 cut(s)	233, 370, 550, 922, 1321, 1325, 1672, 3373, 3539
HpyCH4III	ACNGT	7 cut(s)	338, 646, 2155, 2444, 2751, 3181, 3364
HpyCH4IV	ACGT	2 cut(s)	321, 1630
HpySE526I	ACGT	2 cut(s)	321, 1630
Ksp22I	TGATCA	1 cut(s)	2362
LguI	GCTCTTC	1 cut(s)	516
LmnI	GCTCC	8 cut(s)	143, 664, 756, 1334, 1724, 1789, 3013, 3422
LweI	GCATC	6 cut(s)	379, 1107, 1528, 1589, 2704, 2908
MaeI	CTAG	5 cut(s)	2099, 2327, 2984, 3149, 3578
MaeII	ACGT	2 cut(s)	321, 1630
MaeIII	GTNAC	9 cut(s)	48, 706, 1351, 1772, 1936, 2506, 3044, 3242, 3276
MfeI	CAATTG	3 cut(s)	210, 1442, 3726
MflI	RGATCY	3 cut(s)	1682, 2426, 3221
MhlI	GDGCHC	4 cut(s)	120, 2398, 2900, 3556
MlsI	TGGCCA	2 cut(s)	884, 3477
MluNI	TGGCCA	2 cut(s)	884, 3477
MlyI	GAGTC	3 cut(s)	974, 1083, 3656
MmeI	TCCRAC	4 cut(s)	199, 230, 781, 1111
Mox20I	TGGCCA	2 cut(s)	884, 3477
MroXI	GAANNNNTTC	4 cut(s)	683, 1284, 2310, 2334
MscI	TGGCCA	2 cut(s)	884, 3477
MslI	CAYNNNNRTG	7 cut(s)	764, 2041, 2071, 2092, 2528, 2891, 3671
Msp20I	TGGCCA	2 cut(s)	884, 3477
MspA1I	CMGCKG	2 cut(s)	1240, 2801
MspCI	CTTAAG	1 cut(s)	1097
MspI	CCGG	3 cut(s)	540, 971, 2398
MspR9I	CCNGG	4 cut(s)	437, 540, 1848, 2398
MunI	CAATTG	3 cut(s)	210, 1442, 3726
Mva1269I	GAATGC	2 cut(s)	898, 2068
MvaI	CCWGG	2 cut(s)	437, 1848
MvnI	CGCG	2 cut(s)	126, 966
NciI	CCSGG	2 cut(s)	540, 2398
NcoI	CCATGG	1 cut(s)	3420
NdeI	CATATG	3 cut(s)	595, 1012, 1930
NlaIV	GGNNCC	4 cut(s)	1256, 1684, 3223, 3742
NmuCI	GTSAC	5 cut(s)	1351, 1936, 2506, 3242, 3276
NspI	RCATGY	1 cut(s)	3621
NspV	TTCGAA	1 cut(s)	2314
OliI	CACNNNNGTG	2 cut(s)	2071, 2092
PagI	TCATGA	2 cut(s)	2529, 3403
PceI	AGGCCT	1 cut(s)	3088
PciI	ACATGT	1 cut(s)	3617
PciSI	GCTCTTC	1 cut(s)	516
PcsI	WCGNNNNNNNCGW	1 cut(s)	174
PctI	GAATGC	2 cut(s)	898, 2068
PdmI	GAANNNNTTC	4 cut(s)	683, 1284, 2310, 2334
PfeI	GAWTC	8 cut(s)	732, 858, 1285, 2183, 2208, 2842, 3115, 3574
PflFI	GACNNNGTC	1 cut(s)	173
PflMI	CCANNNNNTGG	2 cut(s)	1859, 3218
PleI	GAGTC	3 cut(s)	974, 1083, 3656
PpsI	GAGTC	3 cut(s)	974, 1083, 3656
Ppu21I	YACGTR	1 cut(s)	1631
PpuMI	RGGWCCY	1 cut(s)	1254
PscI	ACATGT	1 cut(s)	3617
PshBI	ATTAAT	1 cut(s)	2501
PsiI	TTATAA	1 cut(s)	3413
Psp5II	RGGWCCY	1 cut(s)	1254
Psp6I	CCWGG	2 cut(s)	435, 1846
PspFI	CCCAGC	2 cut(s)	1120, 1924
PspGI	CCWGG	2 cut(s)	435, 1846
PspN4I	GGNNCC	4 cut(s)	1256, 1684, 3223, 3742
PspPI	GGNCC	4 cut(s)	252, 1254, 1850, 2726
PspPPI	RGGWCCY	1 cut(s)	1254
PstI	CTGCAG	2 cut(s)	1408, 2440
PstNI	CAGNNNCTG	1 cut(s)	3368
PsuI	RGATCY	3 cut(s)	1682, 2426, 3221
PsyI	GACNNNGTC	1 cut(s)	173
PvuII	CAGCTG	2 cut(s)	1240, 2801
RsaI	GTAC	3 cut(s)	185, 931, 1560
RsaNI	GTAC	3 cut(s)	184, 930, 1559
RseI	CAYNNNNRTG	7 cut(s)	764, 2041, 2071, 2092, 2528, 2891, 3671
SalI	GTCGAC	1 cut(s)	176
SapI	GCTCTTC	1 cut(s)	516
Sau96I	GGNCC	4 cut(s)	252, 1254, 1850, 2726
SbfI	CCTGCAGG	1 cut(s)	1408
ScaI	AGTACT	2 cut(s)	185, 931
SchI	GAGTC	3 cut(s)	974, 1083, 3656
ScrFI	CCNGG	4 cut(s)	437, 540, 1848, 2398
SdaI	CCTGCAGG	1 cut(s)	1408
SduI	GDGCHC	4 cut(s)	120, 2398, 2900, 3556
SfaNI	GCATC	6 cut(s)	379, 1107, 1528, 1589, 2704, 2908
SfcI	CTRYAG	4 cut(s)	1404, 2436, 2622, 2805
SfuI	TTCGAA	1 cut(s)	2314
SinI	GGWCC	3 cut(s)	252, 1254, 1850
SmiMI	CAYNNNNRTG	7 cut(s)	764, 2041, 2071, 2092, 2528, 2891, 3671
SmlI	CTYRAG	7 cut(s)	191, 698, 1097, 1419, 1897, 3119, 3271
SmoI	CTYRAG	7 cut(s)	191, 698, 1097, 1419, 1897, 3119, 3271
SnaBI	TACGTA	1 cut(s)	1631
SpeI	ACTAGT	1 cut(s)	3148
Sse8387I	CCTGCAGG	1 cut(s)	1408
SseBI	AGGCCT	1 cut(s)	3088
SsiI	CCGC	5 cut(s)	124, 640, 1615, 2762, 3671
SspI	AATATT	3 cut(s)	1315, 1947, 2973
SspMI	CTAG	5 cut(s)	2099, 2327, 2984, 3149, 3578
StuI	AGGCCT	1 cut(s)	3088
StyD4I	CCNGG	4 cut(s)	435, 538, 1846, 2396
StyI	CCWWGG	1 cut(s)	3420
TaaI	ACNGT	7 cut(s)	338, 646, 2155, 2444, 2751, 3181, 3364
TaiI	ACGT	2 cut(s)	324, 1633
TaqI	TCGA	4 cut(s)	177, 2314, 2597, 2710
TaqII	GACCGA	1 cut(s)	511
TatI	WGTACW	3 cut(s)	183, 929, 1558
TauI	GCSGC	1 cut(s)	126
TfiI	GAWTC	8 cut(s)	732, 858, 1285, 2183, 2208, 2842, 3115, 3574
TseFI	GTSAC	5 cut(s)	1351, 1936, 2506, 3242, 3276
Tsp45I	GTSAC	5 cut(s)	1351, 1936, 2506, 3242, 3276
TspGWI	ACGGA	1 cut(s)	3214
Tth111I	GACNNNGTC	1 cut(s)	173
Van91I	CCANNNNNTGG	2 cut(s)	1859, 3218
Vha464I	CTTAAG	1 cut(s)	1097
VpaK11BI	GGWCC	3 cut(s)	252, 1254, 1850
VspI	ATTAAT	1 cut(s)	2501
XapI	RAATTY	3 cut(s)	346, 1203, 1503
XbaI	TCTAGA	1 cut(s)	2983
XceI	RCATGY	1 cut(s)	3621
XcmI	CCANNNNNNNNNTGG	1 cut(s)	286
XmiI	GTMKAC	1 cut(s)	177
XmnI	GAANNNNTTC	4 cut(s)	683, 1284, 2310, 2334
XspI	CTAG	5 cut(s)	2099, 2327, 2984, 3149, 3578
ZrmI	AGTACT	2 cut(s)	185, 931

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.