Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Reverse (-)

64137184 .. 64142922

Gene Structure 5739 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ41042

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3378 bp

ATGGGGAAGACAATACAGTTATATGGATTCCCTTCTGGTGAATCTGCCGAAAAAATTAAGGTATTTTTGGAGAGACATACAGGAGAAGGAAGTGTTTTTGCTCTTAAATTACGGCCGGCCAAAAACGGGGGAAGATTCTATGCTATCATACAGTTTCAGTCCACCCATCAGGCTGAGATTATCACCTCTTTGGCTAAAGCTAGGCTGTGGTATGGGAGATCATATTTGAACGCTCGTCCGGTGGACCTTGATATTGTACCAAAGCCAAGAACTTTTATGCATAGCTTGCAAAATTTAGTATTGCATGTTGGCTGCCAGGTTGCGAATGATAAGTTGTCTGTTTTGTGGAGAACGGCTGAAGTTTCAGTGGACTTTGGGATTGGAATGAGGAAATTGCAATTTCGGCTGTTATATCGTCAGAAGTTATATAGACTTCAGCTCTCTTATGAGAACATCTGGCAAATTGAGTTGCATCGTCCACGTGGTTGCACTGCAAAATATCTTGTCATTCAGTTATATGGAGCTCCTCGCATCTACCAACAAGAAGAAGCATGTTCTTCTGGAAATGTATATGACGACCCTCTTATGAACTACTTTAGGGATGTCTCTGATGACCAATGGGTTCGGACCACTGATTTCACTCCACGAAGCTGTATTGGGGAGTCATCTTCATTATGTTTAGAGCTTCCTTCTGGTCGCCAACTTCCTGATTTTGAAGAAAATTTTGCTTATTATAAGGAGAGTGATGGGCAATTTATCTTGGAGAATGGCTCAACTTTCTCTTGCAACCGAGATCTGGTCCCTATTGTTGGTTCTCCTGCAGGGGTTGATTTGCCATATGATATCTTGTTTAAAATAAACTTGCTGGTTCAAATGGGTTGTCTATCAGGGCCGACACTGGATAATAGTTTTTATAGGCTGGTGGATCCACGAAGAATAACTTGTTCCTGTATAGAGTATGCCCTAGATAAATTATCTAATTTGAAAGAGAGCTGTTATGAACCTGCAGTGTGGCTTCATGAGGAATATCGGAAGTACCTGACATCAAAGAAACCTCCAAAGCCGGCACTTATCTCCTTAGATTCTGGTTTGGTATATGTACGCAGGGTTCAAATAACACCATCTAAAGTGTATTTCTCTGGTCCTGAGGTTAATGTCTCAAATCGCATACTGCGCCAGTATTCCAGTGATATCGATAACTTTCTTCGTGTTTCATTTGTTGATGAGGAGATGGAAAAACTCTATTCAACAGATTTATCCCCCCGTACATCTACTAGGAAAACTCGCATCTACAACAGAATTCTTTCTATTCTCAGAAACGGCATAGTAATTGGAGAAAAAAAATTTGAGTTTCTTGCATTCTCATCAAGTCAATTGCGAGAGAATTCGTTGTGGATGTTTGCTTCAACCTATAAAGTTAGTGCTGATAAGATAAGAGCAGGGATGGGGGATTTTCGCAAGATAAGAAACGTGGCAAAGTATGCTGCCAGATTGGGTCAATCTTTTGGCTCTTCTACTGAAACTCTAAGTGTTGCCAGGAGTGAAATTGAAGTTATTCCTGATGTAACCGTTATCTGGGGTGGAGTCAAATATGTGTTCTCCGATGGGATTGGGAAAATATCAGCTGATTTTGCAAAGAGAGTAGCCTCCAAATGTGGCTATAAAGCGTTCACACCATCTGCATTTCAAATTCGATATGGTGGTTACAAAGGTGTTGTAGCTGTTGATCCAACCTCGTCAATGAAATTATCACTTAGAAAGAGCATGTCTAAGTATGATTCTGAAAACACAAAGCTAGATGTTCTAGCATGTAGCAAGTTTCAGCCTTGTTTTTTGAATCGTCAGTTGATAACTCTTCTGTCTACACTTGGAGTTCAAGATCATGTTTTCTTGAAAAAGCAAAGAGCAGCTGTACAACAACTTAATGCCATATTGACTGACCCATTAAAAGCAGAGGAGGCTCTTGATTTGATGTCTGCGGGTGAGAGTACAAACATCCTCAAGGAACTGCTGATGTGTGGTTATAAACCTGATGTTGAACCATTCCTTTCAATGATGCTCCAAACATTTCGGGCATCAAAGTTGTTGGAATTGAGGACCAAAACAAGAATCTTTATCCCAGACGGAAGAGCAATGATGGGATGTCTAGACGAAACAAGAACCTTGGAATATGGGCAGGTGTTTGTGCAAATCTCTGGAAACAGACAAAGGCAGTTATTTGATGATTCATTCATGCATAGTGGAAGCTCATCAGGCAACTGTTTTGTTATTGAGGGAAAGGTGGTTGTTGCAAAGAACCCATGTTTACACCCTGGTGATGTGCGTGTTTTAAGGGCTGTGAATGTGCCGGAGTTACACCATATGGTGGACTGTGTGGTATTTCCACAAAAAGGACCAAGGCCTCATCCAAATGAATGTTCTGGAAGTGATCTTGATGGAGATATTTACTTTGTTTGCTGGGACCCTGAGTTGATTCCTCGCATTCAAATCCAACCTATGGATTATACCCCAGCACCAACTGTAGAGATGGATCATGATGTCACAATTGAGGAAGTAGAAGAGTACTTTGTGAATTACATACTCAATGATAGTTTGGGGATAATTGCCAATGCCCACACTGTTTTTGCTGACAGAGAGTATGGTAAAGCAACGAGCACTCCATGCATAAAGCTCGCGAAGCTATTTTCAATTGCTGTAGACTTTCCAAAAACTGGCGTGCCAGCTGTGATACCACCAGAACTGTATGTCAAGGAATATCCAGATTTCATGGAGAAGCCCGACAAAAGCACCTATCTTTCAAGCAATGTGATCGGACAGCTATTTCGTGAGGTGAAGGACATTGCACCCCATGACAGCTGTATTACATCCTTCACTCTTCAAGTGGCAACAAAATCCTATGACCCTGACATGGAGGTTGATGACTTTGAGGATTACCTTGAAGATGCTTTCTATTACAAAGGCAATTATGATTACAAATTGGGAAACCTGTTGGATTACTATGGGATCAAGACAGAGGCTGAAATATTGAGTGGGAGTATTATGAGCATGTCAAAATCTTTCACCAAGAGGCGGGATGCAGAGGCTATAAACATGGCTGTGAAGTCATTGAGGAAGGAAGCTAGGGCCTGGTTCAATGAGAAAGGAACTGGAATAGATTCTGGCGATGATGTCTATGCAAAAGCCTCAGCTTGGTATTATGTCACATATCATCCCAAGTATTTTGGTTTGTACAATGAGGGAATGAAACGTGACCATTTTATCAGCTTTCCATGGTGTGTTTATGACAAGCTGGTTCACATAAAAAAGGACAAAGTAAGGCAAGCGCCGAGCATGTCATCTCTAGAAAGTCAGTTGAGGTATGGCTTGCATTTGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

1125

Amino Acids

127.81

Weight (kDa)

7.99

Isoelectric Point (pI)

38.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_RdRP1_2	PF26250	4 - 81	1.1e-16	RdRP1/2, RRM domain
PH_RDR2	PF24823	101 - 252	6.2e-45	RDR2 PH-like domain
RdRP_helical	PF26252	273 - 351	2.5e-10	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	372 - 944	6.9e-196	RNA dependent RNA polymerase
RdRP_head	PF26253	965 - 1107	1.7e-47	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	735, 2025
AarI	CACCTGC	1 cut(s)	2167
Acc36I	ACCTGC	2 cut(s)	1012, 2167
AccB7I	CCANNNNNTGG	3 cut(s)	2365, 2497, 2711
AccI	GTMKAC	2 cut(s)	1862, 2697
AccII	CGCG	1 cut(s)	2675
AciI	CCGC	2 cut(s)	1979, 3070
AclWI	GGATC	5 cut(s)	920, 933, 1721, 2538, 3011
AcoI	YGGCCR	2 cut(s)	113, 117
AcsI	RAATTY	6 cut(s)	292, 721, 1299, 1343, 1384, 1689
AcuI	CTGAAG	2 cut(s)	378, 419
AcvI	CACGTG	1 cut(s)	482
AfaI	GTAC	8 cut(s)	258, 1037, 1101, 1267, 1914, 1990, 2564, 3230
AjnI	CCWGG	4 cut(s)	315, 1535, 2311, 3125
AjuI	GAANNNNNNNTTGG	2 cut(s)	2401, 2433
AleI	CACNNNNGTG	1 cut(s)	2313
Alw21I	GWGCWC	2 cut(s)	526, 2657
Alw26I	GTCTC	3 cut(s)	67, 610, 1162
AlwI	GGATC	5 cut(s)	920, 933, 1721, 2538, 3011
AlwNI	CAGNNNCTG	1 cut(s)	3017
AoxI	GGCC	5 cut(s)	113, 117, 890, 2399, 3123
ApeKI	GCWGC	3 cut(s)	312, 1484, 1907
ApoI	RAATTY	6 cut(s)	292, 721, 1299, 1343, 1384, 1689
ArsI	GACNNNNNNTTYG	4 cut(s)	865, 897, 2805, 2837
Asp700I	GAANNNNTTC	3 cut(s)	1303, 1554, 3154
AspLEI	GCGC	2 cut(s)	1176, 3325
AspS9I	GGNCC	9 cut(s)	244, 627, 799, 890, 1142, 2097, 2393, 2461, 3123
AsuHPI	GGTGA	6 cut(s)	50, 175, 1994, 2327, 2842, 3052
AvaII	GGWCC	7 cut(s)	244, 627, 799, 1142, 2097, 2393, 2461
AxyI	CCTNAGG	1 cut(s)	1146
BamHI	GGATCC	1 cut(s)	925
BanII	GRGCYC	1 cut(s)	526
BbrPI	CACGTG	1 cut(s)	482
BbsI	GAAGAC	1 cut(s)	14
Bbv12I	GWGCWC	2 cut(s)	526, 2657
BbvCI	CCTCAGC	1 cut(s)	3184
BbvI	GCAGC	3 cut(s)	299, 1471, 1919
BceAI	ACGGC	3 cut(s)	128, 369, 1336
BcgI	CGANNNNNNTGC	4 cut(s)	2653, 2687, 2791, 2825
BciT130I	CCWGG	4 cut(s)	317, 1537, 2313, 3127
BcoDI	GTCTC	3 cut(s)	67, 610, 1162
BfaI	CTAG	8 cut(s)	201, 965, 1275, 1796, 1805, 2147, 3120, 3341
BfmI	CTRYAG	4 cut(s)	819, 1005, 2520, 2694
BfoI	RGCGCY	1 cut(s)	3326
BfuAI	ACCTGC	2 cut(s)	1012, 2167
BglII	AGATCT	1 cut(s)	793
BisI	GCNGC	3 cut(s)	313, 1485, 1908
BlsI	GCNGC	3 cut(s)	314, 1486, 1909
BmcAI	AGTACT	1 cut(s)	2564
Bme1390I	CCNGG	4 cut(s)	317, 1537, 2313, 3127
Bme18I	GGWCC	7 cut(s)	244, 627, 799, 1142, 2097, 2393, 2461
BmgT120I	GGNCC	9 cut(s)	244, 627, 799, 890, 1142, 2097, 2393, 2461, 3123
BmiI	GGNNCC	4 cut(s)	801, 927, 2462, 2463
BmrFI	CCNGG	4 cut(s)	317, 1537, 2313, 3127
BmsI	GCATC	7 cut(s)	481, 540, 1296, 2046, 2084, 2932, 3064
BpiI	GAAGAC	1 cut(s)	14
BplI	GAGNNNNNCTC	2 cut(s)	755, 787
Bpu10I	CCTNAGC	1 cut(s)	3184
BpuEI	CTTGAG	1 cut(s)	1985
Bsa29I	ATCGAT	1 cut(s)	1194
BsaAI	YACGTR	1 cut(s)	482
BsaJI	CCNNGG	4 cut(s)	2163, 2311, 2396, 3269
BsaWI	WCCGGW	1 cut(s)	238
BsaXI	ACNNNNNCTCC	2 cut(s)	2641, 2671
Bse118I	RCCGGY	2 cut(s)	115, 1063
Bse1I	ACTGG	5 cut(s)	903, 1177, 1185, 2716, 3151
Bse21I	CCTNAGG	1 cut(s)	1146
Bse3DI	GCAATG	3 cut(s)	2139, 2809, 2838
BseBI	CCWGG	4 cut(s)	317, 1537, 2313, 3127
BseCI	ATCGAT	1 cut(s)	1194
BseDI	CCNNGG	4 cut(s)	2163, 2311, 2396, 3269
BseGI	GGATG	9 cut(s)	607, 1401, 1449, 1995, 2147, 2404, 2864, 3079, 3208
BseMI	GCAATG	3 cut(s)	2139, 2809, 2838
BseMII	CTCAG	5 cut(s)	165, 1137, 1327, 2457, 3198
BseNI	ACTGG	5 cut(s)	903, 1177, 1185, 2716, 3151
BseRI	GAGGAG	3 cut(s)	516, 1241, 1970
BseX3I	CGGCCG	1 cut(s)	113
BseXI	GCAGC	3 cut(s)	299, 1471, 1919
BseYI	CCCAGC	2 cut(s)	2457, 2509
Bsh1236I	CGCG	1 cut(s)	2675
Bsh1285I	CGRYCG	1 cut(s)	116
BshFI	GGCC	5 cut(s)	115, 119, 892, 2401, 3125
BshVI	ATCGAT	1 cut(s)	1194
BsiEI	CGRYCG	1 cut(s)	116
BsiHKAI	GWGCWC	2 cut(s)	526, 2657
BsiSI	CCGG	4 cut(s)	116, 239, 1064, 2348
BslFI	GGGAC	2 cut(s)	785, 2474
BsmAI	GTCTC	3 cut(s)	67, 610, 1162
BsmFI	GGGAC	2 cut(s)	785, 2474
BsmI	GAATGC	2 cut(s)	1358, 2481
BsnI	GGCC	5 cut(s)	115, 119, 892, 2401, 3125
Bsp1286I	GDGCHC	2 cut(s)	526, 2657
Bsp1407I	TGTACA	2 cut(s)	1912, 3228
Bsp143I	GATC	9 cut(s)	218, 793, 925, 1726, 1879, 2428, 2530, 2808, 3003
Bsp19I	CCATGG	1 cut(s)	3269
Bsp68I	TCGCGA	1 cut(s)	2675
BspACI	CCGC	2 cut(s)	1979, 3070
BspANI	GGCC	5 cut(s)	115, 119, 892, 2401, 3125
BspCNI	CTCAG	5 cut(s)	166, 1138, 1326, 2458, 3197
BspDI	ATCGAT	1 cut(s)	1194
BspFNI	CGCG	1 cut(s)	2675
BspHI	TCATGA	2 cut(s)	1018, 2533
BspLI	GGNNCC	4 cut(s)	801, 927, 2462, 2463
BspMAI	CTGCAG	2 cut(s)	823, 1009
BspMI	ACCTGC	2 cut(s)	1012, 2167
BspPI	GGATC	5 cut(s)	920, 933, 1721, 2538, 3011
BspQI	GCTCTTC	2 cut(s)	1516, 2122
BsrDI	GCAATG	3 cut(s)	2139, 2809, 2838
BsrFI	RCCGGY	2 cut(s)	115, 1063
BsrGI	TGTACA	2 cut(s)	1912, 3228
BsrI	ACTGG	5 cut(s)	903, 1177, 1185, 2716, 3151
BssAI	RCCGGY	2 cut(s)	115, 1063
BssECI	CCNNGG	4 cut(s)	2163, 2311, 2396, 3269
BssMI	GATC	9 cut(s)	218, 793, 925, 1726, 1879, 2428, 2530, 2808, 3003
BssT1I	CCWWGG	3 cut(s)	2163, 2396, 3269
Bst2UI	CCWGG	4 cut(s)	317, 1537, 2313, 3127
Bst4CI	ACNGT	8 cut(s)	18, 153, 1570, 2261, 2372, 2521, 2620, 2742
Bst6I	CTCTTC	5 cut(s)	1516, 1860, 2122, 2553, 2880
BstAPI	GCANNNNNTGC	3 cut(s)	286, 1481, 2661
BstAUI	TGTACA	2 cut(s)	1912, 3228
BstBAI	YACGTR	1 cut(s)	482
BstC8I	GCNNGC	8 cut(s)	117, 287, 1065, 2673, 2717, 2721, 3321, 3365
BstDEI	CTNAG	9 cut(s)	174, 1078, 1146, 1313, 1526, 1754, 1770, 2466, 3184
BstDSI	CCRYGG	1 cut(s)	3269
BstF5I	GGATG	9 cut(s)	607, 1401, 1449, 1995, 2147, 2404, 2864, 3079, 3208
BstFNI	CGCG	1 cut(s)	2675
BstH2I	RGCGCY	1 cut(s)	3326
BstHHI	GCGC	2 cut(s)	1176, 3325
BstKTI	GATC	9 cut(s)	221, 796, 928, 1729, 1882, 2431, 2533, 2811, 3006
BstMAI	GTCTC	3 cut(s)	67, 610, 1162
BstMBI	GATC	9 cut(s)	218, 793, 925, 1726, 1879, 2428, 2530, 2808, 3003
BstMCI	CGRYCG	1 cut(s)	116
BstMWI	GCNNNNNNNGC	9 cut(s)	286, 403, 1173, 1481, 1631, 1958, 2253, 2661, 2677
BstNI	CCWGG	4 cut(s)	317, 1537, 2313, 3127
BstNSI	RCATGY	6 cut(s)	308, 555, 1768, 1812, 3049, 3334
BstSCI	CCNGG	4 cut(s)	315, 1535, 2311, 3125
BstSFI	CTRYAG	4 cut(s)	819, 1005, 2520, 2694
BstUI	CGCG	1 cut(s)	2675
BstV1I	GCAGC	3 cut(s)	299, 1471, 1919
BstV2I	GAAGAC	1 cut(s)	14
BstX2I	RGATCY	2 cut(s)	793, 925
BstYI	RGATCY	2 cut(s)	793, 925
BstZI	CGGCCG	1 cut(s)	113
Bsu15I	ATCGAT	1 cut(s)	1194
Bsu36I	CCTNAGG	1 cut(s)	1146
BsuRI	GGCC	5 cut(s)	115, 119, 892, 2401, 3125
BsuTUI	ATCGAT	1 cut(s)	1194
BtgI	CCRYGG	1 cut(s)	3269
BtgZI	GCGATG	1 cut(s)	3177
BtsCI	GGATG	9 cut(s)	607, 1401, 1449, 1995, 2147, 2404, 2864, 3079, 3208
BtsI	GCAGTG	2 cut(s)	489, 1014
BtsIMutI	CAGTG	7 cut(s)	372, 489, 630, 896, 1014, 1192, 2616
BtuMI	TCGCGA	1 cut(s)	2675
BveI	ACCTGC	2 cut(s)	1012, 2167
Cac8I	GCNNGC	8 cut(s)	117, 287, 1065, 2673, 2717, 2721, 3321, 3365
CaiI	CAGNNNCTG	1 cut(s)	3017
CciI	TCATGA	2 cut(s)	1018, 2533
CfoI	GCGC	2 cut(s)	1176, 3325
Cfr10I	RCCGGY	2 cut(s)	115, 1063
Cfr13I	GGNCC	9 cut(s)	244, 627, 799, 890, 1142, 2097, 2393, 2461, 3123
ClaI	ATCGAT	1 cut(s)	1194
Csp6I	GTAC	8 cut(s)	257, 1036, 1100, 1266, 1913, 1989, 2563, 3229
CviQI	GTAC	8 cut(s)	257, 1036, 1100, 1266, 1913, 1989, 2563, 3229
DdeI	CTNAG	9 cut(s)	174, 1078, 1146, 1313, 1526, 1754, 1770, 2466, 3184
DpnI	GATC	9 cut(s)	220, 795, 927, 1728, 1881, 2430, 2532, 2810, 3005
DpnII	GATC	9 cut(s)	218, 793, 925, 1726, 1879, 2428, 2530, 2808, 3003
DraI	TTTAAA	1 cut(s)	853
EaeI	YGGCCR	2 cut(s)	113, 117
EagI	CGGCCG	1 cut(s)	113
Eam1104I	CTCTTC	5 cut(s)	1516, 1860, 2122, 2553, 2880
EarI	CTCTTC	5 cut(s)	1516, 1860, 2122, 2553, 2880
Ecl136II	GAGCTC	1 cut(s)	524
EclXI	CGGCCG	1 cut(s)	113
Eco130I	CCWWGG	3 cut(s)	2163, 2396, 3269
Eco147I	AGGCCT	1 cut(s)	2401
Eco24I	GRGCYC	1 cut(s)	526
Eco32I	GATATC	2 cut(s)	844, 1192
Eco47I	GGWCC	7 cut(s)	244, 627, 799, 1142, 2097, 2393, 2461
Eco52I	CGGCCG	1 cut(s)	113
Eco53kI	GAGCTC	1 cut(s)	524
Eco57I	CTGAAG	2 cut(s)	378, 419
Eco72I	CACGTG	1 cut(s)	482
Eco81I	CCTNAGG	1 cut(s)	1146
EcoICRI	GAGCTC	1 cut(s)	524
EcoO109I	RGGNCCY	2 cut(s)	2461, 3123
EcoRI	GAATTC	2 cut(s)	1299, 1384
EcoRII	CCWGG	4 cut(s)	315, 1535, 2311, 3125
EcoRV	GATATC	2 cut(s)	844, 1192
EcoT14I	CCWWGG	3 cut(s)	2163, 2396, 3269
EcoT22I	ATGCAT	3 cut(s)	282, 2238, 2666
EcoT38I	GRGCYC	1 cut(s)	526
ErhI	CCWWGG	3 cut(s)	2163, 2396, 3269
FalI	AAGNNNNNCTT	2 cut(s)	925, 957
FaqI	GGGAC	2 cut(s)	785, 2474
FauI	CCCGC	2 cut(s)	1972, 3063
FauNDI	CATATG	2 cut(s)	838, 2361
FblI	GTMKAC	2 cut(s)	1862, 2697
Fnu4HI	GCNGC	3 cut(s)	313, 1485, 1908
FokI	GGATG	9 cut(s)	614, 1408, 1456, 1982, 2154, 2391, 2851, 3086, 3195
FriOI	GRGCYC	1 cut(s)	526
FseI	GGCCGGCC	1 cut(s)	119
Fsp4HI	GCNGC	3 cut(s)	313, 1485, 1908
FspBI	CTAG	8 cut(s)	201, 965, 1275, 1796, 1805, 2147, 3120, 3341
GlaI	GCGC	2 cut(s)	1175, 3324
GluI	GCNGC	3 cut(s)	313, 1485, 1908
GsaI	CCCAGC	2 cut(s)	2461, 2513
HaeII	RGCGCY	1 cut(s)	3326
HaeIII	GGCC	5 cut(s)	115, 119, 892, 2401, 3125
HapII	CCGG	4 cut(s)	116, 239, 1064, 2348
HhaI	GCGC	2 cut(s)	1176, 3325
Hin6I	GCGC	2 cut(s)	1174, 3323
HinP1I	GCGC	2 cut(s)	1174, 3323
HpaII	CCGG	4 cut(s)	116, 239, 1064, 2348
HphI	GGTGA	6 cut(s)	50, 175, 1994, 2327, 2842, 3052
Hpy188I	TCNGA	9 cut(s)	420, 610, 627, 1032, 1316, 1603, 1783, 2813, 3377
HpyAV	CCTTC	6 cut(s)	42, 80, 699, 2827, 2878, 3106
HpyCH4III	ACNGT	8 cut(s)	18, 153, 1570, 2261, 2372, 2521, 2620, 2742
HpyCH4IV	ACGT	3 cut(s)	481, 1470, 3247
HpyF10VI	GCNNNNNNNGC	9 cut(s)	286, 403, 1173, 1481, 1631, 1958, 2253, 2661, 2677
HpyF3I	CTNAG	9 cut(s)	174, 1078, 1146, 1313, 1526, 1754, 1770, 2466, 3184
HpySE526I	ACGT	3 cut(s)	481, 1470, 3247
HspAI	GCGC	2 cut(s)	1174, 3323
KflI	GGGWCCC	1 cut(s)	2461
KroI	GCCGGC	2 cut(s)	115, 1063
KroNI	GCCGGC	2 cut(s)	117, 1065
Kzo9I	GATC	9 cut(s)	218, 793, 925, 1726, 1879, 2428, 2530, 2808, 3003
LguI	GCTCTTC	2 cut(s)	1516, 2122
LmnI	GCTCC	3 cut(s)	521, 529, 2064
Lsp1109I	GCAGC	3 cut(s)	299, 1471, 1919
LweI	GCATC	7 cut(s)	481, 540, 1296, 2046, 2084, 2932, 3064
MaeI	CTAG	8 cut(s)	201, 965, 1275, 1796, 1805, 2147, 3120, 3341
MaeII	ACGT	3 cut(s)	481, 1470, 3247
MaeIII	GTNAC	6 cut(s)	1564, 1703, 2352, 2539, 3199, 3248
MalI	GATC	9 cut(s)	220, 795, 927, 1728, 1881, 2430, 2532, 2810, 3005
MboI	GATC	9 cut(s)	218, 793, 925, 1726, 1879, 2428, 2530, 2808, 3003
MfeI	CAATTG	3 cut(s)	1373, 2544, 2688
MflI	RGATCY	2 cut(s)	793, 925
MhlI	GDGCHC	2 cut(s)	526, 2657
MlyI	GAGTC	2 cut(s)	671, 1593
MmeI	TCCRAC	4 cut(s)	1754, 2067, 2515, 2970
Mph1103I	ATGCAT	3 cut(s)	282, 2238, 2666
MroNI	GCCGGC	2 cut(s)	115, 1063
MroXI	GAANNNNTTC	3 cut(s)	1303, 1554, 3154
MseI	TTAA	7 cut(s)	57, 105, 852, 1152, 1923, 1946, 2330
MslI	CAYNNNNRTG	3 cut(s)	2313, 2409, 3095
MspA1I	CMGCKG	4 cut(s)	1625, 1910, 2723, 2856
MspI	CCGG	4 cut(s)	116, 239, 1064, 2348
MspR9I	CCNGG	4 cut(s)	317, 1537, 2313, 3127
MunI	CAATTG	3 cut(s)	1373, 2544, 2688
Mva1269I	GAATGC	2 cut(s)	1358, 2481
MvaI	CCWGG	4 cut(s)	317, 1537, 2313, 3127
MvnI	CGCG	1 cut(s)	2675
MwoI	GCNNNNNNNGC	9 cut(s)	286, 403, 1173, 1481, 1631, 1958, 2253, 2661, 2677
NaeI	GCCGGC	2 cut(s)	117, 1065
NcoI	CCATGG	1 cut(s)	3269
NdeI	CATATG	2 cut(s)	838, 2361
NdeII	GATC	9 cut(s)	218, 793, 925, 1726, 1879, 2428, 2530, 2808, 3003
NgoMIV	GCCGGC	2 cut(s)	115, 1063
NlaIV	GGNNCC	4 cut(s)	801, 927, 2462, 2463
NmeAIII	GCCGAG	1 cut(s)	3351
NmuCI	GTSAC	3 cut(s)	2539, 3199, 3248
NruI	TCGCGA	1 cut(s)	2675
NsiI	ATGCAT	3 cut(s)	282, 2238, 2666
NspI	RCATGY	6 cut(s)	308, 555, 1768, 1812, 3049, 3334
OliI	CACNNNNGTG	1 cut(s)	2313
PagI	TCATGA	2 cut(s)	1018, 2533
PaqCI	CACCTGC	1 cut(s)	2167
PceI	AGGCCT	1 cut(s)	2401
PciSI	GCTCTTC	2 cut(s)	1516, 2122
PctI	GAATGC	2 cut(s)	1358, 2481
PdiI	GCCGGC	2 cut(s)	117, 1065
PdmI	GAANNNNTTC	3 cut(s)	1303, 1554, 3154
PflMI	CCANNNNNTGG	3 cut(s)	2365, 2497, 2711
PkrI	GCNGC	3 cut(s)	314, 1486, 1909
PleI	GAGTC	2 cut(s)	670, 1592
PmaCI	CACGTG	1 cut(s)	482
PmlI	CACGTG	1 cut(s)	482
PpsI	GAGTC	2 cut(s)	670, 1592
Ppu21I	YACGTR	1 cut(s)	482
PpuMI	RGGWCCY	1 cut(s)	2461
PsiI	TTATAA	2 cut(s)	735, 2025
Psp124BI	GAGCTC	1 cut(s)	526
Psp5II	RGGWCCY	1 cut(s)	2461
Psp6I	CCWGG	4 cut(s)	315, 1535, 2311, 3125
PspCI	CACGTG	1 cut(s)	482
PspFI	CCCAGC	2 cut(s)	2457, 2509
PspGI	CCWGG	4 cut(s)	315, 1535, 2311, 3125
PspN4I	GGNNCC	4 cut(s)	801, 927, 2462, 2463
PspPI	GGNCC	9 cut(s)	244, 627, 799, 890, 1142, 2097, 2393, 2461, 3123
PspPPI	RGGWCCY	1 cut(s)	2461
PsrI	GAACNNNNNNTAC	2 cut(s)	1092, 1124
PstI	CTGCAG	2 cut(s)	823, 1009
PstNI	CAGNNNCTG	1 cut(s)	3017
PsuI	RGATCY	2 cut(s)	793, 925
PvuII	CAGCTG	4 cut(s)	1625, 1910, 2723, 2856
RigI	GGCCGGCC	1 cut(s)	119
RruI	TCGCGA	1 cut(s)	2675
RsaI	GTAC	8 cut(s)	258, 1037, 1101, 1267, 1914, 1990, 2564, 3230
RsaNI	GTAC	8 cut(s)	257, 1036, 1100, 1266, 1913, 1989, 2563, 3229
RseI	CAYNNNNRTG	3 cut(s)	2313, 2409, 3095
SacI	GAGCTC	1 cut(s)	526
SapI	GCTCTTC	2 cut(s)	1516, 2122
SaqAI	TTAA	7 cut(s)	57, 105, 852, 1152, 1923, 1946, 2330
SatI	GCNGC	3 cut(s)	313, 1485, 1908
Sau3AI	GATC	9 cut(s)	218, 793, 925, 1726, 1879, 2428, 2530, 2808, 3003
Sau96I	GGNCC	9 cut(s)	244, 627, 799, 890, 1142, 2097, 2393, 2461, 3123
SbfI	CCTGCAGG	1 cut(s)	823
ScaI	AGTACT	1 cut(s)	2564
SchI	GAGTC	2 cut(s)	671, 1593
ScrFI	CCNGG	4 cut(s)	317, 1537, 2313, 3127
SdaI	CCTGCAGG	1 cut(s)	823
SduI	GDGCHC	2 cut(s)	526, 2657
SfaNI	GCATC	7 cut(s)	481, 540, 1296, 2046, 2084, 2932, 3064
SfcI	CTRYAG	4 cut(s)	819, 1005, 2520, 2694
SinI	GGWCC	7 cut(s)	244, 627, 799, 1142, 2097, 2393, 2461
SmiMI	CAYNNNNRTG	3 cut(s)	2313, 2409, 3095
SmlI	CTYRAG	1 cut(s)	2000
SmoI	CTYRAG	1 cut(s)	2000
Sse8387I	CCTGCAGG	1 cut(s)	823
SseBI	AGGCCT	1 cut(s)	2401
SsiI	CCGC	2 cut(s)	1979, 3070
SspI	AATATT	1 cut(s)	3024
SspMI	CTAG	8 cut(s)	201, 965, 1275, 1796, 1805, 2147, 3120, 3341
SstI	GAGCTC	1 cut(s)	526
StuI	AGGCCT	1 cut(s)	2401
StyD4I	CCNGG	4 cut(s)	315, 1535, 2311, 3125
StyI	CCWWGG	3 cut(s)	2163, 2396, 3269
TaaI	ACNGT	8 cut(s)	18, 153, 1570, 2261, 2372, 2521, 2620, 2742
TaiI	ACGT	3 cut(s)	484, 1473, 3250
TaqI	TCGA	2 cut(s)	1194, 1693
TatI	WGTACW	4 cut(s)	1912, 1988, 2562, 3228
Tru1I	TTAA	7 cut(s)	57, 105, 852, 1152, 1923, 1946, 2330
Tru9I	TTAA	7 cut(s)	57, 105, 852, 1152, 1923, 1946, 2330
TscAI	CASTG	7 cut(s)	372, 496, 637, 903, 1014, 1192, 2623
TseFI	GTSAC	3 cut(s)	2539, 3199, 3248
TseI	GCWGC	3 cut(s)	312, 1484, 1907
Tsp45I	GTSAC	3 cut(s)	2539, 3199, 3248
TspGWI	ACGGA	1 cut(s)	2139
TspRI	CASTG	7 cut(s)	372, 496, 637, 903, 1014, 1192, 2623
Van91I	CCANNNNNTGG	3 cut(s)	2365, 2497, 2711
VpaK11BI	GGWCC	7 cut(s)	244, 627, 799, 1142, 2097, 2393, 2461
XapI	RAATTY	6 cut(s)	292, 721, 1299, 1343, 1384, 1689
XbaI	TCTAGA	2 cut(s)	2146, 3340
XceI	RCATGY	6 cut(s)	308, 555, 1768, 1812, 3049, 3334
XmiI	GTMKAC	2 cut(s)	1862, 2697
XmnI	GAANNNNTTC	3 cut(s)	1303, 1554, 3154
XspI	CTAG	8 cut(s)	201, 965, 1275, 1796, 1805, 2147, 3120, 3341
ZrmI	AGTACT	1 cut(s)	2564
Zsp2I	ATGCAT	3 cut(s)	282, 2238, 2666

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.