Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Reverse (-)

1215588 .. 1227193

Gene Structure 11606 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1019300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3156 bp

ATGGCCAGTGCTAGAAACTTGTACTATGGCGCTTCCTATCTGAATTCTTGGAAGCTGGATCAGGATATTGTACCAAAGCCAAGAGGCTCTTTGCATACCTTTGAAAACATAAAGCTGGATTTTGGCTGCAAGATCGCAACAAATAAGCTATTTAGTTTCTGGAATATGAACAATGTTTCAGTGGATTTTGGGATTGGATTGCGGAAACTAGTTTGTCGTCTGAAATATGACGCTCTGGAGTATATGATCGAACTTTCCTATGAAAACATCTGGCAGATTGAGTTGCATCGTCCACGCGGTTGTGCTGCTAAGCATCTTCTGATTCAGATGTACGGAGCACCTCGGATTTCTAAGAAAGTCATACGCTCTTCAGGACAAGCATATGAAGATCTTGGTATGACCTATTTCATGGACTCTGATGATCAATGGGTCAGGACTACTGATTTCACTCCATCACGTGCTATTGGGCAGTCTTTTGCATTAAGCTTGGAGCTTCCTCATAGTCTCCAACTTCCAGATTTTCGAGAAAACTTTCCTGGGTATGAAGAGATTGAAGGTAACCTTGTCTTGGAGGTTGGTCCCCCTTATTCTTGCAACCCGAGTCTGGGTCCCATGGTTTGTCCTCCTCGAGGTGTCAATGTGTATGTGCCATATGATATCTTGTTTAAAGTTCATTCTCTGCTTCAGCTTGGGTATCTTTCAGGGCCAACACTTGATGTTATATTTTATAAGATGGTTGATCCGCACAGATTTGATCGTGCATGTATAGAGTATGCCCTAGAGAAATTATATAATTTGAAAGAGACCTGCTATAAACCTTCAGAGTGGCTTAATGAGCAGTACAGGAAGTACCTCACGTCAAAGAGACGTCCACAGTCATCTGTCATATCCTTAGATTCTGGGTTGGTATATGTACGCAGGGTCCAAATTACACCATGTCGTGTATTTTCTTCTGGTCCTGAGGTCAATGTCTCAAATCGTGTACTACGCAATTATCCAGATGATATTGATAACTTCATTCGTGTTTCCTTTGTTGACGAGGAGATGGATCGAGTTTATTCTTCAGATTTGCTTCCACGTACTTCTGCTGCCTATGAGGATGGGAAAACCGACATCTACAAGCGGATTCTTTTTATTCTCAGAAACGGCATAGTAATTGGTGAAAAGAAATTCGAGTTTCTCGCATTCTCATCAAGTCAATTGCGAGAGAATTCTATGTGGATGTTTGCTTCAAGACGGGGACTTACTGCAGCCAATATAAGAGAGTGGATGGGGGATTTTCGTCGTATAAAAAATGTAGCAAAATATGCTGCCAGATTAGGTCAATCCTTTGGTTCGTCCACTGAAACTTTAAATGTTGGAAAACATGAAATGGAAGTTATTCCCGATGTAGAAAACAATAAATATGTCTTCTCCGATGGGATTGGGAAAATATCTGCTGATTTTGCTAAGAAAGTGGCCTCAAAATGTGGCTTTAATGGGACTCCATCTGCCTTTCAGATTCGATATGGTGGGTACAAAGGTGTGGTGGCCGTTGATCCAACTTCATCAATGAAATTATCATTGAGGAGGAGCATGTCCAAGTATGAATCAGAAAACACAAAGTTAGACGTTTTAGCATGTAGCAAGATTCAACCTTGTTTTCTGAACCGCCAGTTGATAAGTCTTTTATCCACCCTTGGGGTTCCAGATCCTGCTTTCATGAAAAAACAAAAGGCAGCTGTACAACAATTGAATGCTATATTGACTGACCCACTAAAAGCACAAGAGGCTCTGGATTTGATGTCTGCAGGGGAGACTACCAACTGCTTGAAGGAATTGCTTATATGTGGTTATAAACCTGATGTTGAACCCTTCATTTCAATGATGCTGCAAACATTTCGGGCATCAAAGTTGCTAGAGTTGCGGACGAAAACAAGAATCTTTATTCCAGATGGACGAGCAATGATGGGATGTCTAGATGAAACCAGAACATTGGAATATGGTGAGGTGTTTGTGCAATTCTCTGGTAACAGGCATACTGTAAGTGCATCAAACCAGCGATTCATTATTGTGGGGAAGGTAGCTGTTGCTAAGAACCCTTGTTTGCACCCTGGCGATGTACGTGTTTTAAAGGCTGTGGATGTGCCAGAGTTGTACCATATGGTTGATTGTGTTGTTTTTCCCCAAAAAGGACCAAGACCTCATCCAAATGAATGTTCGGGAAGTGATCTTGATGGAGATATCTACTATGTCTGTTGGGACGAAGAACTAATTCCTCGGAAGCAAATCGAACCCATGGATTACACCCCAGCACCAACTATAGAATTGGATCATGATGTCACAATTGAGGAAGTTGAAGAATATTTTGTGAACTACATGGTCAATGATAGCTTGGGCATAATTGCCAATGCCCACACTGTGTTTGCAGACAAAGATCCCTTAAAAGCAATGAGCGCAGAATGTATAGAGCTTGCAAAGCTATTTTCAATTGCCGTTGACTTTCCAAAAACTGGTGTGCCGGCTGTAATACCTCCACATTTATATGCCAAGGAATATCCAGATTTCATGGAAAAACTTGACAAACCCACCTATCAGTCATCCAATGTAATTGGAGCTCTTTTTCGTGAGGTGAAGGACATTGCGCCTCATGAAAGCTCTATCACATCCTTCACTCGTCAAGTGGCGAAGCAGTCATACGACTCTGACATGGTAGTTGATGGCTATGAGGATTACATTGAAGATGCTATCTATTACAAGGGCAATTATGATTACAAGTTGGGAAATCTGATGGAGTATTATGGGATCAAGACAGAGGCTGAAATACTTAGTGGGAGTGTTATGAGAATGTCAAAATCTTTCACTAAGAGGCGGGATGCAGAGTCTATAAACGTGGCTGTGAGATCGTTGAGGAAGGAAGCTAGGGCGTGGTTCAATGAGAAGGGAACTGGTTTAGATTCCGGAGCTGATGATGTATATGCCAAAGCTTCAGCTTGGTACCATGTCACATATCATCCTGATTATTTTGGTACATACAACGAGGGGCTGAATCGTGACCATTTCATCAGCTTTCCATGGTGCGTTTATGACAAGCTCGTCCTCATCAAAAAGGATAAAGCAAGTATACGCATGTCATCTCTAGAAAGCCAATTCTGGGATGGGTTGCATTTGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

1052

Amino Acids

119.55

Weight (kDa)

6.32

Isoelectric Point (pI)

39.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_RDR2	PF24823	39 - 188	8.5e-38	RDR2 PH-like domain
RDR6_2nd	PF24577	81 - 163	5.8e-07	RNA-dependent RNA polymerase 6 second domain
RdRP_helical	PF26252	206 - 290	1.2e-08	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	310 - 871	1.6e-195	RNA dependent RNA polymerase
RdRP_head	PF26253	892 - 1033	8.3e-46	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	729, 1836
AasI	GACNNNNNNGTC	1 cut(s)	3074
AatII	GACGTC	1 cut(s)	871
AbsI	CCTCGAGG	1 cut(s)	627
Acc36I	ACCTGC	1 cut(s)	815
Acc65I	GGTACC	1 cut(s)	2976
AccB1I	GGYRCC	1 cut(s)	2976
AccB7I	CCANNNNNTGG	1 cut(s)	2492
AccI	GTMKAC	1 cut(s)	3103
AccII	CGCG	1 cut(s)	297
AccIII	TCCGGA	1 cut(s)	2939
AciI	CCGC	7 cut(s)	202, 297, 743, 1123, 1651, 1906, 2851
AclWI	GGATC	8 cut(s)	66, 734, 1056, 1532, 1685, 2319, 2411, 2792
AcoI	YGGCCR	2 cut(s)	3, 1530
AcsI	RAATTY	3 cut(s)	43, 1169, 1210
AcuI	CTGAAG	5 cut(s)	354, 668, 804, 1047, 2952
AcvI	CACGTG	1 cut(s)	458
AcyI	GRCGYC	1 cut(s)	868
AdeI	CACNNNGTG	1 cut(s)	2401
AfiI	CCNNNNNNNGG	8 cut(s)	568, 604, 605, 629, 1680, 2171, 2492, 3133
AflIII	ACRYGT	1 cut(s)	2104
AhlI	ACTAGT	1 cut(s)	208
AjiI	CACGTC	1 cut(s)	858
AjnI	CCWGG	2 cut(s)	535, 2092
AjuI	GAANNNNNNNTTGG	2 cut(s)	2182, 2214
Alw21I	GWGCWC	2 cut(s)	340, 2599
Alw26I	GTCTC	5 cut(s)	509, 797, 859, 976, 1790
AlwI	GGATC	8 cut(s)	66, 734, 1056, 1532, 1685, 2319, 2411, 2792
AlwNI	CAGNNNCTG	2 cut(s)	1694, 2798
Ama87I	CYCGRG	2 cut(s)	598, 627
Aor13HI	TCCGGA	1 cut(s)	2939
AoxI	GGCC	4 cut(s)	3, 704, 1458, 1530
ApeKI	GCWGC	7 cut(s)	126, 305, 1088, 1250, 1310, 1718, 1870
ApoI	RAATTY	3 cut(s)	43, 1169, 1210
Asp700I	GAANNNNTTC	3 cut(s)	531, 1380, 2253
Asp718I	GGTACC	1 cut(s)	2976
AspLEI	GCGC	3 cut(s)	32, 2438, 2626
AspS9I	GGNCC	6 cut(s)	578, 608, 704, 922, 956, 2174
AsuHPI	GGTGA	3 cut(s)	1172, 1997, 2623
AvaI	CYCGRG	2 cut(s)	598, 627
AvaII	GGWCC	5 cut(s)	578, 608, 922, 956, 2174
AxyI	CCTNAGG	1 cut(s)	960
BaeI	ACNNNNGTAYC	2 cut(s)	1507, 1540
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	2976
BanII	GRGCYC	1 cut(s)	2599
BarI	GAAGNNNNNNTAC	2 cut(s)	2660, 2692
BbrPI	CACGTG	1 cut(s)	458
BbsI	GAAGAC	1 cut(s)	1402
Bbv12I	GWGCWC	2 cut(s)	340, 2599
BbvI	GCAGC	7 cut(s)	113, 292, 1075, 1262, 1297, 1730, 1857
BceAI	ACGGC	3 cut(s)	1162, 1517, 2459
BcgI	CGANNNNNNTGC	2 cut(s)	1862, 1896
BciT130I	CCWGG	2 cut(s)	537, 2094
BclI	TGATCA	1 cut(s)	421
BcoDI	GTCTC	5 cut(s)	509, 797, 859, 976, 1790
BcuI	ACTAGT	1 cut(s)	208
BfaI	CTAG	7 cut(s)	12, 209, 779, 1898, 1958, 2901, 3119
BfmI	CTRYAG	3 cut(s)	1248, 1788, 2301
BfoI	RGCGCY	1 cut(s)	33
BfuAI	ACCTGC	1 cut(s)	815
BglII	AGATCT	1 cut(s)	388
BisI	GCNGC	7 cut(s)	127, 306, 1089, 1251, 1311, 1719, 1871
BlpI	GCTNAGC	1 cut(s)	309
BlsI	GCNGC	7 cut(s)	128, 307, 1090, 1252, 1312, 1720, 1872
Bme1390I	CCNGG	2 cut(s)	537, 2094
Bme18I	GGWCC	5 cut(s)	578, 608, 922, 956, 2174
BmeT110I	CYCGRG	2 cut(s)	598, 627
BmgBI	CACGTC	1 cut(s)	858
BmgT120I	GGNCC	6 cut(s)	578, 608, 704, 922, 956, 2174
BmiI	GGNNCC	6 cut(s)	580, 609, 610, 923, 1686, 2978
BmrFI	CCNGG	2 cut(s)	537, 2094
BmsI	GCATC	7 cut(s)	295, 322, 1857, 1895, 2039, 2713, 2845
BpiI	GAAGAC	1 cut(s)	1402
BpmI	CTGGAG	1 cut(s)	257
Bpu1102I	GCTNAGC	1 cut(s)	309
BsaAI	YACGTR	3 cut(s)	458, 1079, 2105
BsaHI	GRCGYC	1 cut(s)	868
BsaI	GGTCTC	1 cut(s)	797
BsaJI	CCNNGG	9 cut(s)	341, 536, 612, 1678, 2092, 2258, 2277, 2529, 3053
BsaWI	WCCGGW	1 cut(s)	2939
Bsc4I	CCNNNNNNNGG	8 cut(s)	568, 604, 605, 629, 1680, 2171, 2492, 3133
Bse118I	RCCGGY	1 cut(s)	2500
Bse1I	ACTGG	4 cut(s)	6, 1654, 2497, 2932
Bse21I	CCTNAGG	1 cut(s)	960
Bse3DI	GCAATG	3 cut(s)	1950, 2436, 2619
BseAI	TCCGGA	1 cut(s)	2939
BseBI	CCWGG	2 cut(s)	537, 2094
BseDI	CCNNGG	9 cut(s)	341, 536, 612, 1678, 2092, 2258, 2277, 2529, 3053
BseLI	CCNNNNNNNGG	8 cut(s)	568, 604, 605, 629, 1680, 2171, 2492, 3133
BseMI	GCAATG	3 cut(s)	1950, 2436, 2619
BseMII	CTCAG	2 cut(s)	951, 1153
BseNI	ACTGG	4 cut(s)	6, 1654, 2497, 2932
BseRI	GAGGAG	4 cut(s)	615, 1055, 1582, 1585
BseXI	GCAGC	7 cut(s)	113, 292, 1075, 1262, 1297, 1730, 1857
BseYI	CCCAGC	1 cut(s)	2290
Bsh1236I	CGCG	1 cut(s)	297
BshFI	GGCC	4 cut(s)	5, 706, 1460, 1532
BshNI	GGYRCC	1 cut(s)	2976
BsiHKAI	GWGCWC	2 cut(s)	340, 2599
BsiHKCI	CYCGRG	2 cut(s)	598, 627
BsiSI	CCGG	2 cut(s)	2501, 2940
BslFI	GGGAC	5 cut(s)	564, 594, 1254, 1495, 2255
BslI	CCNNNNNNNGG	8 cut(s)	568, 604, 605, 629, 1680, 2171, 2492, 3133
BsmAI	GTCTC	5 cut(s)	509, 797, 859, 976, 1790
BsmBI	CGTCTC	1 cut(s)	859
BsmFI	GGGAC	5 cut(s)	564, 594, 1254, 1495, 2255
BsmI	GAATGC	2 cut(s)	1184, 1741
BsnI	GGCC	4 cut(s)	5, 706, 1460, 1532
Bso31I	GGTCTC	1 cut(s)	797
BsoBI	CYCGRG	2 cut(s)	598, 627
Bsp1286I	GDGCHC	2 cut(s)	340, 2599
Bsp13I	TCCGGA	1 cut(s)	2939
Bsp1407I	TGTACA	1 cut(s)	1723
Bsp1720I	GCTNAGC	1 cut(s)	309
Bsp19I	CCATGG	3 cut(s)	612, 2277, 3053
BspACI	CCGC	7 cut(s)	202, 297, 743, 1123, 1651, 1906, 2851
BspANI	GGCC	4 cut(s)	5, 706, 1460, 1532
BspCNI	CTCAG	2 cut(s)	952, 1152
BspEI	TCCGGA	1 cut(s)	2939
BspFNI	CGCG	1 cut(s)	297
BspHI	TCATGA	3 cut(s)	1701, 2314, 2629
BspLI	GGNNCC	6 cut(s)	580, 609, 610, 923, 1686, 2978
BspMAI	CTGCAG	2 cut(s)	1252, 1792
BspMI	ACCTGC	1 cut(s)	815
BspPI	GGATC	8 cut(s)	66, 734, 1056, 1532, 1685, 2319, 2411, 2792
BspQI	GCTCTTC	1 cut(s)	373
BspT107I	GGYRCC	1 cut(s)	2976
BspTNI	GGTCTC	1 cut(s)	797
BsrDI	GCAATG	3 cut(s)	1950, 2436, 2619
BsrFI	RCCGGY	1 cut(s)	2500
BsrGI	TGTACA	1 cut(s)	1723
BsrI	ACTGG	4 cut(s)	6, 1654, 2497, 2932
BssAI	RCCGGY	1 cut(s)	2500
BssECI	CCNNGG	9 cut(s)	341, 536, 612, 1678, 2092, 2258, 2277, 2529, 3053
BssNAI	GTATAC	1 cut(s)	3104
BssNI	GRCGYC	1 cut(s)	868
BssT1I	CCWWGG	5 cut(s)	612, 1678, 2277, 2529, 3053
Bst1107I	GTATAC	1 cut(s)	3104
Bst2UI	CCWGG	2 cut(s)	537, 2094
Bst4CI	ACNGT	3 cut(s)	876, 2023, 2401
Bst6I	CTCTTC	2 cut(s)	373, 540
BstACI	GRCGYC	1 cut(s)	868
BstAPI	GCANNNNNTGC	1 cut(s)	1307
BstAUI	TGTACA	1 cut(s)	1723
BstBAI	YACGTR	3 cut(s)	458, 1079, 2105
BstC8I	GCNNGC	2 cut(s)	2454, 2502
BstDEI	CTNAG	9 cut(s)	309, 351, 892, 960, 1139, 1449, 2073, 2807, 2844
BstDSI	CCRYGG	3 cut(s)	612, 2277, 3053
BstEII	GGTNACC	1 cut(s)	557
BstENI	CCTNNNNNAGG	1 cut(s)	627
BstFNI	CGCG	1 cut(s)	297
BstH2I	RGCGCY	1 cut(s)	33
BstHHI	GCGC	3 cut(s)	32, 2438, 2626
BstMAI	GTCTC	5 cut(s)	509, 797, 859, 976, 1790
BstMWI	GCNNNNNNNGC	7 cut(s)	835, 1307, 1445, 1769, 1903, 2435, 2458
BstNI	CCWGG	2 cut(s)	537, 2094
BstNSI	RCATGY	4 cut(s)	765, 1579, 1623, 3112
BstPI	GGTNACC	1 cut(s)	557
BstSCI	CCNGG	2 cut(s)	535, 2092
BstSFI	CTRYAG	3 cut(s)	1248, 1788, 2301
BstUI	CGCG	1 cut(s)	297
BstV1I	GCAGC	7 cut(s)	113, 292, 1075, 1262, 1297, 1730, 1857
BstV2I	GAAGAC	1 cut(s)	1402
BstX2I	RGATCY	3 cut(s)	388, 1690, 2416
BstXI	CCANNNNNNTGG	1 cut(s)	1975
BstYI	RGATCY	3 cut(s)	388, 1690, 2416
BstZ17I	GTATAC	1 cut(s)	3104
Bsu36I	CCTNAGG	1 cut(s)	960
BsuRI	GGCC	4 cut(s)	5, 706, 1460, 1532
BtgI	CCRYGG	3 cut(s)	612, 2277, 3053
BtgZI	GCGATG	1 cut(s)	2112
BtrI	CACGTC	1 cut(s)	858
BtsIMutI	CAGTG	4 cut(s)	13, 186, 1341, 2397
BveI	ACCTGC	1 cut(s)	815
Cac8I	GCNNGC	2 cut(s)	2454, 2502
CaiI	CAGNNNCTG	2 cut(s)	1694, 2798
CciI	TCATGA	3 cut(s)	1701, 2314, 2629
CfoI	GCGC	3 cut(s)	32, 2438, 2626
Cfr10I	RCCGGY	1 cut(s)	2500
Cfr13I	GGNCC	6 cut(s)	578, 608, 704, 922, 956, 2174
CseI	GACGC	1 cut(s)	239
DdeI	CTNAG	9 cut(s)	309, 351, 892, 960, 1139, 1449, 2073, 2807, 2844
DraI	TTTAAA	3 cut(s)	667, 1353, 2112
DraIII	CACNNNGTG	1 cut(s)	2401
DrdI	GACNNNNNNGTC	1 cut(s)	3074
DseDI	GACNNNNNNGTC	1 cut(s)	3074
EaeI	YGGCCR	2 cut(s)	3, 1530
Eam1104I	CTCTTC	2 cut(s)	373, 540
EarI	CTCTTC	2 cut(s)	373, 540
Ecl136II	GAGCTC	1 cut(s)	2597
Eco130I	CCWWGG	5 cut(s)	612, 1678, 2277, 2529, 3053
Eco24I	GRGCYC	1 cut(s)	2599
Eco31I	GGTCTC	1 cut(s)	797
Eco32I	GATATC	2 cut(s)	658, 2224
Eco47I	GGWCC	5 cut(s)	578, 608, 922, 956, 2174
Eco53kI	GAGCTC	1 cut(s)	2597
Eco57I	CTGAAG	5 cut(s)	354, 668, 804, 1047, 2952
Eco72I	CACGTG	1 cut(s)	458
Eco81I	CCTNAGG	1 cut(s)	960
Eco88I	CYCGRG	2 cut(s)	598, 627
Eco91I	GGTNACC	1 cut(s)	557
EcoICRI	GAGCTC	1 cut(s)	2597
EcoNI	CCTNNNNNAGG	1 cut(s)	627
EcoO109I	RGGNCCY	1 cut(s)	608
EcoO65I	GGTNACC	1 cut(s)	557
EcoRI	GAATTC	2 cut(s)	43, 1210
EcoRII	CCWGG	2 cut(s)	535, 2092
EcoRV	GATATC	2 cut(s)	658, 2224
EcoT14I	CCWWGG	5 cut(s)	612, 1678, 2277, 2529, 3053
EcoT38I	GRGCYC	1 cut(s)	2599
ErhI	CCWWGG	5 cut(s)	612, 1678, 2277, 2529, 3053
Esp3I	CGTCTC	1 cut(s)	859
FalI	AAGNNNNNCTT	4 cut(s)	73, 105, 546, 578
FaqI	GGGAC	5 cut(s)	564, 594, 1254, 1495, 2255
FauI	CCCGC	1 cut(s)	2844
FauNDI	CATATG	3 cut(s)	382, 652, 2142
FbaI	TGATCA	1 cut(s)	421
FblI	GTMKAC	1 cut(s)	3103
Fnu4HI	GCNGC	7 cut(s)	127, 306, 1089, 1251, 1311, 1719, 1871
FriOI	GRGCYC	1 cut(s)	2599
Fsp4HI	GCNGC	7 cut(s)	127, 306, 1089, 1251, 1311, 1719, 1871
FspBI	CTAG	7 cut(s)	12, 209, 779, 1898, 1958, 2901, 3119
GlaI	GCGC	3 cut(s)	31, 2437, 2625
GluI	GCNGC	7 cut(s)	127, 306, 1089, 1251, 1311, 1719, 1871
GsaI	CCCAGC	1 cut(s)	2294
GsuI	CTGGAG	1 cut(s)	257
HaeII	RGCGCY	1 cut(s)	33
HaeIII	GGCC	4 cut(s)	5, 706, 1460, 1532
HapII	CCGG	2 cut(s)	2501, 2940
HgaI	GACGC	1 cut(s)	239
HhaI	GCGC	3 cut(s)	32, 2438, 2626
Hin1I	GRCGYC	1 cut(s)	868
Hin6I	GCGC	3 cut(s)	30, 2436, 2624
HinP1I	GCGC	3 cut(s)	30, 2436, 2624
HincII	GTYRAC	2 cut(s)	1036, 2479
HindII	GTYRAC	2 cut(s)	1036, 2479
HindIII	AAGCTT	2 cut(s)	484, 2964
HpaII	CCGG	2 cut(s)	2501, 2940
HphI	GGTGA	3 cut(s)	1172, 1997, 2623
Hpy166II	GTNNAC	8 cut(s)	293, 872, 983, 1036, 1341, 2353, 2479, 3104
Hpy8I	GTNNAC	8 cut(s)	293, 872, 983, 1036, 1341, 2353, 2479, 3104
Hpy99I	CGWCG	1 cut(s)	1287
HpyAV	CCTTC	9 cut(s)	548, 828, 1807, 1864, 2053, 2608, 2659, 2887, 2914
HpyCH4III	ACNGT	3 cut(s)	876, 2023, 2401
HpyCH4IV	ACGT	7 cut(s)	457, 857, 868, 1078, 1611, 2104, 2871
HpyF10VI	GCNNNNNNNGC	7 cut(s)	835, 1307, 1445, 1769, 1903, 2435, 2458
HpyF3I	CTNAG	9 cut(s)	309, 351, 892, 960, 1139, 1449, 2073, 2807, 2844
HpySE526I	ACGT	7 cut(s)	457, 857, 868, 1078, 1611, 2104, 2871
Hsp92I	GRCGYC	1 cut(s)	868
HspAI	GCGC	3 cut(s)	30, 2436, 2624
KflI	GGGWCCC	1 cut(s)	608
Kpn2I	TCCGGA	1 cut(s)	2939
KpnI	GGTACC	1 cut(s)	2980
KroI	GCCGGC	1 cut(s)	2500
KroNI	GCCGGC	1 cut(s)	2502
Ksp22I	TGATCA	1 cut(s)	421
LguI	GCTCTTC	1 cut(s)	373
LmnI	GCTCC	5 cut(s)	335, 490, 1572, 2594, 2942
Lsp1109I	GCAGC	7 cut(s)	113, 292, 1075, 1262, 1297, 1730, 1857
LweI	GCATC	7 cut(s)	295, 322, 1857, 1895, 2039, 2713, 2845
MaeI	CTAG	7 cut(s)	12, 209, 779, 1898, 1958, 2901, 3119
MaeII	ACGT	7 cut(s)	457, 857, 868, 1078, 1611, 2104, 2871
MaeIII	GTNAC	5 cut(s)	557, 2009, 2320, 2983, 3032
MfeI	CAATTG	4 cut(s)	1199, 1730, 2325, 2469
MflI	RGATCY	3 cut(s)	388, 1690, 2416
MhlI	GDGCHC	2 cut(s)	340, 2599
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	5 cut(s)	407, 610, 1477, 2675, 2870
MmeI	TCCRAC	3 cut(s)	532, 1339, 1565
Mox20I	TGGCCA	1 cut(s)	5
MroI	TCCGGA	1 cut(s)	2939
MroNI	GCCGGC	1 cut(s)	2500
MroXI	GAANNNNTTC	3 cut(s)	531, 1380, 2253
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	7 cut(s)	482, 666, 831, 1352, 1476, 2111, 2423
MslI	CAYNNNNRTG	4 cut(s)	1862, 2051, 2190, 2523
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	1721
MspI	CCGG	2 cut(s)	2501, 2940
MspR9I	CCNGG	2 cut(s)	537, 2094
MunI	CAATTG	4 cut(s)	1199, 1730, 2325, 2469
Mva1269I	GAATGC	2 cut(s)	1184, 1741
MvaI	CCWGG	2 cut(s)	537, 2094
MvnI	CGCG	1 cut(s)	297
MwoI	GCNNNNNNNGC	7 cut(s)	835, 1307, 1445, 1769, 1903, 2435, 2458
NaeI	GCCGGC	1 cut(s)	2502
NcoI	CCATGG	3 cut(s)	612, 2277, 3053
NdeI	CATATG	3 cut(s)	382, 652, 2142
NgoMIV	GCCGGC	1 cut(s)	2500
NlaIV	GGNNCC	6 cut(s)	580, 609, 610, 923, 1686, 2978
NmuCI	GTSAC	3 cut(s)	2320, 2983, 3032
NspI	RCATGY	4 cut(s)	765, 1579, 1623, 3112
PaeR7I	CTCGAG	1 cut(s)	627
PagI	TCATGA	3 cut(s)	1701, 2314, 2629
PciSI	GCTCTTC	1 cut(s)	373
PctI	GAATGC	2 cut(s)	1184, 1741
PdiI	GCCGGC	1 cut(s)	2502
PdmI	GAANNNNTTC	3 cut(s)	531, 1380, 2253
PflMI	CCANNNNNTGG	1 cut(s)	2492
PkrI	GCNGC	7 cut(s)	128, 307, 1090, 1252, 1312, 1720, 1872
PleI	GAGTC	5 cut(s)	407, 609, 1477, 2675, 2869
PmaCI	CACGTG	1 cut(s)	458
PmlI	CACGTG	1 cut(s)	458
PpsI	GAGTC	5 cut(s)	407, 609, 1477, 2675, 2869
Ppu21I	YACGTR	3 cut(s)	458, 1079, 2105
PpuMI	RGGWCCY	1 cut(s)	608
PsiI	TTATAA	2 cut(s)	729, 1836
Psp124BI	GAGCTC	1 cut(s)	2599
Psp5II	RGGWCCY	1 cut(s)	608
Psp6I	CCWGG	2 cut(s)	535, 2092
PspCI	CACGTG	1 cut(s)	458
PspEI	GGTNACC	1 cut(s)	557
PspFI	CCCAGC	1 cut(s)	2290
PspGI	CCWGG	2 cut(s)	535, 2092
PspN4I	GGNNCC	6 cut(s)	580, 609, 610, 923, 1686, 2978
PspPI	GGNCC	6 cut(s)	578, 608, 704, 922, 956, 2174
PspPPI	RGGWCCY	1 cut(s)	608
PspXI	VCTCGAGB	1 cut(s)	627
PstI	CTGCAG	2 cut(s)	1252, 1792
PstNI	CAGNNNCTG	2 cut(s)	1694, 2798
PsuI	RGATCY	3 cut(s)	388, 1690, 2416
PvuII	CAGCTG	1 cut(s)	1721
RseI	CAYNNNNRTG	4 cut(s)	1862, 2051, 2190, 2523
SacI	GAGCTC	1 cut(s)	2599
SapI	GCTCTTC	1 cut(s)	373
SaqAI	TTAA	7 cut(s)	482, 666, 831, 1352, 1476, 2111, 2423
SatI	GCNGC	7 cut(s)	127, 306, 1089, 1251, 1311, 1719, 1871
Sau96I	GGNCC	6 cut(s)	578, 608, 704, 922, 956, 2174
SchI	GAGTC	5 cut(s)	407, 610, 1477, 2675, 2870
ScrFI	CCNGG	2 cut(s)	537, 2094
SduI	GDGCHC	2 cut(s)	340, 2599
SfaNI	GCATC	7 cut(s)	295, 322, 1857, 1895, 2039, 2713, 2845
SfcI	CTRYAG	3 cut(s)	1248, 1788, 2301
Sfr274I	CTCGAG	1 cut(s)	627
SinI	GGWCC	5 cut(s)	578, 608, 922, 956, 2174
SlaI	CTCGAG	1 cut(s)	627
SmiMI	CAYNNNNRTG	4 cut(s)	1862, 2051, 2190, 2523
SmlI	CTYRAG	1 cut(s)	627
SmoI	CTYRAG	1 cut(s)	627
SpeI	ACTAGT	1 cut(s)	208
SsiI	CCGC	7 cut(s)	202, 297, 743, 1123, 1651, 1906, 2851
SspI	AATATT	1 cut(s)	2345
SspMI	CTAG	7 cut(s)	12, 209, 779, 1898, 1958, 2901, 3119
SstI	GAGCTC	1 cut(s)	2599
StyD4I	CCNGG	2 cut(s)	535, 2092
StyI	CCWWGG	5 cut(s)	612, 1678, 2277, 2529, 3053
TaaI	ACNGT	3 cut(s)	876, 2023, 2401
TaiI	ACGT	7 cut(s)	460, 860, 871, 1081, 1614, 2107, 2874
TaqI	TCGA	7 cut(s)	249, 523, 628, 1051, 1173, 1504, 2271
TatI	WGTACW	4 cut(s)	21, 840, 982, 1723
Tru1I	TTAA	7 cut(s)	482, 666, 831, 1352, 1476, 2111, 2423
Tru9I	TTAA	7 cut(s)	482, 666, 831, 1352, 1476, 2111, 2423
TscAI	CASTG	4 cut(s)	13, 186, 1348, 2404
TseFI	GTSAC	3 cut(s)	2320, 2983, 3032
TseI	GCWGC	7 cut(s)	126, 305, 1088, 1250, 1310, 1718, 1870
Tsp45I	GTSAC	3 cut(s)	2320, 2983, 3032
TspGWI	ACGGA	1 cut(s)	348
TspRI	CASTG	4 cut(s)	13, 186, 1348, 2404
Van91I	CCANNNNNTGG	1 cut(s)	2492
VpaK11BI	GGWCC	5 cut(s)	578, 608, 922, 956, 2174
XagI	CCTNNNNNAGG	1 cut(s)	627
XapI	RAATTY	3 cut(s)	43, 1169, 1210
XbaI	TCTAGA	2 cut(s)	1957, 3118
XceI	RCATGY	4 cut(s)	765, 1579, 1623, 3112
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3134
XhoI	CTCGAG	1 cut(s)	627
XmiI	GTMKAC	1 cut(s)	3103
XmnI	GAANNNNTTC	3 cut(s)	531, 1380, 2253
XspI	CTAG	7 cut(s)	12, 209, 779, 1898, 1958, 2901, 3119
ZraI	GACGTC	1 cut(s)	869

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.