Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Forward (+)

3342681 .. 3347256

Gene Structure 4576 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005990

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3519 bp

ATGGGGAAGACAATAAAGTTACATGGATTCCCTTCAGGTGAATCTGCCGAAAAAATTAAGGTGTTTTTGGAGAAACATAGAGGAGAAGGAAGTGTTTTTGCTCTTAAATTACGGCCTGCCCAAAGGGGGGGGGGAAGATTCTATGCTTATATACAGTTTGAGTCCACCAGTCAGGCTGAGCTTATCACCTCTTTGGCTAACGCTAGGGGTAGGCTGTGGTATGGGAGATCATATTTGAAAGCTCATCCAGCGGACCATGATATTGTACCAAAGCCAAGAACTGTAATGCATAGCATGCAAAATTTAGTACTGCATGTTGGCTGCCAGGTTGCAAATGATAAGTTGTCTGTTTTTTGGAGAACGGCTGAAGTTTCAGTGGAGTTCGGGACTGGAACGCGGACATTCCAATTTCGGCTGTTATATCATCAGAAGGTATACAGACTTCAGCTTTCGTATGAGAACATCTGGCAAATTGAGTTGCATCGTCCACGTGGTCGTACTGCGAAGTATCTTGTGACTCAGTTATATGGAGCTCCTCGCATCTACCAACAAGAAGAAGCATGTTCTTCCGGAAATGTGTATGAAGACCCTCTTATGAACTACTTTAGGGATGTCCCTGATGACCAATGGATTATGACTACTGATTTCATTCCACGAAGCTGCATTGGGGAGTCATCTTCATTATGTTTAGAGCTTCCTTCTGGTCGCCAACTTCCTGATTTTGAAGAAAATTTTCCTTATTATAAGGAGAGTGATGGGCGATTTAATTTGGAGAATGGCTCAACTTTCTCTTGCAACCGAGATCTGGTCCCTATTATTGGTTCTCCTGCAGGGGTTGATTTGCCATATGATATCTTGTTTAAAATAAACTCGCTGGTTCAAAGGGGTTGTCTATCAGGGCCGACACTTGATAATAGGTTTTATAGGCTGGTGGATCCACGAAGAACAACTCGTTCCTGTATAGAGTATGCCCTAGATAAATTATCTAATTTGAAAGAGAGCTGTTATGAACCTGCGGTGTGGCTTCGTGAGGAATATCGGAAGCACCGGACATCAAAGAAACCTCCAAAGCCGGCACTTATCTCCTTAGATTCTGGTTTGGTATATGTACGCCGGGTTCAAATAACACCATCTAAAGTGTATTTCTCTGGTCCTGAGGTTAATGTCTCAAATCGCATACTACGCCAGTATTCCAGTGATATTGATAACTTTCTTCGTGTTTCATTTGTTGATGAGGAGATGGAAAAACTCTATTCAACAGATTTATCCTCCCGTACATCTACTAGGAAAACTCGCATCTACAACAGAGTTCTTTCTGTTCTCAGAAACGGCATAGTAATTGGAGAAAAGAAATTTGAGTTTCTTGCATTCTCATCAAGTCAATTGAAAGAGAATTCATTGTGGATGTTTGCTTCAACCTATACAGTTAGTGCTGATAAGATAAGAGCAGGGATGGGGGACTTTCGCAAGATAAGAAATGTGGCTAAGTATGCTGCCAGATTGGGTCAATCTTTTGGCTCTTCTACTGAAACTCTAAGTGTTGGCAGGCATGAAATTGAAGTTATTCCTGATGTAACCGTTAACTGGGGTGGAGTCAAATATGTGTTCTCTGATGGGATTGGGAAAATATCAGCTGATTTTGCAAGGAGAGTAGCCTTCAAATGTGGCTATAAAGCGTTCACACCATCTGCATTTCAAATTCGATATGGTGGTTACAAAGGTGTTGTAGCTGTTGATCCAACCTCATCAGTGAAATTATCACTGAGAAAGAGCATGTCCAAGTTTGATTCTGAAAACACAAAGCTAGATGTTCTAGCATTTAGCAAGTTTCAGCCTTGTTTTTTGAATCGTCAGGTGATAACTCTTCTGTCTACACTCGAAGTTCAAGATCATGTTTTCATGAAAAAGCAAAGAGCAGCTGTACAACAACTTAATGCCATATTGACTGACCCTTTAAAAGCAGAGGAGGCTCTTGATTTGATGTCTGCGGGTGAGAGTACAAACATCCTCAAGGAACTGCTCATGTGTGGTTATAAACCTGATGTTGAACCATTCCTTTCAATGATGCTCCAAACATTTCGGGCATCAAAGTTGTTGGAATTGAGGACCAAAACAAGAATCCTTATCCCAGATGGAAGAGCAATGATGGGATGTCTAGACGAAACAAGAACCTTGGAATATGGGCAGGTGTTTGTGCAAATCTCTGGAAACAGACGAAGGCAGTTATTTGATGATTCATTCATGCATAGTGGAAGCTCATCAGGCAACTGTTTTGTTATTGAGGAAAAGGTGGTTGTTGCAAAGAACCCATGTTTACACCCTGGTGATGTGCGTGTTTTAAGGGCTGTGAATGTGCCGGAGTTACACCATATGGTGGACTGTGTGGTATTTCCACAAAAAGGACCAAGGCCTCATCCAAATGAATGTTCTGGAAGTGATCTTGATGGAGATATTTACTTTGTTTGCTGGGACCCCGAGTTGATTCCTCGCAAGCAAATCCAACCTATGGATTATACCGCAGCACCAACTATAGAGATGGATCATGATGTCACAATTGAGGAAGTAGAAGAGTACTTTGTGAATTACAAACTCAATGATAGTTTGGGGATAATTGCCAGTGCCCACACTGTTTTTGCTGACAGAGAGCGTGGTAAAGCAATGAGCCGTCCGTGCATAGAGCTCGCGAAGCTACATTCAAAAGCTGTAGACTTTCCAAAAACTGGCGTGCCAGCTGTGGTACCACCAGAACTGTATGTCAAGGAATATCCAGATTTCATGGAGAAGACTGACAAAAGCACCTATCTCTCAAGCAATGTGATCGGACAGCTTTTTCGTGAGGTGAAGGACATTGCACCCCATGGCAGCTGTATTACATCCTTCACTCATCAAGTGGCGACAAAATCCTATGACCCTGAAATGGAGGTTGATGGCTTTGAGGATTACCTTGAAGATGCTTTCTATTACAAAAACAATTATGATTACAAATTGGGAAACCTGTTGGATTACTACGGGATCAAGACAGAGGCTGAAATATTGAGTGGGGGTATTATGAGAATGTCAAAATCTTTCACCAAGAAGGGGGCTGCAGACGCTATAAACAGGGCTGTGAGGTCATTGAGGAAGGAAGCTAGGACCTGGTTCGATGAGAAAGGAACTGGAATAGATTCTGGAGTTGATGATGTCTATGCAAAAGCTTCAGCTTGGTACCATGTCACATATCATCCCAATTATTTTGGTTTGTACAATGAGGGAATGAAACGTGACCATTTTATCAGCTTCCCGTGGTGTGTGTATGACAAGCTAGTTCACATAAAAAAAGGACACAGTAAAGCAAGCTCGGCGCAGATCATCTCTAGAAAGTCAGTTCAGGTACGGCTTGCATTTGTTCTGATGAAGTTGGCAGAATTGAACTTTGTTGATGGTGCTGATGATCAAGACTTGAAGGCAATTTTCTTGTATAATATCCACCTTTGGTTTGTTTTTCATTTGTTGTATAATGTACACAAACCGCTGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

1173

Amino Acids

133.46

Weight (kDa)

8.62

Isoelectric Point (pI)

36.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_RdRP1_2	PF26250	4 - 85	2.8e-16	RdRP1/2, RRM domain
PH_RDR2	PF24823	104 - 255	6.3e-41	RDR2 PH-like domain
RdRP_helical	PF26252	278 - 354	8.9e-13	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	375 - 947	1.3e-194	RNA dependent RNA polymerase
RdRP_head	PF26253	968 - 1115	5.8e-47	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	744, 2034
AarI	CACCTGC	1 cut(s)	2176
Acc36I	ACCTGC	2 cut(s)	1021, 2176
Acc65I	GGTACC	2 cut(s)	2737, 3204
AccB1I	GGYRCC	2 cut(s)	2737, 3204
AccB7I	CCANNNNNTGG	3 cut(s)	2374, 2506, 2720
AccI	GTMKAC	3 cut(s)	435, 1871, 2706
AccII	CGCG	2 cut(s)	397, 2684
AccIII	TCCGGA	1 cut(s)	569
AciI	CCGC	6 cut(s)	251, 397, 1016, 1988, 2517, 3509
AclWI	GGATC	5 cut(s)	929, 942, 1730, 2547, 3020
AcsI	RAATTY	5 cut(s)	301, 730, 1352, 1393, 1698
AcuI	CTGAAG	4 cut(s)	18, 387, 428, 3180
AcvI	CACGTG	1 cut(s)	491
AjnI	CCWGG	3 cut(s)	324, 2320, 3134
AjuI	GAANNNNNNNTTGG	2 cut(s)	2410, 2442
AleI	CACNNNNGTG	1 cut(s)	2322
Alw21I	GWGCWC	2 cut(s)	535, 2682
Alw26I	GTCTC	1 cut(s)	1171
AlwI	GGATC	5 cut(s)	929, 942, 1730, 2547, 3020
AlwNI	CAGNNNCTG	1 cut(s)	3026
Ama87I	CYCGRG	1 cut(s)	2474
Aor13HI	TCCGGA	1 cut(s)	569
AoxI	GGCC	3 cut(s)	113, 899, 2408
ApeKI	GCWGC	7 cut(s)	321, 660, 1493, 1916, 2519, 2862, 3083
ApoI	RAATTY	5 cut(s)	301, 730, 1352, 1393, 1698
ArsI	GACNNNNNNTTYG	4 cut(s)	874, 906, 2814, 2846
Asp700I	GAANNNNTTC	3 cut(s)	732, 1563, 3163
Asp718I	GGTACC	2 cut(s)	2737, 3204
AspLEI	GCGC	1 cut(s)	3343
AspS9I	GGNCC	8 cut(s)	253, 808, 899, 1151, 2106, 2402, 2470, 3132
AsuC2I	CCSGG	1 cut(s)	1115
AsuHPI	GGTGA	7 cut(s)	50, 178, 1867, 2003, 2336, 2851, 3061
AvaI	CYCGRG	1 cut(s)	2474
AvaII	GGWCC	7 cut(s)	253, 808, 1151, 2106, 2402, 2470, 3132
AxyI	CCTNAGG	1 cut(s)	1155
BaeGI	GKGCMC	1 cut(s)	2623
BamHI	GGATCC	1 cut(s)	934
BanI	GGYRCC	2 cut(s)	2737, 3204
BanII	GRGCYC	2 cut(s)	535, 2682
BarI	GAAGNNNNNNTAC	2 cut(s)	426, 458
BbrPI	CACGTG	1 cut(s)	491
BbsI	GAAGAC	3 cut(s)	14, 591, 2789
Bbv12I	GWGCWC	2 cut(s)	535, 2682
BbvI	GCAGC	7 cut(s)	308, 647, 1480, 1928, 2531, 2874, 3070
BceAI	ACGGC	5 cut(s)	128, 378, 1345, 2649, 3389
BcgI	CGANNNNNNTGC	4 cut(s)	2662, 2696, 2800, 2834
BciT130I	CCWGG	3 cut(s)	326, 2322, 3136
BclI	TGATCA	1 cut(s)	3430
BcnI	CCSGG	1 cut(s)	1115
BcoDI	GTCTC	1 cut(s)	1171
BfaI	CTAG	9 cut(s)	204, 974, 1284, 1805, 1814, 2156, 3129, 3302, 3354
BfmI	CTRYAG	4 cut(s)	828, 2529, 2703, 3084
BfuAI	ACCTGC	2 cut(s)	1021, 2176
BglII	AGATCT	1 cut(s)	802
BisI	GCNGC	7 cut(s)	322, 661, 1494, 1917, 2520, 2863, 3084
BlpI	GCTNAGC	1 cut(s)	177
BlsI	GCNGC	7 cut(s)	323, 662, 1495, 1918, 2521, 2864, 3085
BmcAI	AGTACT	2 cut(s)	309, 2573
Bme1390I	CCNGG	4 cut(s)	326, 1115, 2322, 3136
Bme18I	GGWCC	7 cut(s)	253, 808, 1151, 2106, 2402, 2470, 3132
BmeT110I	CYCGRG	1 cut(s)	2474
BmgT120I	GGNCC	8 cut(s)	253, 808, 899, 1151, 2106, 2402, 2470, 3132
BmiI	GGNNCC	6 cut(s)	810, 936, 2471, 2472, 2739, 3206
BmrFI	CCNGG	4 cut(s)	326, 1115, 2322, 3136
BmrI	ACTGGG	1 cut(s)	1594
BmsI	GCATC	6 cut(s)	490, 549, 1305, 2055, 2093, 2941
BmuI	ACTGGG	1 cut(s)	1594
BpiI	GAAGAC	3 cut(s)	14, 591, 2789
BplI	GAGNNNNNCTC	2 cut(s)	764, 796
BpmI	CTGGAG	1 cut(s)	3189
Bpu1102I	GCTNAGC	1 cut(s)	177
BpuEI	CTTGAG	2 cut(s)	1994, 2791
BpuMI	CCSGG	1 cut(s)	1115
BsaAI	YACGTR	1 cut(s)	491
BsaJI	CCNNGG	5 cut(s)	2172, 2320, 2405, 2857, 3281
BsaWI	WCCGGW	2 cut(s)	569, 1047
Bse118I	RCCGGY	1 cut(s)	1072
Bse1I	ACTGG	8 cut(s)	168, 394, 1186, 1194, 1589, 2616, 2725, 3160
Bse21I	CCTNAGG	1 cut(s)	1155
Bse3DI	GCAATG	4 cut(s)	2148, 2664, 2818, 2847
BseAI	TCCGGA	1 cut(s)	569
BseBI	CCWGG	3 cut(s)	326, 2322, 3136
BseDI	CCNNGG	5 cut(s)	2172, 2320, 2405, 2857, 3281
BseGI	GGATG	9 cut(s)	244, 616, 1410, 1458, 2004, 2156, 2413, 2873, 3220
BseMI	GCAATG	4 cut(s)	2148, 2664, 2818, 2847
BseMII	CTCAG	5 cut(s)	168, 533, 1146, 1336, 1754
BseNI	ACTGG	8 cut(s)	168, 394, 1186, 1194, 1589, 2616, 2725, 3160
BseRI	GAGGAG	4 cut(s)	96, 525, 1250, 1979
BseSI	GKGCMC	1 cut(s)	2623
BseXI	GCAGC	7 cut(s)	308, 647, 1480, 1928, 2531, 2874, 3070
BseYI	CCCAGC	1 cut(s)	2466
Bsh1236I	CGCG	2 cut(s)	397, 2684
BshFI	GGCC	3 cut(s)	115, 901, 2410
BshNI	GGYRCC	2 cut(s)	2737, 3204
BsiHKAI	GWGCWC	2 cut(s)	535, 2682
BsiHKCI	CYCGRG	1 cut(s)	2474
BsiSI	CCGG	5 cut(s)	570, 1048, 1073, 1114, 2357
BslFI	GGGAC	5 cut(s)	400, 599, 794, 1472, 2483
BsmAI	GTCTC	1 cut(s)	1171
BsmFI	GGGAC	5 cut(s)	400, 599, 794, 1472, 2483
BsmI	GAATGC	1 cut(s)	1367
BsnI	GGCC	3 cut(s)	115, 901, 2410
BsoBI	CYCGRG	1 cut(s)	2474
Bsp1286I	GDGCHC	3 cut(s)	535, 2623, 2682
Bsp13I	TCCGGA	1 cut(s)	569
Bsp1407I	TGTACA	3 cut(s)	1921, 3240, 3499
Bsp1720I	GCTNAGC	1 cut(s)	177
Bsp19I	CCATGG	1 cut(s)	2857
Bsp68I	TCGCGA	1 cut(s)	2684
BspACI	CCGC	6 cut(s)	251, 397, 1016, 1988, 2517, 3509
BspANI	GGCC	3 cut(s)	115, 901, 2410
BspCNI	CTCAG	5 cut(s)	169, 532, 1147, 1335, 1755
BspEI	TCCGGA	1 cut(s)	569
BspFNI	CGCG	2 cut(s)	397, 2684
BspHI	TCATGA	2 cut(s)	1899, 2542
BspLI	GGNNCC	6 cut(s)	810, 936, 2471, 2472, 2739, 3206
BspMAI	CTGCAG	2 cut(s)	832, 3088
BspMI	ACCTGC	2 cut(s)	1021, 2176
BspPI	GGATC	5 cut(s)	929, 942, 1730, 2547, 3020
BspQI	GCTCTTC	2 cut(s)	1525, 2131
BspT107I	GGYRCC	2 cut(s)	2737, 3204
BsrDI	GCAATG	4 cut(s)	2148, 2664, 2818, 2847
BsrFI	RCCGGY	1 cut(s)	1072
BsrGI	TGTACA	3 cut(s)	1921, 3240, 3499
BsrI	ACTGG	8 cut(s)	168, 394, 1186, 1194, 1589, 2616, 2725, 3160
BssAI	RCCGGY	1 cut(s)	1072
BssECI	CCNNGG	5 cut(s)	2172, 2320, 2405, 2857, 3281
BssNAI	GTATAC	1 cut(s)	436
BssT1I	CCWWGG	3 cut(s)	2172, 2405, 2857
Bst1107I	GTATAC	1 cut(s)	436
Bst2UI	CCWGG	3 cut(s)	326, 2322, 3136
Bst4CI	ACNGT	9 cut(s)	156, 283, 1426, 1579, 2270, 2381, 2629, 2751, 3326
Bst6I	CTCTTC	4 cut(s)	1525, 1869, 2131, 2562
BstAPI	GCANNNNNTGC	1 cut(s)	295
BstAUI	TGTACA	3 cut(s)	1921, 3240, 3499
BstBAI	YACGTR	1 cut(s)	491
BstDEI	CTNAG	8 cut(s)	177, 519, 1087, 1155, 1322, 1485, 1535, 1763
BstDSI	CCRYGG	2 cut(s)	2857, 3281
BstF5I	GGATG	9 cut(s)	244, 616, 1410, 1458, 2004, 2156, 2413, 2873, 3220
BstFNI	CGCG	2 cut(s)	397, 2684
BstHHI	GCGC	1 cut(s)	3343
BstMAI	GTCTC	1 cut(s)	1171
BstNI	CCWGG	3 cut(s)	326, 2322, 3136
BstNSI	RCATGY	4 cut(s)	298, 317, 564, 1777
BstSCI	CCNGG	4 cut(s)	324, 1113, 2320, 3134
BstSFI	CTRYAG	4 cut(s)	828, 2529, 2703, 3084
BstSLI	GKGCMC	1 cut(s)	2623
BstUI	CGCG	2 cut(s)	397, 2684
BstV1I	GCAGC	7 cut(s)	308, 647, 1480, 1928, 2531, 2874, 3070
BstV2I	GAAGAC	3 cut(s)	14, 591, 2789
BstX2I	RGATCY	2 cut(s)	802, 934
BstYI	RGATCY	2 cut(s)	802, 934
BstZ17I	GTATAC	1 cut(s)	436
Bsu36I	CCTNAGG	1 cut(s)	1155
BsuRI	GGCC	3 cut(s)	115, 901, 2410
BtgI	CCRYGG	2 cut(s)	2857, 3281
BtsCI	GGATG	9 cut(s)	244, 616, 1410, 1458, 2004, 2156, 2413, 2873, 3220
BtsIMutI	CAGTG	6 cut(s)	381, 1201, 1755, 1760, 2623, 2625
BtuMI	TCGCGA	1 cut(s)	2684
BveI	ACCTGC	2 cut(s)	1021, 2176
CaiI	CAGNNNCTG	1 cut(s)	3026
CciI	TCATGA	2 cut(s)	1899, 2542
CfoI	GCGC	1 cut(s)	3343
Cfr10I	RCCGGY	1 cut(s)	1072
Cfr13I	GGNCC	8 cut(s)	253, 808, 899, 1151, 2106, 2402, 2470, 3132
CseI	GACGC	1 cut(s)	3098
CsiI	ACCWGGT	1 cut(s)	3134
DdeI	CTNAG	8 cut(s)	177, 519, 1087, 1155, 1322, 1485, 1535, 1763
DraI	TTTAAA	2 cut(s)	862, 1956
Eam1104I	CTCTTC	4 cut(s)	1525, 1869, 2131, 2562
EarI	CTCTTC	4 cut(s)	1525, 1869, 2131, 2562
Ecl136II	GAGCTC	2 cut(s)	533, 2680
Eco130I	CCWWGG	3 cut(s)	2172, 2405, 2857
Eco147I	AGGCCT	1 cut(s)	2410
Eco24I	GRGCYC	2 cut(s)	535, 2682
Eco32I	GATATC	1 cut(s)	853
Eco47I	GGWCC	7 cut(s)	253, 808, 1151, 2106, 2402, 2470, 3132
Eco53kI	GAGCTC	2 cut(s)	533, 2680
Eco57I	CTGAAG	4 cut(s)	18, 387, 428, 3180
Eco72I	CACGTG	1 cut(s)	491
Eco81I	CCTNAGG	1 cut(s)	1155
Eco88I	CYCGRG	1 cut(s)	2474
EcoICRI	GAGCTC	2 cut(s)	533, 2680
EcoO109I	RGGNCCY	2 cut(s)	2470, 3132
EcoRI	GAATTC	1 cut(s)	1393
EcoRII	CCWGG	3 cut(s)	324, 2320, 3134
EcoRV	GATATC	1 cut(s)	853
EcoT14I	CCWWGG	3 cut(s)	2172, 2405, 2857
EcoT22I	ATGCAT	2 cut(s)	291, 2247
EcoT38I	GRGCYC	2 cut(s)	535, 2682
ErhI	CCWWGG	3 cut(s)	2172, 2405, 2857
FalI	AAGNNNNNCTT	2 cut(s)	576, 608
FaqI	GGGAC	5 cut(s)	400, 599, 794, 1472, 2483
FauI	CCCGC	1 cut(s)	1981
FauNDI	CATATG	2 cut(s)	847, 2370
FbaI	TGATCA	1 cut(s)	3430
FblI	GTMKAC	3 cut(s)	435, 1871, 2706
Fnu4HI	GCNGC	7 cut(s)	322, 661, 1494, 1917, 2520, 2863, 3084
FokI	GGATG	9 cut(s)	231, 623, 1417, 1465, 1991, 2163, 2400, 2860, 3207
FriOI	GRGCYC	2 cut(s)	535, 2682
Fsp4HI	GCNGC	7 cut(s)	322, 661, 1494, 1917, 2520, 2863, 3084
FspBI	CTAG	9 cut(s)	204, 974, 1284, 1805, 1814, 2156, 3129, 3302, 3354
GlaI	GCGC	1 cut(s)	3342
GluI	GCNGC	7 cut(s)	322, 661, 1494, 1917, 2520, 2863, 3084
GsaI	CCCAGC	1 cut(s)	2470
GsuI	CTGGAG	1 cut(s)	3189
HaeIII	GGCC	3 cut(s)	115, 901, 2410
HapII	CCGG	5 cut(s)	570, 1048, 1073, 1114, 2357
HgaI	GACGC	1 cut(s)	3098
HhaI	GCGC	1 cut(s)	3343
Hin6I	GCGC	1 cut(s)	3341
HinP1I	GCGC	1 cut(s)	3341
HincII	GTYRAC	1 cut(s)	1582
HindII	GTYRAC	1 cut(s)	1582
HindIII	AAGCTT	1 cut(s)	3192
HpaI	GTTAAC	1 cut(s)	1582
HpaII	CCGG	5 cut(s)	570, 1048, 1073, 1114, 2357
HphI	GGTGA	7 cut(s)	50, 178, 1867, 2003, 2336, 2851, 3061
Hpy188I	TCNGA	7 cut(s)	429, 1041, 1325, 1612, 1792, 2822, 3390
HpyCH4III	ACNGT	9 cut(s)	156, 283, 1426, 1579, 2270, 2381, 2629, 2751, 3326
HpyCH4IV	ACGT	2 cut(s)	490, 3259
HpyF3I	CTNAG	8 cut(s)	177, 519, 1087, 1155, 1322, 1485, 1535, 1763
HpySE526I	ACGT	2 cut(s)	490, 3259
HspAI	GCGC	1 cut(s)	3341
KflI	GGGWCCC	1 cut(s)	2470
Kpn2I	TCCGGA	1 cut(s)	569
KpnI	GGTACC	2 cut(s)	2741, 3208
KroI	GCCGGC	1 cut(s)	1072
KroNI	GCCGGC	1 cut(s)	1074
Ksp22I	TGATCA	1 cut(s)	3430
KspAI	GTTAAC	1 cut(s)	1582
LguI	GCTCTTC	2 cut(s)	1525, 2131
LmnI	GCTCC	3 cut(s)	530, 538, 2073
Lsp1109I	GCAGC	7 cut(s)	308, 647, 1480, 1928, 2531, 2874, 3070
LweI	GCATC	6 cut(s)	490, 549, 1305, 2055, 2093, 2941
MabI	ACCWGGT	1 cut(s)	3134
MaeI	CTAG	9 cut(s)	204, 974, 1284, 1805, 1814, 2156, 3129, 3302, 3354
MaeII	ACGT	2 cut(s)	490, 3259
MaeIII	GTNAC	8 cut(s)	18, 514, 1573, 1712, 2361, 2548, 3211, 3260
MfeI	CAATTG	2 cut(s)	1382, 2553
MflI	RGATCY	2 cut(s)	802, 934
MhlI	GDGCHC	3 cut(s)	535, 2623, 2682
MlyI	GAGTC	4 cut(s)	170, 511, 680, 1602
MmeI	TCCRAC	4 cut(s)	1763, 2076, 2524, 2979
Mph1103I	ATGCAT	2 cut(s)	291, 2247
MroI	TCCGGA	1 cut(s)	569
MroNI	GCCGGC	1 cut(s)	1072
MroXI	GAANNNNTTC	3 cut(s)	732, 1563, 3163
MseI	TTAA	9 cut(s)	57, 105, 765, 861, 1161, 1581, 1932, 1955, 2339
MslI	CAYNNNNRTG	2 cut(s)	2322, 2418
MspA1I	CMGCKG	6 cut(s)	251, 1634, 1919, 2732, 2865, 3511
MspI	CCGG	5 cut(s)	570, 1048, 1073, 1114, 2357
MspR9I	CCNGG	4 cut(s)	326, 1115, 2322, 3136
MunI	CAATTG	2 cut(s)	1382, 2553
Mva1269I	GAATGC	1 cut(s)	1367
MvaI	CCWGG	3 cut(s)	326, 2322, 3136
MvnI	CGCG	2 cut(s)	397, 2684
NaeI	GCCGGC	1 cut(s)	1074
NciI	CCSGG	1 cut(s)	1115
NcoI	CCATGG	1 cut(s)	2857
NdeI	CATATG	2 cut(s)	847, 2370
NgoMIV	GCCGGC	1 cut(s)	1072
NlaIV	GGNNCC	6 cut(s)	810, 936, 2471, 2472, 2739, 3206
NmeAIII	GCCGAG	1 cut(s)	3317
NmuCI	GTSAC	4 cut(s)	514, 2548, 3211, 3260
NruI	TCGCGA	1 cut(s)	2684
NsiI	ATGCAT	2 cut(s)	291, 2247
NspI	RCATGY	4 cut(s)	298, 317, 564, 1777
OliI	CACNNNNGTG	1 cut(s)	2322
PaeI	GCATGC	1 cut(s)	298
PagI	TCATGA	2 cut(s)	1899, 2542
PaqCI	CACCTGC	1 cut(s)	2176
PceI	AGGCCT	1 cut(s)	2410
PciSI	GCTCTTC	2 cut(s)	1525, 2131
PctI	GAATGC	1 cut(s)	1367
PdiI	GCCGGC	1 cut(s)	1074
PdmI	GAANNNNTTC	3 cut(s)	732, 1563, 3163
PflMI	CCANNNNNTGG	3 cut(s)	2374, 2506, 2720
PkrI	GCNGC	7 cut(s)	323, 662, 1495, 1918, 2521, 2864, 3085
PleI	GAGTC	4 cut(s)	169, 511, 679, 1601
PmaCI	CACGTG	1 cut(s)	491
PmlI	CACGTG	1 cut(s)	491
PpsI	GAGTC	4 cut(s)	169, 511, 679, 1601
Ppu21I	YACGTR	1 cut(s)	491
PpuMI	RGGWCCY	2 cut(s)	2470, 3132
PsiI	TTATAA	2 cut(s)	744, 2034
Psp124BI	GAGCTC	2 cut(s)	535, 2682
Psp5II	RGGWCCY	2 cut(s)	2470, 3132
Psp6I	CCWGG	3 cut(s)	324, 2320, 3134
PspCI	CACGTG	1 cut(s)	491
PspFI	CCCAGC	1 cut(s)	2466
PspGI	CCWGG	3 cut(s)	324, 2320, 3134
PspN4I	GGNNCC	6 cut(s)	810, 936, 2471, 2472, 2739, 3206
PspPI	GGNCC	8 cut(s)	253, 808, 899, 1151, 2106, 2402, 2470, 3132
PspPPI	RGGWCCY	2 cut(s)	2470, 3132
PsrI	GAACNNNNNNTAC	2 cut(s)	1101, 1133
PstI	CTGCAG	2 cut(s)	832, 3088
PstNI	CAGNNNCTG	1 cut(s)	3026
PsuI	RGATCY	2 cut(s)	802, 934
PvuII	CAGCTG	4 cut(s)	1634, 1919, 2732, 2865
RruI	TCGCGA	1 cut(s)	2684
RseI	CAYNNNNRTG	2 cut(s)	2322, 2418
SacI	GAGCTC	2 cut(s)	535, 2682
SapI	GCTCTTC	2 cut(s)	1525, 2131
SaqAI	TTAA	9 cut(s)	57, 105, 765, 861, 1161, 1581, 1932, 1955, 2339
SatI	GCNGC	7 cut(s)	322, 661, 1494, 1917, 2520, 2863, 3084
Sau96I	GGNCC	8 cut(s)	253, 808, 899, 1151, 2106, 2402, 2470, 3132
SbfI	CCTGCAGG	1 cut(s)	832
ScaI	AGTACT	2 cut(s)	309, 2573
SchI	GAGTC	4 cut(s)	170, 511, 680, 1602
ScrFI	CCNGG	4 cut(s)	326, 1115, 2322, 3136
SdaI	CCTGCAGG	1 cut(s)	832
SduI	GDGCHC	3 cut(s)	535, 2623, 2682
SexAI	ACCWGGT	1 cut(s)	3134
SfaNI	GCATC	6 cut(s)	490, 549, 1305, 2055, 2093, 2941
SfcI	CTRYAG	4 cut(s)	828, 2529, 2703, 3084
SinI	GGWCC	7 cut(s)	253, 808, 1151, 2106, 2402, 2470, 3132
SmiMI	CAYNNNNRTG	2 cut(s)	2322, 2418
SmlI	CTYRAG	2 cut(s)	2009, 2806
SmoI	CTYRAG	2 cut(s)	2009, 2806
SphI	GCATGC	1 cut(s)	298
Sse8387I	CCTGCAGG	1 cut(s)	832
SseBI	AGGCCT	1 cut(s)	2410
SsiI	CCGC	6 cut(s)	251, 397, 1016, 1988, 2517, 3509
SspI	AATATT	1 cut(s)	3033
SspMI	CTAG	9 cut(s)	204, 974, 1284, 1805, 1814, 2156, 3129, 3302, 3354
SstI	GAGCTC	2 cut(s)	535, 2682
StuI	AGGCCT	1 cut(s)	2410
StyD4I	CCNGG	4 cut(s)	324, 1113, 2320, 3134
StyI	CCWWGG	3 cut(s)	2172, 2405, 2857
TaaI	ACNGT	9 cut(s)	156, 283, 1426, 1579, 2270, 2381, 2629, 2751, 3326
TaiI	ACGT	2 cut(s)	493, 3262
TaqI	TCGA	3 cut(s)	1702, 1878, 3141
TatI	WGTACW	6 cut(s)	307, 1921, 1997, 2571, 3240, 3499
Tru1I	TTAA	9 cut(s)	57, 105, 765, 861, 1161, 1581, 1932, 1955, 2339
Tru9I	TTAA	9 cut(s)	57, 105, 765, 861, 1161, 1581, 1932, 1955, 2339
TscAI	CASTG	6 cut(s)	381, 1201, 1755, 1767, 2623, 2632
TseFI	GTSAC	4 cut(s)	514, 2548, 3211, 3260
TseI	GCWGC	7 cut(s)	321, 660, 1493, 1916, 2519, 2862, 3083
Tsp45I	GTSAC	4 cut(s)	514, 2548, 3211, 3260
TspGWI	ACGGA	1 cut(s)	2658
TspRI	CASTG	6 cut(s)	381, 1201, 1755, 1767, 2623, 2632
Van91I	CCANNNNNTGG	3 cut(s)	2374, 2506, 2720
VpaK11BI	GGWCC	7 cut(s)	253, 808, 1151, 2106, 2402, 2470, 3132
XapI	RAATTY	5 cut(s)	301, 730, 1352, 1393, 1698
XbaI	TCTAGA	2 cut(s)	2155, 3353
XceI	RCATGY	4 cut(s)	298, 317, 564, 1777
XmiI	GTMKAC	3 cut(s)	435, 1871, 2706
XmnI	GAANNNNTTC	3 cut(s)	732, 1563, 3163
XspI	CTAG	9 cut(s)	204, 974, 1284, 1805, 1814, 2156, 3129, 3302, 3354
ZrmI	AGTACT	2 cut(s)	309, 2573
Zsp2I	ATGCAT	2 cut(s)	291, 2247

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.