FvH4_4g36360

Protein TOM THREE HOMOLOG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006559
Physical Location & Seq
Forward (+)
33320982 .. 33326085
5104 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36360.t1

Sequence Viewer

Length: 882 bp
ATGGCGCGAGGCGGCGGCGTAGTGGAGTCGGCGGCGGCGGTGATAGCTTACAATCTCAAAGAGTCGTCGAGCTGGTGGCACGACATCAATGCCTCGACGCTTTGGCAGGATCGTATCTTCCACGGCCTCGCCGTCCTCTATGGCCTCGTCGCCGTCGTTGCTGTGGTTCAATTGGTTAGGATACAAGTGCGGGTGCCGGAGTACGGCTGGACCACGCAGAAGGTCTTCCACTTTCTCAATTTCGTTGTGAATGCAGTGAGAGCAGCAGTGTTCATTTTCCGCCGGCAAGTACAGAGGCTACATCCAAAGATTGTGCAACATGTGTTGCTTGATATGCCGAGCCTGGCTTTCTTCACGACTTACGCGCTTTTGGTTCTGTTCTGGGCGGAAATCTACTATCAGGCGCGTGCTGTTTCTACGGATGGACTGAGGCCGAGCTTCTTTACTATCAATGCTGCTGTTTATGTAGTTCAGATAACTATGTGGTTTATATTATGGTGGAAGCCTGTCCCAGTCGTGGTCATCTTATCTAAAGTGTTCTTTGCAGGTGTCTCTTTATTTGCAGCCCTTGGATTTCTTCTGTATGGTGGAAGGCTCTTTTTGATGTTGCAGAGATTTCCTGTGGAGTCAAAAGGAAGACGTAAGAAGCTGCAGGAGGTTGGCTACGTGACAACCATATGTTTTACCTGTTTCCTCATCAGATGCGTAATGATGTGCTTATGTGCATTTGACAAGGCAGCGGACCTTGATGTTTTGAATCACCCAGTTCTAAACTTGATATATTACTTGTTGGTGGAGATATTGCCTTCTTCTTTGGTTCTTTTCATTTTAAGAAAGTTGCCTCCAAAGCGTGGGATCACACAGTATCATCCTATCCGCTGA

Protein Analysis

294

Amino Acids

33.52

Weight (kDa)

9.85

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 22 - 293 2.9e-147 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 536
Acc36I ACCTGC 1 cut(s) 536
AccB1I GGYRCC 1 cut(s) 193
AccB7I CCANNNNNTGG 1 cut(s) 851
AccII CGCG 3 cut(s) 7, 365, 406
AclWI GGATC 2 cut(s) 117, 863
AfaI GTAC 2 cut(s) 203, 291
AfiI CCNNNNNNNGG 3 cut(s) 203, 517, 851
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 2 cut(s) 170, 757
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 4 cut(s) 47, 72, 438, 649
AluI AGCT 4 cut(s) 47, 72, 438, 649
Alw26I GTCTC 1 cut(s) 556
AlwI GGATC 2 cut(s) 117, 863
AoxI GGCC 3 cut(s) 124, 142, 431
ApeKI GCWGC 5 cut(s) 263, 455, 563, 649, 737
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 3 cut(s) 7, 367, 406
AspS9I GGNCC 2 cut(s) 210, 742
AsuHPI GGTGA 2 cut(s) 52, 752
AvaII GGWCC 2 cut(s) 210, 742
BanI GGYRCC 1 cut(s) 193
BbsI GAAGAC 2 cut(s) 217, 643
BbvI GCAGC 5 cut(s) 275, 442, 575, 636, 749
BccI CCATC 1 cut(s) 416
BceAI ACGGC 4 cut(s) 116, 137, 139, 220
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 1 cut(s) 344
BciVI GTATCC 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 556
BfmI CTRYAG 1 cut(s) 650
BfuAI ACCTGC 1 cut(s) 536
BfuI GTATCC 1 cut(s) 174
BisI GCNGC 9 cut(s) 13, 16, 33, 36, 264, 456, 564, 650, 738
BlsI GCNGC 9 cut(s) 14, 17, 34, 37, 265, 457, 565, 651, 739
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 2 cut(s) 210, 742
BmgT120I GGNCC 2 cut(s) 210, 742
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 1 cut(s) 344
BmrI ACTGGG 2 cut(s) 506, 758
BmsI GCATC 1 cut(s) 692
BmuI ACTGGG 2 cut(s) 506, 758
BpiI GAAGAC 2 cut(s) 217, 643
BsaAI YACGTR 1 cut(s) 667
BsaJI CCNNGG 2 cut(s) 121, 568
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 517, 851
Bse118I RCCGGY 1 cut(s) 282
Bse1I ACTGG 2 cut(s) 512, 764
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 2 cut(s) 121, 568
BseGI GGATG 3 cut(s) 301, 427, 868
BseLI CCNNNNNNNGG 3 cut(s) 203, 517, 851
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 2 cut(s) 512, 764
BseXI GCAGC 5 cut(s) 275, 442, 575, 636, 749
Bsh1236I CGCG 3 cut(s) 7, 365, 406
BshFI GGCC 3 cut(s) 126, 144, 433
BshNI GGYRCC 1 cut(s) 193
BsiSI CCGG 2 cut(s) 197, 283
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 3 cut(s) 203, 517, 851
BsmAI GTCTC 1 cut(s) 556
BsmFI GGGAC 1 cut(s) 494
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 3 cut(s) 126, 144, 433
Bsp143I GATC 2 cut(s) 109, 855
BspANI GGCC 3 cut(s) 126, 144, 433
BspCNI CTCAG 1 cut(s) 420
BspFNI CGCG 3 cut(s) 7, 365, 406
BspLI GGNNCC 1 cut(s) 195
BspMAI CTGCAG 1 cut(s) 654
BspMI ACCTGC 1 cut(s) 536
BspPI GGATC 2 cut(s) 117, 863
BspT107I GGYRCC 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 282
BsrI ACTGG 2 cut(s) 512, 764
BssAI RCCGGY 1 cut(s) 282
BssECI CCNNGG 2 cut(s) 121, 568
BssMI GATC 2 cut(s) 109, 855
BssT1I CCWWGG 1 cut(s) 568
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 864
BstBAI YACGTR 1 cut(s) 667
BstC8I GCNNGC 2 cut(s) 284, 408
BstDEI CTNAG 1 cut(s) 428
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 3 cut(s) 301, 427, 868
BstFNI CGCG 3 cut(s) 7, 365, 406
BstHHI GCGC 3 cut(s) 7, 367, 406
BstKTI GATC 2 cut(s) 112, 858
BstMAI GTCTC 1 cut(s) 556
BstMBI GATC 2 cut(s) 109, 855
BstMWI GCNNNNNNNGC 5 cut(s) 44, 158, 260, 334, 847
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 342
BstSFI CTRYAG 1 cut(s) 650
BstUI CGCG 3 cut(s) 7, 365, 406
BstV1I GCAGC 5 cut(s) 275, 442, 575, 636, 749
BstV2I GAAGAC 2 cut(s) 217, 643
BsuI GTATCC 1 cut(s) 174
BsuRI GGCC 3 cut(s) 126, 144, 433
BtgI CCRYGG 1 cut(s) 121
BtsCI GGATG 3 cut(s) 301, 427, 868
BtsI GCAGTG 2 cut(s) 261, 273
BtsIMutI CAGTG 2 cut(s) 261, 273
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 2 cut(s) 284, 408
CfoI GCGC 3 cut(s) 7, 367, 406
Cfr10I RCCGGY 1 cut(s) 282
Cfr13I GGNCC 2 cut(s) 210, 742
CseI GACGC 1 cut(s) 106
Csp6I GTAC 2 cut(s) 202, 290
CviAII CATG 1 cut(s) 320
CviQI GTAC 2 cut(s) 202, 290
DdeI CTNAG 1 cut(s) 428
DpnI GATC 2 cut(s) 111, 857
DpnII GATC 2 cut(s) 109, 855
EciI GGCGGA 2 cut(s) 269, 401
Eco130I CCWWGG 1 cut(s) 568
Eco47I GGWCC 2 cut(s) 210, 742
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 568
FaeI CATG 1 cut(s) 323
FaqI GGGAC 1 cut(s) 494
FatI CATG 1 cut(s) 319
FauI CCCGC 1 cut(s) 183
FauNDI CATATG 1 cut(s) 677
Fnu4HI GCNGC 9 cut(s) 13, 16, 33, 36, 264, 456, 564, 650, 738
FokI GGATG 3 cut(s) 288, 434, 855
Fsp4HI GCNGC 9 cut(s) 13, 16, 33, 36, 264, 456, 564, 650, 738
GlaI GCGC 3 cut(s) 6, 366, 405
GluI GCNGC 9 cut(s) 13, 16, 33, 36, 264, 456, 564, 650, 738
HaeIII GGCC 3 cut(s) 126, 144, 433
HapII CCGG 2 cut(s) 197, 283
HgaI GACGC 1 cut(s) 106
HhaI GCGC 3 cut(s) 7, 367, 406
Hin1II CATG 1 cut(s) 323
Hin6I GCGC 3 cut(s) 5, 365, 404
HinP1I GCGC 3 cut(s) 5, 365, 404
HinfI GANTC 4 cut(s) 26, 62, 626, 757
HpaII CCGG 2 cut(s) 197, 283
HphI GGTGA 2 cut(s) 52, 752
Hpy188I TCNGA 2 cut(s) 474, 701
Hpy188III TCNNGA 1 cut(s) 355
Hpy99I CGWCG 4 cut(s) 70, 100, 152, 158
HpyAV CCTTC 3 cut(s) 214, 585, 816
HpyCH4III ACNGT 1 cut(s) 864
HpyCH4IV ACGT 2 cut(s) 640, 666
HpyCH4V TGCA 7 cut(s) 254, 316, 545, 563, 610, 652, 725
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 158, 260, 334, 847
HpyF3I CTNAG 1 cut(s) 428
HpySE526I ACGT 2 cut(s) 640, 666
Hsp92II CATG 1 cut(s) 323
HspAI GCGC 3 cut(s) 5, 365, 404
KroI GCCGGC 1 cut(s) 282
KroNI GCCGGC 1 cut(s) 284
Kzo9I GATC 2 cut(s) 109, 855
Lsp1109I GCAGC 5 cut(s) 275, 442, 575, 636, 749
LweI GCATC 1 cut(s) 692
MaeII ACGT 2 cut(s) 640, 666
MaeIII GTNAC 1 cut(s) 667
MalI GATC 2 cut(s) 111, 857
MboI GATC 2 cut(s) 109, 855
MboII GAAGA 6 cut(s) 109, 217, 343, 569, 648, 801
MfeI CAATTG 1 cut(s) 170
MluCI AATT 2 cut(s) 170, 238
MlyI GAGTC 3 cut(s) 35, 71, 635
MnlI CCTC 9 cut(s) 103, 137, 146, 155, 288, 423, 649, 704, 852
MroNI GCCGGC 1 cut(s) 282
MroXI GAANNNNTTC 1 cut(s) 224
MseI TTAA 1 cut(s) 830
MspA1I CMGCKG 2 cut(s) 740, 879
MspI CCGG 2 cut(s) 197, 283
MspR9I CCNGG 1 cut(s) 344
MunI CAATTG 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 3 cut(s) 7, 365, 406
MwoI GCNNNNNNNGC 5 cut(s) 44, 158, 260, 334, 847
NaeI GCCGGC 1 cut(s) 284
NdeI CATATG 1 cut(s) 677
NdeII GATC 2 cut(s) 109, 855
NgoMIV GCCGGC 1 cut(s) 282
NlaIII CATG 1 cut(s) 323
NlaIV GGNNCC 1 cut(s) 195
NmeAIII GCCGAG 2 cut(s) 363, 459
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 1 cut(s) 323
PaqCI CACCTGC 1 cut(s) 536
PciI ACATGT 1 cut(s) 319
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 153
PctI GAATGC 1 cut(s) 256
PdiI GCCGGC 1 cut(s) 284
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 1 cut(s) 757
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 9 cut(s) 14, 17, 34, 37, 265, 457, 565, 651, 739
PleI GAGTC 3 cut(s) 34, 70, 634
PpsI GAGTC 3 cut(s) 34, 70, 634
Ppu21I YACGTR 1 cut(s) 667
PscI ACATGT 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 342
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 2 cut(s) 210, 742
PstI CTGCAG 1 cut(s) 654
RsaI GTAC 2 cut(s) 203, 291
RsaNI GTAC 2 cut(s) 202, 290
SaqAI TTAA 1 cut(s) 830
SatI GCNGC 9 cut(s) 13, 16, 33, 36, 264, 456, 564, 650, 738
Sau3AI GATC 2 cut(s) 109, 855
Sau96I GGNCC 2 cut(s) 210, 742
SchI GAGTC 3 cut(s) 35, 71, 635
ScrFI CCNGG 1 cut(s) 344
SfaNI GCATC 1 cut(s) 692
SfcI CTRYAG 1 cut(s) 650
SinI GGWCC 2 cut(s) 210, 742
Sse9I AATT 2 cut(s) 170, 238
StyD4I CCNGG 1 cut(s) 342
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 1 cut(s) 864
TaiI ACGT 2 cut(s) 643, 669
TaqI TCGA 2 cut(s) 68, 95
TasI AATT 2 cut(s) 170, 238
TatI WGTACW 1 cut(s) 289
TauI GCSGC 4 cut(s) 15, 18, 35, 38
TfiI GAWTC 1 cut(s) 757
Tru1I TTAA 1 cut(s) 830
Tru9I TTAA 1 cut(s) 830
TscAI CASTG 2 cut(s) 261, 273
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 5 cut(s) 263, 455, 563, 649, 737
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 2 cut(s) 262, 814
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 2 cut(s) 261, 273
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 2 cut(s) 210, 742
XceI RCATGY 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.