Rh5BG256000

Protein TOM THREE HOMOLOG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006559
Physical Location & Seq
Reverse (-)
31859595 .. 31860904
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG256000.1

Sequence Viewer

Length: 894 bp
ATGGTGCGTAGTGTTGGTGTGGTGCAGTCGGCGGCGGCAGTGATAGCTTACAATCTCAGAGAGTCGTCGAGTTGGTGGCACGACATCAATGCCTCGCCGCTCTGGCAAGACCGCATCTTCCACGGCCTCGCCATTCTCTACGTCCTCGTCGCTTTCGTTGCCCTGGTTCAATTGGTTCGGACACAAGTGCGAGTGCCGGAGTACGGCTGGACCACCCAGAAGGTCTTCCAATTTCTCAATTTTATTGTCAATGCAGTTCGATCAGCAGTGTTTATTTTCCGCCGGCAAGTACAGAAGCTGCATCCAGAGATTCTCCAGCATGTGTTGCTTGATATGCCAAGTCTGGCTTTCTTCACAACTTACGCGCTTCTGGTTCTGTTCTGGGCTGAAATCTACTATAAGGCGCGTGCTGTTTCTACAGATGGACTAAGGCCGAGCTTCTTTACAATCAACGCTGCGGTTTATCTAGTTCAGATATCTATGTGGTTGATATTATGGTGGGACCCTATCGCTGTCGTGGTCTTGTTATCTAAACTGTTCTTCGCAGGGGTCTCCTTATTTGCAGCCCTTGGATTTCTTGTGTATGGTGGAAGACTGTTTTTGATGTTGCAGAGATTTCCTGTGGAATCGAAAGCAAGACGTAAGAAGCTGCATGAGGTTGGCTACGTGACGACCATATGTTTTACGTGTTTCCTTGTGAGAAGCGTAATGATGGGCTTATGTGCATTCGACAAGGCGGCAGACCTTGATGTTTTGAATCACGCAATTCTAAACTTAATGTATTACCTGTTGGTGGAGATATTGCCTTCTTCTTTGGTTCTTTTCATTCTGAGGAAGTTGCCTCCAAAGATTGGGATCACACAATATCAACCTATTCGCGCTCTGAGGGTATGA

Protein Analysis

297

Amino Acids

34.04

Weight (kDa)

9.86

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 22 - 293 1.5e-138 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 851
AccBSI CCGCTC 1 cut(s) 100
AccII CGCG 3 cut(s) 365, 406, 879
AciI CCGC 7 cut(s) 32, 35, 98, 112, 280, 458, 737
AclWI GGATC 1 cut(s) 863
AfaI GTAC 2 cut(s) 203, 291
AfiI CCNNNNNNNGG 3 cut(s) 203, 793, 851
AflIII ACRYGT 1 cut(s) 686
AgsI TTSAA 2 cut(s) 170, 757
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 4 cut(s) 47, 298, 438, 649
AluI AGCT 4 cut(s) 47, 298, 438, 649
Alw26I GTCTC 1 cut(s) 556
AlwI GGATC 1 cut(s) 863
AlwNI CAGNNNCTG 1 cut(s) 298
AoxI GGCC 2 cut(s) 124, 431
ApeKI GCWGC 4 cut(s) 298, 455, 563, 649
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 3 cut(s) 367, 406, 881
AspS9I GGNCC 2 cut(s) 210, 502
AvaII GGWCC 2 cut(s) 210, 502
BbsI GAAGAC 2 cut(s) 217, 598
BbvI GCAGC 4 cut(s) 285, 442, 575, 636
BccI CCATC 2 cut(s) 416, 706
BceAI ACGGC 2 cut(s) 139, 220
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 556
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 1 cut(s) 417
BglI GCCNNNNNGGC 1 cut(s) 103
BisI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
BlsI GCNGC 8 cut(s) 34, 37, 99, 300, 457, 565, 651, 739
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 2 cut(s) 210, 502
BmgT120I GGNCC 2 cut(s) 210, 502
BmiI GGNNCC 2 cut(s) 503, 504
BmrFI CCNGG 1 cut(s) 164
BmsI GCATC 2 cut(s) 123, 310
BpiI GAAGAC 2 cut(s) 217, 598
BpmI CTGGAG 1 cut(s) 299
BsaAI YACGTR 2 cut(s) 667, 687
BsaBI GATNNNNATC 1 cut(s) 854
BsaI GGTCTC 1 cut(s) 556
BsaJI CCNNGG 3 cut(s) 121, 162, 568
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 793, 851
Bse118I RCCGGY 1 cut(s) 282
Bse8I GATNNNNATC 1 cut(s) 854
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 3 cut(s) 121, 162, 568
BseGI GGATG 1 cut(s) 301
BseJI GATNNNNATC 1 cut(s) 854
BseLI CCNNNNNNNGG 3 cut(s) 203, 793, 851
BseMII CTCAG 3 cut(s) 70, 821, 875
BseXI GCAGC 4 cut(s) 285, 442, 575, 636
BsgI GTGCAG 1 cut(s) 44
Bsh1236I CGCG 3 cut(s) 365, 406, 879
BshFI GGCC 2 cut(s) 126, 433
BsiSI CCGG 2 cut(s) 197, 283
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 3 cut(s) 203, 793, 851
BsmAI GTCTC 1 cut(s) 556
BsmFI GGGAC 1 cut(s) 515
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 2 cut(s) 126, 433
Bso31I GGTCTC 1 cut(s) 556
Bsp143I GATC 2 cut(s) 260, 855
BspACI CCGC 7 cut(s) 32, 35, 98, 112, 280, 458, 737
BspANI GGCC 2 cut(s) 126, 433
BspCNI CTCAG 3 cut(s) 69, 822, 876
BspFNI CGCG 3 cut(s) 365, 406, 879
BspLI GGNNCC 2 cut(s) 503, 504
BspPI GGATC 1 cut(s) 863
BspTNI GGTCTC 1 cut(s) 556
BsrBI CCGCTC 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 282
BssAI RCCGGY 1 cut(s) 282
BssECI CCNNGG 3 cut(s) 121, 162, 568
BssMI GATC 2 cut(s) 260, 855
BssT1I CCWWGG 1 cut(s) 568
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 537, 597
BstAPI GCANNNNNTGC 1 cut(s) 325
BstBAI YACGTR 2 cut(s) 667, 687
BstC8I GCNNGC 2 cut(s) 284, 408
BstDEI CTNAG 4 cut(s) 56, 428, 830, 884
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 3 cut(s) 365, 406, 879
BstHHI GCGC 3 cut(s) 367, 406, 881
BstKTI GATC 2 cut(s) 263, 858
BstMAI GTCTC 1 cut(s) 556
BstMBI GATC 2 cut(s) 260, 855
BstMWI GCNNNNNNNGC 5 cut(s) 44, 103, 158, 325, 334
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 162
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 3 cut(s) 365, 406, 879
BstV1I GCAGC 4 cut(s) 285, 442, 575, 636
BstV2I GAAGAC 2 cut(s) 217, 598
BsuRI GGCC 2 cut(s) 126, 433
BtgI CCRYGG 1 cut(s) 121
BtsCI GGATG 1 cut(s) 301
BtsI GCAGTG 2 cut(s) 45, 273
BtsIMutI CAGTG 2 cut(s) 45, 273
Cac8I GCNNGC 2 cut(s) 284, 408
CaiI CAGNNNCTG 1 cut(s) 298
CfoI GCGC 3 cut(s) 367, 406, 881
Cfr10I RCCGGY 1 cut(s) 282
Cfr13I GGNCC 2 cut(s) 210, 502
Csp6I GTAC 2 cut(s) 202, 290
CviAII CATG 2 cut(s) 320, 653
CviQI GTAC 2 cut(s) 202, 290
DdeI CTNAG 4 cut(s) 56, 428, 830, 884
DpnI GATC 2 cut(s) 262, 857
DpnII GATC 2 cut(s) 260, 855
EciI GGCGGA 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 568
Eco31I GGTCTC 1 cut(s) 556
Eco32I GATATC 1 cut(s) 477
Eco47I GGWCC 2 cut(s) 210, 502
EcoO109I RGGNCCY 1 cut(s) 502
EcoRII CCWGG 1 cut(s) 162
EcoRV GATATC 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 568
FaeI CATG 2 cut(s) 323, 656
FalI AAGNNNNNCTT 2 cut(s) 331, 363
FaqI GGGAC 1 cut(s) 515
FatI CATG 2 cut(s) 319, 652
FauNDI CATATG 1 cut(s) 677
Fnu4HI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
FokI GGATG 1 cut(s) 288
Fsp4HI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
FspBI CTAG 1 cut(s) 467
GlaI GCGC 3 cut(s) 366, 405, 880
GluI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
GsuI CTGGAG 1 cut(s) 299
HaeIII GGCC 2 cut(s) 126, 433
HapII CCGG 2 cut(s) 197, 283
HhaI GCGC 3 cut(s) 367, 406, 881
Hin1II CATG 2 cut(s) 323, 656
Hin6I GCGC 3 cut(s) 365, 404, 879
HinP1I GCGC 3 cut(s) 365, 404, 879
HinfI GANTC 4 cut(s) 62, 310, 626, 757
HpaII CCGG 2 cut(s) 197, 283
Hpy188I TCNGA 5 cut(s) 59, 180, 474, 831, 885
Hpy188III TCNNGA 1 cut(s) 305
Hpy99I CGWCG 2 cut(s) 70, 152
HpyAV CCTTC 2 cut(s) 214, 816
HpyCH4III ACNGT 2 cut(s) 537, 597
HpyCH4IV ACGT 4 cut(s) 141, 640, 666, 686
HpyCH4V TGCA 7 cut(s) 25, 254, 301, 563, 610, 652, 725
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 103, 158, 325, 334
HpyF3I CTNAG 4 cut(s) 56, 428, 830, 884
HpySE526I ACGT 4 cut(s) 141, 640, 666, 686
Hsp92II CATG 2 cut(s) 323, 656
HspAI GCGC 3 cut(s) 365, 404, 879
KflI GGGWCCC 1 cut(s) 502
KroI GCCGGC 1 cut(s) 282
KroNI GCCGGC 1 cut(s) 284
Kzo9I GATC 2 cut(s) 260, 855
Lsp1109I GCAGC 4 cut(s) 285, 442, 575, 636
LweI GCATC 2 cut(s) 123, 310
MaeI CTAG 1 cut(s) 467
MaeII ACGT 4 cut(s) 141, 640, 666, 686
MaeIII GTNAC 1 cut(s) 667
MalI GATC 2 cut(s) 262, 857
MbiI CCGCTC 1 cut(s) 100
MboI GATC 2 cut(s) 260, 855
MboII GAAGA 6 cut(s) 109, 217, 343, 532, 603, 801
MfeI CAATTG 1 cut(s) 170
MluCI AATT 4 cut(s) 170, 230, 238, 765
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 7 cut(s) 103, 137, 155, 649, 825, 852, 879
MroNI GCCGGC 1 cut(s) 282
MroXI GAANNNNTTC 1 cut(s) 224
MseI TTAA 1 cut(s) 776
MspI CCGG 2 cut(s) 197, 283
MspR9I CCNGG 1 cut(s) 164
MunI CAATTG 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 725
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 3 cut(s) 365, 406, 879
MwoI GCNNNNNNNGC 5 cut(s) 44, 103, 158, 325, 334
NaeI GCCGGC 1 cut(s) 284
NdeI CATATG 1 cut(s) 677
NdeII GATC 2 cut(s) 260, 855
NgoMIV GCCGGC 1 cut(s) 282
NlaIII CATG 2 cut(s) 323, 656
NlaIV GGNNCC 2 cut(s) 503, 504
NmeAIII GCCGAG 1 cut(s) 459
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 1 cut(s) 323
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 725
PdiI GCCGGC 1 cut(s) 284
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 3 cut(s) 310, 626, 757
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 8 cut(s) 34, 37, 99, 300, 457, 565, 651, 739
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Ppu21I YACGTR 2 cut(s) 667, 687
PpuMI RGGWCCY 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 502
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 503, 504
PspPI GGNCC 2 cut(s) 210, 502
PspPPI RGGWCCY 1 cut(s) 502
PstNI CAGNNNCTG 1 cut(s) 298
RsaI GTAC 2 cut(s) 203, 291
RsaNI GTAC 2 cut(s) 202, 290
SaqAI TTAA 1 cut(s) 776
SatI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
Sau3AI GATC 2 cut(s) 260, 855
Sau96I GGNCC 2 cut(s) 210, 502
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 164
SfaNI GCATC 2 cut(s) 123, 310
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 2 cut(s) 210, 502
Sse9I AATT 4 cut(s) 170, 230, 238, 765
SsiI CCGC 7 cut(s) 32, 35, 98, 112, 280, 458, 737
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 2 cut(s) 537, 597
TaiI ACGT 4 cut(s) 144, 643, 669, 689
TaqI TCGA 4 cut(s) 68, 259, 629, 729
TasI AATT 4 cut(s) 170, 230, 238, 765
TatI WGTACW 1 cut(s) 289
TauI GCSGC 4 cut(s) 35, 38, 100, 740
TfiI GAWTC 3 cut(s) 310, 626, 757
Tru1I TTAA 1 cut(s) 776
Tru9I TTAA 1 cut(s) 776
TscAI CASTG 2 cut(s) 45, 273
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 4 cut(s) 298, 455, 563, 649
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 1 cut(s) 814
TspRI CASTG 2 cut(s) 45, 273
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 2 cut(s) 210, 502
XceI RCATGY 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.