MD16G1008000.v1.1

Protein TOM THREE HOMOLOG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006559
Physical Location & Seq
Reverse (-)
593109 .. 598456
5348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1008000.v1.1.491

Sequence Viewer

Length: 888 bp
ATGGCGCGTAGCGGCGGCGGCGGAGTCGGGGTGGTGGAGTCGGCGGTGATAGCGTACAACCTGAGAGACGCATCGAGTTGGTGGCACGACATCAATGCCTCCCCTATTTGGCAAAACCGCATATTTCATGCCCTCGCTATTCTCTACGGTCTCGTCGCCGTCGTTGCCCTGGTTCAATTGATTCGGATACAATTGAGAGTGCCGGAGTACGGTTGGACCACGCAGAAAGTATTCCACTTTCTTAATTTTGTTGTCAATGGAGTTCGATCAGCAGTGTTTGTGTTCCGCTGGCAAGTGCAGAAGCTGCATCCAGAGATTGTCAAACATATCTTGCTTGATATGCCGAGTCTTGCCTTCTTCACTACCTATGCGCTCTTGGTTCTTTTCTGGGCTGAAATTTACTATCAGGCACGCACTGTTTCTACAGATGGACTGAGGCCAAGCTTCTTGACTGTCAATGCTGTGGTTTATGTGATTCAGATTGCTATGTGGTTGATATTATGGTGGAAGCCTATCCCAGTTCTGCTCACCTTATCTAAGATGTTCTTTGCAGGTGTGTCCTTATTCGCAGCCCTTGGGTTTCTTCTATATGGTGGAAGACTCTTCTTGATGTTGCAGAGATTTCCTGTAGAATCAAAAGGACGACGTAAGAAGCTGCAGGAGGTTGGCTATGTGACAACCATATGTTTTACATGTTTCCTTGTCAGATGCATAATGATGTGCTTCAATGCATTCGATGAAGCTGCTGACCTTGATGTTTTGAATCACCCGGTTCTAAACTTTATATATTACCTATTGGTGGAGATATTGCCTTCTTCTTTGGTCCTTTTCATTTTGAGGAAGTTGCCTCCAAAGCGTGGGATCACACAATATCACCCGATTCGCTGA

Protein Analysis

296

Amino Acids

33.85

Weight (kDa)

9.72

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 24 - 295 8.5e-148 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 542
Acc36I ACCTGC 1 cut(s) 542
AccB7I CCANNNNNTGG 1 cut(s) 857
AccII CGCG 1 cut(s) 7
AciI CCGC 7 cut(s) 12, 15, 18, 21, 44, 118, 286
AclWI GGATC 1 cut(s) 869
AcsI RAATTY 1 cut(s) 396
AfaI GTAC 2 cut(s) 56, 209
AfiI CCNNNNNNNGG 4 cut(s) 108, 209, 799, 857
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 3 cut(s) 176, 727, 763
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 4 cut(s) 304, 444, 655, 743
AluI AGCT 4 cut(s) 304, 444, 655, 743
Alw26I GTCTC 2 cut(s) 60, 155
AlwI GGATC 1 cut(s) 869
AlwNI CAGNNNCTG 1 cut(s) 304
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 4 cut(s) 304, 569, 655, 743
ApoI RAATTY 1 cut(s) 396
Asp700I GAANNNNTTC 1 cut(s) 230
AspLEI GCGC 2 cut(s) 7, 373
AspS9I GGNCC 2 cut(s) 216, 823
AsuC2I CCSGG 1 cut(s) 770
AsuHPI GGTGA 4 cut(s) 58, 520, 758, 866
AvaII GGWCC 2 cut(s) 216, 823
BbsI GAAGAC 1 cut(s) 604
BbvI GCAGC 4 cut(s) 291, 581, 642, 730
BccI CCATC 1 cut(s) 422
BceAI ACGGC 1 cut(s) 143
BcgI CGANNNNNNTGC 4 cut(s) 77, 111, 725, 759
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 180
BcnI CCSGG 1 cut(s) 770
BcoDI GTCTC 2 cut(s) 60, 155
BfmI CTRYAG 3 cut(s) 423, 627, 656
BfuAI ACCTGC 1 cut(s) 542
BfuI GTATCC 1 cut(s) 180
BisI GCNGC 7 cut(s) 13, 16, 19, 305, 570, 656, 744
BlsI GCNGC 7 cut(s) 14, 17, 20, 306, 571, 657, 745
Bme1390I CCNGG 2 cut(s) 170, 770
Bme18I GGWCC 2 cut(s) 216, 823
BmgT120I GGNCC 2 cut(s) 216, 823
BmrFI CCNGG 2 cut(s) 170, 770
BmrI ACTGGG 1 cut(s) 512
BmsI GCATC 3 cut(s) 80, 316, 698
BmuI ACTGGG 1 cut(s) 512
BpiI GAAGAC 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 770
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 168, 574
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 209, 799, 857
Bse1I ACTGG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 2 cut(s) 168, 574
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 4 cut(s) 108, 209, 799, 857
BseMII CTCAG 2 cut(s) 53, 425
BseNI ACTGG 1 cut(s) 518
BseXI GCAGC 4 cut(s) 291, 581, 642, 730
BsgI GTGCAG 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 1 cut(s) 439
BsiSI CCGG 2 cut(s) 203, 770
BslI CCNNNNNNNGG 4 cut(s) 108, 209, 799, 857
BsmAI GTCTC 2 cut(s) 60, 155
BsmBI CGTCTC 1 cut(s) 60
BsmI GAATGC 1 cut(s) 731
BsnI GGCC 1 cut(s) 439
Bso31I GGTCTC 1 cut(s) 155
Bsp143I GATC 2 cut(s) 266, 861
BspACI CCGC 7 cut(s) 12, 15, 18, 21, 44, 118, 286
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 2 cut(s) 54, 426
BspFNI CGCG 1 cut(s) 7
BspMAI CTGCAG 1 cut(s) 660
BspMI ACCTGC 1 cut(s) 542
BspPI GGATC 1 cut(s) 869
BspTNI GGTCTC 1 cut(s) 155
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 168, 574
BssMI GATC 2 cut(s) 266, 861
BssT1I CCWWGG 1 cut(s) 574
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 4 cut(s) 149, 212, 418, 454
Bst6I CTCTTC 1 cut(s) 608
BstAPI GCANNNNNTGC 1 cut(s) 304
BstC8I GCNNGC 2 cut(s) 290, 412
BstDEI CTNAG 3 cut(s) 62, 434, 537
BstF5I GGATG 1 cut(s) 307
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 2 cut(s) 7, 373
BstKTI GATC 2 cut(s) 269, 864
BstMAI GTCTC 2 cut(s) 60, 155
BstMBI GATC 2 cut(s) 266, 861
BstMWI GCNNNNNNNGC 6 cut(s) 18, 50, 164, 304, 340, 853
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 696
BstSCI CCNGG 2 cut(s) 168, 768
BstSFI CTRYAG 3 cut(s) 423, 627, 656
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 4 cut(s) 291, 581, 642, 730
BstV2I GAAGAC 1 cut(s) 604
BsuI GTATCC 1 cut(s) 180
BsuRI GGCC 1 cut(s) 439
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 279, 414
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 2 cut(s) 290, 412
CaiI CAGNNNCTG 1 cut(s) 304
CfoI GCGC 2 cut(s) 7, 373
Cfr13I GGNCC 2 cut(s) 216, 823
CseI GACGC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 55, 208
CviAII CATG 2 cut(s) 128, 693
CviJI RGCY 9 cut(s) 304, 392, 439, 444, 511, 572, 655, 669, 743
CviKI_1 RGCY 9 cut(s) 304, 392, 439, 444, 511, 572, 655, 669, 743
CviQI GTAC 2 cut(s) 55, 208
DdeI CTNAG 3 cut(s) 62, 434, 537
DpnI GATC 2 cut(s) 268, 863
DpnII GATC 2 cut(s) 266, 861
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
EciI GGCGGA 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 574
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 216, 823
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 574
EcoT22I ATGCAT 2 cut(s) 713, 733
ErhI CCWWGG 1 cut(s) 574
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 2 cut(s) 131, 696
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FatI CATG 2 cut(s) 127, 692
FauNDI CATATG 1 cut(s) 683
Fnu4HI GCNGC 7 cut(s) 13, 16, 19, 305, 570, 656, 744
FokI GGATG 1 cut(s) 294
Fsp4HI GCNGC 7 cut(s) 13, 16, 19, 305, 570, 656, 744
GlaI GCGC 2 cut(s) 6, 372
GluI GCNGC 7 cut(s) 13, 16, 19, 305, 570, 656, 744
HaeIII GGCC 1 cut(s) 439
HapII CCGG 2 cut(s) 203, 770
HgaI GACGC 1 cut(s) 77
HhaI GCGC 2 cut(s) 7, 373
Hin1II CATG 2 cut(s) 131, 696
Hin6I GCGC 2 cut(s) 5, 371
HinP1I GCGC 2 cut(s) 5, 371
HindIII AAGCTT 1 cut(s) 442
HinfI GANTC 9 cut(s) 24, 38, 181, 346, 475, 600, 632, 763, 880
HpaII CCGG 2 cut(s) 203, 770
HphI GGTGA 4 cut(s) 58, 520, 758, 866
Hpy188I TCNGA 3 cut(s) 186, 480, 707
Hpy188III TCNNGA 3 cut(s) 311, 448, 607
Hpy99I CGWCG 3 cut(s) 158, 164, 648
HpyAV CCTTC 2 cut(s) 364, 822
HpyCH4III ACNGT 4 cut(s) 149, 212, 418, 454
HpyCH4IV ACGT 1 cut(s) 646
HpyCH4V TGCA 7 cut(s) 298, 307, 551, 616, 658, 711, 731
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 50, 164, 304, 340, 853
HpyF3I CTNAG 3 cut(s) 62, 434, 537
HpySE526I ACGT 1 cut(s) 646
Hsp92II CATG 2 cut(s) 131, 696
HspAI GCGC 2 cut(s) 5, 371
Kzo9I GATC 2 cut(s) 266, 861
Lsp1109I GCAGC 4 cut(s) 291, 581, 642, 730
LweI GCATC 3 cut(s) 80, 316, 698
MaeII ACGT 1 cut(s) 646
MaeIII GTNAC 1 cut(s) 673
MalI GATC 2 cut(s) 268, 863
MboI GATC 2 cut(s) 266, 861
MboII GAAGA 5 cut(s) 349, 575, 595, 609, 807
MfeI CAATTG 2 cut(s) 176, 191
MluCI AATT 4 cut(s) 176, 191, 244, 396
MlyI GAGTC 4 cut(s) 33, 47, 355, 594
MmeI TCCRAC 1 cut(s) 194
MnlI CCTC 6 cut(s) 109, 143, 429, 655, 831, 858
Mph1103I ATGCAT 2 cut(s) 713, 733
MroXI GAANNNNTTC 1 cut(s) 230
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 2 cut(s) 203, 770
MspR9I CCNGG 2 cut(s) 170, 770
MunI CAATTG 2 cut(s) 176, 191
Mva1269I GAATGC 1 cut(s) 731
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 6 cut(s) 18, 50, 164, 304, 340, 853
NciI CCSGG 1 cut(s) 770
NdeI CATATG 1 cut(s) 683
NdeII GATC 2 cut(s) 266, 861
NlaIII CATG 2 cut(s) 131, 696
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 673
NsiI ATGCAT 2 cut(s) 713, 733
NspI RCATGY 1 cut(s) 696
PaqCI CACCTGC 1 cut(s) 542
PciI ACATGT 1 cut(s) 692
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 731
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 5 cut(s) 181, 475, 632, 763, 880
PflMI CCANNNNNTGG 1 cut(s) 857
PkrI GCNGC 7 cut(s) 14, 17, 20, 306, 571, 657, 745
PleI GAGTC 4 cut(s) 32, 46, 354, 594
PpsI GAGTC 4 cut(s) 32, 46, 354, 594
PscI ACATGT 1 cut(s) 692
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspPI GGNCC 2 cut(s) 216, 823
PstI CTGCAG 1 cut(s) 660
PstNI CAGNNNCTG 1 cut(s) 304
RsaI GTAC 2 cut(s) 56, 209
RsaNI GTAC 2 cut(s) 55, 208
RseI CAYNNNNRTG 1 cut(s) 716
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 7 cut(s) 13, 16, 19, 305, 570, 656, 744
Sau3AI GATC 2 cut(s) 266, 861
Sau96I GGNCC 2 cut(s) 216, 823
SchI GAGTC 4 cut(s) 33, 47, 355, 594
ScrFI CCNGG 2 cut(s) 170, 770
SfaNI GCATC 3 cut(s) 80, 316, 698
SfcI CTRYAG 3 cut(s) 423, 627, 656
SinI GGWCC 2 cut(s) 216, 823
SmiMI CAYNNNNRTG 1 cut(s) 716
Sse9I AATT 4 cut(s) 176, 191, 244, 396
SsiI CCGC 7 cut(s) 12, 15, 18, 21, 44, 118, 286
StyD4I CCNGG 2 cut(s) 168, 768
StyI CCWWGG 1 cut(s) 574
TaaI ACNGT 4 cut(s) 149, 212, 418, 454
TaiI ACGT 1 cut(s) 649
TaqI TCGA 3 cut(s) 74, 265, 735
TasI AATT 4 cut(s) 176, 191, 244, 396
TauI GCSGC 3 cut(s) 15, 18, 21
TfiI GAWTC 5 cut(s) 181, 475, 632, 763, 880
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 2 cut(s) 279, 421
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 4 cut(s) 304, 569, 655, 743
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 3 cut(s) 116, 753, 820
TspRI CASTG 2 cut(s) 279, 421
Van91I CCANNNNNTGG 1 cut(s) 857
VpaK11BI GGWCC 2 cut(s) 216, 823
XapI RAATTY 1 cut(s) 396
XceI RCATGY 1 cut(s) 696
XmnI GAANNNNTTC 1 cut(s) 230
Zsp2I ATGCAT 2 cut(s) 713, 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.