Rh5CG289100

Protein TOM THREE HOMOLOG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0006559
Physical Location & Seq
Reverse (-)
31658863 .. 31660031
1169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG289100.1

Sequence Viewer

Length: 894 bp
ATGGTGCGTAGCGTTGGTGTGGTGCAGTCGGCGGCGGCAGTGATAGCTTACGATCTCAGAGAGTCGTCGAGTTGGTGGCACGACATCAATGCCTCGCCGCTCTGGCAAGGCCGAATCTTCCACGGCCTTGCCATTCTCTACGTCCTCGTCGCTTTCGTTGCCCTGGTTCAATTGGTTCGGACACTAGTGCGAGTGCCGGAGTACGGCTGGACCACCCAGAAGGTCTTCCAATTTCTCAATTTTATTGTCAATGCAGTTCGATCAGCAGTGTTTATTTTCCGCCGGCAAGTGCAGAAGCTGCATCCAGAGATTCTCCAACATGTGTTGCTTGATATGCCAAGTCTGGCTTTCTTCACAACTTACGCTCTTCTGGTTCTGTTCTGGGCTGAAATCTACTATAAGGCGCGTGCTGTTTCTACAGATGGACTAAGGCCGAGCTTCTTTACAATCAACGCTGCGGTTTATCTAGTTCAGATATCTATGTGGTTGATATTATGGTGGAACCCTATCCCAGTCGTGGTCTTGTTATCTAAACTGTTCTTCGCAGGGGTCTCCTTATTTGCAGCCCTTGGATTTCTTGTGTATGGTGGAAGACTGTTTCTGATGTTGCAGAGATTTCCTGTGGAATCGAAAGCAAGACGTAAGAAGCTGCATGAGGTTGGCTACGTGACAACCATATGTTTTACTTGTTTCCTTGTGAGATGCGTAATGATGGGCTTATGTGCATTCGACAAGGCGGCAGACCTTGATGTTTTGAATCACCCAGTTCTAAACTTGATCTATTACCTGTTGGTGGAGATATTGCCTTCTTCTTTGGTTCTTTTCATTCTGAGGAAGTTGCCTCCAAAGATTGGGGTCACACAATATCAGCCTATTCGCGCTCTGAAGGTATGA

Protein Analysis

297

Amino Acids

33.96

Weight (kDa)

9.84

Isoelectric Point (pI)

41.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 22 - 293 1e-138 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 851
AccBSI CCGCTC 1 cut(s) 100
AccII CGCG 2 cut(s) 406, 879
AciI CCGC 6 cut(s) 32, 35, 98, 280, 458, 737
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 4 cut(s) 203, 517, 793, 851
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 2 cut(s) 170, 757
AhlI ACTAGT 1 cut(s) 184
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 4 cut(s) 47, 298, 438, 649
AluI AGCT 4 cut(s) 47, 298, 438, 649
Alw26I GTCTC 1 cut(s) 556
AlwNI CAGNNNCTG 1 cut(s) 298
AoxI GGCC 3 cut(s) 109, 124, 431
ApeKI GCWGC 4 cut(s) 298, 455, 563, 649
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 2 cut(s) 406, 881
AspS9I GGNCC 1 cut(s) 210
AsuHPI GGTGA 1 cut(s) 752
AvaII GGWCC 1 cut(s) 210
BbsI GAAGAC 2 cut(s) 217, 598
BbvI GCAGC 4 cut(s) 285, 442, 575, 636
BccI CCATC 2 cut(s) 416, 706
BceAI ACGGC 2 cut(s) 139, 220
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 556
BcuI ACTAGT 1 cut(s) 184
BfaI CTAG 2 cut(s) 185, 467
BfmI CTRYAG 1 cut(s) 417
BglI GCCNNNNNGGC 1 cut(s) 103
BisI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
BlsI GCNGC 8 cut(s) 34, 37, 99, 300, 457, 565, 651, 739
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 1 cut(s) 210
BmgT120I GGNCC 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 503
BmrFI CCNGG 1 cut(s) 164
BmrI ACTGGG 2 cut(s) 506, 758
BmsI GCATC 2 cut(s) 310, 692
BmuI ACTGGG 2 cut(s) 506, 758
BpiI GAAGAC 2 cut(s) 217, 598
BsaAI YACGTR 1 cut(s) 667
BsaI GGTCTC 1 cut(s) 556
BsaJI CCNNGG 3 cut(s) 121, 162, 568
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 517, 793, 851
Bse118I RCCGGY 1 cut(s) 282
Bse1I ACTGG 2 cut(s) 512, 764
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 3 cut(s) 121, 162, 568
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 4 cut(s) 203, 517, 793, 851
BseMII CTCAG 2 cut(s) 70, 821
BseNI ACTGG 2 cut(s) 512, 764
BseXI GCAGC 4 cut(s) 285, 442, 575, 636
BsgI GTGCAG 2 cut(s) 44, 311
Bsh1236I CGCG 2 cut(s) 406, 879
BshFI GGCC 3 cut(s) 111, 126, 433
BsiSI CCGG 2 cut(s) 197, 283
BslI CCNNNNNNNGG 4 cut(s) 203, 517, 793, 851
BsmAI GTCTC 1 cut(s) 556
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 3 cut(s) 111, 126, 433
Bso31I GGTCTC 1 cut(s) 556
Bsp143I GATC 3 cut(s) 52, 260, 777
BspACI CCGC 6 cut(s) 32, 35, 98, 280, 458, 737
BspANI GGCC 3 cut(s) 111, 126, 433
BspCNI CTCAG 2 cut(s) 69, 822
BspFNI CGCG 2 cut(s) 406, 879
BspLI GGNNCC 1 cut(s) 503
BspQI GCTCTTC 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 556
BsrBI CCGCTC 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 282
BsrI ACTGG 2 cut(s) 512, 764
BssAI RCCGGY 1 cut(s) 282
BssECI CCNNGG 3 cut(s) 121, 162, 568
BssMI GATC 3 cut(s) 52, 260, 777
BssT1I CCWWGG 1 cut(s) 568
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 537, 597
Bst6I CTCTTC 1 cut(s) 372
BstAPI GCANNNNNTGC 1 cut(s) 298
BstBAI YACGTR 1 cut(s) 667
BstC8I GCNNGC 2 cut(s) 284, 408
BstDEI CTNAG 3 cut(s) 56, 428, 830
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 2 cut(s) 406, 879
BstHHI GCGC 2 cut(s) 406, 881
BstKTI GATC 3 cut(s) 55, 263, 780
BstMAI GTCTC 1 cut(s) 556
BstMBI GATC 3 cut(s) 52, 260, 777
BstMWI GCNNNNNNNGC 5 cut(s) 44, 103, 158, 298, 334
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 162
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 2 cut(s) 406, 879
BstV1I GCAGC 4 cut(s) 285, 442, 575, 636
BstV2I GAAGAC 2 cut(s) 217, 598
BsuRI GGCC 3 cut(s) 111, 126, 433
BtgI CCRYGG 1 cut(s) 121
BtsCI GGATG 1 cut(s) 301
BtsI GCAGTG 2 cut(s) 45, 273
BtsIMutI CAGTG 2 cut(s) 45, 273
Cac8I GCNNGC 2 cut(s) 284, 408
CaiI CAGNNNCTG 1 cut(s) 298
CfoI GCGC 2 cut(s) 406, 881
Cfr10I RCCGGY 1 cut(s) 282
Cfr13I GGNCC 1 cut(s) 210
Csp6I GTAC 1 cut(s) 202
CviAII CATG 2 cut(s) 320, 653
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 3 cut(s) 56, 428, 830
DpnI GATC 3 cut(s) 54, 262, 779
DpnII GATC 3 cut(s) 52, 260, 777
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
EciI GGCGGA 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 568
Eco31I GGTCTC 1 cut(s) 556
Eco32I GATATC 1 cut(s) 477
Eco47I GGWCC 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 162
EcoRV GATATC 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 568
FaeI CATG 2 cut(s) 323, 656
FalI AAGNNNNNCTT 2 cut(s) 331, 363
FatI CATG 2 cut(s) 319, 652
FauNDI CATATG 1 cut(s) 677
Fnu4HI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
FokI GGATG 1 cut(s) 288
Fsp4HI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
FspBI CTAG 2 cut(s) 185, 467
GlaI GCGC 2 cut(s) 405, 880
GluI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
HaeIII GGCC 3 cut(s) 111, 126, 433
HapII CCGG 2 cut(s) 197, 283
HhaI GCGC 2 cut(s) 406, 881
Hin1II CATG 2 cut(s) 323, 656
Hin6I GCGC 2 cut(s) 404, 879
HinP1I GCGC 2 cut(s) 404, 879
HinfI GANTC 5 cut(s) 62, 114, 310, 626, 757
HpaII CCGG 2 cut(s) 197, 283
HphI GGTGA 1 cut(s) 752
Hpy188I TCNGA 6 cut(s) 59, 180, 474, 603, 831, 885
Hpy188III TCNNGA 1 cut(s) 305
Hpy99I CGWCG 2 cut(s) 70, 152
HpyAV CCTTC 3 cut(s) 214, 816, 880
HpyCH4III ACNGT 2 cut(s) 537, 597
HpyCH4IV ACGT 3 cut(s) 141, 640, 666
HpyCH4V TGCA 8 cut(s) 25, 254, 292, 301, 563, 610, 652, 725
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 103, 158, 298, 334
HpyF3I CTNAG 3 cut(s) 56, 428, 830
HpySE526I ACGT 3 cut(s) 141, 640, 666
Hsp92II CATG 2 cut(s) 323, 656
HspAI GCGC 2 cut(s) 404, 879
KroI GCCGGC 1 cut(s) 282
KroNI GCCGGC 1 cut(s) 284
Kzo9I GATC 3 cut(s) 52, 260, 777
LguI GCTCTTC 1 cut(s) 372
Lsp1109I GCAGC 4 cut(s) 285, 442, 575, 636
LweI GCATC 2 cut(s) 310, 692
MaeI CTAG 2 cut(s) 185, 467
MaeII ACGT 3 cut(s) 141, 640, 666
MaeIII GTNAC 2 cut(s) 667, 856
MalI GATC 3 cut(s) 54, 262, 779
MbiI CCGCTC 1 cut(s) 100
MboI GATC 3 cut(s) 52, 260, 777
MboII GAAGA 7 cut(s) 109, 217, 343, 359, 532, 603, 801
MfeI CAATTG 1 cut(s) 170
MluCI AATT 3 cut(s) 170, 230, 238
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 5 cut(s) 103, 155, 649, 825, 852
MroNI GCCGGC 1 cut(s) 282
MroXI GAANNNNTTC 1 cut(s) 224
MspI CCGG 2 cut(s) 197, 283
MspR9I CCNGG 1 cut(s) 164
MunI CAATTG 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 725
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 2 cut(s) 406, 879
MwoI GCNNNNNNNGC 5 cut(s) 44, 103, 158, 298, 334
NaeI GCCGGC 1 cut(s) 284
NdeI CATATG 1 cut(s) 677
NdeII GATC 3 cut(s) 52, 260, 777
NgoMIV GCCGGC 1 cut(s) 282
NlaIII CATG 2 cut(s) 323, 656
NlaIV GGNNCC 1 cut(s) 503
NmeAIII GCCGAG 1 cut(s) 459
NmuCI GTSAC 2 cut(s) 667, 856
NspI RCATGY 1 cut(s) 323
PciI ACATGT 1 cut(s) 319
PciSI GCTCTTC 1 cut(s) 372
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 725
PdiI GCCGGC 1 cut(s) 284
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 4 cut(s) 114, 310, 626, 757
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 8 cut(s) 34, 37, 99, 300, 457, 565, 651, 739
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Ppu21I YACGTR 1 cut(s) 667
PscI ACATGT 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 503
PspPI GGNCC 1 cut(s) 210
PstNI CAGNNNCTG 1 cut(s) 298
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SapI GCTCTTC 1 cut(s) 372
SatI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
Sau3AI GATC 3 cut(s) 52, 260, 777
Sau96I GGNCC 1 cut(s) 210
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 164
SfaNI GCATC 2 cut(s) 310, 692
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 1 cut(s) 210
SpeI ACTAGT 1 cut(s) 184
Sse9I AATT 3 cut(s) 170, 230, 238
SsiI CCGC 6 cut(s) 32, 35, 98, 280, 458, 737
SspMI CTAG 2 cut(s) 185, 467
StyD4I CCNGG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 2 cut(s) 537, 597
TaiI ACGT 3 cut(s) 144, 643, 669
TaqI TCGA 4 cut(s) 68, 259, 629, 729
TasI AATT 3 cut(s) 170, 230, 238
TauI GCSGC 4 cut(s) 35, 38, 100, 740
TfiI GAWTC 4 cut(s) 114, 310, 626, 757
TscAI CASTG 2 cut(s) 45, 273
TseFI GTSAC 2 cut(s) 667, 856
TseI GCWGC 4 cut(s) 298, 455, 563, 649
Tsp45I GTSAC 2 cut(s) 667, 856
TspDTI ATGAA 1 cut(s) 814
TspRI CASTG 2 cut(s) 45, 273
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 1 cut(s) 210
XceI RCATGY 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 2 cut(s) 185, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.