Rroxscaffold_5G00386500

Protein TOM THREE HOMOLOG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006559
Physical Location & Seq
Forward (+)
65393719 .. 65399636
5918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386500.1

Sequence Viewer

Length: 894 bp
ATGGCGCGCGGCGGCGGCGGCGGCGGAAGCGTGGTGGAGTCGGCGGCGGCGGTGATAGCTTACAATCTGAAAGACTCGTCGAGTTGGTGGCACGACATCAATGCCTCGCAGCTCTGGCAAGACCGAATCTTCCACGGCCTCGCCATTCTCTACGGCCTCGTCGCCGTCGTTGCCCTGGTTCAATTGATTCGGATACAAGTGCGGGTGCCGGAGTACGGCTGGACCACTCAGAAGGTCTTCCACTTTCTCAATTTTGTTGTCAACGCAGTTCGAGCAGCAGTGTTCATTTTCCGCCGGCAAGTACAGAAGCTGCATCCAGAGATTCTCCAACATGTATTGCTTGATATGCCGAGTCTGGCTTTCTTCACTACTTACGCGCTCCTGGTTCTGTTCTGGGCAGAAATTTACTATCAGGCGCGTGCTGTTTCTACAGATGGACTGAGGCCGAGCTTCTTTACAATCAATGCTGCCGTTTATGTAATTCAGATATCTATGTGGTTGATATTATGGTGGAAGCCTATCCCAGTCATGGTCATCTTATCTAAAATGTTCTTTGCAGGTGTCTCCTTATTTGCAGCCCTTGGATTTCTACTGTATGGTGGAAGACTCTTTTTGATGTTGCAGAGATTTCCTGTGGAGTCAAAAGGAAGACGTAAGAAGCTGCAGGAGGTTGGCTACGTGACAACCATATGTTTTACTTGTTTCCTTGTCAGATGCGTAATGATGTGCTTATGTGCATTTGACAAGGCGGCTGACCTTGATGTTTTGAATCACCCAGTTCTAAACTTAATATATTACCTGTTGGTGGAGATATTGCCTTCTTCTTTGGTTCTTTTCATTTTGAGGAAGTTGCCTCCAAAGCGTGGGATCACACAATATCATCCTATTCGCTGA

Protein Analysis

297

Amino Acids

33.85

Weight (kDa)

9.71

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 26 - 297 6.9e-149 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 548
Acc36I ACCTGC 1 cut(s) 548
AccB1I GGYRCC 1 cut(s) 205
AccB7I CCANNNNNTGG 1 cut(s) 863
AccII CGCG 4 cut(s) 7, 9, 377, 418
AclWI GGATC 1 cut(s) 875
AcsI RAATTY 1 cut(s) 402
AfaI GTAC 2 cut(s) 215, 303
AfiI CCNNNNNNNGG 4 cut(s) 215, 529, 805, 863
AflIII ACRYGT 1 cut(s) 331
AgsI TTSAA 2 cut(s) 182, 769
AjnI CCWGG 2 cut(s) 174, 381
AluBI AGCT 5 cut(s) 59, 112, 310, 450, 661
AluI AGCT 5 cut(s) 59, 112, 310, 450, 661
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 1 cut(s) 875
AlwNI CAGNNNCTG 1 cut(s) 310
AoxI GGCC 3 cut(s) 136, 154, 443
ApeKI GCWGC 6 cut(s) 109, 275, 310, 467, 575, 661
ApoI RAATTY 1 cut(s) 402
Asp700I GAANNNNTTC 1 cut(s) 236
AspLEI GCGC 4 cut(s) 7, 9, 379, 418
AspS9I GGNCC 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 64, 764
AvaII GGWCC 1 cut(s) 222
BanI GGYRCC 1 cut(s) 205
BbsI GAAGAC 3 cut(s) 229, 610, 655
BbvI GCAGC 6 cut(s) 121, 287, 297, 454, 587, 648
BccI CCATC 1 cut(s) 428
BceAI ACGGC 5 cut(s) 149, 151, 169, 232, 455
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 2 cut(s) 176, 383
BciVI GTATCC 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 568
BfmI CTRYAG 2 cut(s) 429, 662
BfuAI ACCTGC 1 cut(s) 548
BfuI GTATCC 1 cut(s) 186
Bme1390I CCNGG 2 cut(s) 176, 383
Bme18I GGWCC 1 cut(s) 222
BmgT120I GGNCC 1 cut(s) 222
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 2 cut(s) 176, 383
BmrI ACTGGG 2 cut(s) 518, 770
BmsI GCATC 2 cut(s) 322, 704
BmuI ACTGGG 2 cut(s) 518, 770
BpiI GAAGAC 3 cut(s) 229, 610, 655
BsaAI YACGTR 1 cut(s) 679
BsaJI CCNNGG 3 cut(s) 133, 174, 580
Bsc4I CCNNNNNNNGG 4 cut(s) 215, 529, 805, 863
Bse118I RCCGGY 1 cut(s) 294
Bse1I ACTGG 2 cut(s) 524, 776
BseBI CCWGG 2 cut(s) 176, 383
BseDI CCNNGG 3 cut(s) 133, 174, 580
BseGI GGATG 2 cut(s) 313, 880
BseLI CCNNNNNNNGG 4 cut(s) 215, 529, 805, 863
BseMII CTCAG 2 cut(s) 242, 431
BseNI ACTGG 2 cut(s) 524, 776
BsePI GCGCGC 1 cut(s) 5
BseXI GCAGC 6 cut(s) 121, 287, 297, 454, 587, 648
Bsh1236I CGCG 4 cut(s) 7, 9, 377, 418
BshFI GGCC 3 cut(s) 138, 156, 445
BshNI GGYRCC 1 cut(s) 205
BsiSI CCGG 2 cut(s) 209, 295
BslI CCNNNNNNNGG 4 cut(s) 215, 529, 805, 863
BsmAI GTCTC 1 cut(s) 568
BsnI GGCC 3 cut(s) 138, 156, 445
Bsp143I GATC 1 cut(s) 867
BspANI GGCC 3 cut(s) 138, 156, 445
BspCNI CTCAG 2 cut(s) 241, 432
BspFNI CGCG 4 cut(s) 7, 9, 377, 418
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 1 cut(s) 666
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 1 cut(s) 875
BspT107I GGYRCC 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 294
BsrI ACTGG 2 cut(s) 524, 776
BssAI RCCGGY 1 cut(s) 294
BssECI CCNNGG 3 cut(s) 133, 174, 580
BssHII GCGCGC 1 cut(s) 5
BssMI GATC 1 cut(s) 867
BssT1I CCWWGG 1 cut(s) 580
Bst2UI CCWGG 2 cut(s) 176, 383
Bst4CI ACNGT 1 cut(s) 594
BstBAI YACGTR 1 cut(s) 679
BstC8I GCNNGC 3 cut(s) 7, 296, 420
BstDEI CTNAG 2 cut(s) 228, 440
BstDSI CCRYGG 1 cut(s) 133
BstF5I GGATG 2 cut(s) 313, 880
BstFNI CGCG 4 cut(s) 7, 9, 377, 418
BstHHI GCGC 4 cut(s) 7, 9, 379, 418
BstKTI GATC 1 cut(s) 870
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 1 cut(s) 867
BstNI CCWGG 2 cut(s) 176, 383
BstNSI RCATGY 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 174, 381
BstSFI CTRYAG 2 cut(s) 429, 662
BstUI CGCG 4 cut(s) 7, 9, 377, 418
BstV1I GCAGC 6 cut(s) 121, 287, 297, 454, 587, 648
BstV2I GAAGAC 3 cut(s) 229, 610, 655
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 3 cut(s) 138, 156, 445
BtgI CCRYGG 1 cut(s) 133
BtsCI GGATG 2 cut(s) 313, 880
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 285
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 3 cut(s) 7, 296, 420
CaiI CAGNNNCTG 1 cut(s) 310
CfoI GCGC 4 cut(s) 7, 9, 379, 418
Cfr10I RCCGGY 1 cut(s) 294
Cfr13I GGNCC 1 cut(s) 222
Csp6I GTAC 2 cut(s) 214, 302
CviAII CATG 2 cut(s) 332, 529
CviQI GTAC 2 cut(s) 214, 302
DdeI CTNAG 2 cut(s) 228, 440
DpnI GATC 1 cut(s) 869
DpnII GATC 1 cut(s) 867
EciI GGCGGA 2 cut(s) 39, 281
Eco130I CCWWGG 1 cut(s) 580
Eco32I GATATC 1 cut(s) 489
Eco47I GGWCC 1 cut(s) 222
EcoRII CCWGG 2 cut(s) 174, 381
EcoRV GATATC 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 580
ErhI CCWWGG 1 cut(s) 580
FaeI CATG 2 cut(s) 335, 532
FatI CATG 2 cut(s) 331, 528
FauI CCCGC 1 cut(s) 195
FauNDI CATATG 1 cut(s) 689
FokI GGATG 2 cut(s) 300, 867
GlaI GCGC 4 cut(s) 6, 8, 378, 417
HaeIII GGCC 3 cut(s) 138, 156, 445
HapII CCGG 2 cut(s) 209, 295
HhaI GCGC 4 cut(s) 7, 9, 379, 418
Hin1II CATG 2 cut(s) 335, 532
Hin6I GCGC 4 cut(s) 5, 7, 377, 416
HinP1I GCGC 4 cut(s) 5, 7, 377, 416
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 9 cut(s) 38, 74, 126, 187, 322, 352, 606, 638, 769
HpaII CCGG 2 cut(s) 209, 295
HphI GGTGA 2 cut(s) 64, 764
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 5 cut(s) 69, 192, 231, 486, 713
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 3 cut(s) 82, 164, 170
HpyAV CCTTC 2 cut(s) 226, 828
HpyCH4III ACNGT 1 cut(s) 594
HpyCH4IV ACGT 2 cut(s) 652, 678
HpyCH4V TGCA 6 cut(s) 313, 557, 575, 622, 664, 737
HpyF3I CTNAG 2 cut(s) 228, 440
HpySE526I ACGT 2 cut(s) 652, 678
Hsp92II CATG 2 cut(s) 335, 532
HspAI GCGC 4 cut(s) 5, 7, 377, 416
KroI GCCGGC 1 cut(s) 294
KroNI GCCGGC 1 cut(s) 296
Kzo9I GATC 1 cut(s) 867
LmnI GCTCC 1 cut(s) 384
Lsp1109I GCAGC 6 cut(s) 121, 287, 297, 454, 587, 648
LweI GCATC 2 cut(s) 322, 704
MaeII ACGT 2 cut(s) 652, 678
MaeIII GTNAC 1 cut(s) 679
MalI GATC 1 cut(s) 869
MboI GATC 1 cut(s) 867
MboII GAAGA 6 cut(s) 121, 229, 355, 615, 660, 813
MfeI CAATTG 1 cut(s) 182
MluCI AATT 4 cut(s) 182, 250, 402, 480
MlyI GAGTC 5 cut(s) 47, 68, 361, 600, 647
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 7 cut(s) 115, 149, 167, 435, 661, 837, 864
MroNI GCCGGC 1 cut(s) 294
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 1 cut(s) 788
MspI CCGG 2 cut(s) 209, 295
MspR9I CCNGG 2 cut(s) 176, 383
MunI CAATTG 1 cut(s) 182
MvaI CCWGG 2 cut(s) 176, 383
MvnI CGCG 4 cut(s) 7, 9, 377, 418
NaeI GCCGGC 1 cut(s) 296
NdeI CATATG 1 cut(s) 689
NdeII GATC 1 cut(s) 867
NgoMIV GCCGGC 1 cut(s) 294
NlaIII CATG 2 cut(s) 335, 532
NlaIV GGNNCC 1 cut(s) 207
NmeAIII GCCGAG 2 cut(s) 375, 471
NmuCI GTSAC 1 cut(s) 679
NspI RCATGY 1 cut(s) 335
PaqCI CACCTGC 1 cut(s) 548
PauI GCGCGC 1 cut(s) 5
PciI ACATGT 1 cut(s) 331
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PdiI GCCGGC 1 cut(s) 296
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 4 cut(s) 126, 187, 322, 769
PflMI CCANNNNNTGG 1 cut(s) 863
PleI GAGTC 5 cut(s) 46, 68, 360, 600, 646
PpsI GAGTC 5 cut(s) 46, 68, 360, 600, 646
Ppu21I YACGTR 1 cut(s) 679
PscI ACATGT 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 174, 381
PspGI CCWGG 2 cut(s) 174, 381
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 1 cut(s) 222
PstI CTGCAG 1 cut(s) 666
PstNI CAGNNNCTG 1 cut(s) 310
PteI GCGCGC 1 cut(s) 5
RsaI GTAC 2 cut(s) 215, 303
RsaNI GTAC 2 cut(s) 214, 302
SaqAI TTAA 1 cut(s) 788
Sau3AI GATC 1 cut(s) 867
Sau96I GGNCC 1 cut(s) 222
SchI GAGTC 5 cut(s) 47, 68, 361, 600, 647
ScrFI CCNGG 2 cut(s) 176, 383
SfaNI GCATC 2 cut(s) 322, 704
SfcI CTRYAG 2 cut(s) 429, 662
SinI GGWCC 1 cut(s) 222
Sse9I AATT 4 cut(s) 182, 250, 402, 480
StyD4I CCNGG 2 cut(s) 174, 381
StyI CCWWGG 1 cut(s) 580
TaaI ACNGT 1 cut(s) 594
TaiI ACGT 2 cut(s) 655, 681
TaqI TCGA 2 cut(s) 80, 271
TaqII GACCGA 1 cut(s) 138
TasI AATT 4 cut(s) 182, 250, 402, 480
TatI WGTACW 1 cut(s) 301
TauI GCSGC 8 cut(s) 12, 15, 18, 21, 24, 47, 50, 752
TfiI GAWTC 4 cut(s) 126, 187, 322, 769
Tru1I TTAA 1 cut(s) 788
Tru9I TTAA 1 cut(s) 788
TscAI CASTG 1 cut(s) 285
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 6 cut(s) 109, 275, 310, 467, 575, 661
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 2 cut(s) 274, 826
TspRI CASTG 1 cut(s) 285
Van91I CCANNNNNTGG 1 cut(s) 863
VpaK11BI GGWCC 1 cut(s) 222
XapI RAATTY 1 cut(s) 402
XceI RCATGY 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.