Rh4AG429400

Protein TOM THREE HOMOLOG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0006559
Physical Location & Seq
Forward (+)
72482019 .. 72488060
6042 bp
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UTR
Exon/CDS
Intron
Rh4AG429400.1

Sequence Viewer

Length: 789 bp
ATGGCGCGCGGCGGCGGCGGTATGGAGTCGGCGGCGGCGGTGATAGCTTACAATCTGAAAGACTCGTCGAGTTGGTGGCACGACATCAATGCCTCGCAGCTCTGGCAAGACCGAATCTTCCATGGCCTCGCCATTCTCTACGGCCTCGTCGCCGTCGTTGCCCTGGTTCAACTGATCCGGATACAAGTGCGGGTGCCGGAGTACGGCTGGACCACGCAGAAGGTCTTCCACTTTCTCAATTTTGTTGTCAATGCAGTTCGAGCAGCAGTGTTCATTTTCCGCCGGCAAGTACAGAAGCTGCATCCAGAGATTCTCCAACATGTATTGCTTGATATGCCGAGTCTGGCTTTCTTCACTACTTACGCGCTCCTGGTTCTGTTCTGGGCAGAAATTTACTATCAGGCGCGTGCTGTTTCTACAGATGGACTGAGGCCGAGCTTCTTTACAATCAATGCTGCCGTTTATGTAATTCAGATATCTATGTGGTTGATATTATGGTGGAAGCCTATCCCAGTCGTGGTCGTCTTATCTAAAATGTTCTTTGCAGGTGTCTCCTTATTTGCAGCCCTTGGATTTCTTCTGTATGGTGGAAGACTCTTTTTGATGTTGCAGAGATTTCCTGTGGAGTCAAAAGGAAGACGTAAGAAGCTGCAGGAGGTTGGCTACGTGACAACCATATGTTTTACTTGTTTCCTTGTCAGATGCGTAATGGTGAGTCTGCTTGAATCTTTTGGAGTTCATCTATACTACTCCTTGAACTTGATGTTTTTAAGCATGTCTAGGTTTTAA

Protein Analysis

262

Amino Acids

30.04

Weight (kDa)

9.8

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 22 - 240 1.9e-116 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 536
Acc36I ACCTGC 1 cut(s) 536
AccB1I GGYRCC 1 cut(s) 193
AccII CGCG 4 cut(s) 7, 9, 365, 406
AccIII TCCGGA 1 cut(s) 177
AciI CCGC 9 cut(s) 9, 12, 15, 18, 32, 35, 38, 190, 280
AclWI GGATC 1 cut(s) 169
AcsI RAATTY 1 cut(s) 390
AfaI GTAC 2 cut(s) 203, 291
AfiI CCNNNNNNNGG 2 cut(s) 203, 517
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 3 cut(s) 170, 725, 757
AjnI CCWGG 2 cut(s) 162, 369
AluBI AGCT 5 cut(s) 47, 100, 298, 438, 649
AluI AGCT 5 cut(s) 47, 100, 298, 438, 649
Alw26I GTCTC 1 cut(s) 556
AlwI GGATC 1 cut(s) 169
AlwNI CAGNNNCTG 1 cut(s) 298
Aor13HI TCCGGA 1 cut(s) 177
AoxI GGCC 3 cut(s) 124, 142, 431
ApeKI GCWGC 6 cut(s) 97, 263, 298, 455, 563, 649
ApoI RAATTY 1 cut(s) 390
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 4 cut(s) 7, 9, 367, 406
AspS9I GGNCC 1 cut(s) 210
AsuHPI GGTGA 2 cut(s) 52, 724
AvaII GGWCC 1 cut(s) 210
BanI GGYRCC 1 cut(s) 193
BbsI GAAGAC 3 cut(s) 217, 598, 643
BbvI GCAGC 6 cut(s) 109, 275, 285, 442, 575, 636
BccI CCATC 1 cut(s) 416
BceAI ACGGC 4 cut(s) 137, 157, 220, 443
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 2 cut(s) 164, 371
BciVI GTATCC 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 556
BfaI CTAG 1 cut(s) 780
BfmI CTRYAG 2 cut(s) 417, 650
BfuAI ACCTGC 1 cut(s) 536
BfuI GTATCC 1 cut(s) 174
Bme1390I CCNGG 2 cut(s) 164, 371
Bme18I GGWCC 1 cut(s) 210
BmgT120I GGNCC 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 2 cut(s) 164, 371
BmrI ACTGGG 1 cut(s) 506
BmsI GCATC 2 cut(s) 310, 692
BmuI ACTGGG 1 cut(s) 506
BpiI GAAGAC 3 cut(s) 217, 598, 643
BsaAI YACGTR 1 cut(s) 667
BsaJI CCNNGG 3 cut(s) 121, 162, 568
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 517
Bse118I RCCGGY 1 cut(s) 282
Bse1I ACTGG 1 cut(s) 512
BseAI TCCGGA 1 cut(s) 177
BseBI CCWGG 2 cut(s) 164, 371
BseDI CCNNGG 3 cut(s) 121, 162, 568
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 2 cut(s) 203, 517
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 1 cut(s) 512
BsePI GCGCGC 1 cut(s) 5
BseXI GCAGC 6 cut(s) 109, 275, 285, 442, 575, 636
Bsh1236I CGCG 4 cut(s) 7, 9, 365, 406
BshFI GGCC 3 cut(s) 126, 144, 433
BshNI GGYRCC 1 cut(s) 193
BsiSI CCGG 3 cut(s) 178, 197, 283
BslI CCNNNNNNNGG 2 cut(s) 203, 517
BsmAI GTCTC 1 cut(s) 556
BsnI GGCC 3 cut(s) 126, 144, 433
Bsp13I TCCGGA 1 cut(s) 177
Bsp143I GATC 1 cut(s) 174
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 9 cut(s) 9, 12, 15, 18, 32, 35, 38, 190, 280
BspANI GGCC 3 cut(s) 126, 144, 433
BspCNI CTCAG 1 cut(s) 420
BspEI TCCGGA 1 cut(s) 177
BspFNI CGCG 4 cut(s) 7, 9, 365, 406
BspLI GGNNCC 1 cut(s) 195
BspMAI CTGCAG 1 cut(s) 654
BspMI ACCTGC 1 cut(s) 536
BspPI GGATC 1 cut(s) 169
BspT107I GGYRCC 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 282
BsrI ACTGG 1 cut(s) 512
BssAI RCCGGY 1 cut(s) 282
BssECI CCNNGG 3 cut(s) 121, 162, 568
BssHII GCGCGC 1 cut(s) 5
BssMI GATC 1 cut(s) 174
BssT1I CCWWGG 2 cut(s) 121, 568
Bst2UI CCWGG 2 cut(s) 164, 371
BstBAI YACGTR 1 cut(s) 667
BstC8I GCNNGC 3 cut(s) 7, 284, 408
BstDEI CTNAG 1 cut(s) 428
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 4 cut(s) 7, 9, 365, 406
BstHHI GCGC 4 cut(s) 7, 9, 367, 406
BstKTI GATC 1 cut(s) 177
BstMAI GTCTC 1 cut(s) 556
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 6 cut(s) 15, 44, 103, 158, 260, 334
BstNI CCWGG 2 cut(s) 164, 371
BstNSI RCATGY 2 cut(s) 323, 778
BstSCI CCNGG 2 cut(s) 162, 369
BstSFI CTRYAG 2 cut(s) 417, 650
BstUI CGCG 4 cut(s) 7, 9, 365, 406
BstV1I GCAGC 6 cut(s) 109, 275, 285, 442, 575, 636
BstV2I GAAGAC 3 cut(s) 217, 598, 643
BsuI GTATCC 1 cut(s) 174
BsuRI GGCC 3 cut(s) 126, 144, 433
BtgI CCRYGG 1 cut(s) 121
BtsCI GGATG 1 cut(s) 301
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 273
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 3 cut(s) 7, 284, 408
CaiI CAGNNNCTG 1 cut(s) 298
CfoI GCGC 4 cut(s) 7, 9, 367, 406
Cfr10I RCCGGY 1 cut(s) 282
Cfr13I GGNCC 1 cut(s) 210
Csp6I GTAC 2 cut(s) 202, 290
CviAII CATG 3 cut(s) 122, 320, 775
CviQI GTAC 2 cut(s) 202, 290
DdeI CTNAG 1 cut(s) 428
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
EciI GGCGGA 1 cut(s) 269
Eco130I CCWWGG 2 cut(s) 121, 568
Eco32I GATATC 1 cut(s) 477
Eco47I GGWCC 1 cut(s) 210
EcoRII CCWGG 2 cut(s) 162, 369
EcoRV GATATC 1 cut(s) 477
EcoT14I CCWWGG 2 cut(s) 121, 568
ErhI CCWWGG 2 cut(s) 121, 568
FaeI CATG 3 cut(s) 125, 323, 778
FatI CATG 3 cut(s) 121, 319, 774
FauI CCCGC 1 cut(s) 183
FauNDI CATATG 1 cut(s) 677
FokI GGATG 1 cut(s) 288
FspBI CTAG 1 cut(s) 780
GlaI GCGC 4 cut(s) 6, 8, 366, 405
HaeIII GGCC 3 cut(s) 126, 144, 433
HapII CCGG 3 cut(s) 178, 197, 283
HhaI GCGC 4 cut(s) 7, 9, 367, 406
Hin1II CATG 3 cut(s) 125, 323, 778
Hin6I GCGC 4 cut(s) 5, 7, 365, 404
HinP1I GCGC 4 cut(s) 5, 7, 365, 404
HinfI GANTC 9 cut(s) 26, 62, 114, 310, 340, 594, 626, 715, 725
HpaII CCGG 3 cut(s) 178, 197, 283
HphI GGTGA 2 cut(s) 52, 724
Hpy188I TCNGA 3 cut(s) 57, 474, 701
Hpy188III TCNNGA 2 cut(s) 178, 305
Hpy99I CGWCG 3 cut(s) 70, 152, 158
HpyAV CCTTC 1 cut(s) 214
HpyCH4IV ACGT 2 cut(s) 640, 666
HpyCH4V TGCA 6 cut(s) 254, 301, 545, 563, 610, 652
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 44, 103, 158, 260, 334
HpyF3I CTNAG 1 cut(s) 428
HpySE526I ACGT 2 cut(s) 640, 666
Hsp92II CATG 3 cut(s) 125, 323, 778
HspAI GCGC 4 cut(s) 5, 7, 365, 404
Kpn2I TCCGGA 1 cut(s) 177
KroI GCCGGC 1 cut(s) 282
KroNI GCCGGC 1 cut(s) 284
Kzo9I GATC 1 cut(s) 174
LmnI GCTCC 1 cut(s) 372
Lsp1109I GCAGC 6 cut(s) 109, 275, 285, 442, 575, 636
LweI GCATC 2 cut(s) 310, 692
MaeI CTAG 1 cut(s) 780
MaeII ACGT 2 cut(s) 640, 666
MaeIII GTNAC 1 cut(s) 667
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MboII GAAGA 6 cut(s) 109, 217, 343, 569, 603, 648
MluCI AATT 3 cut(s) 238, 390, 468
MlyI GAGTC 6 cut(s) 35, 56, 349, 588, 635, 724
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 5 cut(s) 103, 137, 155, 423, 649
MroI TCCGGA 1 cut(s) 177
MroNI GCCGGC 1 cut(s) 282
MroXI GAANNNNTTC 1 cut(s) 224
MseI TTAA 2 cut(s) 770, 787
MspI CCGG 3 cut(s) 178, 197, 283
MspR9I CCNGG 2 cut(s) 164, 371
MvaI CCWGG 2 cut(s) 164, 371
MvnI CGCG 4 cut(s) 7, 9, 365, 406
MwoI GCNNNNNNNGC 6 cut(s) 15, 44, 103, 158, 260, 334
NaeI GCCGGC 1 cut(s) 284
NcoI CCATGG 1 cut(s) 121
NdeI CATATG 1 cut(s) 677
NdeII GATC 1 cut(s) 174
NgoMIV GCCGGC 1 cut(s) 282
NlaIII CATG 3 cut(s) 125, 323, 778
NlaIV GGNNCC 1 cut(s) 195
NmeAIII GCCGAG 2 cut(s) 363, 459
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 2 cut(s) 323, 778
PaqCI CACCTGC 1 cut(s) 536
PauI GCGCGC 1 cut(s) 5
PciI ACATGT 1 cut(s) 319
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdiI GCCGGC 1 cut(s) 284
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 3 cut(s) 114, 310, 725
PleI GAGTC 6 cut(s) 34, 56, 348, 588, 634, 723
PpsI GAGTC 6 cut(s) 34, 56, 348, 588, 634, 723
Ppu21I YACGTR 1 cut(s) 667
PscI ACATGT 1 cut(s) 319
Psp6I CCWGG 2 cut(s) 162, 369
PspGI CCWGG 2 cut(s) 162, 369
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 1 cut(s) 210
PstI CTGCAG 1 cut(s) 654
PstNI CAGNNNCTG 1 cut(s) 298
PteI GCGCGC 1 cut(s) 5
RsaI GTAC 2 cut(s) 203, 291
RsaNI GTAC 2 cut(s) 202, 290
SaqAI TTAA 2 cut(s) 770, 787
Sau3AI GATC 1 cut(s) 174
Sau96I GGNCC 1 cut(s) 210
SchI GAGTC 6 cut(s) 35, 56, 349, 588, 635, 724
ScrFI CCNGG 2 cut(s) 164, 371
SfaNI GCATC 2 cut(s) 310, 692
SfcI CTRYAG 2 cut(s) 417, 650
SinI GGWCC 1 cut(s) 210
Sse9I AATT 3 cut(s) 238, 390, 468
SsiI CCGC 9 cut(s) 9, 12, 15, 18, 32, 35, 38, 190, 280
SspMI CTAG 1 cut(s) 780
StyD4I CCNGG 2 cut(s) 162, 369
StyI CCWWGG 2 cut(s) 121, 568
TaiI ACGT 2 cut(s) 643, 669
TaqI TCGA 2 cut(s) 68, 259
TaqII GACCGA 1 cut(s) 126
TasI AATT 3 cut(s) 238, 390, 468
TatI WGTACW 1 cut(s) 289
TauI GCSGC 5 cut(s) 12, 15, 18, 35, 38
TfiI GAWTC 3 cut(s) 114, 310, 725
Tru1I TTAA 2 cut(s) 770, 787
Tru9I TTAA 2 cut(s) 770, 787
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 6 cut(s) 97, 263, 298, 455, 563, 649
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 2 cut(s) 262, 728
TspRI CASTG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 210
XapI RAATTY 1 cut(s) 390
XceI RCATGY 2 cut(s) 323, 778
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.