Rh5AG253900

Protein TOM THREE HOMOLOG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006559
Physical Location & Seq
Reverse (-)
32721848 .. 32723018
1171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG253900.1

Sequence Viewer

Length: 894 bp
ATGGGGCATAGCGTTGGTGTGGTGCAGTCGGCGGCGGCGGTGATAGCTTACAATCTCAGACAGTCGTCAAGTTGGTGGCACGACATCAATGCCTCGCTGCTCTGGCAAGACCGAATCATCCACGGCCTCGCCATTCTCTACGTCCTCGTCGCTTTCGTTGCCCTAGTTCAATTGGTTGGGACACAGGTGCGAGTGCCGGAGTACGGCTGGACCACCCAGAAGGTCTTCCAATTTCTCAATTTTATTGTCAATGCAGTTCGATCAGCAGTGTTTATTTTCCGCCGGCAAGTACAGAAGCTGCATCCAGAGATTCTCCAACATGTGTTGCTTGATATGCCAAGTCTGGCTTTCTTCACAACTTACGCGCTTCTGGTTCTGTTCTGGGCTGAAATCTACTATAAGGCGCGTGCTGTTTTTACAGATGGACTAAGGCCGAGCTTCTTTACAATCAACGCTGCTGTTTATCTAGTTCAGATATCTATGTGGTTGATATTATGGTGGAACCCTATCCCAGTCGTGGTCTTGTTATCTAAACTGTTCTTCGCAGGGGTCTCCTTATTTGCAGCCCTTGGATTTCTTGTGTATGGTGGAAGACTGTTTCTGATGTTGCAGAGATTTCCTGTGGAATCGAAAGCAAGACGTAAGAAGCTGCATGAGGTTGGCTACGTGACAACCATATGTTTTACGTGTTTCCTTGTGAGATGCGTAATGATGGGCTTATGTGCATTCGACAAGGCGGCAGACCTTGATGTTTTGAATCACCCAGTTCTAAACTTGATCTATTACCTGTTGGTGGAGATATCGCCTTCTTCTTTGGTTCTTTTCATTCTGAGGAAGTTGCCTCCAAAGATTGGGATCACACAATATCAACCTATTCGCGCTCTGAAGGTATGA

Protein Analysis

297

Amino Acids

33.9

Weight (kDa)

9.77

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 22 - 293 2.2e-136 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 851
AccII CGCG 3 cut(s) 365, 406, 879
AciI CCGC 5 cut(s) 32, 35, 38, 280, 737
AclWI GGATC 1 cut(s) 863
AfaI GTAC 2 cut(s) 203, 291
AfiI CCNNNNNNNGG 4 cut(s) 203, 517, 793, 851
AflIII ACRYGT 2 cut(s) 319, 686
AgsI TTSAA 2 cut(s) 170, 757
AluBI AGCT 4 cut(s) 47, 298, 438, 649
AluI AGCT 4 cut(s) 47, 298, 438, 649
Alw26I GTCTC 1 cut(s) 556
AlwI GGATC 1 cut(s) 863
AlwNI CAGNNNCTG 1 cut(s) 298
AoxI GGCC 2 cut(s) 124, 431
ApeKI GCWGC 5 cut(s) 97, 298, 455, 563, 649
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 3 cut(s) 367, 406, 881
AspS9I GGNCC 1 cut(s) 210
AsuHPI GGTGA 2 cut(s) 52, 752
AvaII GGWCC 1 cut(s) 210
BbsI GAAGAC 2 cut(s) 217, 598
BbvI GCAGC 5 cut(s) 84, 285, 442, 575, 636
BccI CCATC 2 cut(s) 416, 706
BceAI ACGGC 2 cut(s) 139, 220
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BcoDI GTCTC 1 cut(s) 556
BfaI CTAG 2 cut(s) 164, 467
BisI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
BlsI GCNGC 8 cut(s) 34, 37, 99, 300, 457, 565, 651, 739
Bme18I GGWCC 1 cut(s) 210
BmgT120I GGNCC 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 503
BmrI ACTGGG 2 cut(s) 506, 758
BmsI GCATC 2 cut(s) 310, 692
BmuI ACTGGG 2 cut(s) 506, 758
BoxI GACNNNNGTC 1 cut(s) 64
BpiI GAAGAC 2 cut(s) 217, 598
BsaAI YACGTR 2 cut(s) 667, 687
BsaBI GATNNNNATC 1 cut(s) 854
BsaI GGTCTC 1 cut(s) 556
BsaJI CCNNGG 2 cut(s) 121, 568
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 517, 793, 851
Bse118I RCCGGY 1 cut(s) 282
Bse1I ACTGG 2 cut(s) 512, 764
Bse8I GATNNNNATC 1 cut(s) 854
BseDI CCNNGG 2 cut(s) 121, 568
BseGI GGATG 2 cut(s) 117, 301
BseJI GATNNNNATC 1 cut(s) 854
BseLI CCNNNNNNNGG 4 cut(s) 203, 517, 793, 851
BseMII CTCAG 2 cut(s) 70, 821
BseNI ACTGG 2 cut(s) 512, 764
BseXI GCAGC 5 cut(s) 84, 285, 442, 575, 636
BsgI GTGCAG 1 cut(s) 44
Bsh1236I CGCG 3 cut(s) 365, 406, 879
BshFI GGCC 2 cut(s) 126, 433
BsiSI CCGG 2 cut(s) 197, 283
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 4 cut(s) 203, 517, 793, 851
BsmAI GTCTC 1 cut(s) 556
BsmFI GGGAC 1 cut(s) 193
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 2 cut(s) 126, 433
Bso31I GGTCTC 1 cut(s) 556
Bsp143I GATC 3 cut(s) 260, 777, 855
BspACI CCGC 5 cut(s) 32, 35, 38, 280, 737
BspANI GGCC 2 cut(s) 126, 433
BspCNI CTCAG 2 cut(s) 69, 822
BspFNI CGCG 3 cut(s) 365, 406, 879
BspLI GGNNCC 1 cut(s) 503
BspPI GGATC 1 cut(s) 863
BspTNI GGTCTC 1 cut(s) 556
BsrFI RCCGGY 1 cut(s) 282
BsrI ACTGG 2 cut(s) 512, 764
BssAI RCCGGY 1 cut(s) 282
BssECI CCNNGG 2 cut(s) 121, 568
BssMI GATC 3 cut(s) 260, 777, 855
BssT1I CCWWGG 1 cut(s) 568
Bst4CI ACNGT 3 cut(s) 63, 537, 597
BstBAI YACGTR 2 cut(s) 667, 687
BstC8I GCNNGC 2 cut(s) 284, 408
BstDEI CTNAG 3 cut(s) 56, 428, 830
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 2 cut(s) 117, 301
BstFNI CGCG 3 cut(s) 365, 406, 879
BstHHI GCGC 3 cut(s) 367, 406, 881
BstKTI GATC 3 cut(s) 263, 780, 858
BstMAI GTCTC 1 cut(s) 556
BstMBI GATC 3 cut(s) 260, 777, 855
BstMWI GCNNNNNNNGC 4 cut(s) 44, 103, 158, 334
BstNSI RCATGY 1 cut(s) 323
BstPAI GACNNNNGTC 1 cut(s) 64
BstUI CGCG 3 cut(s) 365, 406, 879
BstV1I GCAGC 5 cut(s) 84, 285, 442, 575, 636
BstV2I GAAGAC 2 cut(s) 217, 598
BsuRI GGCC 2 cut(s) 126, 433
BtgI CCRYGG 1 cut(s) 121
BtsCI GGATG 2 cut(s) 117, 301
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 2 cut(s) 284, 408
CaiI CAGNNNCTG 1 cut(s) 298
CfoI GCGC 3 cut(s) 367, 406, 881
Cfr10I RCCGGY 1 cut(s) 282
Cfr13I GGNCC 1 cut(s) 210
Csp6I GTAC 2 cut(s) 202, 290
CviAII CATG 2 cut(s) 320, 653
CviQI GTAC 2 cut(s) 202, 290
DdeI CTNAG 3 cut(s) 56, 428, 830
DpnI GATC 3 cut(s) 262, 779, 857
DpnII GATC 3 cut(s) 260, 777, 855
EciI GGCGGA 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 568
Eco31I GGTCTC 1 cut(s) 556
Eco32I GATATC 2 cut(s) 477, 801
Eco47I GGWCC 1 cut(s) 210
EcoRV GATATC 2 cut(s) 477, 801
EcoT14I CCWWGG 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 568
FaeI CATG 2 cut(s) 323, 656
FalI AAGNNNNNCTT 2 cut(s) 331, 363
FaqI GGGAC 1 cut(s) 193
FatI CATG 2 cut(s) 319, 652
FauNDI CATATG 1 cut(s) 677
Fnu4HI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
FokI GGATG 2 cut(s) 104, 288
Fsp4HI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
FspBI CTAG 2 cut(s) 164, 467
GlaI GCGC 3 cut(s) 366, 405, 880
GluI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
HaeIII GGCC 2 cut(s) 126, 433
HapII CCGG 2 cut(s) 197, 283
HhaI GCGC 3 cut(s) 367, 406, 881
Hin1II CATG 2 cut(s) 323, 656
Hin6I GCGC 3 cut(s) 365, 404, 879
HinP1I GCGC 3 cut(s) 365, 404, 879
HinfI GANTC 4 cut(s) 114, 310, 626, 757
HpaII CCGG 2 cut(s) 197, 283
HphI GGTGA 2 cut(s) 52, 752
Hpy188I TCNGA 5 cut(s) 59, 474, 603, 831, 885
Hpy188III TCNNGA 1 cut(s) 305
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 3 cut(s) 214, 816, 880
HpyCH4III ACNGT 3 cut(s) 63, 537, 597
HpyCH4IV ACGT 4 cut(s) 141, 640, 666, 686
HpyCH4V TGCA 7 cut(s) 25, 254, 301, 563, 610, 652, 725
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 103, 158, 334
HpyF3I CTNAG 3 cut(s) 56, 428, 830
HpySE526I ACGT 4 cut(s) 141, 640, 666, 686
Hsp92II CATG 2 cut(s) 323, 656
HspAI GCGC 3 cut(s) 365, 404, 879
KroI GCCGGC 1 cut(s) 282
KroNI GCCGGC 1 cut(s) 284
Kzo9I GATC 3 cut(s) 260, 777, 855
Lsp1109I GCAGC 5 cut(s) 84, 285, 442, 575, 636
LweI GCATC 2 cut(s) 310, 692
MaeI CTAG 2 cut(s) 164, 467
MaeII ACGT 4 cut(s) 141, 640, 666, 686
MaeIII GTNAC 1 cut(s) 667
MalI GATC 3 cut(s) 262, 779, 857
MboI GATC 3 cut(s) 260, 777, 855
MboII GAAGA 5 cut(s) 217, 343, 532, 603, 801
MfeI CAATTG 1 cut(s) 170
MluCI AATT 3 cut(s) 170, 230, 238
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 6 cut(s) 103, 137, 155, 649, 825, 852
MroNI GCCGGC 1 cut(s) 282
MroXI GAANNNNTTC 1 cut(s) 224
MspI CCGG 2 cut(s) 197, 283
MunI CAATTG 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 725
MvnI CGCG 3 cut(s) 365, 406, 879
MwoI GCNNNNNNNGC 4 cut(s) 44, 103, 158, 334
NaeI GCCGGC 1 cut(s) 284
NdeI CATATG 1 cut(s) 677
NdeII GATC 3 cut(s) 260, 777, 855
NgoMIV GCCGGC 1 cut(s) 282
NlaIII CATG 2 cut(s) 323, 656
NlaIV GGNNCC 1 cut(s) 503
NmeAIII GCCGAG 1 cut(s) 459
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 1 cut(s) 323
PciI ACATGT 1 cut(s) 319
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 725
PdiI GCCGGC 1 cut(s) 284
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 4 cut(s) 114, 310, 626, 757
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 8 cut(s) 34, 37, 99, 300, 457, 565, 651, 739
Ppu21I YACGTR 2 cut(s) 667, 687
PscI ACATGT 1 cut(s) 319
PshAI GACNNNNGTC 1 cut(s) 64
PspN4I GGNNCC 1 cut(s) 503
PspPI GGNCC 1 cut(s) 210
PstNI CAGNNNCTG 1 cut(s) 298
RsaI GTAC 2 cut(s) 203, 291
RsaNI GTAC 2 cut(s) 202, 290
SatI GCNGC 8 cut(s) 33, 36, 98, 299, 456, 564, 650, 738
Sau3AI GATC 3 cut(s) 260, 777, 855
Sau96I GGNCC 1 cut(s) 210
SfaNI GCATC 2 cut(s) 310, 692
SinI GGWCC 1 cut(s) 210
Sse9I AATT 3 cut(s) 170, 230, 238
SsiI CCGC 5 cut(s) 32, 35, 38, 280, 737
SspMI CTAG 2 cut(s) 164, 467
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 3 cut(s) 63, 537, 597
TaiI ACGT 4 cut(s) 144, 643, 669, 689
TaqI TCGA 3 cut(s) 259, 629, 729
TaqII GACCGA 1 cut(s) 126
TasI AATT 3 cut(s) 170, 230, 238
TatI WGTACW 1 cut(s) 289
TauI GCSGC 3 cut(s) 35, 38, 740
TfiI GAWTC 4 cut(s) 114, 310, 626, 757
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 5 cut(s) 97, 298, 455, 563, 649
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 1 cut(s) 814
TspRI CASTG 1 cut(s) 273
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 1 cut(s) 210
XceI RCATGY 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 2 cut(s) 164, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.