RchiOBHm_Chr5g0037351

Protein TOM THREE HOMOLOG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006559
Physical Location & Seq
Reverse (-)
31779807 .. 31780773
967 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31607

Sequence Viewer

Length: 873 bp
ATGGCGTTGGCGGCGTCGGTGATAGCTTACAATCTCAGACAGTCGTCAAGTTGGTGGCACGACATCAATGCCTCGCTGCTCTGGCAAGACCGAATCATCCACGGCCTCGCCATTCTCTACGTCCTCGTCGCTTTCGTTGCCCTAGTTCAATTGGTTGGGACACAGGTGCGAGTGCCGGAGTACGGCTGGACCACCCAGAAGGTCTTCCAATTTCTCAATTTTATTGTCAATGCAGTTCGATCAGCAGTGTTTATTTTCCGCCGGCAAGTACAGAAGCTGCATCCAGAGATTCTCCAACATGTGTTGCTTGATATGCCAAGTCTGGCTTTCTTCACAACTTACGCGCTTCTGGTTCTGTTCTGGGCTGAAATCTACTATAAGGCGCGTGCTGTTTTTACAGATGGACTAAGGCCGAGCTTCTTTACAATCAACGCTGCTGTTTATCTAGTTCAGATATCTATGTGGTTGATATTATGGTGGAACCCTATCCCAGTCGTGGTCTTGTTATCTAAACTGTTCTTCGCAGGGGTCTCCTTATTTGCAGCCCTTGGATTTCTTGTGTATGGTGGAAGACTGTTTCTGATGTTGCAGAGATTTCCTGTGGAATCGAAAGCAAGACGTAAGAAGCTGCATGAGGTTGGCTACGTGACAACCATATGTTTTACGTGTTTCCTTGTGAGATGCGTAATGATGGGCTTATGTGCATTCGACAAGGCGGCAGACCTTGATGTTTTGAATCACCCAGTTCTAAACTTGATCTATTACCTGTTGGTGGAGATATCGCCTTCTTCTTTGGTTCTTTTCATTCTGAGGAAGTTGCCTCCAAAGATTGGGATCACACAATATCAACCTATTCGCGCTCTGAAGGTATGA

Protein Analysis

290

Amino Acids

33.25

Weight (kDa)

9.77

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 15 - 286 2e-136 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 830
AccII CGCG 3 cut(s) 344, 385, 858
AciI CCGC 3 cut(s) 11, 259, 716
AclWI GGATC 1 cut(s) 842
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 2 cut(s) 182, 270
AfiI CCNNNNNNNGG 4 cut(s) 182, 496, 772, 830
AflIII ACRYGT 2 cut(s) 298, 665
AgsI TTSAA 2 cut(s) 149, 736
AluBI AGCT 4 cut(s) 26, 277, 417, 628
AluI AGCT 4 cut(s) 26, 277, 417, 628
Alw26I GTCTC 1 cut(s) 535
AlwI GGATC 1 cut(s) 842
AlwNI CAGNNNCTG 1 cut(s) 277
AoxI GGCC 2 cut(s) 103, 410
ApeKI GCWGC 5 cut(s) 76, 277, 434, 542, 628
Asp700I GAANNNNTTC 1 cut(s) 203
AspLEI GCGC 3 cut(s) 346, 385, 860
AspS9I GGNCC 1 cut(s) 189
AsuHPI GGTGA 2 cut(s) 31, 731
AvaII GGWCC 1 cut(s) 189
BbsI GAAGAC 2 cut(s) 196, 577
BbvI GCAGC 5 cut(s) 63, 264, 421, 554, 615
BccI CCATC 2 cut(s) 395, 685
BceAI ACGGC 2 cut(s) 118, 199
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BcoDI GTCTC 1 cut(s) 535
BfaI CTAG 2 cut(s) 143, 446
BisI GCNGC 7 cut(s) 12, 77, 278, 435, 543, 629, 717
BlsI GCNGC 7 cut(s) 13, 78, 279, 436, 544, 630, 718
Bme18I GGWCC 1 cut(s) 189
BmgT120I GGNCC 1 cut(s) 189
BmiI GGNNCC 1 cut(s) 482
BmrI ACTGGG 2 cut(s) 485, 737
BmsI GCATC 2 cut(s) 289, 671
BmuI ACTGGG 2 cut(s) 485, 737
BoxI GACNNNNGTC 1 cut(s) 43
BpiI GAAGAC 2 cut(s) 196, 577
BsaAI YACGTR 2 cut(s) 646, 666
BsaBI GATNNNNATC 1 cut(s) 833
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 2 cut(s) 100, 547
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 496, 772, 830
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 2 cut(s) 491, 743
Bse8I GATNNNNATC 1 cut(s) 833
BseDI CCNNGG 2 cut(s) 100, 547
BseGI GGATG 2 cut(s) 96, 280
BseJI GATNNNNATC 1 cut(s) 833
BseLI CCNNNNNNNGG 4 cut(s) 182, 496, 772, 830
BseMII CTCAG 2 cut(s) 49, 800
BseNI ACTGG 2 cut(s) 491, 743
BseXI GCAGC 5 cut(s) 63, 264, 421, 554, 615
Bsh1236I CGCG 3 cut(s) 344, 385, 858
BshFI GGCC 2 cut(s) 105, 412
BsiSI CCGG 2 cut(s) 176, 262
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 4 cut(s) 182, 496, 772, 830
BsmAI GTCTC 1 cut(s) 535
BsmFI GGGAC 1 cut(s) 172
BsmI GAATGC 1 cut(s) 704
BsnI GGCC 2 cut(s) 105, 412
Bso31I GGTCTC 1 cut(s) 535
Bsp143I GATC 3 cut(s) 239, 756, 834
BspACI CCGC 3 cut(s) 11, 259, 716
BspANI GGCC 2 cut(s) 105, 412
BspCNI CTCAG 2 cut(s) 48, 801
BspFNI CGCG 3 cut(s) 344, 385, 858
BspLI GGNNCC 1 cut(s) 482
BspPI GGATC 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 261
BsrI ACTGG 2 cut(s) 491, 743
BssAI RCCGGY 1 cut(s) 261
BssECI CCNNGG 2 cut(s) 100, 547
BssMI GATC 3 cut(s) 239, 756, 834
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 547
Bst4CI ACNGT 3 cut(s) 42, 516, 576
BstACI GRCGYC 1 cut(s) 14
BstBAI YACGTR 2 cut(s) 646, 666
BstC8I GCNNGC 2 cut(s) 263, 387
BstDEI CTNAG 3 cut(s) 35, 407, 809
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 2 cut(s) 96, 280
BstFNI CGCG 3 cut(s) 344, 385, 858
BstHHI GCGC 3 cut(s) 346, 385, 860
BstKTI GATC 3 cut(s) 242, 759, 837
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 3 cut(s) 239, 756, 834
BstMWI GCNNNNNNNGC 4 cut(s) 11, 82, 137, 313
BstNSI RCATGY 1 cut(s) 302
BstPAI GACNNNNGTC 1 cut(s) 43
BstUI CGCG 3 cut(s) 344, 385, 858
BstV1I GCAGC 5 cut(s) 63, 264, 421, 554, 615
BstV2I GAAGAC 2 cut(s) 196, 577
BsuRI GGCC 2 cut(s) 105, 412
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 2 cut(s) 96, 280
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 252
Cac8I GCNNGC 2 cut(s) 263, 387
CaiI CAGNNNCTG 1 cut(s) 277
CfoI GCGC 3 cut(s) 346, 385, 860
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 189
CseI GACGC 1 cut(s) 3
Csp6I GTAC 2 cut(s) 181, 269
CviAII CATG 2 cut(s) 299, 632
CviQI GTAC 2 cut(s) 181, 269
DdeI CTNAG 3 cut(s) 35, 407, 809
DpnI GATC 3 cut(s) 241, 758, 836
DpnII GATC 3 cut(s) 239, 756, 834
EciI GGCGGA 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 547
Eco31I GGTCTC 1 cut(s) 535
Eco32I GATATC 2 cut(s) 456, 780
Eco47I GGWCC 1 cut(s) 189
EcoRV GATATC 2 cut(s) 456, 780
EcoT14I CCWWGG 1 cut(s) 547
ErhI CCWWGG 1 cut(s) 547
FaeI CATG 2 cut(s) 302, 635
FalI AAGNNNNNCTT 2 cut(s) 310, 342
FaqI GGGAC 1 cut(s) 172
FatI CATG 2 cut(s) 298, 631
FauNDI CATATG 1 cut(s) 656
Fnu4HI GCNGC 7 cut(s) 12, 77, 278, 435, 543, 629, 717
FokI GGATG 2 cut(s) 83, 267
Fsp4HI GCNGC 7 cut(s) 12, 77, 278, 435, 543, 629, 717
FspBI CTAG 2 cut(s) 143, 446
GlaI GCGC 3 cut(s) 345, 384, 859
GluI GCNGC 7 cut(s) 12, 77, 278, 435, 543, 629, 717
HaeIII GGCC 2 cut(s) 105, 412
HapII CCGG 2 cut(s) 176, 262
HgaI GACGC 1 cut(s) 3
HhaI GCGC 3 cut(s) 346, 385, 860
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 2 cut(s) 302, 635
Hin6I GCGC 3 cut(s) 344, 383, 858
HinP1I GCGC 3 cut(s) 344, 383, 858
HinfI GANTC 4 cut(s) 93, 289, 605, 736
HpaII CCGG 2 cut(s) 176, 262
HphI GGTGA 2 cut(s) 31, 731
Hpy188I TCNGA 5 cut(s) 38, 453, 582, 810, 864
Hpy188III TCNNGA 1 cut(s) 284
Hpy99I CGWCG 2 cut(s) 19, 131
HpyAV CCTTC 3 cut(s) 193, 795, 859
HpyCH4III ACNGT 3 cut(s) 42, 516, 576
HpyCH4IV ACGT 4 cut(s) 120, 619, 645, 665
HpyCH4V TGCA 6 cut(s) 233, 280, 542, 589, 631, 704
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 82, 137, 313
HpyF3I CTNAG 3 cut(s) 35, 407, 809
HpySE526I ACGT 4 cut(s) 120, 619, 645, 665
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 2 cut(s) 302, 635
HspAI GCGC 3 cut(s) 344, 383, 858
KroI GCCGGC 1 cut(s) 261
KroNI GCCGGC 1 cut(s) 263
Kzo9I GATC 3 cut(s) 239, 756, 834
Lsp1109I GCAGC 5 cut(s) 63, 264, 421, 554, 615
LweI GCATC 2 cut(s) 289, 671
MaeI CTAG 2 cut(s) 143, 446
MaeII ACGT 4 cut(s) 120, 619, 645, 665
MaeIII GTNAC 1 cut(s) 646
MalI GATC 3 cut(s) 241, 758, 836
MboI GATC 3 cut(s) 239, 756, 834
MboII GAAGA 5 cut(s) 196, 322, 511, 582, 780
MfeI CAATTG 1 cut(s) 149
MluCI AATT 3 cut(s) 149, 209, 217
MmeI TCCRAC 1 cut(s) 319
MnlI CCTC 6 cut(s) 82, 116, 134, 628, 804, 831
MroNI GCCGGC 1 cut(s) 261
MroXI GAANNNNTTC 1 cut(s) 203
MspI CCGG 2 cut(s) 176, 262
MunI CAATTG 1 cut(s) 149
Mva1269I GAATGC 1 cut(s) 704
MvnI CGCG 3 cut(s) 344, 385, 858
MwoI GCNNNNNNNGC 4 cut(s) 11, 82, 137, 313
NaeI GCCGGC 1 cut(s) 263
NdeI CATATG 1 cut(s) 656
NdeII GATC 3 cut(s) 239, 756, 834
NgoMIV GCCGGC 1 cut(s) 261
NlaIII CATG 2 cut(s) 302, 635
NlaIV GGNNCC 1 cut(s) 482
NmeAIII GCCGAG 1 cut(s) 438
NmuCI GTSAC 1 cut(s) 646
NspI RCATGY 1 cut(s) 302
PciI ACATGT 1 cut(s) 298
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 1 cut(s) 704
PdiI GCCGGC 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 4 cut(s) 93, 289, 605, 736
PflMI CCANNNNNTGG 1 cut(s) 830
PkrI GCNGC 7 cut(s) 13, 78, 279, 436, 544, 630, 718
Ppu21I YACGTR 2 cut(s) 646, 666
PscI ACATGT 1 cut(s) 298
PshAI GACNNNNGTC 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 482
PspPI GGNCC 1 cut(s) 189
PstNI CAGNNNCTG 1 cut(s) 277
RsaI GTAC 2 cut(s) 182, 270
RsaNI GTAC 2 cut(s) 181, 269
SatI GCNGC 7 cut(s) 12, 77, 278, 435, 543, 629, 717
Sau3AI GATC 3 cut(s) 239, 756, 834
Sau96I GGNCC 1 cut(s) 189
SfaNI GCATC 2 cut(s) 289, 671
SinI GGWCC 1 cut(s) 189
Sse9I AATT 3 cut(s) 149, 209, 217
SsiI CCGC 3 cut(s) 11, 259, 716
SspMI CTAG 2 cut(s) 143, 446
StyI CCWWGG 1 cut(s) 547
TaaI ACNGT 3 cut(s) 42, 516, 576
TaiI ACGT 4 cut(s) 123, 622, 648, 668
TaqI TCGA 3 cut(s) 238, 608, 708
TaqII GACCGA 1 cut(s) 105
TasI AATT 3 cut(s) 149, 209, 217
TatI WGTACW 1 cut(s) 268
TauI GCSGC 2 cut(s) 14, 719
TfiI GAWTC 4 cut(s) 93, 289, 605, 736
TscAI CASTG 1 cut(s) 252
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 5 cut(s) 76, 277, 434, 542, 628
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 1 cut(s) 793
TspRI CASTG 1 cut(s) 252
Van91I CCANNNNNTGG 1 cut(s) 830
VpaK11BI GGWCC 1 cut(s) 189
XceI RCATGY 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 2 cut(s) 143, 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.