FvH4_5g09290

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004579
Physical Location & Seq
Reverse (-)
5346137 .. 5351913
5777 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g09290.t2

Sequence Viewer

Length: 2769 bp
ATGGCTAGGGTTTTGGTTCACTCTACTAATATTCCTGGTCTGGTTGCCGGGCAAAGGCTTGGTCAATCTAAAGGCTCTGGAAATGCTAGAAGGTCAACGAAGATGATGTGCTCTTTACGAACTCCTGCTTTGAGAATTAGCAGTTTCTCGGGACTCCGTAGTGTTAATGCGTTAGATATCATGGCGAGGCCTGGGCAAGACTTATATTCTAAGGTGGGAGTAGCTATCTCATCCCGGCGTCGAAAGGCCAGCAGATGTGTGCCTAAAGCTATGTTTGAGCGCTTCACGGAAAAGGCAATAAAGGTTATTATGCTTGCACAAGAGGAAGCAAGGCGACTTGGTCACAACTTCGTTGGTACTGAGCAGATACTCTTGGGTCTTATTGGTGAAGGAACTGGAATTGCTGCCAAGGTCCTTAAATCTATGGGAATCAATCTTAAAGATGCACGCGTTGAAGTGGAAAAGATCATTGGAAGGGGCAGTGGCTTTGTTGCTGTTGAAATCCCATTCACTCCTCGTGCAAAGCGAGTTCTGGAACTGTCGCTGGAGGAAGCTCGCCAACTAGGTCATAACTATATTGGATCTGAGCACTTGCTTCTGGGACTGCTTCGGGAAGGTGAGGGTGTGGCAGCTCGTGTTCTTGAGAATTTAGGTGCTGACCCCAGCAATATTCGCACACAGGTTATTCGTATGGTGGGTGAGAGCACAGAAGCTGTTGGTGTTGGAGCTGGACAGGGAACTAGTGGCAACAAGATGCCAACTCTAGAGGAGTATGGAACCAATTTGACTAAGCTAGCAGAGGAGGGAAAGCTGGATCCTGTTGTTGGAAGACAACCTCAAATTGAACGCGTTGTCCAAATTTTGGGCAGGCGAACAAAGAACAATCCCTGCCTTATTGGAGAGCCTGGCGTTGGGAAGACTGCAATTGCAGAAGGTCTTGCTCAACGAATTGCAAGTGGCGATGTGCCTGAAACAATAGAGGGAAAGAAGGTTATCACTCTGGATATGGGTCTTCTTGTTGCTGGTACTAAATACCGTGGAGAGTTTGAGGAAAGATTAAAGAAGCTAATGGAGGAAATCAAACAAAGTGATGAGATAATCTTGTTTATTGATGAGGTGCACACTCTAATTGGAGCAGGAGCAGCAGAAGGTGCAATTGATGCTGCAAATATATTAAAACCAGCCCTGGCAAGAGGTGAACTGCAGTGTATTGGAGCCACCACTCTTGATGAATACAGAAAACACATTGAGAAAGACCCTGCATTGGAAAGACGTTTTCAACCTGTTAAAGTTCCTGAACCAACTGTGGACGAAACTATACAGATTTTGCGAGGGCTCCGGGAGCGATATGAGATTCACCACAAACTCCGGTATACTGATGAAGCTCTGATTTCTGCTGCCCAGCTATCATACCAGTACATCAGTGATCGATTTCTACCTGATAAAGCAATCGACTTGATTGATGAAGCTGGATCTCGAGTTCGCCTTCGTCATGCACAGCTACCTGAAGAAGCTAGAGAACTTGACAAGGAACTGAGACAGATTACCAAGGAGAAGAATGAAGCTGTACGGAGCCAAGACTTTGAAAAGGCTGGGGAGTTGCGTGACAGAGAAATGGACCTTAAGACACAGATCACTGCTATAACAGAGAAAGGCAAAGAGATGAGCAAGGCTGAGAGTGAGGCAGGGGATGTAGGACCTTTGGTAACCGAGGTGGATATTCAACATATCGTGGCCTCTTGGACAGGTATTCCTGTGGACAAAGTGTCAGCTGATGAATCCGATCGCCTCCTCAAGATGGAAGAGACCCTCCATACGCGAGTTATTGGTCAGGATGAAGCAGTCAAGGCTATCAGTCGTGCTATTCGCCGAGCCCGTGTTGGACTCAAGAATCCTAATCGTCCAATTGCCAGTTTCATCTTTTCTGGTCCAACTGGTGTTGGAAAATCCGAACTCGCAAAAGCTTTGGCTGCATACTACTTTGGTTCTCAAGAAGCCATGATCCGTCTTGATATGAGTGAATTCATGGAAAGGCACACTGTTTCAAAGCTTATTGGTTCACCTCCTGGTTATGTTGGTTACACTGAGGGAGGTCAGTTGACTGAAGCTGTTAGACGCCGTCCTTACACTGTGGTGCTTTTTGATGAGATTGAGAAGGCTCATCCGGATGTCTTCAACATGATGCTTCAAATTCTTGAGGATGGAAGATTGACAGACAGTAAGGGCAGAACTGTAGATTTCAAGAACACACTTCTGATCATGACTTCTAATGTTGGAAGCAGTGTAATTGAGAAAGGAGGCCGCCGTATAGGATTTGACCTTGATTACGATGAGAAGGACAGCAGTTACAATCGCATCAAGAGCTTGGTGACAGAGGAGTTGAAACAGTACTTTAGACCAGAGTTCCTAAATAGGTTGGATGAAATGATCGTCTTCCGCCAGCTCACAAAGTTAGAGGTGAAGGAAATAGCAGATATAATGCTGAAGGAGGTTTTTGAAAGGCTGAAGACTAAAGAGATAGAGCTTCAAGTGACAGAGAGGTTCAGAGATAGGGTGGTTGATGAAGGATACAATCCAAGCTATGGGGCAAGGCCTCTAAGAAGAGCTATAATGAGACTTCTGGAGGATAGCATGGCTGAGAAGATGCTAGCAAGGGAGATCAAAGAGGGTGACTCCGTTATTGTGGATGTCGATTCTGATGGGAATGTAACTGTGCTCAATGGTAACAGTGGTTCTGCTGGATCTTTGCCAGAGCCTGTTCCCGTTTGA

Protein Analysis

923

Amino Acids

102.21

Weight (kDa)

6.59

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 94 - 206 5.4e-33 Clp repeat (R) N-terminal domain
AAA PF00004 265 - 377 6.3e-14 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 404 - 504 2.3e-34 AAA lid domain
UVR PF02151 477 - 512 3.8e-09 UvrB/uvrC motif
AAA_2 PF07724 602 - 776 1.3e-68 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 607 - 728 8.9e-12 AAA domain (dynein-related subfamily)
AAA PF00004 608 - 727 2.5e-07 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 783 - 863 1.9e-24 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 862, 2582
AccI GTMKAC 1 cut(s) 1373
AccII CGCG 3 cut(s) 450, 849, 1819
AccIII TCCGGA 1 cut(s) 2164
AciI CCGC 2 cut(s) 2302, 2437
AclWI GGATC 6 cut(s) 589, 809, 822, 1480, 1996, 2749
AcsI RAATTY 4 cut(s) 646, 858, 2021, 2190
AcuI CTGAAG 4 cut(s) 1527, 2124, 2504, 2525
AcyI GRCGYC 2 cut(s) 238, 2116
AfaI GTAC 5 cut(s) 358, 1027, 1418, 1569, 2390
AfeI AGCGCT 1 cut(s) 281
AfiI CCNNNNNNNGG 7 cut(s) 54, 824, 862, 911, 1264, 1798, 2582
AflII CTTAAG 1 cut(s) 1622
AflIII ACRYGT 2 cut(s) 448, 847
AhdI GACNNNNNGTC 1 cut(s) 1765
AhlI ACTAGT 1 cut(s) 740
AjnI CCWGG 5 cut(s) 34, 190, 904, 1185, 2065
AjuI GAANNNNNNNTTGG 2 cut(s) 453, 485
AleI CACNNNNGTG 1 cut(s) 2132
AloI GAACNNNNNNTCC 4 cut(s) 837, 869, 1464, 1496
Alw21I GWGCWC 5 cut(s) 113, 591, 707, 1122, 2718
Alw26I GTCTC 3 cut(s) 1531, 1799, 2608
Alw44I GTGCAC 1 cut(s) 1118
AlwI GGATC 6 cut(s) 589, 809, 822, 1480, 1996, 2749
AlwNI CAGNNNCTG 2 cut(s) 713, 2756
Ama87I CYCGRG 2 cut(s) 148, 1476
Aor13HI TCCGGA 1 cut(s) 2164
Aor51HI AGCGCT 1 cut(s) 281
AoxI GGCC 5 cut(s) 188, 246, 1734, 2299, 2591
ApaLI GTGCAC 1 cut(s) 1118
ApeKI GCWGC 6 cut(s) 404, 629, 1142, 1163, 1397, 1970
ApoI RAATTY 4 cut(s) 646, 858, 2021, 2190
ArsI GACNNNNNNTTYG 2 cut(s) 46, 78
AspLEI GCGC 1 cut(s) 282
AspS9I GGNCC 4 cut(s) 412, 1618, 1697, 1928
AsuC2I CCSGG 3 cut(s) 49, 235, 1340
AsuHPI GGTGA 9 cut(s) 398, 629, 710, 1208, 1349, 2052, 2380, 2470, 2681
AsuNHI GCTAGC 2 cut(s) 793, 2647
AvaI CYCGRG 2 cut(s) 148, 1476
AvaII GGWCC 4 cut(s) 412, 1618, 1697, 1928
BaeGI GKGCMC 1 cut(s) 1122
BamHI GGATCC 1 cut(s) 814
BanII GRGCYC 2 cut(s) 1338, 1876
BauI CACGAG 2 cut(s) 516, 633
BbsI GAAGAC 6 cut(s) 835, 923, 1004, 2164, 2425, 2513
Bbv12I GWGCWC 5 cut(s) 113, 591, 707, 1122, 2718
BbvI GCAGC 6 cut(s) 391, 641, 1150, 1154, 1384, 1957
BccI CCATC 3 cut(s) 1792, 2195, 2693
BceAI ACGGC 2 cut(s) 2103, 2289
BciT130I CCWGG 5 cut(s) 36, 192, 906, 1187, 2067
BciVI GTATCC 1 cut(s) 2561
BclI TGATCA 1 cut(s) 2256
BcnI CCSGG 3 cut(s) 49, 235, 1340
BcoDI GTCTC 3 cut(s) 1531, 1799, 2608
BcuI ACTAGT 1 cut(s) 740
BfaI CTAG 8 cut(s) 6, 87, 563, 741, 764, 794, 1515, 2648
BfmI CTRYAG 2 cut(s) 1202, 2232
BfoI RGCGCY 1 cut(s) 283
BfrI CTTAAG 1 cut(s) 1622
BfuI GTATCC 1 cut(s) 2561
BisI GCNGC 7 cut(s) 405, 630, 1143, 1164, 1398, 1971, 2302
BlsI GCNGC 7 cut(s) 406, 631, 1144, 1165, 1399, 1972, 2303
BmcAI AGTACT 1 cut(s) 2390
Bme1390I CCNGG 8 cut(s) 36, 49, 192, 235, 906, 1187, 1340, 2067
Bme18I GGWCC 4 cut(s) 412, 1618, 1697, 1928
BmeRI GACNNNNNGTC 1 cut(s) 1765
BmeT110I CYCGRG 2 cut(s) 148, 1476
BmgT120I GGNCC 4 cut(s) 412, 1618, 1697, 1928
BmiI GGNNCC 5 cut(s) 778, 816, 1216, 1337, 1574
BmrFI CCNGG 8 cut(s) 36, 49, 192, 235, 906, 1187, 1340, 2067
BmsI GCATC 6 cut(s) 433, 744, 1150, 2172, 2364, 2634
BmtI GCTAGC 2 cut(s) 797, 2651
BpiI GAAGAC 6 cut(s) 835, 923, 1004, 2164, 2425, 2513
BplI GAGNNNNNCTC 2 cut(s) 2657, 2689
BpmI CTGGAG 2 cut(s) 566, 2642
BpuEI CTTGAG 5 cut(s) 662, 1778, 1871, 1974, 2216
BpuMI CCSGG 3 cut(s) 49, 235, 1340
Bsa29I ATCGAT 1 cut(s) 1429
BsaHI GRCGYC 2 cut(s) 238, 2116
BsaI GGTCTC 1 cut(s) 1799
BsaJI CCNNGG 6 cut(s) 191, 408, 1036, 1185, 1548, 1710
BsaWI WCCGGW 2 cut(s) 1368, 2164
Bsc4I CCNNNNNNNGG 7 cut(s) 54, 824, 862, 911, 1264, 1798, 2582
Bse1I ACTGG 4 cut(s) 400, 1414, 1911, 1939
BseAI TCCGGA 1 cut(s) 2164
BseBI CCWGG 5 cut(s) 36, 192, 906, 1187, 2067
BseCI ATCGAT 1 cut(s) 1429
BseDI CCNNGG 6 cut(s) 191, 408, 1036, 1185, 1548, 1710
BseGI GGATG 8 cut(s) 230, 1696, 1840, 2161, 2173, 2206, 2425, 2692
BseLI CCNNNNNNNGG 7 cut(s) 54, 824, 862, 911, 1264, 1798, 2582
BseMII CTCAG 6 cut(s) 351, 576, 1526, 1665, 2076, 2628
BseNI ACTGG 4 cut(s) 400, 1414, 1911, 1939
BseRI GAGGAG 5 cut(s) 504, 782, 815, 1781, 2390
BseSI GKGCMC 1 cut(s) 1122
BseXI GCAGC 6 cut(s) 391, 641, 1150, 1154, 1384, 1957
BseYI CCCAGC 3 cut(s) 662, 1401, 1592
Bsh1236I CGCG 3 cut(s) 450, 849, 1819
Bsh1285I CGRYCG 1 cut(s) 1786
BshFI GGCC 5 cut(s) 190, 248, 1736, 2301, 2593
BshVI ATCGAT 1 cut(s) 1429
BsiEI CGRYCG 1 cut(s) 1786
BsiHKAI GWGCWC 5 cut(s) 113, 591, 707, 1122, 2718
BsiHKCI CYCGRG 2 cut(s) 148, 1476
BsiSI CCGG 5 cut(s) 48, 235, 1339, 1369, 2165
BslFI GGGAC 2 cut(s) 165, 615
BslI CCNNNNNNNGG 7 cut(s) 54, 824, 862, 911, 1264, 1798, 2582
BsmAI GTCTC 3 cut(s) 1531, 1799, 2608
BsmFI GGGAC 2 cut(s) 165, 615
BsnI GGCC 5 cut(s) 190, 248, 1736, 2301, 2593
Bso31I GGTCTC 1 cut(s) 1799
BsoBI CYCGRG 2 cut(s) 148, 1476
Bsp1286I GDGCHC 7 cut(s) 113, 591, 707, 1122, 1338, 1876, 2718
Bsp13I TCCGGA 1 cut(s) 2164
BspACI CCGC 2 cut(s) 2302, 2437
BspANI GGCC 5 cut(s) 190, 248, 1736, 2301, 2593
BspCNI CTCAG 6 cut(s) 352, 577, 1527, 1666, 2077, 2629
BspDI ATCGAT 1 cut(s) 1429
BspEI TCCGGA 1 cut(s) 2164
BspFNI CGCG 3 cut(s) 450, 849, 1819
BspHI TCATGA 1 cut(s) 2259
BspLI GGNNCC 5 cut(s) 778, 816, 1216, 1337, 1574
BspMAI CTGCAG 1 cut(s) 1206
BspOI GCTAGC 2 cut(s) 797, 2651
BspPI GGATC 6 cut(s) 589, 809, 822, 1480, 1996, 2749
BspQI GCTCTTC 1 cut(s) 2596
BspTI CTTAAG 1 cut(s) 1622
BspTNI GGTCTC 1 cut(s) 1799
BsrI ACTGG 4 cut(s) 400, 1414, 1911, 1939
BssECI CCNNGG 6 cut(s) 191, 408, 1036, 1185, 1548, 1710
BssNAI GTATAC 1 cut(s) 1374
BssNI GRCGYC 2 cut(s) 238, 2116
BssSI CACGAG 2 cut(s) 516, 633
BssT1I CCWWGG 2 cut(s) 408, 1548
Bst1107I GTATAC 1 cut(s) 1374
Bst2BI CACGAG 2 cut(s) 516, 633
Bst2UI CCWGG 5 cut(s) 36, 192, 906, 1187, 2067
Bst6I CTCTTC 2 cut(s) 1797, 2596
BstACI GRCGYC 2 cut(s) 238, 2116
BstAFI CTTAAG 1 cut(s) 1622
BstAPI GCANNNNNTGC 2 cut(s) 1151, 1160
BstC8I GCNNGC 8 cut(s) 250, 315, 448, 556, 795, 869, 2441, 2649
BstDEI CTNAG 9 cut(s) 210, 360, 585, 789, 1535, 1674, 2085, 2597, 2637
BstDSI CCRYGG 1 cut(s) 1036
BstEII GGTNACC 1 cut(s) 1705
BstF5I GGATG 8 cut(s) 230, 1696, 1840, 2161, 2173, 2206, 2425, 2692
BstFNI CGCG 3 cut(s) 450, 849, 1819
BstH2I RGCGCY 1 cut(s) 283
BstHHI GCGC 1 cut(s) 282
BstMAI GTCTC 3 cut(s) 1531, 1799, 2608
BstMCI CGRYCG 1 cut(s) 1786
BstMWI GCNNNNNNNGC 8 cut(s) 672, 1142, 1151, 1160, 1342, 1847, 1970, 2361
BstNI CCWGG 5 cut(s) 36, 192, 906, 1187, 2067
BstPI GGTNACC 1 cut(s) 1705
BstSCI CCNGG 8 cut(s) 34, 47, 190, 233, 904, 1185, 1338, 2065
BstSFI CTRYAG 2 cut(s) 1202, 2232
BstSLI GKGCMC 1 cut(s) 1122
BstUI CGCG 3 cut(s) 450, 849, 1819
BstV1I GCAGC 6 cut(s) 391, 641, 1150, 1154, 1384, 1957
BstV2I GAAGAC 6 cut(s) 835, 923, 1004, 2164, 2425, 2513
BstX2I RGATCY 4 cut(s) 581, 814, 1472, 2741
BstYI RGATCY 4 cut(s) 581, 814, 1472, 2741
BstZ17I GTATAC 1 cut(s) 1374
Bsu15I ATCGAT 1 cut(s) 1429
BsuI GTATCC 1 cut(s) 2561
BsuRI GGCC 5 cut(s) 190, 248, 1736, 2301, 2593
BsuTUI ATCGAT 1 cut(s) 1429
BtgI CCRYGG 1 cut(s) 1036
BtgZI GCGATG 1 cut(s) 975
BtsCI GGATG 8 cut(s) 230, 1696, 1840, 2161, 2173, 2206, 2425, 2692
BtsI GCAGTG 4 cut(s) 487, 1211, 1635, 2287
BtsIMutI CAGTG 9 cut(s) 487, 1211, 1429, 1635, 2037, 2082, 2127, 2287, 2734
Cac8I GCNNGC 8 cut(s) 250, 315, 448, 556, 795, 869, 2441, 2649
CaiI CAGNNNCTG 2 cut(s) 713, 2756
CciI TCATGA 1 cut(s) 2259
CfoI GCGC 1 cut(s) 282
Cfr13I GGNCC 4 cut(s) 412, 1618, 1697, 1928
ClaI ATCGAT 1 cut(s) 1429
CseI GACGC 2 cut(s) 227, 2124
Csp6I GTAC 5 cut(s) 357, 1026, 1417, 1568, 2389
CspCI CAANNNNNGTGG 2 cut(s) 1207, 1242
CviAII CATG 7 cut(s) 181, 1493, 1999, 2026, 2179, 2260, 2632
CviQI GTAC 5 cut(s) 357, 1026, 1417, 1568, 2389
DdeI CTNAG 9 cut(s) 210, 360, 585, 789, 1535, 1674, 2085, 2597, 2637
DriI GACNNNNNGTC 1 cut(s) 1765
Eam1104I CTCTTC 2 cut(s) 1797, 2596
Eam1105I GACNNNNNGTC 1 cut(s) 1765
EarI CTCTTC 2 cut(s) 1797, 2596
EciI GGCGGA 1 cut(s) 2426
Eco130I CCWWGG 2 cut(s) 408, 1548
Eco147I AGGCCT 2 cut(s) 190, 2593
Eco24I GRGCYC 2 cut(s) 1338, 1876
Eco31I GGTCTC 1 cut(s) 1799
Eco32I GATATC 1 cut(s) 178
Eco47I GGWCC 4 cut(s) 412, 1618, 1697, 1928
Eco47III AGCGCT 1 cut(s) 281
Eco57I CTGAAG 4 cut(s) 1527, 2124, 2504, 2525
Eco88I CYCGRG 2 cut(s) 148, 1476
Eco91I GGTNACC 1 cut(s) 1705
EcoO109I RGGNCCY 2 cut(s) 412, 1697
EcoO65I GGTNACC 1 cut(s) 1705
EcoRI GAATTC 1 cut(s) 2021
EcoRII CCWGG 5 cut(s) 34, 190, 904, 1185, 2065
EcoRV GATATC 1 cut(s) 178
EcoT14I CCWWGG 2 cut(s) 408, 1548
EcoT38I GRGCYC 2 cut(s) 1338, 1876
ErhI CCWWGG 2 cut(s) 408, 1548
FaeI CATG 7 cut(s) 184, 1496, 2002, 2029, 2182, 2263, 2635
FaqI GGGAC 2 cut(s) 165, 615
FatI CATG 7 cut(s) 180, 1492, 1998, 2025, 2178, 2259, 2631
FbaI TGATCA 1 cut(s) 2256
FblI GTMKAC 1 cut(s) 1373
Fnu4HI GCNGC 7 cut(s) 405, 630, 1143, 1164, 1398, 1971, 2302
FokI GGATG 8 cut(s) 217, 1703, 1847, 2148, 2180, 2213, 2432, 2699
FriOI GRGCYC 2 cut(s) 1338, 1876
Fsp4HI GCNGC 7 cut(s) 405, 630, 1143, 1164, 1398, 1971, 2302
FspBI CTAG 8 cut(s) 6, 87, 563, 741, 764, 794, 1515, 2648
GlaI GCGC 1 cut(s) 281
GluI GCNGC 7 cut(s) 405, 630, 1143, 1164, 1398, 1971, 2302
GsaI CCCAGC 3 cut(s) 666, 1405, 1596
GsuI CTGGAG 2 cut(s) 566, 2642
HaeII RGCGCY 1 cut(s) 283
HaeIII GGCC 5 cut(s) 190, 248, 1736, 2301, 2593
HapII CCGG 5 cut(s) 48, 235, 1339, 1369, 2165
HgaI GACGC 2 cut(s) 227, 2124
HhaI GCGC 1 cut(s) 282
Hin1I GRCGYC 2 cut(s) 238, 2116
Hin1II CATG 7 cut(s) 184, 1496, 2002, 2029, 2182, 2263, 2635
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HincII GTYRAC 2 cut(s) 96, 2100
HindII GTYRAC 2 cut(s) 96, 2100
HindIII AAGCTT 2 cut(s) 1962, 2048
HinfI GANTC 8 cut(s) 153, 429, 1354, 1778, 1884, 1891, 2672, 2693
HpaII CCGG 5 cut(s) 48, 235, 1339, 1369, 2165
HphI GGTGA 9 cut(s) 398, 629, 710, 1208, 1349, 2052, 2380, 2470, 2681
Hpy166II GTNNAC 9 cut(s) 19, 96, 1120, 1199, 1309, 1374, 1759, 2060, 2100
Hpy188I TCNGA 7 cut(s) 586, 1389, 1783, 1951, 2256, 2546, 2698
Hpy8I GTNNAC 9 cut(s) 19, 96, 1120, 1199, 1309, 1374, 1759, 2060, 2100
Hpy99I CGWCG 1 cut(s) 243
HpyCH4IV ACGT 1 cut(s) 1273
HpyF10VI GCNNNNNNNGC 8 cut(s) 672, 1142, 1151, 1160, 1342, 1847, 1970, 2361
HpyF3I CTNAG 9 cut(s) 210, 360, 585, 789, 1535, 1674, 2085, 2597, 2637
HpySE526I ACGT 1 cut(s) 1273
Hsp92I GRCGYC 2 cut(s) 238, 2116
Hsp92II CATG 7 cut(s) 184, 1496, 2002, 2029, 2182, 2263, 2635
HspAI GCGC 1 cut(s) 280
Kpn2I TCCGGA 1 cut(s) 2164
Ksp22I TGATCA 1 cut(s) 2256
LguI GCTCTTC 1 cut(s) 2596
LmnI GCTCC 7 cut(s) 725, 1133, 1139, 1214, 1341, 1342, 1572
Lsp1109I GCAGC 6 cut(s) 391, 641, 1150, 1154, 1384, 1957
LweI GCATC 6 cut(s) 433, 744, 1150, 2172, 2364, 2634
MaeI CTAG 8 cut(s) 6, 87, 563, 741, 764, 794, 1515, 2648
MaeII ACGT 1 cut(s) 1273
MfeI CAATTG 3 cut(s) 924, 1155, 1905
MflI RGATCY 4 cut(s) 581, 814, 1472, 2741
MhlI GDGCHC 7 cut(s) 113, 591, 707, 1122, 1338, 1876, 2718
MluI ACGCGT 2 cut(s) 448, 847
MlyI GAGTC 3 cut(s) 147, 1878, 2666
MmeI TCCRAC 7 cut(s) 703, 805, 1861, 1921, 1955, 2254, 2397
MroI TCCGGA 1 cut(s) 2164
MseI TTAA 7 cut(s) 165, 417, 438, 1058, 1175, 1287, 1623
MslI CAYNNNNRTG 2 cut(s) 2132, 2166
MspA1I CMGCKG 1 cut(s) 1772
MspCI CTTAAG 1 cut(s) 1622
MspI CCGG 5 cut(s) 48, 235, 1339, 1369, 2165
MspR9I CCNGG 8 cut(s) 36, 49, 192, 235, 906, 1187, 1340, 2067
MunI CAATTG 3 cut(s) 924, 1155, 1905
MvaI CCWGG 5 cut(s) 36, 192, 906, 1187, 2067
MvnI CGCG 3 cut(s) 450, 849, 1819
MwoI GCNNNNNNNGC 8 cut(s) 672, 1142, 1151, 1160, 1342, 1847, 1970, 2361
NciI CCSGG 3 cut(s) 49, 235, 1340
NheI GCTAGC 2 cut(s) 793, 2647
NlaIII CATG 7 cut(s) 184, 1496, 2002, 2029, 2182, 2263, 2635
NlaIV GGNNCC 5 cut(s) 778, 816, 1216, 1337, 1574
NmeAIII GCCGAG 1 cut(s) 1895
NmuCI GTSAC 5 cut(s) 341, 1604, 2368, 2530, 2669
OliI CACNNNNGTG 1 cut(s) 2132
PaeR7I CTCGAG 1 cut(s) 1476
PagI TCATGA 1 cut(s) 2259
PceI AGGCCT 2 cut(s) 190, 2593
PciSI GCTCTTC 1 cut(s) 2596
PcsI WCGNNNNNNNCGW 2 cut(s) 523, 1873
PfeI GAWTC 5 cut(s) 429, 1354, 1778, 1891, 2693
PflFI GACNNNGTC 2 cut(s) 339, 2118
PflMI CCANNNNNTGG 2 cut(s) 862, 2582
PfoI TCCNGGA 1 cut(s) 1338
PkrI GCNGC 7 cut(s) 406, 631, 1144, 1165, 1399, 1972, 2303
Ple19I CGATCG 1 cut(s) 1786
PleI GAGTC 3 cut(s) 147, 1878, 2666
PpsI GAGTC 3 cut(s) 147, 1878, 2666
PpuMI RGGWCCY 2 cut(s) 412, 1697
Psp5II RGGWCCY 2 cut(s) 412, 1697
Psp6I CCWGG 5 cut(s) 34, 190, 904, 1185, 2065
PspEI GGTNACC 1 cut(s) 1705
PspFI CCCAGC 3 cut(s) 662, 1401, 1592
PspGI CCWGG 5 cut(s) 34, 190, 904, 1185, 2065
PspN4I GGNNCC 5 cut(s) 778, 816, 1216, 1337, 1574
PspPI GGNCC 4 cut(s) 412, 1618, 1697, 1928
PspPPI RGGWCCY 2 cut(s) 412, 1697
PsrI GAACNNNNNNTAC 2 cut(s) 2716, 2748
PstI CTGCAG 1 cut(s) 1206
PstNI CAGNNNCTG 2 cut(s) 713, 2756
PsuI RGATCY 4 cut(s) 581, 814, 1472, 2741
PsyI GACNNNGTC 2 cut(s) 339, 2118
PvuI CGATCG 1 cut(s) 1786
PvuII CAGCTG 1 cut(s) 1772
RsaI GTAC 5 cut(s) 358, 1027, 1418, 1569, 2390
RsaNI GTAC 5 cut(s) 357, 1026, 1417, 1568, 2389
RseI CAYNNNNRTG 2 cut(s) 2132, 2166
SapI GCTCTTC 1 cut(s) 2596
SaqAI TTAA 7 cut(s) 165, 417, 438, 1058, 1175, 1287, 1623
SatI GCNGC 7 cut(s) 405, 630, 1143, 1164, 1398, 1971, 2302
Sau96I GGNCC 4 cut(s) 412, 1618, 1697, 1928
ScaI AGTACT 1 cut(s) 2390
SchI GAGTC 3 cut(s) 147, 1878, 2666
ScrFI CCNGG 8 cut(s) 36, 49, 192, 235, 906, 1187, 1340, 2067
SduI GDGCHC 7 cut(s) 113, 591, 707, 1122, 1338, 1876, 2718
SfaNI GCATC 6 cut(s) 433, 744, 1150, 2172, 2364, 2634
SfcI CTRYAG 2 cut(s) 1202, 2232
Sfr274I CTCGAG 1 cut(s) 1476
SinI GGWCC 4 cut(s) 412, 1618, 1697, 1928
SlaI CTCGAG 1 cut(s) 1476
SmiMI CAYNNNNRTG 2 cut(s) 2132, 2166
SmlI CTYRAG 7 cut(s) 641, 1476, 1622, 1793, 1886, 1989, 2195
SmoI CTYRAG 7 cut(s) 641, 1476, 1622, 1793, 1886, 1989, 2195
SpeI ACTAGT 1 cut(s) 740
SseBI AGGCCT 2 cut(s) 190, 2593
SsiI CCGC 2 cut(s) 2302, 2437
SspI AATATT 2 cut(s) 31, 670
SspMI CTAG 8 cut(s) 6, 87, 563, 741, 764, 794, 1515, 2648
StuI AGGCCT 2 cut(s) 190, 2593
StyD4I CCNGG 8 cut(s) 34, 47, 190, 233, 904, 1185, 1338, 2065
StyI CCWWGG 2 cut(s) 408, 1548
TaiI ACGT 1 cut(s) 1276
TaqI TCGA 5 cut(s) 241, 1429, 1452, 1477, 2691
TatI WGTACW 2 cut(s) 1416, 2388
TauI GCSGC 1 cut(s) 2304
TfiI GAWTC 5 cut(s) 429, 1354, 1778, 1891, 2693
Tru1I TTAA 7 cut(s) 165, 417, 438, 1058, 1175, 1287, 1623
Tru9I TTAA 7 cut(s) 165, 417, 438, 1058, 1175, 1287, 1623
TscAI CASTG 9 cut(s) 487, 1211, 1429, 1642, 2044, 2089, 2134, 2287, 2734
TseFI GTSAC 5 cut(s) 341, 1604, 2368, 2530, 2669
TseI GCWGC 6 cut(s) 404, 629, 1142, 1163, 1397, 1970
Tsp45I GTSAC 5 cut(s) 341, 1604, 2368, 2530, 2669
TspGWI ACGGA 5 cut(s) 146, 302, 1585, 1994, 2665
TspRI CASTG 9 cut(s) 487, 1211, 1429, 1642, 2044, 2089, 2134, 2287, 2734
Tth111I GACNNNGTC 2 cut(s) 339, 2118
Van91I CCANNNNNTGG 2 cut(s) 862, 2582
Vha464I CTTAAG 1 cut(s) 1622
VneI GTGCAC 1 cut(s) 1118
VpaK11BI GGWCC 4 cut(s) 412, 1618, 1697, 1928
XapI RAATTY 4 cut(s) 646, 858, 2021, 2190
XbaI TCTAGA 1 cut(s) 763
XhoI CTCGAG 1 cut(s) 1476
XmiI GTMKAC 1 cut(s) 1373
XspI CTAG 8 cut(s) 6, 87, 563, 741, 764, 794, 1515, 2648
ZrmI AGTACT 1 cut(s) 2390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.