RchiOBHm_Chr7g0228651

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004579
Physical Location & Seq
Forward (+)
52021528 .. 52023804
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20482

Sequence Viewer

Length: 1200 bp
ATGGCTTGTTGCGAATCCAAGTATCCAATTCCACACATTGAGAGAGACCCAGCATTGGAAAGACGTTTTCAACAAGTCAAAGTTCCTGAACCATCTGTGGATGATACAATACAGATTCTGCGAGGGCTCCGGGAGCTATATGAGATCCACCACAAGCTCCGGTATACTGATGAAGCTCTGATAGCTGCCGCTCAGCTATCAGACCACTACATCAGTGATCGCTTTCTACCTGATAAAGCAATTGACTTGATTGATCGAGCTGGTTCTGAAGTTCGCTTTTGTCATGCACAGCTACCTGAAGAAACTAGAGATCTTGACAAAGAACTGAGACAGATTACCAAGGAGAAGAATGAAGCTGTTCGAAGCCAAGACTTTGAAAAGGTTGGGGAGTTACGTGAGAGAGAAATGGACCTTAAAACACAGATCAGAAATATAAAAGAGAAAGGAAAAGAGATGAGCAAGGCTGAGAGCGAGGCAGGGGATGTAGGTCCTATGGTAACTGAAGTGGATATTCAACGTATCGTGTTCTCTTGGATTGGTATTCCTGTTGACAAAGTGTCAGCTGATGAATCGGATCGCCTTCTCAAGATGGAAGAGACCCTCCATAAGCGGGTTATTGGTCAGGATGAAGCAGTCAAGGCTATTAGTCGTGCTATTCGCCGAGGCCGTGTTGGACTCAGATATCCTAATCGTCCAATTGCCAGTTTCATCTTTTCTGGTCCAACTGGTGTTGGAAAATCCGAACTCGCAAAAGCTTTGGCTGCATACTACTTTGGCTCTGAAGAAGCCATGATTTGCCTTGATATGAGTGAATTTATGGAACGGCACTCTGTTTCAAAGCTTATTGGTTCTCCCCCTGGTTATGTTGGTTACACTGATGGAGGTCAGCTGACTGAAGCCGTTAGACGCCGTCCTTACACTGTGGTGCTATTTGATGAGATTGAGAAGGCTGATCCAGATGTCTTAAATGTGATGCTTCAAATTCTTGAGGATGGAAGATTGACCGACAGCAAGGGCAGAACTGTGGATTTCAAGAATACACTTCTGATCATGACATCTAATGTCGGAAGCAGTGTAATTGAGAAAGGAGGACTCCGTGTAGGAGTTGATCTTGATTACGATGAGAATGATACCAGTCACAATCGCATCAAGAGCTCAGTGACAGAGGAGCTAAAACGCAATCTAGACCTGAGTTCCTAA

Protein Analysis

399

Amino Acids

45.14

Weight (kDa)

5.37

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_lid_9 PF17871 32 - 133 3.8e-33 AAA lid domain
AAA_2 PF07724 231 - 395 2.4e-59 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 236 - 357 4.2e-12 AAA domain (dynein-related subfamily)
AAA PF00004 237 - 355 3.4e-06 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 191
AccI GTMKAC 1 cut(s) 164
AciI CCGC 2 cut(s) 189, 610
AclWI GGATC 3 cut(s) 139, 582, 947
AcsI RAATTY 2 cut(s) 812, 981
AcuI CTGAAG 5 cut(s) 288, 318, 522, 801, 915
AcyI GRCGYC 1 cut(s) 907
AfiI CCNNNNNNNGG 2 cut(s) 55, 610
AgsI TTSAA 6 cut(s) 71, 377, 515, 837, 980, 1033
AhdI GACNNNNNGTC 1 cut(s) 556
AjnI CCWGG 1 cut(s) 856
AleI CACNNNNGTG 1 cut(s) 923
Alw21I GWGCWC 1 cut(s) 1157
Alw26I GTCTC 3 cut(s) 39, 322, 590
AlwI GGATC 3 cut(s) 139, 582, 947
AlwNI CAGNNNCTG 1 cut(s) 118
AoxI GGCC 1 cut(s) 664
ApeKI GCWGC 2 cut(s) 185, 761
ApoI RAATTY 2 cut(s) 812, 981
Asp700I GAANNNNTTC 1 cut(s) 357
AspS9I GGNCC 3 cut(s) 409, 488, 719
AsuC2I CCSGG 1 cut(s) 131
AsuII TTCGAA 1 cut(s) 361
AvaII GGWCC 3 cut(s) 409, 488, 719
BanII GRGCYC 2 cut(s) 129, 1157
Bbv12I GWGCWC 1 cut(s) 1157
BbvI GCAGC 2 cut(s) 172, 748
BccI CCATC 4 cut(s) 100, 583, 872, 986
BceAI ACGGC 4 cut(s) 651, 839, 884, 894
BciT130I CCWGG 1 cut(s) 858
BciVI GTATCC 1 cut(s) 33
BclI TGATCA 1 cut(s) 1047
BcnI CCSGG 1 cut(s) 131
BcoDI GTCTC 3 cut(s) 39, 322, 590
BfaI CTAG 2 cut(s) 306, 1184
BfuI GTATCC 1 cut(s) 33
BglII AGATCT 1 cut(s) 310
BisI GCNGC 3 cut(s) 186, 189, 762
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 3 cut(s) 187, 190, 763
Bme1390I CCNGG 2 cut(s) 131, 858
Bme18I GGWCC 3 cut(s) 409, 488, 719
BmeRI GACNNNNNGTC 1 cut(s) 556
BmgT120I GGNCC 3 cut(s) 409, 488, 719
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 2 cut(s) 131, 858
BmsI GCATC 2 cut(s) 963, 1155
Bpu1102I GCTNAGC 1 cut(s) 192
Bpu14I TTCGAA 1 cut(s) 361
BpuEI CTTGAG 2 cut(s) 569, 1007
BpuMI CCSGG 1 cut(s) 131
BsaAI YACGTR 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 907
BsaI GGTCTC 2 cut(s) 39, 590
BsaJI CCNNGG 3 cut(s) 339, 661, 856
BsaWI WCCGGW 1 cut(s) 159
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 610
Bse1I ACTGG 3 cut(s) 702, 730, 1134
BseBI CCWGG 1 cut(s) 858
BseDI CCNNGG 3 cut(s) 339, 661, 856
BseGI GGATG 4 cut(s) 106, 487, 631, 997
BseLI CCNNNNNNNGG 2 cut(s) 55, 610
BseMII CTCAG 6 cut(s) 206, 317, 456, 691, 1170, 1181
BseNI ACTGG 3 cut(s) 702, 730, 1134
BseRI GAGGAG 1 cut(s) 1181
BseXI GCAGC 2 cut(s) 172, 748
BseYI CCCAGC 1 cut(s) 49
BshFI GGCC 1 cut(s) 666
BsiHKAI GWGCWC 1 cut(s) 1157
BsiSI CCGG 2 cut(s) 130, 160
BslI CCNNNNNNNGG 2 cut(s) 55, 610
BsmAI GTCTC 3 cut(s) 39, 322, 590
BsnI GGCC 1 cut(s) 666
Bso31I GGTCTC 2 cut(s) 39, 590
Bsp119I TTCGAA 1 cut(s) 361
Bsp1286I GDGCHC 2 cut(s) 129, 1157
Bsp143I GATC 9 cut(s) 144, 217, 253, 310, 423, 574, 952, 1047, 1108
Bsp1720I GCTNAGC 1 cut(s) 192
BspACI CCGC 2 cut(s) 189, 610
BspANI GGCC 1 cut(s) 666
BspCNI CTCAG 6 cut(s) 205, 318, 457, 690, 1169, 1182
BspHI TCATGA 1 cut(s) 1050
BspLI GGNNCC 1 cut(s) 128
BspPI GGATC 3 cut(s) 139, 582, 947
BspT104I TTCGAA 1 cut(s) 361
BspTNI GGTCTC 2 cut(s) 39, 590
BsrBI CCGCTC 1 cut(s) 191
BsrI ACTGG 3 cut(s) 702, 730, 1134
BssECI CCNNGG 3 cut(s) 339, 661, 856
BssMI GATC 9 cut(s) 144, 217, 253, 310, 423, 574, 952, 1047, 1108
BssNAI GTATAC 1 cut(s) 165
BssNI GRCGYC 1 cut(s) 907
BssT1I CCWWGG 1 cut(s) 339
Bst1107I GTATAC 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 858
Bst4CI ACNGT 2 cut(s) 922, 1024
Bst6I CTCTTC 1 cut(s) 588
BstACI GRCGYC 1 cut(s) 907
BstBAI YACGTR 1 cut(s) 395
BstBI TTCGAA 1 cut(s) 361
BstDEI CTNAG 6 cut(s) 192, 326, 465, 677, 1156, 1190
BstF5I GGATG 4 cut(s) 106, 487, 631, 997
BstKTI GATC 9 cut(s) 147, 220, 256, 313, 426, 577, 955, 1050, 1111
BstMAI GTCTC 3 cut(s) 39, 322, 590
BstMBI GATC 9 cut(s) 144, 217, 253, 310, 423, 574, 952, 1047, 1108
BstMWI GCNNNNNNNGC 5 cut(s) 133, 182, 638, 761, 1152
BstNI CCWGG 1 cut(s) 858
BstSCI CCNGG 2 cut(s) 129, 856
BstV1I GCAGC 2 cut(s) 172, 748
BstX2I RGATCY 2 cut(s) 144, 310
BstYI RGATCY 2 cut(s) 144, 310
BstZ17I GTATAC 1 cut(s) 165
BsuI GTATCC 1 cut(s) 33
BsuRI GGCC 1 cut(s) 666
BtsCI GGATG 4 cut(s) 106, 487, 631, 997
BtsI GCAGTG 1 cut(s) 1078
BtsIMutI CAGTG 5 cut(s) 220, 873, 918, 1078, 1164
CaiI CAGNNNCTG 1 cut(s) 118
CciI TCATGA 1 cut(s) 1050
Cfr13I GGNCC 3 cut(s) 409, 488, 719
CseI GACGC 1 cut(s) 915
CviAII CATG 3 cut(s) 284, 790, 1051
DdeI CTNAG 6 cut(s) 192, 326, 465, 677, 1156, 1190
DpnI GATC 9 cut(s) 146, 219, 255, 312, 425, 576, 954, 1049, 1110
DpnII GATC 9 cut(s) 144, 217, 253, 310, 423, 574, 952, 1047, 1108
DriI GACNNNNNGTC 1 cut(s) 556
Eam1104I CTCTTC 1 cut(s) 588
Eam1105I GACNNNNNGTC 1 cut(s) 556
EarI CTCTTC 1 cut(s) 588
Ecl136II GAGCTC 1 cut(s) 1155
Eco130I CCWWGG 1 cut(s) 339
Eco24I GRGCYC 2 cut(s) 129, 1157
Eco31I GGTCTC 2 cut(s) 39, 590
Eco32I GATATC 1 cut(s) 683
Eco47I GGWCC 3 cut(s) 409, 488, 719
Eco53kI GAGCTC 1 cut(s) 1155
Eco57I CTGAAG 5 cut(s) 288, 318, 522, 801, 915
EcoICRI GAGCTC 1 cut(s) 1155
EcoO109I RGGNCCY 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 856
EcoRV GATATC 1 cut(s) 683
EcoT14I CCWWGG 1 cut(s) 339
EcoT38I GRGCYC 2 cut(s) 129, 1157
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 3 cut(s) 287, 793, 1054
FatI CATG 3 cut(s) 283, 789, 1050
FauI CCCGC 1 cut(s) 603
FbaI TGATCA 1 cut(s) 1047
FblI GTMKAC 1 cut(s) 164
Fnu4HI GCNGC 3 cut(s) 186, 189, 762
FokI GGATG 4 cut(s) 113, 494, 638, 1004
FriOI GRGCYC 2 cut(s) 129, 1157
Fsp4HI GCNGC 3 cut(s) 186, 189, 762
FspBI CTAG 2 cut(s) 306, 1184
GluI GCNGC 3 cut(s) 186, 189, 762
GsaI CCCAGC 1 cut(s) 53
HaeIII GGCC 1 cut(s) 666
HapII CCGG 2 cut(s) 130, 160
HgaI GACGC 1 cut(s) 915
Hin1I GRCGYC 1 cut(s) 907
Hin1II CATG 3 cut(s) 287, 793, 1054
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 2 cut(s) 753, 839
HinfI GANTC 5 cut(s) 14, 115, 569, 675, 1092
HpaII CCGG 2 cut(s) 130, 160
Hpy166II GTNNAC 2 cut(s) 165, 550
Hpy8I GTNNAC 2 cut(s) 165, 550
HpyAV CCTTC 2 cut(s) 590, 940
HpyCH4III ACNGT 2 cut(s) 922, 1024
HpyCH4IV ACGT 3 cut(s) 64, 394, 517
HpyCH4V TGCA 2 cut(s) 287, 764
HpyF10VI GCNNNNNNNGC 5 cut(s) 133, 182, 638, 761, 1152
HpyF3I CTNAG 6 cut(s) 192, 326, 465, 677, 1156, 1190
HpySE526I ACGT 3 cut(s) 64, 394, 517
Hsp92I GRCGYC 1 cut(s) 907
Hsp92II CATG 3 cut(s) 287, 793, 1054
Ksp22I TGATCA 1 cut(s) 1047
Kzo9I GATC 9 cut(s) 144, 217, 253, 310, 423, 574, 952, 1047, 1108
LmnI GCTCC 4 cut(s) 132, 133, 162, 1168
Lsp1109I GCAGC 2 cut(s) 172, 748
LweI GCATC 2 cut(s) 963, 1155
MaeI CTAG 2 cut(s) 306, 1184
MaeII ACGT 3 cut(s) 64, 394, 517
MaeIII GTNAC 5 cut(s) 390, 496, 869, 1136, 1159
MalI GATC 9 cut(s) 146, 219, 255, 312, 425, 576, 954, 1049, 1110
MbiI CCGCTC 1 cut(s) 191
MboI GATC 9 cut(s) 144, 217, 253, 310, 423, 574, 952, 1047, 1108
MboII GAAGA 5 cut(s) 311, 358, 605, 794, 1008
MfeI CAATTG 2 cut(s) 240, 696
MflI RGATCY 2 cut(s) 144, 310
MhlI GDGCHC 2 cut(s) 129, 1157
MluCI AATT 6 cut(s) 27, 240, 696, 812, 981, 1077
MlyI GAGTC 2 cut(s) 669, 1086
MmeI TCCRAC 4 cut(s) 652, 712, 746, 1045
MnlI CCTC 8 cut(s) 116, 466, 611, 656, 875, 982, 1082, 1159
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 2 cut(s) 414, 965
MslI CAYNNNNRTG 1 cut(s) 923
MspA1I CMGCKG 2 cut(s) 563, 889
MspI CCGG 2 cut(s) 130, 160
MspR9I CCNGG 2 cut(s) 131, 858
MunI CAATTG 2 cut(s) 240, 696
MvaI CCWGG 1 cut(s) 858
MwoI GCNNNNNNNGC 5 cut(s) 133, 182, 638, 761, 1152
NciI CCSGG 1 cut(s) 131
NdeII GATC 9 cut(s) 144, 217, 253, 310, 423, 574, 952, 1047, 1108
NlaIII CATG 3 cut(s) 287, 793, 1054
NlaIV GGNNCC 1 cut(s) 128
NmeAIII GCCGAG 1 cut(s) 686
NmuCI GTSAC 2 cut(s) 1136, 1159
NspV TTCGAA 1 cut(s) 361
OliI CACNNNNGTG 1 cut(s) 923
PagI TCATGA 1 cut(s) 1050
PcsI WCGNNNNNNNCGW 1 cut(s) 664
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 3 cut(s) 14, 115, 569
PflFI GACNNNGTC 1 cut(s) 909
PfoI TCCNGGA 1 cut(s) 129
PkrI GCNGC 3 cut(s) 187, 190, 763
PleI GAGTC 2 cut(s) 669, 1086
PpsI GAGTC 2 cut(s) 669, 1086
Ppu21I YACGTR 1 cut(s) 395
PpuMI RGGWCCY 1 cut(s) 488
Psp124BI GAGCTC 1 cut(s) 1157
Psp5II RGGWCCY 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 856
PspFI CCCAGC 1 cut(s) 49
PspGI CCWGG 1 cut(s) 856
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 3 cut(s) 409, 488, 719
PspPPI RGGWCCY 1 cut(s) 488
PstNI CAGNNNCTG 1 cut(s) 118
PsuI RGATCY 2 cut(s) 144, 310
PsyI GACNNNGTC 1 cut(s) 909
PvuII CAGCTG 2 cut(s) 563, 889
RseI CAYNNNNRTG 1 cut(s) 923
SacI GAGCTC 1 cut(s) 1157
SaqAI TTAA 2 cut(s) 414, 965
SatI GCNGC 3 cut(s) 186, 189, 762
Sau3AI GATC 9 cut(s) 144, 217, 253, 310, 423, 574, 952, 1047, 1108
Sau96I GGNCC 3 cut(s) 409, 488, 719
SchI GAGTC 2 cut(s) 669, 1086
ScrFI CCNGG 2 cut(s) 131, 858
SduI GDGCHC 2 cut(s) 129, 1157
SfaNI GCATC 2 cut(s) 963, 1155
SfuI TTCGAA 1 cut(s) 361
SinI GGWCC 3 cut(s) 409, 488, 719
SmiMI CAYNNNNRTG 1 cut(s) 923
SmlI CTYRAG 2 cut(s) 584, 986
SmoI CTYRAG 2 cut(s) 584, 986
Sse9I AATT 6 cut(s) 27, 240, 696, 812, 981, 1077
SsiI CCGC 2 cut(s) 189, 610
SspMI CTAG 2 cut(s) 306, 1184
SstI GAGCTC 1 cut(s) 1157
StyD4I CCNGG 2 cut(s) 129, 856
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 2 cut(s) 922, 1024
TaiI ACGT 3 cut(s) 67, 397, 520
TaqI TCGA 2 cut(s) 256, 361
TaqII GACCGA 1 cut(s) 1019
TasI AATT 6 cut(s) 27, 240, 696, 812, 981, 1077
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 3 cut(s) 14, 115, 569
Tru1I TTAA 2 cut(s) 414, 965
Tru9I TTAA 2 cut(s) 414, 965
TscAI CASTG 5 cut(s) 220, 880, 925, 1078, 1164
TseFI GTSAC 2 cut(s) 1136, 1159
TseI GCWGC 2 cut(s) 185, 761
Tsp45I GTSAC 2 cut(s) 1136, 1159
TspDTI ATGAA 5 cut(s) 186, 366, 582, 642, 697
TspGWI ACGGA 1 cut(s) 1085
TspRI CASTG 5 cut(s) 220, 880, 925, 1078, 1164
Tth111I GACNNNGTC 1 cut(s) 909
VpaK11BI GGWCC 3 cut(s) 409, 488, 719
XapI RAATTY 2 cut(s) 812, 981
XbaI TCTAGA 1 cut(s) 1183
XmiI GTMKAC 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 2 cut(s) 306, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.