MD14G1148700.v1.1

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004579
Physical Location & Seq
Forward (+)
24148751 .. 24154216
5466 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1148700.v1.1.491

Sequence Viewer

Length: 2721 bp
ATGGGGAGAAGGCTTGGCTACTCTAAAGGATCTGGGAATGCTAGAAGGACAGTCATGTTGTGTACTTTACGAGCACCTGCATTGAGAATGAGCGGTTACACAGGACTTCGGAGTGTTAATGCTTTAGATATTAGTGTAAAGCCGAGGCGAGACTTATTTTCTTCTGTGAAATTTGCAAACACTAAACGGCGTGAACGAGCTAGCAGATGTGTGCCTAAAGCCATGTTTGAGCGCTTCACAGAAAAAGCAATTAAAGTAATTATGCTTGCACAAGAGGAAGCAAGGCGACTAGGTCACAATTTTGTTGGCACGGAGCAGATACTTTTGGGTCTTATTGGTGAAGGGACTGGGATTGCTGCCAAGGTTCTCAAGTCCATGGGAATTAATCTTAAAGATGCACGAGTTGAAGTGGAGAAGATAATTGGAAGGGGTAGTGGATTTGTTGCTGTTGAAATTCCATTCACTCCTCGTGCAAAGCGAGTTTTGGAGCTGTCACTGGAGGAAGCTCGCCAACTTGGTCATAACTATATTGGATCCGAACATTTGCTTCTGGGTTTGCTTCGGGAGGGTGAGGGTGTAGCAGCTCGTGTTCTTGAGAATTTAGGTGCTGACCCTAGTAACATTCGCACACAGGTTATTCGTATGGTCGGTGAGAGCACAGAAGCTGTTGGTGCTGGTGTTGGAGGAGGGACCAGTGGTAATAAAATGCCAACTCTAGAAGAATATGGGACCAATTTGACTAAGCTAGCAGAGGAGGGTAAGTTGGATCCTGTTGTTGGAAGACAACAGCAAATTGAACGTGTCGTCCAAATTTTGGGTAGGAGGACTAAGAATAATCCCTGCCTTATTGGAGAACCTGGTGTTGGGAAGACTGCAATTGCAGAAGGTCTTGCTCAACGGATTTCAACTGGTGATGTTCCTGAAACAATAGAGGGAAAGAAGGTTATAACTCTGGATATGGGTCTTCTTGTTGCCGGTACAAAGTACCGTGGAGAGTTTGAGGAAAGATTGAAGAAGCTAATGGAGGAAATCAAACAAAGTGATGAGATTATTTTGTTTATTGATGAGGTGCACACTTTAATTGGAGCAGGAGCAGCAGAAGGGGCAATTGATGCTGCAAATATATTAAAACCGGCTCTTGCAAGAGGTGAACTGCAGTGCATTGGAGCCACAACTCTCGATGAGTACAGAAAGCACATTGAGAAAGATCCAGCTTTGGAAAGGCGATTCCAACCAGTCAAAGTTCCTGAACCAACCGTGGATGAAACCATTCAGATTTTGAGAGGGCTTAGGGAGCGGTATGAGATTCACCACAAGCTCCGCTACACTGATGAAGCCTTGGTATCTGCTGCACAGCTATCATACCAGTACATCAGTGATCGTTTTTTGCCTGATAAGGCAATTGACTTGGTTGATGAAGCTGGTTCTCGAGTTCGCCTTCGTCATGCACAGCTACCTGAAGAAGCCAGAGGGATTGAGAAAGAGCTGAGGGCGATCACTAAAGAGAAGAACGAGGCTGTTAGAAGCCAAGACTTTGAAAAGGCGGGGGAGTTACGTGACAAAGAAATGGACCTAAAGGCACAGATCACAGCTGTTGTTGATAAAGGCAAGGAGATGAGCAAGGCAGAGAGTGAGGCAGGAGATGTAGGTCCTCTCGTGACTGAAGTGGATATTCAACATATTGTGTCCTCCTGGACTGGAATTCCAGTTGAGAAAGTGTCAACCGATGAATCAGACCGCCTCCTCAAGATGGAAGAGACCCTCCATAAGCGAGTTATTGGTCAGGATGAAGCAGTCAAGGCTATTAGTCGTGCTATTCGCCGAGCCCGTGTTGGACTAAAGAATCCTAATCGTCCTATTGCCAGTTTCATCTTTTCTGGTCCCACTGGTGTTGGGAAATCTGAGCTCGCTAAAGCTTTGGCTGCTTACTACTTTGGTTCTGAAGAAGCCATGATTCGGCTTGATATGAGTGAGTTCATGGAAAGGCACACTGTTTCCAAGCTCATTGGTTCTCCCCCGGGTTATGTTGGTTACACTGAGGGAGGTCAGCTGACTGAAGCTGTTCGACGCCGTCCTTACACTGTAGTGCTTTTTGATGAAATTGAGAAGGCTCATCCCGATGTCTTCAACATGATGCTCCAAATACTCGAGGATGGAAGATTAACCGACAGCAAGGGCAGAACCGTGGATTTCAAGAATACACTTCTGATAATGACATCTAATGTTGGAAGCAGTGTGATCGAGAAAGGAGGCCGACGCATTGGATTTGACCTTGATTATGATGAGAAGGATAGCAGTTACAATAGAATTAAGAGCTTGGTGACGGAGGAACTAAAACAGTACTTCAGACCAGAGTTCTTGAATAGGTTGGACGAAATGATTGTTTTCCGACAACTCACCAAGCTGGAGGTGAAGGACATAGCAGATATAATGCTCAAGGAGGTTTTCGAGAGGTTGAAGAAGAAAGAGATAGAGCTTCAAGTGACAGAGAGGTTCAGAGATAGAGTGGTTGACGAAGGATACAACCCGAGCTACGGAGCCAGGCCTCTAAGAAGAGCTATCATGAGACTCTTGGAGGACAGCATGGCTGAGAAGATGCTTGCAAGGGAGATCAAAGAAGGTGACTCGGTCATTGTGGATGTCGATTCTGATGGAAACGTGACCGTGCTCAACGGCAGCAGCGGTTCTCCCGAGTCCTTGCCAGAGCCGATTCCTGTTTGA

Protein Analysis

907

Amino Acids

100.76

Weight (kDa)

6.73

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 78 - 200 1.4e-36 Clp repeat (R) N-terminal domain
Clp_N PF02861 154 - 224 1.6e-16 Clp repeat (R) N-terminal domain
AAA PF00004 281 - 393 5.6e-14 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 420 - 521 5.8e-34 AAA lid domain
UVR PF02151 493 - 528 9.8e-08 UvrB/uvrC motif
AAA_2 PF07724 618 - 792 9.5e-69 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 623 - 744 1.1e-11 AAA domain (dynein-related subfamily)
AAA PF00004 624 - 743 3.2e-07 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 799 - 879 2e-24 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 947
AarI CACCTGC 1 cut(s) 85
Acc36I ACCTGC 1 cut(s) 85
AccB7I CCANNNNNTGG 1 cut(s) 814
AccBSI CCGCTC 2 cut(s) 93, 1297
AciI CCGC 6 cut(s) 93, 1297, 1321, 1544, 1738, 2682
AclWI GGATC 6 cut(s) 37, 528, 541, 761, 774, 1202
AcsI RAATTY 5 cut(s) 170, 453, 598, 810, 1701
AcuI CTGAAG 5 cut(s) 1479, 1683, 1962, 2076, 2329
AcyI GRCGYC 1 cut(s) 2068
AfaI GTAC 6 cut(s) 64, 979, 986, 1187, 1370, 2342
AfeI AGCGCT 1 cut(s) 233
AfiI CCNNNNNNNGG 6 cut(s) 776, 814, 863, 1750, 1956, 2534
AflIII ACRYGT 1 cut(s) 799
AjnI CCWGG 3 cut(s) 856, 1691, 2540
AjuI GAANNNNNNNTTGG 2 cut(s) 846, 878
AleI CACNNNNGTG 1 cut(s) 2084
AloI GAACNNNNNNTCC 2 cut(s) 789, 821
Alw21I GWGCWC 5 cut(s) 76, 659, 1074, 1908, 2670
Alw26I GTCTC 3 cut(s) 144, 1751, 2560
Alw44I GTGCAC 1 cut(s) 1070
AlwI GGATC 6 cut(s) 37, 528, 541, 761, 774, 1202
AlwNI CAGNNNCTG 1 cut(s) 665
Ama87I CYCGRG 5 cut(s) 1428, 2017, 2147, 2527, 2690
Aor51HI AGCGCT 1 cut(s) 233
AoxI GGCC 2 cut(s) 2251, 2543
ApaLI GTGCAC 1 cut(s) 1070
ApeKI GCWGC 8 cut(s) 356, 581, 1094, 1115, 1349, 1922, 2676, 2679
ApoI RAATTY 5 cut(s) 170, 453, 598, 810, 1701
AseI ATTAAT 1 cut(s) 384
Asp700I GAANNNNTTC 2 cut(s) 1269, 2061
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 5 cut(s) 690, 729, 1570, 1649, 1880
AsuC2I CCSGG 2 cut(s) 2018, 2019
AsuNHI GCTAGC 2 cut(s) 200, 745
AvaI CYCGRG 5 cut(s) 1428, 2017, 2147, 2527, 2690
AvaII GGWCC 5 cut(s) 690, 729, 1570, 1649, 1880
BaeGI GKGCMC 1 cut(s) 1074
BamHI GGATCC 2 cut(s) 533, 766
BanII GRGCYC 2 cut(s) 1828, 1908
BauI CACGAG 4 cut(s) 399, 468, 585, 1655
BbsI GAAGAC 4 cut(s) 787, 875, 956, 2116
Bbv12I GWGCWC 5 cut(s) 76, 659, 1074, 1908, 2670
BbvCI CCTCAGC 1 cut(s) 1487
BbvI GCAGC 8 cut(s) 343, 593, 1102, 1106, 1336, 1909, 2688, 2691
BccI CCATC 3 cut(s) 1744, 2147, 2645
BceAI ACGGC 3 cut(s) 203, 2055, 2689
BcgI CGANNNNNNTGC 2 cut(s) 2221, 2255
BciT130I CCWGG 3 cut(s) 858, 1693, 2542
BciVI GTATCC 1 cut(s) 2513
BcnI CCSGG 2 cut(s) 2018, 2019
BcoDI GTCTC 3 cut(s) 144, 1751, 2560
BfaI CTAG 6 cut(s) 42, 201, 290, 615, 716, 746
BfmI CTRYAG 2 cut(s) 1154, 2082
BfoI RGCGCY 1 cut(s) 235
BfuAI ACCTGC 1 cut(s) 85
BfuI GTATCC 1 cut(s) 2513
BisI GCNGC 8 cut(s) 357, 582, 1095, 1116, 1350, 1923, 2677, 2680
BlsI GCNGC 8 cut(s) 358, 583, 1096, 1117, 1351, 1924, 2678, 2681
BmcAI AGTACT 1 cut(s) 2342
Bme1390I CCNGG 5 cut(s) 858, 1693, 2018, 2019, 2542
Bme18I GGWCC 5 cut(s) 690, 729, 1570, 1649, 1880
BmeT110I CYCGRG 5 cut(s) 1428, 2017, 2147, 2527, 2690
BmgT120I GGNCC 5 cut(s) 690, 729, 1570, 1649, 1880
BmiI GGNNCC 7 cut(s) 535, 691, 730, 768, 1168, 1882, 2539
BmrFI CCNGG 5 cut(s) 858, 1693, 2018, 2019, 2542
BmrI ACTGGG 1 cut(s) 357
BmsI GCATC 4 cut(s) 385, 1102, 2124, 2586
BmtI GCTAGC 2 cut(s) 204, 749
BmuI ACTGGG 1 cut(s) 357
BpiI GAAGAC 4 cut(s) 787, 875, 956, 2116
BpmI CTGGAG 2 cut(s) 518, 2426
Bpu10I CCTNAGC 2 cut(s) 1289, 1487
BpuEI CTTGAG 4 cut(s) 353, 614, 1730, 2420
BpuMI CCSGG 2 cut(s) 2018, 2019
BsaAI YACGTR 1 cut(s) 1556
BsaHI GRCGYC 1 cut(s) 2068
BsaI GGTCTC 1 cut(s) 1751
BsaJI CCNNGG 9 cut(s) 143, 360, 375, 988, 1257, 1338, 2016, 2017, 2184
BsaXI ACNNNNNCTCC 2 cut(s) 746, 776
Bsc4I CCNNNNNNNGG 6 cut(s) 776, 814, 863, 1750, 1956, 2534
Bse118I RCCGGY 2 cut(s) 974, 1132
BseBI CCWGG 3 cut(s) 858, 1693, 2542
BseDI CCNNGG 9 cut(s) 143, 360, 375, 988, 1257, 1338, 2016, 2017, 2184
BseGI GGATG 5 cut(s) 1267, 1792, 2113, 2158, 2644
BseLI CCNNNNNNNGG 6 cut(s) 776, 814, 863, 1750, 1956, 2534
BseMII CTCAG 4 cut(s) 1478, 1893, 2028, 2580
BseRI GAGGAG 4 cut(s) 456, 699, 767, 1733
BseSI GKGCMC 1 cut(s) 1074
BseXI GCAGC 8 cut(s) 343, 593, 1102, 1106, 1336, 1909, 2688, 2691
BsgI GTGCAG 1 cut(s) 1335
BshFI GGCC 2 cut(s) 2253, 2545
BsiHKAI GWGCWC 5 cut(s) 76, 659, 1074, 1908, 2670
BsiHKCI CYCGRG 5 cut(s) 1428, 2017, 2147, 2527, 2690
BsiSI CCGG 3 cut(s) 975, 1133, 2018
BslFI GGGAC 4 cut(s) 358, 703, 742, 1866
BslI CCNNNNNNNGG 6 cut(s) 776, 814, 863, 1750, 1956, 2534
BsmAI GTCTC 3 cut(s) 144, 1751, 2560
BsmFI GGGAC 4 cut(s) 358, 703, 742, 1866
BsmI GAATGC 1 cut(s) 43
BsnI GGCC 2 cut(s) 2253, 2545
Bso31I GGTCTC 1 cut(s) 1751
BsoBI CYCGRG 5 cut(s) 1428, 2017, 2147, 2527, 2690
Bsp1286I GDGCHC 6 cut(s) 76, 659, 1074, 1828, 1908, 2670
Bsp143I GATC 9 cut(s) 29, 533, 766, 1207, 1378, 1494, 1584, 2238, 2610
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 6 cut(s) 93, 1297, 1321, 1544, 1738, 2682
BspANI GGCC 2 cut(s) 2253, 2545
BspCNI CTCAG 4 cut(s) 1479, 1894, 2029, 2581
BspHI TCATGA 1 cut(s) 2562
BspLI GGNNCC 7 cut(s) 535, 691, 730, 768, 1168, 1882, 2539
BspMAI CTGCAG 1 cut(s) 1158
BspMI ACCTGC 1 cut(s) 85
BspOI GCTAGC 2 cut(s) 204, 749
BspPI GGATC 6 cut(s) 37, 528, 541, 761, 774, 1202
BspQI GCTCTTC 1 cut(s) 2548
BspTNI GGTCTC 1 cut(s) 1751
BsrBI CCGCTC 2 cut(s) 93, 1297
BsrFI RCCGGY 2 cut(s) 974, 1132
BssAI RCCGGY 2 cut(s) 974, 1132
BssECI CCNNGG 9 cut(s) 143, 360, 375, 988, 1257, 1338, 2016, 2017, 2184
BssMI GATC 9 cut(s) 29, 533, 766, 1207, 1378, 1494, 1584, 2238, 2610
BssNI GRCGYC 1 cut(s) 2068
BssSI CACGAG 4 cut(s) 399, 468, 585, 1655
BssT1I CCWWGG 3 cut(s) 360, 375, 1338
Bst2BI CACGAG 4 cut(s) 399, 468, 585, 1655
Bst2UI CCWGG 3 cut(s) 858, 1693, 2542
Bst4CI ACNGT 8 cut(s) 52, 989, 1258, 1993, 2083, 2185, 2340, 2665
Bst6I CTCTTC 2 cut(s) 1749, 2548
BstACI GRCGYC 1 cut(s) 2068
BstAPI GCANNNNNTGC 1 cut(s) 1112
BstBAI YACGTR 1 cut(s) 1556
BstC8I GCNNGC 6 cut(s) 202, 267, 508, 747, 1908, 2601
BstDEI CTNAG 8 cut(s) 741, 828, 1289, 1487, 1902, 2037, 2549, 2589
BstDSI CCRYGG 4 cut(s) 375, 988, 1257, 2184
BstF5I GGATG 5 cut(s) 1267, 1792, 2113, 2158, 2644
BstH2I RGCGCY 1 cut(s) 235
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 9 cut(s) 32, 536, 769, 1210, 1381, 1497, 1587, 2241, 2613
BstMAI GTCTC 3 cut(s) 144, 1751, 2560
BstMBI GATC 9 cut(s) 29, 533, 766, 1207, 1378, 1494, 1584, 2238, 2610
BstMWI GCNNNNNNNGC 7 cut(s) 671, 1094, 1103, 1112, 1294, 1799, 1922
BstNI CCWGG 3 cut(s) 858, 1693, 2542
BstSCI CCNGG 5 cut(s) 856, 1691, 2016, 2017, 2540
BstSFI CTRYAG 2 cut(s) 1154, 2082
BstSLI GKGCMC 1 cut(s) 1074
BstV1I GCAGC 8 cut(s) 343, 593, 1102, 1106, 1336, 1909, 2688, 2691
BstV2I GAAGAC 4 cut(s) 787, 875, 956, 2116
BstX2I RGATCY 4 cut(s) 29, 533, 766, 1207
BstYI RGATCY 4 cut(s) 29, 533, 766, 1207
BsuI GTATCC 1 cut(s) 2513
BsuRI GGCC 2 cut(s) 2253, 2545
BtgI CCRYGG 4 cut(s) 375, 988, 1257, 2184
BtsCI GGATG 5 cut(s) 1267, 1792, 2113, 2158, 2644
BtsI GCAGTG 2 cut(s) 1163, 2239
BveI ACCTGC 1 cut(s) 85
Cac8I GCNNGC 6 cut(s) 202, 267, 508, 747, 1908, 2601
CaiI CAGNNNCTG 1 cut(s) 665
CciI TCATGA 1 cut(s) 2562
CfoI GCGC 1 cut(s) 234
Cfr10I RCCGGY 2 cut(s) 974, 1132
Cfr13I GGNCC 5 cut(s) 690, 729, 1570, 1649, 1880
Cfr9I CCCGGG 1 cut(s) 2017
CseI GACGC 2 cut(s) 2076, 2265
CsiI ACCWGGT 1 cut(s) 856
Csp6I GTAC 6 cut(s) 63, 978, 985, 1186, 1369, 2341
CspCI CAANNNNNGTGG 2 cut(s) 1873, 1908
CviAII CATG 9 cut(s) 55, 223, 376, 1445, 1951, 1978, 2131, 2563, 2584
CviQI GTAC 6 cut(s) 63, 978, 985, 1186, 1369, 2341
DdeI CTNAG 8 cut(s) 741, 828, 1289, 1487, 1902, 2037, 2549, 2589
DpnI GATC 9 cut(s) 31, 535, 768, 1209, 1380, 1496, 1586, 2240, 2612
DpnII GATC 9 cut(s) 29, 533, 766, 1207, 1378, 1494, 1584, 2238, 2610
Eam1104I CTCTTC 2 cut(s) 1749, 2548
EarI CTCTTC 2 cut(s) 1749, 2548
Ecl136II GAGCTC 1 cut(s) 1906
Eco130I CCWWGG 3 cut(s) 360, 375, 1338
Eco147I AGGCCT 1 cut(s) 2545
Eco24I GRGCYC 2 cut(s) 1828, 1908
Eco31I GGTCTC 1 cut(s) 1751
Eco47I GGWCC 5 cut(s) 690, 729, 1570, 1649, 1880
Eco47III AGCGCT 1 cut(s) 233
Eco53kI GAGCTC 1 cut(s) 1906
Eco57I CTGAAG 5 cut(s) 1479, 1683, 1962, 2076, 2329
Eco88I CYCGRG 5 cut(s) 1428, 2017, 2147, 2527, 2690
EcoICRI GAGCTC 1 cut(s) 1906
EcoO109I RGGNCCY 1 cut(s) 1649
EcoRI GAATTC 1 cut(s) 1701
EcoRII CCWGG 3 cut(s) 856, 1691, 2540
EcoT14I CCWWGG 3 cut(s) 360, 375, 1338
EcoT38I GRGCYC 2 cut(s) 1828, 1908
ErhI CCWWGG 3 cut(s) 360, 375, 1338
FaeI CATG 9 cut(s) 58, 226, 379, 1448, 1954, 1981, 2134, 2566, 2587
FaqI GGGAC 4 cut(s) 358, 703, 742, 1866
FatI CATG 9 cut(s) 54, 222, 375, 1444, 1950, 1977, 2130, 2562, 2583
FauI CCCGC 1 cut(s) 1537
Fnu4HI GCNGC 8 cut(s) 357, 582, 1095, 1116, 1350, 1923, 2677, 2680
FokI GGATG 5 cut(s) 1274, 1799, 2100, 2165, 2651
FriOI GRGCYC 2 cut(s) 1828, 1908
Fsp4HI GCNGC 8 cut(s) 357, 582, 1095, 1116, 1350, 1923, 2677, 2680
FspBI CTAG 6 cut(s) 42, 201, 290, 615, 716, 746
GlaI GCGC 1 cut(s) 233
GluI GCNGC 8 cut(s) 357, 582, 1095, 1116, 1350, 1923, 2677, 2680
GsuI CTGGAG 2 cut(s) 518, 2426
HaeII RGCGCY 1 cut(s) 235
HaeIII GGCC 2 cut(s) 2253, 2545
HapII CCGG 3 cut(s) 975, 1133, 2018
HgaI GACGC 2 cut(s) 2076, 2265
HhaI GCGC 1 cut(s) 234
Hin1I GRCGYC 1 cut(s) 2068
Hin1II CATG 9 cut(s) 58, 226, 379, 1448, 1954, 1981, 2134, 2566, 2587
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HincII GTYRAC 2 cut(s) 1722, 2512
HindII GTYRAC 2 cut(s) 1722, 2512
HindIII AAGCTT 1 cut(s) 1914
HpaII CCGG 3 cut(s) 975, 1133, 2018
Hpy166II GTNNAC 6 cut(s) 63, 194, 1072, 1151, 1722, 2512
Hpy8I GTNNAC 6 cut(s) 63, 194, 1072, 1151, 1722, 2512
Hpy99I CGWCG 2 cut(s) 2070, 2259
HpyCH4III ACNGT 8 cut(s) 52, 989, 1258, 1993, 2083, 2185, 2340, 2665
HpyCH4IV ACGT 3 cut(s) 799, 1555, 2658
HpyF10VI GCNNNNNNNGC 7 cut(s) 671, 1094, 1103, 1112, 1294, 1799, 1922
HpyF3I CTNAG 8 cut(s) 741, 828, 1289, 1487, 1902, 2037, 2549, 2589
HpySE526I ACGT 3 cut(s) 799, 1555, 2658
Hsp92I GRCGYC 1 cut(s) 2068
Hsp92II CATG 9 cut(s) 58, 226, 379, 1448, 1954, 1981, 2134, 2566, 2587
HspAI GCGC 1 cut(s) 232
Kzo9I GATC 9 cut(s) 29, 533, 766, 1207, 1378, 1494, 1584, 2238, 2610
LguI GCTCTTC 1 cut(s) 2548
LmnI GCTCC 9 cut(s) 313, 487, 1085, 1091, 1166, 1294, 1323, 2142, 2537
Lsp1109I GCAGC 8 cut(s) 343, 593, 1102, 1106, 1336, 1909, 2688, 2691
LweI GCATC 4 cut(s) 385, 1102, 2124, 2586
MabI ACCWGGT 1 cut(s) 856
MaeI CTAG 6 cut(s) 42, 201, 290, 615, 716, 746
MaeII ACGT 3 cut(s) 799, 1555, 2658
MalI GATC 9 cut(s) 31, 535, 768, 1209, 1380, 1496, 1586, 2240, 2612
MbiI CCGCTC 2 cut(s) 93, 1297
MboI GATC 9 cut(s) 29, 533, 766, 1207, 1378, 1494, 1584, 2238, 2610
MfeI CAATTG 3 cut(s) 876, 1107, 1401
MflI RGATCY 4 cut(s) 29, 533, 766, 1207
MhlI GDGCHC 6 cut(s) 76, 659, 1074, 1828, 1908, 2670
MlyI GAGTC 3 cut(s) 2562, 2618, 2702
MmeI TCCRAC 8 cut(s) 661, 744, 757, 1255, 1813, 2206, 2349, 2413
MroXI GAANNNNTTC 2 cut(s) 1269, 2061
MseI TTAA 8 cut(s) 117, 252, 384, 390, 1079, 1127, 2162, 2310
MslI CAYNNNNRTG 2 cut(s) 2084, 2118
MspA1I CMGCKG 3 cut(s) 1592, 2050, 2682
MspI CCGG 3 cut(s) 975, 1133, 2018
MspR9I CCNGG 5 cut(s) 858, 1693, 2018, 2019, 2542
MunI CAATTG 3 cut(s) 876, 1107, 1401
Mva1269I GAATGC 1 cut(s) 43
MvaI CCWGG 3 cut(s) 858, 1693, 2542
MwoI GCNNNNNNNGC 7 cut(s) 671, 1094, 1103, 1112, 1294, 1799, 1922
NciI CCSGG 2 cut(s) 2018, 2019
NcoI CCATGG 1 cut(s) 375
NdeII GATC 9 cut(s) 29, 533, 766, 1207, 1378, 1494, 1584, 2238, 2610
NheI GCTAGC 2 cut(s) 200, 745
NlaIII CATG 9 cut(s) 58, 226, 379, 1448, 1954, 1981, 2134, 2566, 2587
NlaIV GGNNCC 7 cut(s) 535, 691, 730, 768, 1168, 1882, 2539
NmeAIII GCCGAG 2 cut(s) 168, 1847
NmuCI GTSAC 8 cut(s) 293, 492, 1556, 1657, 2320, 2482, 2621, 2659
OliI CACNNNNGTG 1 cut(s) 2084
PaeR7I CTCGAG 2 cut(s) 1428, 2147
PagI TCATGA 1 cut(s) 2562
PaqCI CACCTGC 1 cut(s) 85
PceI AGGCCT 1 cut(s) 2545
PciSI GCTCTTC 1 cut(s) 2548
PcsI WCGNNNNNNNCGW 3 cut(s) 193, 475, 1825
PctI GAATGC 1 cut(s) 43
PdmI GAANNNNTTC 2 cut(s) 1269, 2061
PfeI GAWTC 7 cut(s) 1227, 1306, 1730, 1843, 1954, 2645, 2710
PflFI GACNNNGTC 3 cut(s) 291, 2070, 2627
PflMI CCANNNNNTGG 1 cut(s) 814
PfoI TCCNGGA 1 cut(s) 1691
PkrI GCNGC 8 cut(s) 358, 583, 1096, 1117, 1351, 1924, 2678, 2681
PleI GAGTC 3 cut(s) 2562, 2618, 2701
PpsI GAGTC 3 cut(s) 2562, 2618, 2701
Ppu21I YACGTR 1 cut(s) 1556
PpuMI RGGWCCY 1 cut(s) 1649
PshBI ATTAAT 1 cut(s) 384
PsiI TTATAA 1 cut(s) 947
Psp124BI GAGCTC 1 cut(s) 1908
Psp5II RGGWCCY 1 cut(s) 1649
Psp6I CCWGG 3 cut(s) 856, 1691, 2540
PspGI CCWGG 3 cut(s) 856, 1691, 2540
PspN4I GGNNCC 7 cut(s) 535, 691, 730, 768, 1168, 1882, 2539
PspPI GGNCC 5 cut(s) 690, 729, 1570, 1649, 1880
PspPPI RGGWCCY 1 cut(s) 1649
PspXI VCTCGAGB 1 cut(s) 2147
PstI CTGCAG 1 cut(s) 1158
PstNI CAGNNNCTG 1 cut(s) 665
PsuI RGATCY 4 cut(s) 29, 533, 766, 1207
PsyI GACNNNGTC 3 cut(s) 291, 2070, 2627
PvuII CAGCTG 2 cut(s) 1592, 2050
RsaI GTAC 6 cut(s) 64, 979, 986, 1187, 1370, 2342
RsaNI GTAC 6 cut(s) 63, 978, 985, 1186, 1369, 2341
RseI CAYNNNNRTG 2 cut(s) 2084, 2118
SacI GAGCTC 1 cut(s) 1908
SapI GCTCTTC 1 cut(s) 2548
SaqAI TTAA 8 cut(s) 117, 252, 384, 390, 1079, 1127, 2162, 2310
SatI GCNGC 8 cut(s) 357, 582, 1095, 1116, 1350, 1923, 2677, 2680
Sau3AI GATC 9 cut(s) 29, 533, 766, 1207, 1378, 1494, 1584, 2238, 2610
Sau96I GGNCC 5 cut(s) 690, 729, 1570, 1649, 1880
ScaI AGTACT 1 cut(s) 2342
SchI GAGTC 3 cut(s) 2562, 2618, 2702
ScrFI CCNGG 5 cut(s) 858, 1693, 2018, 2019, 2542
SduI GDGCHC 6 cut(s) 76, 659, 1074, 1828, 1908, 2670
SexAI ACCWGGT 1 cut(s) 856
SfaNI GCATC 4 cut(s) 385, 1102, 2124, 2586
SfcI CTRYAG 2 cut(s) 1154, 2082
Sfr274I CTCGAG 2 cut(s) 1428, 2147
SinI GGWCC 5 cut(s) 690, 729, 1570, 1649, 1880
SlaI CTCGAG 2 cut(s) 1428, 2147
SmaI CCCGGG 1 cut(s) 2019
SmiMI CAYNNNNRTG 2 cut(s) 2084, 2118
SmlI CTYRAG 6 cut(s) 368, 593, 1428, 1745, 2147, 2435
SmoI CTYRAG 6 cut(s) 368, 593, 1428, 1745, 2147, 2435
SseBI AGGCCT 1 cut(s) 2545
SsiI CCGC 6 cut(s) 93, 1297, 1321, 1544, 1738, 2682
SspMI CTAG 6 cut(s) 42, 201, 290, 615, 716, 746
SstI GAGCTC 1 cut(s) 1908
StuI AGGCCT 1 cut(s) 2545
StyD4I CCNGG 5 cut(s) 856, 1691, 2016, 2017, 2540
StyI CCWWGG 3 cut(s) 360, 375, 1338
TaaI ACNGT 8 cut(s) 52, 989, 1258, 1993, 2083, 2185, 2340, 2665
TaiI ACGT 3 cut(s) 802, 1558, 2661
TaqI TCGA 7 cut(s) 1179, 1429, 2065, 2148, 2241, 2448, 2643
TaqII GACCGA 1 cut(s) 2617
TatI WGTACW 4 cut(s) 62, 1185, 1368, 2340
TfiI GAWTC 7 cut(s) 1227, 1306, 1730, 1843, 1954, 2645, 2710
Tru1I TTAA 8 cut(s) 117, 252, 384, 390, 1079, 1127, 2162, 2310
Tru9I TTAA 8 cut(s) 117, 252, 384, 390, 1079, 1127, 2162, 2310
TseFI GTSAC 8 cut(s) 293, 492, 1556, 1657, 2320, 2482, 2621, 2659
TseI GCWGC 8 cut(s) 356, 581, 1094, 1115, 1349, 1922, 2676, 2679
Tsp45I GTSAC 8 cut(s) 293, 492, 1556, 1657, 2320, 2482, 2621, 2659
TspDTI ATGAA 8 cut(s) 1278, 1347, 1431, 1743, 1803, 1858, 1966, 2112
TspGWI ACGGA 4 cut(s) 326, 913, 2339, 2550
TspMI CCCGGG 1 cut(s) 2017
Tth111I GACNNNGTC 3 cut(s) 291, 2070, 2627
Van91I CCANNNNNTGG 1 cut(s) 814
VneI GTGCAC 1 cut(s) 1070
VpaK11BI GGWCC 5 cut(s) 690, 729, 1570, 1649, 1880
VspI ATTAAT 1 cut(s) 384
XapI RAATTY 5 cut(s) 170, 453, 598, 810, 1701
XbaI TCTAGA 1 cut(s) 715
XhoI CTCGAG 2 cut(s) 1428, 2147
XmaI CCCGGG 1 cut(s) 2017
XmnI GAANNNNTTC 2 cut(s) 1269, 2061
XspI CTAG 6 cut(s) 42, 201, 290, 615, 716, 746
ZrmI AGTACT 1 cut(s) 2342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.