Rh7AG403400

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0004579
Physical Location & Seq
Forward (+)
53288925 .. 53290046
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG403400.1

Sequence Viewer

Length: 1122 bp
ATGGACCTTAAAACACAGATCAGAAATATAATAGAGAAAGGAAAAGAGATGAGCAAGGCTGAGAGCGAGGCAGGGGATGTAGGTCCTATGGTAACTGAAGTGGATATTCAACGTATCGTGTTCTCTTGGACTGGTATTCCTGTTGACAAAGTGTCAGCTGATGAATCGGATCGCCTTCTCAAGATGGAAGAGACACTCCATAAGCGGGTTATTGGTCAGGATGAAGCAGTCAAGGCTAATAGTCGTGCTATTCGCCGAGGCCGTGTTGGACTCAAATATCCTAATCGTCCAATTGCCAGTTTCATCTTTTCTGGTCCAACTGGTGTTGGAAAATCCGAACTCGCAAAAGCTTTGGCTGCATACTACTTTGGCTCTGAAGAAGCCATGATTTGCCTTGATATGAGTGAATTTATGGAACGGCACTCTGTTTCAAAGCTTATTGGTTCTCCCCCTGGTTATGTTGGTTACACTGATGGAGGTCAGCTGACTGAAGCCGTTAGACGCCGTCCTTACACTGTGGTGCTATTTGATGAGATTGAGAAGGCTGATCCAGATGTCTTAAATGTGATGCTTCAAATTCTTGAGGATGGAAGATTGACCGACAGGAAGGGCAGAACTGTGGATTTCAAGAATACACTTCTGATCATGACATCTAATGTCGGAAGCAGTGTAATTGAGAAAGGAGGACTCCGTGTAGGATTTGATCTTGATTACGATGAGAATGATAGCAGTCACAATCGCATCAAGAGCTTAGTGACAGAGGAGCTAAAACAGCAATTTAGACCTGAGTTCTTAAATAGGTTGGATGAAATCATTGTCTTCCGCCAGCTGACTAAGTTGGAGCCGAAGGAAATAGCAGATATAATGCTGAAGGAGGTTTCCGAAAGGCTGAAGACTAAAGAGATACAGCTTCAAGTGACAGAGAGGTTCAGGGATAGCTTGGTTGATGATGGATACAACCCAAGCTATGGGGCAAGGCCTCTAAGAAGAGCCATAATGAGACTTTTGGAGGACAACATGGCTGAGAAGATGCTAGCAAGGGAAATCAAAGAGGGCGACACAGTTATTGTGAATGTCGATTATGATGGGAATGTAATTGTGCTCAATCAGTGGTTCTCCTGA

Protein Analysis

373

Amino Acids

42.28

Weight (kDa)

5.31

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 96 - 271 1.8e-66 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 101 - 222 4.8e-12 AAA domain (dynein-related subfamily)
AAA PF00004 102 - 220 2.3e-06 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 277 - 357 5e-25 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 968
AciI CCGC 2 cut(s) 205, 823
AclWI GGATC 2 cut(s) 177, 542
AcsI RAATTY 2 cut(s) 407, 576
AcuI CTGAAG 5 cut(s) 117, 396, 510, 890, 911
AcyI GRCGYC 1 cut(s) 502
AfiI CCNNNNNNNGG 2 cut(s) 205, 968
AgsI TTSAA 5 cut(s) 110, 432, 575, 628, 914
AhdI GACNNNNNGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 451
AleI CACNNNNGTG 1 cut(s) 518
Alw21I GWGCWC 1 cut(s) 1104
Alw26I GTCTC 2 cut(s) 185, 994
AlwI GGATC 2 cut(s) 177, 542
AoxI GGCC 2 cut(s) 259, 977
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 2 cut(s) 407, 576
AspS9I GGNCC 3 cut(s) 4, 83, 314
AsuNHI GCTAGC 1 cut(s) 1033
AvaII GGWCC 3 cut(s) 4, 83, 314
BbsI GAAGAC 2 cut(s) 811, 899
Bbv12I GWGCWC 1 cut(s) 1104
BbvI GCAGC 1 cut(s) 343
BccI CCATC 5 cut(s) 178, 467, 581, 944, 1079
BceAI ACGGC 4 cut(s) 246, 434, 479, 489
BciT130I CCWGG 1 cut(s) 453
BciVI GTATCC 1 cut(s) 947
BclI TGATCA 1 cut(s) 642
BcoDI GTCTC 2 cut(s) 185, 994
BfaI CTAG 1 cut(s) 1034
BfuI GTATCC 1 cut(s) 947
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 3 cut(s) 4, 83, 314
BmeRI GACNNNNNGTC 1 cut(s) 151
BmgT120I GGNCC 3 cut(s) 4, 83, 314
BmiI GGNNCC 1 cut(s) 843
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 3 cut(s) 558, 750, 1020
BmtI GCTAGC 1 cut(s) 1037
BpiI GAAGAC 2 cut(s) 811, 899
BpuEI CTTGAG 2 cut(s) 164, 602
BsaHI GRCGYC 1 cut(s) 502
BsaJI CCNNGG 2 cut(s) 256, 451
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 968
Bse1I ACTGG 3 cut(s) 136, 297, 325
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 2 cut(s) 256, 451
BseGI GGATG 4 cut(s) 82, 226, 592, 811
BseLI CCNNNNNNNGG 2 cut(s) 205, 968
BseMII CTCAG 3 cut(s) 51, 777, 1014
BseNI ACTGG 3 cut(s) 136, 297, 325
BseRI GAGGAG 1 cut(s) 776
BseXI GCAGC 1 cut(s) 343
BshFI GGCC 2 cut(s) 261, 979
BsiHKAI GWGCWC 1 cut(s) 1104
BslI CCNNNNNNNGG 2 cut(s) 205, 968
BsmAI GTCTC 2 cut(s) 185, 994
BsnI GGCC 2 cut(s) 261, 979
Bsp1286I GDGCHC 1 cut(s) 1104
Bsp143I GATC 5 cut(s) 18, 169, 547, 642, 703
BspACI CCGC 2 cut(s) 205, 823
BspANI GGCC 2 cut(s) 261, 979
BspCNI CTCAG 3 cut(s) 52, 778, 1015
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 1 cut(s) 843
BspOI GCTAGC 1 cut(s) 1037
BspPI GGATC 2 cut(s) 177, 542
BspQI GCTCTTC 1 cut(s) 982
BsrI ACTGG 3 cut(s) 136, 297, 325
BssECI CCNNGG 2 cut(s) 256, 451
BssMI GATC 5 cut(s) 18, 169, 547, 642, 703
BssNI GRCGYC 1 cut(s) 502
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 3 cut(s) 517, 619, 1063
Bst6I CTCTTC 2 cut(s) 183, 982
BstACI GRCGYC 1 cut(s) 502
BstC8I GCNNGC 2 cut(s) 827, 1035
BstDEI CTNAG 6 cut(s) 60, 751, 786, 834, 983, 1023
BstF5I GGATG 4 cut(s) 82, 226, 592, 811
BstKTI GATC 5 cut(s) 21, 172, 550, 645, 706
BstMAI GTCTC 2 cut(s) 185, 994
BstMBI GATC 5 cut(s) 18, 169, 547, 642, 703
BstMWI GCNNNNNNNGC 4 cut(s) 233, 356, 747, 772
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 2 cut(s) 811, 899
BsuI GTATCC 1 cut(s) 947
BsuRI GGCC 2 cut(s) 261, 979
BtsCI GGATG 4 cut(s) 82, 226, 592, 811
BtsI GCAGTG 1 cut(s) 673
BtsIMutI CAGTG 4 cut(s) 468, 513, 673, 1115
Cac8I GCNNGC 2 cut(s) 827, 1035
CciI TCATGA 1 cut(s) 645
Cfr13I GGNCC 3 cut(s) 4, 83, 314
CseI GACGC 1 cut(s) 510
CviAII CATG 3 cut(s) 385, 646, 1018
DdeI CTNAG 6 cut(s) 60, 751, 786, 834, 983, 1023
DpnI GATC 5 cut(s) 20, 171, 549, 644, 705
DpnII GATC 5 cut(s) 18, 169, 547, 642, 703
DriI GACNNNNNGTC 1 cut(s) 151
Eam1104I CTCTTC 2 cut(s) 183, 982
Eam1105I GACNNNNNGTC 1 cut(s) 151
EarI CTCTTC 2 cut(s) 183, 982
EciI GGCGGA 1 cut(s) 812
Eco147I AGGCCT 1 cut(s) 979
Eco47I GGWCC 3 cut(s) 4, 83, 314
Eco57I CTGAAG 5 cut(s) 117, 396, 510, 890, 911
EcoO109I RGGNCCY 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 451
FaeI CATG 3 cut(s) 388, 649, 1021
FatI CATG 3 cut(s) 384, 645, 1017
FauI CCCGC 1 cut(s) 198
FbaI TGATCA 1 cut(s) 642
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 4 cut(s) 89, 233, 599, 818
Fsp4HI GCNGC 1 cut(s) 357
FspBI CTAG 1 cut(s) 1034
GluI GCNGC 1 cut(s) 357
HaeIII GGCC 2 cut(s) 261, 979
HgaI GACGC 1 cut(s) 510
Hin1I GRCGYC 1 cut(s) 502
Hin1II CATG 3 cut(s) 388, 649, 1021
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 2 cut(s) 348, 434
HinfI GANTC 3 cut(s) 164, 270, 687
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 7 cut(s) 23, 169, 337, 376, 642, 662, 883
Hpy188III TCNNGA 9 cut(s) 181, 218, 551, 581, 628, 646, 707, 745, 1119
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 5 cut(s) 185, 535, 601, 841, 865
HpyCH4III ACNGT 3 cut(s) 517, 619, 1063
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 356, 747, 772
HpyF3I CTNAG 6 cut(s) 60, 751, 786, 834, 983, 1023
HpySE526I ACGT 1 cut(s) 112
Hsp92I GRCGYC 1 cut(s) 502
Hsp92II CATG 3 cut(s) 388, 649, 1021
Ksp22I TGATCA 1 cut(s) 642
Kzo9I GATC 5 cut(s) 18, 169, 547, 642, 703
LguI GCTCTTC 1 cut(s) 982
LmnI GCTCC 2 cut(s) 763, 841
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 3 cut(s) 558, 750, 1020
MaeI CTAG 1 cut(s) 1034
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 5 cut(s) 91, 464, 731, 754, 916
MalI GATC 5 cut(s) 20, 171, 549, 644, 705
MboI GATC 5 cut(s) 18, 169, 547, 642, 703
MboII GAAGA 7 cut(s) 200, 389, 603, 811, 904, 999, 1039
MfeI CAATTG 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 1104
MluCI AATT 6 cut(s) 291, 407, 576, 672, 776, 1095
MlyI GAGTC 2 cut(s) 264, 681
MmeI TCCRAC 6 cut(s) 247, 307, 341, 640, 783, 819
MseI TTAA 3 cut(s) 9, 560, 794
MslI CAYNNNNRTG 1 cut(s) 518
MspA1I CMGCKG 3 cut(s) 158, 484, 829
MspR9I CCNGG 1 cut(s) 453
MunI CAATTG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 453
MwoI GCNNNNNNNGC 4 cut(s) 233, 356, 747, 772
NdeII GATC 5 cut(s) 18, 169, 547, 642, 703
NheI GCTAGC 1 cut(s) 1033
NlaIII CATG 3 cut(s) 388, 649, 1021
NlaIV GGNNCC 1 cut(s) 843
NmeAIII GCCGAG 1 cut(s) 281
NmuCI GTSAC 3 cut(s) 731, 754, 916
OliI CACNNNNGTG 1 cut(s) 518
PagI TCATGA 1 cut(s) 645
PceI AGGCCT 1 cut(s) 979
PciSI GCTCTTC 1 cut(s) 982
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PfeI GAWTC 1 cut(s) 164
PflFI GACNNNGTC 1 cut(s) 504
PflMI CCANNNNNTGG 1 cut(s) 968
PkrI GCNGC 1 cut(s) 358
PleI GAGTC 2 cut(s) 264, 681
PpsI GAGTC 2 cut(s) 264, 681
PpuMI RGGWCCY 1 cut(s) 83
Psp5II RGGWCCY 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 843
PspPI GGNCC 3 cut(s) 4, 83, 314
PspPPI RGGWCCY 1 cut(s) 83
PsyI GACNNNGTC 1 cut(s) 504
PvuII CAGCTG 3 cut(s) 158, 484, 829
RseI CAYNNNNRTG 1 cut(s) 518
SapI GCTCTTC 1 cut(s) 982
SaqAI TTAA 3 cut(s) 9, 560, 794
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 5 cut(s) 18, 169, 547, 642, 703
Sau96I GGNCC 3 cut(s) 4, 83, 314
SchI GAGTC 2 cut(s) 264, 681
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 1 cut(s) 1104
SfaNI GCATC 3 cut(s) 558, 750, 1020
SinI GGWCC 3 cut(s) 4, 83, 314
SmiMI CAYNNNNRTG 1 cut(s) 518
SmlI CTYRAG 2 cut(s) 179, 581
SmoI CTYRAG 2 cut(s) 179, 581
Sse9I AATT 6 cut(s) 291, 407, 576, 672, 776, 1095
SseBI AGGCCT 1 cut(s) 979
SsiI CCGC 2 cut(s) 205, 823
SspMI CTAG 1 cut(s) 1034
StuI AGGCCT 1 cut(s) 979
StyD4I CCNGG 1 cut(s) 451
TaaI ACNGT 3 cut(s) 517, 619, 1063
TaiI ACGT 1 cut(s) 115
TaqI TCGA 1 cut(s) 1077
TaqII GACCGA 1 cut(s) 614
TasI AATT 6 cut(s) 291, 407, 576, 672, 776, 1095
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 3 cut(s) 9, 560, 794
Tru9I TTAA 3 cut(s) 9, 560, 794
TscAI CASTG 4 cut(s) 475, 520, 673, 1115
TseFI GTSAC 3 cut(s) 731, 754, 916
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 3 cut(s) 731, 754, 916
TspDTI ATGAA 4 cut(s) 177, 237, 292, 822
TspGWI ACGGA 1 cut(s) 680
TspRI CASTG 4 cut(s) 475, 520, 673, 1115
Tth111I GACNNNGTC 1 cut(s) 504
Van91I CCANNNNNTGG 1 cut(s) 968
VpaK11BI GGWCC 3 cut(s) 4, 83, 314
XapI RAATTY 2 cut(s) 407, 576
XspI CTAG 1 cut(s) 1034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.