RLG00000004649

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004579
Physical Location & Seq
Reverse (-)
61105654 .. 61110474
4821 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004649

Sequence Viewer

Length: 2646 bp
ATGGCCAGGGTTTTGGTTCAGTCCACGAATATTCCCGGTGTGGTTGCCGGGCGAAGGCTAGGCCAATCTAAAGGATCTGGAAATTCTAGAAGGTCGACCAAGATGATGTGCACCTTACGAGCACCTGCGTTGAGACTGAGCAGTTTCTCAGGACTACGCAGTGTTAATGCTCTAGATATTATGGCGAGGCCTGGGCAGGACTTGTATTCTAAGGTGGGGGTAGCTATCTCCTCCCGGCGTCAAAAGGCTAGCAGATGTGTGCCTAAAGCCATGTTTGAGCGCTTCACAGAAAAAGCAATAAAGGTGATTATGCTTGCACAAGAGGAAGCAAGGCGACTTGGTCACAACTTTGTTGGTACGGAGCAGATACTTTTGGGTCTTATTGGTGAAGGAACTGGAATTGCTGCCAAGGTCCTTAAATCTATGGGAATCAATCTTAAAGATGCACGCGTTGAAGTGGAAAAGATCATTGGAAGGGGTAGTGGCTTTGTTGCTGTTGAAATCCCATTCACTCCTCGTGCAAAGCGAGTTTTGGAACTGTCACTGGAGGAAGCTCGACAACTGGGCCATAACTATATTGGTTCTGAACACTTGCTTTTGGGACTGCTTCGGGAAGGTGAGGGTGTGGCAGCTCGTGTTCTTGAGAATTTAGGCGCTGACCCCAGCAACATTCGCACACAGGGAAAGCTGGATCCTGTTGTTGGAAGACAACCACAAATTGAACGTGTTGTCCAAATTTTGGGCAGGCGAACAAAGAATAATCCTTGCCTGATTGGGGAACCTGGTGTTGGGAAGACTGCAATTGCTGAAGGTCTTGCTCAACGAATTGCAAGCGGTGATGTGCCTGAAACAATAGAGGGAAAGAAGGTTATCACTCTGGATATGGGTCTTCTTGTTGCTGGTACAAAATATCGTGGAGAGTTCGAGGAAAGATTAAAGAAGCTGATGGAGGAAATCAAACAAAGTGATGAGATAATTTTGTTCATTGATGAGGTGCACACTTTAATTGGAGCAGGAGCAGCAGAAGGTGCAATTGATGCTGCCAATATATTAAAACCAGCTCTTGCAAGAGGTGAACTGCAGTGTATTGGAGCCACCACGCTGGATGAATACAGAAAGCACATTGAGAAAGACCCAGCGTTGGAAAGACGTTTTCAACCAGTTAAAGTTCCTGAACCATCTGTGGACGAAACAATACAGATTTTGCGAGGGCTCCGGGAGCGATATGAGATCCACCACAAACTCCGGTATACTGATGAAGCTCTAGTATCTGCTGCCCAGCTATCATACCAGTACATCAGTGATCGCTTTCTACCTGATAAAGCAATTGACTTGATTGATGAAGCTGGTTCTCGAGTTCGCCTTCGTCATGCACAGCTACCTGAAGAAGCTAGAGACCTTGACAAAGAACTGAGACAGATTACCAAGGAGAAGAATGAAGCTGTTCGAAGCCAAGACTTTGAAAAGGCTGGGGAGTTGCGTGACAGAGAAATGGACCTCAAGACGCAGATCACAGCTATAACAGAGAAAGGCAAAGAGATGAGCAAGGCTGAGAGCGAGGCAGGGGATGTAGGACCTATGGTAACTGAAGTGGATATTCAACATATTGTGTCCTCTTGGACTGGTATTCCTGTTGACAAAGTGTCAGCTGATGAATCAGATCGCCTTCTCAAGATGGAAGAGACCCTCCATAAGCGGGTTATTGGTCAGGATGAAGCAGTCAAGGCTATTAGTCGTGCTATTCGCCGAGCCCGTGTTGGACTGAAAAATCCTAATCGTCCAATTGCCAGTTTCATCTTTTCTGGTCCAACTGGTGTTGGAAAATCCGAACTCGCAAAAGCTTTGGCTGCATACTACTTTGGCTCTGAAGAAGCCATGATTCGTCTTGATATGAGTGAATTCATGGAAAGGCACACTGTCTCAAAGCTTATTGGTTCTCCCCCTGGTTATGTTGGTTACACCGAGGGAGGTCAGCTTACTGAAGCTGTTAGACGTCGTCCTTACACTGTGGTGCTGTTTGATGAGATTGAGAAGGCTCATCCGGATGTTTTCAACATGATGCTTCAAATTCTTGAGGATGGAAGATTGACTGACAGCAAGGGCAGAACTGTGGATTTCAAGAATACACTTCTGATCATGACATCAAATGTTGGAAGCAGTGTAATTGAGAAAGGAGGCCGACGTATAGGATTTGACCTTGATTATGATGAGAAGGATAGCAGTTACAATCGCATCAAGAGCTTGGTGACAGAGGAGTTGAAACAGTACTTTAGACCAGAGTTCCTAAATAGGTTGGATGAAATGATTGTCTTCCGCCAGCTCACTAAGTTGGAGGTGAAGGAAATAGCAGATATAATGCTGAAAGAGGTTTTCGAAAGGCTGAAGACCAAAGAGATAGAGCTTCAAGTGACAGAGAGGTTCAGAGATAGGGTGGTGGATGAAGGATACAATCCAAGTTATGGGGCAAGGCCTCTAAGAAGAGCCATAATGAGGCTTTTGGAGGACAGCATGGCTGAGAAGATGCTAGCAAGGGAGATTAAAGAGGGCGACTCAGTTATTGTGGATGTTGATTCTGATGGGAACGTAACCGTGCTCAATGGTAGCAGTGGTTCTCCCGAATCTTTGCCAGAGGCTCTTCCCGTTTAA

Protein Analysis

882

Amino Acids

98.06

Weight (kDa)

6.93

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 94 - 216 1.2e-36 Clp repeat (R) N-terminal domain
AAA PF00004 256 - 368 6.2e-14 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 394 - 495 6.6e-35 AAA lid domain
UVR PF02151 468 - 503 3.7e-09 UvrB/uvrC motif
AAA_2 PF07724 593 - 767 9.1e-69 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 598 - 719 1.1e-11 AAA domain (dynein-related subfamily)
AAA PF00004 599 - 718 3.1e-07 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 774 - 854 1.8e-24 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 133
AatII GACGTC 1 cut(s) 1996
Acc36I ACCTGC 1 cut(s) 133
AccB7I CCANNNNNTGG 2 cut(s) 739, 2459
AccI GTMKAC 2 cut(s) 95, 1250
AccII CGCG 1 cut(s) 450
AccIII TCCGGA 1 cut(s) 2041
AciI CCGC 3 cut(s) 834, 1696, 2314
AclWI GGATC 4 cut(s) 82, 686, 699, 1225
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 5 cut(s) 82, 646, 735, 1898, 2067
AcuI CTGAAG 6 cut(s) 828, 1404, 1608, 1887, 2001, 2402
AcyI GRCGYC 2 cut(s) 238, 1993
AfaI GTAC 4 cut(s) 358, 904, 1295, 2267
AfeI AGCGCT 1 cut(s) 281
AfiI CCNNNNNNNGG 9 cut(s) 54, 701, 739, 775, 788, 1141, 1696, 2459, 2490
AflIII ACRYGT 2 cut(s) 448, 724
AhdI GACNNNNNGTC 1 cut(s) 1642
AjnI CCWGG 4 cut(s) 5, 190, 781, 1942
AjuI GAANNNNNNNTTGG 4 cut(s) 453, 485, 771, 803
AleI CACNNNNGTG 1 cut(s) 2009
AloI GAACNNNNNNTCC 2 cut(s) 714, 746
Alw21I GWGCWC 4 cut(s) 113, 124, 999, 2595
Alw26I GTCTC 5 cut(s) 127, 1389, 1408, 1676, 1924
Alw44I GTGCAC 2 cut(s) 109, 995
AlwI GGATC 4 cut(s) 82, 686, 699, 1225
Ama87I CYCGRG 1 cut(s) 1353
Aor13HI TCCGGA 1 cut(s) 2041
Aor51HI AGCGCT 1 cut(s) 281
AoxI GGCC 6 cut(s) 3, 61, 188, 565, 2176, 2468
ApaLI GTGCAC 2 cut(s) 109, 995
ApeKI GCWGC 6 cut(s) 404, 629, 1019, 1040, 1274, 1847
ApoI RAATTY 5 cut(s) 82, 646, 735, 1898, 2067
Asp700I GAANNNNTTC 1 cut(s) 1443
AspLEI GCGC 2 cut(s) 282, 656
AspS9I GGNCC 5 cut(s) 412, 565, 1495, 1574, 1805
AsuC2I CCSGG 4 cut(s) 36, 49, 235, 1217
AsuHPI GGTGA 7 cut(s) 316, 398, 629, 848, 1085, 2257, 2347
AsuII TTCGAA 2 cut(s) 1447, 2373
AsuNHI GCTAGC 2 cut(s) 248, 2524
AvaI CYCGRG 1 cut(s) 1353
AvaII GGWCC 4 cut(s) 412, 1495, 1574, 1805
BaeGI GKGCMC 2 cut(s) 113, 999
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 691
BanII GRGCYC 2 cut(s) 1215, 1753
BauI CACGAG 2 cut(s) 516, 633
BbsI GAAGAC 5 cut(s) 712, 800, 881, 2302, 2390
Bbv12I GWGCWC 4 cut(s) 113, 124, 999, 2595
BbvI GCAGC 6 cut(s) 391, 641, 1027, 1031, 1261, 1834
BccI CCATC 5 cut(s) 940, 1186, 1669, 2072, 2570
BciT130I CCWGG 4 cut(s) 7, 192, 783, 1944
BciVI GTATCC 1 cut(s) 2438
BclI TGATCA 1 cut(s) 2133
BcnI CCSGG 4 cut(s) 36, 49, 235, 1217
BcoDI GTCTC 5 cut(s) 127, 1389, 1408, 1676, 1924
BfaI CTAG 7 cut(s) 59, 87, 173, 249, 1265, 1392, 2525
BfmI CTRYAG 1 cut(s) 1079
BfoI RGCGCY 2 cut(s) 283, 657
BfuAI ACCTGC 1 cut(s) 133
BfuI GTATCC 1 cut(s) 2438
BisI GCNGC 6 cut(s) 405, 630, 1020, 1041, 1275, 1848
BlsI GCNGC 6 cut(s) 406, 631, 1021, 1042, 1276, 1849
BmcAI AGTACT 1 cut(s) 2267
Bme1390I CCNGG 8 cut(s) 7, 36, 49, 192, 235, 783, 1217, 1944
Bme18I GGWCC 4 cut(s) 412, 1495, 1574, 1805
BmeRI GACNNNNNGTC 1 cut(s) 1642
BmeT110I CYCGRG 1 cut(s) 1353
BmgT120I GGNCC 5 cut(s) 412, 565, 1495, 1574, 1805
BmiI GGNNCC 4 cut(s) 693, 780, 1093, 1214
BmrFI CCNGG 8 cut(s) 7, 36, 49, 192, 235, 783, 1217, 1944
BmrI ACTGGG 1 cut(s) 572
BmsI GCATC 5 cut(s) 433, 1027, 2049, 2241, 2511
BmtI GCTAGC 2 cut(s) 252, 2528
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 5 cut(s) 712, 800, 881, 2302, 2390
BplI GAGNNNNNCTC 2 cut(s) 2534, 2566
BpmI CTGGAG 1 cut(s) 566
Bpu14I TTCGAA 2 cut(s) 1447, 2373
BpuEI CTTGAG 4 cut(s) 662, 1484, 1655, 2093
BpuMI CCSGG 4 cut(s) 36, 49, 235, 1217
BsaHI GRCGYC 2 cut(s) 238, 1993
BsaI GGTCTC 2 cut(s) 1389, 1676
BsaJI CCNNGG 6 cut(s) 6, 191, 408, 1425, 1942, 1962
BsaWI WCCGGW 2 cut(s) 1245, 2041
Bsc4I CCNNNNNNNGG 9 cut(s) 54, 701, 739, 775, 788, 1141, 1696, 2459, 2490
Bse1I ACTGG 8 cut(s) 400, 549, 567, 1160, 1291, 1627, 1788, 1816
BseAI TCCGGA 1 cut(s) 2041
BseBI CCWGG 4 cut(s) 7, 192, 783, 1944
BseDI CCNNGG 6 cut(s) 6, 191, 408, 1425, 1942, 1962
BseGI GGATG 9 cut(s) 1111, 1573, 1717, 2038, 2050, 2083, 2302, 2443, 2569
BseLI CCNNNNNNNGG 9 cut(s) 54, 701, 739, 775, 788, 1141, 1696, 2459, 2490
BseMII CTCAG 6 cut(s) 128, 162, 1403, 1542, 2505, 2565
BseNI ACTGG 8 cut(s) 400, 549, 567, 1160, 1291, 1627, 1788, 1816
BseRI GAGGAG 3 cut(s) 220, 504, 2267
BseSI GKGCMC 2 cut(s) 113, 999
BseXI GCAGC 6 cut(s) 391, 641, 1027, 1031, 1261, 1834
BseYI CCCAGC 4 cut(s) 662, 1135, 1278, 1469
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 6 cut(s) 5, 63, 190, 567, 2178, 2470
BsiHKAI GWGCWC 4 cut(s) 113, 124, 999, 2595
BsiHKCI CYCGRG 1 cut(s) 1353
BsiSI CCGG 6 cut(s) 36, 48, 235, 1216, 1246, 2042
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 9 cut(s) 54, 701, 739, 775, 788, 1141, 1696, 2459, 2490
BsmAI GTCTC 5 cut(s) 127, 1389, 1408, 1676, 1924
BsmFI GGGAC 1 cut(s) 615
BsnI GGCC 6 cut(s) 5, 63, 190, 567, 2178, 2470
Bso31I GGTCTC 2 cut(s) 1389, 1676
BsoBI CYCGRG 1 cut(s) 1353
Bsp119I TTCGAA 2 cut(s) 1447, 2373
Bsp1286I GDGCHC 6 cut(s) 113, 124, 999, 1215, 1753, 2595
Bsp13I TCCGGA 1 cut(s) 2041
Bsp143I GATC 8 cut(s) 74, 465, 691, 1230, 1303, 1509, 1660, 2133
BspACI CCGC 3 cut(s) 834, 1696, 2314
BspANI GGCC 6 cut(s) 5, 63, 190, 567, 2178, 2470
BspCNI CTCAG 6 cut(s) 129, 161, 1404, 1543, 2506, 2564
BspEI TCCGGA 1 cut(s) 2041
BspFNI CGCG 1 cut(s) 450
BspHI TCATGA 1 cut(s) 2136
BspLI GGNNCC 4 cut(s) 693, 780, 1093, 1214
BspMAI CTGCAG 1 cut(s) 1083
BspMI ACCTGC 1 cut(s) 133
BspOI GCTAGC 2 cut(s) 252, 2528
BspPI GGATC 4 cut(s) 82, 686, 699, 1225
BspQI GCTCTTC 2 cut(s) 2473, 2640
BspT104I TTCGAA 2 cut(s) 1447, 2373
BspTNI GGTCTC 2 cut(s) 1389, 1676
BsrI ACTGG 8 cut(s) 400, 549, 567, 1160, 1291, 1627, 1788, 1816
BssECI CCNNGG 6 cut(s) 6, 191, 408, 1425, 1942, 1962
BssMI GATC 8 cut(s) 74, 465, 691, 1230, 1303, 1509, 1660, 2133
BssNAI GTATAC 1 cut(s) 1251
BssNI GRCGYC 2 cut(s) 238, 1993
BssSI CACGAG 2 cut(s) 516, 633
BssT1I CCWWGG 2 cut(s) 408, 1425
Bst1107I GTATAC 1 cut(s) 1251
Bst2BI CACGAG 2 cut(s) 516, 633
Bst2UI CCWGG 4 cut(s) 7, 192, 783, 1944
Bst4CI ACNGT 6 cut(s) 540, 1918, 2008, 2110, 2265, 2590
Bst6I CTCTTC 3 cut(s) 1674, 2473, 2640
BstACI GRCGYC 2 cut(s) 238, 1993
BstAPI GCANNNNNTGC 2 cut(s) 1028, 1037
BstBI TTCGAA 2 cut(s) 1447, 2373
BstC8I GCNNGC 7 cut(s) 250, 315, 448, 746, 832, 2318, 2526
BstDEI CTNAG 9 cut(s) 137, 148, 210, 1412, 1551, 2325, 2474, 2514, 2551
BstF5I GGATG 9 cut(s) 1111, 1573, 1717, 2038, 2050, 2083, 2302, 2443, 2569
BstFNI CGCG 1 cut(s) 450
BstH2I RGCGCY 2 cut(s) 283, 657
BstHHI GCGC 2 cut(s) 282, 656
BstKTI GATC 8 cut(s) 77, 468, 694, 1233, 1306, 1512, 1663, 2136
BstMAI GTCTC 5 cut(s) 127, 1389, 1408, 1676, 1924
BstMBI GATC 8 cut(s) 74, 465, 691, 1230, 1303, 1509, 1660, 2133
BstMWI GCNNNNNNNGC 8 cut(s) 672, 1019, 1028, 1037, 1219, 1724, 1847, 2238
BstNI CCWGG 4 cut(s) 7, 192, 783, 1944
BstSCI CCNGG 8 cut(s) 5, 34, 47, 190, 233, 781, 1215, 1942
BstSFI CTRYAG 1 cut(s) 1079
BstSLI GKGCMC 2 cut(s) 113, 999
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 6 cut(s) 391, 641, 1027, 1031, 1261, 1834
BstV2I GAAGAC 5 cut(s) 712, 800, 881, 2302, 2390
BstX2I RGATCY 3 cut(s) 74, 691, 1230
BstXI CCANNNNNNTGG 2 cut(s) 13, 1102
BstYI RGATCY 3 cut(s) 74, 691, 1230
BstZ17I GTATAC 1 cut(s) 1251
BsuI GTATCC 1 cut(s) 2438
BsuRI GGCC 6 cut(s) 5, 63, 190, 567, 2178, 2470
BtsCI GGATG 9 cut(s) 1111, 1573, 1717, 2038, 2050, 2083, 2302, 2443, 2569
BtsI GCAGTG 4 cut(s) 166, 1088, 2164, 2611
BtsIMutI CAGTG 8 cut(s) 166, 542, 1088, 1306, 1914, 2004, 2164, 2611
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 7 cut(s) 250, 315, 448, 746, 832, 2318, 2526
CciI TCATGA 1 cut(s) 2136
CfoI GCGC 2 cut(s) 282, 656
Cfr13I GGNCC 5 cut(s) 412, 565, 1495, 1574, 1805
CseI GACGC 2 cut(s) 227, 1513
CsiI ACCWGGT 1 cut(s) 781
Csp6I GTAC 4 cut(s) 357, 903, 1294, 2266
CviAII CATG 7 cut(s) 271, 1370, 1876, 1903, 2056, 2137, 2509
CviQI GTAC 4 cut(s) 357, 903, 1294, 2266
DdeI CTNAG 9 cut(s) 137, 148, 210, 1412, 1551, 2325, 2474, 2514, 2551
DpnI GATC 8 cut(s) 76, 467, 693, 1232, 1305, 1511, 1662, 2135
DpnII GATC 8 cut(s) 74, 465, 691, 1230, 1303, 1509, 1660, 2133
DriI GACNNNNNGTC 1 cut(s) 1642
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 1674, 2473, 2640
Eam1105I GACNNNNNGTC 1 cut(s) 1642
EarI CTCTTC 3 cut(s) 1674, 2473, 2640
EciI GGCGGA 1 cut(s) 2303
Eco130I CCWWGG 2 cut(s) 408, 1425
Eco147I AGGCCT 2 cut(s) 190, 2470
Eco24I GRGCYC 2 cut(s) 1215, 1753
Eco31I GGTCTC 2 cut(s) 1389, 1676
Eco47I GGWCC 4 cut(s) 412, 1495, 1574, 1805
Eco47III AGCGCT 1 cut(s) 281
Eco57I CTGAAG 6 cut(s) 828, 1404, 1608, 1887, 2001, 2402
Eco88I CYCGRG 1 cut(s) 1353
EcoO109I RGGNCCY 2 cut(s) 412, 1574
EcoRI GAATTC 1 cut(s) 1898
EcoRII CCWGG 4 cut(s) 5, 190, 781, 1942
EcoT14I CCWWGG 2 cut(s) 408, 1425
EcoT38I GRGCYC 2 cut(s) 1215, 1753
ErhI CCWWGG 2 cut(s) 408, 1425
FaeI CATG 7 cut(s) 274, 1373, 1879, 1906, 2059, 2140, 2512
FaqI GGGAC 1 cut(s) 615
FatI CATG 7 cut(s) 270, 1369, 1875, 1902, 2055, 2136, 2508
FauI CCCGC 1 cut(s) 1689
FbaI TGATCA 1 cut(s) 2133
FblI GTMKAC 2 cut(s) 95, 1250
Fnu4HI GCNGC 6 cut(s) 405, 630, 1020, 1041, 1275, 1848
FokI GGATG 9 cut(s) 1118, 1580, 1724, 2025, 2057, 2090, 2309, 2450, 2576
FriOI GRGCYC 2 cut(s) 1215, 1753
Fsp4HI GCNGC 6 cut(s) 405, 630, 1020, 1041, 1275, 1848
FspBI CTAG 7 cut(s) 59, 87, 173, 249, 1265, 1392, 2525
GlaI GCGC 2 cut(s) 281, 655
GluI GCNGC 6 cut(s) 405, 630, 1020, 1041, 1275, 1848
GsaI CCCAGC 4 cut(s) 666, 1139, 1282, 1473
GsuI CTGGAG 1 cut(s) 566
HaeII RGCGCY 2 cut(s) 283, 657
HaeIII GGCC 6 cut(s) 5, 63, 190, 567, 2178, 2470
HapII CCGG 6 cut(s) 36, 48, 235, 1216, 1246, 2042
HgaI GACGC 2 cut(s) 227, 1513
HhaI GCGC 2 cut(s) 282, 656
Hin1I GRCGYC 2 cut(s) 238, 1993
Hin1II CATG 7 cut(s) 274, 1373, 1879, 1906, 2059, 2140, 2512
Hin6I GCGC 2 cut(s) 280, 654
HinP1I GCGC 2 cut(s) 280, 654
HincII GTYRAC 2 cut(s) 96, 1636
HindII GTYRAC 2 cut(s) 96, 1636
HindIII AAGCTT 2 cut(s) 1839, 1925
HinfI GANTC 6 cut(s) 429, 1655, 1879, 2549, 2570, 2618
HpaII CCGG 6 cut(s) 36, 48, 235, 1216, 1246, 2042
HphI GGTGA 7 cut(s) 316, 398, 629, 848, 1085, 2257, 2347
Hpy166II GTNNAC 8 cut(s) 24, 96, 111, 997, 1076, 1186, 1251, 1636
Hpy188I TCNGA 7 cut(s) 586, 1660, 1828, 1867, 2133, 2423, 2575
Hpy8I GTNNAC 8 cut(s) 24, 96, 111, 997, 1076, 1186, 1251, 1636
Hpy99I CGWCG 2 cut(s) 1998, 2184
HpyCH4III ACNGT 6 cut(s) 540, 1918, 2008, 2110, 2265, 2590
HpyCH4IV ACGT 5 cut(s) 724, 1150, 1993, 2182, 2583
HpyF10VI GCNNNNNNNGC 8 cut(s) 672, 1019, 1028, 1037, 1219, 1724, 1847, 2238
HpyF3I CTNAG 9 cut(s) 137, 148, 210, 1412, 1551, 2325, 2474, 2514, 2551
HpySE526I ACGT 5 cut(s) 724, 1150, 1993, 2182, 2583
Hsp92I GRCGYC 2 cut(s) 238, 1993
Hsp92II CATG 7 cut(s) 274, 1373, 1879, 1906, 2059, 2140, 2512
HspAI GCGC 2 cut(s) 280, 654
Kpn2I TCCGGA 1 cut(s) 2041
Ksp22I TGATCA 1 cut(s) 2133
Kzo9I GATC 8 cut(s) 74, 465, 691, 1230, 1303, 1509, 1660, 2133
LguI GCTCTTC 2 cut(s) 2473, 2640
LmnI GCTCC 6 cut(s) 361, 1010, 1016, 1091, 1218, 1219
Lsp1109I GCAGC 6 cut(s) 391, 641, 1027, 1031, 1261, 1834
LweI GCATC 5 cut(s) 433, 1027, 2049, 2241, 2511
MabI ACCWGGT 1 cut(s) 781
MaeI CTAG 7 cut(s) 59, 87, 173, 249, 1265, 1392, 2525
MaeII ACGT 5 cut(s) 724, 1150, 1993, 2182, 2583
MaeIII GTNAC 9 cut(s) 341, 540, 1481, 1582, 1955, 2222, 2245, 2407, 2584
MalI GATC 8 cut(s) 76, 467, 693, 1232, 1305, 1511, 1662, 2135
MboI GATC 8 cut(s) 74, 465, 691, 1230, 1303, 1509, 1660, 2133
MfeI CAATTG 4 cut(s) 801, 1032, 1326, 1782
MflI RGATCY 3 cut(s) 74, 691, 1230
MhlI GDGCHC 6 cut(s) 113, 124, 999, 1215, 1753, 2595
MlsI TGGCCA 1 cut(s) 5
MluI ACGCGT 1 cut(s) 448
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 2543
MmeI TCCRAC 8 cut(s) 682, 1122, 1738, 1798, 1832, 2131, 2274, 2310
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 2041
MroXI GAANNNNTTC 1 cut(s) 1443
MscI TGGCCA 1 cut(s) 5
MseI TTAA 9 cut(s) 165, 417, 438, 935, 1004, 1052, 1164, 2538, 2644
MslI CAYNNNNRTG 2 cut(s) 2009, 2043
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 1649
MspI CCGG 6 cut(s) 36, 48, 235, 1216, 1246, 2042
MspR9I CCNGG 8 cut(s) 7, 36, 49, 192, 235, 783, 1217, 1944
MunI CAATTG 4 cut(s) 801, 1032, 1326, 1782
MvaI CCWGG 4 cut(s) 7, 192, 783, 1944
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 8 cut(s) 672, 1019, 1028, 1037, 1219, 1724, 1847, 2238
NciI CCSGG 4 cut(s) 36, 49, 235, 1217
NdeII GATC 8 cut(s) 74, 465, 691, 1230, 1303, 1509, 1660, 2133
NheI GCTAGC 2 cut(s) 248, 2524
NlaIII CATG 7 cut(s) 274, 1373, 1879, 1906, 2059, 2140, 2512
NlaIV GGNNCC 4 cut(s) 693, 780, 1093, 1214
NmeAIII GCCGAG 1 cut(s) 1772
NmuCI GTSAC 5 cut(s) 341, 540, 1481, 2245, 2407
NspV TTCGAA 2 cut(s) 1447, 2373
OliI CACNNNNGTG 1 cut(s) 2009
PaeR7I CTCGAG 1 cut(s) 1353
PagI TCATGA 1 cut(s) 2136
PaqCI CACCTGC 1 cut(s) 133
PceI AGGCCT 2 cut(s) 190, 2470
PciSI GCTCTTC 2 cut(s) 2473, 2640
PcsI WCGNNNNNNNCGW 2 cut(s) 523, 1750
PdmI GAANNNNTTC 1 cut(s) 1443
PfeI GAWTC 5 cut(s) 429, 1655, 1879, 2570, 2618
PflFI GACNNNGTC 2 cut(s) 339, 1995
PflMI CCANNNNNTGG 2 cut(s) 739, 2459
PfoI TCCNGGA 1 cut(s) 1215
PkrI GCNGC 6 cut(s) 406, 631, 1021, 1042, 1276, 1849
PleI GAGTC 1 cut(s) 2543
PpsI GAGTC 1 cut(s) 2543
PpuMI RGGWCCY 2 cut(s) 412, 1574
Psp5II RGGWCCY 2 cut(s) 412, 1574
Psp6I CCWGG 4 cut(s) 5, 190, 781, 1942
PspFI CCCAGC 4 cut(s) 662, 1135, 1278, 1469
PspGI CCWGG 4 cut(s) 5, 190, 781, 1942
PspN4I GGNNCC 4 cut(s) 693, 780, 1093, 1214
PspPI GGNCC 5 cut(s) 412, 565, 1495, 1574, 1805
PspPPI RGGWCCY 2 cut(s) 412, 1574
PsrI GAACNNNNNNTAC 2 cut(s) 2593, 2625
PstI CTGCAG 1 cut(s) 1083
PsuI RGATCY 3 cut(s) 74, 691, 1230
PsyI GACNNNGTC 2 cut(s) 339, 1995
PvuII CAGCTG 1 cut(s) 1649
RsaI GTAC 4 cut(s) 358, 904, 1295, 2267
RsaNI GTAC 4 cut(s) 357, 903, 1294, 2266
RseI CAYNNNNRTG 2 cut(s) 2009, 2043
SalI GTCGAC 1 cut(s) 94
SapI GCTCTTC 2 cut(s) 2473, 2640
SaqAI TTAA 9 cut(s) 165, 417, 438, 935, 1004, 1052, 1164, 2538, 2644
SatI GCNGC 6 cut(s) 405, 630, 1020, 1041, 1275, 1848
Sau3AI GATC 8 cut(s) 74, 465, 691, 1230, 1303, 1509, 1660, 2133
Sau96I GGNCC 5 cut(s) 412, 565, 1495, 1574, 1805
ScaI AGTACT 1 cut(s) 2267
SchI GAGTC 1 cut(s) 2543
ScrFI CCNGG 8 cut(s) 7, 36, 49, 192, 235, 783, 1217, 1944
SduI GDGCHC 6 cut(s) 113, 124, 999, 1215, 1753, 2595
SexAI ACCWGGT 1 cut(s) 781
SfaNI GCATC 5 cut(s) 433, 1027, 2049, 2241, 2511
SfcI CTRYAG 1 cut(s) 1079
Sfr274I CTCGAG 1 cut(s) 1353
SfuI TTCGAA 2 cut(s) 1447, 2373
SinI GGWCC 4 cut(s) 412, 1495, 1574, 1805
SlaI CTCGAG 1 cut(s) 1353
SmiMI CAYNNNNRTG 2 cut(s) 2009, 2043
SmlI CTYRAG 5 cut(s) 641, 1353, 1499, 1670, 2072
SmoI CTYRAG 5 cut(s) 641, 1353, 1499, 1670, 2072
SseBI AGGCCT 2 cut(s) 190, 2470
SsiI CCGC 3 cut(s) 834, 1696, 2314
SspI AATATT 1 cut(s) 31
SspMI CTAG 7 cut(s) 59, 87, 173, 249, 1265, 1392, 2525
StuI AGGCCT 2 cut(s) 190, 2470
StyD4I CCNGG 8 cut(s) 5, 34, 47, 190, 233, 781, 1215, 1942
StyI CCWWGG 2 cut(s) 408, 1425
TaaI ACNGT 6 cut(s) 540, 1918, 2008, 2110, 2265, 2590
TaiI ACGT 5 cut(s) 727, 1153, 1996, 2185, 2586
TaqI TCGA 6 cut(s) 95, 556, 924, 1354, 1447, 2373
TatI WGTACW 2 cut(s) 1293, 2265
TfiI GAWTC 5 cut(s) 429, 1655, 1879, 2570, 2618
Tru1I TTAA 9 cut(s) 165, 417, 438, 935, 1004, 1052, 1164, 2538, 2644
Tru9I TTAA 9 cut(s) 165, 417, 438, 935, 1004, 1052, 1164, 2538, 2644
TscAI CASTG 8 cut(s) 166, 549, 1088, 1306, 1921, 2011, 2164, 2611
TseFI GTSAC 5 cut(s) 341, 540, 1481, 2245, 2407
TseI GCWGC 6 cut(s) 404, 629, 1019, 1040, 1274, 1847
Tsp45I GTSAC 5 cut(s) 341, 540, 1481, 2245, 2407
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 8 cut(s) 166, 549, 1088, 1306, 1921, 2011, 2164, 2611
Tth111I GACNNNGTC 2 cut(s) 339, 1995
Van91I CCANNNNNTGG 2 cut(s) 739, 2459
VneI GTGCAC 2 cut(s) 109, 995
VpaK11BI GGWCC 4 cut(s) 412, 1495, 1574, 1805
XapI RAATTY 5 cut(s) 82, 646, 735, 1898, 2067
XbaI TCTAGA 2 cut(s) 86, 172
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XhoI CTCGAG 1 cut(s) 1353
XmiI GTMKAC 2 cut(s) 95, 1250
XmnI GAANNNNTTC 1 cut(s) 1443
XspI CTAG 7 cut(s) 59, 87, 173, 249, 1265, 1392, 2525
ZraI GACGTC 1 cut(s) 1994
ZrmI AGTACT 1 cut(s) 2267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.