RchiOBHm_Chr7g0188931

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004579
Physical Location & Seq
Forward (+)
8304878 .. 8310214
5337 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16874

Sequence Viewer

Length: 2724 bp
ATGGCTAGGGTTTTGGTTCAGTCCACGAATATTCCCGGTGTGGTTGGCGGGCGAAGGCTTGCCCAATCTAAAGGATCTGGAAATACTAGAAGGTCGACCAAGATGATGTGTACCTTGCGAGCACCTGCTTTGAGACTGAGCAGTTTCTCCGGACTACGTAGTGTTAATGCTCTAGATATTATGGCGAGGCCTGGGCAAGACTTGTATTCTAAGGTGGGAGTAGCTATCTCCTCCCGGCGTCAAAAGGCTAGTAGATGTGTGCCCAAAGCCATGTTTGAGCGCTTCACAGAAAAAGCAATAAAGGTGATTATGCTTGCACAAGAGGAAGCAAGGCGACTCGGTCACAACTTTGTTGGTACAGAGCAGATACTTTTGGGTCTTATTGGTGAAGGAACTGGAATTGCTGCCAAGGTCCTTAAATCTATGGGAATCAATCTTAAAGATGCACGCGTTGAAGTGGAAAAGATCATTGGAAGGGGTAGTGGCTTTGTTGCTGTTGAAATCCCATTCACTCCTCGTGCAAAGCGAGTTTTGGAACTGTCACTGGAGGAAGCTCGACAACTGGGCCATAACTATATTGGTTCTGAACACTTGCTTTTGGGACTGCTTCGGGAAGGTGAGGGTGTGGCAGCTCGTGTTATTCGTATGGTGGGTGAGAGCACAGAAGCTGTTGGTGTTGGAGCTGGACAGGGAAGCAGTGGCAACAAGATGCCAACTCTGGAGGAGTATGGTACCAATTTGACTAAGCTAGCAGAGGAGGGAAAGCTGGATCCTGTTGTTGGAAGACAACCACAAATTGAACGTGTTGTCCAAATTTTGGGTAGGCGGACGAAGAATAACCCCTGCCTGATTGGGGAACCTGGTGTTGGGAAGACTGCAATTGCTGAAGGTCTTGCTCAACGAATTGCAAGCGGTGATGTGCCTGAAACAATAGAGGGAAAGAAGGTTATCACTCTGGATATGGGTCTTCTTGTTGCTGGTACAAAATACCGTGGAGAGTTCGAGGAAAGGTTAAAGAAGCTGATGGAGGAAATCAAACAAAGTGATGAGATAATTTTGTTCATTGATGAGGTGCACACTTTAATTGGAGCAGGAGCAGCAGAAGGTGCAATTGATGCTGCCAATATATTAAAACCAGCTCTTGCAAGAGGTGAACTGCAGTGTATTGGAGCCACCACTCTAGATGAATACAGAAAGCACATTGAGAAAGACCCAGCATTGGAAAGACGTTTTCAACCAGTTAAAGTTCCTGAACCATCTGTGGACGAAACAATACAGATTTTGCGAGGGCTCCGGGAGCGATATGAGATCCACCACAAACTCCGGTATACTGATGAAGCTCTAGTATCTGCCGCTCAGCTATCATACCAGTACATCAGTGATCGCTTTCTACCTGATAAAGCAATTGACTTGATTGATGAAGCTGGTTCTCGAGTTCGCCTTCGTCATGCACAGCTACCTGAAGAAGCTAGAGACCTTGACAAAGAACTGAGACAGATTACCAAGGAGAAGAATGAAGCTGTTCGAAGCCAAGACTTTGAAAAGGCTGGGGAGTTGCGTGACAGAGAAATGGACCTCAAGACACAGATCACAGCTATAACAGAGAAAGGCAAAGAGATGAGCAAGGCTGAGAGCGAGGCAGGGGATGTAGGACCTATGGTAACTGAAGTGGATATTCAACATATTGTCTCCTCTTGGACTGGTATTCCTGTTGACAAAGTGTCAGCTGATGAATCAGATCGCCTTCTCAAGATGGAAGAGACCCTCCATAAGCGGGTTATTGGTCAGGATGAAGCAGTCAAGGCTATTAGTCGTGCTATTCGTCGAGCCCGTGTTGGACTGAAAAATCCTAATCGTCCAATTGCCAGTTTCATCTTTTCTGGTCCAACTGGTGTTGGAAAATCCGAACTCGCAAAAGCTTTGGCTGCATACTACTTTGGCTCTGAAGAAGCCATGATTCGTCTTGATATGAGTGAATTCATGGAAAGGCACACTGTCTCAAAGCTTATTGGTTCTCCCCCTGGTTATGTTGGTTACACCGAGGGAGGTCAGCTTACTGAAGCTGTTAGACGTCGTCCTTACACTGTGGTGCTTTTTGATGAGATTGAGAAGGCTCATCCAGATGTCTTCAACATGATGCTTCAAATTCTTGAGGATGGAAGATTGACTGACAGCAAGGGCAGAACTGTGGATTTCAAGAATACACTTCTGATCATGACTTCAAATGTTGGAAGCAGTGTAATTGAGAAAGGAGGCCGACGTATAGGATTTGACCTTGATTATGATGAGAAGGATAGCAGTTACAATCGCATCAAGAGCTTGGTGACAGAGGAGTTGAAACAGTACTTTAGACCAGAGTTCCTAAATAGGTTGGATGAAATGATTGTCTTCCGCCAGCTCACTAAGTTGGAGGTGAAGGAAATAGCGGATATAATGCTGAAAGAGGTTTTCGAAAGGCTGAAGACTAAAGAGATAGAGCTTCAAGTGACAGAGAGGTTCAGAGATAGGGTGGTGGATGAAGGATACAATCCAAGTTATGGGGCAAGGCCTCTAAGAAGAGCGATAATGAGGCTTTTGGAGGACAGCATGGCTGAGAAGATGCTAGCAAGGGAGATTAAAGAGGGCGACTCGGTTATTGTTGATGTTGATTCTGATGGAAACGTAACTGTGCTCAATGGTAGCAGTGGTTCTCCCGAATCTTTGCCAGAGGCTCTTCCCGTTTAA

Protein Analysis

907

Amino Acids

100.66

Weight (kDa)

6.57

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 94 - 215 1.2e-35 Clp repeat (R) N-terminal domain
AAA PF00004 282 - 394 6.4e-14 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 420 - 521 6.8e-35 AAA lid domain
UVR PF02151 494 - 529 3.8e-09 UvrB/uvrC motif
AAA_2 PF07724 619 - 793 9.5e-69 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 624 - 745 1.1e-11 AAA domain (dynein-related subfamily)
AAA PF00004 625 - 744 3.2e-07 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 800 - 880 1.9e-24 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 133
AatII GACGTC 1 cut(s) 2074
Acc36I ACCTGC 1 cut(s) 133
Acc65I GGTACC 1 cut(s) 731
AccB1I GGYRCC 1 cut(s) 731
AccB7I CCANNNNNTGG 2 cut(s) 817, 2537
AccBSI CCGCTC 1 cut(s) 1355
AccI GTMKAC 2 cut(s) 95, 1328
AccII CGCG 1 cut(s) 450
AccIII TCCGGA 1 cut(s) 149
AciI CCGC 7 cut(s) 48, 826, 912, 1353, 1774, 2392, 2426
AclWI GGATC 4 cut(s) 82, 764, 777, 1303
AcsI RAATTY 3 cut(s) 813, 1976, 2145
AcuI CTGAAG 6 cut(s) 906, 1482, 1686, 1965, 2079, 2480
AcyI GRCGYC 2 cut(s) 238, 2071
AfaI GTAC 6 cut(s) 112, 358, 733, 982, 1373, 2345
AfeI AGCGCT 1 cut(s) 281
AfiI CCNNNNNNNGG 7 cut(s) 779, 817, 853, 866, 1219, 1774, 2537
AflIII ACRYGT 2 cut(s) 448, 802
AhdI GACNNNNNGTC 1 cut(s) 1720
AjnI CCWGG 3 cut(s) 190, 859, 2020
AjuI GAANNNNNNNTTGG 4 cut(s) 453, 485, 849, 881
AleI CACNNNNGTG 1 cut(s) 2087
AloI GAACNNNNNNTCC 2 cut(s) 792, 824
Alw21I GWGCWC 4 cut(s) 124, 662, 1077, 2673
Alw26I GTCTC 6 cut(s) 127, 1467, 1486, 1693, 1754, 2002
Alw44I GTGCAC 1 cut(s) 1073
AlwI GGATC 4 cut(s) 82, 764, 777, 1303
AlwNI CAGNNNCTG 1 cut(s) 668
Ama87I CYCGRG 1 cut(s) 1431
Aor13HI TCCGGA 1 cut(s) 149
Aor51HI AGCGCT 1 cut(s) 281
AoxI GGCC 4 cut(s) 188, 565, 2254, 2546
ApaLI GTGCAC 1 cut(s) 1073
ApeKI GCWGC 5 cut(s) 404, 629, 1097, 1118, 1925
ApoI RAATTY 3 cut(s) 813, 1976, 2145
Asp700I GAANNNNTTC 1 cut(s) 1521
Asp718I GGTACC 1 cut(s) 731
AspLEI GCGC 1 cut(s) 282
AspS9I GGNCC 5 cut(s) 412, 565, 1573, 1652, 1883
AsuC2I CCSGG 3 cut(s) 36, 235, 1295
AsuHPI GGTGA 8 cut(s) 316, 398, 629, 665, 926, 1163, 2335, 2425
AsuII TTCGAA 2 cut(s) 1525, 2451
AsuNHI GCTAGC 2 cut(s) 748, 2602
AvaI CYCGRG 1 cut(s) 1431
AvaII GGWCC 4 cut(s) 412, 1573, 1652, 1883
BaeGI GKGCMC 2 cut(s) 264, 1077
BamHI GGATCC 1 cut(s) 769
BanI GGYRCC 1 cut(s) 731
BanII GRGCYC 2 cut(s) 1293, 1831
BauI CACGAG 2 cut(s) 516, 633
BbsI GAAGAC 6 cut(s) 790, 878, 959, 2119, 2380, 2468
Bbv12I GWGCWC 4 cut(s) 124, 662, 1077, 2673
BbvI GCAGC 5 cut(s) 391, 641, 1105, 1109, 1912
BccI CCATC 5 cut(s) 1018, 1264, 1747, 2150, 2648
BciT130I CCWGG 3 cut(s) 192, 861, 2022
BciVI GTATCC 1 cut(s) 2516
BclI TGATCA 1 cut(s) 2211
BcnI CCSGG 3 cut(s) 36, 235, 1295
BcoDI GTCTC 6 cut(s) 127, 1467, 1486, 1693, 1754, 2002
BfaI CTAG 9 cut(s) 6, 87, 173, 249, 749, 1181, 1343, 1470, 2603
BfmI CTRYAG 1 cut(s) 1157
BfoI RGCGCY 1 cut(s) 283
BfuAI ACCTGC 1 cut(s) 133
BfuI GTATCC 1 cut(s) 2516
BisI GCNGC 6 cut(s) 405, 630, 1098, 1119, 1353, 1926
BlpI GCTNAGC 1 cut(s) 1356
BlsI GCNGC 6 cut(s) 406, 631, 1099, 1120, 1354, 1927
BmcAI AGTACT 1 cut(s) 2345
Bme1390I CCNGG 6 cut(s) 36, 192, 235, 861, 1295, 2022
Bme18I GGWCC 4 cut(s) 412, 1573, 1652, 1883
BmeRI GACNNNNNGTC 1 cut(s) 1720
BmeT110I CYCGRG 1 cut(s) 1431
BmgT120I GGNCC 5 cut(s) 412, 565, 1573, 1652, 1883
BmiI GGNNCC 5 cut(s) 733, 771, 858, 1171, 1292
BmrFI CCNGG 6 cut(s) 36, 192, 235, 861, 1295, 2022
BmrI ACTGGG 1 cut(s) 572
BmsI GCATC 6 cut(s) 433, 699, 1105, 2127, 2319, 2589
BmtI GCTAGC 2 cut(s) 752, 2606
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 6 cut(s) 790, 878, 959, 2119, 2380, 2468
BplI GAGNNNNNCTC 2 cut(s) 2612, 2644
BpmI CTGGAG 2 cut(s) 566, 740
Bpu1102I GCTNAGC 1 cut(s) 1356
Bpu14I TTCGAA 2 cut(s) 1525, 2451
BpuEI CTTGAG 3 cut(s) 1562, 1733, 2171
BpuMI CCSGG 3 cut(s) 36, 235, 1295
BsaAI YACGTR 1 cut(s) 158
BsaHI GRCGYC 2 cut(s) 238, 2071
BsaI GGTCTC 2 cut(s) 1467, 1754
BsaJI CCNNGG 6 cut(s) 191, 408, 991, 1503, 2020, 2040
BsaWI WCCGGW 2 cut(s) 149, 1323
Bsc4I CCNNNNNNNGG 7 cut(s) 779, 817, 853, 866, 1219, 1774, 2537
Bse1I ACTGG 8 cut(s) 400, 549, 567, 1238, 1369, 1705, 1866, 1894
BseAI TCCGGA 1 cut(s) 149
BseBI CCWGG 3 cut(s) 192, 861, 2022
BseDI CCNNGG 6 cut(s) 191, 408, 991, 1503, 2020, 2040
BseGI GGATG 6 cut(s) 1651, 1795, 2116, 2161, 2380, 2521
BseLI CCNNNNNNNGG 7 cut(s) 779, 817, 853, 866, 1219, 1774, 2537
BseMII CTCAG 5 cut(s) 128, 1370, 1481, 1620, 2583
BseNI ACTGG 8 cut(s) 400, 549, 567, 1238, 1369, 1705, 1866, 1894
BseRI GAGGAG 6 cut(s) 220, 504, 737, 770, 1681, 2345
BseSI GKGCMC 2 cut(s) 264, 1077
BseXI GCAGC 5 cut(s) 391, 641, 1105, 1109, 1912
BseYI CCCAGC 2 cut(s) 1213, 1547
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 4 cut(s) 190, 567, 2256, 2548
BshNI GGYRCC 1 cut(s) 731
BsiHKAI GWGCWC 4 cut(s) 124, 662, 1077, 2673
BsiHKCI CYCGRG 1 cut(s) 1431
BsiSI CCGG 5 cut(s) 36, 150, 235, 1294, 1324
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 7 cut(s) 779, 817, 853, 866, 1219, 1774, 2537
BsmAI GTCTC 6 cut(s) 127, 1467, 1486, 1693, 1754, 2002
BsmFI GGGAC 1 cut(s) 615
BsnI GGCC 4 cut(s) 190, 567, 2256, 2548
Bso31I GGTCTC 2 cut(s) 1467, 1754
BsoBI CYCGRG 1 cut(s) 1431
Bsp119I TTCGAA 2 cut(s) 1525, 2451
Bsp1286I GDGCHC 7 cut(s) 124, 264, 662, 1077, 1293, 1831, 2673
Bsp13I TCCGGA 1 cut(s) 149
Bsp143I GATC 8 cut(s) 74, 465, 769, 1308, 1381, 1587, 1738, 2211
Bsp1720I GCTNAGC 1 cut(s) 1356
BspACI CCGC 7 cut(s) 48, 826, 912, 1353, 1774, 2392, 2426
BspANI GGCC 4 cut(s) 190, 567, 2256, 2548
BspCNI CTCAG 5 cut(s) 129, 1369, 1482, 1621, 2584
BspEI TCCGGA 1 cut(s) 149
BspFNI CGCG 1 cut(s) 450
BspHI TCATGA 1 cut(s) 2214
BspLI GGNNCC 5 cut(s) 733, 771, 858, 1171, 1292
BspMAI CTGCAG 1 cut(s) 1161
BspMI ACCTGC 1 cut(s) 133
BspOI GCTAGC 2 cut(s) 752, 2606
BspPI GGATC 4 cut(s) 82, 764, 777, 1303
BspQI GCTCTTC 2 cut(s) 2551, 2718
BspT104I TTCGAA 2 cut(s) 1525, 2451
BspT107I GGYRCC 1 cut(s) 731
BspTNI GGTCTC 2 cut(s) 1467, 1754
BsrBI CCGCTC 1 cut(s) 1355
BsrI ACTGG 8 cut(s) 400, 549, 567, 1238, 1369, 1705, 1866, 1894
BssECI CCNNGG 6 cut(s) 191, 408, 991, 1503, 2020, 2040
BssMI GATC 8 cut(s) 74, 465, 769, 1308, 1381, 1587, 1738, 2211
BssNAI GTATAC 1 cut(s) 1329
BssNI GRCGYC 2 cut(s) 238, 2071
BssSI CACGAG 2 cut(s) 516, 633
BssT1I CCWWGG 2 cut(s) 408, 1503
Bst1107I GTATAC 1 cut(s) 1329
Bst2BI CACGAG 2 cut(s) 516, 633
Bst2UI CCWGG 3 cut(s) 192, 861, 2022
Bst4CI ACNGT 7 cut(s) 540, 992, 1996, 2086, 2188, 2343, 2668
Bst6I CTCTTC 3 cut(s) 1752, 2551, 2718
BstACI GRCGYC 2 cut(s) 238, 2071
BstAPI GCANNNNNTGC 2 cut(s) 1106, 1115
BstBAI YACGTR 1 cut(s) 158
BstBI TTCGAA 2 cut(s) 1525, 2451
BstC8I GCNNGC 9 cut(s) 50, 60, 120, 315, 448, 750, 910, 2396, 2604
BstDEI CTNAG 9 cut(s) 137, 210, 744, 1356, 1490, 1629, 2403, 2552, 2592
BstDSI CCRYGG 1 cut(s) 991
BstF5I GGATG 6 cut(s) 1651, 1795, 2116, 2161, 2380, 2521
BstFNI CGCG 1 cut(s) 450
BstH2I RGCGCY 1 cut(s) 283
BstHHI GCGC 1 cut(s) 282
BstKTI GATC 8 cut(s) 77, 468, 772, 1311, 1384, 1590, 1741, 2214
BstMAI GTCTC 6 cut(s) 127, 1467, 1486, 1693, 1754, 2002
BstMBI GATC 8 cut(s) 74, 465, 769, 1308, 1381, 1587, 1738, 2211
BstMWI GCNNNNNNNGC 7 cut(s) 1097, 1106, 1115, 1297, 1802, 1925, 2316
BstNI CCWGG 3 cut(s) 192, 861, 2022
BstSCI CCNGG 6 cut(s) 34, 190, 233, 859, 1293, 2020
BstSFI CTRYAG 1 cut(s) 1157
BstSLI GKGCMC 2 cut(s) 264, 1077
BstSNI TACGTA 1 cut(s) 158
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 5 cut(s) 391, 641, 1105, 1109, 1912
BstV2I GAAGAC 6 cut(s) 790, 878, 959, 2119, 2380, 2468
BstX2I RGATCY 3 cut(s) 74, 769, 1308
BstYI RGATCY 3 cut(s) 74, 769, 1308
BstZ17I GTATAC 1 cut(s) 1329
BsuI GTATCC 1 cut(s) 2516
BsuRI GGCC 4 cut(s) 190, 567, 2256, 2548
BtgI CCRYGG 1 cut(s) 991
BtsCI GGATG 6 cut(s) 1651, 1795, 2116, 2161, 2380, 2521
BtsI GCAGTG 4 cut(s) 703, 1166, 2242, 2689
BtsIMutI CAGTG 8 cut(s) 542, 703, 1166, 1384, 1992, 2082, 2242, 2689
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 9 cut(s) 50, 60, 120, 315, 448, 750, 910, 2396, 2604
CaiI CAGNNNCTG 1 cut(s) 668
CciI TCATGA 1 cut(s) 2214
CfoI GCGC 1 cut(s) 282
Cfr13I GGNCC 5 cut(s) 412, 565, 1573, 1652, 1883
CseI GACGC 1 cut(s) 227
CsiI ACCWGGT 1 cut(s) 859
Csp6I GTAC 6 cut(s) 111, 357, 732, 981, 1372, 2344
CviAII CATG 7 cut(s) 271, 1448, 1954, 1981, 2134, 2215, 2587
CviQI GTAC 6 cut(s) 111, 357, 732, 981, 1372, 2344
DdeI CTNAG 9 cut(s) 137, 210, 744, 1356, 1490, 1629, 2403, 2552, 2592
DpnI GATC 8 cut(s) 76, 467, 771, 1310, 1383, 1589, 1740, 2213
DpnII GATC 8 cut(s) 74, 465, 769, 1308, 1381, 1587, 1738, 2211
DriI GACNNNNNGTC 1 cut(s) 1720
Eam1104I CTCTTC 3 cut(s) 1752, 2551, 2718
Eam1105I GACNNNNNGTC 1 cut(s) 1720
EarI CTCTTC 3 cut(s) 1752, 2551, 2718
EciI GGCGGA 2 cut(s) 841, 2381
Eco105I TACGTA 1 cut(s) 158
Eco130I CCWWGG 2 cut(s) 408, 1503
Eco147I AGGCCT 2 cut(s) 190, 2548
Eco24I GRGCYC 2 cut(s) 1293, 1831
Eco31I GGTCTC 2 cut(s) 1467, 1754
Eco47I GGWCC 4 cut(s) 412, 1573, 1652, 1883
Eco47III AGCGCT 1 cut(s) 281
Eco57I CTGAAG 6 cut(s) 906, 1482, 1686, 1965, 2079, 2480
Eco88I CYCGRG 1 cut(s) 1431
EcoO109I RGGNCCY 2 cut(s) 412, 1652
EcoRI GAATTC 1 cut(s) 1976
EcoRII CCWGG 3 cut(s) 190, 859, 2020
EcoT14I CCWWGG 2 cut(s) 408, 1503
EcoT38I GRGCYC 2 cut(s) 1293, 1831
ErhI CCWWGG 2 cut(s) 408, 1503
FaeI CATG 7 cut(s) 274, 1451, 1957, 1984, 2137, 2218, 2590
FaqI GGGAC 1 cut(s) 615
FatI CATG 7 cut(s) 270, 1447, 1953, 1980, 2133, 2214, 2586
FauI CCCGC 2 cut(s) 41, 1767
FbaI TGATCA 1 cut(s) 2211
FblI GTMKAC 2 cut(s) 95, 1328
Fnu4HI GCNGC 6 cut(s) 405, 630, 1098, 1119, 1353, 1926
FokI GGATG 6 cut(s) 1658, 1802, 2103, 2168, 2387, 2528
FriOI GRGCYC 2 cut(s) 1293, 1831
Fsp4HI GCNGC 6 cut(s) 405, 630, 1098, 1119, 1353, 1926
FspBI CTAG 9 cut(s) 6, 87, 173, 249, 749, 1181, 1343, 1470, 2603
GlaI GCGC 1 cut(s) 281
GluI GCNGC 6 cut(s) 405, 630, 1098, 1119, 1353, 1926
GsaI CCCAGC 2 cut(s) 1217, 1551
GsuI CTGGAG 2 cut(s) 566, 740
HaeII RGCGCY 1 cut(s) 283
HaeIII GGCC 4 cut(s) 190, 567, 2256, 2548
HapII CCGG 5 cut(s) 36, 150, 235, 1294, 1324
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 282
Hin1I GRCGYC 2 cut(s) 238, 2071
Hin1II CATG 7 cut(s) 274, 1451, 1957, 1984, 2137, 2218, 2590
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HincII GTYRAC 2 cut(s) 96, 1714
HindII GTYRAC 2 cut(s) 96, 1714
HindIII AAGCTT 2 cut(s) 1917, 2003
HinfI GANTC 7 cut(s) 336, 429, 1733, 1957, 2627, 2648, 2696
HpaII CCGG 5 cut(s) 36, 150, 235, 1294, 1324
HphI GGTGA 8 cut(s) 316, 398, 629, 665, 926, 1163, 2335, 2425
Hpy166II GTNNAC 8 cut(s) 24, 96, 111, 1075, 1154, 1264, 1329, 1714
Hpy188I TCNGA 7 cut(s) 586, 1738, 1906, 1945, 2211, 2501, 2653
Hpy8I GTNNAC 8 cut(s) 24, 96, 111, 1075, 1154, 1264, 1329, 1714
Hpy99I CGWCG 3 cut(s) 1827, 2076, 2262
HpyCH4III ACNGT 7 cut(s) 540, 992, 1996, 2086, 2188, 2343, 2668
HpyCH4IV ACGT 6 cut(s) 157, 802, 1228, 2071, 2260, 2661
HpyF10VI GCNNNNNNNGC 7 cut(s) 1097, 1106, 1115, 1297, 1802, 1925, 2316
HpyF3I CTNAG 9 cut(s) 137, 210, 744, 1356, 1490, 1629, 2403, 2552, 2592
HpySE526I ACGT 6 cut(s) 157, 802, 1228, 2071, 2260, 2661
Hsp92I GRCGYC 2 cut(s) 238, 2071
Hsp92II CATG 7 cut(s) 274, 1451, 1957, 1984, 2137, 2218, 2590
HspAI GCGC 1 cut(s) 280
Kpn2I TCCGGA 1 cut(s) 149
KpnI GGTACC 1 cut(s) 735
Ksp22I TGATCA 1 cut(s) 2211
Kzo9I GATC 8 cut(s) 74, 465, 769, 1308, 1381, 1587, 1738, 2211
LguI GCTCTTC 2 cut(s) 2551, 2718
LmnI GCTCC 6 cut(s) 680, 1088, 1094, 1169, 1296, 1297
Lsp1109I GCAGC 5 cut(s) 391, 641, 1105, 1109, 1912
LweI GCATC 6 cut(s) 433, 699, 1105, 2127, 2319, 2589
MabI ACCWGGT 1 cut(s) 859
MaeI CTAG 9 cut(s) 6, 87, 173, 249, 749, 1181, 1343, 1470, 2603
MaeII ACGT 6 cut(s) 157, 802, 1228, 2071, 2260, 2661
MaeIII GTNAC 9 cut(s) 341, 540, 1559, 1660, 2033, 2300, 2323, 2485, 2662
MalI GATC 8 cut(s) 76, 467, 771, 1310, 1383, 1589, 1740, 2213
MbiI CCGCTC 1 cut(s) 1355
MboI GATC 8 cut(s) 74, 465, 769, 1308, 1381, 1587, 1738, 2211
MfeI CAATTG 4 cut(s) 879, 1110, 1404, 1860
MflI RGATCY 3 cut(s) 74, 769, 1308
MhlI GDGCHC 7 cut(s) 124, 264, 662, 1077, 1293, 1831, 2673
MluI ACGCGT 1 cut(s) 448
MlyI GAGTC 2 cut(s) 330, 2621
MmeI TCCRAC 8 cut(s) 658, 760, 1816, 1876, 1910, 2209, 2352, 2388
MroI TCCGGA 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 1521
MseI TTAA 9 cut(s) 165, 417, 438, 1013, 1082, 1130, 1242, 2616, 2722
MslI CAYNNNNRTG 2 cut(s) 2087, 2121
MspA1I CMGCKG 1 cut(s) 1727
MspI CCGG 5 cut(s) 36, 150, 235, 1294, 1324
MspR9I CCNGG 6 cut(s) 36, 192, 235, 861, 1295, 2022
MunI CAATTG 4 cut(s) 879, 1110, 1404, 1860
MvaI CCWGG 3 cut(s) 192, 861, 2022
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 7 cut(s) 1097, 1106, 1115, 1297, 1802, 1925, 2316
NciI CCSGG 3 cut(s) 36, 235, 1295
NdeII GATC 8 cut(s) 74, 465, 769, 1308, 1381, 1587, 1738, 2211
NheI GCTAGC 2 cut(s) 748, 2602
NlaIII CATG 7 cut(s) 274, 1451, 1957, 1984, 2137, 2218, 2590
NlaIV GGNNCC 5 cut(s) 733, 771, 858, 1171, 1292
NmuCI GTSAC 5 cut(s) 341, 540, 1559, 2323, 2485
NspV TTCGAA 2 cut(s) 1525, 2451
OliI CACNNNNGTG 1 cut(s) 2087
PaeR7I CTCGAG 1 cut(s) 1431
PagI TCATGA 1 cut(s) 2214
PaqCI CACCTGC 1 cut(s) 133
PceI AGGCCT 2 cut(s) 190, 2548
PciSI GCTCTTC 2 cut(s) 2551, 2718
PcsI WCGNNNNNNNCGW 4 cut(s) 523, 640, 1819, 1828
PdmI GAANNNNTTC 1 cut(s) 1521
PfeI GAWTC 5 cut(s) 429, 1733, 1957, 2648, 2696
PflFI GACNNNGTC 2 cut(s) 339, 2073
PflMI CCANNNNNTGG 2 cut(s) 817, 2537
PfoI TCCNGGA 1 cut(s) 1293
PkrI GCNGC 6 cut(s) 406, 631, 1099, 1120, 1354, 1927
PleI GAGTC 2 cut(s) 330, 2621
PpsI GAGTC 2 cut(s) 330, 2621
Ppu21I YACGTR 1 cut(s) 158
PpuMI RGGWCCY 2 cut(s) 412, 1652
Psp5II RGGWCCY 2 cut(s) 412, 1652
Psp6I CCWGG 3 cut(s) 190, 859, 2020
PspFI CCCAGC 2 cut(s) 1213, 1547
PspGI CCWGG 3 cut(s) 190, 859, 2020
PspN4I GGNNCC 5 cut(s) 733, 771, 858, 1171, 1292
PspPI GGNCC 5 cut(s) 412, 565, 1573, 1652, 1883
PspPPI RGGWCCY 2 cut(s) 412, 1652
PsrI GAACNNNNNNTAC 2 cut(s) 2671, 2703
PstI CTGCAG 1 cut(s) 1161
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 3 cut(s) 74, 769, 1308
PsyI GACNNNGTC 2 cut(s) 339, 2073
PvuII CAGCTG 1 cut(s) 1727
RsaI GTAC 6 cut(s) 112, 358, 733, 982, 1373, 2345
RsaNI GTAC 6 cut(s) 111, 357, 732, 981, 1372, 2344
RseI CAYNNNNRTG 2 cut(s) 2087, 2121
SalI GTCGAC 1 cut(s) 94
SapI GCTCTTC 2 cut(s) 2551, 2718
SaqAI TTAA 9 cut(s) 165, 417, 438, 1013, 1082, 1130, 1242, 2616, 2722
SatI GCNGC 6 cut(s) 405, 630, 1098, 1119, 1353, 1926
Sau3AI GATC 8 cut(s) 74, 465, 769, 1308, 1381, 1587, 1738, 2211
Sau96I GGNCC 5 cut(s) 412, 565, 1573, 1652, 1883
ScaI AGTACT 1 cut(s) 2345
SchI GAGTC 2 cut(s) 330, 2621
ScrFI CCNGG 6 cut(s) 36, 192, 235, 861, 1295, 2022
SduI GDGCHC 7 cut(s) 124, 264, 662, 1077, 1293, 1831, 2673
SexAI ACCWGGT 1 cut(s) 859
SfaNI GCATC 6 cut(s) 433, 699, 1105, 2127, 2319, 2589
SfcI CTRYAG 1 cut(s) 1157
Sfr274I CTCGAG 1 cut(s) 1431
SfuI TTCGAA 2 cut(s) 1525, 2451
SinI GGWCC 4 cut(s) 412, 1573, 1652, 1883
SlaI CTCGAG 1 cut(s) 1431
SmiMI CAYNNNNRTG 2 cut(s) 2087, 2121
SmlI CTYRAG 4 cut(s) 1431, 1577, 1748, 2150
SmoI CTYRAG 4 cut(s) 1431, 1577, 1748, 2150
SnaBI TACGTA 1 cut(s) 158
SseBI AGGCCT 2 cut(s) 190, 2548
SsiI CCGC 7 cut(s) 48, 826, 912, 1353, 1774, 2392, 2426
SspI AATATT 1 cut(s) 31
SspMI CTAG 9 cut(s) 6, 87, 173, 249, 749, 1181, 1343, 1470, 2603
StuI AGGCCT 2 cut(s) 190, 2548
StyD4I CCNGG 6 cut(s) 34, 190, 233, 859, 1293, 2020
StyI CCWWGG 2 cut(s) 408, 1503
TaaI ACNGT 7 cut(s) 540, 992, 1996, 2086, 2188, 2343, 2668
TaiI ACGT 6 cut(s) 160, 805, 1231, 2074, 2263, 2664
TaqI TCGA 7 cut(s) 95, 556, 1002, 1432, 1525, 1825, 2451
TaqII GACCGA 1 cut(s) 329
TatI WGTACW 2 cut(s) 1371, 2343
TauI GCSGC 1 cut(s) 1355
TfiI GAWTC 5 cut(s) 429, 1733, 1957, 2648, 2696
Tru1I TTAA 9 cut(s) 165, 417, 438, 1013, 1082, 1130, 1242, 2616, 2722
Tru9I TTAA 9 cut(s) 165, 417, 438, 1013, 1082, 1130, 1242, 2616, 2722
TscAI CASTG 8 cut(s) 549, 703, 1166, 1384, 1999, 2089, 2242, 2689
TseFI GTSAC 5 cut(s) 341, 540, 1559, 2323, 2485
TseI GCWGC 5 cut(s) 404, 629, 1097, 1118, 1925
Tsp45I GTSAC 5 cut(s) 341, 540, 1559, 2323, 2485
TspRI CASTG 8 cut(s) 549, 703, 1166, 1384, 1999, 2089, 2242, 2689
Tth111I GACNNNGTC 2 cut(s) 339, 2073
Van91I CCANNNNNTGG 2 cut(s) 817, 2537
VneI GTGCAC 1 cut(s) 1073
VpaK11BI GGWCC 4 cut(s) 412, 1573, 1652, 1883
XapI RAATTY 3 cut(s) 813, 1976, 2145
XbaI TCTAGA 2 cut(s) 172, 1180
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XhoI CTCGAG 1 cut(s) 1431
XmiI GTMKAC 2 cut(s) 95, 1328
XmnI GAANNNNTTC 1 cut(s) 1521
XspI CTAG 9 cut(s) 6, 87, 173, 249, 749, 1181, 1343, 1470, 2603
ZraI GACGTC 1 cut(s) 2072
ZrmI AGTACT 1 cut(s) 2345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.