Rw7G008580

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004579
Physical Location & Seq
Forward (+)
7852664 .. 7858106
5443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G008580.1

Sequence Viewer

Length: 2769 bp
ATGGCTAGGGTTTTGGTTCAGTCCACGAATATTCCCGGTGTGGTTGGCGGGCGAAGGCTTGCCCAATCTAAAGGATCTGGAAATACTAGAAGGTCGACCAAGATGATGTGTACCTTACGAGCACCTGCTTTGAGACTGAGCAGTTTCTCCGGACTACGCAGTGTTAATGCTCTAGATATTATGGCGAGGCCTGGGCAAGACTTGTATTCTAAGGTGGGAGTAGCTATCTCCTCCCGGCGTCAAAAGGCTAGCAGATGTGTGCCCAAAGCCATGTTTGAGCGCTTCACAGAAAAAGCAATAAAGGTGATTATGCTTGCACAAGAGGAAGCAAGGCGACTCGGTCACAACTTTGTTGGTACAGAGCAGATACTTTTGGGTCTTATTGGTGAAGGAACTGGAATTGCTGCCAAGGTCCTTAAATCTATGGGAATCAATCTTAAAGATGCACGCGTTGAAGTGGAAAAGATCATTGGAAGGGGTAGTGGCTTTGTTGCTGTTGAAATCCCATTCACTCCTCGTGCAAAGCGAGTTTTGGAACTGTCACTGGAGGAAGCTCGACAACTGGGCCATAACTATATTGGTTCTGAACACTTGCTTTTGGGACTGCTTCGGGAAGGTGAGGGTGTGGCAGCTCGTGTACTTGAGAATTTAGGCGCTGACCCCAGCAACATTCGTACACAGGTTATTCGTATGGTGGGTGAGAGCACAGAAGCTGTTGGTGTTGGAGCTGGACAGGGAAGCAGTGGCAACAAGATGCCAACTCTGGAGGAGTATGGTACCAATTTGACTAAGCTAGCAGAGGAGGGAAAGCTGGATCCTGTTGTTGGAAGACAACCACAAATTGAACGTGTTGTCCAAATTTTGGGTAGGCGGACGAAGAATAACCCCTGCCTGATTGGGGAACCTGGTGTTGGGAAGACTGCAATTGCTGAAGGTCTTGCTCAACGAATTGCAAGCGGTGATGTGCCTGAAACAATAGAGGGAAAGAAGGTTATCACTCTGGATATGGGTCTTCTTGTTGCTGGTACAAAATACCGTGGAGAGTTCGAGGAAAGATTAAAGAAGCTGATGGAGGAAATCAAACAAAGTGATGAGATAATTTTGTTCATTGATGAGGTGCACACTTTAATTGGAGCAGGAGCAGCAGAAGGTGCAATTGATGCTGCCAATATATTAAAACCAGCTCTTGCAAGAGGTGAACTGCAGTGTATTGGAGCCACCACTCTAGATGAATACAGAAAGCACATTGAGAAAGACCCAGCATTGGAAAGACGTTTTCAACCAGTTAAAGTTCCTGAACCATCTGTGGACGAAACAATACAGATTTTGCGAGGGCTCCGGGAGCGATATGAGATCCACCACAAACTCCGGTATACTGATGAAGCTCTAGTATCTGCCGCTCAGCTATCATACCAGTACATCAGTGATCGCTTTCTACCTGATAAAGCAATTGACTTGATTGATGAAGCTGGTTCTCGAGTTCGCCTTCGTCATGCACAGCTACCTGAAGAAGCTAGAGACCTTGACAAAGAACTGAGACAGATTACCAAGGAGAAGAATGAAGCTGTTCGAAGCCAAGACTTTGAAAAGGCTGGGGAGTTGCGTGACAGAGAAATGGACCTCAAGACACAGATCACAGCTATAACAGAGAAAGGCAAAGAGATGAGCAAGGCTGAGAGCGAGGCAGGGGATGTAGGACCTATGGTAACTGAAGTGGATATTCAACATATTGTCTCCTCTTGGACTGGTATTCCTGTTGACAAAGTGTCAGCTGATGAATCAGATCGCCTTCTCAAGATGGAAGAGACCCTCCATAAGCGGGTTATTGGTCAGGATGAAGCAGTCAAGGCTATTAGTCGTGCTATTCGTCGAGCCCGTGTTGGACTGAAAAATCCTAATCGTCCAATTGCCAGTTTCATCTTTTCTGGTCCAACTGGTGTTGGAAAATCCGAACTCGCAAAAGCTTTGGCTGCATACTACTTTGGCTCTGAAGAAGCCATGATTCGTCTTGATATGAGTGAATTCATGGAAAGGCACACTGTCTCAAAGCTTATTGGTTCTCCCCCTGGTTATGTTGGTTACACCGAGGGAGGTCAGCTTACTGAAGCTGTTAGACGTCGTCCTTACACTGTGGTGCTTTTTGATGAGATTGAGAAGGCTCATCCAGATGTCTTCAACATGATGCTTCAAATTCTTGAGGATGGAAGATTGACTGACAGCAAGGGCAGAACTGTGGATTTCAAGAATACACTTCTGATCATGACTTCAAATGTTGGAAGCAGTGTAATTGAGAAAGGAGGCCGACGTATAGGATTTGACCTTGATTATGATGAGAAGGATAGCAGTTACAATCGCATCAAGAGCTTGGTGACAGAGGAGTTGAAACAGTACTTTAGACCAGAGTTCCTAAATAGGTTGGATGAAATGATTGTCTTCCGCCAGCTCACTAAGTTGGAGGTGAAGGAAATAGCGGATATAATGCTGAAAGAGGTTTTCGAAAGGCTGAAGACTAAAGAGATAGAGCTTCAAGTGACAGAGAGGTTCAGAGATAGGGTGGTGGATGAAGGATACAATCCAAGTTATGGGGCAAGGCCTCTAAGAAGAGCGATAATGAGGCTTTTGGAGGACAGCATGGCTGAGAAGATGCTAGCAAGGGAGATTAAAGAGGGCGACTCAGTTATTGTTGATGTTGATTCTGATGGAAACGTAACTGTGCTCAATGGTAGCAGTGGTTCTCCCGAATCTTTGCCAGAGGCTCTTCCCGTTTAA

Protein Analysis

922

Amino Acids

102.27

Weight (kDa)

6.44

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 94 - 216 1.4e-36 Clp repeat (R) N-terminal domain
Clp_N PF02861 170 - 242 8.4e-17 Clp repeat (R) N-terminal domain
AAA PF00004 297 - 409 6.6e-14 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 435 - 536 7e-35 AAA lid domain
UVR PF02151 509 - 544 3.9e-09 UvrB/uvrC motif
AAA_2 PF07724 634 - 808 9.8e-69 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 639 - 760 1.1e-11 AAA domain (dynein-related subfamily)
AAA PF00004 640 - 759 3.3e-07 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 815 - 895 2e-24 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 133
AatII GACGTC 1 cut(s) 2119
Acc36I ACCTGC 1 cut(s) 133
Acc65I GGTACC 1 cut(s) 776
AccB1I GGYRCC 1 cut(s) 776
AccB7I CCANNNNNTGG 2 cut(s) 862, 2582
AccBSI CCGCTC 1 cut(s) 1400
AccI GTMKAC 2 cut(s) 95, 1373
AccII CGCG 1 cut(s) 450
AccIII TCCGGA 1 cut(s) 149
AciI CCGC 7 cut(s) 48, 871, 957, 1398, 1819, 2437, 2471
AclWI GGATC 4 cut(s) 82, 809, 822, 1348
AcsI RAATTY 4 cut(s) 646, 858, 2021, 2190
AcuI CTGAAG 6 cut(s) 951, 1527, 1731, 2010, 2124, 2525
AcyI GRCGYC 2 cut(s) 238, 2116
AfaI GTAC 8 cut(s) 112, 358, 639, 676, 778, 1027, 1418, 2390
AfeI AGCGCT 1 cut(s) 281
AfiI CCNNNNNNNGG 7 cut(s) 824, 862, 898, 911, 1264, 1819, 2582
AflIII ACRYGT 2 cut(s) 448, 847
AhdI GACNNNNNGTC 1 cut(s) 1765
AjnI CCWGG 3 cut(s) 190, 904, 2065
AjuI GAANNNNNNNTTGG 4 cut(s) 453, 485, 894, 926
AleI CACNNNNGTG 1 cut(s) 2132
AloI GAACNNNNNNTCC 2 cut(s) 837, 869
Alw21I GWGCWC 4 cut(s) 124, 707, 1122, 2718
Alw26I GTCTC 6 cut(s) 127, 1512, 1531, 1738, 1799, 2047
Alw44I GTGCAC 1 cut(s) 1118
AlwI GGATC 4 cut(s) 82, 809, 822, 1348
AlwNI CAGNNNCTG 1 cut(s) 713
Ama87I CYCGRG 1 cut(s) 1476
Aor13HI TCCGGA 1 cut(s) 149
Aor51HI AGCGCT 1 cut(s) 281
AoxI GGCC 4 cut(s) 188, 565, 2299, 2591
ApaLI GTGCAC 1 cut(s) 1118
ApeKI GCWGC 5 cut(s) 404, 629, 1142, 1163, 1970
ApoI RAATTY 4 cut(s) 646, 858, 2021, 2190
Asp700I GAANNNNTTC 1 cut(s) 1566
Asp718I GGTACC 1 cut(s) 776
AspLEI GCGC 2 cut(s) 282, 656
AspS9I GGNCC 5 cut(s) 412, 565, 1618, 1697, 1928
AsuC2I CCSGG 3 cut(s) 36, 235, 1340
AsuHPI GGTGA 8 cut(s) 316, 398, 629, 710, 971, 1208, 2380, 2470
AsuII TTCGAA 2 cut(s) 1570, 2496
AsuNHI GCTAGC 3 cut(s) 248, 793, 2647
AvaI CYCGRG 1 cut(s) 1476
AvaII GGWCC 4 cut(s) 412, 1618, 1697, 1928
BaeGI GKGCMC 2 cut(s) 264, 1122
BamHI GGATCC 1 cut(s) 814
BanI GGYRCC 1 cut(s) 776
BanII GRGCYC 2 cut(s) 1338, 1876
BauI CACGAG 2 cut(s) 516, 633
BbsI GAAGAC 6 cut(s) 835, 923, 1004, 2164, 2425, 2513
Bbv12I GWGCWC 4 cut(s) 124, 707, 1122, 2718
BbvI GCAGC 5 cut(s) 391, 641, 1150, 1154, 1957
BccI CCATC 5 cut(s) 1063, 1309, 1792, 2195, 2693
BciT130I CCWGG 3 cut(s) 192, 906, 2067
BciVI GTATCC 1 cut(s) 2561
BclI TGATCA 1 cut(s) 2256
BcnI CCSGG 3 cut(s) 36, 235, 1340
BcoDI GTCTC 6 cut(s) 127, 1512, 1531, 1738, 1799, 2047
BfaI CTAG 9 cut(s) 6, 87, 173, 249, 794, 1226, 1388, 1515, 2648
BfmI CTRYAG 1 cut(s) 1202
BfoI RGCGCY 2 cut(s) 283, 657
BfuAI ACCTGC 1 cut(s) 133
BfuI GTATCC 1 cut(s) 2561
BisI GCNGC 6 cut(s) 405, 630, 1143, 1164, 1398, 1971
BlpI GCTNAGC 1 cut(s) 1401
BlsI GCNGC 6 cut(s) 406, 631, 1144, 1165, 1399, 1972
BmcAI AGTACT 1 cut(s) 2390
Bme1390I CCNGG 6 cut(s) 36, 192, 235, 906, 1340, 2067
Bme18I GGWCC 4 cut(s) 412, 1618, 1697, 1928
BmeRI GACNNNNNGTC 1 cut(s) 1765
BmeT110I CYCGRG 1 cut(s) 1476
BmgT120I GGNCC 5 cut(s) 412, 565, 1618, 1697, 1928
BmiI GGNNCC 5 cut(s) 778, 816, 903, 1216, 1337
BmrFI CCNGG 6 cut(s) 36, 192, 235, 906, 1340, 2067
BmrI ACTGGG 1 cut(s) 572
BmsI GCATC 6 cut(s) 433, 744, 1150, 2172, 2364, 2634
BmtI GCTAGC 3 cut(s) 252, 797, 2651
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 6 cut(s) 835, 923, 1004, 2164, 2425, 2513
BplI GAGNNNNNCTC 2 cut(s) 2657, 2689
BpmI CTGGAG 2 cut(s) 566, 785
Bpu1102I GCTNAGC 1 cut(s) 1401
Bpu14I TTCGAA 2 cut(s) 1570, 2496
BpuEI CTTGAG 4 cut(s) 662, 1607, 1778, 2216
BpuMI CCSGG 3 cut(s) 36, 235, 1340
BsaHI GRCGYC 2 cut(s) 238, 2116
BsaI GGTCTC 2 cut(s) 1512, 1799
BsaJI CCNNGG 6 cut(s) 191, 408, 1036, 1548, 2065, 2085
BsaWI WCCGGW 2 cut(s) 149, 1368
Bsc4I CCNNNNNNNGG 7 cut(s) 824, 862, 898, 911, 1264, 1819, 2582
Bse1I ACTGG 8 cut(s) 400, 549, 567, 1283, 1414, 1750, 1911, 1939
BseAI TCCGGA 1 cut(s) 149
BseBI CCWGG 3 cut(s) 192, 906, 2067
BseDI CCNNGG 6 cut(s) 191, 408, 1036, 1548, 2065, 2085
BseGI GGATG 6 cut(s) 1696, 1840, 2161, 2206, 2425, 2566
BseLI CCNNNNNNNGG 7 cut(s) 824, 862, 898, 911, 1264, 1819, 2582
BseMII CTCAG 6 cut(s) 128, 1415, 1526, 1665, 2628, 2688
BseNI ACTGG 8 cut(s) 400, 549, 567, 1283, 1414, 1750, 1911, 1939
BseRI GAGGAG 6 cut(s) 220, 504, 782, 815, 1726, 2390
BseSI GKGCMC 2 cut(s) 264, 1122
BseXI GCAGC 5 cut(s) 391, 641, 1150, 1154, 1957
BseYI CCCAGC 3 cut(s) 662, 1258, 1592
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 4 cut(s) 190, 567, 2301, 2593
BshNI GGYRCC 1 cut(s) 776
BsiHKAI GWGCWC 4 cut(s) 124, 707, 1122, 2718
BsiHKCI CYCGRG 1 cut(s) 1476
BsiSI CCGG 5 cut(s) 36, 150, 235, 1339, 1369
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 7 cut(s) 824, 862, 898, 911, 1264, 1819, 2582
BsmAI GTCTC 6 cut(s) 127, 1512, 1531, 1738, 1799, 2047
BsmFI GGGAC 1 cut(s) 615
BsnI GGCC 4 cut(s) 190, 567, 2301, 2593
Bso31I GGTCTC 2 cut(s) 1512, 1799
BsoBI CYCGRG 1 cut(s) 1476
Bsp119I TTCGAA 2 cut(s) 1570, 2496
Bsp1286I GDGCHC 7 cut(s) 124, 264, 707, 1122, 1338, 1876, 2718
Bsp13I TCCGGA 1 cut(s) 149
Bsp143I GATC 8 cut(s) 74, 465, 814, 1353, 1426, 1632, 1783, 2256
Bsp1720I GCTNAGC 1 cut(s) 1401
BspACI CCGC 7 cut(s) 48, 871, 957, 1398, 1819, 2437, 2471
BspANI GGCC 4 cut(s) 190, 567, 2301, 2593
BspCNI CTCAG 6 cut(s) 129, 1414, 1527, 1666, 2629, 2687
BspEI TCCGGA 1 cut(s) 149
BspFNI CGCG 1 cut(s) 450
BspHI TCATGA 1 cut(s) 2259
BspLI GGNNCC 5 cut(s) 778, 816, 903, 1216, 1337
BspMAI CTGCAG 1 cut(s) 1206
BspMI ACCTGC 1 cut(s) 133
BspOI GCTAGC 3 cut(s) 252, 797, 2651
BspPI GGATC 4 cut(s) 82, 809, 822, 1348
BspQI GCTCTTC 2 cut(s) 2596, 2763
BspT104I TTCGAA 2 cut(s) 1570, 2496
BspT107I GGYRCC 1 cut(s) 776
BspTNI GGTCTC 2 cut(s) 1512, 1799
BsrBI CCGCTC 1 cut(s) 1400
BsrI ACTGG 8 cut(s) 400, 549, 567, 1283, 1414, 1750, 1911, 1939
BssECI CCNNGG 6 cut(s) 191, 408, 1036, 1548, 2065, 2085
BssMI GATC 8 cut(s) 74, 465, 814, 1353, 1426, 1632, 1783, 2256
BssNAI GTATAC 1 cut(s) 1374
BssNI GRCGYC 2 cut(s) 238, 2116
BssSI CACGAG 2 cut(s) 516, 633
BssT1I CCWWGG 2 cut(s) 408, 1548
Bst1107I GTATAC 1 cut(s) 1374
Bst2BI CACGAG 2 cut(s) 516, 633
Bst2UI CCWGG 3 cut(s) 192, 906, 2067
Bst4CI ACNGT 7 cut(s) 540, 1037, 2041, 2131, 2233, 2388, 2713
Bst6I CTCTTC 3 cut(s) 1797, 2596, 2763
BstACI GRCGYC 2 cut(s) 238, 2116
BstAPI GCANNNNNTGC 2 cut(s) 1151, 1160
BstBI TTCGAA 2 cut(s) 1570, 2496
BstC8I GCNNGC 9 cut(s) 50, 60, 250, 315, 448, 795, 955, 2441, 2649
BstDSI CCRYGG 1 cut(s) 1036
BstF5I GGATG 6 cut(s) 1696, 1840, 2161, 2206, 2425, 2566
BstFNI CGCG 1 cut(s) 450
BstH2I RGCGCY 2 cut(s) 283, 657
BstHHI GCGC 2 cut(s) 282, 656
BstKTI GATC 8 cut(s) 77, 468, 817, 1356, 1429, 1635, 1786, 2259
BstMAI GTCTC 6 cut(s) 127, 1512, 1531, 1738, 1799, 2047
BstMBI GATC 8 cut(s) 74, 465, 814, 1353, 1426, 1632, 1783, 2256
BstMWI GCNNNNNNNGC 7 cut(s) 1142, 1151, 1160, 1342, 1847, 1970, 2361
BstNI CCWGG 3 cut(s) 192, 906, 2067
BstSCI CCNGG 6 cut(s) 34, 190, 233, 904, 1338, 2065
BstSFI CTRYAG 1 cut(s) 1202
BstSLI GKGCMC 2 cut(s) 264, 1122
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 5 cut(s) 391, 641, 1150, 1154, 1957
BstV2I GAAGAC 6 cut(s) 835, 923, 1004, 2164, 2425, 2513
BstX2I RGATCY 3 cut(s) 74, 814, 1353
BstYI RGATCY 3 cut(s) 74, 814, 1353
BstZ17I GTATAC 1 cut(s) 1374
BsuI GTATCC 1 cut(s) 2561
BsuRI GGCC 4 cut(s) 190, 567, 2301, 2593
BtgI CCRYGG 1 cut(s) 1036
BtsCI GGATG 6 cut(s) 1696, 1840, 2161, 2206, 2425, 2566
BtsI GCAGTG 5 cut(s) 166, 748, 1211, 2287, 2734
BtsIMutI CAGTG 9 cut(s) 166, 542, 748, 1211, 1429, 2037, 2127, 2287, 2734
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 9 cut(s) 50, 60, 250, 315, 448, 795, 955, 2441, 2649
CaiI CAGNNNCTG 1 cut(s) 713
CciI TCATGA 1 cut(s) 2259
CfoI GCGC 2 cut(s) 282, 656
Cfr13I GGNCC 5 cut(s) 412, 565, 1618, 1697, 1928
CseI GACGC 1 cut(s) 227
CsiI ACCWGGT 1 cut(s) 904
Csp6I GTAC 8 cut(s) 111, 357, 638, 675, 777, 1026, 1417, 2389
CviAII CATG 7 cut(s) 271, 1493, 1999, 2026, 2179, 2260, 2632
CviQI GTAC 8 cut(s) 111, 357, 638, 675, 777, 1026, 1417, 2389
DpnI GATC 8 cut(s) 76, 467, 816, 1355, 1428, 1634, 1785, 2258
DpnII GATC 8 cut(s) 74, 465, 814, 1353, 1426, 1632, 1783, 2256
DriI GACNNNNNGTC 1 cut(s) 1765
Eam1104I CTCTTC 3 cut(s) 1797, 2596, 2763
Eam1105I GACNNNNNGTC 1 cut(s) 1765
EarI CTCTTC 3 cut(s) 1797, 2596, 2763
EciI GGCGGA 2 cut(s) 886, 2426
Eco130I CCWWGG 2 cut(s) 408, 1548
Eco147I AGGCCT 2 cut(s) 190, 2593
Eco24I GRGCYC 2 cut(s) 1338, 1876
Eco31I GGTCTC 2 cut(s) 1512, 1799
Eco47I GGWCC 4 cut(s) 412, 1618, 1697, 1928
Eco47III AGCGCT 1 cut(s) 281
Eco57I CTGAAG 6 cut(s) 951, 1527, 1731, 2010, 2124, 2525
Eco88I CYCGRG 1 cut(s) 1476
EcoO109I RGGNCCY 2 cut(s) 412, 1697
EcoRI GAATTC 1 cut(s) 2021
EcoRII CCWGG 3 cut(s) 190, 904, 2065
EcoT14I CCWWGG 2 cut(s) 408, 1548
EcoT38I GRGCYC 2 cut(s) 1338, 1876
ErhI CCWWGG 2 cut(s) 408, 1548
FaeI CATG 7 cut(s) 274, 1496, 2002, 2029, 2182, 2263, 2635
FaqI GGGAC 1 cut(s) 615
FatI CATG 7 cut(s) 270, 1492, 1998, 2025, 2178, 2259, 2631
FauI CCCGC 2 cut(s) 41, 1812
FbaI TGATCA 1 cut(s) 2256
FblI GTMKAC 2 cut(s) 95, 1373
Fnu4HI GCNGC 6 cut(s) 405, 630, 1143, 1164, 1398, 1971
FokI GGATG 6 cut(s) 1703, 1847, 2148, 2213, 2432, 2573
FriOI GRGCYC 2 cut(s) 1338, 1876
Fsp4HI GCNGC 6 cut(s) 405, 630, 1143, 1164, 1398, 1971
FspBI CTAG 9 cut(s) 6, 87, 173, 249, 794, 1226, 1388, 1515, 2648
GlaI GCGC 2 cut(s) 281, 655
GluI GCNGC 6 cut(s) 405, 630, 1143, 1164, 1398, 1971
GsaI CCCAGC 3 cut(s) 666, 1262, 1596
GsuI CTGGAG 2 cut(s) 566, 785
HaeII RGCGCY 2 cut(s) 283, 657
HaeIII GGCC 4 cut(s) 190, 567, 2301, 2593
HapII CCGG 5 cut(s) 36, 150, 235, 1339, 1369
HgaI GACGC 1 cut(s) 227
HhaI GCGC 2 cut(s) 282, 656
Hin1I GRCGYC 2 cut(s) 238, 2116
Hin1II CATG 7 cut(s) 274, 1496, 2002, 2029, 2182, 2263, 2635
Hin6I GCGC 2 cut(s) 280, 654
HinP1I GCGC 2 cut(s) 280, 654
HincII GTYRAC 2 cut(s) 96, 1759
HindII GTYRAC 2 cut(s) 96, 1759
HindIII AAGCTT 2 cut(s) 1962, 2048
HinfI GANTC 7 cut(s) 336, 429, 1778, 2002, 2672, 2693, 2741
HpaII CCGG 5 cut(s) 36, 150, 235, 1339, 1369
HphI GGTGA 8 cut(s) 316, 398, 629, 710, 971, 1208, 2380, 2470
Hpy188I TCNGA 7 cut(s) 586, 1783, 1951, 1990, 2256, 2546, 2698
Hpy99I CGWCG 3 cut(s) 1872, 2121, 2307
HpyCH4III ACNGT 7 cut(s) 540, 1037, 2041, 2131, 2233, 2388, 2713
HpyCH4IV ACGT 5 cut(s) 847, 1273, 2116, 2305, 2706
HpyF10VI GCNNNNNNNGC 7 cut(s) 1142, 1151, 1160, 1342, 1847, 1970, 2361
HpySE526I ACGT 5 cut(s) 847, 1273, 2116, 2305, 2706
Hsp92I GRCGYC 2 cut(s) 238, 2116
Hsp92II CATG 7 cut(s) 274, 1496, 2002, 2029, 2182, 2263, 2635
HspAI GCGC 2 cut(s) 280, 654
Kpn2I TCCGGA 1 cut(s) 149
KpnI GGTACC 1 cut(s) 780
Ksp22I TGATCA 1 cut(s) 2256
Kzo9I GATC 8 cut(s) 74, 465, 814, 1353, 1426, 1632, 1783, 2256
LguI GCTCTTC 2 cut(s) 2596, 2763
LmnI GCTCC 6 cut(s) 725, 1133, 1139, 1214, 1341, 1342
Lsp1109I GCAGC 5 cut(s) 391, 641, 1150, 1154, 1957
LweI GCATC 6 cut(s) 433, 744, 1150, 2172, 2364, 2634
MabI ACCWGGT 1 cut(s) 904
MaeI CTAG 9 cut(s) 6, 87, 173, 249, 794, 1226, 1388, 1515, 2648
MaeII ACGT 5 cut(s) 847, 1273, 2116, 2305, 2706
MaeIII GTNAC 9 cut(s) 341, 540, 1604, 1705, 2078, 2345, 2368, 2530, 2707
MalI GATC 8 cut(s) 76, 467, 816, 1355, 1428, 1634, 1785, 2258
MbiI CCGCTC 1 cut(s) 1400
MboI GATC 8 cut(s) 74, 465, 814, 1353, 1426, 1632, 1783, 2256
MfeI CAATTG 4 cut(s) 924, 1155, 1449, 1905
MflI RGATCY 3 cut(s) 74, 814, 1353
MhlI GDGCHC 7 cut(s) 124, 264, 707, 1122, 1338, 1876, 2718
MluI ACGCGT 1 cut(s) 448
MlyI GAGTC 2 cut(s) 330, 2666
MmeI TCCRAC 8 cut(s) 703, 805, 1861, 1921, 1955, 2254, 2397, 2433
MroI TCCGGA 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 1566
MseI TTAA 9 cut(s) 165, 417, 438, 1058, 1127, 1175, 1287, 2661, 2767
MslI CAYNNNNRTG 2 cut(s) 2132, 2166
MspA1I CMGCKG 1 cut(s) 1772
MspI CCGG 5 cut(s) 36, 150, 235, 1339, 1369
MspR9I CCNGG 6 cut(s) 36, 192, 235, 906, 1340, 2067
MunI CAATTG 4 cut(s) 924, 1155, 1449, 1905
MvaI CCWGG 3 cut(s) 192, 906, 2067
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 7 cut(s) 1142, 1151, 1160, 1342, 1847, 1970, 2361
NciI CCSGG 3 cut(s) 36, 235, 1340
NdeII GATC 8 cut(s) 74, 465, 814, 1353, 1426, 1632, 1783, 2256
NheI GCTAGC 3 cut(s) 248, 793, 2647
NlaIII CATG 7 cut(s) 274, 1496, 2002, 2029, 2182, 2263, 2635
NlaIV GGNNCC 5 cut(s) 778, 816, 903, 1216, 1337
NmuCI GTSAC 5 cut(s) 341, 540, 1604, 2368, 2530
NspV TTCGAA 2 cut(s) 1570, 2496
OliI CACNNNNGTG 1 cut(s) 2132
PaeR7I CTCGAG 1 cut(s) 1476
PagI TCATGA 1 cut(s) 2259
PaqCI CACCTGC 1 cut(s) 133
PceI AGGCCT 2 cut(s) 190, 2593
PciSI GCTCTTC 2 cut(s) 2596, 2763
PcsI WCGNNNNNNNCGW 3 cut(s) 523, 1864, 1873
PdmI GAANNNNTTC 1 cut(s) 1566
PfeI GAWTC 5 cut(s) 429, 1778, 2002, 2693, 2741
PflFI GACNNNGTC 2 cut(s) 339, 2118
PflMI CCANNNNNTGG 2 cut(s) 862, 2582
PfoI TCCNGGA 1 cut(s) 1338
PkrI GCNGC 6 cut(s) 406, 631, 1144, 1165, 1399, 1972
PleI GAGTC 2 cut(s) 330, 2666
PpsI GAGTC 2 cut(s) 330, 2666
PpuMI RGGWCCY 2 cut(s) 412, 1697
Psp5II RGGWCCY 2 cut(s) 412, 1697
Psp6I CCWGG 3 cut(s) 190, 904, 2065
PspFI CCCAGC 3 cut(s) 662, 1258, 1592
PspGI CCWGG 3 cut(s) 190, 904, 2065
PspN4I GGNNCC 5 cut(s) 778, 816, 903, 1216, 1337
PspPI GGNCC 5 cut(s) 412, 565, 1618, 1697, 1928
PspPPI RGGWCCY 2 cut(s) 412, 1697
PsrI GAACNNNNNNTAC 2 cut(s) 2716, 2748
PstI CTGCAG 1 cut(s) 1206
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 3 cut(s) 74, 814, 1353
PsyI GACNNNGTC 2 cut(s) 339, 2118
PvuII CAGCTG 1 cut(s) 1772
RsaI GTAC 8 cut(s) 112, 358, 639, 676, 778, 1027, 1418, 2390
RsaNI GTAC 8 cut(s) 111, 357, 638, 675, 777, 1026, 1417, 2389
RseI CAYNNNNRTG 2 cut(s) 2132, 2166
SalI GTCGAC 1 cut(s) 94
SapI GCTCTTC 2 cut(s) 2596, 2763
SaqAI TTAA 9 cut(s) 165, 417, 438, 1058, 1127, 1175, 1287, 2661, 2767
SatI GCNGC 6 cut(s) 405, 630, 1143, 1164, 1398, 1971
Sau3AI GATC 8 cut(s) 74, 465, 814, 1353, 1426, 1632, 1783, 2256
Sau96I GGNCC 5 cut(s) 412, 565, 1618, 1697, 1928
ScaI AGTACT 1 cut(s) 2390
SchI GAGTC 2 cut(s) 330, 2666
ScrFI CCNGG 6 cut(s) 36, 192, 235, 906, 1340, 2067
SduI GDGCHC 7 cut(s) 124, 264, 707, 1122, 1338, 1876, 2718
SexAI ACCWGGT 1 cut(s) 904
SfaNI GCATC 6 cut(s) 433, 744, 1150, 2172, 2364, 2634
SfcI CTRYAG 1 cut(s) 1202
Sfr274I CTCGAG 1 cut(s) 1476
SfuI TTCGAA 2 cut(s) 1570, 2496
SinI GGWCC 4 cut(s) 412, 1618, 1697, 1928
SlaI CTCGAG 1 cut(s) 1476
SmiMI CAYNNNNRTG 2 cut(s) 2132, 2166
SmlI CTYRAG 5 cut(s) 641, 1476, 1622, 1793, 2195
SmoI CTYRAG 5 cut(s) 641, 1476, 1622, 1793, 2195
SseBI AGGCCT 2 cut(s) 190, 2593
SsiI CCGC 7 cut(s) 48, 871, 957, 1398, 1819, 2437, 2471
SspI AATATT 1 cut(s) 31
SspMI CTAG 9 cut(s) 6, 87, 173, 249, 794, 1226, 1388, 1515, 2648
StuI AGGCCT 2 cut(s) 190, 2593
StyD4I CCNGG 6 cut(s) 34, 190, 233, 904, 1338, 2065
StyI CCWWGG 2 cut(s) 408, 1548
TaaI ACNGT 7 cut(s) 540, 1037, 2041, 2131, 2233, 2388, 2713
TaiI ACGT 5 cut(s) 850, 1276, 2119, 2308, 2709
TaqI TCGA 7 cut(s) 95, 556, 1047, 1477, 1570, 1870, 2496
TaqII GACCGA 1 cut(s) 329
TatI WGTACW 3 cut(s) 637, 1416, 2388
TauI GCSGC 1 cut(s) 1400
TfiI GAWTC 5 cut(s) 429, 1778, 2002, 2693, 2741
Tru1I TTAA 9 cut(s) 165, 417, 438, 1058, 1127, 1175, 1287, 2661, 2767
Tru9I TTAA 9 cut(s) 165, 417, 438, 1058, 1127, 1175, 1287, 2661, 2767
TscAI CASTG 9 cut(s) 166, 549, 748, 1211, 1429, 2044, 2134, 2287, 2734
TseFI GTSAC 5 cut(s) 341, 540, 1604, 2368, 2530
TseI GCWGC 5 cut(s) 404, 629, 1142, 1163, 1970
Tsp45I GTSAC 5 cut(s) 341, 540, 1604, 2368, 2530
TspRI CASTG 9 cut(s) 166, 549, 748, 1211, 1429, 2044, 2134, 2287, 2734
Tth111I GACNNNGTC 2 cut(s) 339, 2118
Van91I CCANNNNNTGG 2 cut(s) 862, 2582
VneI GTGCAC 1 cut(s) 1118
VpaK11BI GGWCC 4 cut(s) 412, 1618, 1697, 1928
XapI RAATTY 4 cut(s) 646, 858, 2021, 2190
XbaI TCTAGA 2 cut(s) 172, 1225
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XhoI CTCGAG 1 cut(s) 1476
XmiI GTMKAC 2 cut(s) 95, 1373
XmnI GAANNNNTTC 1 cut(s) 1566
XspI CTAG 9 cut(s) 6, 87, 173, 249, 794, 1226, 1388, 1515, 2648
ZraI GACGTC 1 cut(s) 2117
ZrmI AGTACT 1 cut(s) 2390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.