FvH4_5g12270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010855
Physical Location & Seq
Forward (+)
6893906 .. 6894942
1037 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12270.t1

Sequence Viewer

Length: 447 bp
ATGGAGCAGACAAGAAAGAGGAGGGAGAAAGACGTCGACGACGAAACGAAGAAGAGCCGAGGAGGGGGCAAGAGGAAGAAGACAACGCAGCAGATGATCAAGGCCGACGATAACGAGGTGTCAGCGACGGAGGAGGAAGTGGAGGAGTTCTACGCGATAGTGAGGAGGATGCAGGCCGCGGTCAAGTACTTTGAGAAAGGTAATAATAACAAAAATATTCAGACCAATATTAACAATAATGGCGGGGATCAGGTGCGGTGGAGAGCGGCGCTGGAGTCTACTTTGCCGGTGGTTGATGCTGACGATCATGTAAAGAGTGCGACTAATTCGGATGACGATCATGATCATGATCAGTTGGTTGTTGTTACTGAACTAGCTGACAATAATGAGGTGGTTCTGGACCTGAACGAGGCGCCGGTTGAGGAGGCGGAGACTACTACTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.72

Weight (kDa)

4.7

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
AccB1I GGYRCC 1 cut(s) 412
AccBSI CCGCTC 1 cut(s) 266
AccI GTMKAC 2 cut(s) 36, 278
AccII CGCG 2 cut(s) 155, 179
AciI CCGC 6 cut(s) 177, 179, 243, 256, 266, 428
AclWI GGATC 1 cut(s) 255
AcyI GRCGYC 2 cut(s) 33, 413
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 2 cut(s) 64, 409
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
Alw26I GTCTC 1 cut(s) 425
AlwI GGATC 1 cut(s) 255
AoxI GGCC 2 cut(s) 102, 174
ApeKI GCWGC 1 cut(s) 88
AspLEI GCGC 2 cut(s) 271, 415
AspS9I GGNCC 1 cut(s) 400
AvaII GGWCC 1 cut(s) 400
BanI GGYRCC 1 cut(s) 412
BbsI GAAGAC 1 cut(s) 86
BbvI GCAGC 1 cut(s) 100
BclI TGATCA 3 cut(s) 96, 343, 349
BcoDI GTCTC 1 cut(s) 425
BfaI CTAG 1 cut(s) 374
BfoI RGCGCY 2 cut(s) 272, 416
BisI GCNGC 3 cut(s) 89, 177, 267
BlsI GCNGC 3 cut(s) 90, 178, 268
BmcAI AGTACT 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 400
BmiI GGNNCC 1 cut(s) 414
BmsI GCATC 2 cut(s) 159, 286
BpiI GAAGAC 1 cut(s) 86
BpmI CTGGAG 1 cut(s) 293
BsaBI GATNNNNATC 3 cut(s) 336, 342, 348
BsaHI GRCGYC 2 cut(s) 33, 413
BsaJI CCNNGG 2 cut(s) 58, 177
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 409
Bse118I RCCGGY 2 cut(s) 286, 415
Bse8I GATNNNNATC 3 cut(s) 336, 342, 348
BseDI CCNNGG 2 cut(s) 58, 177
BseGI GGATG 2 cut(s) 174, 337
BseJI GATNNNNATC 3 cut(s) 336, 342, 348
BseLI CCNNNNNNNGG 2 cut(s) 64, 409
BseRI GAGGAG 6 cut(s) 34, 75, 146, 158, 178, 437
BseXI GCAGC 1 cut(s) 100
Bsh1236I CGCG 2 cut(s) 155, 179
BshFI GGCC 2 cut(s) 104, 176
BshNI GGYRCC 1 cut(s) 412
BsiSI CCGG 2 cut(s) 287, 416
BslI CCNNNNNNNGG 2 cut(s) 64, 409
BsmAI GTCTC 1 cut(s) 425
BsnI GGCC 2 cut(s) 104, 176
Bsp143I GATC 6 cut(s) 96, 247, 304, 337, 343, 349
BspACI CCGC 6 cut(s) 177, 179, 243, 256, 266, 428
BspANI GGCC 2 cut(s) 104, 176
BspFNI CGCG 2 cut(s) 155, 179
BspHI TCATGA 2 cut(s) 340, 346
BspLI GGNNCC 1 cut(s) 414
BspPI GGATC 1 cut(s) 255
BspQI GCTCTTC 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 412
BsrBI CCGCTC 1 cut(s) 266
BsrFI RCCGGY 2 cut(s) 286, 415
BssAI RCCGGY 2 cut(s) 286, 415
BssECI CCNNGG 2 cut(s) 58, 177
BssMI GATC 6 cut(s) 96, 247, 304, 337, 343, 349
BssNI GRCGYC 2 cut(s) 33, 413
Bst6I CTCTTC 1 cut(s) 47
BstACI GRCGYC 2 cut(s) 33, 413
BstC8I GCNNGC 1 cut(s) 174
BstDSI CCRYGG 1 cut(s) 177
BstENI CCTNNNNNAGG 1 cut(s) 407
BstF5I GGATG 2 cut(s) 174, 337
BstFNI CGCG 2 cut(s) 155, 179
BstH2I RGCGCY 2 cut(s) 272, 416
BstHHI GCGC 2 cut(s) 271, 415
BstKTI GATC 6 cut(s) 99, 250, 307, 340, 346, 352
BstMAI GTCTC 1 cut(s) 425
BstMBI GATC 6 cut(s) 96, 247, 304, 337, 343, 349
BstUI CGCG 2 cut(s) 155, 179
BstV1I GCAGC 1 cut(s) 100
BstV2I GAAGAC 1 cut(s) 86
BsuRI GGCC 2 cut(s) 104, 176
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 2 cut(s) 174, 337
Cac8I GCNNGC 1 cut(s) 174
CciI TCATGA 2 cut(s) 340, 346
CfoI GCGC 2 cut(s) 271, 415
Cfr10I RCCGGY 2 cut(s) 286, 415
Cfr13I GGNCC 1 cut(s) 400
Cfr42I CCGCGG 1 cut(s) 180
Csp6I GTAC 1 cut(s) 187
CviAII CATG 3 cut(s) 308, 341, 347
CviJI RGCY 4 cut(s) 57, 104, 176, 377
CviKI_1 RGCY 4 cut(s) 57, 104, 176, 377
CviQI GTAC 1 cut(s) 187
DinI GGCGCC 1 cut(s) 414
DpnI GATC 6 cut(s) 98, 249, 306, 339, 345, 351
DpnII GATC 6 cut(s) 96, 247, 304, 337, 343, 349
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
EciI GGCGGA 1 cut(s) 443
Eco47I GGWCC 1 cut(s) 400
EcoNI CCTNNNNNAGG 1 cut(s) 407
EgeI GGCGCC 1 cut(s) 414
EheI GGCGCC 1 cut(s) 414
FaeI CATG 3 cut(s) 311, 344, 350
FaiI YATR 3 cut(s) 309, 342, 348
FatI CATG 3 cut(s) 307, 340, 346
FauI CCCGC 1 cut(s) 236
FbaI TGATCA 3 cut(s) 96, 343, 349
FblI GTMKAC 2 cut(s) 36, 278
Fnu4HI GCNGC 3 cut(s) 89, 177, 267
FokI GGATG 2 cut(s) 181, 344
Fsp4HI GCNGC 3 cut(s) 89, 177, 267
FspBI CTAG 1 cut(s) 374
GlaI GCGC 2 cut(s) 270, 414
GluI GCNGC 3 cut(s) 89, 177, 267
GsuI CTGGAG 1 cut(s) 293
HaeII RGCGCY 2 cut(s) 272, 416
HaeIII GGCC 2 cut(s) 104, 176
HapII CCGG 2 cut(s) 287, 416
HhaI GCGC 2 cut(s) 271, 415
Hin1I GRCGYC 2 cut(s) 33, 413
Hin1II CATG 3 cut(s) 311, 344, 350
Hin6I GCGC 2 cut(s) 269, 413
HinP1I GCGC 2 cut(s) 269, 413
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 1 cut(s) 275
HpaII CCGG 2 cut(s) 287, 416
Hpy166II GTNNAC 2 cut(s) 37, 279
Hpy188I TCNGA 2 cut(s) 222, 331
Hpy188III TCNNGA 3 cut(s) 341, 347, 398
Hpy8I GTNNAC 2 cut(s) 37, 279
Hpy99I CGWCG 5 cut(s) 38, 41, 44, 110, 130
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 1 cut(s) 172
HpySE526I ACGT 1 cut(s) 33
Hsp92I GRCGYC 2 cut(s) 33, 413
Hsp92II CATG 3 cut(s) 311, 344, 350
HspAI GCGC 2 cut(s) 269, 413
KasI GGCGCC 1 cut(s) 412
Ksp22I TGATCA 3 cut(s) 96, 343, 349
KspI CCGCGG 1 cut(s) 180
Kzo9I GATC 6 cut(s) 96, 247, 304, 337, 343, 349
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 158, 236, 257, 300, 383, 416, 429
Lsp1109I GCAGC 1 cut(s) 100
LweI GCATC 2 cut(s) 159, 286
MaeI CTAG 1 cut(s) 374
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 364
MalI GATC 6 cut(s) 98, 249, 306, 339, 345, 351
MbiI CCGCTC 1 cut(s) 266
MboI GATC 6 cut(s) 96, 247, 304, 337, 343, 349
MboII GAAGA 4 cut(s) 61, 64, 88, 91
MluCI AATT 1 cut(s) 325
Mly113I GGCGCC 1 cut(s) 413
MlyI GAGTC 1 cut(s) 284
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 2 cut(s) 287, 416
MvnI CGCG 2 cut(s) 155, 179
NarI GGCGCC 1 cut(s) 413
NdeII GATC 6 cut(s) 96, 247, 304, 337, 343, 349
NlaIII CATG 3 cut(s) 311, 344, 350
NlaIV GGNNCC 1 cut(s) 414
NmeAIII GCCGAG 1 cut(s) 83
PagI TCATGA 2 cut(s) 340, 346
PciSI GCTCTTC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PkrI GCNGC 3 cut(s) 90, 178, 268
PleI GAGTC 1 cut(s) 283
PluTI GGCGCC 1 cut(s) 416
PpsI GAGTC 1 cut(s) 283
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 1 cut(s) 400
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
RseI CAYNNNNRTG 1 cut(s) 345
SacII CCGCGG 1 cut(s) 180
SalI GTCGAC 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 3 cut(s) 89, 177, 267
Sau3AI GATC 6 cut(s) 96, 247, 304, 337, 343, 349
Sau96I GGNCC 1 cut(s) 400
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 1 cut(s) 284
SetI ASST 7 cut(s) 36, 120, 202, 255, 379, 393, 405
SfaNI GCATC 2 cut(s) 159, 286
SfoI GGCGCC 1 cut(s) 414
Sfr303I CCGCGG 1 cut(s) 180
SgrBI CCGCGG 1 cut(s) 180
SgrDI CGTCGACG 1 cut(s) 35
SinI GGWCC 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 345
Sse9I AATT 1 cut(s) 325
SsiI CCGC 6 cut(s) 177, 179, 243, 256, 266, 428
SspDI GGCGCC 1 cut(s) 412
SspI AATATT 2 cut(s) 217, 229
SspMI CTAG 1 cut(s) 374
TaiI ACGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 36
TasI AATT 1 cut(s) 325
TatI WGTACW 1 cut(s) 186
TauI GCSGC 2 cut(s) 179, 269
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TseI GCWGC 1 cut(s) 88
TspGWI ACGGA 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 400
XagI CCTNNNNNAGG 1 cut(s) 407
XmiI GTMKAC 2 cut(s) 36, 278
XspI CTAG 1 cut(s) 374
ZraI GACGTC 1 cut(s) 34
ZrmI AGTACT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.