Rroxscaffold_3G00269070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010855
Physical Location & Seq
Forward (+)
62012763 .. 62013167
405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269070.1

Sequence Viewer

Length: 405 bp
ATGGACAAGAGCGGAGGAGGAGGAGGGAGGAGGAAGAAGACAGCGCAGCAGGTGATCAAGGCTGACAAGGAAGAAGCGGTGGCCGCGACGGAGGAGGAGGTGGAGGAATTCTACGCCATAGTGAGGAGGATGCAGGCGGCGGTTAAGTACTTCGAGAAAGGTAATAACAACAATATTCACGCCAATATAAACAATGGCGGGGATCTGGTGCGGTGGAGAGTGGCGCTGGAGTCTACTCCGCCGGTGGTTGCCGTCGCCGATGCGGATGATGATGTTGCTGACGATCATGTAAAGAGTGCGACTTCCGATGATCATCGTGAGCAGTTGGTTGTTCAACTAGCTGACAATAATGGAGTTCTGGACTTGAACGAGGCGCCGGGTGAGGAGGCGGAGACTACTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

134

Amino Acids

14.52

Weight (kDa)

4.57

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 40
Acc36I ACCTGC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 373
AccBSI CCGCTC 1 cut(s) 12
AccI GTMKAC 1 cut(s) 233
AccII CGCG 1 cut(s) 86
AclWI GGATC 1 cut(s) 210
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 374
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 2 cut(s) 335, 367
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 1 cut(s) 210
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 1 cut(s) 107
AspLEI GCGC 3 cut(s) 46, 226, 376
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 2 cut(s) 64, 392
BanI GGYRCC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 58
BceAI ACGGC 1 cut(s) 236
BclI TGATCA 2 cut(s) 54, 310
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 1 cut(s) 338
BfoI RGCGCY 2 cut(s) 227, 377
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 3 cut(s) 47, 84, 138
BlsI GCNGC 3 cut(s) 48, 85, 139
BmcAI AGTACT 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 378
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 1 cut(s) 378
BmsI GCATC 2 cut(s) 120, 250
BpiI GAAGAC 1 cut(s) 44
BpmI CTGGAG 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 402
BsaBI GATNNNNATC 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 374
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse118I RCCGGY 1 cut(s) 241
Bse8I GATNNNNATC 1 cut(s) 312
BseGI GGATG 2 cut(s) 135, 271
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 123
BseRI GAGGAG 8 cut(s) 30, 33, 36, 43, 107, 110, 139, 398
BseXI GCAGC 1 cut(s) 58
Bsh1236I CGCG 1 cut(s) 86
BshFI GGCC 1 cut(s) 83
BshNI GGYRCC 1 cut(s) 373
BsiSI CCGG 2 cut(s) 242, 377
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 386
BsnI GGCC 1 cut(s) 83
Bsp143I GATC 4 cut(s) 54, 202, 283, 310
BspANI GGCC 1 cut(s) 83
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 375
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 241
BssAI RCCGGY 1 cut(s) 241
BssMI GATC 4 cut(s) 54, 202, 283, 310
BssNI GRCGYC 1 cut(s) 374
BstACI GRCGYC 1 cut(s) 374
BstBAI YACGTR 1 cut(s) 402
BstC8I GCNNGC 1 cut(s) 135
BstF5I GGATG 2 cut(s) 135, 271
BstFNI CGCG 1 cut(s) 86
BstH2I RGCGCY 2 cut(s) 227, 377
BstHHI GCGC 3 cut(s) 46, 226, 376
BstKTI GATC 4 cut(s) 57, 205, 286, 313
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 4 cut(s) 54, 202, 283, 310
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 376
BstSNI TACGTA 1 cut(s) 402
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 1 cut(s) 58
BstV2I GAAGAC 1 cut(s) 44
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BsuRI GGCC 1 cut(s) 83
BtsCI GGATG 2 cut(s) 135, 271
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 1 cut(s) 135
CfoI GCGC 3 cut(s) 46, 226, 376
Cfr10I RCCGGY 1 cut(s) 241
Csp6I GTAC 1 cut(s) 148
CviAII CATG 1 cut(s) 287
CviJI RGCY 3 cut(s) 62, 83, 341
CviKI_1 RGCY 3 cut(s) 62, 83, 341
CviQI GTAC 1 cut(s) 148
DinI GGCGCC 1 cut(s) 375
DpnI GATC 4 cut(s) 56, 204, 285, 312
DpnII GATC 4 cut(s) 54, 202, 283, 310
EaeI YGGCCR 1 cut(s) 81
EciI GGCGGA 2 cut(s) 228, 404
Eco105I TACGTA 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 107
EgeI GGCGCC 1 cut(s) 375
EheI GGCGCC 1 cut(s) 375
FaeI CATG 1 cut(s) 290
FaiI YATR 3 cut(s) 119, 188, 288
FatI CATG 1 cut(s) 286
FauI CCCGC 1 cut(s) 191
FbaI TGATCA 2 cut(s) 54, 310
FblI GTMKAC 1 cut(s) 233
Fnu4HI GCNGC 3 cut(s) 47, 84, 138
FokI GGATG 2 cut(s) 142, 278
Fsp4HI GCNGC 3 cut(s) 47, 84, 138
FspBI CTAG 1 cut(s) 338
GlaI GCGC 3 cut(s) 45, 225, 375
GluI GCNGC 3 cut(s) 47, 84, 138
GsuI CTGGAG 1 cut(s) 248
HaeII RGCGCY 2 cut(s) 227, 377
HaeIII GGCC 1 cut(s) 83
HapII CCGG 2 cut(s) 242, 377
HhaI GCGC 3 cut(s) 46, 226, 376
Hin1I GRCGYC 1 cut(s) 374
Hin1II CATG 1 cut(s) 290
Hin6I GCGC 3 cut(s) 44, 224, 374
HinP1I GCGC 3 cut(s) 44, 224, 374
HinfI GANTC 1 cut(s) 230
HpaII CCGG 2 cut(s) 242, 377
HphI GGTGA 2 cut(s) 64, 392
Hpy166II GTNNAC 1 cut(s) 234
Hpy188I TCNGA 1 cut(s) 307
Hpy188III TCNNGA 3 cut(s) 154, 317, 359
Hpy8I GTNNAC 1 cut(s) 234
Hpy99I CGWCG 2 cut(s) 91, 257
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 1 cut(s) 133
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpySE526I ACGT 1 cut(s) 401
Hsp92I GRCGYC 1 cut(s) 374
Hsp92II CATG 1 cut(s) 290
HspAI GCGC 3 cut(s) 44, 224, 374
KasI GGCGCC 1 cut(s) 373
Ksp22I TGATCA 2 cut(s) 54, 310
Kzo9I GATC 4 cut(s) 54, 202, 283, 310
LpnPI CCDG 7 cut(s) 35, 119, 191, 212, 255, 344, 390
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 2 cut(s) 120, 250
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 401
MalI GATC 4 cut(s) 56, 204, 285, 312
MbiI CCGCTC 1 cut(s) 12
MboI GATC 4 cut(s) 54, 202, 283, 310
MboII GAAGA 3 cut(s) 46, 49, 83
MflI RGATCY 1 cut(s) 202
MluCI AATT 1 cut(s) 107
Mly113I GGCGCC 1 cut(s) 374
MlyI GAGTC 1 cut(s) 239
MseI TTAA 1 cut(s) 144
MspI CCGG 2 cut(s) 242, 377
MspR9I CCNGG 1 cut(s) 378
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 1 cut(s) 83
NarI GGCGCC 1 cut(s) 374
NciI CCSGG 1 cut(s) 378
NdeII GATC 4 cut(s) 54, 202, 283, 310
NlaIII CATG 1 cut(s) 290
NlaIV GGNNCC 1 cut(s) 375
PaqCI CACCTGC 1 cut(s) 40
PkrI GCNGC 3 cut(s) 48, 85, 139
PleI GAGTC 1 cut(s) 238
PluTI GGCGCC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 238
Ppu21I YACGTR 1 cut(s) 402
PspN4I GGNNCC 1 cut(s) 375
PsuI RGATCY 1 cut(s) 202
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 3 cut(s) 47, 84, 138
Sau3AI GATC 4 cut(s) 54, 202, 283, 310
ScaI AGTACT 1 cut(s) 149
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 378
SetI ASST 5 cut(s) 54, 102, 163, 343, 404
SfaNI GCATC 2 cut(s) 120, 250
SfoI GGCGCC 1 cut(s) 375
SgrAI CRCCGGYG 1 cut(s) 241
SnaBI TACGTA 1 cut(s) 402
Sse9I AATT 1 cut(s) 107
SspDI GGCGCC 1 cut(s) 373
SspI AATATT 1 cut(s) 175
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 1 cut(s) 376
TaiI ACGT 1 cut(s) 404
TaqI TCGA 1 cut(s) 153
TasI AATT 1 cut(s) 107
TatI WGTACW 1 cut(s) 147
TauI GCSGC 2 cut(s) 86, 140
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseI GCWGC 1 cut(s) 46
TspGWI ACGGA 1 cut(s) 104
XapI RAATTY 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 233
XspI CTAG 1 cut(s) 338
ZrmI AGTACT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.