FvH4_6g06040

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008144
Physical Location & Seq
Forward (+)
3442543 .. 3443721
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06040.t1

Sequence Viewer

Length: 1179 bp
ATGGCTTTTCAAAATAACCTTGCTATCATTGCCGTTTTATCCATCATCTTGGGGGCAATGGCACAACCCTCAGCCACGTCCAACGACGAAGTCGATCCCTCTGCATTTTTGAAAACATTCGAGCAATGGATGGCACAGTTCGGACGCACTTACGATGACATTGCAGAGAAACAAAGCCGTCTCGCCATTTTTGTGAAGAACTTGTTATTTGTGAATGACTTCGACAACCAAGGAAACAAGACTTACAAGTTAAACATCAACTTGTTTTCCGATATGACCAATGAAGAATTCCTCGGACTTTACACTGGATTTCAAGCTCCCAATGACACCTTAAGCTCGAGCTCATTACTTCGGCAACGTAATACCAAATCTTTTAGGTATCAAGACCTGTCGGAAGCTGATGTTCCGGCTAGAGTTGACTGGAGGGAACAAGGTGCTGTTACCCCCGTCAAGGAACAAGGACAATGCAGTAAGAGAACCACCGTATCTCAAACCTTAATCAATTTCTTGTACATTTCTCCATGTAAACTTATGAAATTTTCAACACATACGCAGGTTCTTGTTTGGGCGTTTTCAACAGTGGCAACAATTGAGGGGATTACCAAAATCAAAAGTGACAAATTGATTTCACTGTCCGAGCAACAACTGGTGGACTGTAACAGAGATCAGATTAACAGAGGCTGCCGTGGTGGTGACATGGAAGATGCGTTTAAATACATAGAGGAAAACGGAGGAATCACTACTGAAGAAAACTACCAATACAAGGGTACAGACGAAACATGTGACACTACTAAGACGAATGAGTACGCTGCCAAGATAACCGGCTATGAAATGGTTCCTCCCAATAGCGAAAATGATCTGCTTAAGGCTGTGGCCATGCAACCAGTCTCGGTTGGCATTGATGCCAATGGAGAAGAGTTTAGGGCATACGCAAGTGGGGTATTTTCCGGCAACTGTGGCACAAACCTAAACCACGCTGTTACAATTGTTGGGTATGGAACGACTGAAGAAGGCACCGACTATTGGTTAGTGAAGAATTCATGGGGCGAAACCTGGGGTGAAAAGGGATACGTGAGAATTCTTCGGAATGCTGGTCCTCCGGAAGGTTTGTGTGGCATTGCTATGTATGCTTCCTATCCAACTGCATCAGCTATGGCACCCTCTCCCTCAGTATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

393

Amino Acids

43.4

Weight (kDa)

4.81

Isoelectric Point (pI)

31.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 40 - 97 1.5e-17 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 189 - 381 4.9e-68 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 544
AccB1I GGYRCC 2 cut(s) 1013, 1156
AccIII TCCGGA 1 cut(s) 1099
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 1 cut(s) 873
AcsI RAATTY 4 cut(s) 287, 536, 1036, 1077
AcuI CTGAAG 2 cut(s) 765, 1026
AfaI GTAC 3 cut(s) 512, 769, 806
AfiI CCNNNNNNNGG 4 cut(s) 451, 763, 1023, 1103
AflII CTTAAG 2 cut(s) 331, 863
AflIII ACRYGT 1 cut(s) 779
AgsI TTSAA 5 cut(s) 11, 112, 314, 543, 576
AjiI CACGTC 1 cut(s) 78
AjnI CCWGG 1 cut(s) 1052
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 5 cut(s) 317, 336, 342, 398, 1151
AluI AGCT 5 cut(s) 317, 336, 342, 398, 1151
Alw21I GWGCWC 1 cut(s) 344
Alw26I GTCTC 2 cut(s) 185, 892
AlwI GGATC 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 681
Ama87I CYCGRG 1 cut(s) 337
Aor13HI TCCGGA 1 cut(s) 1099
AoxI GGCC 1 cut(s) 873
ApeKI GCWGC 2 cut(s) 681, 809
ApoI RAATTY 4 cut(s) 287, 536, 1036, 1077
Asp700I GAANNNNTTC 3 cut(s) 116, 218, 834
AspS9I GGNCC 1 cut(s) 1094
AsuHPI GGTGA 2 cut(s) 704, 1070
AvaI CYCGRG 1 cut(s) 337
AvaII GGWCC 1 cut(s) 1094
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BalI TGGCCA 1 cut(s) 875
BanI GGYRCC 2 cut(s) 1013, 1156
BanII GRGCYC 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 344
BbvCI CCTCAGC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 668, 796
BccI CCATC 2 cut(s) 50, 124
BceAI ACGGC 3 cut(s) 17, 162, 669
BcgI CGANNNNNNTGC 4 cut(s) 83, 117, 143, 177
BciT130I CCWGG 1 cut(s) 1054
BciVI GTATCC 1 cut(s) 1061
BcoDI GTCTC 2 cut(s) 185, 892
BfaI CTAG 1 cut(s) 411
BfrI CTTAAG 2 cut(s) 331, 863
BfuAI ACCTGC 1 cut(s) 544
BfuI GTATCC 1 cut(s) 1061
BisI GCNGC 2 cut(s) 682, 810
BlsI GCNGC 2 cut(s) 683, 811
Bme1390I CCNGG 1 cut(s) 1054
Bme18I GGWCC 1 cut(s) 1094
BmeT110I CYCGRG 1 cut(s) 337
BmgBI CACGTC 1 cut(s) 78
BmgT120I GGNCC 1 cut(s) 1094
BmiI GGNNCC 3 cut(s) 837, 1015, 1158
BmrFI CCNGG 1 cut(s) 1054
BmsI GCATC 3 cut(s) 694, 892, 1154
BpmI CTGGAG 1 cut(s) 442
Bpu10I CCTNAGC 1 cut(s) 70
BsaAI YACGTR 1 cut(s) 1072
BsaJI CCNNGG 4 cut(s) 229, 292, 685, 1053
BsaWI WCCGGW 1 cut(s) 1099
Bsc4I CCNNNNNNNGG 4 cut(s) 451, 763, 1023, 1103
Bse118I RCCGGY 1 cut(s) 821
Bse1I ACTGG 4 cut(s) 310, 425, 651, 884
Bse3DI GCAATG 5 cut(s) 27, 63, 131, 159, 1116
BseAI TCCGGA 1 cut(s) 1099
BseBI CCWGG 1 cut(s) 1054
BseDI CCNNGG 4 cut(s) 229, 292, 685, 1053
BseGI GGATG 1 cut(s) 135
BseLI CCNNNNNNNGG 4 cut(s) 451, 763, 1023, 1103
BseMI GCAATG 5 cut(s) 27, 63, 131, 159, 1116
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 4 cut(s) 310, 425, 651, 884
BseXI GCAGC 2 cut(s) 668, 796
BshFI GGCC 1 cut(s) 875
BshNI GGYRCC 2 cut(s) 1013, 1156
BsiHKAI GWGCWC 1 cut(s) 344
BsiHKCI CYCGRG 1 cut(s) 337
BsiSI CCGG 4 cut(s) 407, 822, 948, 1100
BslI CCNNNNNNNGG 4 cut(s) 451, 763, 1023, 1103
BsmAI GTCTC 2 cut(s) 185, 892
BsmBI CGTCTC 1 cut(s) 185
BsmI GAATGC 1 cut(s) 1093
BsnI GGCC 1 cut(s) 875
BsoBI CYCGRG 1 cut(s) 337
Bsp1286I GDGCHC 1 cut(s) 344
Bsp13I TCCGGA 1 cut(s) 1099
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 3 cut(s) 94, 664, 856
BspANI GGCC 1 cut(s) 875
BspCNI CTCAG 1 cut(s) 83
BspEI TCCGGA 1 cut(s) 1099
BspLI GGNNCC 3 cut(s) 837, 1015, 1158
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 1 cut(s) 89
BspT107I GGYRCC 2 cut(s) 1013, 1156
BspTI CTTAAG 2 cut(s) 331, 863
BsrDI GCAATG 5 cut(s) 27, 63, 131, 159, 1116
BsrFI RCCGGY 1 cut(s) 821
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 4 cut(s) 310, 425, 651, 884
BssAI RCCGGY 1 cut(s) 821
BssECI CCNNGG 4 cut(s) 229, 292, 685, 1053
BssMI GATC 3 cut(s) 94, 664, 856
BssT1I CCWWGG 1 cut(s) 229
Bst2UI CCWGG 1 cut(s) 1054
Bst4CI ACNGT 6 cut(s) 138, 484, 580, 633, 656, 956
Bst6I CTCTTC 1 cut(s) 909
BstAFI CTTAAG 2 cut(s) 331, 863
BstAUI TGTACA 1 cut(s) 510
BstBAI YACGTR 1 cut(s) 1072
BstDEI CTNAG 3 cut(s) 70, 792, 1168
BstDSI CCRYGG 1 cut(s) 685
BstENI CCTNNNNNAGG 1 cut(s) 1101
BstF5I GGATG 1 cut(s) 135
BstKTI GATC 3 cut(s) 97, 667, 859
BstMAI GTCTC 2 cut(s) 185, 892
BstMBI GATC 3 cut(s) 94, 664, 856
BstMWI GCNNNNNNNGC 3 cut(s) 29, 957, 1127
BstNI CCWGG 1 cut(s) 1054
BstNSI RCATGY 1 cut(s) 783
BstSCI CCNGG 1 cut(s) 1052
BstV1I GCAGC 2 cut(s) 668, 796
BstXI CCANNNNNNTGG 1 cut(s) 49
BsuI GTATCC 1 cut(s) 1061
BsuRI GGCC 1 cut(s) 875
BtgI CCRYGG 1 cut(s) 685
BtrI CACGTC 1 cut(s) 78
BtsCI GGATG 1 cut(s) 135
BtsIMutI CAGTG 3 cut(s) 303, 585, 629
BveI ACCTGC 1 cut(s) 544
CaiI CAGNNNCTG 1 cut(s) 681
Cfr10I RCCGGY 1 cut(s) 821
Cfr13I GGNCC 1 cut(s) 1094
CseI GACGC 1 cut(s) 153
Csp6I GTAC 3 cut(s) 511, 768, 805
CviAII CATG 5 cut(s) 522, 697, 780, 877, 1041
CviQI GTAC 3 cut(s) 511, 768, 805
DdeI CTNAG 3 cut(s) 70, 792, 1168
DpnI GATC 3 cut(s) 96, 666, 858
DpnII GATC 3 cut(s) 94, 664, 856
DraI TTTAAA 1 cut(s) 712
EaeI YGGCCR 1 cut(s) 873
Eam1104I CTCTTC 1 cut(s) 909
EarI CTCTTC 1 cut(s) 909
Ecl136II GAGCTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 344
Eco47I GGWCC 1 cut(s) 1094
Eco53kI GAGCTC 1 cut(s) 342
Eco57I CTGAAG 2 cut(s) 765, 1026
Eco88I CYCGRG 1 cut(s) 337
EcoICRI GAGCTC 1 cut(s) 342
EcoNI CCTNNNNNAGG 1 cut(s) 1101
EcoRI GAATTC 3 cut(s) 287, 1036, 1077
EcoRII CCWGG 1 cut(s) 1052
EcoT14I CCWWGG 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 344
ErhI CCWWGG 1 cut(s) 229
Esp3I CGTCTC 1 cut(s) 185
FaeI CATG 5 cut(s) 525, 700, 783, 880, 1044
FatI CATG 5 cut(s) 521, 696, 779, 876, 1040
Fnu4HI GCNGC 2 cut(s) 682, 810
FokI GGATG 1 cut(s) 142
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 2 cut(s) 682, 810
FspBI CTAG 1 cut(s) 411
GluI GCNGC 2 cut(s) 682, 810
GsuI CTGGAG 1 cut(s) 442
HaeIII GGCC 1 cut(s) 875
HapII CCGG 4 cut(s) 407, 822, 948, 1100
HgaI GACGC 1 cut(s) 153
Hin1II CATG 5 cut(s) 525, 700, 783, 880, 1044
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 1 cut(s) 735
HpaII CCGG 4 cut(s) 407, 822, 948, 1100
HphI GGTGA 2 cut(s) 704, 1070
Hpy166II GTNNAC 3 cut(s) 418, 527, 652
Hpy188I TCNGA 7 cut(s) 143, 271, 296, 394, 637, 669, 1086
Hpy188III TCNNGA 2 cut(s) 383, 1100
Hpy8I GTNNAC 3 cut(s) 418, 527, 652
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 2 cut(s) 1004, 1097
HpyCH4III ACNGT 6 cut(s) 138, 484, 580, 633, 656, 956
HpyCH4IV ACGT 3 cut(s) 77, 358, 1071
HpyCH4V TGCA 5 cut(s) 104, 164, 468, 880, 1145
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 957, 1127
HpyF3I CTNAG 3 cut(s) 70, 792, 1168
HpySE526I ACGT 3 cut(s) 77, 358, 1071
Hsp92II CATG 5 cut(s) 525, 700, 783, 880, 1044
Kpn2I TCCGGA 1 cut(s) 1099
Kzo9I GATC 3 cut(s) 94, 664, 856
LmnI GCTCC 1 cut(s) 322
Lsp1109I GCAGC 2 cut(s) 668, 796
LweI GCATC 3 cut(s) 694, 892, 1154
MaeI CTAG 1 cut(s) 411
MaeII ACGT 3 cut(s) 77, 358, 1071
MaeIII GTNAC 6 cut(s) 439, 614, 656, 692, 782, 979
MalI GATC 3 cut(s) 96, 666, 858
MboI GATC 3 cut(s) 94, 664, 856
MboII GAAGA 8 cut(s) 208, 296, 713, 758, 926, 1019, 1045, 1073
MfeI CAATTG 2 cut(s) 588, 984
MhlI GDGCHC 1 cut(s) 344
MlsI TGGCCA 1 cut(s) 875
MluCI AATT 8 cut(s) 287, 502, 536, 588, 620, 984, 1036, 1077
MluNI TGGCCA 1 cut(s) 875
MmeI TCCRAC 3 cut(s) 105, 372, 1163
Mox20I TGGCCA 1 cut(s) 875
MroI TCCGGA 1 cut(s) 1099
MroXI GAANNNNTTC 3 cut(s) 116, 218, 834
MscI TGGCCA 1 cut(s) 875
MseI TTAA 6 cut(s) 251, 332, 497, 672, 711, 864
MslI CAYNNNNRTG 2 cut(s) 191, 1121
Msp20I TGGCCA 1 cut(s) 875
MspCI CTTAAG 2 cut(s) 331, 863
MspI CCGG 4 cut(s) 407, 822, 948, 1100
MspR9I CCNGG 1 cut(s) 1054
MunI CAATTG 2 cut(s) 588, 984
Mva1269I GAATGC 1 cut(s) 1093
MvaI CCWGG 1 cut(s) 1054
MwoI GCNNNNNNNGC 3 cut(s) 29, 957, 1127
NdeII GATC 3 cut(s) 94, 664, 856
NlaIII CATG 5 cut(s) 525, 700, 783, 880, 1044
NlaIV GGNNCC 3 cut(s) 837, 1015, 1158
NmuCI GTSAC 3 cut(s) 614, 692, 782
NspI RCATGY 1 cut(s) 783
PaeR7I CTCGAG 1 cut(s) 337
PciI ACATGT 1 cut(s) 779
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 1093
PdmI GAANNNNTTC 3 cut(s) 116, 218, 834
PfeI GAWTC 1 cut(s) 735
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 683, 811
Ppu21I YACGTR 1 cut(s) 1072
PscI ACATGT 1 cut(s) 779
Psp124BI GAGCTC 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 1052
PspGI CCWGG 1 cut(s) 1052
PspN4I GGNNCC 3 cut(s) 837, 1015, 1158
PspPI GGNCC 1 cut(s) 1094
PspXI VCTCGAGB 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 681
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 3 cut(s) 512, 769, 806
RsaNI GTAC 3 cut(s) 511, 768, 805
RseI CAYNNNNRTG 2 cut(s) 191, 1121
SacI GAGCTC 1 cut(s) 344
SaqAI TTAA 6 cut(s) 251, 332, 497, 672, 711, 864
SatI GCNGC 2 cut(s) 682, 810
Sau3AI GATC 3 cut(s) 94, 664, 856
Sau96I GGNCC 1 cut(s) 1094
ScrFI CCNGG 1 cut(s) 1054
SduI GDGCHC 1 cut(s) 344
SfaNI GCATC 3 cut(s) 694, 892, 1154
Sfr274I CTCGAG 1 cut(s) 337
SinI GGWCC 1 cut(s) 1094
SlaI CTCGAG 1 cut(s) 337
SmiMI CAYNNNNRTG 2 cut(s) 191, 1121
SmlI CTYRAG 3 cut(s) 331, 337, 863
SmoI CTYRAG 3 cut(s) 331, 337, 863
Sse9I AATT 8 cut(s) 287, 502, 536, 588, 620, 984, 1036, 1077
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 1 cut(s) 344
StyD4I CCNGG 1 cut(s) 1052
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 6 cut(s) 138, 484, 580, 633, 656, 956
TaiI ACGT 3 cut(s) 80, 361, 1074
TaqI TCGA 4 cut(s) 93, 120, 222, 338
TasI AATT 8 cut(s) 287, 502, 536, 588, 620, 984, 1036, 1077
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 1 cut(s) 735
Tru1I TTAA 6 cut(s) 251, 332, 497, 672, 711, 864
Tru9I TTAA 6 cut(s) 251, 332, 497, 672, 711, 864
TscAI CASTG 3 cut(s) 310, 585, 636
TseFI GTSAC 3 cut(s) 614, 692, 782
TseI GCWGC 2 cut(s) 681, 809
Tsp45I GTSAC 3 cut(s) 614, 692, 782
TspDTI ATGAA 4 cut(s) 297, 548, 843, 1029
TspGWI ACGGA 1 cut(s) 744
TspRI CASTG 3 cut(s) 310, 585, 636
Tth111I GACNNNGTC 1 cut(s) 89
Vha464I CTTAAG 2 cut(s) 331, 863
VpaK11BI GGWCC 1 cut(s) 1094
XagI CCTNNNNNAGG 1 cut(s) 1101
XapI RAATTY 4 cut(s) 287, 536, 1036, 1077
XceI RCATGY 1 cut(s) 783
XhoI CTCGAG 1 cut(s) 337
XmnI GAANNNNTTC 3 cut(s) 116, 218, 834
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.