RchiOBHm_Chr3g0455061

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008144
Physical Location & Seq
Forward (+)
4934072 .. 4935344
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42204

Sequence Viewer

Length: 1176 bp
ATGCTCTCTCCTTCACAGCTTGCTAAATCAAAGTCATTTAGTATCAGAGACCCCTCATTGATATATACCAAGTGTAGTACTGCAATGGCTTTTCAAAATAACCTTGTTATAATTGCCATTTTATCTATCATCTTGGGGGCAATGGCACAACCCCCAGCCACGTCCAACGATGAAGTTGATCCCTCTACATTTTTGAAAACATTTGAGCAATGGATGGCACAGTTCGGACGCACTTACGGTGACAATGCAGAGAAACAAAGGCGTCTCACCATATTCGTGAAGAACTTGTTATTTGTAAATAACTTCAACAGCCAAGGGAATAAGACTTACAAGTTAAGCGTCAACTTGTTTTCTGATATGGCCAATGAAGAATTTCTCGGACTTTACACTGGATTTCAAGCTCCCAATGTCACCTTAAACTCAAGCTCATTCCGTGGACAACGTAATATCAAATCTTTTAGGTACCAAGACCTGTCTGAAACTGATGTTCCGACTAGAATTGACTGGAGGGAACAAGGTGCTGTTACCCCCGTCAAGGAACAAGGACAATGCCGTTCTTGTTGGGCATTTTCAGCAGTGGCAGCTATTGAGGGCATTACCAAAATCAATAGTAACCAATTGATTTCACTGTCCGAGCAACAACTTGTGGACTGTAACAGAGATCAAATTAACAAGGGCTGCGGTGGTGGTAATATGGAAGATGCCTTTAAATACATAAATGACAACGGAGGAATCACTAGTGAAGAAAACTACCAATACAAGGGTGCAGAAGAGACATGTGACACTACTAAGACGACTGAATATGCTGCCAAGATAAACGGTTATGAAATGGTTCCTCCCAATAGCGAAAATGATCTACTGAAGGCCGTGGCCAAGCAACCAGTCTCAGTTGCCATTGATGCCGATGGAGATGAATTTAGGGCATACGCAAGTGGGGTATTTTCTGGTAATTGCGGGACAAACCTAACCCATGCTGTTACAATCGTTGGGTTCGGAACGACTGAAGAAGGCACCAACTATTGGTTAGTGAAGAATTCATGGGGCGAAACCTGGGGTGAAAAGGGCTACGTGAGAATTCTTCGAAATGCTGGTCCTCCAGGAGGTTTGTGTGGCATTGCTATGTACCCTTCTTATCCAACTGCATCAGCTATGGCACCCGCTCCCTCACCACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

391

Amino Acids

42.86

Weight (kDa)

5.15

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 68 - 125 2.1e-18 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 163 - 380 4.1e-80 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 110
Acc65I GGTACC 1 cut(s) 462
AccB1I GGYRCC 3 cut(s) 462, 1010, 1153
AccB7I CCANNNNNTGG 1 cut(s) 1020
AccBSI CCGCTC 1 cut(s) 1160
AciI CCGC 3 cut(s) 681, 954, 1158
AclWI GGATC 1 cut(s) 173
AcoI YGGCCR 2 cut(s) 360, 870
AcsI RAATTY 4 cut(s) 371, 914, 1033, 1074
AcuI CTGAAG 2 cut(s) 881, 1023
AcyI GRCGYC 1 cut(s) 262
AfaI GTAC 3 cut(s) 79, 464, 1124
AfiI CCNNNNNNNGG 4 cut(s) 535, 760, 1020, 1100
AflIII ACRYGT 1 cut(s) 776
AgsI TTSAA 4 cut(s) 95, 196, 307, 398
AhlI ACTAGT 1 cut(s) 737
AjiI CACGTC 1 cut(s) 162
AjnI CCWGG 2 cut(s) 1049, 1096
AluBI AGCT 5 cut(s) 19, 401, 426, 584, 1148
AluI AGCT 5 cut(s) 19, 401, 426, 584, 1148
Alw26I GTCTC 4 cut(s) 42, 269, 767, 889
AlwI GGATC 1 cut(s) 173
AoxI GGCC 3 cut(s) 360, 864, 870
ApeKI GCWGC 3 cut(s) 581, 678, 806
ApoI RAATTY 4 cut(s) 371, 914, 1033, 1074
ArsI GACNNNNNNTTYG 2 cut(s) 1075, 1107
Asp700I GAANNNNTTC 2 cut(s) 372, 831
Asp718I GGTACC 1 cut(s) 462
AspS9I GGNCC 1 cut(s) 1091
AsuHPI GGTGA 5 cut(s) 251, 259, 403, 1067, 1158
AsuII TTCGAA 1 cut(s) 1081
AvaII GGWCC 1 cut(s) 1091
BalI TGGCCA 2 cut(s) 362, 872
BanI GGYRCC 3 cut(s) 462, 1010, 1153
BbvI GCAGC 3 cut(s) 593, 665, 793
BccI CCATC 2 cut(s) 208, 899
BceAI ACGGC 2 cut(s) 537, 851
BciT130I CCWGG 2 cut(s) 1051, 1098
BcoDI GTCTC 4 cut(s) 42, 269, 767, 889
BcuI ACTAGT 1 cut(s) 737
BfaI CTAG 2 cut(s) 495, 738
BfmI CTRYAG 1 cut(s) 1172
BisI GCNGC 3 cut(s) 582, 679, 807
BlsI GCNGC 3 cut(s) 583, 680, 808
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 2 cut(s) 1051, 1098
Bme18I GGWCC 1 cut(s) 1091
BmgBI CACGTC 1 cut(s) 162
BmgT120I GGNCC 1 cut(s) 1091
BmiI GGNNCC 4 cut(s) 464, 834, 1012, 1155
BmrFI CCNGG 2 cut(s) 1051, 1098
BmsI GCATC 3 cut(s) 691, 889, 1151
BpmI CTGGAG 2 cut(s) 526, 1080
Bpu14I TTCGAA 1 cut(s) 1081
BpuEI CTTGAG 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 1069
BsaHI GRCGYC 1 cut(s) 262
BsaI GGTCTC 1 cut(s) 42
BsaJI CCNNGG 4 cut(s) 313, 433, 867, 1050
Bsc4I CCNNNNNNNGG 4 cut(s) 535, 760, 1020, 1100
Bse1I ACTGG 3 cut(s) 394, 509, 881
Bse3DI GCAATG 4 cut(s) 90, 147, 215, 1113
BseBI CCWGG 2 cut(s) 1051, 1098
BseDI CCNNGG 4 cut(s) 313, 433, 867, 1050
BseGI GGATG 1 cut(s) 219
BseLI CCNNNNNNNGG 4 cut(s) 535, 760, 1020, 1100
BseMI GCAATG 4 cut(s) 90, 147, 215, 1113
BseMII CTCAG 1 cut(s) 900
BseNI ACTGG 3 cut(s) 394, 509, 881
BseXI GCAGC 3 cut(s) 593, 665, 793
BseYI CCCAGC 1 cut(s) 154
BsgI GTGCAG 1 cut(s) 786
BshFI GGCC 3 cut(s) 362, 866, 872
BshNI GGYRCC 3 cut(s) 462, 1010, 1153
BslFI GGGAC 1 cut(s) 970
BslI CCNNNNNNNGG 4 cut(s) 535, 760, 1020, 1100
BsmAI GTCTC 4 cut(s) 42, 269, 767, 889
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 970
BsnI GGCC 3 cut(s) 362, 866, 872
Bso31I GGTCTC 1 cut(s) 42
Bsp119I TTCGAA 1 cut(s) 1081
Bsp143I GATC 3 cut(s) 178, 661, 853
BspACI CCGC 3 cut(s) 681, 954, 1158
BspANI GGCC 3 cut(s) 362, 866, 872
BspCNI CTCAG 1 cut(s) 899
BspLI GGNNCC 4 cut(s) 464, 834, 1012, 1155
BspPI GGATC 1 cut(s) 173
BspT104I TTCGAA 1 cut(s) 1081
BspT107I GGYRCC 3 cut(s) 462, 1010, 1153
BspTNI GGTCTC 1 cut(s) 42
BsrBI CCGCTC 1 cut(s) 1160
BsrDI GCAATG 4 cut(s) 90, 147, 215, 1113
BsrI ACTGG 3 cut(s) 394, 509, 881
BssECI CCNNGG 4 cut(s) 313, 433, 867, 1050
BssMI GATC 3 cut(s) 178, 661, 853
BssNI GRCGYC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 313
Bst2UI CCWGG 2 cut(s) 1051, 1098
Bst4CI ACNGT 6 cut(s) 222, 239, 630, 653, 821, 1173
Bst6I CTCTTC 1 cut(s) 765
BstACI GRCGYC 1 cut(s) 262
BstBAI YACGTR 1 cut(s) 1069
BstBI TTCGAA 1 cut(s) 1081
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 2 cut(s) 789, 886
BstDSI CCRYGG 2 cut(s) 433, 867
BstENI CCTNNNNNAGG 1 cut(s) 1098
BstF5I GGATG 1 cut(s) 219
BstKTI GATC 3 cut(s) 181, 664, 856
BstMAI GTCTC 4 cut(s) 42, 269, 767, 889
BstMBI GATC 3 cut(s) 178, 661, 853
BstMWI GCNNNNNNNGC 3 cut(s) 572, 581, 899
BstNI CCWGG 2 cut(s) 1051, 1098
BstNSI RCATGY 1 cut(s) 780
BstSCI CCNGG 2 cut(s) 1049, 1096
BstSFI CTRYAG 1 cut(s) 1172
BstV1I GCAGC 3 cut(s) 593, 665, 793
BsuRI GGCC 3 cut(s) 362, 866, 872
BtgI CCRYGG 2 cut(s) 433, 867
BtrI CACGTC 1 cut(s) 162
BtsCI GGATG 1 cut(s) 219
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 4 cut(s) 387, 582, 626, 1169
Cac8I GCNNGC 1 cut(s) 21
Cfr13I GGNCC 1 cut(s) 1091
CseI GACGC 3 cut(s) 237, 251, 328
Csp6I GTAC 3 cut(s) 78, 463, 1123
CviAII CATG 3 cut(s) 777, 971, 1038
CviQI GTAC 3 cut(s) 78, 463, 1123
DdeI CTNAG 2 cut(s) 789, 886
DpnI GATC 3 cut(s) 180, 663, 855
DpnII GATC 3 cut(s) 178, 661, 853
DraI TTTAAA 1 cut(s) 709
EaeI YGGCCR 2 cut(s) 360, 870
Eam1104I CTCTTC 1 cut(s) 765
EarI CTCTTC 1 cut(s) 765
Eco130I CCWWGG 1 cut(s) 313
Eco31I GGTCTC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 1091
Eco57I CTGAAG 2 cut(s) 881, 1023
EcoNI CCTNNNNNAGG 1 cut(s) 1098
EcoRI GAATTC 2 cut(s) 1033, 1074
EcoRII CCWGG 2 cut(s) 1049, 1096
EcoT14I CCWWGG 1 cut(s) 313
ErhI CCWWGG 1 cut(s) 313
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 3 cut(s) 780, 974, 1041
FaqI GGGAC 1 cut(s) 970
FatI CATG 3 cut(s) 776, 970, 1037
FauI CCCGC 2 cut(s) 947, 1165
Fnu4HI GCNGC 3 cut(s) 582, 679, 807
FokI GGATG 1 cut(s) 226
Fsp4HI GCNGC 3 cut(s) 582, 679, 807
FspBI CTAG 2 cut(s) 495, 738
GluI GCNGC 3 cut(s) 582, 679, 807
GsaI CCCAGC 1 cut(s) 158
GsuI CTGGAG 2 cut(s) 526, 1080
HaeIII GGCC 3 cut(s) 362, 866, 872
HgaI GACGC 3 cut(s) 237, 251, 328
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 3 cut(s) 780, 974, 1041
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 1 cut(s) 732
HphI GGTGA 5 cut(s) 251, 259, 403, 1067, 1158
Hpy166II GTNNAC 3 cut(s) 343, 437, 649
Hpy188I TCNGA 8 cut(s) 47, 227, 355, 380, 478, 492, 634, 995
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 3 cut(s) 343, 437, 649
HpyAV CCTTC 4 cut(s) 21, 856, 1001, 1137
HpyCH4III ACNGT 6 cut(s) 222, 239, 630, 653, 821, 1173
HpyCH4IV ACGT 3 cut(s) 161, 442, 1068
HpyCH4V TGCA 4 cut(s) 83, 248, 767, 1142
HpyF10VI GCNNNNNNNGC 3 cut(s) 572, 581, 899
HpyF3I CTNAG 2 cut(s) 789, 886
HpySE526I ACGT 3 cut(s) 161, 442, 1068
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 3 cut(s) 780, 974, 1041
KpnI GGTACC 1 cut(s) 466
Kzo9I GATC 3 cut(s) 178, 661, 853
LmnI GCTCC 2 cut(s) 406, 1165
Lsp1109I GCAGC 3 cut(s) 593, 665, 793
LweI GCATC 3 cut(s) 691, 889, 1151
MaeI CTAG 2 cut(s) 495, 738
MaeII ACGT 3 cut(s) 161, 442, 1068
MaeIII GTNAC 7 cut(s) 239, 409, 523, 611, 653, 779, 976
MalI GATC 3 cut(s) 180, 663, 855
MbiI CCGCTC 1 cut(s) 1160
MboI GATC 3 cut(s) 178, 661, 853
MboII GAAGA 8 cut(s) 292, 380, 710, 755, 782, 1016, 1042, 1070
MfeI CAATTG 1 cut(s) 617
MlsI TGGCCA 2 cut(s) 362, 872
MluCI AATT 9 cut(s) 111, 371, 498, 617, 666, 914, 949, 1033, 1074
MluNI TGGCCA 2 cut(s) 362, 872
MmeI TCCRAC 3 cut(s) 189, 515, 1160
MnlI CCTC 9 cut(s) 64, 193, 501, 583, 722, 846, 1094, 1104, 1174
Mox20I TGGCCA 2 cut(s) 362, 872
MroXI GAANNNNTTC 2 cut(s) 372, 831
MscI TGGCCA 2 cut(s) 362, 872
MseI TTAA 4 cut(s) 335, 416, 669, 708
MslI CAYNNNNRTG 2 cut(s) 275, 1118
Msp20I TGGCCA 2 cut(s) 362, 872
MspR9I CCNGG 2 cut(s) 1051, 1098
MunI CAATTG 1 cut(s) 617
MvaI CCWGG 2 cut(s) 1051, 1098
MwoI GCNNNNNNNGC 3 cut(s) 572, 581, 899
NdeII GATC 3 cut(s) 178, 661, 853
NlaIII CATG 3 cut(s) 780, 974, 1041
NlaIV GGNNCC 4 cut(s) 464, 834, 1012, 1155
NmuCI GTSAC 3 cut(s) 239, 409, 779
NspI RCATGY 1 cut(s) 780
NspV TTCGAA 1 cut(s) 1081
PciI ACATGT 1 cut(s) 776
PdmI GAANNNNTTC 2 cut(s) 372, 831
PfeI GAWTC 1 cut(s) 732
PflMI CCANNNNNTGG 1 cut(s) 1020
PfoI TCCNGGA 1 cut(s) 1096
PkrI GCNGC 3 cut(s) 583, 680, 808
Ppu21I YACGTR 1 cut(s) 1069
PscI ACATGT 1 cut(s) 776
PsiI TTATAA 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 1049, 1096
PspFI CCCAGC 1 cut(s) 154
PspGI CCWGG 2 cut(s) 1049, 1096
PspN4I GGNNCC 4 cut(s) 464, 834, 1012, 1155
PspPI GGNCC 1 cut(s) 1091
RsaI GTAC 3 cut(s) 79, 464, 1124
RsaNI GTAC 3 cut(s) 78, 463, 1123
RseI CAYNNNNRTG 2 cut(s) 275, 1118
SaqAI TTAA 4 cut(s) 335, 416, 669, 708
SatI GCNGC 3 cut(s) 582, 679, 807
Sau3AI GATC 3 cut(s) 178, 661, 853
Sau96I GGNCC 1 cut(s) 1091
ScaI AGTACT 1 cut(s) 79
ScrFI CCNGG 2 cut(s) 1051, 1098
SfaNI GCATC 3 cut(s) 691, 889, 1151
SfcI CTRYAG 1 cut(s) 1172
SfuI TTCGAA 1 cut(s) 1081
SinI GGWCC 1 cut(s) 1091
SmiMI CAYNNNNRTG 2 cut(s) 275, 1118
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
SpeI ACTAGT 1 cut(s) 737
Sse9I AATT 9 cut(s) 111, 371, 498, 617, 666, 914, 949, 1033, 1074
SsiI CCGC 3 cut(s) 681, 954, 1158
SspMI CTAG 2 cut(s) 495, 738
StyD4I CCNGG 2 cut(s) 1049, 1096
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 6 cut(s) 222, 239, 630, 653, 821, 1173
TaiI ACGT 3 cut(s) 164, 445, 1071
TaqI TCGA 1 cut(s) 1081
TasI AATT 9 cut(s) 111, 371, 498, 617, 666, 914, 949, 1033, 1074
TatI WGTACW 1 cut(s) 77
TfiI GAWTC 1 cut(s) 732
Tru1I TTAA 4 cut(s) 335, 416, 669, 708
Tru9I TTAA 4 cut(s) 335, 416, 669, 708
TscAI CASTG 4 cut(s) 394, 582, 633, 1176
TseFI GTSAC 3 cut(s) 239, 409, 779
TseI GCWGC 3 cut(s) 581, 678, 806
Tsp45I GTSAC 3 cut(s) 239, 409, 779
TspDTI ATGAA 5 cut(s) 186, 381, 840, 927, 1026
TspGWI ACGGA 2 cut(s) 422, 741
TspRI CASTG 4 cut(s) 394, 582, 633, 1176
Van91I CCANNNNNTGG 1 cut(s) 1020
VpaK11BI GGWCC 1 cut(s) 1091
XagI CCTNNNNNAGG 1 cut(s) 1098
XapI RAATTY 4 cut(s) 371, 914, 1033, 1074
XceI RCATGY 1 cut(s) 780
XmnI GAANNNNTTC 2 cut(s) 372, 831
XspI CTAG 2 cut(s) 495, 738
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.