Rh3AG064200

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0008144
Physical Location & Seq
Forward (+)
4428743 .. 4429872
1130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG064200.1

Sequence Viewer

Length: 1035 bp
ATGGCACAACCCCCAGCCACGTCCAACGATGAAGTTGATCCCTCTACATTTTTGAAAACATTTGAGCAATGGATGGCACAGTTCGGACGCGCTTACAGTGACAATGCAGAGAAAGAAAGGCGTCTCACCATATTCGTGAAGAACTTGTTATTTGTAAATAACTTCAACAGCCAAGGGAATAAGACTTACAAGTTAAGCGTCAACTTGTTTTCTGATATGAGCACTGAAGAATTTCTCGGACTTTACACTGGATTTCAAGCTCCCAACGTCACCTTAAACTCAAGCTCATTCCGTGGACAACGTAATATCAAATCTTTTAGGTACCAAGACCTGTCTGAAACTGATGTTCCGACTAGAATTGACTGGAGGGAACAAGGTGCTGTTACCCCCGTCAAGGAGCAAGGACAATGCAGTTCCTGTTGGGCATTTTCAGCAGTGGCAACAATTGAGGGCATTACCAAAATCAAAAGTAACCAATTGATTTCACTGTCCGAACAACAACTTGTGGACTGTAACAGAGATCAAATTAACAAGGGCTGCGGTGGTGGTAATATGGAAGATGCCTTTAAATACATAAATGACAACGGAGGAATCACTAGTGAAGAAAACTACCAATACAAGGGTGCAGAAGAGACATGTGACACTACTAAGACGACTGAATATGCTGCCAAGATAAACGGTTATGAAATGGTTCCTCCCAATAGCGAAAATGATCTACTGAAGGCCGTGGCCAAGCAACCAGTCTCAGCTGCCCTTGATGCCGATGGAGATGAATTTAGGGCATACGCAAGTGGGGTATTTTCTGGTAATTGCGGGACAAACCTAACCCATGCTGTTACAATAGTTGGGTACGGAACGACTGAAGAAGGCACCAACTATTGGTTAGTGAAGAATTCATGGGGCGAAACCTGGGGTGAAAAGGGCTACGTGAGAATTCTTCGAAATGCTGGTCCTCCAGGAGGTTTGTGTGGCATTGCTATGTACCCTTCTTATCCAACTGCATCAGCTATGGCACCCGCTCCCTCACCACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

344

Amino Acids

37.79

Weight (kDa)

4.85

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 21 - 78 2e-18 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 116 - 333 3.3e-80 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 321
AccB1I GGYRCC 3 cut(s) 321, 869, 1012
AccB7I CCANNNNNTGG 1 cut(s) 879
AccBSI CCGCTC 1 cut(s) 1019
AccII CGCG 1 cut(s) 90
AciI CCGC 3 cut(s) 540, 813, 1017
AclWI GGATC 1 cut(s) 32
AcoI YGGCCR 1 cut(s) 729
AcsI RAATTY 4 cut(s) 230, 773, 892, 933
AcuI CTGAAG 3 cut(s) 246, 740, 882
AcyI GRCGYC 1 cut(s) 121
AfaI GTAC 3 cut(s) 323, 851, 983
AfiI CCNNNNNNNGG 4 cut(s) 394, 619, 879, 959
AflIII ACRYGT 1 cut(s) 635
AgsI TTSAA 3 cut(s) 55, 166, 257
AhlI ACTAGT 1 cut(s) 596
AjiI CACGTC 1 cut(s) 21
AjnI CCWGG 2 cut(s) 908, 955
AluBI AGCT 4 cut(s) 260, 285, 749, 1007
AluI AGCT 4 cut(s) 260, 285, 749, 1007
Alw21I GWGCWC 1 cut(s) 224
Alw26I GTCTC 3 cut(s) 128, 626, 748
AlwI GGATC 1 cut(s) 32
AlwNI CAGNNNCTG 1 cut(s) 417
AoxI GGCC 2 cut(s) 723, 729
ApeKI GCWGC 3 cut(s) 537, 665, 749
ApoI RAATTY 4 cut(s) 230, 773, 892, 933
ArsI GACNNNNNNTTYG 2 cut(s) 934, 966
Asp700I GAANNNNTTC 2 cut(s) 231, 690
Asp718I GGTACC 1 cut(s) 321
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 950
AsuHPI GGTGA 4 cut(s) 118, 262, 926, 1017
AsuII TTCGAA 1 cut(s) 940
AvaII GGWCC 1 cut(s) 950
BalI TGGCCA 1 cut(s) 731
BanI GGYRCC 3 cut(s) 321, 869, 1012
Bbv12I GWGCWC 1 cut(s) 224
BbvI GCAGC 3 cut(s) 524, 652, 736
BccI CCATC 2 cut(s) 67, 758
BceAI ACGGC 1 cut(s) 710
BciT130I CCWGG 2 cut(s) 910, 957
BcoDI GTCTC 3 cut(s) 128, 626, 748
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 2 cut(s) 354, 597
BfmI CTRYAG 1 cut(s) 1031
BisI GCNGC 3 cut(s) 538, 666, 750
BlsI GCNGC 3 cut(s) 539, 667, 751
Bme1390I CCNGG 2 cut(s) 910, 957
Bme18I GGWCC 1 cut(s) 950
BmgBI CACGTC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 950
BmiI GGNNCC 4 cut(s) 323, 693, 871, 1014
BmrFI CCNGG 2 cut(s) 910, 957
BmsI GCATC 3 cut(s) 550, 748, 1010
BpmI CTGGAG 2 cut(s) 385, 939
Bpu14I TTCGAA 1 cut(s) 940
BpuEI CTTGAG 1 cut(s) 265
BsaAI YACGTR 1 cut(s) 928
BsaHI GRCGYC 1 cut(s) 121
BsaJI CCNNGG 4 cut(s) 172, 292, 726, 909
Bsc4I CCNNNNNNNGG 4 cut(s) 394, 619, 879, 959
Bse1I ACTGG 3 cut(s) 253, 368, 740
Bse3DI GCAATG 2 cut(s) 74, 972
BseBI CCWGG 2 cut(s) 910, 957
BseDI CCNNGG 4 cut(s) 172, 292, 726, 909
BseGI GGATG 1 cut(s) 78
BseLI CCNNNNNNNGG 4 cut(s) 394, 619, 879, 959
BseMI GCAATG 2 cut(s) 74, 972
BseMII CTCAG 1 cut(s) 759
BseNI ACTGG 3 cut(s) 253, 368, 740
BseXI GCAGC 3 cut(s) 524, 652, 736
BseYI CCCAGC 1 cut(s) 13
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 1 cut(s) 90
BshFI GGCC 2 cut(s) 725, 731
BshNI GGYRCC 3 cut(s) 321, 869, 1012
BsiHKAI GWGCWC 1 cut(s) 224
BslFI GGGAC 1 cut(s) 829
BslI CCNNNNNNNGG 4 cut(s) 394, 619, 879, 959
BsmAI GTCTC 3 cut(s) 128, 626, 748
BsmBI CGTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 829
BsnI GGCC 2 cut(s) 725, 731
Bsp119I TTCGAA 1 cut(s) 940
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 3 cut(s) 37, 520, 712
BspACI CCGC 3 cut(s) 540, 813, 1017
BspANI GGCC 2 cut(s) 725, 731
BspCNI CTCAG 1 cut(s) 758
BspFNI CGCG 1 cut(s) 90
BspLI GGNNCC 4 cut(s) 323, 693, 871, 1014
BspPI GGATC 1 cut(s) 32
BspT104I TTCGAA 1 cut(s) 940
BspT107I GGYRCC 3 cut(s) 321, 869, 1012
BsrBI CCGCTC 1 cut(s) 1019
BsrDI GCAATG 2 cut(s) 74, 972
BsrI ACTGG 3 cut(s) 253, 368, 740
BssECI CCNNGG 4 cut(s) 172, 292, 726, 909
BssMI GATC 3 cut(s) 37, 520, 712
BssNI GRCGYC 1 cut(s) 121
BssT1I CCWWGG 1 cut(s) 172
Bst2UI CCWGG 2 cut(s) 910, 957
Bst4CI ACNGT 6 cut(s) 81, 98, 489, 512, 680, 1032
Bst6I CTCTTC 1 cut(s) 624
BstACI GRCGYC 1 cut(s) 121
BstBAI YACGTR 1 cut(s) 928
BstBI TTCGAA 1 cut(s) 940
BstDEI CTNAG 2 cut(s) 648, 745
BstDSI CCRYGG 2 cut(s) 292, 726
BstENI CCTNNNNNAGG 1 cut(s) 957
BstF5I GGATG 1 cut(s) 78
BstFNI CGCG 1 cut(s) 90
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 3 cut(s) 40, 523, 715
BstMAI GTCTC 3 cut(s) 128, 626, 748
BstMBI GATC 3 cut(s) 37, 520, 712
BstMWI GCNNNNNNNGC 2 cut(s) 431, 758
BstNI CCWGG 2 cut(s) 910, 957
BstNSI RCATGY 1 cut(s) 639
BstSCI CCNGG 2 cut(s) 908, 955
BstSFI CTRYAG 1 cut(s) 1031
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 524, 652, 736
BsuRI GGCC 2 cut(s) 725, 731
BtgI CCRYGG 2 cut(s) 292, 726
BtrI CACGTC 1 cut(s) 21
BtsCI GGATG 1 cut(s) 78
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 6 cut(s) 103, 222, 246, 441, 485, 1028
CaiI CAGNNNCTG 1 cut(s) 417
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 950
CseI GACGC 3 cut(s) 96, 110, 187
Csp6I GTAC 3 cut(s) 322, 850, 982
CviAII CATG 3 cut(s) 636, 830, 897
CviQI GTAC 3 cut(s) 322, 850, 982
DdeI CTNAG 2 cut(s) 648, 745
DpnI GATC 3 cut(s) 39, 522, 714
DpnII GATC 3 cut(s) 37, 520, 712
DraI TTTAAA 1 cut(s) 568
EaeI YGGCCR 1 cut(s) 729
Eam1104I CTCTTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 624
Eco130I CCWWGG 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 950
Eco57I CTGAAG 3 cut(s) 246, 740, 882
EcoNI CCTNNNNNAGG 1 cut(s) 957
EcoRI GAATTC 2 cut(s) 892, 933
EcoRII CCWGG 2 cut(s) 908, 955
EcoT14I CCWWGG 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 172
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 3 cut(s) 639, 833, 900
FaqI GGGAC 1 cut(s) 829
FatI CATG 3 cut(s) 635, 829, 896
FauI CCCGC 2 cut(s) 806, 1024
Fnu4HI GCNGC 3 cut(s) 538, 666, 750
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 3 cut(s) 538, 666, 750
FspBI CTAG 2 cut(s) 354, 597
GlaI GCGC 1 cut(s) 91
GluI GCNGC 3 cut(s) 538, 666, 750
GsaI CCCAGC 1 cut(s) 17
GsuI CTGGAG 2 cut(s) 385, 939
HaeIII GGCC 2 cut(s) 725, 731
HgaI GACGC 3 cut(s) 96, 110, 187
HhaI GCGC 1 cut(s) 92
Hin1I GRCGYC 1 cut(s) 121
Hin1II CATG 3 cut(s) 639, 833, 900
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 1 cut(s) 591
HphI GGTGA 4 cut(s) 118, 262, 926, 1017
Hpy166II GTNNAC 3 cut(s) 202, 296, 508
Hpy188I TCNGA 6 cut(s) 86, 214, 239, 337, 351, 493
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 3 cut(s) 202, 296, 508
HpyAV CCTTC 3 cut(s) 715, 860, 996
HpyCH4III ACNGT 6 cut(s) 81, 98, 489, 512, 680, 1032
HpyCH4IV ACGT 4 cut(s) 20, 267, 301, 927
HpyCH4V TGCA 4 cut(s) 107, 411, 626, 1001
HpyF10VI GCNNNNNNNGC 2 cut(s) 431, 758
HpyF3I CTNAG 2 cut(s) 648, 745
HpySE526I ACGT 4 cut(s) 20, 267, 301, 927
Hsp92I GRCGYC 1 cut(s) 121
Hsp92II CATG 3 cut(s) 639, 833, 900
HspAI GCGC 1 cut(s) 90
KpnI GGTACC 1 cut(s) 325
Kzo9I GATC 3 cut(s) 37, 520, 712
LmnI GCTCC 3 cut(s) 265, 397, 1024
Lsp1109I GCAGC 3 cut(s) 524, 652, 736
LweI GCATC 3 cut(s) 550, 748, 1010
MaeI CTAG 2 cut(s) 354, 597
MaeII ACGT 4 cut(s) 20, 267, 301, 927
MaeIII GTNAC 7 cut(s) 98, 268, 382, 470, 512, 638, 835
MalI GATC 3 cut(s) 39, 522, 714
MbiI CCGCTC 1 cut(s) 1019
MboI GATC 3 cut(s) 37, 520, 712
MboII GAAGA 8 cut(s) 151, 239, 569, 614, 641, 875, 901, 929
MfeI CAATTG 2 cut(s) 444, 476
MhlI GDGCHC 1 cut(s) 224
MlsI TGGCCA 1 cut(s) 731
MluCI AATT 9 cut(s) 230, 357, 444, 476, 525, 773, 808, 892, 933
MluNI TGGCCA 1 cut(s) 731
MmeI TCCRAC 3 cut(s) 48, 374, 1019
MnlI CCTC 8 cut(s) 52, 360, 442, 581, 705, 953, 963, 1033
Mox20I TGGCCA 1 cut(s) 731
MroXI GAANNNNTTC 2 cut(s) 231, 690
MscI TGGCCA 1 cut(s) 731
MseI TTAA 4 cut(s) 194, 275, 528, 567
MslI CAYNNNNRTG 2 cut(s) 134, 977
Msp20I TGGCCA 1 cut(s) 731
MspA1I CMGCKG 1 cut(s) 749
MspR9I CCNGG 2 cut(s) 910, 957
MunI CAATTG 2 cut(s) 444, 476
MvaI CCWGG 2 cut(s) 910, 957
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 2 cut(s) 431, 758
NdeII GATC 3 cut(s) 37, 520, 712
NlaIII CATG 3 cut(s) 639, 833, 900
NlaIV GGNNCC 4 cut(s) 323, 693, 871, 1014
NmuCI GTSAC 3 cut(s) 98, 268, 638
NspI RCATGY 1 cut(s) 639
NspV TTCGAA 1 cut(s) 940
PciI ACATGT 1 cut(s) 635
PdmI GAANNNNTTC 2 cut(s) 231, 690
PfeI GAWTC 1 cut(s) 591
PflMI CCANNNNNTGG 1 cut(s) 879
PfoI TCCNGGA 1 cut(s) 955
PkrI GCNGC 3 cut(s) 539, 667, 751
Ppu21I YACGTR 1 cut(s) 928
PscI ACATGT 1 cut(s) 635
Psp6I CCWGG 2 cut(s) 908, 955
PspFI CCCAGC 1 cut(s) 13
PspGI CCWGG 2 cut(s) 908, 955
PspN4I GGNNCC 4 cut(s) 323, 693, 871, 1014
PspPI GGNCC 1 cut(s) 950
PstNI CAGNNNCTG 1 cut(s) 417
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 3 cut(s) 323, 851, 983
RsaNI GTAC 3 cut(s) 322, 850, 982
RseI CAYNNNNRTG 2 cut(s) 134, 977
SaqAI TTAA 4 cut(s) 194, 275, 528, 567
SatI GCNGC 3 cut(s) 538, 666, 750
Sau3AI GATC 3 cut(s) 37, 520, 712
Sau96I GGNCC 1 cut(s) 950
ScrFI CCNGG 2 cut(s) 910, 957
SduI GDGCHC 1 cut(s) 224
SfaNI GCATC 3 cut(s) 550, 748, 1010
SfcI CTRYAG 1 cut(s) 1031
SfuI TTCGAA 1 cut(s) 940
SinI GGWCC 1 cut(s) 950
SmiMI CAYNNNNRTG 2 cut(s) 134, 977
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
SpeI ACTAGT 1 cut(s) 596
Sse9I AATT 9 cut(s) 230, 357, 444, 476, 525, 773, 808, 892, 933
SsiI CCGC 3 cut(s) 540, 813, 1017
SspMI CTAG 2 cut(s) 354, 597
StyD4I CCNGG 2 cut(s) 908, 955
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 6 cut(s) 81, 98, 489, 512, 680, 1032
TaiI ACGT 4 cut(s) 23, 270, 304, 930
TaqI TCGA 1 cut(s) 940
TasI AATT 9 cut(s) 230, 357, 444, 476, 525, 773, 808, 892, 933
TfiI GAWTC 1 cut(s) 591
Tru1I TTAA 4 cut(s) 194, 275, 528, 567
Tru9I TTAA 4 cut(s) 194, 275, 528, 567
TscAI CASTG 6 cut(s) 103, 229, 253, 441, 492, 1035
TseFI GTSAC 3 cut(s) 98, 268, 638
TseI GCWGC 3 cut(s) 537, 665, 749
Tsp45I GTSAC 3 cut(s) 98, 268, 638
TspDTI ATGAA 4 cut(s) 45, 699, 786, 885
TspGWI ACGGA 3 cut(s) 281, 600, 867
TspRI CASTG 6 cut(s) 103, 229, 253, 441, 492, 1035
Van91I CCANNNNNTGG 1 cut(s) 879
VpaK11BI GGWCC 1 cut(s) 950
XagI CCTNNNNNAGG 1 cut(s) 957
XapI RAATTY 4 cut(s) 230, 773, 892, 933
XceI RCATGY 1 cut(s) 639
XmnI GAANNNNTTC 2 cut(s) 231, 690
XspI CTAG 2 cut(s) 354, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.