Rh3BG065600

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008144
Physical Location & Seq
Forward (+)
4381164 .. 4390934
9771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG065600.1

Sequence Viewer

Length: 774 bp
ATGGCCCGACACCCTTGTGGTGGATGGCGCTCCACCACATTTGGCAACTACATCATTGGTGGCACCCTTGCTAAATCAAAGTCATTTAGTATCAGAGAGCCCTCATTGTTATATACTGGGTGTAGTACTGCAATGGGTTTTCAAAATAACCTTGTTATAGTTGCCATTTTATCTATCATCTTGGGGGCAATGGCACAACCCCCAGCCACGACCAACGATGAATTTGATCCCTCTACATTTTTTAAAACATTTGAGGAATGGATGGCACAGTTCGGACGCACTTACGATGACAATGCAGAGAGAGAAAGGCGTCTAACCATATTCGTGAAGAACTTGTTATTTGTAAATAACTTCAACAGCCAAGGGAATAAGACTTTCAAGTTAAGAGTCAACTTATTTTCTGATATGTCCAATGAAGAATTTCTCGGACTTTACACTGGATTTCAAGCTCCCGAGGTCACCTTAAACTCAAGCTCATTCCGAGGACAACGTAATATGAAATCTTTTAGGTACCAAGACCTGTCTGAAACTGATGTTCCAACTAGAATTGACTGGAGGGAACAAGGTGTTGTTACCCCCGTCAAGGAACAAGGACAATGCAGTAAGAACCACCATATCTCAAACCTTGATCAATTCCTTTTGAAAGCTTCTGAAGCAATTGAATTGAAGTTGTCGCCATTGGCTGCGGCTCAGAGTCAAGCTATGGCTCTGAGGCGAGCAGCGGAAGTCGTCACTCTTGGCTATACAGATAGGGATCACTATCTTCATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

257

Amino Acids

29.04

Weight (kDa)

6.97

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 84 - 141 1.4e-18 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 510
AccB1I GGYRCC 2 cut(s) 62, 510
AciI CCGC 2 cut(s) 686, 722
AclWI GGATC 2 cut(s) 221, 762
AcsI RAATTY 2 cut(s) 221, 419
AcuI CTGAAG 1 cut(s) 672
AcyI GRCGYC 1 cut(s) 310
AfaI GTAC 2 cut(s) 127, 512
AfiI CCNNNNNNNGG 2 cut(s) 20, 583
AgsI TTSAA 7 cut(s) 143, 355, 379, 446, 643, 662, 667
AleI CACNNNNGTG 1 cut(s) 15
AluBI AGCT 4 cut(s) 449, 474, 647, 701
AluI AGCT 4 cut(s) 449, 474, 647, 701
AlwI GGATC 2 cut(s) 221, 762
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 683, 719
ApoI RAATTY 2 cut(s) 221, 419
Asp700I GAANNNNTTC 1 cut(s) 420
Asp718I GGTACC 1 cut(s) 510
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 451
AvaI CYCGRG 1 cut(s) 452
BanI GGYRCC 2 cut(s) 62, 510
BanII GRGCYC 1 cut(s) 102
BbvI GCAGC 2 cut(s) 670, 731
BccI CCATC 2 cut(s) 18, 256
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BclI TGATCA 1 cut(s) 628
BfaI CTAG 1 cut(s) 543
BfoI RGCGCY 1 cut(s) 31
BisI GCNGC 3 cut(s) 684, 687, 720
BlsI GCNGC 3 cut(s) 685, 688, 721
BmcAI AGTACT 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 64, 512
BmrI ACTGGG 1 cut(s) 126
BmuI ACTGGG 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 574
BpuEI CTTGAG 1 cut(s) 454
BsaBI GATNNNNATC 2 cut(s) 753, 759
BsaHI GRCGYC 1 cut(s) 310
BsaJI CCNNGG 3 cut(s) 361, 453, 481
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 583
Bse1I ACTGG 3 cut(s) 121, 442, 557
Bse3DI GCAATG 2 cut(s) 138, 195
Bse8I GATNNNNATC 2 cut(s) 753, 759
BseDI CCNNGG 3 cut(s) 361, 453, 481
BseGI GGATG 2 cut(s) 29, 267
BseJI GATNNNNATC 2 cut(s) 753, 759
BseLI CCNNNNNNNGG 2 cut(s) 20, 583
BseMI GCAATG 2 cut(s) 138, 195
BseMII CTCAG 2 cut(s) 701, 704
BseNI ACTGG 3 cut(s) 121, 442, 557
BseXI GCAGC 2 cut(s) 670, 731
BseYI CCCAGC 1 cut(s) 202
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 62, 510
BsiHKCI CYCGRG 1 cut(s) 452
BslI CCNNNNNNNGG 2 cut(s) 20, 583
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 452
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 3 cut(s) 226, 628, 754
BspACI CCGC 2 cut(s) 686, 722
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 702, 703
BspHI TCATGA 1 cut(s) 766
BspLI GGNNCC 2 cut(s) 64, 512
BspPI GGATC 2 cut(s) 221, 762
BspT107I GGYRCC 2 cut(s) 62, 510
BsrDI GCAATG 2 cut(s) 138, 195
BsrI ACTGG 3 cut(s) 121, 442, 557
BssECI CCNNGG 3 cut(s) 361, 453, 481
BssMI GATC 3 cut(s) 226, 628, 754
BssNI GRCGYC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 361
Bst4CI ACNGT 1 cut(s) 270
BstACI GRCGYC 1 cut(s) 310
BstC8I GCNNGC 1 cut(s) 717
BstDEI CTNAG 2 cut(s) 690, 710
BstEII GGTNACC 1 cut(s) 457
BstF5I GGATG 2 cut(s) 29, 267
BstH2I RGCGCY 1 cut(s) 31
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 3 cut(s) 229, 631, 757
BstMBI GATC 3 cut(s) 226, 628, 754
BstMWI GCNNNNNNNGC 1 cut(s) 653
BstPI GGTNACC 1 cut(s) 457
BstV1I GCAGC 2 cut(s) 670, 731
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 29, 267
BtsIMutI CAGTG 1 cut(s) 435
Cac8I GCNNGC 1 cut(s) 717
CciI TCATGA 1 cut(s) 766
CfoI GCGC 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 2 cut(s) 285, 299
Csp6I GTAC 2 cut(s) 126, 511
CspCI CAANNNNNGTGG 2 cut(s) 22, 57
CviAII CATG 1 cut(s) 767
CviQI GTAC 2 cut(s) 126, 511
DdeI CTNAG 2 cut(s) 690, 710
DpnI GATC 3 cut(s) 228, 630, 756
DpnII GATC 3 cut(s) 226, 628, 754
DraI TTTAAA 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 452
Eco91I GGTNACC 1 cut(s) 457
EcoO65I GGTNACC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 361
FaeI CATG 1 cut(s) 770
FatI CATG 1 cut(s) 766
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 3 cut(s) 684, 687, 720
FokI GGATG 2 cut(s) 36, 274
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 3 cut(s) 684, 687, 720
FspBI CTAG 1 cut(s) 543
GlaI GCGC 1 cut(s) 29
GluI GCNGC 3 cut(s) 684, 687, 720
GsaI CCCAGC 1 cut(s) 206
GsuI CTGGAG 1 cut(s) 574
HaeII RGCGCY 1 cut(s) 31
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 2 cut(s) 285, 299
HhaI GCGC 1 cut(s) 30
Hin1I GRCGYC 1 cut(s) 310
Hin1II CATG 1 cut(s) 770
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HindIII AAGCTT 1 cut(s) 645
HinfI GANTC 2 cut(s) 387, 694
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 9 cut(s) 95, 275, 403, 428, 482, 526, 652, 693, 711
Hpy188III TCNNGA 3 cut(s) 325, 452, 767
Hpy8I GTNNAC 1 cut(s) 391
HpyCH4III ACNGT 1 cut(s) 270
HpyCH4IV ACGT 1 cut(s) 490
HpyCH4V TGCA 3 cut(s) 131, 296, 600
HpyF10VI GCNNNNNNNGC 1 cut(s) 653
HpyF3I CTNAG 2 cut(s) 690, 710
HpySE526I ACGT 1 cut(s) 490
Hsp92I GRCGYC 1 cut(s) 310
Hsp92II CATG 1 cut(s) 770
HspAI GCGC 1 cut(s) 28
KpnI GGTACC 1 cut(s) 514
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 3 cut(s) 226, 628, 754
LmnI GCTCC 2 cut(s) 35, 454
LpnPI CCDG 5 cut(s) 102, 216, 423, 533, 538
Lsp1109I GCAGC 2 cut(s) 670, 731
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 490
MaeIII GTNAC 3 cut(s) 457, 571, 730
MalI GATC 3 cut(s) 228, 630, 756
MboI GATC 3 cut(s) 226, 628, 754
MboII GAAGA 3 cut(s) 340, 428, 755
MfeI CAATTG 1 cut(s) 657
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 6 cut(s) 221, 419, 546, 632, 657, 662
MlyI GAGTC 2 cut(s) 396, 703
MmeI TCCRAC 1 cut(s) 563
MnlI CCTC 7 cut(s) 112, 241, 247, 448, 476, 549, 705
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 3 cut(s) 243, 383, 464
MslI CAYNNNNRTG 2 cut(s) 15, 323
MspA1I CMGCKG 1 cut(s) 722
MunI CAATTG 1 cut(s) 657
MwoI GCNNNNNNNGC 1 cut(s) 653
NdeII GATC 3 cut(s) 226, 628, 754
NlaIII CATG 1 cut(s) 770
NlaIV GGNNCC 2 cut(s) 64, 512
NmuCI GTSAC 2 cut(s) 457, 730
OliI CACNNNNGTG 1 cut(s) 15
PagI TCATGA 1 cut(s) 766
PdmI GAANNNNTTC 1 cut(s) 420
PkrI GCNGC 3 cut(s) 685, 688, 721
PleI GAGTC 2 cut(s) 395, 702
PpsI GAGTC 2 cut(s) 395, 702
PspEI GGTNACC 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 202
PspN4I GGNNCC 2 cut(s) 64, 512
PspPI GGNCC 1 cut(s) 4
RsaI GTAC 2 cut(s) 127, 512
RsaNI GTAC 2 cut(s) 126, 511
RseI CAYNNNNRTG 2 cut(s) 15, 323
SaqAI TTAA 3 cut(s) 243, 383, 464
SatI GCNGC 3 cut(s) 684, 687, 720
Sau3AI GATC 3 cut(s) 226, 628, 754
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 127
SchI GAGTC 2 cut(s) 396, 703
SduI GDGCHC 1 cut(s) 102
SmiMI CAYNNNNRTG 2 cut(s) 15, 323
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
Sse9I AATT 6 cut(s) 221, 419, 546, 632, 657, 662
SsiI CCGC 2 cut(s) 686, 722
SspMI CTAG 1 cut(s) 543
StyI CCWWGG 1 cut(s) 361
TaaI ACNGT 1 cut(s) 270
TaiI ACGT 1 cut(s) 493
TasI AATT 6 cut(s) 221, 419, 546, 632, 657, 662
TatI WGTACW 1 cut(s) 125
TauI GCSGC 1 cut(s) 689
Tru1I TTAA 3 cut(s) 243, 383, 464
Tru9I TTAA 3 cut(s) 243, 383, 464
TscAI CASTG 1 cut(s) 442
TseFI GTSAC 2 cut(s) 457, 730
TseI GCWGC 2 cut(s) 683, 719
Tsp45I GTSAC 2 cut(s) 457, 730
TspDTI ATGAA 4 cut(s) 234, 429, 512, 755
TspRI CASTG 1 cut(s) 442
XapI RAATTY 2 cut(s) 221, 419
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 1 cut(s) 543
ZrmI AGTACT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.