Rw3G005030

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0008144
Physical Location & Seq
Forward (+)
4196823 .. 4198011
1189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G005030.1

Sequence Viewer

Length: 1092 bp
ATGGCTTTTCAAAATAACCTTGTTATAATTGCCATTTTATCTATCATCTTGGGGGCAACGGCACAACCTCCAGCCACGTCCAACGATGAAGTTGATCCCTCGACATTTTTGAAAACATTTGAGCAATGGATGGCACAGTTCGGACGCACTTACGGTGACAATGCAGAGAAAGAAAGGCGTCTCACCATATTCGTGAAGAACTTGTTATTTGTAAATAACTTCAACAGCCAAGGGAATAAGACTTACAAGTTAAGCGTCAACTTGTTTTCTGATATGGCCAATGAAGAATTTCTCGGACTTTACACTGGATTTCAAGCTCCCAATGTCACCTTAAACTCAAGCTCATTCCGTGGACAACGTAATATCAAATCTTTTAGGTACCAAGACCTGTCTGAAACTGATGTTCCGACTAGAATTGACTGGAGGGAACAAGGTGCTGTTACCCCCGTCAAGGAACAAGGACAATGCAGTTCTTGTTGGGCATTTTCAGCAGTGGCAGCAATTGAGGGCATTACCAAAATCAAAAGTAACCAATTGATTTCACTGTCCGAGCAACAACTTGTGGACTGTAACAGAGATCAAATTAACAAGGGCTGCGGTGGTGGTAATATGGAAGATGCCTTTAAATACATAAATGACAACGGAGGAATCACTAGTGAAGAAAACTACCAATACAAGGGTGCAGAAGAGACATGTGACACTACTAAGACGACTGAATATGCTGCCAAGATAAACGGTTATGAAATGGTTCCTCCCAACAGCGAAAATGATCTACTAAAGGCCGTGGCCAAGCAACCAGTCTCAGTCGCCCTTGATGCCGATGGAGCTGAATTTAGGGCATACGCAAGTGGGGTATTTTCTGGTAATTGCGGGACCAACCTAACCCATGCTGTTACAATCGTTGGGTACGGAACGACTAAAGAAGGCACCAACTATTGGTTAGTGAAGAATTCATGGGGCGAAACCTGGGGTGAAAAGGGCTACATGAGAATTCTTCGAAATGCTGGTCCTCCAGGAGGTTTGTGTGGCATTGCTATGTACCCTTCTTATCCAACTGCATCAGCTATGGCACCCGCTCCCTCACCACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

363

Amino Acids

39.74

Weight (kDa)

5.04

Isoelectric Point (pI)

34.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 40 - 97 4.2e-18 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 135 - 352 6.3e-81 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
Acc65I GGTACC 1 cut(s) 378
AccB1I GGYRCC 3 cut(s) 378, 926, 1069
AccB7I CCANNNNNTGG 1 cut(s) 936
AccBSI CCGCTC 1 cut(s) 1076
AciI CCGC 3 cut(s) 597, 870, 1074
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 2 cut(s) 276, 786
AcsI RAATTY 4 cut(s) 287, 830, 949, 990
AcyI GRCGYC 1 cut(s) 178
AfaI GTAC 3 cut(s) 380, 908, 1040
AfiI CCNNNNNNNGG 4 cut(s) 451, 676, 936, 1016
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 4 cut(s) 11, 112, 223, 314
AhlI ACTAGT 1 cut(s) 653
AjiI CACGTC 1 cut(s) 78
AjnI CCWGG 2 cut(s) 965, 1012
AluBI AGCT 4 cut(s) 317, 342, 827, 1064
AluI AGCT 4 cut(s) 317, 342, 827, 1064
Alw26I GTCTC 3 cut(s) 185, 683, 805
AlwI GGATC 1 cut(s) 89
AoxI GGCC 3 cut(s) 276, 780, 786
ApeKI GCWGC 3 cut(s) 497, 594, 722
ApoI RAATTY 4 cut(s) 287, 830, 949, 990
ArsI GACNNNNNNTTYG 2 cut(s) 991, 1023
Asp700I GAANNNNTTC 2 cut(s) 288, 747
Asp718I GGTACC 1 cut(s) 378
AspS9I GGNCC 2 cut(s) 873, 1007
AsuHPI GGTGA 5 cut(s) 167, 175, 319, 983, 1074
AsuII TTCGAA 1 cut(s) 997
AvaII GGWCC 2 cut(s) 873, 1007
BalI TGGCCA 2 cut(s) 278, 788
BanI GGYRCC 3 cut(s) 378, 926, 1069
BbvI GCAGC 3 cut(s) 509, 581, 709
BccI CCATC 2 cut(s) 124, 815
BceAI ACGGC 2 cut(s) 75, 767
BciT130I CCWGG 2 cut(s) 967, 1014
BcoDI GTCTC 3 cut(s) 185, 683, 805
BcuI ACTAGT 1 cut(s) 653
BfaI CTAG 2 cut(s) 411, 654
BfmI CTRYAG 1 cut(s) 1088
BisI GCNGC 3 cut(s) 498, 595, 723
BlsI GCNGC 3 cut(s) 499, 596, 724
Bme1390I CCNGG 2 cut(s) 967, 1014
Bme18I GGWCC 2 cut(s) 873, 1007
BmgBI CACGTC 1 cut(s) 78
BmgT120I GGNCC 2 cut(s) 873, 1007
BmiI GGNNCC 5 cut(s) 380, 750, 874, 928, 1071
BmrFI CCNGG 2 cut(s) 967, 1014
BmsI GCATC 3 cut(s) 607, 805, 1067
BpmI CTGGAG 3 cut(s) 54, 442, 996
Bpu14I TTCGAA 1 cut(s) 997
BpuEI CTTGAG 1 cut(s) 322
BsaHI GRCGYC 1 cut(s) 178
BsaJI CCNNGG 4 cut(s) 229, 349, 783, 966
Bsc4I CCNNNNNNNGG 4 cut(s) 451, 676, 936, 1016
Bse1I ACTGG 3 cut(s) 310, 425, 797
Bse3DI GCAATG 2 cut(s) 131, 1029
BseBI CCWGG 2 cut(s) 967, 1014
BseDI CCNNGG 4 cut(s) 229, 349, 783, 966
BseGI GGATG 1 cut(s) 135
BseLI CCNNNNNNNGG 4 cut(s) 451, 676, 936, 1016
BseMI GCAATG 2 cut(s) 131, 1029
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 3 cut(s) 310, 425, 797
BseXI GCAGC 3 cut(s) 509, 581, 709
BsgI GTGCAG 1 cut(s) 702
BshFI GGCC 3 cut(s) 278, 782, 788
BshNI GGYRCC 3 cut(s) 378, 926, 1069
BslFI GGGAC 1 cut(s) 886
BslI CCNNNNNNNGG 4 cut(s) 451, 676, 936, 1016
BsmAI GTCTC 3 cut(s) 185, 683, 805
BsmBI CGTCTC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 886
BsnI GGCC 3 cut(s) 278, 782, 788
Bsp119I TTCGAA 1 cut(s) 997
Bsp143I GATC 3 cut(s) 94, 577, 769
BspACI CCGC 3 cut(s) 597, 870, 1074
BspANI GGCC 3 cut(s) 278, 782, 788
BspCNI CTCAG 1 cut(s) 815
BspLI GGNNCC 5 cut(s) 380, 750, 874, 928, 1071
BspPI GGATC 1 cut(s) 89
BspT104I TTCGAA 1 cut(s) 997
BspT107I GGYRCC 3 cut(s) 378, 926, 1069
BsrBI CCGCTC 1 cut(s) 1076
BsrDI GCAATG 2 cut(s) 131, 1029
BsrI ACTGG 3 cut(s) 310, 425, 797
BssECI CCNNGG 4 cut(s) 229, 349, 783, 966
BssMI GATC 3 cut(s) 94, 577, 769
BssNI GRCGYC 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 229
Bst2UI CCWGG 2 cut(s) 967, 1014
Bst4CI ACNGT 6 cut(s) 138, 155, 546, 569, 737, 1089
Bst6I CTCTTC 1 cut(s) 681
BstACI GRCGYC 1 cut(s) 178
BstBI TTCGAA 1 cut(s) 997
BstDEI CTNAG 2 cut(s) 705, 802
BstDSI CCRYGG 2 cut(s) 349, 783
BstENI CCTNNNNNAGG 1 cut(s) 1014
BstF5I GGATG 1 cut(s) 135
BstKTI GATC 3 cut(s) 97, 580, 772
BstMAI GTCTC 3 cut(s) 185, 683, 805
BstMBI GATC 3 cut(s) 94, 577, 769
BstMWI GCNNNNNNNGC 4 cut(s) 488, 497, 815, 824
BstNI CCWGG 2 cut(s) 967, 1014
BstNSI RCATGY 1 cut(s) 696
BstSCI CCNGG 2 cut(s) 965, 1012
BstSFI CTRYAG 1 cut(s) 1088
BstV1I GCAGC 3 cut(s) 509, 581, 709
BsuRI GGCC 3 cut(s) 278, 782, 788
BtgI CCRYGG 2 cut(s) 349, 783
BtrI CACGTC 1 cut(s) 78
BtsCI GGATG 1 cut(s) 135
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 4 cut(s) 303, 498, 542, 1085
Cfr13I GGNCC 2 cut(s) 873, 1007
CseI GACGC 3 cut(s) 153, 167, 244
Csp6I GTAC 3 cut(s) 379, 907, 1039
CviAII CATG 4 cut(s) 693, 887, 954, 985
CviQI GTAC 3 cut(s) 379, 907, 1039
DdeI CTNAG 2 cut(s) 705, 802
DpnI GATC 3 cut(s) 96, 579, 771
DpnII GATC 3 cut(s) 94, 577, 769
DraI TTTAAA 1 cut(s) 625
EaeI YGGCCR 2 cut(s) 276, 786
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
Eco130I CCWWGG 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 873, 1007
EcoNI CCTNNNNNAGG 1 cut(s) 1014
EcoRI GAATTC 2 cut(s) 949, 990
EcoRII CCWGG 2 cut(s) 965, 1012
EcoT14I CCWWGG 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 229
Esp3I CGTCTC 1 cut(s) 185
FaeI CATG 4 cut(s) 696, 890, 957, 988
FaqI GGGAC 1 cut(s) 886
FatI CATG 4 cut(s) 692, 886, 953, 984
FauI CCCGC 2 cut(s) 863, 1081
Fnu4HI GCNGC 3 cut(s) 498, 595, 723
FokI GGATG 1 cut(s) 142
Fsp4HI GCNGC 3 cut(s) 498, 595, 723
FspBI CTAG 2 cut(s) 411, 654
GluI GCNGC 3 cut(s) 498, 595, 723
GsuI CTGGAG 3 cut(s) 54, 442, 996
HaeIII GGCC 3 cut(s) 278, 782, 788
HgaI GACGC 3 cut(s) 153, 167, 244
Hin1I GRCGYC 1 cut(s) 178
Hin1II CATG 4 cut(s) 696, 890, 957, 988
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 1 cut(s) 648
HphI GGTGA 5 cut(s) 167, 175, 319, 983, 1074
Hpy166II GTNNAC 3 cut(s) 259, 353, 565
Hpy188I TCNGA 6 cut(s) 143, 271, 296, 394, 408, 550
Hpy188III TCNNGA 1 cut(s) 193
Hpy8I GTNNAC 3 cut(s) 259, 353, 565
HpyAV CCTTC 2 cut(s) 917, 1053
HpyCH4III ACNGT 6 cut(s) 138, 155, 546, 569, 737, 1089
HpyCH4IV ACGT 2 cut(s) 77, 358
HpyCH4V TGCA 4 cut(s) 164, 468, 683, 1058
HpyF10VI GCNNNNNNNGC 4 cut(s) 488, 497, 815, 824
HpyF3I CTNAG 2 cut(s) 705, 802
HpySE526I ACGT 2 cut(s) 77, 358
Hsp92I GRCGYC 1 cut(s) 178
Hsp92II CATG 4 cut(s) 696, 890, 957, 988
KpnI GGTACC 1 cut(s) 382
Kzo9I GATC 3 cut(s) 94, 577, 769
LmnI GCTCC 3 cut(s) 322, 824, 1081
Lsp1109I GCAGC 3 cut(s) 509, 581, 709
LweI GCATC 3 cut(s) 607, 805, 1067
MaeI CTAG 2 cut(s) 411, 654
MaeII ACGT 2 cut(s) 77, 358
MaeIII GTNAC 7 cut(s) 155, 325, 439, 527, 569, 695, 892
MalI GATC 3 cut(s) 96, 579, 771
MbiI CCGCTC 1 cut(s) 1076
MboI GATC 3 cut(s) 94, 577, 769
MboII GAAGA 7 cut(s) 208, 296, 626, 671, 698, 958, 986
MfeI CAATTG 2 cut(s) 501, 533
MlsI TGGCCA 2 cut(s) 278, 788
MluNI TGGCCA 2 cut(s) 278, 788
MmeI TCCRAC 3 cut(s) 105, 431, 1076
MnlI CCTC 9 cut(s) 78, 109, 417, 499, 638, 762, 1010, 1020, 1090
Mox20I TGGCCA 2 cut(s) 278, 788
MroXI GAANNNNTTC 2 cut(s) 288, 747
MscI TGGCCA 2 cut(s) 278, 788
MseI TTAA 4 cut(s) 251, 332, 585, 624
MslI CAYNNNNRTG 2 cut(s) 191, 1034
Msp20I TGGCCA 2 cut(s) 278, 788
MspR9I CCNGG 2 cut(s) 967, 1014
MunI CAATTG 2 cut(s) 501, 533
MvaI CCWGG 2 cut(s) 967, 1014
MwoI GCNNNNNNNGC 4 cut(s) 488, 497, 815, 824
NdeII GATC 3 cut(s) 94, 577, 769
NlaIII CATG 4 cut(s) 696, 890, 957, 988
NlaIV GGNNCC 5 cut(s) 380, 750, 874, 928, 1071
NmuCI GTSAC 3 cut(s) 155, 325, 695
NspI RCATGY 1 cut(s) 696
NspV TTCGAA 1 cut(s) 997
PciI ACATGT 1 cut(s) 692
PdmI GAANNNNTTC 2 cut(s) 288, 747
PfeI GAWTC 1 cut(s) 648
PflMI CCANNNNNTGG 1 cut(s) 936
PfoI TCCNGGA 1 cut(s) 1012
PkrI GCNGC 3 cut(s) 499, 596, 724
PscI ACATGT 1 cut(s) 692
PsiI TTATAA 1 cut(s) 26
Psp6I CCWGG 2 cut(s) 965, 1012
PspGI CCWGG 2 cut(s) 965, 1012
PspN4I GGNNCC 5 cut(s) 380, 750, 874, 928, 1071
PspPI GGNCC 2 cut(s) 873, 1007
RsaI GTAC 3 cut(s) 380, 908, 1040
RsaNI GTAC 3 cut(s) 379, 907, 1039
RseI CAYNNNNRTG 2 cut(s) 191, 1034
SaqAI TTAA 4 cut(s) 251, 332, 585, 624
SatI GCNGC 3 cut(s) 498, 595, 723
Sau3AI GATC 3 cut(s) 94, 577, 769
Sau96I GGNCC 2 cut(s) 873, 1007
ScrFI CCNGG 2 cut(s) 967, 1014
SfaNI GCATC 3 cut(s) 607, 805, 1067
SfcI CTRYAG 1 cut(s) 1088
SfuI TTCGAA 1 cut(s) 997
SinI GGWCC 2 cut(s) 873, 1007
SmiMI CAYNNNNRTG 2 cut(s) 191, 1034
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
SpeI ACTAGT 1 cut(s) 653
SsiI CCGC 3 cut(s) 597, 870, 1074
SspMI CTAG 2 cut(s) 411, 654
StyD4I CCNGG 2 cut(s) 965, 1012
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 6 cut(s) 138, 155, 546, 569, 737, 1089
TaiI ACGT 2 cut(s) 80, 361
TaqI TCGA 2 cut(s) 101, 997
TfiI GAWTC 1 cut(s) 648
Tru1I TTAA 4 cut(s) 251, 332, 585, 624
Tru9I TTAA 4 cut(s) 251, 332, 585, 624
TscAI CASTG 4 cut(s) 310, 498, 549, 1092
TseFI GTSAC 3 cut(s) 155, 325, 695
TseI GCWGC 3 cut(s) 497, 594, 722
Tsp45I GTSAC 3 cut(s) 155, 325, 695
TspDTI ATGAA 4 cut(s) 102, 297, 756, 942
TspGWI ACGGA 3 cut(s) 338, 657, 924
TspRI CASTG 4 cut(s) 310, 498, 549, 1092
Van91I CCANNNNNTGG 1 cut(s) 936
VpaK11BI GGWCC 2 cut(s) 873, 1007
XagI CCTNNNNNAGG 1 cut(s) 1014
XapI RAATTY 4 cut(s) 287, 830, 949, 990
XceI RCATGY 1 cut(s) 696
XmnI GAANNNNTTC 2 cut(s) 288, 747
XspI CTAG 2 cut(s) 411, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.