FvH4_6g10421

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002962
Physical Location & Seq
Reverse (-)
6254893 .. 6257878
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10421.t1

Sequence Viewer

Length: 1305 bp
ATGGTCAGATACTCAGATACCAAATCATTTTCTCGGCTAGCCTTCTTGTGCAACTTGGCTTATATCATACCAGAGATGAAGGCCACGGATTTGAAGAGATACTATGGCCTACAATTTGTAACATCTTCAATAGATAAGCAAGCGCAGGCAGATCACATTGTGACAATCAAACCAAAACTTGATCAGGATTCTACTGCAGTGAAATCTATGGCCTCAGCTGAAGCTAATCTCCGCTCGCTAAGAAAATCACAGCAGGATGCAGGAGAGAATTCATGCAGAAATGGAGGGAGCTCACCCCAAAATCACAAGTCTGATAAATATGAAAACATATCAACATCAAAGCAAGGAGCAGAAAAGGACTTTCAGTCACTGCACTTACCTTGTGAGTGGTTTGTTTGTGATGATCCCACTACTTATACTCGTTTTTGTGTGATTCAGGGTATAGACTCCCTTGCATCGTGGCAGGCATATTTCTTATTTGAACCCATGGAATTTGAGGGGACTACTGTACTTGCTCACCGAGGAATTTATGAACTGGCAAAGAAACTTTATGAGCATTTCATTCCTGAAATAATCGACCATTTGATAAGACATGGTGAGCAAGCGAAGCTTCAATTTACTGGCCATTGTCTTGGAGGAAGCCTCTCTGTTATACTCCAGTTGATGCTACTGACTAGGAAGGTCGTCAGTCCCTCAAATCTTCTGCCTGTCGTCACATTTGGCTCCCCATATGTGTTCTGTGGAGGCCAGAAAATATTTGAGCAACTAAGATTGGATGAGTGCAATCACATCCGTTCTGTTATGCTGCACAGAGATATTGTCCCTAGATCTTTCTCCTCCAAAATTTCTGATCATGTCACAGCAATTCTCAAGAGTTTACATGGTCCATTAGAGTCACACCCTTGTCTAACAAAGAATAAGATATTGTACTCTCCCATGGGTAAACTGTTTATTGTCCAACCTGATGAAATGTCATCTCCTCCACACCCTCTACTCCCGTTAGGTAGCGCTCTCTACGCTTTGAACAGTACCCGAGATGGATTCTCCTTGAGTGTATTGATGGACTTTTTGAATTCCCCACATCCACTAGAAACACCAAGTGATCCTACAGCATATGGTTCAGAAGGTACAATTATAAGGGATCATGACTCGAGCAACTATCTGAAGGCCATGAATGGGATTGTAAGGCTAAACACCAGAATTCTCCGGACAGCAAGGAAGAGGAGTAATATCTTGTGGCCGCTGCTTAATTCACCACCACCAAGCTCATGGAACCATGAAGATGAAAGCGACATAGAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

435

Amino Acids

48.76

Weight (kDa)

6.99

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 144 - 278 3.1e-20 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1136
AccBSI CCGCTC 1 cut(s) 234
AccIII TCCGGA 1 cut(s) 1206
AciI CCGC 2 cut(s) 232, 1241
AclWI GGATC 3 cut(s) 398, 1097, 1149
AcoI YGGCCR 2 cut(s) 622, 1238
AcsI RAATTY 6 cut(s) 268, 491, 525, 843, 1072, 1200
AcuI CTGAAG 2 cut(s) 240, 1184
AdeI CACNNNGTG 2 cut(s) 160, 1100
AfaI GTAC 4 cut(s) 510, 929, 1030, 1129
AfeI AGCGCT 1 cut(s) 1009
AfiI CCNNNNNNNGG 1 cut(s) 1176
AgsI TTSAA 6 cut(s) 94, 129, 482, 614, 1024, 1072
AhdI GACNNNNNGTC 1 cut(s) 364
AluBI AGCT 6 cut(s) 218, 224, 291, 610, 1266, 1302
AluI AGCT 6 cut(s) 218, 224, 291, 610, 1266, 1302
Alw21I GWGCWC 1 cut(s) 293
AlwI GGATC 3 cut(s) 398, 1097, 1149
AlwNI CAGNNNCTG 1 cut(s) 370
Ama87I CYCGRG 2 cut(s) 1032, 1150
Aor13HI TCCGGA 1 cut(s) 1206
Aor51HI AGCGCT 1 cut(s) 1009
AoxI GGCC 7 cut(s) 81, 106, 210, 622, 745, 1167, 1238
ApeKI GCWGC 2 cut(s) 805, 1243
ApoI RAATTY 6 cut(s) 268, 491, 525, 843, 1072, 1200
AspLEI GCGC 2 cut(s) 145, 1010
AspS9I GGNCC 1 cut(s) 884
AsuHPI GGTGA 4 cut(s) 285, 509, 608, 1245
AsuNHI GCTAGC 1 cut(s) 37
AvaI CYCGRG 2 cut(s) 1032, 1150
AvaII GGWCC 1 cut(s) 884
BalI TGGCCA 1 cut(s) 624
BanII GRGCYC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 293
BbvCI CCTCAGC 1 cut(s) 214
BbvI GCAGC 2 cut(s) 792, 1230
BccI CCATC 2 cut(s) 1031, 1054
BclI TGATCA 2 cut(s) 181, 850
BfaI CTAG 5 cut(s) 38, 675, 825, 1088, 1303
BfmI CTRYAG 2 cut(s) 195, 1107
BfoI RGCGCY 1 cut(s) 1011
BglII AGATCT 1 cut(s) 827
BisI GCNGC 3 cut(s) 806, 1241, 1244
BlsI GCNGC 3 cut(s) 807, 1242, 1245
Bme18I GGWCC 1 cut(s) 884
BmeRI GACNNNNNGTC 1 cut(s) 364
BmeT110I CYCGRG 2 cut(s) 1032, 1150
BmgT120I GGNCC 1 cut(s) 884
BmiI GGNNCC 2 cut(s) 724, 1274
BmsI GCATC 3 cut(s) 247, 464, 654
BmtI GCTAGC 1 cut(s) 41
BplI GAGNNNNNCTC 2 cut(s) 627, 659
BpmI CTGGAG 1 cut(s) 641
Bpu10I CCTNAGC 1 cut(s) 214
BpuEI CTTGAG 2 cut(s) 854, 1069
BsaJI CCNNGG 4 cut(s) 84, 486, 520, 936
BsaWI WCCGGW 1 cut(s) 1206
Bsc4I CCNNNNNNNGG 1 cut(s) 1176
Bse1I ACTGG 3 cut(s) 540, 625, 658
BseAI TCCGGA 1 cut(s) 1206
BseDI CCNNGG 4 cut(s) 84, 486, 520, 936
BseGI GGATG 4 cut(s) 262, 781, 789, 1081
BseLI CCNNNNNNNGG 1 cut(s) 1176
BseMII CTCAG 2 cut(s) 27, 228
BseNI ACTGG 3 cut(s) 540, 625, 658
BseRI GAGGAG 3 cut(s) 826, 969, 1237
BseXI GCAGC 2 cut(s) 792, 1230
BsgI GTGCAG 2 cut(s) 356, 791
BshFI GGCC 7 cut(s) 83, 108, 212, 624, 747, 1169, 1240
BsiHKAI GWGCWC 1 cut(s) 293
BsiHKCI CYCGRG 2 cut(s) 1032, 1150
BsiSI CCGG 1 cut(s) 1207
BslFI GGGAC 3 cut(s) 514, 675, 806
BslI CCNNNNNNNGG 1 cut(s) 1176
BsmFI GGGAC 3 cut(s) 514, 675, 806
BsnI GGCC 7 cut(s) 83, 108, 212, 624, 747, 1169, 1240
BsoBI CYCGRG 2 cut(s) 1032, 1150
Bsp1286I GDGCHC 1 cut(s) 293
Bsp13I TCCGGA 1 cut(s) 1206
Bsp143I GATC 7 cut(s) 151, 181, 403, 827, 850, 1102, 1141
Bsp19I CCATGG 2 cut(s) 486, 936
BspACI CCGC 2 cut(s) 232, 1241
BspANI GGCC 7 cut(s) 83, 108, 212, 624, 747, 1169, 1240
BspCNI CTCAG 2 cut(s) 26, 227
BspEI TCCGGA 1 cut(s) 1206
BspHI TCATGA 1 cut(s) 1144
BspLI GGNNCC 2 cut(s) 724, 1274
BspMAI CTGCAG 1 cut(s) 199
BspOI GCTAGC 1 cut(s) 41
BspPI GGATC 3 cut(s) 398, 1097, 1149
BsrBI CCGCTC 1 cut(s) 234
BsrI ACTGG 3 cut(s) 540, 625, 658
BssECI CCNNGG 4 cut(s) 84, 486, 520, 936
BssMI GATC 7 cut(s) 151, 181, 403, 827, 850, 1102, 1141
BssT1I CCWWGG 2 cut(s) 486, 936
Bst4CI ACNGT 3 cut(s) 508, 948, 1028
Bst6I CTCTTC 2 cut(s) 89, 1214
BstC8I GCNNGC 6 cut(s) 39, 141, 147, 236, 465, 603
BstDEI CTNAG 4 cut(s) 13, 214, 239, 767
BstDSI CCRYGG 3 cut(s) 84, 486, 936
BstF5I GGATG 4 cut(s) 262, 781, 789, 1081
BstH2I RGCGCY 1 cut(s) 1011
BstHHI GCGC 2 cut(s) 145, 1010
BstKTI GATC 7 cut(s) 154, 184, 406, 830, 853, 1105, 1144
BstMBI GATC 7 cut(s) 151, 181, 403, 827, 850, 1102, 1141
BstMWI GCNNNNNNNGC 2 cut(s) 607, 1016
BstSFI CTRYAG 2 cut(s) 195, 1107
BstV1I GCAGC 2 cut(s) 792, 1230
BstX2I RGATCY 1 cut(s) 827
BstXI CCANNNNNNTGG 2 cut(s) 632, 1269
BstYI RGATCY 1 cut(s) 827
BsuRI GGCC 7 cut(s) 83, 108, 212, 624, 747, 1169, 1240
BtgI CCRYGG 3 cut(s) 84, 486, 936
BtsCI GGATG 4 cut(s) 262, 781, 789, 1081
BtsI GCAGTG 2 cut(s) 204, 368
BtsIMutI CAGTG 2 cut(s) 204, 368
Cac8I GCNNGC 6 cut(s) 39, 141, 147, 236, 465, 603
CaiI CAGNNNCTG 1 cut(s) 370
CciI TCATGA 1 cut(s) 1144
CfoI GCGC 2 cut(s) 145, 1010
Cfr13I GGNCC 1 cut(s) 884
Csp6I GTAC 4 cut(s) 509, 928, 1029, 1128
CviQI GTAC 4 cut(s) 509, 928, 1029, 1128
DdeI CTNAG 4 cut(s) 13, 214, 239, 767
DpnI GATC 7 cut(s) 153, 183, 405, 829, 852, 1104, 1143
DpnII GATC 7 cut(s) 151, 181, 403, 827, 850, 1102, 1141
DraIII CACNNNGTG 2 cut(s) 160, 1100
DriI GACNNNNNGTC 1 cut(s) 364
EaeI YGGCCR 2 cut(s) 622, 1238
Eam1104I CTCTTC 2 cut(s) 89, 1214
Eam1105I GACNNNNNGTC 1 cut(s) 364
EarI CTCTTC 2 cut(s) 89, 1214
Ecl136II GAGCTC 1 cut(s) 291
Eco130I CCWWGG 2 cut(s) 486, 936
Eco24I GRGCYC 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 884
Eco47III AGCGCT 1 cut(s) 1009
Eco53kI GAGCTC 1 cut(s) 291
Eco57I CTGAAG 2 cut(s) 240, 1184
Eco88I CYCGRG 2 cut(s) 1032, 1150
EcoICRI GAGCTC 1 cut(s) 291
EcoRI GAATTC 3 cut(s) 268, 1072, 1200
EcoT14I CCWWGG 2 cut(s) 486, 936
EcoT38I GRGCYC 1 cut(s) 293
ErhI CCWWGG 2 cut(s) 486, 936
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 3 cut(s) 514, 675, 806
FauNDI CATATG 2 cut(s) 730, 1114
FbaI TGATCA 2 cut(s) 181, 850
Fnu4HI GCNGC 3 cut(s) 806, 1241, 1244
FokI GGATG 4 cut(s) 269, 776, 788, 1068
FriOI GRGCYC 1 cut(s) 293
Fsp4HI GCNGC 3 cut(s) 806, 1241, 1244
FspBI CTAG 5 cut(s) 38, 675, 825, 1088, 1303
GlaI GCGC 2 cut(s) 144, 1009
GluI GCNGC 3 cut(s) 806, 1241, 1244
GsuI CTGGAG 1 cut(s) 641
HaeII RGCGCY 1 cut(s) 1011
HaeIII GGCC 7 cut(s) 83, 108, 212, 624, 747, 1169, 1240
HapII CCGG 1 cut(s) 1207
HhaI GCGC 2 cut(s) 145, 1010
Hin6I GCGC 2 cut(s) 143, 1008
HinP1I GCGC 2 cut(s) 143, 1008
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 6 cut(s) 188, 433, 446, 893, 1041, 1148
HpaII CCGG 1 cut(s) 1207
HphI GGTGA 4 cut(s) 285, 509, 608, 1245
Hpy166II GTNNAC 2 cut(s) 878, 944
Hpy188I TCNGA 6 cut(s) 8, 16, 313, 850, 1123, 1164
Hpy188III TCNNGA 5 cut(s) 185, 566, 871, 1145, 1207
Hpy8I GTNNAC 2 cut(s) 878, 944
HpyAV CCTTC 5 cut(s) 52, 73, 673, 1118, 1159
HpyCH4III ACNGT 3 cut(s) 508, 948, 1028
HpyCH4V TGCA 8 cut(s) 51, 197, 260, 276, 373, 455, 783, 808
HpyF10VI GCNNNNNNNGC 2 cut(s) 607, 1016
HpyF3I CTNAG 4 cut(s) 13, 214, 239, 767
HspAI GCGC 2 cut(s) 143, 1008
Kpn2I TCCGGA 1 cut(s) 1206
Ksp22I TGATCA 2 cut(s) 181, 850
Kzo9I GATC 7 cut(s) 151, 181, 403, 827, 850, 1102, 1141
LmnI GCTCC 3 cut(s) 288, 347, 728
Lsp1109I GCAGC 2 cut(s) 792, 1230
LweI GCATC 3 cut(s) 247, 464, 654
MaeI CTAG 5 cut(s) 38, 675, 825, 1088, 1303
MaeIII GTNAC 6 cut(s) 118, 160, 366, 712, 856, 894
MalI GATC 7 cut(s) 153, 183, 405, 829, 852, 1104, 1143
MbiI CCGCTC 1 cut(s) 234
MboI GATC 7 cut(s) 151, 181, 403, 827, 850, 1102, 1141
MboII GAAGA 5 cut(s) 106, 117, 692, 1231, 1292
MflI RGATCY 1 cut(s) 827
MhlI GDGCHC 1 cut(s) 293
MlsI TGGCCA 1 cut(s) 624
MluNI TGGCCA 1 cut(s) 624
MlyI GAGTC 3 cut(s) 440, 902, 1142
MmeI TCCRAC 1 cut(s) 982
Mox20I TGGCCA 1 cut(s) 624
MroI TCCGGA 1 cut(s) 1206
MscI TGGCCA 1 cut(s) 624
MseI TTAA 1 cut(s) 1248
MslI CAYNNNNRTG 1 cut(s) 1281
Msp20I TGGCCA 1 cut(s) 624
MspA1I CMGCKG 2 cut(s) 218, 1243
MspI CCGG 1 cut(s) 1207
MwoI GCNNNNNNNGC 2 cut(s) 607, 1016
NcoI CCATGG 2 cut(s) 486, 936
NdeI CATATG 2 cut(s) 730, 1114
NdeII GATC 7 cut(s) 151, 181, 403, 827, 850, 1102, 1141
NheI GCTAGC 1 cut(s) 37
NlaIV GGNNCC 2 cut(s) 724, 1274
NmeAIII GCCGAG 1 cut(s) 13
NmuCI GTSAC 5 cut(s) 160, 366, 712, 856, 894
PaeR7I CTCGAG 1 cut(s) 1150
PagI TCATGA 1 cut(s) 1144
PfeI GAWTC 3 cut(s) 188, 433, 1041
PkrI GCNGC 3 cut(s) 807, 1242, 1245
PleI GAGTC 3 cut(s) 440, 901, 1142
PpsI GAGTC 3 cut(s) 440, 901, 1142
PsiI TTATAA 1 cut(s) 1136
Psp124BI GAGCTC 1 cut(s) 293
PspN4I GGNNCC 2 cut(s) 724, 1274
PspPI GGNCC 1 cut(s) 884
PspXI VCTCGAGB 1 cut(s) 1150
PstI CTGCAG 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 827
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 4 cut(s) 510, 929, 1030, 1129
RsaNI GTAC 4 cut(s) 509, 928, 1029, 1128
RseI CAYNNNNRTG 1 cut(s) 1281
SacI GAGCTC 1 cut(s) 293
SaqAI TTAA 1 cut(s) 1248
SatI GCNGC 3 cut(s) 806, 1241, 1244
Sau3AI GATC 7 cut(s) 151, 181, 403, 827, 850, 1102, 1141
Sau96I GGNCC 1 cut(s) 884
SchI GAGTC 3 cut(s) 440, 902, 1142
SduI GDGCHC 1 cut(s) 293
SfaNI GCATC 3 cut(s) 247, 464, 654
SfcI CTRYAG 2 cut(s) 195, 1107
Sfr274I CTCGAG 1 cut(s) 1150
SinI GGWCC 1 cut(s) 884
SlaI CTCGAG 1 cut(s) 1150
SmiMI CAYNNNNRTG 1 cut(s) 1281
SmlI CTYRAG 3 cut(s) 869, 1048, 1150
SmoI CTYRAG 3 cut(s) 869, 1048, 1150
SsiI CCGC 2 cut(s) 232, 1241
SspI AATATT 1 cut(s) 756
SspMI CTAG 5 cut(s) 38, 675, 825, 1088, 1303
SstI GAGCTC 1 cut(s) 293
StyI CCWWGG 2 cut(s) 486, 936
TaaI ACNGT 3 cut(s) 508, 948, 1028
TaqI TCGA 2 cut(s) 576, 1151
TatI WGTACW 2 cut(s) 508, 927
TauI GCSGC 1 cut(s) 1243
TfiI GAWTC 3 cut(s) 188, 433, 1041
Tru1I TTAA 1 cut(s) 1248
Tru9I TTAA 1 cut(s) 1248
TscAI CASTG 2 cut(s) 204, 375
TseFI GTSAC 5 cut(s) 160, 366, 712, 856, 894
TseI GCWGC 2 cut(s) 805, 1243
Tsp45I GTSAC 5 cut(s) 160, 366, 712, 856, 894
TspDTI ATGAA 9 cut(s) 92, 261, 336, 546, 550, 981, 1187, 1293, 1299
TspGWI ACGGA 2 cut(s) 101, 782
TspRI CASTG 2 cut(s) 204, 375
VpaK11BI GGWCC 1 cut(s) 884
XapI RAATTY 6 cut(s) 268, 491, 525, 843, 1072, 1200
XcmI CCANNNNNNNNNTGG 1 cut(s) 1266
XhoI CTCGAG 1 cut(s) 1150
XspI CTAG 5 cut(s) 38, 675, 825, 1088, 1303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.