RchiOBHm_Chr1g0359261

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002962
Physical Location & Seq
Forward (+)
51282805 .. 51285300
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58432

Sequence Viewer

Length: 2001 bp
ATGGCCTGCACTTCATTATCTATACCTAGCTCTCCGGCTTCAACGAGGACTATAGACGTCTTCAAGGAGCAGAGTGGTCTTCGGCGATCGCAATCCAGCAAAGACCTTTACAGACGTGCAAATATTCGGAGGTCTTATTCCGATAACCATCTTTGCTACTGCATTAGGCCTATTCATGCTGCGTCAGCCCAACCAAAACTAAAGAACAGCCGGTCCATGGGGTTTTTCCCCTTTCAATTTTCGAGCTCCATTCTACCAAACTCGGTTAGATCATTTTTGTTTGATGCAGACACGAGTAAGCATACGAGCACGGCAGAGAGGGAAATGGAAATGATCGATAACTCTAGGGAGAGCGGTGAAGAAGAAGAAGAAGAAGATCATGAGATAAAGAGAGCCAATTGGGTGGAGAGGCTTGTGGAGATCAGAAGAAAATGGAGTAATAGGCAGCAAAAAGAAGTTGGGGATGAAGACAGTGTTTGTCACGAAGGTGATGGTGATTGTGAATGTGGAGAGGGTGGTTGTTTAGTGGATTACAGTACAGATGAAGATGATGGAGAGATAACTTCAGAAAGTTTCCAAAGGTTATTGGTCAAGGTACCATGGTCTGATACCAAACAATTTTCTCAGCTAGCCTTCTTGAGCAACCTGGCATATGTAATACCATTGATAAAGGCCGATGATTTGAAGAGATATTACGGCCTACAGTTTGTAACATCTTCATTAGTAAAGAAAGCAGAGGAAGAGGAACTCAAAGAAGGAATTGATCAGGACTCTACTCGTGTACCTGTAACTGACGAGGAATCCACTTCAGAGGCAGCTGCAGACTCTGAGCAGAAGCATTCAATCCATCCATCTGTTGCTTACGAAATTGCTGCCTCAGCAGCATCTTATGTTCGCTCGCGTGACATGGATGGTGGTGATGTAGAGAATACATTCAGAAGTGGAGACCAACAACAACAACGAGAGGAAGGAGGGAGCTCCCCCCGAAATTATAAGTCGGAGGTAGCTGTTTCCTTAACAGCATCAACGATGACAGTCGTGGTTGCAGCAGGGGAAAAACAAAAGCAAGAAACAGCACAAGACCTTCGGTCACCCCAATCTGCACCTTGTGAATGGTTTGTTTGTGATGACAACAGTACATATACTCGCTGCTTTGTGATTCAGGGAACAAATGTACTTGTTCACAGAGGAATCTATGAAGCAGCAAAGGGGATGTACGAGCAATTCATGCCACAAATAATCGAGCACTTGGATAGATATGGAGAGCGTGCAAAGTTTCAGTTCACTGGTCATTCTCTTGGAGGAAGCCTCTCTCTTCTAGTCCACTTGATGTTACTGACCAGGAACTTTGTCAAGCCCTCCACTCTTCGGCCAGTTGTGACATTTGGCTCACCATTTGTGTTTTGTGGAGGCCAGAGAATACTAGACGACCTGGGTGTCGATGAGAACCAAATCCATTGCGTGATGATGCATAGAGATATTGTCCCAAGAGCCTTCTCCTGCAACTACCCTAACTATGTGGCTACGCTTCTCAAGCGTTTAAATGGTTCATTTCGATCACACCCTTGTCTGATAAAAAACAAAATATTGTACTCTCCTATGGGTAAAATATTCATTCTGCAACCTGATGAAAGCTCGTCTCCGCCACACCCTCTTCTCCCCTTGGGGAGTGCCCTCTATGCTTTGGACAAAACTCAACATGGGTACTCTTCAAGCGTCCTTAGAACCTTCTTAAATTCCCCGCATCCGCTAGAAACCCTAAGCGATCCTACAGCTTATGGTTCGGAAGGTACAATCCTACGGGATCATGACTCGAGCAACTATCTGAAGGCTGTGAATGGTGTCCTTAGGCTACACACAAGAAAGATTGTTCGTATAGCTAGGAAACAGAGGAATGTCTTGTGGCCGCTACTTACTTCACCATCCCCACACACATGGAACCATGAAAATAATCTGGAGAGCGGCAGATTGGTGACGAAGGAGATTCTCACAGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

666

Amino Acids

74.85

Weight (kDa)

5.94

Isoelectric Point (pI)

59.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 351 - 502 1.2e-21 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
AasI GACNNNNNNGTC 1 cut(s) 1436
AatII GACGTC 1 cut(s) 60
Acc65I GGTACC 1 cut(s) 595
AccB1I GGYRCC 1 cut(s) 595
AccBSI CCGCTC 2 cut(s) 354, 1962
AccII CGCG 1 cut(s) 901
AciI CCGC 6 cut(s) 354, 1643, 1742, 1748, 1907, 1962
AclWI GGATC 2 cut(s) 1760, 1812
AcoI YGGCCR 2 cut(s) 1370, 1904
AcsI RAATTY 1 cut(s) 1735
AcuI CTGAAG 3 cut(s) 549, 792, 1847
AcyI GRCGYC 1 cut(s) 57
AdeI CACNNNGTG 1 cut(s) 1109
AfaI GTAC 9 cut(s) 538, 597, 783, 1138, 1176, 1217, 1592, 1706, 1792
AfiI CCNNNNNNNGG 2 cut(s) 217, 1368
AgsI TTSAA 6 cut(s) 42, 64, 236, 685, 843, 1713
AhdI GACNNNNNGTC 1 cut(s) 1087
AjiI CACGTC 1 cut(s) 116
AjnI CCWGG 3 cut(s) 645, 1340, 1431
AleI CACNNNNGTG 1 cut(s) 486
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 9 cut(s) 30, 246, 628, 818, 978, 1007, 1635, 1775, 1880
AluI AGCT 9 cut(s) 30, 246, 628, 818, 978, 1007, 1635, 1775, 1880
Alw21I GWGCWC 4 cut(s) 248, 311, 980, 1248
Alw26I GTCTC 2 cut(s) 939, 1644
AlwI GGATC 2 cut(s) 1760, 1812
AlwNI CAGNNNCTG 1 cut(s) 827
Ama87I CYCGRG 1 cut(s) 1813
AoxI GGCC 7 cut(s) 3, 167, 672, 697, 1370, 1411, 1904
ApeKI GCWGC 9 cut(s) 179, 445, 815, 818, 872, 881, 1046, 1149, 1202
ApoI RAATTY 1 cut(s) 1735
AsiSI GCGATCGC 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 932
Asp718I GGTACC 1 cut(s) 595
AspS9I GGNCC 1 cut(s) 213
AsuHPI GGTGA 8 cut(s) 368, 500, 506, 929, 1083, 1383, 1911, 1984
AsuNHI GCTAGC 1 cut(s) 628
AvaI CYCGRG 1 cut(s) 1813
AvaII GGWCC 1 cut(s) 213
AxyI CCTNAGG 1 cut(s) 1847
BaeGI GKGCMC 1 cut(s) 1675
BaeI ACNNNNGTAYC 4 cut(s) 587, 620, 765, 798
BanI GGYRCC 1 cut(s) 595
BanII GRGCYC 2 cut(s) 248, 980
BarI GAAGNNNNNNTAC 2 cut(s) 677, 709
BauI CACGAG 2 cut(s) 292, 777
BbsI GAAGAC 3 cut(s) 52, 71, 474
Bbv12I GWGCWC 4 cut(s) 248, 311, 980, 1248
BbvCI CCTCAGC 1 cut(s) 877
BbvI GCAGC 9 cut(s) 166, 457, 805, 827, 859, 893, 1058, 1136, 1214
BccI CCATC 7 cut(s) 156, 485, 545, 855, 859, 905, 1930
BceAI ACGGC 2 cut(s) 327, 712
BcgI CGANNNNNNTGC 2 cut(s) 854, 888
BciT130I CCWGG 3 cut(s) 647, 1342, 1433
BclI TGATCA 1 cut(s) 763
BcoDI GTCTC 2 cut(s) 939, 1644
BfaI CTAG 7 cut(s) 27, 345, 629, 1319, 1424, 1751, 1881
BfmI CTRYAG 4 cut(s) 51, 701, 819, 1770
Bme1390I CCNGG 3 cut(s) 647, 1342, 1433
Bme18I GGWCC 1 cut(s) 213
BmeRI GACNNNNNGTC 1 cut(s) 1087
BmeT110I CYCGRG 1 cut(s) 1813
BmgBI CACGTC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 2 cut(s) 597, 1940
BmrFI CCNGG 3 cut(s) 647, 1342, 1433
BmsI GCATC 5 cut(s) 274, 893, 1031, 1458, 1753
BmtI GCTAGC 1 cut(s) 632
BpiI GAAGAC 3 cut(s) 52, 71, 474
BplI GAGNNNNNCTC 2 cut(s) 1293, 1325
BpmI CTGGAG 1 cut(s) 1976
Bpu10I CCTNAGC 2 cut(s) 877, 1760
BpuEI CTTGAG 2 cut(s) 658, 1517
Bsa29I ATCGAT 1 cut(s) 336
BsaBI GATNNNNATC 2 cut(s) 91, 147
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 939
BsaJI CCNNGG 4 cut(s) 216, 599, 1432, 1662
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 1368
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 2 cut(s) 1291, 1373
Bse21I CCTNAGG 1 cut(s) 1847
Bse3DI GCAATG 1 cut(s) 1456
Bse8I GATNNNNATC 2 cut(s) 91, 147
BseBI CCWGG 3 cut(s) 647, 1342, 1433
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 4 cut(s) 216, 599, 1432, 1662
BseGI GGATG 6 cut(s) 469, 847, 916, 1218, 1744, 1922
BseJI GATNNNNATC 2 cut(s) 91, 147
BseLI CCNNNNNNNGG 2 cut(s) 217, 1368
BseMI GCAATG 1 cut(s) 1456
BseMII CTCAG 3 cut(s) 638, 819, 891
BseNI ACTGG 2 cut(s) 1291, 1373
BseSI GKGCMC 1 cut(s) 1675
BseXI GCAGC 9 cut(s) 166, 457, 805, 827, 859, 893, 1058, 1136, 1214
BsgI GTGCAG 1 cut(s) 1086
Bsh1236I CGCG 1 cut(s) 901
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 7 cut(s) 5, 169, 674, 699, 1372, 1413, 1906
BshNI GGYRCC 1 cut(s) 595
BshVI ATCGAT 1 cut(s) 336
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 4 cut(s) 248, 311, 980, 1248
BsiHKCI CYCGRG 1 cut(s) 1813
BsiSI CCGG 2 cut(s) 35, 211
BslFI GGGAC 1 cut(s) 1469
BslI CCNNNNNNNGG 2 cut(s) 217, 1368
BsmAI GTCTC 2 cut(s) 939, 1644
BsmBI CGTCTC 1 cut(s) 1644
BsmFI GGGAC 1 cut(s) 1469
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 7 cut(s) 5, 169, 674, 699, 1372, 1413, 1906
Bso31I GGTCTC 1 cut(s) 939
BsoBI CYCGRG 1 cut(s) 1813
Bsp1286I GDGCHC 5 cut(s) 248, 311, 980, 1248, 1675
Bsp143I GATC 9 cut(s) 86, 269, 333, 376, 420, 763, 1556, 1765, 1804
Bsp19I CCATGG 2 cut(s) 216, 599
BspACI CCGC 6 cut(s) 354, 1643, 1742, 1748, 1907, 1962
BspANI GGCC 7 cut(s) 5, 169, 674, 699, 1372, 1413, 1906
BspCNI CTCAG 3 cut(s) 637, 820, 890
BspDI ATCGAT 1 cut(s) 336
BspFNI CGCG 1 cut(s) 901
BspHI TCATGA 2 cut(s) 379, 1807
BspLI GGNNCC 2 cut(s) 597, 1940
BspMAI CTGCAG 1 cut(s) 823
BspOI GCTAGC 1 cut(s) 632
BspPI GGATC 2 cut(s) 1760, 1812
BspT107I GGYRCC 1 cut(s) 595
BspTNI GGTCTC 1 cut(s) 939
BsrBI CCGCTC 2 cut(s) 354, 1962
BsrDI GCAATG 1 cut(s) 1456
BsrFI RCCGGY 1 cut(s) 210
BsrI ACTGG 2 cut(s) 1291, 1373
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 4 cut(s) 216, 599, 1432, 1662
BssMI GATC 9 cut(s) 86, 269, 333, 376, 420, 763, 1556, 1765, 1804
BssNI GRCGYC 1 cut(s) 57
BssSI CACGAG 2 cut(s) 292, 777
BssT1I CCWWGG 3 cut(s) 216, 599, 1662
Bst2BI CACGAG 2 cut(s) 292, 777
Bst2UI CCWGG 3 cut(s) 647, 1342, 1433
Bst4CI ACNGT 5 cut(s) 473, 536, 705, 1036, 1136
Bst6I CTCTTC 6 cut(s) 680, 735, 1320, 1371, 1659, 1714
BstACI GRCGYC 1 cut(s) 57
BstAPI GCANNNNNTGC 1 cut(s) 1228
BstC8I GCNNGC 4 cut(s) 7, 630, 899, 1269
BstDEI CTNAG 6 cut(s) 624, 828, 877, 1721, 1760, 1847
BstDSI CCRYGG 2 cut(s) 216, 599
BstEII GGTNACC 1 cut(s) 1089
BstF5I GGATG 6 cut(s) 469, 847, 916, 1218, 1744, 1922
BstFNI CGCG 1 cut(s) 901
BstKTI GATC 9 cut(s) 89, 272, 336, 379, 423, 766, 1559, 1768, 1807
BstMAI GTCTC 2 cut(s) 939, 1644
BstMBI GATC 9 cut(s) 86, 269, 333, 376, 420, 763, 1556, 1765, 1804
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 6 cut(s) 185, 878, 881, 1228, 1534, 1679
BstNI CCWGG 3 cut(s) 647, 1342, 1433
BstPI GGTNACC 1 cut(s) 1089
BstSCI CCNGG 3 cut(s) 645, 1340, 1431
BstSFI CTRYAG 4 cut(s) 51, 701, 819, 1770
BstSLI GKGCMC 1 cut(s) 1675
BstUI CGCG 1 cut(s) 901
BstV1I GCAGC 9 cut(s) 166, 457, 805, 827, 859, 893, 1058, 1136, 1214
BstV2I GAAGAC 3 cut(s) 52, 71, 474
BstXI CCANNNNNNTGG 2 cut(s) 403, 1935
Bsu15I ATCGAT 1 cut(s) 336
Bsu36I CCTNAGG 1 cut(s) 1847
BsuRI GGCC 7 cut(s) 5, 169, 674, 699, 1372, 1413, 1906
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 2 cut(s) 216, 599
BtrI CACGTC 1 cut(s) 116
BtsCI GGATG 6 cut(s) 469, 847, 916, 1218, 1744, 1922
BtsIMutI CAGTG 2 cut(s) 478, 1284
Cac8I GCNNGC 4 cut(s) 7, 630, 899, 1269
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 2 cut(s) 379, 1807
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 213
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 2 cut(s) 171, 1705
Csp6I GTAC 9 cut(s) 537, 596, 782, 1137, 1175, 1216, 1591, 1705, 1791
CviQI GTAC 9 cut(s) 537, 596, 782, 1137, 1175, 1216, 1591, 1705, 1791
DdeI CTNAG 6 cut(s) 624, 828, 877, 1721, 1760, 1847
DpnI GATC 9 cut(s) 88, 271, 335, 378, 422, 765, 1558, 1767, 1806
DpnII GATC 9 cut(s) 86, 269, 333, 376, 420, 763, 1556, 1765, 1804
DraI TTTAAA 1 cut(s) 1542
DraIII CACNNNGTG 1 cut(s) 1109
DrdI GACNNNNNNGTC 1 cut(s) 1436
DriI GACNNNNNGTC 1 cut(s) 1087
DseDI GACNNNNNNGTC 1 cut(s) 1436
EaeI YGGCCR 2 cut(s) 1370, 1904
Eam1104I CTCTTC 6 cut(s) 680, 735, 1320, 1371, 1659, 1714
Eam1105I GACNNNNNGTC 1 cut(s) 1087
EarI CTCTTC 6 cut(s) 680, 735, 1320, 1371, 1659, 1714
EciI GGCGGA 1 cut(s) 1632
Ecl136II GAGCTC 2 cut(s) 246, 978
Eco130I CCWWGG 3 cut(s) 216, 599, 1662
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 2 cut(s) 248, 980
Eco31I GGTCTC 1 cut(s) 939
Eco47I GGWCC 1 cut(s) 213
Eco53kI GAGCTC 2 cut(s) 246, 978
Eco57I CTGAAG 3 cut(s) 549, 792, 1847
Eco81I CCTNAGG 1 cut(s) 1847
Eco88I CYCGRG 1 cut(s) 1813
Eco91I GGTNACC 1 cut(s) 1089
EcoICRI GAGCTC 2 cut(s) 246, 978
EcoO65I GGTNACC 1 cut(s) 1089
EcoRII CCWGG 3 cut(s) 645, 1340, 1431
EcoT14I CCWWGG 3 cut(s) 216, 599, 1662
EcoT22I ATGCAT 1 cut(s) 1473
EcoT38I GRGCYC 2 cut(s) 248, 980
ErhI CCWWGG 3 cut(s) 216, 599, 1662
Esp3I CGTCTC 1 cut(s) 1644
FaqI GGGAC 1 cut(s) 1469
FauI CCCGC 1 cut(s) 1749
FauNDI CATATG 1 cut(s) 652
FbaI TGATCA 1 cut(s) 763
FokI GGATG 6 cut(s) 476, 834, 923, 1225, 1731, 1909
FriOI GRGCYC 2 cut(s) 248, 980
FspBI CTAG 7 cut(s) 27, 345, 629, 1319, 1424, 1751, 1881
GsuI CTGGAG 1 cut(s) 1976
HaeIII GGCC 7 cut(s) 5, 169, 674, 699, 1372, 1413, 1906
HapII CCGG 2 cut(s) 35, 211
HgaI GACGC 2 cut(s) 171, 1705
Hin1I GRCGYC 1 cut(s) 57
HinfI GANTC 7 cut(s) 770, 800, 824, 1159, 1191, 1811, 1984
HpaII CCGG 2 cut(s) 35, 211
HphI GGTGA 8 cut(s) 368, 500, 506, 929, 1083, 1383, 1911, 1984
Hpy166II GTNNAC 4 cut(s) 782, 1183, 1284, 1324
Hpy188III TCNNGA 6 cut(s) 380, 482, 637, 767, 1808, 1955
Hpy8I GTNNAC 4 cut(s) 782, 1183, 1284, 1324
HpyCH4III ACNGT 5 cut(s) 473, 536, 705, 1036, 1136
HpyCH4IV ACGT 2 cut(s) 57, 115
HpyF10VI GCNNNNNNNGC 6 cut(s) 185, 878, 881, 1228, 1534, 1679
HpyF3I CTNAG 6 cut(s) 624, 828, 877, 1721, 1760, 1847
HpySE526I ACGT 2 cut(s) 57, 115
Hsp92I GRCGYC 1 cut(s) 57
KpnI GGTACC 1 cut(s) 599
Ksp22I TGATCA 1 cut(s) 763
Kzo9I GATC 9 cut(s) 86, 269, 333, 376, 420, 763, 1556, 1765, 1804
LmnI GCTCC 4 cut(s) 67, 251, 975, 983
Lsp1109I GCAGC 9 cut(s) 166, 457, 805, 827, 859, 893, 1058, 1136, 1214
LweI GCATC 5 cut(s) 274, 893, 1031, 1458, 1753
MaeI CTAG 7 cut(s) 27, 345, 629, 1319, 1424, 1751, 1881
MaeII ACGT 2 cut(s) 57, 115
MaeIII GTNAC 8 cut(s) 479, 709, 787, 902, 1089, 1332, 1378, 1972
MalI GATC 9 cut(s) 88, 271, 335, 378, 422, 765, 1558, 1767, 1806
MbiI CCGCTC 2 cut(s) 354, 1962
MboI GATC 9 cut(s) 86, 269, 333, 376, 420, 763, 1556, 1765, 1804
MfeI CAATTG 1 cut(s) 397
MhlI GDGCHC 5 cut(s) 248, 311, 980, 1248, 1675
MluCI AATT 8 cut(s) 236, 397, 617, 759, 867, 988, 1223, 1735
MlyI GAGTC 3 cut(s) 764, 818, 1805
MmeI TCCRAC 1 cut(s) 978
Mph1103I ATGCAT 1 cut(s) 1473
MroXI GAANNNNTTC 1 cut(s) 932
MseI TTAA 4 cut(s) 1016, 1541, 1733, 1999
MslI CAYNNNNRTG 2 cut(s) 486, 1933
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 2 cut(s) 35, 211
MspR9I CCNGG 3 cut(s) 647, 1342, 1433
MunI CAATTG 1 cut(s) 397
Mva1269I GAATGC 1 cut(s) 838
MvaI CCWGG 3 cut(s) 647, 1342, 1433
MvnI CGCG 1 cut(s) 901
MwoI GCNNNNNNNGC 6 cut(s) 185, 878, 881, 1228, 1534, 1679
NcoI CCATGG 2 cut(s) 216, 599
NdeI CATATG 1 cut(s) 652
NdeII GATC 9 cut(s) 86, 269, 333, 376, 420, 763, 1556, 1765, 1804
NheI GCTAGC 1 cut(s) 628
NlaIV GGNNCC 2 cut(s) 597, 1940
NmuCI GTSAC 5 cut(s) 479, 902, 1089, 1378, 1972
NsiI ATGCAT 1 cut(s) 1473
OliI CACNNNNGTG 1 cut(s) 486
PaeR7I CTCGAG 1 cut(s) 1813
PagI TCATGA 2 cut(s) 379, 1807
PceI AGGCCT 1 cut(s) 169
PctI GAATGC 1 cut(s) 838
PdmI GAANNNNTTC 1 cut(s) 932
PfeI GAWTC 4 cut(s) 800, 1159, 1191, 1984
Ple19I CGATCG 1 cut(s) 89
PleI GAGTC 3 cut(s) 764, 818, 1805
PpsI GAGTC 3 cut(s) 764, 818, 1805
PsiI TTATAA 1 cut(s) 993
Psp124BI GAGCTC 2 cut(s) 248, 980
Psp6I CCWGG 3 cut(s) 645, 1340, 1431
PspEI GGTNACC 1 cut(s) 1089
PspGI CCWGG 3 cut(s) 645, 1340, 1431
PspN4I GGNNCC 2 cut(s) 597, 1940
PspPI GGNCC 1 cut(s) 213
PspXI VCTCGAGB 1 cut(s) 1813
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 827
PvuI CGATCG 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 818
RgaI GCGATCGC 1 cut(s) 89
RsaI GTAC 9 cut(s) 538, 597, 783, 1138, 1176, 1217, 1592, 1706, 1792
RsaNI GTAC 9 cut(s) 537, 596, 782, 1137, 1175, 1216, 1591, 1705, 1791
RseI CAYNNNNRTG 2 cut(s) 486, 1933
SacI GAGCTC 2 cut(s) 248, 980
SaqAI TTAA 4 cut(s) 1016, 1541, 1733, 1999
Sau3AI GATC 9 cut(s) 86, 269, 333, 376, 420, 763, 1556, 1765, 1804
Sau96I GGNCC 1 cut(s) 213
SchI GAGTC 3 cut(s) 764, 818, 1805
ScrFI CCNGG 3 cut(s) 647, 1342, 1433
SduI GDGCHC 5 cut(s) 248, 311, 980, 1248, 1675
SfaAI GCGATCGC 1 cut(s) 89
SfaNI GCATC 5 cut(s) 274, 893, 1031, 1458, 1753
SfcI CTRYAG 4 cut(s) 51, 701, 819, 1770
Sfr274I CTCGAG 1 cut(s) 1813
SgfI GCGATCGC 1 cut(s) 89
SinI GGWCC 1 cut(s) 213
SlaI CTCGAG 1 cut(s) 1813
SmiMI CAYNNNNRTG 2 cut(s) 486, 1933
SmlI CTYRAG 3 cut(s) 637, 1532, 1813
SmoI CTYRAG 3 cut(s) 637, 1532, 1813
Sse9I AATT 8 cut(s) 236, 397, 617, 759, 867, 988, 1223, 1735
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 6 cut(s) 354, 1643, 1742, 1748, 1907, 1962
SspI AATATT 3 cut(s) 124, 1587, 1611
SspMI CTAG 7 cut(s) 27, 345, 629, 1319, 1424, 1751, 1881
SstI GAGCTC 2 cut(s) 248, 980
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 3 cut(s) 645, 1340, 1431
StyI CCWWGG 3 cut(s) 216, 599, 1662
TaaI ACNGT 5 cut(s) 473, 536, 705, 1036, 1136
TaiI ACGT 2 cut(s) 60, 118
TaqI TCGA 6 cut(s) 242, 336, 1242, 1440, 1555, 1814
TaqII GACCGA 1 cut(s) 1077
TasI AATT 8 cut(s) 236, 397, 617, 759, 867, 988, 1223, 1735
TatI WGTACW 4 cut(s) 536, 1136, 1174, 1590
TauI GCSGC 2 cut(s) 1909, 1965
TfiI GAWTC 4 cut(s) 800, 1159, 1191, 1984
Tru1I TTAA 4 cut(s) 1016, 1541, 1733, 1999
Tru9I TTAA 4 cut(s) 1016, 1541, 1733, 1999
TscAI CASTG 2 cut(s) 478, 1291
TseFI GTSAC 5 cut(s) 479, 902, 1089, 1378, 1972
TseI GCWGC 9 cut(s) 179, 445, 815, 818, 872, 881, 1046, 1149, 1202
Tsp45I GTSAC 5 cut(s) 479, 902, 1089, 1378, 1972
TspRI CASTG 2 cut(s) 478, 1291
VpaK11BI GGWCC 1 cut(s) 213
XapI RAATTY 1 cut(s) 1735
XhoI CTCGAG 1 cut(s) 1813
XmnI GAANNNNTTC 1 cut(s) 932
XspI CTAG 7 cut(s) 27, 345, 629, 1319, 1424, 1751, 1881
ZraI GACGTC 1 cut(s) 58
Zsp2I ATGCAT 1 cut(s) 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.