Rh3BG111500

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002962
Physical Location & Seq
Reverse (-)
9106994 .. 9109392
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG111500.1

Sequence Viewer

Length: 1797 bp
ATGGCATTGACTTCATTAGCTATTCCTAGCTCTCTGGCTTCATCAACAAAATATATCCTCAAAGAGTACAATGACCTTCACCAGTCACAATCTAGCAGAGACCTATGCAATCTTGCTAGTATCCAGGGAAGGTTTTCTTCAGCCGATAACCAGATTTGTTATTGCATCAACCGTATCCATGCAACATCAACAGAACCAGAACCAAAGAAGAGTCCTTTCGTGCGAATTTCCTCGTTTCAAAGTCGGGGTTCTATCATTCCAAATTTGCTACGATCATATTCATTTGATCCAAACTCGAGTAAGCATATGAACATGGTGGAGAATGACAGAAATGAGGTGGAGAGCTCTCGGGAGAGCAGTGAAGCGGAGAAACTAAAGAGAACCAGTTGGGTGGAGAGTCTTGCAGAACTCGGCAGAAACAACTGGAAGAATATTTGGCAGCAAAAAGTGGTTGGGGGTGAAGACAACGACTGTGACGAGGAAAGGAAACGCAGATCATATGATCGTGACCTTTTCTCGAGATTTTTGGTTGGAGTACCATGGTCAGATACCAAATTATTCTCTCAGCTAGCCTTCTTGTGCAACTTGGCTTATGTGATACCAGAGATCAGGGCCACGGATTTAAAGAGATACTATGGCCTACAATTTGTAACATCTTCATTAGATAAGCAAGCAAAGGCAGATAGAATTGTGAGTATCAAATCAAAACGTGATCAGGACTCTACTGCAGTGAATTCTATGGCCTCAGCTGCAGCTTATCTCCGCTCAGTAAGCAAATCACAGCAGGAGGTTGCTGGAGAGAATTCACGCAGAAATGGAGGGAGCTCACCCCAAAATCATAAGTCAGAGATAGATGCTTCCCTTACAGCATCAACAATGACGTTGAGGCTCACAGCAGGTAATGAGGAAAACCAAGGAGCAGCAAAGGATATTCAGTCACTGCACTCCTTACCTTGTGAGTGGTTTGTTTGTGATGAGCCCACTACATATACTCGTTTCTTTGTGATTCAGGGTATAGACTCCCTTGCATCCTGGCAGGCATATTTCTTATTTGAACCCATTGAATTTGAGGGAACTAGTGTACTTGTTCACCGAGGAATTTATGAACTGGCAAAGGAAATTTACCAGCAGTTCAAACCCAAAATAATTGACCACTTGGCAAGATATGGGGAGCAAGCGAAGCTTCAATTTACTGGCCATTGTCTTGGAGGAAGCCTTTCTGTTATAGTCCACTTGATGCTACTGACTAGGAAGCTCGTCAAACCCTCAACTCTTCTGCCTGTTGTCACATTTGGCTCCCCATATGTGTTCTGTGGAGGCCAGAAAATATTTGAGCAACTAGGATTTGATGAGTATGATCACATCCGTTGTGTTATGCTGCACAGAGATATTGTCCCTAGATCTTTCTCCTCTAACTACCCTAATCATGTTGCAGAAGTTCTTAAGCGTTTACACAGTTCATTTGAGTCACACCCTTGTCTAACAAAGAATAAGATATTGTACTCTCCTATGGGTAGACTATTTATCGTCCAACCTGATGAAAAGTCATCTCCTCCACACCCTCTACTCCCTTCGGGGAGCTCTCTCTATGCTTTGGACATTACCCAATGTGGATTCTCCTCGAATGTCTTTAGGGCCTTTTTGAATTCCCCACATCCACTAGAAACACTAAGTGATCCTACAGCGTATGGTTCGGAAGGTACAATTCTAAGGGACCATGACTCGAGCAACTATCTAAAGGCTGTGAATGGGGTCCTAATTAAGGCTATACAAAAGGAAGGTTGTCAGGAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

598

Amino Acids

67.22

Weight (kDa)

7.57

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 334 - 470 7.4e-22 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 887
AccBSI CCGCTC 1 cut(s) 765
AccI GTMKAC 1 cut(s) 1516
AciI CCGC 2 cut(s) 365, 763
AclWI GGATC 2 cut(s) 281, 1670
AcoI YGGCCR 1 cut(s) 1195
AcsI RAATTY 8 cut(s) 225, 262, 733, 802, 1064, 1098, 1119, 1645
AcuI CTGAAG 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 1673
AfaI GTAC 5 cut(s) 68, 537, 1083, 1502, 1702
AfiI CCNNNNNNNGG 1 cut(s) 1762
AflII CTTAAG 1 cut(s) 1442
AgsI TTSAA 6 cut(s) 239, 1055, 1064, 1135, 1187, 1645
AhlI ACTAGT 1 cut(s) 1076
AjnI CCWGG 2 cut(s) 123, 1031
Alw21I GWGCWC 3 cut(s) 347, 827, 1583
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 2 cut(s) 281, 1670
AlwNI CAGNNNCTG 1 cut(s) 940
Ama87I CYCGRG 4 cut(s) 295, 348, 517, 1723
AoxI GGCC 6 cut(s) 612, 637, 741, 1195, 1318, 1635
ApeKI GCWGC 5 cut(s) 439, 749, 752, 920, 1378
ApoI RAATTY 8 cut(s) 225, 262, 733, 802, 1064, 1098, 1119, 1645
Asp700I GAANNNNTTC 2 cut(s) 133, 1216
AspS9I GGNCC 4 cut(s) 612, 1635, 1714, 1753
AsuHPI GGTGA 4 cut(s) 71, 470, 819, 1082
AsuNHI GCTAGC 1 cut(s) 568
AvaI CYCGRG 4 cut(s) 295, 348, 517, 1723
AvaII GGWCC 2 cut(s) 1714, 1753
BalI TGGCCA 1 cut(s) 1197
BanII GRGCYC 4 cut(s) 347, 827, 981, 1583
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 3 cut(s) 347, 827, 1583
BbvCI CCTCAGC 1 cut(s) 745
BbvI GCAGC 5 cut(s) 451, 736, 764, 932, 1365
BciT130I CCWGG 2 cut(s) 125, 1033
BciVI GTATCC 2 cut(s) 131, 185
BclI TGATCA 2 cut(s) 712, 1357
BcoDI GTCTC 1 cut(s) 93
BcuI ACTAGT 1 cut(s) 1076
BfmI CTRYAG 3 cut(s) 726, 750, 1680
BfrI CTTAAG 1 cut(s) 1442
BfuAI ACCTGC 1 cut(s) 887
BfuI GTATCC 2 cut(s) 131, 185
BglII AGATCT 1 cut(s) 1400
BisI GCNGC 5 cut(s) 440, 750, 753, 921, 1379
BlsI GCNGC 5 cut(s) 441, 751, 754, 922, 1380
Bme1390I CCNGG 2 cut(s) 125, 1033
Bme18I GGWCC 2 cut(s) 1714, 1753
BmeT110I CYCGRG 4 cut(s) 295, 348, 517, 1723
BmgT120I GGNCC 4 cut(s) 612, 1635, 1714, 1753
BmiI GGNNCC 3 cut(s) 1297, 1715, 1754
BmrFI CCNGG 2 cut(s) 125, 1033
BmsI GCATC 5 cut(s) 174, 844, 878, 1037, 1227
BmtI GCTAGC 1 cut(s) 572
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 816
Bpu10I CCTNAGC 1 cut(s) 745
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 5 cut(s) 124, 539, 615, 913, 1093
Bsc4I CCNNNNNNNGG 1 cut(s) 1762
Bse1I ACTGG 5 cut(s) 82, 384, 428, 1113, 1198
BseBI CCWGG 2 cut(s) 125, 1033
BseDI CCNNGG 5 cut(s) 124, 539, 615, 913, 1093
BseGI GGATG 3 cut(s) 1028, 1362, 1654
BseLI CCNNNNNNNGG 1 cut(s) 1762
BseMII CTCAG 3 cut(s) 578, 759, 780
BseNI ACTGG 5 cut(s) 82, 384, 428, 1113, 1198
BseRI GAGGAG 3 cut(s) 1399, 1542, 1609
BseXI GCAGC 5 cut(s) 451, 736, 764, 932, 1365
BsgI GTGCAG 2 cut(s) 926, 1364
BshFI GGCC 6 cut(s) 614, 639, 743, 1197, 1320, 1637
BsiHKAI GWGCWC 3 cut(s) 347, 827, 1583
BsiHKCI CYCGRG 4 cut(s) 295, 348, 517, 1723
BslFI GGGAC 2 cut(s) 1379, 1727
BslI CCNNNNNNNGG 1 cut(s) 1762
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 1379, 1727
BsnI GGCC 6 cut(s) 614, 639, 743, 1197, 1320, 1637
Bso31I GGTCTC 1 cut(s) 93
BsoBI CYCGRG 4 cut(s) 295, 348, 517, 1723
Bsp1286I GDGCHC 4 cut(s) 347, 827, 981, 1583
Bsp143I GATC 9 cut(s) 272, 286, 494, 502, 606, 712, 1357, 1400, 1675
Bsp19I CCATGG 1 cut(s) 539
BspACI CCGC 2 cut(s) 365, 763
BspANI GGCC 6 cut(s) 614, 639, 743, 1197, 1320, 1637
BspCNI CTCAG 3 cut(s) 577, 758, 779
BspLI GGNNCC 3 cut(s) 1297, 1715, 1754
BspMAI CTGCAG 2 cut(s) 730, 754
BspMI ACCTGC 1 cut(s) 887
BspOI GCTAGC 1 cut(s) 572
BspPI GGATC 2 cut(s) 281, 1670
BspTI CTTAAG 1 cut(s) 1442
BspTNI GGTCTC 1 cut(s) 93
BsrBI CCGCTC 1 cut(s) 765
BsrI ACTGG 5 cut(s) 82, 384, 428, 1113, 1198
BssECI CCNNGG 5 cut(s) 124, 539, 615, 913, 1093
BssMI GATC 9 cut(s) 272, 286, 494, 502, 606, 712, 1357, 1400, 1675
BssT1I CCWWGG 2 cut(s) 539, 913
Bst2UI CCWGG 2 cut(s) 125, 1033
Bst4CI ACNGT 3 cut(s) 173, 473, 1457
Bst6I CTCTTC 2 cut(s) 203, 1278
BstAFI CTTAAG 1 cut(s) 1442
BstC8I GCNNGC 4 cut(s) 570, 672, 1038, 1176
BstDEI CTNAG 5 cut(s) 564, 745, 766, 1670, 1709
BstDSI CCRYGG 2 cut(s) 539, 615
BstENI CCTNNNNNAGG 1 cut(s) 1760
BstF5I GGATG 3 cut(s) 1028, 1362, 1654
BstKTI GATC 9 cut(s) 275, 289, 497, 505, 609, 715, 1360, 1403, 1678
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 9 cut(s) 272, 286, 494, 502, 606, 712, 1357, 1400, 1675
BstMWI GCNNNNNNNGC 3 cut(s) 749, 771, 1180
BstNI CCWGG 2 cut(s) 125, 1033
BstSCI CCNGG 2 cut(s) 123, 1031
BstSFI CTRYAG 3 cut(s) 726, 750, 1680
BstV1I GCAGC 5 cut(s) 451, 736, 764, 932, 1365
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 1400
BstXI CCANNNNNNTGG 2 cut(s) 391, 1205
BstYI RGATCY 1 cut(s) 1400
BsuI GTATCC 2 cut(s) 131, 185
BsuRI GGCC 6 cut(s) 614, 639, 743, 1197, 1320, 1637
BtgI CCRYGG 2 cut(s) 539, 615
BtsCI GGATG 3 cut(s) 1028, 1362, 1654
BtsI GCAGTG 3 cut(s) 364, 735, 938
BtsIMutI CAGTG 3 cut(s) 364, 735, 938
BveI ACCTGC 1 cut(s) 887
Cac8I GCNNGC 4 cut(s) 570, 672, 1038, 1176
CaiI CAGNNNCTG 1 cut(s) 940
Cfr13I GGNCC 4 cut(s) 612, 1635, 1714, 1753
Csp6I GTAC 5 cut(s) 67, 536, 1082, 1501, 1701
CviAII CATG 5 cut(s) 179, 313, 540, 1427, 1718
CviQI GTAC 5 cut(s) 67, 536, 1082, 1501, 1701
DdeI CTNAG 5 cut(s) 564, 745, 766, 1670, 1709
DpnI GATC 9 cut(s) 274, 288, 496, 504, 608, 714, 1359, 1402, 1677
DpnII GATC 9 cut(s) 272, 286, 494, 502, 606, 712, 1357, 1400, 1675
DraI TTTAAA 1 cut(s) 624
DraIII CACNNNGTG 1 cut(s) 1673
EaeI YGGCCR 1 cut(s) 1195
Eam1104I CTCTTC 2 cut(s) 203, 1278
EarI CTCTTC 2 cut(s) 203, 1278
Ecl136II GAGCTC 3 cut(s) 345, 825, 1581
Eco130I CCWWGG 2 cut(s) 539, 913
Eco24I GRGCYC 4 cut(s) 347, 827, 981, 1583
Eco31I GGTCTC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 1714, 1753
Eco53kI GAGCTC 3 cut(s) 345, 825, 1581
Eco57I CTGAAG 1 cut(s) 123
Eco88I CYCGRG 4 cut(s) 295, 348, 517, 1723
EcoICRI GAGCTC 3 cut(s) 345, 825, 1581
EcoNI CCTNNNNNAGG 1 cut(s) 1760
EcoO109I RGGNCCY 2 cut(s) 1635, 1753
EcoRI GAATTC 3 cut(s) 733, 802, 1645
EcoRII CCWGG 2 cut(s) 123, 1031
EcoT14I CCWWGG 2 cut(s) 539, 913
EcoT38I GRGCYC 4 cut(s) 347, 827, 981, 1583
ErhI CCWWGG 2 cut(s) 539, 913
FaeI CATG 5 cut(s) 182, 316, 543, 1430, 1721
FalI AAGNNNNNCTT 4 cut(s) 121, 153, 1167, 1199
FaqI GGGAC 2 cut(s) 1379, 1727
FatI CATG 5 cut(s) 178, 312, 539, 1426, 1717
FauNDI CATATG 3 cut(s) 306, 499, 1303
FbaI TGATCA 2 cut(s) 712, 1357
FblI GTMKAC 1 cut(s) 1516
Fnu4HI GCNGC 5 cut(s) 440, 750, 753, 921, 1379
FokI GGATG 3 cut(s) 1015, 1349, 1641
FriOI GRGCYC 4 cut(s) 347, 827, 981, 1583
Fsp4HI GCNGC 5 cut(s) 440, 750, 753, 921, 1379
GluI GCNGC 5 cut(s) 440, 750, 753, 921, 1379
GsuI CTGGAG 1 cut(s) 816
HaeIII GGCC 6 cut(s) 614, 639, 743, 1197, 1320, 1637
Hin1II CATG 5 cut(s) 182, 316, 543, 1430, 1721
HindIII AAGCTT 1 cut(s) 1181
HinfI GANTC 8 cut(s) 211, 397, 719, 1006, 1019, 1466, 1614, 1721
HphI GGTGA 4 cut(s) 71, 470, 819, 1082
Hpy166II GTNNAC 5 cut(s) 1082, 1090, 1231, 1451, 1517
Hpy188I TCNGA 3 cut(s) 547, 847, 1696
Hpy188III TCNNGA 6 cut(s) 350, 506, 517, 519, 716, 1787
Hpy8I GTNNAC 5 cut(s) 1082, 1090, 1231, 1451, 1517
HpyAV CCTTC 6 cut(s) 86, 123, 583, 1581, 1691, 1772
HpyCH4III ACNGT 3 cut(s) 173, 473, 1457
HpyCH4IV ACGT 2 cut(s) 709, 881
HpyF10VI GCNNNNNNNGC 3 cut(s) 749, 771, 1180
HpyF3I CTNAG 5 cut(s) 564, 745, 766, 1670, 1709
HpySE526I ACGT 2 cut(s) 709, 881
Hsp92II CATG 5 cut(s) 182, 316, 543, 1430, 1721
Ksp22I TGATCA 2 cut(s) 712, 1357
Kzo9I GATC 9 cut(s) 272, 286, 494, 502, 606, 712, 1357, 1400, 1675
LmnI GCTCC 5 cut(s) 822, 917, 1171, 1301, 1578
Lsp1109I GCAGC 5 cut(s) 451, 736, 764, 932, 1365
LweI GCATC 5 cut(s) 174, 844, 878, 1037, 1227
MaeII ACGT 2 cut(s) 709, 881
MaeIII GTNAC 7 cut(s) 84, 473, 506, 649, 936, 1285, 1467
MalI GATC 9 cut(s) 274, 288, 496, 504, 608, 714, 1359, 1402, 1677
MbiI CCGCTC 1 cut(s) 765
MboI GATC 9 cut(s) 272, 286, 494, 502, 606, 712, 1357, 1400, 1675
MboII GAAGA 6 cut(s) 129, 220, 439, 473, 648, 1265
MflI RGATCY 1 cut(s) 1400
MhlI GDGCHC 4 cut(s) 347, 827, 981, 1583
MlsI TGGCCA 1 cut(s) 1197
MluNI TGGCCA 1 cut(s) 1197
MlyI GAGTC 6 cut(s) 220, 406, 713, 1013, 1475, 1715
MmeI TCCRAC 2 cut(s) 511, 1555
Mox20I TGGCCA 1 cut(s) 1197
MroXI GAANNNNTTC 2 cut(s) 133, 1216
MscI TGGCCA 1 cut(s) 1197
MseI TTAA 3 cut(s) 623, 1443, 1761
Msp20I TGGCCA 1 cut(s) 1197
MspA1I CMGCKG 1 cut(s) 749
MspCI CTTAAG 1 cut(s) 1442
MspR9I CCNGG 2 cut(s) 125, 1033
MvaI CCWGG 2 cut(s) 125, 1033
MwoI GCNNNNNNNGC 3 cut(s) 749, 771, 1180
NcoI CCATGG 1 cut(s) 539
NdeI CATATG 3 cut(s) 306, 499, 1303
NdeII GATC 9 cut(s) 272, 286, 494, 502, 606, 712, 1357, 1400, 1675
NheI GCTAGC 1 cut(s) 568
NlaIII CATG 5 cut(s) 182, 316, 543, 1430, 1721
NlaIV GGNNCC 3 cut(s) 1297, 1715, 1754
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 6 cut(s) 84, 473, 506, 936, 1285, 1467
PaeR7I CTCGAG 3 cut(s) 295, 517, 1723
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PdmI GAANNNNTTC 2 cut(s) 133, 1216
PfeI GAWTC 2 cut(s) 1006, 1614
PkrI GCNGC 5 cut(s) 441, 751, 754, 922, 1380
PleI GAGTC 6 cut(s) 219, 405, 713, 1013, 1474, 1715
PpsI GAGTC 6 cut(s) 219, 405, 713, 1013, 1474, 1715
PpuMI RGGWCCY 1 cut(s) 1753
Psp124BI GAGCTC 3 cut(s) 347, 827, 1583
Psp5II RGGWCCY 1 cut(s) 1753
Psp6I CCWGG 2 cut(s) 123, 1031
PspGI CCWGG 2 cut(s) 123, 1031
PspN4I GGNNCC 3 cut(s) 1297, 1715, 1754
PspPI GGNCC 4 cut(s) 612, 1635, 1714, 1753
PspPPI RGGWCCY 1 cut(s) 1753
PspXI VCTCGAGB 2 cut(s) 295, 1723
PstI CTGCAG 2 cut(s) 730, 754
PstNI CAGNNNCTG 1 cut(s) 940
PsuI RGATCY 1 cut(s) 1400
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 5 cut(s) 68, 537, 1083, 1502, 1702
RsaNI GTAC 5 cut(s) 67, 536, 1082, 1501, 1701
SacI GAGCTC 3 cut(s) 347, 827, 1583
SaqAI TTAA 3 cut(s) 623, 1443, 1761
SatI GCNGC 5 cut(s) 440, 750, 753, 921, 1379
Sau3AI GATC 9 cut(s) 272, 286, 494, 502, 606, 712, 1357, 1400, 1675
Sau96I GGNCC 4 cut(s) 612, 1635, 1714, 1753
SchI GAGTC 6 cut(s) 220, 406, 713, 1013, 1475, 1715
ScrFI CCNGG 2 cut(s) 125, 1033
SduI GDGCHC 4 cut(s) 347, 827, 981, 1583
SfaNI GCATC 5 cut(s) 174, 844, 878, 1037, 1227
SfcI CTRYAG 3 cut(s) 726, 750, 1680
Sfr274I CTCGAG 3 cut(s) 295, 517, 1723
SinI GGWCC 2 cut(s) 1714, 1753
SlaI CTCGAG 3 cut(s) 295, 517, 1723
SmlI CTYRAG 4 cut(s) 295, 517, 1442, 1723
SmoI CTYRAG 4 cut(s) 295, 517, 1442, 1723
SpeI ACTAGT 1 cut(s) 1076
SsiI CCGC 2 cut(s) 365, 763
SspI AATATT 2 cut(s) 433, 1329
SstI GAGCTC 3 cut(s) 347, 827, 1583
StyD4I CCNGG 2 cut(s) 123, 1031
StyI CCWWGG 2 cut(s) 539, 913
TaaI ACNGT 3 cut(s) 173, 473, 1457
TaiI ACGT 2 cut(s) 712, 884
TaqI TCGA 4 cut(s) 296, 518, 1622, 1724
TatI WGTACW 3 cut(s) 66, 1081, 1500
TfiI GAWTC 2 cut(s) 1006, 1614
Tru1I TTAA 3 cut(s) 623, 1443, 1761
Tru9I TTAA 3 cut(s) 623, 1443, 1761
TscAI CASTG 3 cut(s) 364, 735, 945
TseFI GTSAC 6 cut(s) 84, 473, 506, 936, 1285, 1467
TseI GCWGC 5 cut(s) 439, 749, 752, 920, 1378
Tsp45I GTSAC 6 cut(s) 84, 473, 506, 936, 1285, 1467
TspDTI ATGAA 7 cut(s) 30, 270, 323, 648, 1119, 1449, 1554
TspGWI ACGGA 2 cut(s) 632, 1355
TspRI CASTG 3 cut(s) 364, 735, 945
Vha464I CTTAAG 1 cut(s) 1442
VpaK11BI GGWCC 2 cut(s) 1714, 1753
XagI CCTNNNNNAGG 1 cut(s) 1760
XapI RAATTY 8 cut(s) 225, 262, 733, 802, 1064, 1098, 1119, 1645
XhoI CTCGAG 3 cut(s) 295, 517, 1723
XmiI GTMKAC 1 cut(s) 1516
XmnI GAANNNNTTC 2 cut(s) 133, 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.