Rroxscaffold_4G00297190

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002962
Physical Location & Seq
Reverse (-)
17129613 .. 17132389
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00297190.1

Sequence Viewer

Length: 2136 bp
ATGGCCTGCACTTCATTATCCTCTCCGGCTTCAACGAGGACTATAGACGTCTTCAAGGAGCAGAGTGGTCTTCGGCGATCGCAATCCAGCAAAGACCTTTACAGACGTGCAAATATTCGGAGGTCTTATTCCGATAACCATCTTTGCTACTGCATTAGGCCTATTCACGCTGCGTCAGCCCAACCAAAACTAAAGAACAGCCGGTCCATGGGGTTTTTCCCCTTTCAATTTTCGAGCTCCATTCTCCCAAACTCGGTTAGATCATTTTTGTTTGATGCAGACACGAGTAAGCATATGAGCACGGCAGAGAGGGAAATGGAAATGATCGATAACTCTAGGGAGAGCGGTGAAGAAGAAGAAGATCATGAGATAAAGAGAGCCAATTGGGTGGAGAGGCTTGTGGAGATCAGAACAAAATGGAATAATAGGCAGCAAAAAGAAGTTGGGGATGAAGACAGTGTTTGTCACGAAGGTGATGGTGACTGTGAATGTGGAGAGGGTGGTTGTTTAGTGGATTACAGTACAGATGAAGATGATGGAGAGATAACTTCAGAAAGTTTCCAAAGGTTTTTGGTTAAGGTACCATGGTCTGATACCAAACAATTTTCTCAGCTATCCTTCTTGAGCAACCTGGCATATGTAATACCATTGATAAAGGCCGATGATTTGAAGAGATATTACGGCCTACAGTTTGTAACATCTTCATTAGTAAAGAAAGCAGAGGAAGAGGAACTCAAAGAAGGAATTGATCAGGACTCTACTCGTGTACCTGTAACTGACGAGGAATCCAGTTCAGAGGCAGCTGCAGACTCTGAGCAGAAGCATTCAATCCATCCATCTGTTGCTTACGAAATTGCTGCCTCAGCAGCATCTTATGTTCGCTCGCGTGACATGGATGGTGGTGATGTAGAGAATTCATTCAGAAGTGGAGACCAACAACAACAACGAGAGGAAAGAGGGAGCTCCCCCCGAAATTATAAGTCGGAGGTAGCTGTTTCCTTAACAGCATCAACGATGACAGTCGTGGTTGCAGCAGGGGAAAAACAAAAGCAAGAAACAGCACAAGACCTTCGGTCACCCCAATCTGCACCTTGTGAATGGTTTGTTTGTGATGACAACAGTACATATACTCGCTGCTTTGTGATTCAGGGTTCGGACTCCCTTGCGTCCTGGCAGGCAAACCTCTTCTTTGAACCTACAAAATTTGAGGTAAGCATAAGATACGTACAGAACTACTTACTGGTTGTTGTCTTGGAAGAAAATGAGAAACCGCTGAACGAACCTTATAATCTTGTACAGGGAACAAATGTGCTTGTTCACAGAGGAATCTATGAAGCAGCAAAGGGGATGTACGAGCAATTCATGCCACAGATAATCGAGCACTTGGATAGATATGGGGAGCGTGCAAAGTTTCAGTTCACTGGCCATTCTCTTGGAGGAAGCCTCTCTCTTCTAGTCCACTTGATGTTACTGACCAGGAACTTTGTCAAGCCCTCCACTCTTCGGCCAGTTGTGACATTTGGCTCGCCATTTGTGTTTTGTGGAGGCCAGAAAATACTAGACGACCTGGGTGTGGATGAGAACCAAATCCATTGCGTGATGATGCATAGAGATATTGTCCCAAGAGCCTTCTCCTGCAACTATCCTAACCATGTGGCTACGCTTCTCAAGCGTTTAAATGGTTCATTTCGATCACACCCTTGTCTGATAAAAAACAAAATTTTGTACTCTCCTATGGGTAAAATATTCATTCTGCAACCTGATGAAAACTCATCTCCGCCGCACCCTCTTCTCCCCGTTGGGAGTGCCCTCTATGCTTTGGACAAGACTCAACATGGGTACTCTTCAAGCGTCCTTAGAACCTTCTTAAATTCCCCGCATCCACTAGAAACCCTAAGCGATCCTACAGCTTATGGTTCGGAAGGTACAATCCTACGGGATCATGACTCGAGCAACTATCTGAAGGCTATGAATGGTGTCCTTAGGCTACACACAAGAAAGGTTGTTCGTATAGCTAGGAAACAGAGGAATGTCTTGTGGCCGCTACTTACTTCACCATCCCCACACACATGGAACCATGAAAATAATCTGGAGAGCGGCAGACTGGTGACGAAGGAGATTCTCACAGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

711

Amino Acids

80.21

Weight (kDa)

5.86

Isoelectric Point (pI)

55.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 380 - 547 4.6e-23 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 978, 1287
AatII GACGTC 1 cut(s) 51
Acc65I GGTACC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 580
AccBSI CCGCTC 2 cut(s) 345, 2097
AccII CGCG 1 cut(s) 886
AciI CCGC 7 cut(s) 345, 1271, 1778, 1781, 1877, 2042, 2097
AclWI GGATC 2 cut(s) 1895, 1947
AcoI YGGCCR 3 cut(s) 1423, 1505, 2039
AcsI RAATTY 4 cut(s) 913, 1202, 1719, 1870
AcuI CTGAAG 2 cut(s) 534, 1982
AcyI GRCGYC 1 cut(s) 48
AdeI CACNNNGTG 1 cut(s) 1094
AfiI CCNNNNNNNGG 4 cut(s) 208, 253, 1503, 1573
AgsI TTSAA 7 cut(s) 33, 55, 227, 670, 828, 1193, 1848
AhdI GACNNNNNGTC 1 cut(s) 1072
AjiI CACGTC 1 cut(s) 107
AjnI CCWGG 4 cut(s) 630, 1169, 1475, 1566
AleI CACNNNNGTG 1 cut(s) 471
AloI GAACNNNNNNTCC 2 cut(s) 188, 220
AluBI AGCT 7 cut(s) 237, 613, 803, 963, 992, 1910, 2015
AluI AGCT 7 cut(s) 237, 613, 803, 963, 992, 1910, 2015
Alw21I GWGCWC 4 cut(s) 239, 302, 965, 1383
Alw26I GTCTC 1 cut(s) 924
AlwI GGATC 2 cut(s) 1895, 1947
AlwNI CAGNNNCTG 1 cut(s) 812
Ama87I CYCGRG 1 cut(s) 1948
AoxI GGCC 8 cut(s) 3, 158, 657, 682, 1423, 1505, 1546, 2039
ApeKI GCWGC 9 cut(s) 170, 430, 800, 803, 857, 866, 1031, 1134, 1337
ApoI RAATTY 4 cut(s) 913, 1202, 1719, 1870
AsiSI GCGATCGC 1 cut(s) 80
Asp700I GAANNNNTTC 1 cut(s) 917
Asp718I GGTACC 1 cut(s) 580
AspS9I GGNCC 1 cut(s) 204
AsuHPI GGTGA 7 cut(s) 359, 485, 491, 914, 1068, 2046, 2119
AvaI CYCGRG 1 cut(s) 1948
AvaII GGWCC 1 cut(s) 204
AxyI CCTNAGG 1 cut(s) 1982
BaeGI GKGCMC 1 cut(s) 1810
BaeI ACNNNNGTAYC 4 cut(s) 572, 605, 750, 783
BalI TGGCCA 1 cut(s) 1425
BanI GGYRCC 1 cut(s) 580
BanII GRGCYC 2 cut(s) 239, 965
BarI GAAGNNNNNNTAC 2 cut(s) 662, 694
BauI CACGAG 2 cut(s) 283, 762
BbsI GAAGAC 3 cut(s) 43, 62, 459
Bbv12I GWGCWC 4 cut(s) 239, 302, 965, 1383
BbvCI CCTCAGC 1 cut(s) 862
BbvI GCAGC 9 cut(s) 157, 442, 790, 812, 844, 878, 1043, 1121, 1349
BccI CCATC 7 cut(s) 147, 470, 530, 840, 844, 890, 2065
BceAI ACGGC 2 cut(s) 318, 697
BcgI CGANNNNNNTGC 2 cut(s) 839, 873
BciT130I CCWGG 4 cut(s) 632, 1171, 1477, 1568
BclI TGATCA 1 cut(s) 748
BcoDI GTCTC 1 cut(s) 924
BfaI CTAG 5 cut(s) 336, 1454, 1559, 1886, 2016
BfmI CTRYAG 4 cut(s) 42, 686, 804, 1905
Bme1390I CCNGG 4 cut(s) 632, 1171, 1477, 1568
Bme18I GGWCC 1 cut(s) 204
BmeRI GACNNNNNGTC 1 cut(s) 1072
BmeT110I CYCGRG 1 cut(s) 1948
BmgBI CACGTC 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 204
BmiI GGNNCC 2 cut(s) 582, 2075
BmrFI CCNGG 4 cut(s) 632, 1171, 1477, 1568
BmsI GCATC 5 cut(s) 265, 878, 1016, 1593, 1888
BpiI GAAGAC 3 cut(s) 43, 62, 459
BplI GAGNNNNNCTC 2 cut(s) 1428, 1460
BpmI CTGGAG 1 cut(s) 2111
Bpu10I CCTNAGC 2 cut(s) 862, 1895
BpuEI CTTGAG 2 cut(s) 643, 1652
Bsa29I ATCGAT 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 1225
BsaBI GATNNNNATC 2 cut(s) 82, 138
BsaHI GRCGYC 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 924
BsaJI CCNNGG 3 cut(s) 207, 584, 1567
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 253, 1503, 1573
Bse118I RCCGGY 1 cut(s) 201
Bse1I ACTGG 5 cut(s) 789, 1245, 1426, 1508, 2109
Bse21I CCTNAGG 1 cut(s) 1982
Bse3DI GCAATG 1 cut(s) 1591
Bse8I GATNNNNATC 2 cut(s) 82, 138
BseBI CCWGG 4 cut(s) 632, 1171, 1477, 1568
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 3 cut(s) 207, 584, 1567
BseGI GGATG 7 cut(s) 454, 832, 901, 1353, 1582, 1879, 2057
BseJI GATNNNNATC 2 cut(s) 82, 138
BseLI CCNNNNNNNGG 4 cut(s) 208, 253, 1503, 1573
BseMI GCAATG 1 cut(s) 1591
BseMII CTCAG 3 cut(s) 623, 804, 876
BseNI ACTGG 5 cut(s) 789, 1245, 1426, 1508, 2109
BseSI GKGCMC 1 cut(s) 1810
BseXI GCAGC 9 cut(s) 157, 442, 790, 812, 844, 878, 1043, 1121, 1349
BsgI GTGCAG 1 cut(s) 1071
Bsh1236I CGCG 1 cut(s) 886
Bsh1285I CGRYCG 1 cut(s) 80
BshFI GGCC 8 cut(s) 5, 160, 659, 684, 1425, 1507, 1548, 2041
BshNI GGYRCC 1 cut(s) 580
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 80
BsiHKAI GWGCWC 4 cut(s) 239, 302, 965, 1383
BsiHKCI CYCGRG 1 cut(s) 1948
BsiSI CCGG 2 cut(s) 26, 202
BslFI GGGAC 1 cut(s) 1604
BslI CCNNNNNNNGG 4 cut(s) 208, 253, 1503, 1573
BsmAI GTCTC 1 cut(s) 924
BsmFI GGGAC 1 cut(s) 1604
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 8 cut(s) 5, 160, 659, 684, 1425, 1507, 1548, 2041
Bso31I GGTCTC 1 cut(s) 924
BsoBI CYCGRG 1 cut(s) 1948
Bsp1286I GDGCHC 5 cut(s) 239, 302, 965, 1383, 1810
Bsp1407I TGTACA 1 cut(s) 1294
Bsp143I GATC 9 cut(s) 77, 260, 324, 361, 405, 748, 1691, 1900, 1939
Bsp19I CCATGG 2 cut(s) 207, 584
BspACI CCGC 7 cut(s) 345, 1271, 1778, 1781, 1877, 2042, 2097
BspANI GGCC 8 cut(s) 5, 160, 659, 684, 1425, 1507, 1548, 2041
BspCNI CTCAG 3 cut(s) 622, 805, 875
BspDI ATCGAT 1 cut(s) 327
BspFNI CGCG 1 cut(s) 886
BspHI TCATGA 2 cut(s) 364, 1942
BspLI GGNNCC 2 cut(s) 582, 2075
BspMAI CTGCAG 1 cut(s) 808
BspPI GGATC 2 cut(s) 1895, 1947
BspT107I GGYRCC 1 cut(s) 580
BspTNI GGTCTC 1 cut(s) 924
BsrBI CCGCTC 2 cut(s) 345, 2097
BsrDI GCAATG 1 cut(s) 1591
BsrFI RCCGGY 1 cut(s) 201
BsrGI TGTACA 1 cut(s) 1294
BsrI ACTGG 5 cut(s) 789, 1245, 1426, 1508, 2109
BssAI RCCGGY 1 cut(s) 201
BssECI CCNNGG 3 cut(s) 207, 584, 1567
BssMI GATC 9 cut(s) 77, 260, 324, 361, 405, 748, 1691, 1900, 1939
BssNI GRCGYC 1 cut(s) 48
BssSI CACGAG 2 cut(s) 283, 762
BssT1I CCWWGG 2 cut(s) 207, 584
Bst2BI CACGAG 2 cut(s) 283, 762
Bst2UI CCWGG 4 cut(s) 632, 1171, 1477, 1568
Bst4CI ACNGT 6 cut(s) 458, 485, 521, 690, 1021, 1121
Bst6I CTCTTC 7 cut(s) 665, 720, 1190, 1455, 1506, 1794, 1849
BstACI GRCGYC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 1363
BstAUI TGTACA 1 cut(s) 1294
BstBAI YACGTR 1 cut(s) 1225
BstC8I GCNNGC 5 cut(s) 7, 884, 1176, 1404, 1526
BstDEI CTNAG 6 cut(s) 609, 813, 862, 1856, 1895, 1982
BstDSI CCRYGG 2 cut(s) 207, 584
BstEII GGTNACC 1 cut(s) 1074
BstF5I GGATG 7 cut(s) 454, 832, 901, 1353, 1582, 1879, 2057
BstFNI CGCG 1 cut(s) 886
BstKTI GATC 9 cut(s) 80, 263, 327, 364, 408, 751, 1694, 1903, 1942
BstMAI GTCTC 1 cut(s) 924
BstMBI GATC 9 cut(s) 77, 260, 324, 361, 405, 748, 1691, 1900, 1939
BstMCI CGRYCG 1 cut(s) 80
BstMWI GCNNNNNNNGC 6 cut(s) 176, 863, 866, 1363, 1669, 1814
BstNI CCWGG 4 cut(s) 632, 1171, 1477, 1568
BstPI GGTNACC 1 cut(s) 1074
BstSCI CCNGG 4 cut(s) 630, 1169, 1475, 1566
BstSFI CTRYAG 4 cut(s) 42, 686, 804, 1905
BstSLI GKGCMC 1 cut(s) 1810
BstSNI TACGTA 1 cut(s) 1225
BstUI CGCG 1 cut(s) 886
BstV1I GCAGC 9 cut(s) 157, 442, 790, 812, 844, 878, 1043, 1121, 1349
BstV2I GAAGAC 3 cut(s) 43, 62, 459
BstXI CCANNNNNNTGG 3 cut(s) 388, 1433, 2070
Bsu15I ATCGAT 1 cut(s) 327
Bsu36I CCTNAGG 1 cut(s) 1982
BsuRI GGCC 8 cut(s) 5, 160, 659, 684, 1425, 1507, 1548, 2041
BsuTUI ATCGAT 1 cut(s) 327
BtgI CCRYGG 2 cut(s) 207, 584
BtrI CACGTC 1 cut(s) 107
BtsCI GGATG 7 cut(s) 454, 832, 901, 1353, 1582, 1879, 2057
BtsIMutI CAGTG 2 cut(s) 463, 1419
Cac8I GCNNGC 5 cut(s) 7, 884, 1176, 1404, 1526
CaiI CAGNNNCTG 1 cut(s) 812
CciI TCATGA 2 cut(s) 364, 1942
Cfr10I RCCGGY 1 cut(s) 201
Cfr13I GGNCC 1 cut(s) 204
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 3 cut(s) 162, 1155, 1840
DdeI CTNAG 6 cut(s) 609, 813, 862, 1856, 1895, 1982
DpnI GATC 9 cut(s) 79, 262, 326, 363, 407, 750, 1693, 1902, 1941
DpnII GATC 9 cut(s) 77, 260, 324, 361, 405, 748, 1691, 1900, 1939
DraI TTTAAA 1 cut(s) 1677
DraIII CACNNNGTG 1 cut(s) 1094
DriI GACNNNNNGTC 1 cut(s) 1072
EaeI YGGCCR 3 cut(s) 1423, 1505, 2039
Eam1104I CTCTTC 7 cut(s) 665, 720, 1190, 1455, 1506, 1794, 1849
Eam1105I GACNNNNNGTC 1 cut(s) 1072
EarI CTCTTC 7 cut(s) 665, 720, 1190, 1455, 1506, 1794, 1849
EciI GGCGGA 1 cut(s) 1767
Ecl136II GAGCTC 2 cut(s) 237, 963
Eco105I TACGTA 1 cut(s) 1225
Eco130I CCWWGG 2 cut(s) 207, 584
Eco147I AGGCCT 1 cut(s) 160
Eco24I GRGCYC 2 cut(s) 239, 965
Eco31I GGTCTC 1 cut(s) 924
Eco47I GGWCC 1 cut(s) 204
Eco53kI GAGCTC 2 cut(s) 237, 963
Eco57I CTGAAG 2 cut(s) 534, 1982
Eco81I CCTNAGG 1 cut(s) 1982
Eco88I CYCGRG 1 cut(s) 1948
Eco91I GGTNACC 1 cut(s) 1074
EcoICRI GAGCTC 2 cut(s) 237, 963
EcoO65I GGTNACC 1 cut(s) 1074
EcoRI GAATTC 1 cut(s) 913
EcoRII CCWGG 4 cut(s) 630, 1169, 1475, 1566
EcoT14I CCWWGG 2 cut(s) 207, 584
EcoT22I ATGCAT 1 cut(s) 1608
EcoT38I GRGCYC 2 cut(s) 239, 965
ErhI CCWWGG 2 cut(s) 207, 584
FaqI GGGAC 1 cut(s) 1604
FauI CCCGC 1 cut(s) 1884
FauNDI CATATG 2 cut(s) 294, 637
FbaI TGATCA 1 cut(s) 748
FokI GGATG 7 cut(s) 461, 819, 908, 1360, 1589, 1866, 2044
FriOI GRGCYC 2 cut(s) 239, 965
FspBI CTAG 5 cut(s) 336, 1454, 1559, 1886, 2016
GsuI CTGGAG 1 cut(s) 2111
HaeIII GGCC 8 cut(s) 5, 160, 659, 684, 1425, 1507, 1548, 2041
HapII CCGG 2 cut(s) 26, 202
HgaI GACGC 3 cut(s) 162, 1155, 1840
Hin1I GRCGYC 1 cut(s) 48
HinfI GANTC 9 cut(s) 755, 785, 809, 1144, 1157, 1326, 1828, 1946, 2119
HpaII CCGG 2 cut(s) 26, 202
HphI GGTGA 7 cut(s) 359, 485, 491, 914, 1068, 2046, 2119
Hpy166II GTNNAC 4 cut(s) 767, 1318, 1419, 1459
Hpy188III TCNNGA 6 cut(s) 365, 467, 622, 752, 1943, 2090
Hpy8I GTNNAC 4 cut(s) 767, 1318, 1419, 1459
HpyAV CCTTC 9 cut(s) 464, 628, 734, 1079, 1639, 1873, 1916, 1957, 2107
HpyCH4III ACNGT 6 cut(s) 458, 485, 521, 690, 1021, 1121
HpyCH4IV ACGT 3 cut(s) 48, 106, 1224
HpyF10VI GCNNNNNNNGC 6 cut(s) 176, 863, 866, 1363, 1669, 1814
HpyF3I CTNAG 6 cut(s) 609, 813, 862, 1856, 1895, 1982
HpySE526I ACGT 3 cut(s) 48, 106, 1224
Hsp92I GRCGYC 1 cut(s) 48
KpnI GGTACC 1 cut(s) 584
Ksp22I TGATCA 1 cut(s) 748
Kzo9I GATC 9 cut(s) 77, 260, 324, 361, 405, 748, 1691, 1900, 1939
LmnI GCTCC 5 cut(s) 58, 242, 960, 968, 1399
Lsp1109I GCAGC 9 cut(s) 157, 442, 790, 812, 844, 878, 1043, 1121, 1349
LweI GCATC 5 cut(s) 265, 878, 1016, 1593, 1888
MaeI CTAG 5 cut(s) 336, 1454, 1559, 1886, 2016
MaeII ACGT 3 cut(s) 48, 106, 1224
MaeIII GTNAC 9 cut(s) 464, 479, 694, 772, 887, 1074, 1467, 1513, 2107
MalI GATC 9 cut(s) 79, 262, 326, 363, 407, 750, 1693, 1902, 1941
MbiI CCGCTC 2 cut(s) 345, 2097
MboI GATC 9 cut(s) 77, 260, 324, 361, 405, 748, 1691, 1900, 1939
MfeI CAATTG 1 cut(s) 382
MhlI GDGCHC 5 cut(s) 239, 302, 965, 1383, 1810
MlsI TGGCCA 1 cut(s) 1425
MluNI TGGCCA 1 cut(s) 1425
MlyI GAGTC 5 cut(s) 749, 803, 1151, 1822, 1940
MmeI TCCRAC 1 cut(s) 963
Mox20I TGGCCA 1 cut(s) 1425
Mph1103I ATGCAT 1 cut(s) 1608
MroXI GAANNNNTTC 1 cut(s) 917
MscI TGGCCA 1 cut(s) 1425
MseI TTAA 5 cut(s) 576, 1001, 1676, 1868, 2134
MslI CAYNNNNRTG 2 cut(s) 471, 2068
Msp20I TGGCCA 1 cut(s) 1425
MspA1I CMGCKG 2 cut(s) 803, 1273
MspI CCGG 2 cut(s) 26, 202
MspR9I CCNGG 4 cut(s) 632, 1171, 1477, 1568
MunI CAATTG 1 cut(s) 382
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 4 cut(s) 632, 1171, 1477, 1568
MvnI CGCG 1 cut(s) 886
MwoI GCNNNNNNNGC 6 cut(s) 176, 863, 866, 1363, 1669, 1814
NcoI CCATGG 2 cut(s) 207, 584
NdeI CATATG 2 cut(s) 294, 637
NdeII GATC 9 cut(s) 77, 260, 324, 361, 405, 748, 1691, 1900, 1939
NlaIV GGNNCC 2 cut(s) 582, 2075
NmuCI GTSAC 6 cut(s) 464, 479, 887, 1074, 1513, 2107
NsiI ATGCAT 1 cut(s) 1608
OliI CACNNNNGTG 1 cut(s) 471
PaeR7I CTCGAG 1 cut(s) 1948
PagI TCATGA 2 cut(s) 364, 1942
PceI AGGCCT 1 cut(s) 160
PctI GAATGC 1 cut(s) 823
PdmI GAANNNNTTC 1 cut(s) 917
PfeI GAWTC 4 cut(s) 785, 1144, 1326, 2119
Ple19I CGATCG 1 cut(s) 80
PleI GAGTC 5 cut(s) 749, 803, 1151, 1822, 1940
PpsI GAGTC 5 cut(s) 749, 803, 1151, 1822, 1940
Ppu21I YACGTR 1 cut(s) 1225
PsiI TTATAA 2 cut(s) 978, 1287
Psp124BI GAGCTC 2 cut(s) 239, 965
Psp6I CCWGG 4 cut(s) 630, 1169, 1475, 1566
PspEI GGTNACC 1 cut(s) 1074
PspGI CCWGG 4 cut(s) 630, 1169, 1475, 1566
PspN4I GGNNCC 2 cut(s) 582, 2075
PspPI GGNCC 1 cut(s) 204
PspXI VCTCGAGB 1 cut(s) 1948
PstI CTGCAG 1 cut(s) 808
PstNI CAGNNNCTG 1 cut(s) 812
PvuI CGATCG 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 803
RgaI GCGATCGC 1 cut(s) 80
RseI CAYNNNNRTG 2 cut(s) 471, 2068
SacI GAGCTC 2 cut(s) 239, 965
SaqAI TTAA 5 cut(s) 576, 1001, 1676, 1868, 2134
Sau3AI GATC 9 cut(s) 77, 260, 324, 361, 405, 748, 1691, 1900, 1939
Sau96I GGNCC 1 cut(s) 204
SchI GAGTC 5 cut(s) 749, 803, 1151, 1822, 1940
ScrFI CCNGG 4 cut(s) 632, 1171, 1477, 1568
SduI GDGCHC 5 cut(s) 239, 302, 965, 1383, 1810
SfaAI GCGATCGC 1 cut(s) 80
SfaNI GCATC 5 cut(s) 265, 878, 1016, 1593, 1888
SfcI CTRYAG 4 cut(s) 42, 686, 804, 1905
Sfr274I CTCGAG 1 cut(s) 1948
SgfI GCGATCGC 1 cut(s) 80
SinI GGWCC 1 cut(s) 204
SlaI CTCGAG 1 cut(s) 1948
SmiMI CAYNNNNRTG 2 cut(s) 471, 2068
SmlI CTYRAG 3 cut(s) 622, 1667, 1948
SmoI CTYRAG 3 cut(s) 622, 1667, 1948
SnaBI TACGTA 1 cut(s) 1225
SseBI AGGCCT 1 cut(s) 160
SsiI CCGC 7 cut(s) 345, 1271, 1778, 1781, 1877, 2042, 2097
SspI AATATT 2 cut(s) 115, 1746
SspMI CTAG 5 cut(s) 336, 1454, 1559, 1886, 2016
SstI GAGCTC 2 cut(s) 239, 965
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 4 cut(s) 630, 1169, 1475, 1566
StyI CCWWGG 2 cut(s) 207, 584
TaaI ACNGT 6 cut(s) 458, 485, 521, 690, 1021, 1121
TaiI ACGT 3 cut(s) 51, 109, 1227
TaqI TCGA 5 cut(s) 233, 327, 1377, 1690, 1949
TaqII GACCGA 1 cut(s) 1062
TatI WGTACW 4 cut(s) 521, 1121, 1294, 1725
TauI GCSGC 3 cut(s) 1783, 2044, 2100
TfiI GAWTC 4 cut(s) 785, 1144, 1326, 2119
Tru1I TTAA 5 cut(s) 576, 1001, 1676, 1868, 2134
Tru9I TTAA 5 cut(s) 576, 1001, 1676, 1868, 2134
TscAI CASTG 2 cut(s) 463, 1426
TseFI GTSAC 6 cut(s) 464, 479, 887, 1074, 1513, 2107
TseI GCWGC 9 cut(s) 170, 430, 800, 803, 857, 866, 1031, 1134, 1337
Tsp45I GTSAC 6 cut(s) 464, 479, 887, 1074, 1513, 2107
TspRI CASTG 2 cut(s) 463, 1426
VpaK11BI GGWCC 1 cut(s) 204
XapI RAATTY 4 cut(s) 913, 1202, 1719, 1870
XhoI CTCGAG 1 cut(s) 1948
XmnI GAANNNNTTC 1 cut(s) 917
XspI CTAG 5 cut(s) 336, 1454, 1559, 1886, 2016
ZraI GACGTC 1 cut(s) 49
Zsp2I ATGCAT 1 cut(s) 1608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.