Rorug01G0268900

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002962
Physical Location & Seq
Reverse (-)
38131293 .. 38132691
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0268900.1

Sequence Viewer

Length: 1029 bp
ATGGGTACACTGGTCAGTTCTTCAAATCTCTCATATCTCCGAGAACAAGAAGAAAAGATGGGATCTTTGAGTACGGAAATCCTCCAGGACAAGAAATTATGCGAGGACTGGTCTGCGCTTCATATGTTGGTAGAGGTTTCCTGCCATGTTAGAGAAGAAGAAAAGAAGGGTATGTGTGCGCTATCCTTGGTGAGTCAACAGGTATGCCCTAAGAAACAAAGGTCGCCACCCCGTGGTGAAAGTTCAAAAATGACGACCCCTATCCCTGATATTGCTTCTGGTTCTTCCGATCAAGAAAATAGAAGAATTGATAGCAAGGGTTTGAAAAGAAAAAGGAAGCCATGTGTTGACTGTCATAATGATAAAGACTTGGATGCCGCGGCGAAGAAGAAGAAGAAGAGAACATATATGATGAAGGTGGACATGGGTGTTTATCAACCACCTCCTGATTTGCCTGAAAAGTACAAGCAAATGGTGAATGGGGCAAAGGCGGCATTGGTGATACAAAAGCAGTTGTTTACGAGTGATGTGAACCCTGACCTGAACCGGCTGACATTGCCTCCAAAACAAATTTTGTGCAAGGAGTTGCTGAGACCTGATGAGATAGAGATCCTTAAGCCGAAGGATGCTTTGATTAAAGTTCCATTTATAGACCCAGAGCTCAGAAAGGAAGAGATTTCACTGAGTTTATGGAAGCTGTCGAGCTCAAATGAAAGGTCCCTTGTGTTCAAATCTAAATGGAATCATATTGTTAAGAAGAACGATCTGGGTGAAGGTGATCTGATCCAAATCTGGTCATTTCGGGATAGCAACGATCAACTTCAGTTGGCACTTGTTGTGGTGAAGAGAGCCGAGAAGCGCAAAAATGGCGAGGAAGTGAGAAGCACCACAAGAAGTAGTAGTGTGAGCAATGTCAGTGAGGACAGTGCAAGTGCAAGCCACAGTGGAAGAAAATGTCTCAAAATCAGATTTAAATGTAATGCTAGCAGGAGTGAGAGTACTAGTACATGTGAATTATTAAGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

342

Amino Acids

38.66

Weight (kDa)

9.25

Isoelectric Point (pI)

61.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 155 - 253 7.4e-13 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 233
AccII CGCG 1 cut(s) 380
AciI CCGC 3 cut(s) 378, 380, 491
AclWI GGATC 3 cut(s) 70, 604, 778
AcsI RAATTY 1 cut(s) 570
AcuI CTGAAG 1 cut(s) 806
AdeI CACNNNGTG 1 cut(s) 233
AfaI GTAC 5 cut(s) 7, 73, 464, 1000, 1006
AfiI CCNNNNNNNGG 1 cut(s) 233
AflII CTTAAG 1 cut(s) 614
AflIII ACRYGT 1 cut(s) 1007
AgsI TTSAA 4 cut(s) 24, 246, 325, 730
AhlI ACTAGT 1 cut(s) 1001
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 3 cut(s) 661, 697, 705
AluI AGCT 3 cut(s) 661, 697, 705
Alw21I GWGCWC 2 cut(s) 663, 707
Alw26I GTCTC 2 cut(s) 586, 962
AlwI GGATC 3 cut(s) 70, 604, 778
ApoI RAATTY 1 cut(s) 570
ArsI GACNNNNNNTTYG 2 cut(s) 701, 733
AspLEI GCGC 3 cut(s) 118, 181, 861
AspS9I GGNCC 1 cut(s) 717
AsuHPI GGTGA 7 cut(s) 202, 248, 487, 511, 782, 788, 853
AsuNHI GCTAGC 1 cut(s) 983
AvaII GGWCC 1 cut(s) 717
BaeI ACNNNNGTAYC 1 cut(s) 30
BanII GRGCYC 2 cut(s) 663, 707
Bbv12I GWGCWC 2 cut(s) 663, 707
BccI CCATC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 86
BcoDI GTCTC 2 cut(s) 586, 962
BcuI ACTAGT 1 cut(s) 1001
BfaI CTAG 2 cut(s) 984, 1002
BfrI CTTAAG 1 cut(s) 614
BisI GCNGC 3 cut(s) 378, 381, 492
BlsI GCNGC 3 cut(s) 379, 382, 493
BmcAI AGTACT 1 cut(s) 1000
Bme1390I CCNGG 1 cut(s) 86
Bme18I GGWCC 1 cut(s) 717
BmgT120I GGNCC 1 cut(s) 717
BmiI GGNNCC 1 cut(s) 719
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 2 cut(s) 364, 616
BmtI GCTAGC 1 cut(s) 987
BpmI CTGGAG 1 cut(s) 68
BsaBI GATNNNNATC 2 cut(s) 608, 788
BsaI GGTCTC 1 cut(s) 586
BsaJI CCNNGG 3 cut(s) 186, 232, 378
BsaXI ACNNNNNCTCC 4 cut(s) 544, 574, 982, 1012
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse118I RCCGGY 1 cut(s) 546
Bse1I ACTGG 2 cut(s) 15, 113
Bse3DI GCAATG 2 cut(s) 554, 916
Bse8I GATNNNNATC 2 cut(s) 608, 788
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 3 cut(s) 186, 232, 378
BseGI GGATG 2 cut(s) 379, 631
BseJI GATNNNNATC 2 cut(s) 608, 788
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMI GCAATG 2 cut(s) 554, 916
BseMII CTCAG 3 cut(s) 581, 674, 676
BseNI ACTGG 2 cut(s) 15, 113
Bsh1236I CGCG 1 cut(s) 380
BsiHKAI GWGCWC 2 cut(s) 663, 707
BsiSI CCGG 1 cut(s) 547
BslFI GGGAC 1 cut(s) 703
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 2 cut(s) 586, 962
BsmFI GGGAC 1 cut(s) 703
Bso31I GGTCTC 1 cut(s) 586
Bsp1286I GDGCHC 2 cut(s) 663, 707
Bsp143I GATC 7 cut(s) 62, 289, 609, 763, 778, 783, 814
BspACI CCGC 3 cut(s) 378, 380, 491
BspCNI CTCAG 3 cut(s) 582, 675, 675
BspFNI CGCG 1 cut(s) 380
BspLI GGNNCC 1 cut(s) 719
BspOI GCTAGC 1 cut(s) 987
BspPI GGATC 3 cut(s) 70, 604, 778
BspTI CTTAAG 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 586
BsrDI GCAATG 2 cut(s) 554, 916
BsrFI RCCGGY 1 cut(s) 546
BsrI ACTGG 2 cut(s) 15, 113
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 3 cut(s) 186, 232, 378
BssMI GATC 7 cut(s) 62, 289, 609, 763, 778, 783, 814
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 3 cut(s) 353, 926, 944
Bst6I CTCTTC 3 cut(s) 392, 666, 839
BstAFI CTTAAG 1 cut(s) 614
BstC8I GCNNGC 2 cut(s) 937, 985
BstDEI CTNAG 4 cut(s) 210, 590, 662, 683
BstDSI CCRYGG 2 cut(s) 232, 378
BstF5I GGATG 2 cut(s) 379, 631
BstFNI CGCG 1 cut(s) 380
BstHHI GCGC 3 cut(s) 118, 181, 861
BstKTI GATC 7 cut(s) 65, 292, 612, 766, 781, 786, 817
BstMAI GTCTC 2 cut(s) 586, 962
BstMBI GATC 7 cut(s) 62, 289, 609, 763, 778, 783, 814
BstMWI GCNNNNNNNGC 3 cut(s) 491, 556, 867
BstNI CCWGG 1 cut(s) 86
BstNSI RCATGY 1 cut(s) 1011
BstSCI CCNGG 1 cut(s) 84
BstUI CGCG 1 cut(s) 380
BstX2I RGATCY 2 cut(s) 62, 609
BstYI RGATCY 2 cut(s) 62, 609
BtgI CCRYGG 2 cut(s) 232, 378
BtsCI GGATG 2 cut(s) 379, 631
BtsIMutI CAGTG 5 cut(s) 8, 680, 922, 931, 949
Cac8I GCNNGC 2 cut(s) 937, 985
CfoI GCGC 3 cut(s) 118, 181, 861
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 1 cut(s) 717
Cfr42I CCGCGG 1 cut(s) 381
Csp6I GTAC 5 cut(s) 6, 72, 463, 999, 1005
CviAII CATG 4 cut(s) 146, 342, 424, 1008
CviJI RGCY 8 cut(s) 340, 550, 619, 661, 697, 705, 851, 939
CviKI_1 RGCY 8 cut(s) 340, 550, 619, 661, 697, 705, 851, 939
CviQI GTAC 5 cut(s) 6, 72, 463, 999, 1005
DdeI CTNAG 4 cut(s) 210, 590, 662, 683
DpnI GATC 7 cut(s) 64, 291, 611, 765, 780, 785, 816
DpnII GATC 7 cut(s) 62, 289, 609, 763, 778, 783, 814
DraI TTTAAA 1 cut(s) 973
DraIII CACNNNGTG 1 cut(s) 233
Eam1104I CTCTTC 3 cut(s) 392, 666, 839
EarI CTCTTC 3 cut(s) 392, 666, 839
Ecl136II GAGCTC 2 cut(s) 661, 705
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 2 cut(s) 663, 707
Eco31I GGTCTC 1 cut(s) 586
Eco47I GGWCC 1 cut(s) 717
Eco53kI GAGCTC 2 cut(s) 661, 705
Eco57I CTGAAG 1 cut(s) 806
EcoICRI GAGCTC 2 cut(s) 661, 705
EcoO109I RGGNCCY 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 2 cut(s) 663, 707
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 4 cut(s) 149, 345, 427, 1011
FaqI GGGAC 1 cut(s) 703
FatI CATG 4 cut(s) 145, 341, 423, 1007
FauNDI CATATG 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 378, 381, 492
FokI GGATG 2 cut(s) 386, 638
FriOI GRGCYC 2 cut(s) 663, 707
Fsp4HI GCNGC 3 cut(s) 378, 381, 492
FspBI CTAG 2 cut(s) 984, 1002
GlaI GCGC 3 cut(s) 117, 180, 860
GluI GCNGC 3 cut(s) 378, 381, 492
GsuI CTGGAG 1 cut(s) 68
HapII CCGG 1 cut(s) 547
HhaI GCGC 3 cut(s) 118, 181, 861
Hin1II CATG 4 cut(s) 149, 345, 427, 1011
Hin6I GCGC 3 cut(s) 116, 179, 859
HinP1I GCGC 3 cut(s) 116, 179, 859
HincII GTYRAC 2 cut(s) 197, 349
HindII GTYRAC 2 cut(s) 197, 349
HinfI GANTC 2 cut(s) 193, 742
HpaII CCGG 1 cut(s) 547
HphI GGTGA 7 cut(s) 202, 248, 487, 511, 782, 788, 853
Hpy166II GTNNAC 6 cut(s) 8, 197, 349, 421, 519, 532
Hpy188I TCNGA 5 cut(s) 41, 289, 665, 783, 968
Hpy188III TCNNGA 3 cut(s) 293, 446, 803
Hpy8I GTNNAC 6 cut(s) 8, 197, 349, 421, 519, 532
HpyAV CCTTC 4 cut(s) 160, 409, 616, 767
HpyCH4III ACNGT 3 cut(s) 353, 926, 944
HpyCH4V TGCA 3 cut(s) 579, 929, 935
HpyF10VI GCNNNNNNNGC 3 cut(s) 491, 556, 867
HpyF3I CTNAG 4 cut(s) 210, 590, 662, 683
Hsp92II CATG 4 cut(s) 149, 345, 427, 1011
HspAI GCGC 3 cut(s) 116, 179, 859
KspI CCGCGG 1 cut(s) 381
Kzo9I GATC 7 cut(s) 62, 289, 609, 763, 778, 783, 814
LweI GCATC 2 cut(s) 364, 616
MaeI CTAG 2 cut(s) 984, 1002
MalI GATC 7 cut(s) 64, 291, 611, 765, 780, 785, 816
MboI GATC 7 cut(s) 62, 289, 609, 763, 778, 783, 814
MflI RGATCY 2 cut(s) 62, 609
MhlI GDGCHC 2 cut(s) 663, 707
MluCI AATT 4 cut(s) 95, 306, 570, 1013
MlyI GAGTC 1 cut(s) 202
MnlI CCTC 7 cut(s) 92, 97, 127, 453, 570, 865, 913
MseI TTAA 5 cut(s) 615, 636, 753, 972, 1019
MspA1I CMGCKG 1 cut(s) 380
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 1 cut(s) 547
MspR9I CCNGG 1 cut(s) 86
MvaI CCWGG 1 cut(s) 86
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 3 cut(s) 491, 556, 867
NdeI CATATG 1 cut(s) 123
NdeII GATC 7 cut(s) 62, 289, 609, 763, 778, 783, 814
NheI GCTAGC 1 cut(s) 983
NlaIII CATG 4 cut(s) 149, 345, 427, 1011
NlaIV GGNNCC 1 cut(s) 719
NmeAIII GCCGAG 1 cut(s) 877
NspI RCATGY 1 cut(s) 1011
PciI ACATGT 1 cut(s) 1007
PfeI GAWTC 1 cut(s) 742
PflMI CCANNNNNTGG 1 cut(s) 233
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 3 cut(s) 379, 382, 493
PleI GAGTC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 201
PpuMI RGGWCCY 1 cut(s) 717
PscI ACATGT 1 cut(s) 1007
Psp124BI GAGCTC 2 cut(s) 663, 707
Psp5II RGGWCCY 1 cut(s) 717
Psp6I CCWGG 1 cut(s) 84
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 1 cut(s) 719
PspPI GGNCC 1 cut(s) 717
PspPPI RGGWCCY 1 cut(s) 717
PsuI RGATCY 2 cut(s) 62, 609
RsaI GTAC 5 cut(s) 7, 73, 464, 1000, 1006
RsaNI GTAC 5 cut(s) 6, 72, 463, 999, 1005
SacI GAGCTC 2 cut(s) 663, 707
SacII CCGCGG 1 cut(s) 381
SaqAI TTAA 5 cut(s) 615, 636, 753, 972, 1019
SatI GCNGC 3 cut(s) 378, 381, 492
Sau3AI GATC 7 cut(s) 62, 289, 609, 763, 778, 783, 814
Sau96I GGNCC 1 cut(s) 717
ScaI AGTACT 1 cut(s) 1000
SchI GAGTC 1 cut(s) 202
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 2 cut(s) 663, 707
SfaNI GCATC 2 cut(s) 364, 616
Sfr303I CCGCGG 1 cut(s) 381
SgrBI CCGCGG 1 cut(s) 381
SinI GGWCC 1 cut(s) 717
SmiI ATTTAAAT 1 cut(s) 973
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
SpeI ACTAGT 1 cut(s) 1001
Sse9I AATT 4 cut(s) 95, 306, 570, 1013
SsiI CCGC 3 cut(s) 378, 380, 491
SspMI CTAG 2 cut(s) 984, 1002
SstI GAGCTC 2 cut(s) 663, 707
StyD4I CCNGG 1 cut(s) 84
StyI CCWWGG 1 cut(s) 186
SwaI ATTTAAAT 1 cut(s) 973
TaaI ACNGT 3 cut(s) 353, 926, 944
TaqI TCGA 1 cut(s) 701
TasI AATT 4 cut(s) 95, 306, 570, 1013
TatI WGTACW 3 cut(s) 462, 998, 1004
TauI GCSGC 3 cut(s) 380, 383, 494
TfiI GAWTC 1 cut(s) 742
Tru1I TTAA 5 cut(s) 615, 636, 753, 972, 1019
Tru9I TTAA 5 cut(s) 615, 636, 753, 972, 1019
TscAI CASTG 5 cut(s) 15, 687, 922, 931, 949
TspDTI ATGAA 3 cut(s) 110, 428, 726
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 5 cut(s) 15, 687, 922, 931, 949
Van91I CCANNNNNTGG 1 cut(s) 233
Vha464I CTTAAG 1 cut(s) 614
VpaK11BI GGWCC 1 cut(s) 717
XapI RAATTY 1 cut(s) 570
XceI RCATGY 1 cut(s) 1011
XspI CTAG 2 cut(s) 984, 1002
ZrmI AGTACT 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.